RLG00000032756

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0001629
Physical Location & Seq
Reverse (-)
18679224 .. 18681184
1961 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032756

Sequence Viewer

Length: 1209 bp
ATGGTGACAGAACTTCGAACGGATTCTTTGGATTACCGCACAGAGCTGTTGTCGCCGGACCTGACAAGAGAGACTGTGACACTGCCAAGTGAGCCGGCGTGGCGGCTTAATATGGATGGCTTCAAGCTTCCAGCTGAGAGAAACAACACGGATCCTTTCTTTGGCGTTGGCTCTTTTGTTCAGTCATTAAGGACGAAAAGAAAGGTTGCACAGTATTACAGGAGGCAGAACAAACTTCTCAAAGGTTTCAATGAATTAGACTCATTTTCTGAAATGGGTTTTTGGCCTGGAAGTTTAACAAAGGATGAGATGACACAGCTTGAAAGGAGTGAAAGGATAGCAATATATGCATCCAATGCGGCTAATTTGGTGCTTTTCCTAGCAAAAGTGTATGCTTCTATTCAGAGCAGATCATTGGCAGTAATAGCTTCAACTTTGGACTCCCTCTTAGACCTCTTGTCAGGGTTCATCCTTTGGTTTACAGCTTCTGCAATGAGAAAACCAAATCAGTATCACTACCCGATTGGCAAGACTCGGATGCAACCTGTGGTAAGCATTAACCAACTTCAGGAGACATCAGTGACAATCTATAAGGAGTCATCAGTGATCATAAGCCGACAACTTGTCATGAAGACTCAACCTGATAAGGACCCAGAGAAAGAGAAATGGATGATAGGGATAATGGTCTCTGTCACAGTAGTCAAGATTGTTCTAATGGTATACTGTCGAAGATTCAATAACGAAATTGTTCGAGCATATGCTCAAGATCATCTATTTGATGTCATCACTAATGCAATTGGTCTTGCATCAGCAGTGTTAGCCGTCAGGTTTTACTGGTGGATCGATCCAATCGGAGCTATCATTATTGCTTTGTATACAATGGGGAACTGGGCAAAAACAGTGATGGACAATGTGTGGTCACTAATTGGGAAGACAGCACCAGCAGAGTACTTGGCCAAGTTAACATATCTAATTTGGAACCATGACAAGGAGATCAAGCACATTGAGACAGTGAGAGCATATACCTTTGGTGTCAACTATTTTGTAGAGGTTCATATAGTCTTACCTGGGGAGATGTCTCTCAGTGATGCACATAATATCGGAGAGGCACTCCAAGAAAAGCTTGAGAATCTTCCTGAGGTTGAACGAGCTTTTGTTCATGTTGATGTTGACATTACTCACAAGCCAGAGCACAAGCCAAAGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux ZT_dimer

Protein Analysis

403

Amino Acids

45.95

Weight (kDa)

7.14

Isoelectric Point (pI)

