Rmu_sc0011761.1_g000003

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0011761.1
OG0001629
Physical Location & Seq
Reverse (-)
22107 .. 24323
2217 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0011761.1_g000003.1.cds

Sequence Viewer

Length: 1392 bp
atggtgacagaaattcgaacagattctttggactaccggacggagttgttgtcgccggacctgacagcagggagtgtgacactgccaagtgattcgccggcatggcggctcaatatggatggagtcaagcttccagctgaaagaaacaatatgagttcttgttttggcataggctcttatgttgagtcattaaggaggcaaaagaaagttgcagaatattacaagaggcagaataaacttctcaaaggattcaatgaattagactcattttctgaattgggttcttggcctggaagtttaacagaggatgagaagaaagaacttgcaaccagcgaaaggatagcaatatatgcatcgaatgcggctaacttggtgctgttcctagcaaaagtgtatgcttcagttgagagcagatccttggcagttatagcttcaactttggattccctcctagacctcttgtcagggttcatcctttggtttacagctcatgcaatgagaaaaccaaatcaatatagctacccgattggcaagcatcggatgcagcccgtgggaattgttgtttttgcatcagtaatggcaactcttggactacaaattttgtttgaatcagggcggcaacttgtcagtaagactcaacctgataaggacccggagaaagaaaaatggatgatagggatcatggtctctgccacagtaatcaagttcattctaatggcatactgccgcagattcaaaaacgagattgttcaagcatatgctcaagatcatctatttgatgtcattactaatggaattggtcttgcatccgcagttttagcaatcaggttttattggtggatcgatcctgttggagccatcattattgctttgtatacaattgggaattgggcaaacactgtgatggagaatgtgtggtcactaattggcaagacagcaccagcagagtacttagccaagttgacatatctaatttggaaccatgacaagcaagttttagcaatcaggttttattggtggatcgatcctgttggagccatcattattgctttgtatacaattgggaattgggcaaacactgtgatggagaatgtgtggtcactaattggcaagacagcaccagcagagtacttagccaagttgacatatctaatttggaaccatgacaagcagatcaagcacattgacacagtgagagcatatacatttggtattaactactttgtggaggtcgacatagtcttacccggagatatgtccctttgtgaagcacataatattggagaggcactccaagaaaagcttgagaatcttcctgaggttgaacgagcttttgttcatgtggatgttgattttactcacaagccagagcacaagccaaagagtacataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux ZT_dimer

Protein Analysis

463

Amino Acids

52.37

Weight (kDa)

6.24

Isoelectric Point (pI)

