Rw5G015510

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001629
Physical Location & Seq
Reverse (-)
19245796 .. 19247678
1883 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G015510.1

Sequence Viewer

Length: 1185 bp
ATGGTGACAGAAATTCGAACAGATTCTTTGGACTACCGGACGGAGTTGTTGTCGCCGGACCTGACAGCAGGGAGTGTGACACTGCCAAGTGATTCGCCGGCATGGCGGCTCAATATGGATGGAGTCAAGCTTCCAACTGAAAGAAACAATATGAGTTCTTGTTTTGGCATAGGCTCTTATGTTGAGTCATTAAGGAGGCAAAAGAAAGTTGCAGAATATTACAAGAGGCAGAATAAACTTCTCAAAGGATTCAATGAATTAGACTCATTTTCTGAATTGGGTTCTTGGCCTGGAAGTTTAACAGAGCGAAAGATGGCAATATATGCATCGAATGCAGCTAACTTGGTGCTGTTCCTAGCAAAAGTGTATGCTTCAGTTGAGAGCAGATCCTTGGCAGTTATAGCTTCAACTTTGGATTCCCTCCTAGACCTCTTGTCAGGGTTCATCCTTTGGTTTACAGCTCATGCAATGAGAAAACCAAATCAGTATAGCTACCCGATTGGCAAGCATCGGATGCAGCCCGTGGGAATTGTTGTTTTTGCATCAGTAATGGCAACTCTTGGACTACAAATTTTGTTTGAATCGGGGCGGCAACTTGTCAGTAAGACTCAACCTGATAAGGACCCGGAGAAAGAAAAATGGATGATAGGGATCATGGTCTCTGCCACAGTAATCAAGTTCATTCTAATGGCATACTGCCGCAGATTCAAAAACGAGATTGTTCAAGCATATGCTCAAGATCATCTATTTGATGTCATTACTAATGGAATTGGTCTTGCATCCGCAGTTTTAGCAATCAGGTTTTATTGGTGGATCGATCCTGTTGGAGCTATCATTATTGCTTTGTATACAATTGGGAATTGGGCAAACACTGTGATGGAGAATGTGTGGTCACTAATTGGCAAGACAGCACCAGCAGAGTACTTGGCCAAGTTGACATATCTAATTTGGAACCATGACAAGCAGATCAAGCACATTGACACAGTGAGAGCATATACATTTGGTATTAACTACTTTGTGGAGGTCGACATAGTCTTACCCGGAGAGATGTCCCTTAGTGAAGCACATAATATTGGAGAGGCACTCCAAGAAAAGCTTGAGAATCTTCCTGAGGTTGAACGAGCTTTTGTTCATGTGGATGTTGATATTACTCACAAGCCAGAGCACAAGCCAAAGAGTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux ZT_dimer

Protein Analysis

394

Amino Acids

44.43

Weight (kDa)

6.77

Isoelectric Point (pI)

