FvH4_3g14571

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001629
Physical Location & Seq
Reverse (-)
8990869 .. 8993231
2363 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g14571.t2

Sequence Viewer

Length: 1206 bp
ATGGTGACAGAACTTCGAACAGATTCTTTGGATTACCGCACAGAGCTGTTGTCGCCGGATCTGACAAGAGAGAGTGTGACACTGCCAAGTGAACCGGCGTGGCGGCTCAACATGGATGGCTTCAAGCTTCCTGCCCAGAGAAACAACATGGATCCTTTTTTTGGCTTTGGCTCTTTTGTGAAGTCATTAAGGACTCGGAGAAAGGTTGCACAGTATTACAGAAGGCAGAACAAACTTCTCAAAGGTTTCAATGAATTAGACTCATTTTCTGAAATGGGTTTTTGGCCTGGAAGTTTAACAAAGGATGAGATGACACAGCTTGCAAGTAGTGAAAGGATAGCAATATATGCATCGAATGCAGCTAATTTGGTGCTTTTCCTAGCAAAAGTGTATGCTTCTATTCAGAGCAGATCATTGGCAGTAATAGCATCAACTTTGGACTCCCTCTTAGACCTCTTGTCAGGGTTCATCCTTTGGTTTACAGCTTCTGCAATGAGGAAACCAAATCAGTATCGCTACCCGATTGGCAAGACTCGGATGCAACCTGTGGGAATTGTTGTGTTTGCATCAGTAATGGCAACTCTTGGATTGCAAATATTGTTCGAATCAGGCCGACAACTTGTCATGAAGACTCAACCTGATAAGGACCCAGAGAAAGAGAAATGGATGATAGGGATTATGGTCTCTGTCACATTCATCAAGTTTGTTCTAATGATCTATTGTCGAAGATTCAAAAACGAAATTGTTCGAGCATATGCTCAAGATCATCTATTTGATGTCATCACTAATGCAATTGGTCTTGCATCAGCAGTGTTAGCTGTCCGATTTTACTGGTGGATAGATCCGGTTGGAGCTATCATCATTGCTTTGTATACAATGGGGAATTGGTCAAAAACAGTGATGGACAATGTATGGTCACTAATTGGGAAGACAGCACCGGCAGAGTACTTGGCAAAGTTAACATATCTAATTTGGAACCATGACAAGGAGATCAAGCACATTGAGACAGTGAGAGCATACACATTTGGTGTTAACTATTTTGTGGAGGTTCATATAGTCCTACCTGGGGACATGTCTCTCAGTGATGCGCATAATATCGGAGAGGCACTCCAAGAAAAGCTTGAGAATCTTCCTGAGGTTGAACGAGCTTTTGTTCATGTGGATGTTGACATTACTCACAAACCAGAACACAAGCCAAAGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux ZT_dimer

Protein Analysis

402

Amino Acids

45.76

Weight (kDa)

8.85

Isoelectric Point (pI)