35.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 875, 1055, 1233
AciI CCGC 5 cut(s) 106, 362, 616, 727, 810
AclWI GGATC 6 cut(s) 408, 686, 839, 848, 1019, 1028
AcsI RAATTY 2 cut(s) 12, 597
AcuI CTGAAG 1 cut(s) 384
AfaI GTAC 3 cut(s) 950, 1130, 1387
AfiI CCNNNNNNNGG 1 cut(s) 336
AgsI TTSAA 6 cut(s) 253, 435, 608, 736, 752, 1325
AhdI GACNNNNNGTC 1 cut(s) 460
AjnI CCWGG 1 cut(s) 289
AluBI AGCT 7 cut(s) 130, 137, 431, 488, 519, 1303, 1331
AluI AGCT 7 cut(s) 130, 137, 431, 488, 519, 1303, 1331
Alw21I GWGCWC 1 cut(s) 1374
Alw26I GTCTC 1 cut(s) 691
AlwI GGATC 6 cut(s) 408, 686, 839, 848, 1019, 1028
AoxI GGCC 1 cut(s) 287
ApeKI GCWGC 1 cut(s) 544
ApoI RAATTY 2 cut(s) 12, 597
Asp700I GAANNNNTTC 1 cut(s) 22
AspS9I GGNCC 2 cut(s) 58, 649
AsuC2I CCSGG 2 cut(s) 653, 1248
AsuHPI GGTGA 1 cut(s) 16
AsuII TTCGAA 1 cut(s) 16
AvaII GGWCC 2 cut(s) 58, 649
AxyI CCTNAGG 1 cut(s) 1317
Bbv12I GWGCWC 1 cut(s) 1374
BbvI GCAGC 1 cut(s) 556
BccI CCATC 5 cut(s) 113, 866, 898, 1046, 1078
BciT130I CCWGG 1 cut(s) 291
BcnI CCSGG 2 cut(s) 653, 1248
BcoDI GTCTC 1 cut(s) 691
BfaI CTAG 2 cut(s) 383, 452
BglI GCCNNNNNGGC 1 cut(s) 103
BisI GCNGC 5 cut(s) 107, 363, 545, 617, 727
BlsI GCNGC 5 cut(s) 108, 364, 546, 618, 728
BmcAI AGTACT 2 cut(s) 950, 1130
Bme1390I CCNGG 3 cut(s) 291, 653, 1248
Bme18I GGWCC 2 cut(s) 58, 649
BmeRI GACNNNNNGTC 1 cut(s) 460
BmgT120I GGNCC 2 cut(s) 58, 649
BmiI GGNNCC 5 cut(s) 651, 856, 980, 1036, 1160
BmrFI CCNGG 3 cut(s) 291, 653, 1248
BmsI GCATC 5 cut(s) 362, 531, 544, 578, 815
BplI GAGNNNNNCTC 2 cut(s) 1275, 1307
Bpu14I TTCGAA 1 cut(s) 16
BpuEI CTTGAG 2 cut(s) 747, 1325
BpuMI CCSGG 2 cut(s) 653, 1248
Bsa29I ATCGAT 2 cut(s) 843, 1023
BsaBI GATNNNNATC 1 cut(s) 677
BsaI GGTCTC 1 cut(s) 691
BsaJI CCNNGG 2 cut(s) 417, 549
BsaWI WCCGGW 1 cut(s) 36
BsaXI ACNNNNNCTCC 6 cut(s) 35, 65, 899, 929, 1079, 1109
Bsc4I CCNNNNNNNGG 1 cut(s) 336
Bse118I RCCGGY 1 cut(s) 97
Bse21I CCTNAGG 1 cut(s) 1317
Bse3DI GCAATG 1 cut(s) 501
Bse8I GATNNNNATC 1 cut(s) 677
BseBI CCWGG 1 cut(s) 291
BseCI ATCGAT 2 cut(s) 843, 1023
BseDI CCNNGG 2 cut(s) 417, 549
BseGI GGATG 7 cut(s) 124, 313, 471, 546, 675, 806, 1351
BseJI GATNNNNATC 1 cut(s) 677
BseLI CCNNNNNNNGG 1 cut(s) 336
BseMI GCAATG 1 cut(s) 501
BseMII CTCAG 1 cut(s) 1308
BseXI GCAGC 1 cut(s) 556
BshFI GGCC 1 cut(s) 289
BshVI ATCGAT 2 cut(s) 843, 1023
BsiHKAI GWGCWC 1 cut(s) 1374
BsiSI CCGG 5 cut(s) 37, 56, 98, 653, 1248
BslFI GGGAC 1 cut(s) 1243
BslI CCNNNNNNNGG 1 cut(s) 336
