Prupe.4G131200_v2.0.a1

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0001629
Physical Location & Seq
Reverse (-)
7244756 .. 7247211
2456 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G131200.2

Sequence Viewer

Length: 1206 bp
ATGGTGACAGATCTTCGAACAGATTCTTCGGATTACCGGACTGAGCTTCTGTCGCCGGATGTGACAGGAGAGGCTGTGACAATGCCAACTGAGGCAGCATGGAGGCTCAATATGGATGGCTTCAAGCTTCCAGACAGAAACAAGGATTCTTATTTTGGCTTTGGCTCTTTTGTTGAGTCATTAAGGAGGCAAAGAAAAATTGCAGAATATTACAAGAGGCAGAATAAACTTCTCAAAGGATTCAATGAGTTGGACTTATTTTCAGAAACGGGGTTTTGGCCTGGAAGTTTAACAGAGGATGAGGTGAACCAGCTTGCAAGAAATGAAAGGATAGCAATATATGCATCCAATGTGGCTAATTTGGTGCTTTTCCTGGCAAAAGTGTATGCTTCATTTGAGAGCAGATCATTAGCAGTAATAGCTTCAACATTGGATTCTCTGTTAGACCTCTTGTCAGGGTTCATTCTTTGGTTTACATCCAATGCAATGAGAAAACCAAATCAATACCGGTACCCGATTGGCAAGAATCGGATGCAGCCTGTGGGAATTGTTGTTTTTGCATCAGTAATGGCAACTCTTGGATTACAAATCTTGTTCGAATCGGGACGGCAACTTCTCACAAAGGCTCAACCTGATAGGGACCCAGAGAAAGAAAAATGGATGATAGGGATCATGGTGTCTGCCACAGTGGTGAAGTTTGTGCTGATGGCATATTGTCGCAGATTCAAAAACGAAATTGTTCGAGCATATGCTCAAGATCATCTATTTGATGTCATCACTAATGGGATTGGTCTTGCATCAGCAGTTTTAGCAATCAGGTTTTACTGGTGGATTGACCCTGTTGGAGCTATCATTATTGCTTTGTATACAATGGGAAATTGGGCAAAAACAGTGATGGAAAATGTGTGGTCACTGATTGGGAAGACAGCACCAGCAGAGTACCTGGCAAAGTTGACATATCTGATTTGGAACCATGACAAGGAGATCCAGCACATTGAGACAGTGAGAGCCTATACTTTTGGTTGCAACTATTTTGTGGAAGTTGACATAGTCTTGCCTGGGGACATGTCTCTTAGTCATGCACATAACATTGGAGAAACACTTCAAGAGAAGCTTGAAGTCCTTCCTGAGGTTGAAAGAGCTTTTGTTCATGTGGACTTTGATATTACTCACAGGCCAGAGCACAAGCCAAAGTTGGCTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux ZT_dimer

Protein Analysis

402

Amino Acids

45.76

Weight (kDa)

6.28

Isoelectric Point (pI)

