Rh5AG171500

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001629
Physical Location & Seq
Reverse (-)
18956840 .. 18959154
2315 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG171500.1

Sequence Viewer

Length: 1206 bp
ATGGTGACAGAACTTCGAACAGATTCTTTGGATTACCGCACAGAGCTGTTGTCGCCGGACCTGACAAGAGAGACTGTGACACTGCCAAGTGAGCCGGCTTGGCGGCTCAATATGGATGACTTCAAGCTTCCAGCTGAGAGAAACAACATGGATCCTTTTTTTGGCTTTGGCTCTTTTGTTCAGTCATTACGGACGAAAAGAAAGGTTGCACAGTATTACAGGAGGCAGAACAAACTTCTCAAAGGTTTCAATGAATTAGACTCATTTTCTGAAATGGGTTTTTGGCCTGGAAGTTTAACAAAGGATGAGATGACACAGCTTGAAAGGAGTGAAAGGATAGCAATATATGCATCCAATGCGGCTAATTTGGTGCTCTTCCTAGCAAAAGTGTATGCTTCTATTCAGAGCAGATCATTGGCAGTAATAGCTTCAACTTTGGACTCCCTCTTAGACCTCTTGTCAGGGTTCATCCTTTGGTTTACAGCTTCTGCAATGAGAAAACCAAATCAGTATCACTACCCGATTGGCAAGACTCGGATGCAACCTGTGGGAATTGTTGTTTTTGCATCAATAATGGCAACTCTTGGATTGCAAATTTTGTTTGAATCAGGCCGACAACTTGTCATGAAGACTCAACCTGATAAGGACCCAGAGAAAGAGAAATGGATGATAGGGATAATGGTCTCTGTCACAATAGTCAAGATTGTTCTAATGGTATACTGTCGAAGATTCAAAAACGAAATTGTTCGAGCATATGCTCAAGATCATCTATTTGATGTCATCACTAATGCAATTGGTCTTGCATCAGCAGTGTTAGCCGTCAGGTTTTACTGGTGGATCGATCCAATTGGAGCTATCATCATTGCTTTGTATACAATGGCGAATTGGGCAAAAACAGTGATGGACAATGTGTGGTCACTAATTGGGAAGACAGCACCGGCAGAGTACTTGGCCAAGTTAACATATCTAATTTGGAACCACGACAAGGAGATCAAGCACATTGAGACAGTGAGAGCATACACATTTGGTGTTAACTATTTTGTAGAGGTTCATATAGTCTTACCTGGGGACATGTCTCTCAGTGATGCACATAATATCGGAGAGGCACTCCAAGAAAAGCTTGAGAATCTTCCTGAGGTTGAACGAGCTTTTGTTCATGTTGATGTTGACATTACTCACAAGCCAGAGCACAAGCCAAAGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux ZT_dimer

Protein Analysis

401

Amino Acids

45.84

Weight (kDa)

7.14

Isoelectric Point (pI)

