Rorug05G0081100

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001629
Physical Location & Seq
Forward (+)
7019119 .. 7023312
4194 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0081100.1

Sequence Viewer

Length: 2109 bp
ATGGAGGGGAGGAAACTGAATTTCAGTGCACCACTTCTGTCAGTAAGACGAATTTCTGCAACTCCAGTGTCCACGAATGCAGATACTGGGAAGGTAACTCCTAGGTTGGCTAATGGAAGACATACTCTTCCTCTTCCCAAATCAGATTTTTCTTTGGAGCAGGTAACAAAACCAGTTGCAGTTCCGTTTCATTGGGAGGACATCCCAGGAAGATCTAAGGAAGGTGAAGAACCGAAGATTCAGTCTCGAGAAGAGGCTTCTGTTACTCCAAGACTTCCCCCTGGAAAGATTGTAGAGGGTAAACAGAAACCTTTGGAAAAAGAGCGAGCTGATCAAAAGGCTATCACACCCTACTTGAAATCATATTCTTCGAATGAAAATGCCATGGAAGCCAAGTGCTCAAAAGAGAGAGTGATTGAGAGAAGAGCTTCAAAATTTGTGGATGAGGATGATGTTTATTCTGATGCACAAGAGACACTGTCCCCGCCTACAGAGTCTTTCTCCATGAACTGTAGTGCAACTGGCACAAACCAATCTCATTCTGCAAATGTGCAGCCATCTATTGATCCACAAACTCGAGACTTCATGATGAAGCGATTCTTACCTGCAGCCAAGGCAATGGCTTTGGAGACGCCTCATGTTGCTATAAAGAAGCAGCAATATTCAGCACCACTTGAACAATCAAGACAAGTCACAAGGGTAATTCGCCAGGATACGAGTCCCTTACCTAATCAACAACGGTCTCACATGGAACTAGTGCTGTATAGCCAGTACAAAGAGGAAGAAGAAAGTGAAGGAGAAGATGATGAGTATGATCAGCCTGGTTATATTTCACCAAAGGGTTGTGGGTTGTTTCCTCGGTTAGGCTTAAGAAATTCTTTGTGCCCTTTACCTGTTCCGGCAATGAAAGCTAGGGCCCGGGCTCTCATGTCTTGCTCCTCTAGTGTTGCAATACCAGGAAAAACTACTCGTAGTCGATCTCACAGTCAACCACTCAAGCAGGTTATCAAGACTACCAATAGACAGTCACATAGCCAACCACTCAATGAGGTTATTAAGCCTGCTAAGATCCAGTCTCATAACCAACCACTCAACAAGGTTATTAAGCCTGCTAATAGTCAGTCTCATTGCCAACCACTCAATAAGCGTGATCAGGGTGCTGCTTATAATCTCAAAGGAGACCGTGGATTTTATTCAGGTGAATTGCCGAGATTGATGAATAATCAGGCTGCTCAATCCATTCAGTTTAGGCACTCTGGTGAGCAACCAAAAGGCAGGTCATCTCCATTCAGGCAATCAAGAAGTCCTTGCATATCCCCGTATCGAAATGAACGGCCTAAATCTCCCTTTACTGGTGCGGGGTTTCTTGGCGTTCCCAAAGAAGAAAATGTCAAAGCCAATAGTAGTAAGTTCAATATGAACGACAAAGTTGGTCACAAGTCTCAGGAGGTACTAAGCCATCAAAGAAATAAACAAGGGCCATATACTGTGATCCCTATGATTGAGAAGACACTATATGTAGATACTGTAAATACTACCAGAACATCGTGTCTAAATTCAAGCTCTTTGTATACCAAAGAACGAGTCGACTCTGCTGTTGAGGACTTAAGGACCCTATTAAAGAGAAGTGGGATGGAAGAAACGTCTGCCGGAGAGTCTTTGTTTCAAGATATCAAATGCCTGACTAATTTTGAGAGCAATGGCATCTCGGAACCTGCAGCATTGGAGTCTGTTGAAGATAGTTTATCTTCATTCTCCGACATATCCCATATCAAAGGCCAAGTAGTTGCAGTGGGACTTGATATGAGAAGTGATGACATTATGAAAGAGGATCCACGAAATGCCAACACCATCGTGGTACAGTCTTCTCTTCCCCCACCTTTACCAAAATCACCTTCCCAGTCTTGGCTCTCATCGACCCTCTCTTCAATTCCTTCTCGGAATTCAGTCTCCTCTGGTACCAAAAGGCATACTAAAAGGTCTGATTCCAAGACTTCTTCCACTGGCACCAAGTGGGAAACCATTGTAAAGACTTCCAATTTGCACCATGATCATGTGCGCTATTCCGAGGAATTAATTACTCATGCTTGTCAGCAATCTAAGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux ZT_dimer

