pycom05g21190

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0001629
Physical Location & Seq
Forward (+)
23879309 .. 23881560
2252 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g21190.4

Sequence Viewer

Length: 969 bp
ATGGTTGCGGATCTTCGAACAGAATCTTCGGATTACCGGACTGAGCTCTTGTCGCCGGACGTGACGGGAGAGACTGTGACAGTGCCAACTGAGGCATCGTGGAGGCTCAACATGGATGGATTCAACCTTCCAGCTGAGAGAAGCATGGATTCTTACTTTGGCCTCGGCTCTTTTGTGAACTCATTAAGGAGGCAGAGAAAAATTGCACAATATTACAAGAGGCAGAATAAACTTCTCGTAGGATTCAACGAGATAGACTCGTTTTCTGAGATGGGTTTTTGGCCTGGAAGTTTAACACAGGGTATTGTTGTTTTTGCATCTGTAATGGCAACTCTTGGACAACAAATATTGTTTGAATCTGGGCGGCAACTTCTCGCGAAGACTCAACCTGATATGGATCCAGTGAAAGAAAAATGGATGATAGGGATAATGGTGTCTGCAACAGTTGTGAAGTTTGTGTTGATGGTGTATTGTCGTAGATTCAAGAACGAAATTGTTCGAGCTTACGCTCGAGATCATCTGTTTGATGTCATCACTAATGGAATTGGTCTTGCAGCCGCAGTTTTAGCAATCAGGTTATACTGGTGGCTTGATCCTGTTGGAGCTATCATTATTGCTTTGTACACAATGGGGAATTGGGCGAAAACAGTGATGGATAATGTGTGGTCACTGATAGGGAAGACAGCACCAGCAGAGTACTTGGCAAAATTGACATATCTGATATGGAACCATGACAAGGAGATCAAGCACATTGAGACGGTGAGAGCATACACTTTTGGCTGCAACTATTTTGTGGAGGCTGACATTGTCTTGCCCGGAGACATGTCTCTCAGCCACGCACATAACATCGGAGAGACGCTTCAGGAATCGATCGAAAACCTTCCTGAGGTAGAACGAGCTTTCGTTCATGTCGACTTTGATACTACTCACAGGCCTGAGCACAACAATGCTAAGTTAGCACCATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux ZT_dimer

Protein Analysis

323

Amino Acids

36.51

Weight (kDa)

5.79

Isoelectric Point (pI)