35.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 510
AccB1I GGYRCC 1 cut(s) 510
AccI GTMKAC 1 cut(s) 866
AclWI GGATC 2 cut(s) 677, 979
AfaI GTAC 2 cut(s) 512, 941
AfiI CCNNNNNNNGG 2 cut(s) 979, 1129
AflIII ACRYGT 1 cut(s) 1065
AgeI ACCGGT 1 cut(s) 507
AgsI TTSAA 7 cut(s) 124, 244, 426, 727, 1106, 1118, 1136
AhdI GACNNNNNGTC 1 cut(s) 451
AjnI CCWGG 4 cut(s) 280, 372, 942, 1057
AjuI GAANNNNNNNTTGG 1 cut(s) 1183
AleI CACNNNNGTG 1 cut(s) 689
AluBI AGCT 7 cut(s) 46, 127, 313, 422, 848, 1114, 1142
AluI AGCT 7 cut(s) 46, 127, 313, 422, 848, 1114, 1142
Alw21I GWGCWC 1 cut(s) 1185
Alw26I GTCTC 2 cut(s) 992, 1074
AlwI GGATC 2 cut(s) 677, 979
AoxI GGCC 2 cut(s) 278, 1175
ApeKI GCWGC 2 cut(s) 95, 535
AsiGI ACCGGT 1 cut(s) 507
Asp700I GAANNNNTTC 4 cut(s) 22, 738, 1101, 1122
Asp718I GGTACC 1 cut(s) 510
AspS9I GGNCC 1 cut(s) 640
AsuHPI GGTGA 3 cut(s) 16, 316, 703
AsuII TTCGAA 2 cut(s) 16, 597
AvaII GGWCC 1 cut(s) 640
AxyI CCTNAGG 1 cut(s) 1128
BanI GGYRCC 1 cut(s) 510
BbsI GAAGAC 1 cut(s) 929
Bbv12I GWGCWC 1 cut(s) 1185
BbvI GCAGC 2 cut(s) 107, 547
BccI CCATC 3 cut(s) 110, 700, 889
BceAI ACGGC 1 cut(s) 623
BciT130I CCWGG 4 cut(s) 282, 374, 944, 1059
BcoDI GTCTC 2 cut(s) 992, 1074
BglII AGATCT 1 cut(s) 10
BisI GCNGC 2 cut(s) 96, 536
BlsI GCNGC 2 cut(s) 97, 537
Bme1390I CCNGG 4 cut(s) 282, 374, 944, 1059
Bme18I GGWCC 1 cut(s) 640
BmeRI GACNNNNNGTC 1 cut(s) 451
BmgT120I GGNCC 1 cut(s) 640
BmiI GGNNCC 4 cut(s) 512, 641, 642, 971
BmrFI CCNGG 4 cut(s) 282, 374, 944, 1059
BmsI GCATC 4 cut(s) 353, 522, 569, 806
BpiI GAAGAC 1 cut(s) 929
Bpu14I TTCGAA 2 cut(s) 16, 597
BpuEI CTTGAG 1 cut(s) 738
BsaBI GATNNNNATC 1 cut(s) 668
BsaJI CCNNGG 1 cut(s) 1058
BsaWI WCCGGW 2 cut(s) 36, 507
BsaXI ACNNNNNCTCC 2 cut(s) 60, 90
Bsc4I CCNNNNNNNGG 2 cut(s) 979, 1129
Bse118I RCCGGY 1 cut(s) 507
Bse1I ACTGG 1 cut(s) 830
Bse21I CCTNAGG 1 cut(s) 1128
Bse3DI GCAATG 1 cut(s) 492
Bse8I GATNNNNATC 1 cut(s) 668
BseBI CCWGG 4 cut(s) 282, 374, 944, 1059
BseDI CCNNGG 1 cut(s) 1058
BseGI GGATG 7 cut(s) 64, 121, 304, 344, 476, 537, 666
BseJI GATNNNNATC 1 cut(s) 668
BseLI CCNNNNNNNGG 2 cut(s) 979, 1129
BseMI GCAATG 1 cut(s) 492
BseMII CTCAG 3 cut(s) 33, 81, 1119
BseNI ACTGG 1 cut(s) 830
BseXI GCAGC 2 cut(s) 107, 547