Protein Analysis

702

Amino Acids

77.98

Weight (kDa)

9.3

Isoelectric Point (pI)

59.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF688 PF05097 1 - 366 8.6e-70 Protein of unknown function (DUF688)
DUF688 PF05097 410 - 528 2.1e-11 Protein of unknown function (DUF688)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1167
Acc36I ACCTGC 5 cut(s) 151, 613, 991, 1266, 1723
Acc65I GGTACC 1 cut(s) 1958
AccB1I GGYRCC 2 cut(s) 1958, 2006
AccI GTMKAC 2 cut(s) 1571, 1587
AciI CCGC 2 cut(s) 485, 1358
AclWI GGATC 5 cut(s) 560, 1063, 1486, 1826, 1839
AcsI RAATTY 6 cut(s) 19, 51, 434, 874, 1555, 1942
AcyI GRCGYC 1 cut(s) 632
AdeI CACNNNGTG 1 cut(s) 2013
AfaI GTAC 4 cut(s) 773, 1452, 1860, 1960
AfiI CCNNNNNNNGG 3 cut(s) 863, 1352, 1905
AflII CTTAAG 2 cut(s) 868, 1606
AgsI TTSAA 8 cut(s) 358, 432, 677, 1414, 1560, 1667, 1736, 1929
AhlI ACTAGT 1 cut(s) 754
AjnI CCWGG 5 cut(s) 205, 280, 708, 820, 955
AjuI GAANNNNNNNTTGG 2 cut(s) 1879, 1911
AleI CACNNNNGTG 2 cut(s) 1257, 1853
AluBI AGCT 4 cut(s) 329, 428, 911, 1563
AluI AGCT 4 cut(s) 329, 428, 911, 1563
Alw21I GWGCWC 2 cut(s) 31, 401
Alw44I GTGCAC 1 cut(s) 27
AlwI GGATC 5 cut(s) 560, 1063, 1486, 1826, 1839
AlwNI CAGNNNCTG 1 cut(s) 86
Ama87I CYCGRG 3 cut(s) 246, 576, 918
AoxI GGCC 4 cut(s) 915, 1334, 1478, 1777
ApaI GGGCCC 1 cut(s) 919
ApaLI GTGCAC 1 cut(s) 27
ApeKI GCWGC 6 cut(s) 553, 608, 655, 1160, 1229, 1718
ApoI RAATTY 6 cut(s) 19, 51, 434, 874, 1555, 1942
ArsI GACNNNNNNTTYG 8 cut(s) 227, 259, 1262, 1294, 1569, 1601, 2023, 2055
AseI ATTAAT 1 cut(s) 2075
Asp700I GAANNNNTTC 2 cut(s) 427, 596
Asp718I GGTACC 1 cut(s) 1958
AspA2I CCTAGG 1 cut(s) 101
AspLEI GCGC 1 cut(s) 2061
AspS9I GGNCC 4 cut(s) 915, 916, 1478, 1611
AsuC2I CCSGG 2 cut(s) 919, 920
AsuHPI GGTGA 5 cut(s) 236, 825, 1211, 1271, 1884
AsuII TTCGAA 1 cut(s) 371
AvaI CYCGRG 3 cut(s) 246, 576, 918
AvaII GGWCC 1 cut(s) 1611
AvrII CCTAGG 1 cut(s) 101
BaeGI GKGCMC 3 cut(s) 31, 887, 919
BamHI GGATCC 1 cut(s) 1831
BanI GGYRCC 2 cut(s) 1958, 2006
BanII GRGCYC 2 cut(s) 919, 925
BbsI GAAGAC 3 cut(s) 124, 1514, 1857