39.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 912
AccII CGCG 1 cut(s) 377
AciI CCGC 3 cut(s) 8, 364, 558
AclWI GGATC 4 cut(s) 18, 392, 405, 587
AcuI CTGAAG 1 cut(s) 845
AfaI GTAC 2 cut(s) 623, 698
AfiI CCNNNNNNNGG 2 cut(s) 736, 886
AflIII ACRYGT 1 cut(s) 822
AgsI TTSAA 4 cut(s) 124, 247, 356, 484
AjiI CACGTC 1 cut(s) 61
AjnI CCWGG 1 cut(s) 283
AluBI AGCT 5 cut(s) 46, 134, 503, 605, 899
AluI AGCT 5 cut(s) 46, 134, 503, 605, 899
Alw21I GWGCWC 2 cut(s) 48, 942
Alw26I GTCTC 5 cut(s) 65, 749, 813, 831, 848
AlwI GGATC 4 cut(s) 18, 392, 405, 587
Ama87I CYCGRG 1 cut(s) 510
AoxI GGCC 3 cut(s) 160, 281, 932
ApeKI GCWGC 2 cut(s) 554, 780
Asp700I GAANNNNTTC 2 cut(s) 495, 879
AsuC2I CCSGG 1 cut(s) 816
AsuHPI GGTGA 1 cut(s) 772
AsuII TTCGAA 1 cut(s) 16
AvaI CYCGRG 1 cut(s) 510
AxyI CCTNAGG 1 cut(s) 885
BamHI GGATCC 1 cut(s) 397
BanII GRGCYC 1 cut(s) 48
BbsI GAAGAC 2 cut(s) 386, 686
Bbv12I GWGCWC 2 cut(s) 48, 942
BbvI GCAGC 2 cut(s) 566, 767
BccI CCATC 4 cut(s) 110, 265, 457, 646
BciT130I CCWGG 1 cut(s) 285
BcnI CCSGG 1 cut(s) 816
BcoDI GTCTC 5 cut(s) 65, 749, 813, 831, 848
BisI GCNGC 4 cut(s) 365, 555, 558, 781
BlsI GCNGC 4 cut(s) 366, 556, 559, 782
BmcAI AGTACT 1 cut(s) 698
Bme1390I CCNGG 2 cut(s) 285, 816
BmeT110I CYCGRG 1 cut(s) 510
BmgBI CACGTC 1 cut(s) 61
BmiI GGNNCC 2 cut(s) 399, 728
BmrFI CCNGG 2 cut(s) 285, 816
BmsI GCATC 2 cut(s) 104, 326
BpiI GAAGAC 2 cut(s) 386, 686
BplI GAGNNNNNCTC 2 cut(s) 242, 274
Bpu10I CCTNAGC 1 cut(s) 936
Bpu14I TTCGAA 1 cut(s) 16
BpuMI CCSGG 1 cut(s) 816
Bsa29I ATCGAT 1 cut(s) 869
BsaBI GATNNNNATC 1 cut(s) 396
BsaJI CCNNGG 1 cut(s) 163
BsaWI WCCGGW 1 cut(s) 36
BsaXI ACNNNNNCTCC 2 cut(s) 60, 90
Bsc4I CCNNNNNNNGG 2 cut(s) 736, 886
Bse1I ACTGG 2 cut(s) 401, 587
Bse21I CCTNAGG 1 cut(s) 885
Bse8I GATNNNNATC 1 cut(s) 396
BseBI CCWGG 1 cut(s) 285
BseCI ATCGAT 1 cut(s) 869
BseDI CCNNGG 1 cut(s) 163
BseGI GGATG 2 cut(s) 121, 423
BseJI GATNNNNATC 1 cut(s) 396
BseLI CCNNNNNNNGG 2 cut(s) 736, 886
BseMII CTCAG 7 cut(s) 33, 81, 126, 258, 844, 876, 927
BseNI ACTGG 2 cut(s) 401, 587
BseXI GCAGC 2 cut(s) 566, 767
Bsh1236I CGCG 1 cut(s) 377
Bsh1285I CGRYCG 1 cut(s) 873
BshFI GGCC 3 cut(s) 162, 283, 934
BshVI ATCGAT 1 cut(s) 869
BsiEI CGRYCG 1 cut(s) 873
BsiHKAI GWGCWC 2 cut(s) 48, 942