BshFI GGCC 2 cut(s) 280, 1177
BshNI GGYRCC 1 cut(s) 510
BshTI ACCGGT 1 cut(s) 507
BsiHKAI GWGCWC 1 cut(s) 1185
BsiSI CCGG 3 cut(s) 37, 56, 508
BslFI GGGAC 3 cut(s) 618, 653, 1076
BslI CCNNNNNNNGG 2 cut(s) 979, 1129
BsmAI GTCTC 2 cut(s) 992, 1074
BsmFI GGGAC 3 cut(s) 618, 653, 1076
BsnI GGCC 2 cut(s) 280, 1177
Bsp119I TTCGAA 2 cut(s) 16, 597
Bsp1286I GDGCHC 1 cut(s) 1185
Bsp143I GATC 5 cut(s) 10, 404, 669, 757, 984
BspANI GGCC 2 cut(s) 280, 1177
BspCNI CTCAG 3 cut(s) 34, 82, 1120
BspHI TCATGA 1 cut(s) 1202
BspLI GGNNCC 4 cut(s) 512, 641, 642, 971
BspPI GGATC 2 cut(s) 677, 979
BspT104I TTCGAA 2 cut(s) 16, 597
BspT107I GGYRCC 1 cut(s) 510
BsrDI GCAATG 1 cut(s) 492
BsrFI RCCGGY 1 cut(s) 507
BsrI ACTGG 1 cut(s) 830
BssAI RCCGGY 1 cut(s) 507
BssECI CCNNGG 1 cut(s) 1058
BssMI GATC 5 cut(s) 10, 404, 669, 757, 984
BssNAI GTATAC 1 cut(s) 867
Bst1107I GTATAC 1 cut(s) 867
Bst2UI CCWGG 4 cut(s) 282, 374, 944, 1059
Bst4CI ACNGT 3 cut(s) 688, 892, 1003
BstAPI GCANNNNNTGC 1 cut(s) 341
BstBI TTCGAA 2 cut(s) 16, 597
BstC8I GCNNGC 1 cut(s) 315
BstDEI CTNAG 4 cut(s) 42, 90, 1073, 1128
BstENI CCTNNNNNAGG 1 cut(s) 1127
BstF5I GGATG 7 cut(s) 64, 121, 304, 344, 476, 537, 666
BstKTI GATC 5 cut(s) 13, 407, 672, 760, 987
BstMAI GTCTC 2 cut(s) 992, 1074
BstMBI GATC 5 cut(s) 10, 404, 669, 757, 984
BstMWI GCNNNNNNNGC 4 cut(s) 52, 341, 419, 809
BstNI CCWGG 4 cut(s) 282, 374, 944, 1059
BstNSI RCATGY 1 cut(s) 1069
BstSCI CCNGG 4 cut(s) 280, 372, 942, 1057
BstV1I GCAGC 2 cut(s) 107, 547
BstV2I GAAGAC 1 cut(s) 929
BstX2I RGATCY 2 cut(s) 10, 984
BstYI RGATCY 2 cut(s) 10, 984
BstZ17I GTATAC 1 cut(s) 867
Bsu36I CCTNAGG 1 cut(s) 1128
BsuRI GGCC 2 cut(s) 280, 1177
BtsCI GGATG 7 cut(s) 64, 121, 304, 344, 476, 537, 666
BtsIMutI CAGTG 4 cut(s) 693, 897, 911, 1008
Cac8I GCNNGC 1 cut(s) 315
CciI TCATGA 1 cut(s) 1202
Cfr10I RCCGGY 1 cut(s) 507
Cfr13I GGNCC 1 cut(s) 640
Csp6I GTAC 2 cut(s) 511, 940
CspAI ACCGGT 1 cut(s) 507
CviAII CATG 7 cut(s) 99, 673, 974, 1066, 1079, 1151, 1203
CviQI GTAC 2 cut(s) 511, 940
DdeI CTNAG 4 cut(s) 42, 90, 1073, 1128
DpnI GATC 5 cut(s) 12, 406, 671, 759, 986
DpnII GATC 5 cut(s) 10, 404, 669, 757, 984
DriI GACNNNNNGTC 1 cut(s) 451
Eam1105I GACNNNNNGTC 1 cut(s) 451
Eco47I GGWCC 1 cut(s) 640