BsmAI GTCTC 1 cut(s) 691
BsmFI GGGAC 1 cut(s) 1243
BsmI GAATGC 1 cut(s) 364
BsnI GGCC 1 cut(s) 289
Bso31I GGTCTC 1 cut(s) 691
Bsp119I TTCGAA 1 cut(s) 16
Bsp1286I GDGCHC 1 cut(s) 1374
Bsp143I GATC 8 cut(s) 413, 678, 766, 840, 844, 1020, 1024, 1173
BspACI CCGC 5 cut(s) 106, 362, 616, 727, 810
BspANI GGCC 1 cut(s) 289
BspCNI CTCAG 1 cut(s) 1309
BspDI ATCGAT 2 cut(s) 843, 1023
BspLI GGNNCC 5 cut(s) 651, 856, 980, 1036, 1160
BspPI GGATC 6 cut(s) 408, 686, 839, 848, 1019, 1028
BspT104I TTCGAA 1 cut(s) 16
BspTNI GGTCTC 1 cut(s) 691
BsrDI GCAATG 1 cut(s) 501
BsrFI RCCGGY 1 cut(s) 97
BssAI RCCGGY 1 cut(s) 97
BssECI CCNNGG 2 cut(s) 417, 549
BssMI GATC 8 cut(s) 413, 678, 766, 840, 844, 1020, 1024, 1173
BssNAI GTATAC 2 cut(s) 876, 1056
BssT1I CCWWGG 1 cut(s) 417
Bst1107I GTATAC 2 cut(s) 876, 1056
Bst2UI CCWGG 1 cut(s) 291
Bst4CI ACNGT 4 cut(s) 697, 901, 1081, 1192
BstAPI GCANNNNNTGC 3 cut(s) 350, 359, 541
BstBI TTCGAA 1 cut(s) 16
BstC8I GCNNGC 2 cut(s) 99, 533
BstDEI CTNAG 3 cut(s) 952, 1132, 1317
BstDSI CCRYGG 1 cut(s) 549
BstF5I GGATG 7 cut(s) 124, 313, 471, 546, 675, 806, 1351
BstKTI GATC 8 cut(s) 416, 681, 769, 843, 847, 1023, 1027, 1176
BstMAI GTCTC 1 cut(s) 691
BstMBI GATC 8 cut(s) 413, 678, 766, 840, 844, 1020, 1024, 1173
BstMWI GCNNNNNNNGC 7 cut(s) 103, 350, 359, 428, 541, 818, 1177
BstNI CCWGG 1 cut(s) 291
BstSCI CCNGG 3 cut(s) 289, 651, 1246
BstV1I GCAGC 1 cut(s) 556
BstX2I RGATCY 1 cut(s) 413
BstYI RGATCY 1 cut(s) 413
BstZ17I GTATAC 2 cut(s) 876, 1056
Bsu15I ATCGAT 2 cut(s) 843, 1023
Bsu36I CCTNAGG 1 cut(s) 1317
BsuRI GGCC 1 cut(s) 289
BsuTUI ATCGAT 2 cut(s) 843, 1023
BtgI CCRYGG 1 cut(s) 549
BtsCI GGATG 7 cut(s) 124, 313, 471, 546, 675, 806, 1351
BtsI GCAGTG 1 cut(s) 80
BtsIMutI CAGTG 4 cut(s) 80, 897, 1077, 1197
Cac8I GCNNGC 2 cut(s) 99, 533
Cfr10I RCCGGY 1 cut(s) 97
Cfr13I GGNCC 2 cut(s) 58, 649
ClaI ATCGAT 2 cut(s) 843, 1023
Csp6I GTAC 3 cut(s) 949, 1129, 1386
CviAII CATG 6 cut(s) 102, 491, 682, 983, 1163, 1340
CviQI GTAC 3 cut(s) 949, 1129, 1386
DdeI CTNAG 3 cut(s) 952, 1132, 1317
DpnI GATC 8 cut(s) 415, 680, 768, 842, 846, 1022, 1026, 1175
DpnII GATC 8 cut(s) 413, 678, 766, 840, 844, 1020, 1024, 1173
DriI GACNNNNNGTC 1 cut(s) 460
Eam1105I GACNNNNNGTC 1 cut(s) 460
Eco130I CCWWGG 1 cut(s) 417
Eco31I GGTCTC 1 cut(s) 691
Eco47I GGWCC 2 cut(s) 58, 649
Eco57I CTGAAG 1 cut(s) 384
Eco81I CCTNAGG 1 cut(s) 1317
EcoO109I RGGNCCY 1 cut(s) 649
EcoRII CCWGG 1 cut(s) 289
EcoT14I CCWWGG 1 cut(s) 417