BsiHKCI CYCGRG 1 cut(s) 510
BsiSI CCGG 3 cut(s) 37, 56, 816
BslI CCNNNNNNNGG 2 cut(s) 736, 886
BsmAI GTCTC 5 cut(s) 65, 749, 813, 831, 848
BsmBI CGTCTC 2 cut(s) 749, 848
BsnI GGCC 3 cut(s) 162, 283, 934
BsoBI CYCGRG 1 cut(s) 510
Bsp119I TTCGAA 1 cut(s) 16
Bsp1286I GDGCHC 2 cut(s) 48, 942
Bsp1407I TGTACA 1 cut(s) 621
Bsp143I GATC 6 cut(s) 10, 397, 514, 592, 741, 870
Bsp68I TCGCGA 1 cut(s) 377
BspACI CCGC 3 cut(s) 8, 364, 558
BspANI GGCC 3 cut(s) 162, 283, 934
BspCNI CTCAG 7 cut(s) 34, 82, 127, 259, 843, 877, 928
BspDI ATCGAT 1 cut(s) 869
BspFNI CGCG 1 cut(s) 377
BspLI GGNNCC 2 cut(s) 399, 728
BspPI GGATC 4 cut(s) 18, 392, 405, 587
BspT104I TTCGAA 1 cut(s) 16
BsrGI TGTACA 1 cut(s) 621
BsrI ACTGG 2 cut(s) 401, 587
BssECI CCNNGG 1 cut(s) 163
BssMI GATC 6 cut(s) 10, 397, 514, 592, 741, 870
Bst2UI CCWGG 1 cut(s) 285
Bst4CI ACNGT 5 cut(s) 76, 82, 445, 649, 760
BstAUI TGTACA 1 cut(s) 621
BstBI TTCGAA 1 cut(s) 16
BstDEI CTNAG 8 cut(s) 42, 90, 135, 267, 830, 885, 936, 951
BstENI CCTNNNNNAGG 1 cut(s) 884
BstF5I GGATG 2 cut(s) 121, 423
BstFNI CGCG 1 cut(s) 377
BstKTI GATC 6 cut(s) 13, 400, 517, 595, 744, 873
BstMAI GTCTC 5 cut(s) 65, 749, 813, 831, 848
BstMBI GATC 6 cut(s) 10, 397, 514, 592, 741, 870
BstMCI CGRYCG 1 cut(s) 873
BstMWI GCNNNNNNNGC 3 cut(s) 52, 566, 956
BstNI CCWGG 1 cut(s) 285
BstNSI RCATGY 1 cut(s) 826
BstSCI CCNGG 2 cut(s) 283, 814
BstUI CGCG 1 cut(s) 377
BstV1I GCAGC 2 cut(s) 566, 767
BstV2I GAAGAC 2 cut(s) 386, 686
BstX2I RGATCY 2 cut(s) 10, 397
BstYI RGATCY 2 cut(s) 10, 397
Bsu15I ATCGAT 1 cut(s) 869
Bsu36I CCTNAGG 1 cut(s) 885
BsuRI GGCC 3 cut(s) 162, 283, 934
BsuTUI ATCGAT 1 cut(s) 869
BtrI CACGTC 1 cut(s) 61
BtsCI GGATG 2 cut(s) 121, 423
BtsIMutI CAGTG 4 cut(s) 87, 408, 654, 668
BtuMI TCGCGA 1 cut(s) 377
ClaI ATCGAT 1 cut(s) 869
CseI GACGC 1 cut(s) 865
Csp6I GTAC 2 cut(s) 622, 697
CviAII CATG 5 cut(s) 112, 145, 731, 823, 908
CviQI GTAC 2 cut(s) 622, 697
DdeI CTNAG 8 cut(s) 42, 90, 135, 267, 830, 885, 936, 951
DpnI GATC 6 cut(s) 12, 399, 516, 594, 743, 872
DpnII GATC 6 cut(s) 10, 397, 514, 592, 741, 870
Ecl136II GAGCTC 1 cut(s) 46
Eco147I AGGCCT 1 cut(s) 934
Eco24I GRGCYC 1 cut(s) 48
Eco53kI GAGCTC 1 cut(s) 46
Eco57I CTGAAG 1 cut(s) 845
Eco81I CCTNAGG 1 cut(s) 885
Eco88I CYCGRG 1 cut(s) 510