Eco81I CCTNAGG 1 cut(s) 1128
EcoNI CCTNNNNNAGG 1 cut(s) 1127
EcoO109I RGGNCCY 1 cut(s) 640
EcoRII CCWGG 4 cut(s) 280, 372, 942, 1057
EcoT22I ATGCAT 1 cut(s) 346
FaeI CATG 7 cut(s) 102, 676, 977, 1069, 1082, 1154, 1206
FalI AAGNNNNNCTT 2 cut(s) 1098, 1130
FaqI GGGAC 3 cut(s) 618, 653, 1076
FatI CATG 7 cut(s) 98, 672, 973, 1065, 1078, 1150, 1202
FauNDI CATATG 1 cut(s) 748
FblI GTMKAC 1 cut(s) 866
Fnu4HI GCNGC 2 cut(s) 96, 536
FokI GGATG 7 cut(s) 71, 128, 311, 331, 463, 544, 673
Fsp4HI GCNGC 2 cut(s) 96, 536
GluI GCNGC 2 cut(s) 96, 536
HaeIII GGCC 2 cut(s) 280, 1177
HapII CCGG 3 cut(s) 37, 56, 508
Hin1II CATG 7 cut(s) 102, 676, 977, 1069, 1082, 1154, 1206
HincII GTYRAC 2 cut(s) 954, 1045
HindII GTYRAC 2 cut(s) 954, 1045
HindIII AAGCTT 2 cut(s) 125, 1112
HinfI GANTC 8 cut(s) 23, 146, 176, 240, 434, 526, 599, 723
HpaII CCGG 3 cut(s) 37, 56, 508
HphI GGTGA 3 cut(s) 16, 316, 703
Hpy166II GTNNAC 6 cut(s) 307, 474, 867, 954, 1045, 1156
Hpy188I TCNGA 4 cut(s) 31, 265, 531, 963
Hpy188III TCNNGA 6 cut(s) 131, 603, 755, 1106, 1127, 1203
Hpy8I GTNNAC 6 cut(s) 307, 474, 867, 954, 1045, 1156
HpyAV CCTTC 1 cut(s) 1133
HpyCH4III ACNGT 3 cut(s) 688, 892, 1003
HpyCH4V TGCA 9 cut(s) 203, 317, 344, 485, 535, 560, 797, 1026, 1082
HpyF10VI GCNNNNNNNGC 4 cut(s) 52, 341, 419, 809
HpyF3I CTNAG 4 cut(s) 42, 90, 1073, 1128
Hsp92II CATG 7 cut(s) 102, 676, 977, 1069, 1082, 1154, 1206
KflI GGGWCCC 1 cut(s) 640
KpnI GGTACC 1 cut(s) 514
Kzo9I GATC 5 cut(s) 10, 404, 669, 757, 984
LmnI GCTCC 1 cut(s) 845
Lsp1109I GCAGC 2 cut(s) 107, 547
LweI GCATC 4 cut(s) 353, 522, 569, 806
MaeIII GTNAC 4 cut(s) 4, 61, 76, 909
MalI GATC 5 cut(s) 12, 406, 671, 759, 986
MboI GATC 5 cut(s) 10, 404, 669, 757, 984
MboII GAAGA 3 cut(s) 5, 18, 934
MflI RGATCY 2 cut(s) 10, 984
MhlI GDGCHC 1 cut(s) 1185
MluCI AATT 5 cut(s) 198, 358, 546, 735, 877
MlyI GAGTC 1 cut(s) 185
MmeI TCCRAC 2 cut(s) 231, 823
MnlI CCTC 9 cut(s) 64, 85, 96, 180, 210, 289, 295, 458, 1123
Mph1103I ATGCAT 1 cut(s) 346
MroXI GAANNNNTTC 4 cut(s) 22, 738, 1101, 1122
MseI TTAA 2 cut(s) 182, 290
MslI CAYNNNNRTG 1 cut(s) 689
MspI CCGG 3 cut(s) 37, 56, 508
MspR9I CCNGG 4 cut(s) 282, 374, 944, 1059
MvaI CCWGG 4 cut(s) 282, 374, 944, 1059
MwoI GCNNNNNNNGC 4 cut(s) 52, 341, 419, 809