Bbv12I GWGCWC 2 cut(s) 31, 401
BbvI GCAGC 6 cut(s) 565, 620, 667, 1147, 1216, 1730
BccI CCATC 4 cut(s) 565, 1467, 1627, 1859
BceAI ACGGC 1 cut(s) 1349
BciT130I CCWGG 5 cut(s) 207, 282, 710, 822, 957
BciVI GTATCC 1 cut(s) 706
BclI TGATCA 4 cut(s) 331, 814, 1150, 2050
BcnI CCSGG 2 cut(s) 919, 920
BcuI ACTAGT 1 cut(s) 754
BfaI CTAG 4 cut(s) 102, 755, 912, 942
BfmI CTRYAG 4 cut(s) 489, 511, 606, 1716
BfrI CTTAAG 2 cut(s) 868, 1606
BfuAI ACCTGC 5 cut(s) 151, 613, 991, 1266, 1723
BfuI GTATCC 1 cut(s) 706
BglII AGATCT 1 cut(s) 212
BisI GCNGC 6 cut(s) 554, 609, 656, 1161, 1230, 1719
BlnI CCTAGG 1 cut(s) 101
BlsI GCNGC 6 cut(s) 555, 610, 657, 1162, 1231, 1720
Bme1390I CCNGG 7 cut(s) 207, 282, 710, 822, 919, 920, 957
Bme18I GGWCC 1 cut(s) 1611
BmeT110I CYCGRG 3 cut(s) 246, 576, 918
BmgT120I GGNCC 4 cut(s) 915, 916, 1478, 1611
BmiI GGNNCC 6 cut(s) 917, 1613, 1713, 1833, 1960, 2008
BmrFI CCNGG 7 cut(s) 207, 282, 710, 822, 919, 920, 957
BmrI ACTGGG 2 cut(s) 96, 1894
BmsI GCATC 2 cut(s) 454, 1713
BmuI ACTGGG 2 cut(s) 96, 1894
BpiI GAAGAC 3 cut(s) 124, 1514, 1857
BplI GAGNNNNNCTC 2 cut(s) 485, 517
BpmI CTGGAG 1 cut(s) 48
Bpu14I TTCGAA 1 cut(s) 371
BpuEI CTTGAG 1 cut(s) 980
BpuMI CCSGG 2 cut(s) 919, 920
BsaHI GRCGYC 1 cut(s) 632
BsaI GGTCTC 2 cut(s) 747, 1173
BsaJI CCNNGG 9 cut(s) 101, 205, 280, 384, 612, 857, 918, 1183, 2067
Bsc4I CCNNNNNNNGG 3 cut(s) 863, 1352, 1905
Bse1I ACTGG 9 cut(s) 65, 91, 173, 526, 769, 1072, 1357, 1900, 2008
Bse3DI GCAATG 4 cut(s) 624, 909, 1126, 1705
BseBI CCWGG 5 cut(s) 207, 282, 710, 822, 957
BseDI CCNNGG 9 cut(s) 101, 205, 280, 384, 612, 857, 918, 1183, 2067
BseGI GGATG 4 cut(s) 201, 448, 454, 1638
BseLI CCNNNNNNNGG 3 cut(s) 863, 1352, 1905
BseMI GCAATG 4 cut(s) 624, 909, 1126, 1705
BseMII CTCAG 1 cut(s) 1457
BseNI ACTGG 9 cut(s) 65, 91, 173, 526, 769, 1072, 1357, 1900, 2008
BseRI GAGGAG 2 cut(s) 928, 1942
BseSI GKGCMC 3 cut(s) 31, 887, 919
BseXI GCAGC 6 cut(s) 565, 620, 667, 1147, 1216, 1730
BsgI GTGCAG 1 cut(s) 572
BshFI GGCC 4 cut(s) 917, 1336, 1480, 1779
BshNI GGYRCC 2 cut(s) 1958, 2006