39.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_efflux PF01545 45 - 235 5.7e-34 Cation efflux transmembrane domain
ZT_dimer PF16916 247 - 316 1.8e-10 Cation efflux protein, cytoplasmic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1089
AccI GTMKAC 1 cut(s) 872
AciI CCGC 2 cut(s) 37, 103
AclWI GGATC 4 cut(s) 66, 146, 159, 836
AfaI GTAC 1 cut(s) 947
AfiI CCNNNNNNNGG 3 cut(s) 161, 985, 1066
AflIII ACRYGT 1 cut(s) 1071
AgsI TTSAA 4 cut(s) 124, 250, 733, 1142
AhdI GACNNNNNGTC 2 cut(s) 457, 620
AjnI CCWGG 2 cut(s) 286, 1063
AluBI AGCT 9 cut(s) 46, 127, 319, 362, 485, 818, 854, 1120, 1148
AluI AGCT 9 cut(s) 46, 127, 319, 362, 485, 818, 854, 1120, 1148
Alw26I GTCTC 3 cut(s) 688, 998, 1080
AlwI GGATC 4 cut(s) 66, 146, 159, 836
AlwNI CAGNNNCTG 1 cut(s) 488
AoxI GGCC 2 cut(s) 284, 610
ApeKI GCWGC 1 cut(s) 359
Asp700I GAANNNNTTC 2 cut(s) 22, 744
AspLEI GCGC 1 cut(s) 1090
AspS9I GGNCC 1 cut(s) 646
AsuHPI GGTGA 1 cut(s) 16
AsuII TTCGAA 2 cut(s) 16, 603
AvaII GGWCC 1 cut(s) 646
AxyI CCTNAGG 1 cut(s) 1134
BamHI GGATCC 1 cut(s) 151
BbsI GAAGAC 2 cut(s) 635, 935
BbvI GCAGC 1 cut(s) 371
BccI CCATC 2 cut(s) 110, 895
BciT130I CCWGG 2 cut(s) 288, 1065
BcoDI GTCTC 3 cut(s) 688, 998, 1080
BfaI CTAG 1 cut(s) 380
BisI GCNGC 2 cut(s) 104, 360
BlsI GCNGC 2 cut(s) 105, 361
BmcAI AGTACT 1 cut(s) 947
Bme1390I CCNGG 2 cut(s) 288, 1065
Bme18I GGWCC 1 cut(s) 646
BmeRI GACNNNNNGTC 2 cut(s) 457, 620
BmgT120I GGNCC 1 cut(s) 646
BmiI GGNNCC 3 cut(s) 153, 648, 977
BmrFI CCNGG 2 cut(s) 288, 1065
BmsI GCATC 6 cut(s) 359, 437, 528, 575, 812, 1075
BpiI GAAGAC 2 cut(s) 635, 935
BplI GAGNNNNNCTC 2 cut(s) 1092, 1124
Bpu14I TTCGAA 2 cut(s) 16, 603
BpuEI CTTGAG 2 cut(s) 744, 1142
BsaI GGTCTC 1 cut(s) 688
BsaJI CCNNGG 1 cut(s) 1064
BsaWI WCCGGW 1 cut(s) 844
Bsc4I CCNNNNNNNGG 3 cut(s) 161, 985, 1066
Bse118I RCCGGY 2 cut(s) 94, 937
Bse1I ACTGG 1 cut(s) 836
Bse21I CCTNAGG 1 cut(s) 1134
Bse3DI GCAATG 2 cut(s) 498, 861
BseBI CCWGG 2 cut(s) 288, 1065
BseDI CCNNGG 1 cut(s) 1064
BseGI GGATG 6 cut(s) 121, 310, 468, 543, 672, 1168
BseLI CCNNNNNNNGG 3 cut(s) 161, 985, 1066
BseMI GCAATG 2 cut(s) 498, 861
BseMII CTCAG 2 cut(s) 1093, 1125
BseNI ACTGG 1 cut(s) 836
BseXI GCAGC 1 cut(s) 371
BshFI GGCC 2 cut(s) 286, 612
BsiSI CCGG 4 cut(s) 56, 95, 845, 938
BslFI GGGAC 1 cut(s) 1082
BslI CCNNNNNNNGG 3 cut(s) 161, 985, 1066