EcoT22I ATGCAT 1 cut(s) 355
ErhI CCWWGG 1 cut(s) 417
FaeI CATG 6 cut(s) 105, 494, 685, 986, 1166, 1343
FalI AAGNNNNNCTT 2 cut(s) 1287, 1319
FaqI GGGAC 1 cut(s) 1243
FatI CATG 6 cut(s) 101, 490, 681, 982, 1162, 1339
FauNDI CATATG 1 cut(s) 757
FblI GTMKAC 3 cut(s) 875, 1055, 1233
Fnu4HI GCNGC 5 cut(s) 107, 363, 545, 617, 727
FokI GGATG 7 cut(s) 131, 320, 458, 553, 682, 793, 1358
Fsp4HI GCNGC 5 cut(s) 107, 363, 545, 617, 727
FspBI CTAG 2 cut(s) 383, 452
GluI GCNGC 5 cut(s) 107, 363, 545, 617, 727
HaeIII GGCC 1 cut(s) 289
HapII CCGG 5 cut(s) 37, 56, 98, 653, 1248
Hin1II CATG 6 cut(s) 105, 494, 685, 986, 1166, 1343
HincII GTYRAC 3 cut(s) 963, 1143, 1234
HindII GTYRAC 3 cut(s) 963, 1143, 1234
HindIII AAGCTT 2 cut(s) 128, 1301
HpaII CCGG 5 cut(s) 37, 56, 98, 653, 1248
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 6 cut(s) 483, 876, 963, 1056, 1143, 1234
Hpy188I TCNGA 2 cut(s) 274, 540
Hpy188III TCNNGA 2 cut(s) 764, 1316
Hpy8I GTNNAC 6 cut(s) 483, 876, 963, 1056, 1143, 1234
HpyCH4III ACNGT 4 cut(s) 697, 901, 1081, 1192
HpyCH4V TGCA 7 cut(s) 212, 326, 353, 494, 544, 569, 806
HpyF10VI GCNNNNNNNGC 7 cut(s) 103, 350, 359, 428, 541, 818, 1177
HpyF3I CTNAG 3 cut(s) 952, 1132, 1317
Hsp92II CATG 6 cut(s) 105, 494, 685, 986, 1166, 1343
KroI GCCGGC 1 cut(s) 97
KroNI GCCGGC 1 cut(s) 99
Kzo9I GATC 8 cut(s) 413, 678, 766, 840, 844, 1020, 1024, 1173
LmnI GCTCC 2 cut(s) 854, 1034
Lsp1109I GCAGC 1 cut(s) 556
LweI GCATC 5 cut(s) 362, 531, 544, 578, 815
MaeI CTAG 2 cut(s) 383, 452
MaeIII GTNAC 4 cut(s) 4, 76, 918, 1098
MalI GATC 8 cut(s) 415, 680, 768, 842, 846, 1022, 1026, 1175
MboI GATC 8 cut(s) 413, 678, 766, 840, 844, 1020, 1024, 1173
MboII GAAGA 2 cut(s) 325, 1304
MfeI CAATTG 2 cut(s) 879, 1059
MflI RGATCY 1 cut(s) 413
MhlI GDGCHC 1 cut(s) 1374
MlyI GAGTC 4 cut(s) 132, 194, 257, 628
MmeI TCCRAC 2 cut(s) 832, 1012
MnlI CCTC 8 cut(s) 189, 219, 298, 458, 467, 1222, 1279, 1312
Mph1103I ATGCAT 1 cut(s) 355
MroNI GCCGGC 1 cut(s) 97
MroXI GAANNNNTTC 1 cut(s) 22
MseI TTAA 3 cut(s) 191, 299, 1215
MslI CAYNNNNRTG 4 cut(s) 713, 902, 1082, 1344
MspA1I CMGCKG 1 cut(s) 137
MspI CCGG 5 cut(s) 37, 56, 98, 653, 1248
MspR9I CCNGG 3 cut(s) 291, 653, 1248
MunI CAATTG 2 cut(s) 879, 1059
Mva1269I GAATGC 1 cut(s) 364
MvaI CCWGG 1 cut(s) 291
MwoI GCNNNNNNNGC 7 cut(s) 103, 350, 359, 428, 541, 818, 1177
NaeI GCCGGC 1 cut(s) 99
NciI CCSGG 2 cut(s) 653, 1248
NdeI CATATG 1 cut(s) 757
NdeII GATC 8 cut(s) 413, 678, 766, 840, 844, 1020, 1024, 1173