BsiHKAI GWGCWC 2 cut(s) 31, 401
BsiHKCI CYCGRG 3 cut(s) 246, 576, 918
BsiSI CCGG 3 cut(s) 899, 919, 1650
BslFI GGGAC 3 cut(s) 466, 705, 1809
BslI CCNNNNNNNGG 3 cut(s) 863, 1352, 1905
BsmBI CGTCTC 1 cut(s) 623
BsmFI GGGAC 3 cut(s) 466, 705, 1809
BsmI GAATGC 1 cut(s) 82
BsnI GGCC 4 cut(s) 917, 1336, 1480, 1779
Bso31I GGTCTC 2 cut(s) 747, 1173
BsoBI CYCGRG 3 cut(s) 246, 576, 918
Bsp119I TTCGAA 1 cut(s) 371
Bsp120I GGGCCC 1 cut(s) 915
Bsp1286I GDGCHC 5 cut(s) 31, 401, 887, 919, 925
Bsp19I CCATGG 1 cut(s) 384
BspACI CCGC 2 cut(s) 485, 1358
BspANI GGCC 4 cut(s) 917, 1336, 1480, 1779
BspCNI CTCAG 1 cut(s) 1456
BspHI TCATGA 1 cut(s) 585
BspLI GGNNCC 6 cut(s) 917, 1613, 1713, 1833, 1960, 2008
BspMAI CTGCAG 2 cut(s) 610, 1720
BspMI ACCTGC 5 cut(s) 151, 613, 991, 1266, 1723
BspPI GGATC 5 cut(s) 560, 1063, 1486, 1826, 1839
BspQI GCTCTTC 1 cut(s) 418
BspT104I TTCGAA 1 cut(s) 371
BspT107I GGYRCC 2 cut(s) 1958, 2006
BspTI CTTAAG 2 cut(s) 868, 1606
BspTNI GGTCTC 2 cut(s) 747, 1173
BsrDI GCAATG 4 cut(s) 624, 909, 1126, 1705
BsrI ACTGG 9 cut(s) 65, 91, 173, 526, 769, 1072, 1357, 1900, 2008
BssECI CCNNGG 9 cut(s) 101, 205, 280, 384, 612, 857, 918, 1183, 2067
BssNAI GTATAC 1 cut(s) 1572
BssNI GRCGYC 1 cut(s) 632
BssT1I CCWWGG 3 cut(s) 101, 384, 612
Bst1107I GTATAC 1 cut(s) 1572
Bst2UI CCWGG 5 cut(s) 207, 282, 710, 822, 957
Bst4CI ACNGT 9 cut(s) 480, 512, 741, 986, 1026, 1184, 1489, 1528, 1863
Bst6I CTCTTC 6 cut(s) 132, 138, 246, 418, 1875, 1930
BstACI GRCGYC 1 cut(s) 632
BstAFI CTTAAG 2 cut(s) 868, 1606
BstBI TTCGAA 1 cut(s) 371
BstC8I GCNNGC 3 cut(s) 327, 1062, 1110
BstDEI CTNAG 5 cut(s) 216, 1065, 1443, 1454, 2100
BstDSI CCRYGG 2 cut(s) 384, 1183
BstENI CCTNNNNNAGG 1 cut(s) 861
BstF5I GGATG 4 cut(s) 201, 448, 454, 1638
BstHHI GCGC 1 cut(s) 2061
BstMWI GCNNNNNNNGC 3 cut(s) 389, 614, 908
BstNI CCWGG 5 cut(s) 207, 282, 710, 822, 957
BstSCI CCNGG 7 cut(s) 205, 280, 708, 820, 917, 918, 955
BstSFI CTRYAG 4 cut(s) 489, 511, 606, 1716
BstSLI GKGCMC 3 cut(s) 31, 887, 919
BstV1I GCAGC 6 cut(s) 565, 620, 667, 1147, 1216, 1730
BstV2I GAAGAC 3 cut(s) 124, 1514, 1857