BsmAI GTCTC 3 cut(s) 688, 998, 1080
BsmFI GGGAC 1 cut(s) 1082
BsmI GAATGC 1 cut(s) 361
BsnI GGCC 2 cut(s) 286, 612
Bso31I GGTCTC 1 cut(s) 688
Bsp119I TTCGAA 2 cut(s) 16, 603
Bsp143I GATC 7 cut(s) 58, 151, 410, 714, 763, 841, 990
BspACI CCGC 2 cut(s) 37, 103
BspANI GGCC 2 cut(s) 286, 612
BspCNI CTCAG 2 cut(s) 1092, 1126
BspHI TCATGA 1 cut(s) 624
BspLI GGNNCC 3 cut(s) 153, 648, 977
BspPI GGATC 4 cut(s) 66, 146, 159, 836
BspT104I TTCGAA 2 cut(s) 16, 603
BspTNI GGTCTC 1 cut(s) 688
BsrDI GCAATG 2 cut(s) 498, 861
BsrFI RCCGGY 2 cut(s) 94, 937
BsrI ACTGG 1 cut(s) 836
BssAI RCCGGY 2 cut(s) 94, 937
BssECI CCNNGG 1 cut(s) 1064
BssMI GATC 7 cut(s) 58, 151, 410, 714, 763, 841, 990
BssNAI GTATAC 1 cut(s) 873
Bst1107I GTATAC 1 cut(s) 873
Bst2UI CCWGG 2 cut(s) 288, 1065
Bst4CI ACNGT 3 cut(s) 213, 898, 1009
BstAPI GCANNNNNTGC 2 cut(s) 347, 356
BstBI TTCGAA 2 cut(s) 16, 603
BstC8I GCNNGC 1 cut(s) 321
BstDEI CTNAG 3 cut(s) 448, 1079, 1134
BstF5I GGATG 6 cut(s) 121, 310, 468, 543, 672, 1168
BstHHI GCGC 1 cut(s) 1090
BstKTI GATC 7 cut(s) 61, 154, 413, 717, 766, 844, 993
BstMAI GTCTC 3 cut(s) 688, 998, 1080
BstMBI GATC 7 cut(s) 58, 151, 410, 714, 763, 841, 990
BstMWI GCNNNNNNNGC 5 cut(s) 52, 347, 356, 425, 815
BstNI CCWGG 2 cut(s) 288, 1065
BstNSI RCATGY 1 cut(s) 1075
BstSCI CCNGG 2 cut(s) 286, 1063
BstV1I GCAGC 1 cut(s) 371
BstV2I GAAGAC 2 cut(s) 635, 935
BstX2I RGATCY 3 cut(s) 58, 151, 841
BstYI RGATCY 3 cut(s) 58, 151, 841
BstZ17I GTATAC 1 cut(s) 873
Bsu36I CCTNAGG 1 cut(s) 1134
BsuRI GGCC 2 cut(s) 286, 612
BtsCI GGATG 6 cut(s) 121, 310, 468, 543, 672, 1168
BtsI GCAGTG 2 cut(s) 80, 816
BtsIMutI CAGTG 5 cut(s) 80, 816, 903, 1014, 1087
Cac8I GCNNGC 1 cut(s) 321
CaiI CAGNNNCTG 1 cut(s) 488
CciI TCATGA 1 cut(s) 624
CfoI GCGC 1 cut(s) 1090
Cfr10I RCCGGY 2 cut(s) 94, 937
Cfr13I GGNCC 1 cut(s) 646
Csp6I GTAC 1 cut(s) 946
CviAII CATG 6 cut(s) 112, 148, 625, 980, 1072, 1157
CviQI GTAC 1 cut(s) 946
DdeI CTNAG 3 cut(s) 448, 1079, 1134
DpnI GATC 7 cut(s) 60, 153, 412, 716, 765, 843, 992
DpnII GATC 7 cut(s) 58, 151, 410, 714, 763, 841, 990
DriI GACNNNNNGTC 2 cut(s) 457, 620
Eam1105I GACNNNNNGTC 2 cut(s) 457, 620
Eco31I GGTCTC 1 cut(s) 688
Eco47I GGWCC 1 cut(s) 646
Eco81I CCTNAGG 1 cut(s) 1134
EcoO109I RGGNCCY 1 cut(s) 646
EcoRII CCWGG 2 cut(s) 286, 1063
EcoT22I ATGCAT 1 cut(s) 352
FaeI CATG 6 cut(s) 115, 151, 628, 983, 1075, 1160