41.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_efflux PF01545 117 - 308 3.6e-26 Cation efflux transmembrane domain
ZT_dimer PF16916 321 - 389 3.9e-10 Cation efflux protein, cytoplasmic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 720, 875
AciI CCGC 3 cut(s) 37, 103, 359
AclWI GGATC 4 cut(s) 146, 159, 839, 848
AcoI YGGCCR 1 cut(s) 954
AcuI CTGAAG 1 cut(s) 551
AfaI GTAC 1 cut(s) 950
AfiI CCNNNNNNNGG 3 cut(s) 161, 568, 988
AgsI TTSAA 6 cut(s) 124, 250, 323, 432, 736, 1145
AhdI GACNNNNNGTC 2 cut(s) 457, 623
AjnI CCWGG 2 cut(s) 286, 1066
AluBI AGCT 9 cut(s) 46, 127, 134, 319, 428, 485, 857, 1123, 1151
AluI AGCT 9 cut(s) 46, 127, 134, 319, 428, 485, 857, 1123, 1151
Alw21I GWGCWC 1 cut(s) 1194
Alw26I GTCTC 5 cut(s) 65, 566, 691, 1001, 1083
AlwI GGATC 4 cut(s) 146, 159, 839, 848
AlwNI CAGNNNCTG 1 cut(s) 488
AoxI GGCC 2 cut(s) 284, 954
Asp700I GAANNNNTTC 2 cut(s) 22, 747
AspS9I GGNCC 2 cut(s) 58, 649
AsuHPI GGTGA 1 cut(s) 16
AsuII TTCGAA 1 cut(s) 16
AvaII GGWCC 2 cut(s) 58, 649
AxyI CCTNAGG 1 cut(s) 1137
BalI TGGCCA 1 cut(s) 956
BamHI GGATCC 1 cut(s) 151
BbsI GAAGAC 2 cut(s) 638, 938
Bbv12I GWGCWC 1 cut(s) 1194
BccI CCATC 2 cut(s) 110, 898
BceAI ACGGC 1 cut(s) 806
BciT130I CCWGG 2 cut(s) 288, 1068
BclI TGATCA 1 cut(s) 606
BcoDI GTCTC 5 cut(s) 65, 566, 691, 1001, 1083
BfaI CTAG 1 cut(s) 380
BglI GCCNNNNNGGC 1 cut(s) 100
BisI GCNGC 2 cut(s) 104, 360
BlsI GCNGC 2 cut(s) 105, 361
BmcAI AGTACT 1 cut(s) 950
Bme1390I CCNGG 2 cut(s) 288, 1068
Bme18I GGWCC 2 cut(s) 58, 649
BmeRI GACNNNNNGTC 2 cut(s) 457, 623
BmgT120I GGNCC 2 cut(s) 58, 649
BmiI GGNNCC 3 cut(s) 153, 651, 980
BmrFI CCNGG 2 cut(s) 288, 1068
BmrI ACTGGG 1 cut(s) 898
BmsI GCATC 4 cut(s) 359, 528, 815, 1078
BmuI ACTGGG 1 cut(s) 898
BpiI GAAGAC 2 cut(s) 638, 938
BplI GAGNNNNNCTC 2 cut(s) 1095, 1127
Bpu14I TTCGAA 1 cut(s) 16
BpuEI CTTGAG 2 cut(s) 747, 1145
Bsa29I ATCGAT 1 cut(s) 843
BsaI GGTCTC 1 cut(s) 691
BsaJI CCNNGG 1 cut(s) 1067
Bsc4I CCNNNNNNNGG 3 cut(s) 161, 568, 988
Bse118I RCCGGY 1 cut(s) 94
Bse1I ACTGG 2 cut(s) 839, 893
Bse21I CCTNAGG 1 cut(s) 1137
Bse3DI GCAATG 1 cut(s) 498
BseBI CCWGG 2 cut(s) 288, 1068
BseCI ATCGAT 1 cut(s) 843
BseDI CCNNGG 1 cut(s) 1067
BseGI GGATG 6 cut(s) 121, 310, 350, 468, 543, 675
BseLI CCNNNNNNNGG 3 cut(s) 161, 568, 988
BseMI GCAATG 1 cut(s) 498
BseMII CTCAG 3 cut(s) 126, 1096, 1128
BseNI ACTGG 2 cut(s) 839, 893
BshFI GGCC 2 cut(s) 286, 956