40.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_efflux PF01545 109 - 299 1.7e-33 Cation efflux transmembrane domain
ZT_dimer PF16916 312 - 380 6.3e-09 Cation efflux protein, cytoplasmic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 848, 1026
AciI CCGC 4 cut(s) 106, 589, 700, 783
AclWI GGATC 4 cut(s) 381, 659, 812, 821
AcoI YGGCCR 1 cut(s) 927
AcsI RAATTY 2 cut(s) 12, 570
AcuI CTGAAG 1 cut(s) 357
AfaI GTAC 2 cut(s) 923, 1180
AgsI TTSAA 6 cut(s) 253, 408, 581, 709, 725, 1118
AhdI GACNNNNNGTC 1 cut(s) 433
AjnI CCWGG 1 cut(s) 289
AluBI AGCT 8 cut(s) 130, 338, 404, 461, 492, 830, 1096, 1124
AluI AGCT 8 cut(s) 130, 338, 404, 461, 492, 830, 1096, 1124
Alw21I GWGCWC 1 cut(s) 1167
Alw26I GTCTC 1 cut(s) 664
AlwI GGATC 4 cut(s) 381, 659, 812, 821
AoxI GGCC 2 cut(s) 287, 927
ApeKI GCWGC 2 cut(s) 335, 517
ApoI RAATTY 2 cut(s) 12, 570
Asp700I GAANNNNTTC 1 cut(s) 22
AspS9I GGNCC 2 cut(s) 58, 622
AsuC2I CCSGG 2 cut(s) 626, 1041
AsuHPI GGTGA 1 cut(s) 16
AsuII TTCGAA 1 cut(s) 16
AvaII GGWCC 2 cut(s) 58, 622
AxyI CCTNAGG 1 cut(s) 1110
BalI TGGCCA 1 cut(s) 929
Bbv12I GWGCWC 1 cut(s) 1167
BbvI GCAGC 2 cut(s) 347, 529
BccI CCATC 3 cut(s) 113, 307, 871
BciT130I CCWGG 1 cut(s) 291
BcnI CCSGG 2 cut(s) 626, 1041
BcoDI GTCTC 1 cut(s) 664
BfaI CTAG 2 cut(s) 356, 425
BglI GCCNNNNNGGC 1 cut(s) 103
BisI GCNGC 5 cut(s) 107, 336, 518, 590, 700
BlsI GCNGC 5 cut(s) 108, 337, 519, 591, 701
BmcAI AGTACT 1 cut(s) 923
Bme1390I CCNGG 3 cut(s) 291, 626, 1041
Bme18I GGWCC 2 cut(s) 58, 622
BmeRI GACNNNNNGTC 1 cut(s) 433
BmgT120I GGNCC 2 cut(s) 58, 622
BmiI GGNNCC 2 cut(s) 624, 953
BmrFI CCNGG 3 cut(s) 291, 626, 1041
BmsI GCATC 5 cut(s) 335, 504, 517, 551, 788
BplI GAGNNNNNCTC 2 cut(s) 1068, 1100
Bpu14I TTCGAA 1 cut(s) 16
BpuEI CTTGAG 2 cut(s) 720, 1118
BpuMI CCSGG 2 cut(s) 626, 1041
Bsa29I ATCGAT 1 cut(s) 816
BsaBI GATNNNNATC 1 cut(s) 650
BsaI GGTCTC 1 cut(s) 664
BsaJI CCNNGG 2 cut(s) 390, 522
BsaWI WCCGGW 1 cut(s) 36
BsaXI ACNNNNNCTCC 4 cut(s) 35, 65, 872, 902
Bse118I RCCGGY 1 cut(s) 97
Bse21I CCTNAGG 1 cut(s) 1110
Bse3DI GCAATG 1 cut(s) 474
Bse8I GATNNNNATC 1 cut(s) 650
BseBI CCWGG 1 cut(s) 291
BseCI ATCGAT 1 cut(s) 816
BseDI CCNNGG 2 cut(s) 390, 522
BseGI GGATG 6 cut(s) 124, 444, 519, 648, 779, 1144
BseJI GATNNNNATC 1 cut(s) 650
BseMI GCAATG 1 cut(s) 474
BseMII CTCAG 1 cut(s) 1101
BseXI GCAGC 2 cut(s) 347, 529
BshFI GGCC 2 cut(s) 289, 929