FalI AAGNNNNNCTT 2 cut(s) 1104, 1136
FaqI GGGAC 1 cut(s) 1082
FatI CATG 6 cut(s) 111, 147, 624, 979, 1071, 1156
FauNDI CATATG 1 cut(s) 754
FblI GTMKAC 1 cut(s) 872
Fnu4HI GCNGC 2 cut(s) 104, 360
FokI GGATG 6 cut(s) 128, 317, 455, 550, 679, 1175
Fsp4HI GCNGC 2 cut(s) 104, 360
FspAI RTGCGCAY 1 cut(s) 1089
FspBI CTAG 1 cut(s) 380
FspI TGCGCA 1 cut(s) 1089
GlaI GCGC 1 cut(s) 1089
GluI GCNGC 2 cut(s) 104, 360
HaeIII GGCC 2 cut(s) 286, 612
HapII CCGG 4 cut(s) 56, 95, 845, 938
HhaI GCGC 1 cut(s) 1090
Hin1II CATG 6 cut(s) 115, 151, 628, 983, 1075, 1160
Hin6I GCGC 1 cut(s) 1088
HinP1I GCGC 1 cut(s) 1088
HincII GTYRAC 3 cut(s) 960, 1033, 1168
HindII GTYRAC 3 cut(s) 960, 1033, 1168
HindIII AAGCTT 2 cut(s) 125, 1118
HinfI GANTC 9 cut(s) 23, 193, 260, 440, 532, 605, 631, 729, 1126
HpaI GTTAAC 2 cut(s) 960, 1033
HpaII CCGG 4 cut(s) 56, 95, 845, 938
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 6 cut(s) 92, 480, 873, 960, 1033, 1168
Hpy188I TCNGA 7 cut(s) 63, 198, 271, 405, 537, 824, 1100
Hpy188III TCNNGA 3 cut(s) 625, 761, 1133
Hpy8I GTNNAC 6 cut(s) 92, 480, 873, 960, 1033, 1168
HpyAV CCTTC 1 cut(s) 216
HpyCH4III ACNGT 3 cut(s) 213, 898, 1009
HpyF10VI GCNNNNNNNGC 5 cut(s) 52, 347, 356, 425, 815
HpyF3I CTNAG 3 cut(s) 448, 1079, 1134
Hsp92II CATG 6 cut(s) 115, 151, 628, 983, 1075, 1160
HspAI GCGC 1 cut(s) 1088
KspAI GTTAAC 2 cut(s) 960, 1033
Kzo9I GATC 7 cut(s) 58, 151, 410, 714, 763, 841, 990
LmnI GCTCC 1 cut(s) 851
Lsp1109I GCAGC 1 cut(s) 371
LweI GCATC 6 cut(s) 359, 437, 528, 575, 812, 1075
MaeI CTAG 1 cut(s) 380
MaeIII GTNAC 4 cut(s) 4, 76, 688, 915
MalI GATC 7 cut(s) 60, 153, 412, 716, 765, 843, 992
MboI GATC 7 cut(s) 58, 151, 410, 714, 763, 841, 990
MboII GAAGA 4 cut(s) 640, 738, 940, 1121
MfeI CAATTG 1 cut(s) 792
MflI RGATCY 3 cut(s) 58, 151, 841
MluCI AATT 8 cut(s) 254, 364, 552, 741, 792, 883, 921, 969
MlyI GAGTC 5 cut(s) 187, 254, 434, 526, 625
MmeI TCCRAC 1 cut(s) 829
MnlI CCTC 6 cut(s) 455, 464, 489, 1039, 1096, 1129
Mph1103I ATGCAT 1 cut(s) 352
MroXI GAANNNNTTC 2 cut(s) 22, 744
MseI TTAA 4 cut(s) 188, 296, 959, 1032
MslI CAYNNNNRTG 1 cut(s) 1161
MspI CCGG 4 cut(s) 56, 95, 845, 938
MspR9I CCNGG 2 cut(s) 288, 1065
MunI CAATTG 1 cut(s) 792
Mva1269I GAATGC 1 cut(s) 361
MvaI CCWGG 2 cut(s) 288, 1065
MwoI GCNNNNNNNGC 5 cut(s) 52, 347, 356, 425, 815
NdeI CATATG 1 cut(s) 754