BshVI ATCGAT 1 cut(s) 843
BsiHKAI GWGCWC 1 cut(s) 1194
BsiSI CCGG 2 cut(s) 56, 95
BslI CCNNNNNNNGG 3 cut(s) 161, 568, 988
BsmAI GTCTC 5 cut(s) 65, 566, 691, 1001, 1083
BsnI GGCC 2 cut(s) 286, 956
Bso31I GGTCTC 1 cut(s) 691
Bsp119I TTCGAA 1 cut(s) 16
Bsp1286I GDGCHC 1 cut(s) 1194
Bsp143I GATC 7 cut(s) 151, 410, 606, 766, 840, 844, 993
BspACI CCGC 3 cut(s) 37, 103, 359
BspANI GGCC 2 cut(s) 286, 956
BspCNI CTCAG 3 cut(s) 127, 1095, 1129
BspDI ATCGAT 1 cut(s) 843
BspHI TCATGA 1 cut(s) 627
BspLI GGNNCC 3 cut(s) 153, 651, 980
BspPI GGATC 4 cut(s) 146, 159, 839, 848
BspT104I TTCGAA 1 cut(s) 16
BspTNI GGTCTC 1 cut(s) 691
BsrDI GCAATG 1 cut(s) 498
BsrFI RCCGGY 1 cut(s) 94
BsrI ACTGG 2 cut(s) 839, 893
BssAI RCCGGY 1 cut(s) 94
BssECI CCNNGG 1 cut(s) 1067
BssMI GATC 7 cut(s) 151, 410, 606, 766, 840, 844, 993
BssNAI GTATAC 2 cut(s) 721, 876
Bst1107I GTATAC 2 cut(s) 721, 876
Bst2UI CCWGG 2 cut(s) 288, 1068
Bst4CI ACNGT 6 cut(s) 76, 213, 697, 725, 901, 1012
BstAPI GCANNNNNTGC 2 cut(s) 347, 356
BstBI TTCGAA 1 cut(s) 16
BstC8I GCNNGC 1 cut(s) 96
BstDEI CTNAG 4 cut(s) 135, 448, 1082, 1137
BstF5I GGATG 6 cut(s) 121, 310, 350, 468, 543, 675
BstKTI GATC 7 cut(s) 154, 413, 609, 769, 843, 847, 996
BstMAI GTCTC 5 cut(s) 65, 566, 691, 1001, 1083
BstMBI GATC 7 cut(s) 151, 410, 606, 766, 840, 844, 993
BstMWI GCNNNNNNNGC 7 cut(s) 52, 91, 100, 347, 356, 425, 818
BstNI CCWGG 2 cut(s) 288, 1068
BstSCI CCNGG 2 cut(s) 286, 1066
BstV2I GAAGAC 2 cut(s) 638, 938
BstX2I RGATCY 1 cut(s) 151
BstYI RGATCY 1 cut(s) 151
BstZ17I GTATAC 2 cut(s) 721, 876
Bsu15I ATCGAT 1 cut(s) 843
Bsu36I CCTNAGG 1 cut(s) 1137
BsuRI GGCC 2 cut(s) 286, 956
BsuTUI ATCGAT 1 cut(s) 843
BtsCI GGATG 6 cut(s) 121, 310, 350, 468, 543, 675
BtsI GCAGTG 2 cut(s) 80, 819
BtsIMutI CAGTG 7 cut(s) 80, 585, 609, 819, 906, 1017, 1090
Cac8I GCNNGC 1 cut(s) 96
CaiI CAGNNNCTG 1 cut(s) 488
CciI TCATGA 1 cut(s) 627
Cfr10I RCCGGY 1 cut(s) 94
Cfr13I GGNCC 2 cut(s) 58, 649
ClaI ATCGAT 1 cut(s) 843
Csp6I GTAC 1 cut(s) 949
CviAII CATG 3 cut(s) 628, 983, 1160
CviQI GTAC 1 cut(s) 949
DdeI CTNAG 4 cut(s) 135, 448, 1082, 1137
DpnI GATC 7 cut(s) 153, 412, 608, 768, 842, 846, 995
DpnII GATC 7 cut(s) 151, 410, 606, 766, 840, 844, 993
DriI GACNNNNNGTC 2 cut(s) 457, 623
EaeI YGGCCR 1 cut(s) 954
Eam1105I GACNNNNNGTC 2 cut(s) 457, 623
Eco31I GGTCTC 1 cut(s) 691
Eco47I GGWCC 2 cut(s) 58, 649
Eco57I CTGAAG 1 cut(s) 551