40.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_efflux PF01545 117 - 307 3.3e-33 Cation efflux transmembrane domain
ZT_dimer PF16916 320 - 388 2.5e-10 Cation efflux protein, cytoplasmic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 717, 872
AciI CCGC 3 cut(s) 37, 103, 359
AclWI GGATC 4 cut(s) 146, 159, 836, 845
AcoI YGGCCR 1 cut(s) 951
AcsI RAATTY 1 cut(s) 594
AfaI GTAC 1 cut(s) 947
AfiI CCNNNNNNNGG 2 cut(s) 161, 985
AflIII ACRYGT 1 cut(s) 1071
AgsI TTSAA 7 cut(s) 124, 250, 323, 432, 605, 733, 1142
AhdI GACNNNNNGTC 2 cut(s) 457, 620
AjnI CCWGG 2 cut(s) 286, 1063
AluBI AGCT 9 cut(s) 46, 127, 134, 319, 428, 485, 854, 1120, 1148
AluI AGCT 9 cut(s) 46, 127, 134, 319, 428, 485, 854, 1120, 1148
Alw21I GWGCWC 2 cut(s) 375, 1191
Alw26I GTCTC 4 cut(s) 65, 688, 998, 1080
AlwI GGATC 4 cut(s) 146, 159, 836, 845
AlwNI CAGNNNCTG 1 cut(s) 488
AoxI GGCC 3 cut(s) 284, 610, 951
ApoI RAATTY 1 cut(s) 594
Asp700I GAANNNNTTC 2 cut(s) 22, 744
AspS9I GGNCC 2 cut(s) 58, 646
AsuHPI GGTGA 1 cut(s) 16
AsuII TTCGAA 1 cut(s) 16
AvaII GGWCC 2 cut(s) 58, 646
AxyI CCTNAGG 1 cut(s) 1134
BalI TGGCCA 1 cut(s) 953
BamHI GGATCC 1 cut(s) 151
BbsI GAAGAC 2 cut(s) 635, 935
Bbv12I GWGCWC 2 cut(s) 375, 1191
BccI CCATC 1 cut(s) 895
BceAI ACGGC 1 cut(s) 803
BciT130I CCWGG 2 cut(s) 288, 1065
BcoDI GTCTC 4 cut(s) 65, 688, 998, 1080
BfaI CTAG 1 cut(s) 380
BglI GCCNNNNNGGC 1 cut(s) 100
BisI GCNGC 2 cut(s) 104, 360
BlsI GCNGC 2 cut(s) 105, 361
BmcAI AGTACT 1 cut(s) 947
Bme1390I CCNGG 2 cut(s) 288, 1065
Bme18I GGWCC 2 cut(s) 58, 646
BmeRI GACNNNNNGTC 2 cut(s) 457, 620
BmgT120I GGNCC 2 cut(s) 58, 646
BmiI GGNNCC 3 cut(s) 153, 648, 977
BmrFI CCNGG 2 cut(s) 288, 1065
BmsI GCATC 5 cut(s) 359, 528, 575, 812, 1075
BpiI GAAGAC 2 cut(s) 635, 935
BplI GAGNNNNNCTC 2 cut(s) 1092, 1124
Bpu14I TTCGAA 1 cut(s) 16
BpuEI CTTGAG 2 cut(s) 744, 1142
Bsa29I ATCGAT 1 cut(s) 840
BsaI GGTCTC 1 cut(s) 688
BsaJI CCNNGG 1 cut(s) 1064
Bsc4I CCNNNNNNNGG 2 cut(s) 161, 985
Bse118I RCCGGY 2 cut(s) 94, 937
Bse1I ACTGG 1 cut(s) 836
Bse21I CCTNAGG 1 cut(s) 1134
Bse3DI GCAATG 2 cut(s) 498, 861
BseBI CCWGG 2 cut(s) 288, 1065
BseCI ATCGAT 1 cut(s) 840
BseDI CCNNGG 1 cut(s) 1064
BseGI GGATG 6 cut(s) 121, 310, 350, 468, 543, 672
BseLI CCNNNNNNNGG 2 cut(s) 161, 985
BseMI GCAATG 2 cut(s) 498, 861
BseMII CTCAG 3 cut(s) 126, 1093, 1125
BseNI ACTGG 1 cut(s) 836
BshFI GGCC 3 cut(s) 286, 612, 953
BshVI ATCGAT 1 cut(s) 840
BsiHKAI GWGCWC 2 cut(s) 375, 1191