NgoMIV GCCGGC 1 cut(s) 97
NlaIII CATG 6 cut(s) 105, 494, 685, 986, 1166, 1343
NlaIV GGNNCC 5 cut(s) 651, 856, 980, 1036, 1160
NmuCI GTSAC 4 cut(s) 4, 76, 918, 1098
NsiI ATGCAT 1 cut(s) 355
NspV TTCGAA 1 cut(s) 16
PctI GAATGC 1 cut(s) 364
PdiI GCCGGC 1 cut(s) 99
PdmI GAANNNNTTC 1 cut(s) 22
PfeI GAWTC 7 cut(s) 23, 92, 249, 443, 608, 732, 1309
PflFI GACNNNGTC 1 cut(s) 1238
PkrI GCNGC 5 cut(s) 108, 364, 546, 618, 728
PleI GAGTC 4 cut(s) 131, 193, 257, 628
PpsI GAGTC 4 cut(s) 131, 193, 257, 628
PpuMI RGGWCCY 1 cut(s) 649
Psp5II RGGWCCY 1 cut(s) 649
Psp6I CCWGG 1 cut(s) 289
PspGI CCWGG 1 cut(s) 289
PspN4I GGNNCC 5 cut(s) 651, 856, 980, 1036, 1160
PspPI GGNCC 2 cut(s) 58, 649
PspPPI RGGWCCY 1 cut(s) 649
PsuI RGATCY 1 cut(s) 413
PsyI GACNNNGTC 1 cut(s) 1238
PvuII CAGCTG 1 cut(s) 137
RsaI GTAC 3 cut(s) 950, 1130, 1387
RsaNI GTAC 3 cut(s) 949, 1129, 1386
RseI CAYNNNNRTG 4 cut(s) 713, 902, 1082, 1344
SalI GTCGAC 1 cut(s) 1232
SaqAI TTAA 3 cut(s) 191, 299, 1215
SatI GCNGC 5 cut(s) 107, 363, 545, 617, 727
Sau3AI GATC 8 cut(s) 413, 678, 766, 840, 844, 1020, 1024, 1173
Sau96I GGNCC 2 cut(s) 58, 649
ScaI AGTACT 2 cut(s) 950, 1130
SchI GAGTC 4 cut(s) 132, 194, 257, 628
ScrFI CCNGG 3 cut(s) 291, 653, 1248
SduI GDGCHC 1 cut(s) 1374
SfaNI GCATC 5 cut(s) 362, 531, 544, 578, 815
SfuI TTCGAA 1 cut(s) 16
SinI GGWCC 2 cut(s) 58, 649
SmiMI CAYNNNNRTG 4 cut(s) 713, 902, 1082, 1344
SmlI CTYRAG 2 cut(s) 762, 1304
SmoI CTYRAG 2 cut(s) 762, 1304
SsiI CCGC 5 cut(s) 106, 362, 616, 727, 810
SspI AATATT 2 cut(s) 218, 1279
SspMI CTAG 2 cut(s) 383, 452
StyD4I CCNGG 3 cut(s) 289, 651, 1246
StyI CCWWGG 1 cut(s) 417
TaaI ACNGT 4 cut(s) 697, 901, 1081, 1192
TaqI TCGA 5 cut(s) 16, 356, 843, 1023, 1233
TatI WGTACW 3 cut(s) 948, 1128, 1385
TauI GCSGC 4 cut(s) 109, 365, 619, 729
TfiI GAWTC 7 cut(s) 23, 92, 249, 443, 608, 732, 1309
Tru1I TTAA 3 cut(s) 191, 299, 1215
Tru9I TTAA 3 cut(s) 191, 299, 1215
TscAI CASTG 4 cut(s) 87, 904, 1084, 1197
TseFI GTSAC 4 cut(s) 4, 76, 918, 1098
TseI GCWGC 1 cut(s) 544
Tsp45I GTSAC 4 cut(s) 4, 76, 918, 1098
TspDTI ATGAA 4 cut(s) 270, 460, 697, 1328
TspGWI ACGGA 1 cut(s) 56
TspRI CASTG 4 cut(s) 87, 904, 1084, 1197
Tth111I GACNNNGTC 1 cut(s) 1238
VpaK11BI GGWCC 2 cut(s) 58, 649
XapI RAATTY 2 cut(s) 12, 597
XmiI GTMKAC 3 cut(s) 875, 1055, 1233
XmnI GAANNNNTTC 1 cut(s) 22
XspI CTAG 2 cut(s) 383, 452
ZrmI AGTACT 2 cut(s) 950, 1130
Zsp2I ATGCAT 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.