Eco81I CCTNAGG 1 cut(s) 1137
EcoO109I RGGNCCY 1 cut(s) 649
EcoRII CCWGG 2 cut(s) 286, 1066
EcoT22I ATGCAT 1 cut(s) 352
FaeI CATG 3 cut(s) 631, 986, 1163
FalI AAGNNNNNCTT 2 cut(s) 1107, 1139
FatI CATG 3 cut(s) 627, 982, 1159
FauNDI CATATG 1 cut(s) 757
FbaI TGATCA 1 cut(s) 606
FblI GTMKAC 2 cut(s) 720, 875
Fnu4HI GCNGC 2 cut(s) 104, 360
FokI GGATG 6 cut(s) 128, 317, 337, 455, 550, 682
Fsp4HI GCNGC 2 cut(s) 104, 360
FspBI CTAG 1 cut(s) 380
GluI GCNGC 2 cut(s) 104, 360
HaeIII GGCC 2 cut(s) 286, 956
HapII CCGG 2 cut(s) 56, 95
Hin1II CATG 3 cut(s) 631, 986, 1163
HincII GTYRAC 3 cut(s) 963, 1036, 1171
HindII GTYRAC 3 cut(s) 963, 1036, 1171
HindIII AAGCTT 2 cut(s) 125, 1121
HinfI GANTC 8 cut(s) 23, 260, 440, 532, 596, 634, 732, 1129
HpaI GTTAAC 1 cut(s) 963
HpaII CCGG 2 cut(s) 56, 95
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 6 cut(s) 480, 721, 876, 963, 1036, 1171
Hpy188I TCNGA 5 cut(s) 271, 405, 537, 854, 1103
Hpy188III TCNNGA 5 cut(s) 569, 628, 703, 764, 1136
Hpy8I GTNNAC 6 cut(s) 480, 721, 876, 963, 1036, 1171
HpyCH4III ACNGT 6 cut(s) 76, 213, 697, 725, 901, 1012
HpyCH4V TGCA 7 cut(s) 209, 350, 491, 541, 794, 806, 1091
HpyF10VI GCNNNNNNNGC 7 cut(s) 52, 91, 100, 347, 356, 425, 818
HpyF3I CTNAG 4 cut(s) 135, 448, 1082, 1137
Hsp92II CATG 3 cut(s) 631, 986, 1163
KroI GCCGGC 1 cut(s) 94
KroNI GCCGGC 1 cut(s) 96
Ksp22I TGATCA 1 cut(s) 606
KspAI GTTAAC 1 cut(s) 963
Kzo9I GATC 7 cut(s) 151, 410, 606, 766, 840, 844, 993
LmnI GCTCC 1 cut(s) 854
LweI GCATC 4 cut(s) 359, 528, 815, 1078
MaeI CTAG 1 cut(s) 380
MaeIII GTNAC 5 cut(s) 4, 76, 580, 691, 918
MalI GATC 7 cut(s) 153, 412, 608, 768, 842, 846, 995
MboI GATC 7 cut(s) 151, 410, 606, 766, 840, 844, 993
MboII GAAGA 4 cut(s) 643, 741, 943, 1124
MfeI CAATTG 1 cut(s) 795
MflI RGATCY 1 cut(s) 151
MhlI GDGCHC 1 cut(s) 1194
MlsI TGGCCA 1 cut(s) 956
MluCI AATT 6 cut(s) 254, 364, 744, 795, 924, 972
MluNI TGGCCA 1 cut(s) 956
MlyI GAGTC 5 cut(s) 254, 434, 526, 605, 628
MnlI CCTC 6 cut(s) 216, 455, 464, 1042, 1099, 1132
Mox20I TGGCCA 1 cut(s) 956
Mph1103I ATGCAT 1 cut(s) 352
MroNI GCCGGC 1 cut(s) 94
MroXI GAANNNNTTC 2 cut(s) 22, 747
MscI TGGCCA 1 cut(s) 956
MseI TTAA 5 cut(s) 108, 188, 296, 558, 962
MslI CAYNNNNRTG 1 cut(s) 1164
Msp20I TGGCCA 1 cut(s) 956
MspA1I CMGCKG 1 cut(s) 134
MspI CCGG 2 cut(s) 56, 95
MspR9I CCNGG 2 cut(s) 288, 1068
MunI CAATTG 1 cut(s) 795
MvaI CCWGG 2 cut(s) 288, 1068