BsiSI CCGG 3 cut(s) 56, 95, 938
BslFI GGGAC 1 cut(s) 1082
BslI CCNNNNNNNGG 2 cut(s) 161, 985
BsmAI GTCTC 4 cut(s) 65, 688, 998, 1080
BsmFI GGGAC 1 cut(s) 1082
BsnI GGCC 3 cut(s) 286, 612, 953
Bso31I GGTCTC 1 cut(s) 688
Bsp119I TTCGAA 1 cut(s) 16
Bsp1286I GDGCHC 2 cut(s) 375, 1191
Bsp143I GATC 6 cut(s) 151, 410, 763, 837, 841, 990
BspACI CCGC 3 cut(s) 37, 103, 359
BspANI GGCC 3 cut(s) 286, 612, 953
BspCNI CTCAG 3 cut(s) 127, 1092, 1126
BspDI ATCGAT 1 cut(s) 840
BspHI TCATGA 1 cut(s) 624
BspLI GGNNCC 3 cut(s) 153, 648, 977
BspPI GGATC 4 cut(s) 146, 159, 836, 845
BspQI GCTCTTC 1 cut(s) 380
BspT104I TTCGAA 1 cut(s) 16
BspTNI GGTCTC 1 cut(s) 688
BsrDI GCAATG 2 cut(s) 498, 861
BsrFI RCCGGY 2 cut(s) 94, 937
BsrI ACTGG 1 cut(s) 836
BssAI RCCGGY 2 cut(s) 94, 937
BssECI CCNNGG 1 cut(s) 1064
BssMI GATC 6 cut(s) 151, 410, 763, 837, 841, 990
BssNAI GTATAC 2 cut(s) 718, 873
Bst1107I GTATAC 2 cut(s) 718, 873
Bst2UI CCWGG 2 cut(s) 288, 1065
Bst4CI ACNGT 5 cut(s) 76, 213, 722, 898, 1009
Bst6I CTCTTC 1 cut(s) 380
BstAPI GCANNNNNTGC 2 cut(s) 347, 356
BstBI TTCGAA 1 cut(s) 16
BstC8I GCNNGC 1 cut(s) 96
BstDEI CTNAG 4 cut(s) 135, 448, 1079, 1134
BstF5I GGATG 6 cut(s) 121, 310, 350, 468, 543, 672
BstKTI GATC 6 cut(s) 154, 413, 766, 840, 844, 993
BstMAI GTCTC 4 cut(s) 65, 688, 998, 1080
BstMBI GATC 6 cut(s) 151, 410, 763, 837, 841, 990
BstMWI GCNNNNNNNGC 8 cut(s) 52, 91, 100, 347, 356, 425, 815, 887
BstNI CCWGG 2 cut(s) 288, 1065
BstNSI RCATGY 1 cut(s) 1075
BstSCI CCNGG 2 cut(s) 286, 1063
BstV2I GAAGAC 2 cut(s) 635, 935
BstX2I RGATCY 1 cut(s) 151
BstYI RGATCY 1 cut(s) 151
BstZ17I GTATAC 2 cut(s) 718, 873
Bsu15I ATCGAT 1 cut(s) 840
Bsu36I CCTNAGG 1 cut(s) 1134
BsuRI GGCC 3 cut(s) 286, 612, 953
BsuTUI ATCGAT 1 cut(s) 840
BtsCI GGATG 6 cut(s) 121, 310, 350, 468, 543, 672
BtsI GCAGTG 2 cut(s) 80, 816
BtsIMutI CAGTG 5 cut(s) 80, 816, 903, 1014, 1087
Cac8I GCNNGC 1 cut(s) 96
CaiI CAGNNNCTG 1 cut(s) 488
CciI TCATGA 1 cut(s) 624
Cfr10I RCCGGY 2 cut(s) 94, 937
Cfr13I GGNCC 2 cut(s) 58, 646
ClaI ATCGAT 1 cut(s) 840
Csp6I GTAC 1 cut(s) 946
CviAII CATG 4 cut(s) 148, 625, 1072, 1157
CviQI GTAC 1 cut(s) 946
DdeI CTNAG 4 cut(s) 135, 448, 1079, 1134
DpnI GATC 6 cut(s) 153, 412, 765, 839, 843, 992
DpnII GATC 6 cut(s) 151, 410, 763, 837, 841, 990
DriI GACNNNNNGTC 2 cut(s) 457, 620
EaeI YGGCCR 1 cut(s) 951
Eam1104I CTCTTC 1 cut(s) 380
Eam1105I GACNNNNNGTC 2 cut(s) 457, 620
EarI CTCTTC 1 cut(s) 380