BshVI ATCGAT 1 cut(s) 816
BsiHKAI GWGCWC 1 cut(s) 1167
BsiSI CCGG 5 cut(s) 37, 56, 98, 626, 1041
BslFI GGGAC 1 cut(s) 1036
BsmAI GTCTC 1 cut(s) 664
BsmFI GGGAC 1 cut(s) 1036
BsmI GAATGC 1 cut(s) 337
BsnI GGCC 2 cut(s) 289, 929
Bso31I GGTCTC 1 cut(s) 664
Bsp119I TTCGAA 1 cut(s) 16
Bsp1286I GDGCHC 1 cut(s) 1167
Bsp143I GATC 6 cut(s) 386, 651, 739, 813, 817, 966
BspACI CCGC 4 cut(s) 106, 589, 700, 783
BspANI GGCC 2 cut(s) 289, 929
BspCNI CTCAG 1 cut(s) 1102
BspDI ATCGAT 1 cut(s) 816
BspLI GGNNCC 2 cut(s) 624, 953
BspPI GGATC 4 cut(s) 381, 659, 812, 821
BspT104I TTCGAA 1 cut(s) 16
BspTNI GGTCTC 1 cut(s) 664
BsrDI GCAATG 1 cut(s) 474
BsrFI RCCGGY 1 cut(s) 97
BssAI RCCGGY 1 cut(s) 97
BssECI CCNNGG 2 cut(s) 390, 522
BssMI GATC 6 cut(s) 386, 651, 739, 813, 817, 966
BssNAI GTATAC 1 cut(s) 849
BssT1I CCWWGG 1 cut(s) 390
Bst1107I GTATAC 1 cut(s) 849
Bst2UI CCWGG 1 cut(s) 291
Bst4CI ACNGT 3 cut(s) 670, 874, 985
BstAPI GCANNNNNTGC 3 cut(s) 323, 332, 514
BstBI TTCGAA 1 cut(s) 16
BstC8I GCNNGC 2 cut(s) 99, 506
BstDEI CTNAG 2 cut(s) 1055, 1110
BstDSI CCRYGG 1 cut(s) 522
BstF5I GGATG 6 cut(s) 124, 444, 519, 648, 779, 1144
BstKTI GATC 6 cut(s) 389, 654, 742, 816, 820, 969
BstMAI GTCTC 1 cut(s) 664
BstMBI GATC 6 cut(s) 386, 651, 739, 813, 817, 966
BstMWI GCNNNNNNNGC 7 cut(s) 103, 323, 332, 401, 514, 791, 970
BstNI CCWGG 1 cut(s) 291
BstSCI CCNGG 3 cut(s) 289, 624, 1039
BstV1I GCAGC 2 cut(s) 347, 529
BstX2I RGATCY 1 cut(s) 386
BstYI RGATCY 1 cut(s) 386
BstZ17I GTATAC 1 cut(s) 849
Bsu15I ATCGAT 1 cut(s) 816
Bsu36I CCTNAGG 1 cut(s) 1110
BsuRI GGCC 2 cut(s) 289, 929
BsuTUI ATCGAT 1 cut(s) 816
BtgI CCRYGG 1 cut(s) 522
BtsCI GGATG 6 cut(s) 124, 444, 519, 648, 779, 1144
BtsI GCAGTG 1 cut(s) 80
BtsIMutI CAGTG 3 cut(s) 80, 870, 990
Cac8I GCNNGC 2 cut(s) 99, 506
Cfr10I RCCGGY 1 cut(s) 97
Cfr13I GGNCC 2 cut(s) 58, 622
ClaI ATCGAT 1 cut(s) 816
Csp6I GTAC 2 cut(s) 922, 1179
CviAII CATG 5 cut(s) 102, 464, 655, 956, 1133
CviQI GTAC 2 cut(s) 922, 1179
DdeI CTNAG 2 cut(s) 1055, 1110
DpnI GATC 6 cut(s) 388, 653, 741, 815, 819, 968
DpnII GATC 6 cut(s) 386, 651, 739, 813, 817, 966
DriI GACNNNNNGTC 1 cut(s) 433
EaeI YGGCCR 1 cut(s) 927
Eam1105I GACNNNNNGTC 1 cut(s) 433
Eco130I CCWWGG 1 cut(s) 390
Eco31I GGTCTC 1 cut(s) 664
Eco47I GGWCC 2 cut(s) 58, 622
Eco57I CTGAAG 1 cut(s) 357
Eco81I CCTNAGG 1 cut(s) 1110
EcoO109I RGGNCCY 1 cut(s) 622