BstX2I RGATCY 3 cut(s) 212, 1068, 1831
BstXI CCANNNNNNTGG 1 cut(s) 619
BstYI RGATCY 3 cut(s) 212, 1068, 1831
BstZ17I GTATAC 1 cut(s) 1572
BsuI GTATCC 1 cut(s) 706
BsuRI GGCC 4 cut(s) 917, 1336, 1480, 1779
BtgI CCRYGG 2 cut(s) 384, 1183
BtsCI GGATG 4 cut(s) 201, 448, 454, 1638
BtsI GCAGTG 1 cut(s) 1797
BtsIMutI CAGTG 5 cut(s) 31, 72, 476, 1797, 2001
BveI ACCTGC 5 cut(s) 151, 613, 991, 1266, 1723
Cac8I GCNNGC 3 cut(s) 327, 1062, 1110
CaiI CAGNNNCTG 1 cut(s) 86
CciI TCATGA 1 cut(s) 585
CfoI GCGC 1 cut(s) 2061
Cfr13I GGNCC 4 cut(s) 915, 916, 1478, 1611
Cfr9I CCCGGG 1 cut(s) 918
CseI GACGC 1 cut(s) 640
Csp6I GTAC 4 cut(s) 772, 1451, 1859, 1959
CspCI CAANNNNNGTGG 2 cut(s) 420, 455
CviAII CATG 9 cut(s) 385, 505, 586, 638, 748, 928, 2048, 2054, 2084
CviQI GTAC 4 cut(s) 772, 1451, 1859, 1959
DdeI CTNAG 5 cut(s) 216, 1065, 1443, 1454, 2100
DraIII CACNNNGTG 1 cut(s) 2013
Eam1104I CTCTTC 6 cut(s) 132, 138, 246, 418, 1875, 1930
EarI CTCTTC 6 cut(s) 132, 138, 246, 418, 1875, 1930
Eco130I CCWWGG 3 cut(s) 101, 384, 612
Eco24I GRGCYC 2 cut(s) 919, 925
Eco31I GGTCTC 2 cut(s) 747, 1173
Eco32I GATATC 1 cut(s) 1672
Eco47I GGWCC 1 cut(s) 1611
Eco88I CYCGRG 3 cut(s) 246, 576, 918
EcoNI CCTNNNNNAGG 1 cut(s) 861
EcoO109I RGGNCCY 2 cut(s) 915, 1611
EcoRI GAATTC 1 cut(s) 1942
EcoRII CCWGG 5 cut(s) 205, 280, 708, 820, 955
EcoRV GATATC 1 cut(s) 1672
EcoT14I CCWWGG 3 cut(s) 101, 384, 612
EcoT38I GRGCYC 2 cut(s) 919, 925
ErhI CCWWGG 3 cut(s) 101, 384, 612
Esp3I CGTCTC 1 cut(s) 623
FaeI CATG 9 cut(s) 388, 508, 589, 641, 751, 931, 2051, 2057, 2087
FalI AAGNNNNNCTT 6 cut(s) 584, 616, 862, 894, 1291, 1323
FaqI GGGAC 3 cut(s) 466, 705, 1809
FatI CATG 9 cut(s) 384, 504, 585, 637, 747, 927, 2047, 2053, 2083
FauI CCCGC 2 cut(s) 492, 1351
FbaI TGATCA 4 cut(s) 331, 814, 1150, 2050
FblI GTMKAC 2 cut(s) 1571, 1587
Fnu4HI GCNGC 6 cut(s) 554, 609, 656, 1161, 1230, 1719
FokI GGATG 4 cut(s) 188, 455, 461, 1645
FriOI GRGCYC 2 cut(s) 919, 925
Fsp4HI GCNGC 6 cut(s) 554, 609, 656, 1161, 1230, 1719
FspBI CTAG 4 cut(s) 102, 755, 912, 942
GlaI GCGC 1 cut(s) 2060