Eco31I GGTCTC 1 cut(s) 688
Eco47I GGWCC 2 cut(s) 58, 646
Eco81I CCTNAGG 1 cut(s) 1134
EcoO109I RGGNCCY 1 cut(s) 646
EcoRII CCWGG 2 cut(s) 286, 1063
EcoT22I ATGCAT 1 cut(s) 352
FaeI CATG 4 cut(s) 151, 628, 1075, 1160
FalI AAGNNNNNCTT 2 cut(s) 1104, 1136
FaqI GGGAC 1 cut(s) 1082
FatI CATG 4 cut(s) 147, 624, 1071, 1156
FauNDI CATATG 1 cut(s) 754
FblI GTMKAC 2 cut(s) 717, 872
Fnu4HI GCNGC 2 cut(s) 104, 360
FokI GGATG 6 cut(s) 128, 317, 337, 455, 550, 679
Fsp4HI GCNGC 2 cut(s) 104, 360
FspBI CTAG 1 cut(s) 380
GluI GCNGC 2 cut(s) 104, 360
HaeIII GGCC 3 cut(s) 286, 612, 953
HapII CCGG 3 cut(s) 56, 95, 938
Hin1II CATG 4 cut(s) 151, 628, 1075, 1160
HincII GTYRAC 3 cut(s) 960, 1033, 1168
HindII GTYRAC 3 cut(s) 960, 1033, 1168
HindIII AAGCTT 2 cut(s) 125, 1118
HinfI GANTC 8 cut(s) 23, 260, 440, 532, 605, 631, 729, 1126
HpaI GTTAAC 2 cut(s) 960, 1033
HpaII CCGG 3 cut(s) 56, 95, 938
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 6 cut(s) 480, 718, 873, 960, 1033, 1168
Hpy188I TCNGA 4 cut(s) 271, 405, 537, 1100
Hpy188III TCNNGA 4 cut(s) 625, 700, 761, 1133
Hpy8I GTNNAC 6 cut(s) 480, 718, 873, 960, 1033, 1168
HpyCH4III ACNGT 5 cut(s) 76, 213, 722, 898, 1009
HpyCH4V TGCA 9 cut(s) 209, 350, 491, 541, 566, 592, 791, 803, 1088
HpyF10VI GCNNNNNNNGC 8 cut(s) 52, 91, 100, 347, 356, 425, 815, 887
HpyF3I CTNAG 4 cut(s) 135, 448, 1079, 1134
Hsp92II CATG 4 cut(s) 151, 628, 1075, 1160
KroI GCCGGC 1 cut(s) 94
KroNI GCCGGC 1 cut(s) 96
KspAI GTTAAC 2 cut(s) 960, 1033
Kzo9I GATC 6 cut(s) 151, 410, 763, 837, 841, 990
LguI GCTCTTC 1 cut(s) 380
LmnI GCTCC 1 cut(s) 851
LweI GCATC 5 cut(s) 359, 528, 575, 812, 1075
MaeI CTAG 1 cut(s) 380
MaeIII GTNAC 4 cut(s) 4, 76, 688, 915
MalI GATC 6 cut(s) 153, 412, 765, 839, 843, 992
MboI GATC 6 cut(s) 151, 410, 763, 837, 841, 990
MboII GAAGA 5 cut(s) 367, 640, 738, 940, 1121
MfeI CAATTG 2 cut(s) 792, 846
MflI RGATCY 1 cut(s) 151
MhlI GDGCHC 2 cut(s) 375, 1191
MlsI TGGCCA 1 cut(s) 953
MluNI TGGCCA 1 cut(s) 953
MlyI GAGTC 4 cut(s) 254, 434, 526, 625
MnlI CCTC 6 cut(s) 216, 455, 464, 1039, 1096, 1129
Mox20I TGGCCA 1 cut(s) 953
Mph1103I ATGCAT 1 cut(s) 352
MroNI GCCGGC 1 cut(s) 94
MroXI GAANNNNTTC 2 cut(s) 22, 744
MscI TGGCCA 1 cut(s) 953
MseI TTAA 3 cut(s) 296, 959, 1032
MslI CAYNNNNRTG 1 cut(s) 1161
Msp20I TGGCCA 1 cut(s) 953
MspA1I CMGCKG 1 cut(s) 134
MspI CCGG 3 cut(s) 56, 95, 938
MspR9I CCNGG 2 cut(s) 288, 1065
MunI CAATTG 2 cut(s) 792, 846