NdeII GATC 7 cut(s) 58, 151, 410, 714, 763, 841, 990
NlaIII CATG 6 cut(s) 115, 151, 628, 983, 1075, 1160
NlaIV GGNNCC 3 cut(s) 153, 648, 977
NmuCI GTSAC 4 cut(s) 4, 76, 688, 915
NsbI TGCGCA 1 cut(s) 1089
NsiI ATGCAT 1 cut(s) 352
NspI RCATGY 1 cut(s) 1075
NspV TTCGAA 2 cut(s) 16, 603
PagI TCATGA 1 cut(s) 624
PciI ACATGT 1 cut(s) 1071
PctI GAATGC 1 cut(s) 361
PdmI GAANNNNTTC 2 cut(s) 22, 744
PfeI GAWTC 4 cut(s) 23, 605, 729, 1126
PkrI GCNGC 2 cut(s) 105, 361
PleI GAGTC 5 cut(s) 187, 254, 434, 526, 625
PpsI GAGTC 5 cut(s) 187, 254, 434, 526, 625
PpuMI RGGWCCY 1 cut(s) 646
PscI ACATGT 1 cut(s) 1071
Psp5II RGGWCCY 1 cut(s) 646
Psp6I CCWGG 2 cut(s) 286, 1063
PspGI CCWGG 2 cut(s) 286, 1063
PspN4I GGNNCC 3 cut(s) 153, 648, 977
PspPI GGNCC 1 cut(s) 646
PspPPI RGGWCCY 1 cut(s) 646
PstNI CAGNNNCTG 1 cut(s) 488
PsuI RGATCY 3 cut(s) 58, 151, 841
RsaI GTAC 1 cut(s) 947
RsaNI GTAC 1 cut(s) 946
RseI CAYNNNNRTG 1 cut(s) 1161
SaqAI TTAA 4 cut(s) 188, 296, 959, 1032
SatI GCNGC 2 cut(s) 104, 360
Sau3AI GATC 7 cut(s) 58, 151, 410, 714, 763, 841, 990
Sau96I GGNCC 1 cut(s) 646
ScaI AGTACT 1 cut(s) 947
SchI GAGTC 5 cut(s) 187, 254, 434, 526, 625
ScrFI CCNGG 2 cut(s) 288, 1065
SfaNI GCATC 6 cut(s) 359, 437, 528, 575, 812, 1075
SfuI TTCGAA 2 cut(s) 16, 603
SinI GGWCC 1 cut(s) 646
SmiMI CAYNNNNRTG 1 cut(s) 1161
SmlI CTYRAG 2 cut(s) 759, 1121
SmoI CTYRAG 2 cut(s) 759, 1121
Sse9I AATT 8 cut(s) 254, 364, 552, 741, 792, 883, 921, 969
SsiI CCGC 2 cut(s) 37, 103
SspI AATATT 1 cut(s) 597
SspMI CTAG 1 cut(s) 380
StyD4I CCNGG 2 cut(s) 286, 1063
TaaI ACNGT 3 cut(s) 213, 898, 1009
TaqI TCGA 5 cut(s) 16, 353, 603, 724, 748
TasI AATT 8 cut(s) 254, 364, 552, 741, 792, 883, 921, 969
TatI WGTACW 1 cut(s) 945
TauI GCSGC 1 cut(s) 106
TfiI GAWTC 4 cut(s) 23, 605, 729, 1126
Tru1I TTAA 4 cut(s) 188, 296, 959, 1032
Tru9I TTAA 4 cut(s) 188, 296, 959, 1032
TscAI CASTG 5 cut(s) 87, 816, 903, 1014, 1087
TseFI GTSAC 4 cut(s) 4, 76, 688, 915
TseI GCWGC 1 cut(s) 359
Tsp45I GTSAC 4 cut(s) 4, 76, 688, 915
TspDTI ATGAA 6 cut(s) 267, 457, 641, 685, 1040, 1145
TspRI CASTG 5 cut(s) 87, 816, 903, 1014, 1087
VpaK11BI GGWCC 1 cut(s) 646
XceI RCATGY 1 cut(s) 1075
XmiI GTMKAC 1 cut(s) 872
XmnI GAANNNNTTC 2 cut(s) 22, 744
XspI CTAG 1 cut(s) 380
ZrmI AGTACT 1 cut(s) 947
Zsp2I ATGCAT 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.