NdeI CATATG 1 cut(s) 748
NdeII GATC 5 cut(s) 10, 404, 669, 757, 984
NlaIII CATG 7 cut(s) 102, 676, 977, 1069, 1082, 1154, 1206
NlaIV GGNNCC 4 cut(s) 512, 641, 642, 971
NmuCI GTSAC 4 cut(s) 4, 61, 76, 909
NsiI ATGCAT 1 cut(s) 346
NspI RCATGY 1 cut(s) 1069
NspV TTCGAA 2 cut(s) 16, 597
OliI CACNNNNGTG 1 cut(s) 689
PagI TCATGA 1 cut(s) 1202
PciI ACATGT 1 cut(s) 1065
PdmI GAANNNNTTC 4 cut(s) 22, 738, 1101, 1122
PfeI GAWTC 7 cut(s) 23, 146, 240, 434, 526, 599, 723
PflFI GACNNNGTC 1 cut(s) 1049
PinAI ACCGGT 1 cut(s) 507
PkrI GCNGC 2 cut(s) 97, 537
PleI GAGTC 1 cut(s) 184
PpsI GAGTC 1 cut(s) 184
PpuMI RGGWCCY 1 cut(s) 640
PscI ACATGT 1 cut(s) 1065
Psp5II RGGWCCY 1 cut(s) 640
Psp6I CCWGG 4 cut(s) 280, 372, 942, 1057
PspGI CCWGG 4 cut(s) 280, 372, 942, 1057
PspN4I GGNNCC 4 cut(s) 512, 641, 642, 971
PspPI GGNCC 1 cut(s) 640
PspPPI RGGWCCY 1 cut(s) 640
PsuI RGATCY 2 cut(s) 10, 984
PsyI GACNNNGTC 1 cut(s) 1049
RsaI GTAC 2 cut(s) 512, 941
RsaNI GTAC 2 cut(s) 511, 940
RseI CAYNNNNRTG 1 cut(s) 689
SaqAI TTAA 2 cut(s) 182, 290
SatI GCNGC 2 cut(s) 96, 536
Sau3AI GATC 5 cut(s) 10, 404, 669, 757, 984
Sau96I GGNCC 1 cut(s) 640
SchI GAGTC 1 cut(s) 185
ScrFI CCNGG 4 cut(s) 282, 374, 944, 1059
SduI GDGCHC 1 cut(s) 1185
SfaNI GCATC 4 cut(s) 353, 522, 569, 806
SfuI TTCGAA 2 cut(s) 16, 597
SinI GGWCC 1 cut(s) 640
SmiMI CAYNNNNRTG 1 cut(s) 689
SmlI CTYRAG 1 cut(s) 753
SmoI CTYRAG 1 cut(s) 753
Sse9I AATT 5 cut(s) 198, 358, 546, 735, 877
SspI AATATT 1 cut(s) 209
StyD4I CCNGG 4 cut(s) 280, 372, 942, 1057
TaaI ACNGT 3 cut(s) 688, 892, 1003
TaqI TCGA 3 cut(s) 16, 597, 742
TasI AATT 5 cut(s) 198, 358, 546, 735, 877
TfiI GAWTC 7 cut(s) 23, 146, 240, 434, 526, 599, 723
Tru1I TTAA 2 cut(s) 182, 290
Tru9I TTAA 2 cut(s) 182, 290
TscAI CASTG 4 cut(s) 693, 897, 918, 1008
TseFI GTSAC 4 cut(s) 4, 61, 76, 909
TseI GCWGC 2 cut(s) 95, 535
Tsp45I GTSAC 4 cut(s) 4, 61, 76, 909
TspDTI ATGAA 5 cut(s) 339, 381, 451, 1139, 1191
TspRI CASTG 4 cut(s) 693, 897, 918, 1008
Tth111I GACNNNGTC 1 cut(s) 1049
VpaK11BI GGWCC 1 cut(s) 640
XagI CCTNNNNNAGG 1 cut(s) 1127
XceI RCATGY 1 cut(s) 1069
XmiI GTMKAC 1 cut(s) 866
XmnI GAANNNNTTC 4 cut(s) 22, 738, 1101, 1122
Zsp2I ATGCAT 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.