MvaI CCWGG 2 cut(s) 288, 1065
MwoI GCNNNNNNNGC 8 cut(s) 52, 91, 100, 347, 356, 425, 815, 887
NaeI GCCGGC 1 cut(s) 96
NdeI CATATG 1 cut(s) 754
NdeII GATC 6 cut(s) 151, 410, 763, 837, 841, 990
NgoMIV GCCGGC 1 cut(s) 94
NlaIII CATG 4 cut(s) 151, 628, 1075, 1160
NlaIV GGNNCC 3 cut(s) 153, 648, 977
NmuCI GTSAC 4 cut(s) 4, 76, 688, 915
NsiI ATGCAT 1 cut(s) 352
NspI RCATGY 1 cut(s) 1075
NspV TTCGAA 1 cut(s) 16
PagI TCATGA 1 cut(s) 624
PciI ACATGT 1 cut(s) 1071
PciSI GCTCTTC 1 cut(s) 380
PdiI GCCGGC 1 cut(s) 96
PdmI GAANNNNTTC 2 cut(s) 22, 744
PfeI GAWTC 4 cut(s) 23, 605, 729, 1126
PkrI GCNGC 2 cut(s) 105, 361
PleI GAGTC 4 cut(s) 254, 434, 526, 625
PpsI GAGTC 4 cut(s) 254, 434, 526, 625
PpuMI RGGWCCY 1 cut(s) 646
PscI ACATGT 1 cut(s) 1071
Psp5II RGGWCCY 1 cut(s) 646
Psp6I CCWGG 2 cut(s) 286, 1063
PspGI CCWGG 2 cut(s) 286, 1063
PspN4I GGNNCC 3 cut(s) 153, 648, 977
PspPI GGNCC 2 cut(s) 58, 646
PspPPI RGGWCCY 1 cut(s) 646
PstNI CAGNNNCTG 1 cut(s) 488
PsuI RGATCY 1 cut(s) 151
PvuII CAGCTG 1 cut(s) 134
RsaI GTAC 1 cut(s) 947
RsaNI GTAC 1 cut(s) 946
RseI CAYNNNNRTG 1 cut(s) 1161
SapI GCTCTTC 1 cut(s) 380
SaqAI TTAA 3 cut(s) 296, 959, 1032
SatI GCNGC 2 cut(s) 104, 360
Sau3AI GATC 6 cut(s) 151, 410, 763, 837, 841, 990
Sau96I GGNCC 2 cut(s) 58, 646
ScaI AGTACT 1 cut(s) 947
SchI GAGTC 4 cut(s) 254, 434, 526, 625
ScrFI CCNGG 2 cut(s) 288, 1065
SduI GDGCHC 2 cut(s) 375, 1191
SfaNI GCATC 5 cut(s) 359, 528, 575, 812, 1075
SfuI TTCGAA 1 cut(s) 16
SinI GGWCC 2 cut(s) 58, 646
SmiMI CAYNNNNRTG 1 cut(s) 1161
SmlI CTYRAG 2 cut(s) 759, 1121
SmoI CTYRAG 2 cut(s) 759, 1121
SsiI CCGC 3 cut(s) 37, 103, 359
SspMI CTAG 1 cut(s) 380
StyD4I CCNGG 2 cut(s) 286, 1063
TaaI ACNGT 5 cut(s) 76, 213, 722, 898, 1009
TaqI TCGA 4 cut(s) 16, 724, 748, 840
TatI WGTACW 1 cut(s) 945
TauI GCSGC 2 cut(s) 106, 362
TfiI GAWTC 4 cut(s) 23, 605, 729, 1126
Tru1I TTAA 3 cut(s) 296, 959, 1032
Tru9I TTAA 3 cut(s) 296, 959, 1032
TscAI CASTG 5 cut(s) 87, 816, 903, 1014, 1087
TseFI GTSAC 4 cut(s) 4, 76, 688, 915
Tsp45I GTSAC 4 cut(s) 4, 76, 688, 915
TspDTI ATGAA 5 cut(s) 267, 457, 641, 1040, 1145
TspGWI ACGGA 1 cut(s) 205
TspRI CASTG 5 cut(s) 87, 816, 903, 1014, 1087
VpaK11BI GGWCC 2 cut(s) 58, 646
XapI RAATTY 1 cut(s) 594
XceI RCATGY 1 cut(s) 1075
XmiI GTMKAC 2 cut(s) 717, 872
XmnI GAANNNNTTC 2 cut(s) 22, 744
XspI CTAG 1 cut(s) 380
ZrmI AGTACT 1 cut(s) 947
Zsp2I ATGCAT 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.