MwoI GCNNNNNNNGC 7 cut(s) 52, 91, 100, 347, 356, 425, 818
NaeI GCCGGC 1 cut(s) 96
NdeI CATATG 1 cut(s) 757
NdeII GATC 7 cut(s) 151, 410, 606, 766, 840, 844, 993
NgoMIV GCCGGC 1 cut(s) 94
NlaIII CATG 3 cut(s) 631, 986, 1163
NlaIV GGNNCC 3 cut(s) 153, 651, 980
NmuCI GTSAC 5 cut(s) 4, 76, 580, 691, 918
NsiI ATGCAT 1 cut(s) 352
NspV TTCGAA 1 cut(s) 16
PagI TCATGA 1 cut(s) 627
PdiI GCCGGC 1 cut(s) 96
PdmI GAANNNNTTC 2 cut(s) 22, 747
PfeI GAWTC 3 cut(s) 23, 732, 1129
PkrI GCNGC 2 cut(s) 105, 361
PleI GAGTC 5 cut(s) 254, 434, 526, 604, 628
PpsI GAGTC 5 cut(s) 254, 434, 526, 604, 628
PpuMI RGGWCCY 1 cut(s) 649
Psp5II RGGWCCY 1 cut(s) 649
Psp6I CCWGG 2 cut(s) 286, 1066
PspGI CCWGG 2 cut(s) 286, 1066
PspN4I GGNNCC 3 cut(s) 153, 651, 980
PspPI GGNCC 2 cut(s) 58, 649
PspPPI RGGWCCY 1 cut(s) 649
PstNI CAGNNNCTG 1 cut(s) 488
PsuI RGATCY 1 cut(s) 151
PvuII CAGCTG 1 cut(s) 134
RsaI GTAC 1 cut(s) 950
RsaNI GTAC 1 cut(s) 949
RseI CAYNNNNRTG 1 cut(s) 1164
SaqAI TTAA 5 cut(s) 108, 188, 296, 558, 962
SatI GCNGC 2 cut(s) 104, 360
Sau3AI GATC 7 cut(s) 151, 410, 606, 766, 840, 844, 993
Sau96I GGNCC 2 cut(s) 58, 649
ScaI AGTACT 1 cut(s) 950
SchI GAGTC 5 cut(s) 254, 434, 526, 605, 628
ScrFI CCNGG 2 cut(s) 288, 1068
SduI GDGCHC 1 cut(s) 1194
SfaNI GCATC 4 cut(s) 359, 528, 815, 1078
SfuI TTCGAA 1 cut(s) 16
SinI GGWCC 2 cut(s) 58, 649
SmiMI CAYNNNNRTG 1 cut(s) 1164
SmlI CTYRAG 2 cut(s) 762, 1124
SmoI CTYRAG 2 cut(s) 762, 1124
Sse9I AATT 6 cut(s) 254, 364, 744, 795, 924, 972
SsiI CCGC 3 cut(s) 37, 103, 359
SspMI CTAG 1 cut(s) 380
StyD4I CCNGG 2 cut(s) 286, 1066
TaaI ACNGT 6 cut(s) 76, 213, 697, 725, 901, 1012
TaqI TCGA 4 cut(s) 16, 727, 751, 843
TasI AATT 6 cut(s) 254, 364, 744, 795, 924, 972
TatI WGTACW 1 cut(s) 948
TauI GCSGC 2 cut(s) 106, 362
TfiI GAWTC 3 cut(s) 23, 732, 1129
Tru1I TTAA 5 cut(s) 108, 188, 296, 558, 962
Tru9I TTAA 5 cut(s) 108, 188, 296, 558, 962
TscAI CASTG 7 cut(s) 87, 585, 609, 819, 906, 1017, 1090
TseFI GTSAC 5 cut(s) 4, 76, 580, 691, 918
Tsp45I GTSAC 5 cut(s) 4, 76, 580, 691, 918
TspDTI ATGAA 5 cut(s) 267, 457, 644, 1043, 1148
TspGWI ACGGA 2 cut(s) 35, 164
TspRI CASTG 7 cut(s) 87, 585, 609, 819, 906, 1017, 1090
VpaK11BI GGWCC 2 cut(s) 58, 649
XmiI GTMKAC 2 cut(s) 720, 875
XmnI GAANNNNTTC 2 cut(s) 22, 747
XspI CTAG 1 cut(s) 380
ZrmI AGTACT 1 cut(s) 950
Zsp2I ATGCAT 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.