EcoRII CCWGG 1 cut(s) 289
EcoT14I CCWWGG 1 cut(s) 390
EcoT22I ATGCAT 1 cut(s) 328
ErhI CCWWGG 1 cut(s) 390
FaeI CATG 5 cut(s) 105, 467, 658, 959, 1136
FalI AAGNNNNNCTT 2 cut(s) 1080, 1112
FaqI GGGAC 1 cut(s) 1036
FatI CATG 5 cut(s) 101, 463, 654, 955, 1132
FauNDI CATATG 1 cut(s) 730
FblI GTMKAC 2 cut(s) 848, 1026
Fnu4HI GCNGC 5 cut(s) 107, 336, 518, 590, 700
FokI GGATG 6 cut(s) 131, 431, 526, 655, 766, 1151
Fsp4HI GCNGC 5 cut(s) 107, 336, 518, 590, 700
FspBI CTAG 2 cut(s) 356, 425
GluI GCNGC 5 cut(s) 107, 336, 518, 590, 700
HaeIII GGCC 2 cut(s) 289, 929
HapII CCGG 5 cut(s) 37, 56, 98, 626, 1041
Hin1II CATG 5 cut(s) 105, 467, 658, 959, 1136
HincII GTYRAC 2 cut(s) 936, 1027
HindII GTYRAC 2 cut(s) 936, 1027
HindIII AAGCTT 2 cut(s) 128, 1094
HpaII CCGG 5 cut(s) 37, 56, 98, 626, 1041
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 4 cut(s) 456, 849, 936, 1027
Hpy188I TCNGA 2 cut(s) 274, 513
Hpy188III TCNNGA 2 cut(s) 737, 1109
Hpy8I GTNNAC 4 cut(s) 456, 849, 936, 1027
HpyCH4III ACNGT 3 cut(s) 670, 874, 985
HpyCH4V TGCA 7 cut(s) 212, 326, 335, 467, 517, 542, 779
HpyF10VI GCNNNNNNNGC 7 cut(s) 103, 323, 332, 401, 514, 791, 970
HpyF3I CTNAG 2 cut(s) 1055, 1110
Hsp92II CATG 5 cut(s) 105, 467, 658, 959, 1136
KroI GCCGGC 1 cut(s) 97
KroNI GCCGGC 1 cut(s) 99
Kzo9I GATC 6 cut(s) 386, 651, 739, 813, 817, 966
LmnI GCTCC 1 cut(s) 827
Lsp1109I GCAGC 2 cut(s) 347, 529
LweI GCATC 5 cut(s) 335, 504, 517, 551, 788
MaeI CTAG 2 cut(s) 356, 425
MaeIII GTNAC 3 cut(s) 4, 76, 891
MalI GATC 6 cut(s) 388, 653, 741, 815, 819, 968
MboI GATC 6 cut(s) 386, 651, 739, 813, 817, 966
MboII GAAGA 1 cut(s) 1097
MfeI CAATTG 1 cut(s) 852
MflI RGATCY 1 cut(s) 386
MhlI GDGCHC 1 cut(s) 1167
MlsI TGGCCA 1 cut(s) 929
MluNI TGGCCA 1 cut(s) 929
MlyI GAGTC 4 cut(s) 132, 194, 257, 601
MmeI TCCRAC 2 cut(s) 158, 805
MnlI CCTC 7 cut(s) 189, 219, 431, 440, 1015, 1072, 1105
Mox20I TGGCCA 1 cut(s) 929
Mph1103I ATGCAT 1 cut(s) 328
MroNI GCCGGC 1 cut(s) 97
MroXI GAANNNNTTC 1 cut(s) 22
MscI TGGCCA 1 cut(s) 929
MseI TTAA 3 cut(s) 191, 299, 1008
MslI CAYNNNNRTG 3 cut(s) 686, 875, 1137
Msp20I TGGCCA 1 cut(s) 929
MspI CCGG 5 cut(s) 37, 56, 98, 626, 1041
MspR9I CCNGG 3 cut(s) 291, 626, 1041
MunI CAATTG 1 cut(s) 852
Mva1269I GAATGC 1 cut(s) 337
MvaI CCWGG 1 cut(s) 291
MwoI GCNNNNNNNGC 7 cut(s) 103, 323, 332, 401, 514, 791, 970
NaeI GCCGGC 1 cut(s) 99