EcoICRI GAGCTC 1 cut(s) 46
EcoNI CCTNNNNNAGG 1 cut(s) 884
EcoRII CCWGG 1 cut(s) 283
EcoT38I GRGCYC 1 cut(s) 48
Esp3I CGTCTC 2 cut(s) 749, 848
FaeI CATG 5 cut(s) 115, 148, 734, 826, 911
FatI CATG 5 cut(s) 111, 144, 730, 822, 907
FblI GTMKAC 1 cut(s) 912
Fnu4HI GCNGC 4 cut(s) 365, 555, 558, 781
FokI GGATG 2 cut(s) 128, 430
FriOI GRGCYC 1 cut(s) 48
Fsp4HI GCNGC 4 cut(s) 365, 555, 558, 781
GluI GCNGC 4 cut(s) 365, 555, 558, 781
HaeIII GGCC 3 cut(s) 162, 283, 934
HapII CCGG 3 cut(s) 37, 56, 816
HgaI GACGC 1 cut(s) 865
Hin1II CATG 5 cut(s) 115, 148, 734, 826, 911
HincII GTYRAC 1 cut(s) 913
HindII GTYRAC 1 cut(s) 913
HinfI GANTC 9 cut(s) 23, 120, 149, 243, 257, 356, 382, 480, 866
HpaII CCGG 3 cut(s) 37, 56, 816
HphI GGTGA 1 cut(s) 772
Hpy166II GTNNAC 3 cut(s) 178, 624, 913
Hpy188I TCNGA 4 cut(s) 31, 268, 720, 851
Hpy188III TCNNGA 6 cut(s) 376, 484, 512, 863, 884, 966
Hpy8I GTNNAC 3 cut(s) 178, 624, 913
HpyAV CCTTC 2 cut(s) 137, 890
HpyCH4III ACNGT 5 cut(s) 76, 82, 445, 649, 760
HpyCH4IV ACGT 1 cut(s) 60
HpyCH4V TGCA 5 cut(s) 206, 317, 440, 554, 783
HpyF10VI GCNNNNNNNGC 3 cut(s) 52, 566, 956
HpyF3I CTNAG 8 cut(s) 42, 90, 135, 267, 830, 885, 936, 951
HpySE526I ACGT 1 cut(s) 60
Hsp92II CATG 5 cut(s) 115, 148, 734, 826, 911
Kzo9I GATC 6 cut(s) 10, 397, 514, 592, 741, 870
LmnI GCTCC 1 cut(s) 602
Lsp1109I GCAGC 2 cut(s) 566, 767
LweI GCATC 2 cut(s) 104, 326
MaeII ACGT 1 cut(s) 60
MaeIII GTNAC 3 cut(s) 61, 76, 666
MalI GATC 6 cut(s) 12, 399, 516, 594, 743, 872
MboI GATC 6 cut(s) 10, 397, 514, 592, 741, 870
MboII GAAGA 4 cut(s) 5, 18, 391, 691
MflI RGATCY 2 cut(s) 10, 397
MhlI GDGCHC 2 cut(s) 48, 942
MluCI AATT 5 cut(s) 201, 492, 543, 634, 707
MlyI GAGTC 2 cut(s) 251, 376
MmeI TCCRAC 1 cut(s) 580
MnlI CCTC 7 cut(s) 85, 96, 173, 183, 213, 790, 880
MroXI GAANNNNTTC 2 cut(s) 495, 879
MseI TTAA 2 cut(s) 185, 293
MslI CAYNNNNRTG 1 cut(s) 945
MspA1I CMGCKG 1 cut(s) 134
MspI CCGG 3 cut(s) 37, 56, 816
MspR9I CCNGG 2 cut(s) 285, 816
MvaI CCWGG 1 cut(s) 285
MvnI CGCG 1 cut(s) 377
MwoI GCNNNNNNNGC 3 cut(s) 52, 566, 956
NciI CCSGG 1 cut(s) 816
NdeII GATC 6 cut(s) 10, 397, 514, 592, 741, 870
NlaIII CATG 5 cut(s) 115, 148, 734, 826, 911
NlaIV GGNNCC 2 cut(s) 399, 728
NmeAIII GCCGAG 1 cut(s) 144
NmuCI GTSAC 3 cut(s) 61, 76, 666
NruI TCGCGA 1 cut(s) 377
NspI RCATGY 1 cut(s) 826
NspV TTCGAA 1 cut(s) 16
PaeR7I CTCGAG 1 cut(s) 510
PceI AGGCCT 1 cut(s) 934
PciI ACATGT 1 cut(s) 822
PcsI WCGNNNNNNNCGW 1 cut(s) 909
PdmI GAANNNNTTC 2 cut(s) 495, 879
PfeI GAWTC 7 cut(s) 23, 120, 149, 243, 356, 480, 866
PflFI GACNNNGTC 1 cut(s) 806
PkrI GCNGC 4 cut(s) 366, 556, 559, 782
Ple19I CGATCG 1 cut(s) 873
PleI GAGTC 2 cut(s) 251, 376
PpsI GAGTC 2 cut(s) 251, 376
PscI ACATGT 1 cut(s) 822
Psp124BI GAGCTC 1 cut(s) 48
Psp6I CCWGG 1 cut(s) 283
PspGI CCWGG 1 cut(s) 283
PspN4I GGNNCC 2 cut(s) 399, 728
PsuI RGATCY 2 cut(s) 10, 397
PsyI GACNNNGTC 1 cut(s) 806
PvuI CGATCG 1 cut(s) 873
PvuII CAGCTG 1 cut(s) 134
RruI TCGCGA 1 cut(s) 377
RsaI GTAC 2 cut(s) 623, 698
RsaNI GTAC 2 cut(s) 622, 697
RseI CAYNNNNRTG 1 cut(s) 945
SacI GAGCTC 1 cut(s) 48
SalI GTCGAC 1 cut(s) 911
SaqAI TTAA 2 cut(s) 185, 293
SatI GCNGC 4 cut(s) 365, 555, 558, 781
Sau3AI GATC 6 cut(s) 10, 397, 514, 592, 741, 870
ScaI AGTACT 1 cut(s) 698
SchI GAGTC 2 cut(s) 251, 376
ScrFI CCNGG 2 cut(s) 285, 816
SduI GDGCHC 2 cut(s) 48, 942
SfaNI GCATC 2 cut(s) 104, 326
Sfr274I CTCGAG 1 cut(s) 510
SfuI TTCGAA 1 cut(s) 16
SlaI CTCGAG 1 cut(s) 510
SmiMI CAYNNNNRTG 1 cut(s) 945
SmlI CTYRAG 1 cut(s) 510
SmoI CTYRAG 1 cut(s) 510
Sse9I AATT 5 cut(s) 201, 492, 543, 634, 707
SseBI AGGCCT 1 cut(s) 934
SsiI CCGC 3 cut(s) 8, 364, 558
SspI AATATT 2 cut(s) 212, 348
SstI GAGCTC 1 cut(s) 48
StuI AGGCCT 1 cut(s) 934
StyD4I CCNGG 2 cut(s) 283, 814
TaaI ACNGT 5 cut(s) 76, 82, 445, 649, 760
TaiI ACGT 1 cut(s) 63
TaqI TCGA 6 cut(s) 16, 499, 511, 869, 873, 912
TasI AATT 5 cut(s) 201, 492, 543, 634, 707
TatI WGTACW 2 cut(s) 621, 696
TauI GCSGC 2 cut(s) 367, 560
TfiI GAWTC 7 cut(s) 23, 120, 149, 243, 356, 480, 866
Tru1I TTAA 2 cut(s) 185, 293
Tru9I TTAA 2 cut(s) 185, 293
TscAI CASTG 4 cut(s) 87, 408, 654, 675
TseFI GTSAC 3 cut(s) 61, 76, 666
TseI GCWGC 2 cut(s) 554, 780
Tsp45I GTSAC 3 cut(s) 61, 76, 666
TspDTI ATGAA 1 cut(s) 896
TspRI CASTG 4 cut(s) 87, 408, 654, 675
Tth111I GACNNNGTC 1 cut(s) 806
XagI CCTNNNNNAGG 1 cut(s) 884
XceI RCATGY 1 cut(s) 826
XhoI CTCGAG 1 cut(s) 510
XmiI GTMKAC 1 cut(s) 912
XmnI GAANNNNTTC 2 cut(s) 495, 879
ZrmI AGTACT 1 cut(s) 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.