NciI CCSGG 2 cut(s) 626, 1041
NdeI CATATG 1 cut(s) 730
NdeII GATC 6 cut(s) 386, 651, 739, 813, 817, 966
NgoMIV GCCGGC 1 cut(s) 97
NlaIII CATG 5 cut(s) 105, 467, 658, 959, 1136
NlaIV GGNNCC 2 cut(s) 624, 953
NmuCI GTSAC 3 cut(s) 4, 76, 891
NsiI ATGCAT 1 cut(s) 328
NspV TTCGAA 1 cut(s) 16
PctI GAATGC 1 cut(s) 337
PdiI GCCGGC 1 cut(s) 99
PdmI GAANNNNTTC 1 cut(s) 22
PfeI GAWTC 7 cut(s) 23, 92, 249, 416, 581, 705, 1102
PflFI GACNNNGTC 1 cut(s) 1031
PkrI GCNGC 5 cut(s) 108, 337, 519, 591, 701
PleI GAGTC 4 cut(s) 131, 193, 257, 601
PpsI GAGTC 4 cut(s) 131, 193, 257, 601
PpuMI RGGWCCY 1 cut(s) 622
Psp5II RGGWCCY 1 cut(s) 622
Psp6I CCWGG 1 cut(s) 289
PspGI CCWGG 1 cut(s) 289
PspN4I GGNNCC 2 cut(s) 624, 953
PspPI GGNCC 2 cut(s) 58, 622
PspPPI RGGWCCY 1 cut(s) 622
PsuI RGATCY 1 cut(s) 386
PsyI GACNNNGTC 1 cut(s) 1031
RsaI GTAC 2 cut(s) 923, 1180
RsaNI GTAC 2 cut(s) 922, 1179
RseI CAYNNNNRTG 3 cut(s) 686, 875, 1137
SalI GTCGAC 1 cut(s) 1025
SaqAI TTAA 3 cut(s) 191, 299, 1008
SatI GCNGC 5 cut(s) 107, 336, 518, 590, 700
Sau3AI GATC 6 cut(s) 386, 651, 739, 813, 817, 966
Sau96I GGNCC 2 cut(s) 58, 622
ScaI AGTACT 1 cut(s) 923
SchI GAGTC 4 cut(s) 132, 194, 257, 601
ScrFI CCNGG 3 cut(s) 291, 626, 1041
SduI GDGCHC 1 cut(s) 1167
SfaNI GCATC 5 cut(s) 335, 504, 517, 551, 788
SfuI TTCGAA 1 cut(s) 16
SinI GGWCC 2 cut(s) 58, 622
SmiMI CAYNNNNRTG 3 cut(s) 686, 875, 1137
SmlI CTYRAG 2 cut(s) 735, 1097
SmoI CTYRAG 2 cut(s) 735, 1097
SsiI CCGC 4 cut(s) 106, 589, 700, 783
SspI AATATT 2 cut(s) 218, 1072
SspMI CTAG 2 cut(s) 356, 425
StyD4I CCNGG 3 cut(s) 289, 624, 1039
StyI CCWWGG 1 cut(s) 390
TaaI ACNGT 3 cut(s) 670, 874, 985
TaqI TCGA 4 cut(s) 16, 329, 816, 1026
TatI WGTACW 2 cut(s) 921, 1178
TauI GCSGC 3 cut(s) 109, 592, 702
TfiI GAWTC 7 cut(s) 23, 92, 249, 416, 581, 705, 1102
Tru1I TTAA 3 cut(s) 191, 299, 1008
Tru9I TTAA 3 cut(s) 191, 299, 1008
TscAI CASTG 3 cut(s) 87, 877, 990
TseFI GTSAC 3 cut(s) 4, 76, 891
TseI GCWGC 2 cut(s) 335, 517
Tsp45I GTSAC 3 cut(s) 4, 76, 891
TspDTI ATGAA 4 cut(s) 270, 433, 670, 1121
TspGWI ACGGA 1 cut(s) 56
TspRI CASTG 3 cut(s) 87, 877, 990
Tth111I GACNNNGTC 1 cut(s) 1031
VpaK11BI GGWCC 2 cut(s) 58, 622
XapI RAATTY 2 cut(s) 12, 570
XmiI GTMKAC 2 cut(s) 848, 1026
XmnI GAANNNNTTC 1 cut(s) 22
XspI CTAG 2 cut(s) 356, 425
ZrmI AGTACT 1 cut(s) 923
Zsp2I ATGCAT 1 cut(s) 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.