GluI GCNGC 6 cut(s) 554, 609, 656, 1161, 1230, 1719
GsuI CTGGAG 1 cut(s) 48
HaeIII GGCC 4 cut(s) 917, 1336, 1480, 1779
HapII CCGG 3 cut(s) 899, 919, 1650
HgaI GACGC 1 cut(s) 640
HhaI GCGC 1 cut(s) 2061
Hin1I GRCGYC 1 cut(s) 632
Hin1II CATG 9 cut(s) 388, 508, 589, 641, 751, 931, 2051, 2057, 2087
Hin6I GCGC 1 cut(s) 2059
HinP1I GCGC 1 cut(s) 2059
HincII GTYRAC 2 cut(s) 989, 1588
HindII GTYRAC 2 cut(s) 989, 1588
HinfI GANTC 9 cut(s) 238, 494, 597, 718, 1584, 1589, 1655, 1727, 1985
HpaII CCGG 3 cut(s) 899, 919, 1650
HphI GGTGA 5 cut(s) 236, 825, 1211, 1271, 1884
Hpy166II GTNNAC 6 cut(s) 29, 72, 302, 989, 1572, 1588
Hpy188I TCNGA 7 cut(s) 145, 463, 1711, 1759, 1941, 1984, 2068
Hpy188III TCNNGA 9 cut(s) 246, 248, 578, 586, 684, 1009, 1299, 1445, 1667
Hpy8I GTNNAC 6 cut(s) 29, 72, 302, 989, 1572, 1588
HpyAV CCTTC 5 cut(s) 85, 215, 788, 1905, 1944
HpyCH4III ACNGT 9 cut(s) 480, 512, 741, 986, 1026, 1184, 1489, 1528, 1863
HpyCH4IV ACGT 1 cut(s) 1643
HpyF10VI GCNNNNNNNGC 3 cut(s) 389, 614, 908
HpyF3I CTNAG 5 cut(s) 216, 1065, 1443, 1454, 2100
HpySE526I ACGT 1 cut(s) 1643
Hsp92I GRCGYC 1 cut(s) 632
Hsp92II CATG 9 cut(s) 388, 508, 589, 641, 751, 931, 2051, 2057, 2087
HspAI GCGC 1 cut(s) 2059
KpnI GGTACC 1 cut(s) 1962
Ksp22I TGATCA 4 cut(s) 331, 814, 1150, 2050
LguI GCTCTTC 1 cut(s) 418
LmnI GCTCC 2 cut(s) 157, 941
Lsp1109I GCAGC 6 cut(s) 565, 620, 667, 1147, 1216, 1730
LweI GCATC 2 cut(s) 454, 1713
MaeI CTAG 4 cut(s) 102, 755, 912, 942
MaeII ACGT 1 cut(s) 1643
MaeIII GTNAC 6 cut(s) 94, 163, 262, 691, 1026, 1433
MflI RGATCY 3 cut(s) 212, 1068, 1831
MhlI GDGCHC 5 cut(s) 31, 401, 887, 919, 925
MlyI GAGTC 6 cut(s) 503, 727, 1583, 1593, 1664, 1736
MmeI TCCRAC 1 cut(s) 1782
MroXI GAANNNNTTC 2 cut(s) 427, 596
MseI TTAA 7 cut(s) 869, 1056, 1104, 1607, 1619, 2075, 2107
MslI CAYNNNNRTG 3 cut(s) 1257, 1853, 2052
MspCI CTTAAG 2 cut(s) 868, 1606
MspI CCGG 3 cut(s) 899, 919, 1650
MspR9I CCNGG 7 cut(s) 207, 282, 710, 822, 919, 920, 957
Mva1269I GAATGC 1 cut(s) 82
MvaI CCWGG 5 cut(s) 207, 282, 710, 822, 957
MwoI GCNNNNNNNGC 3 cut(s) 389, 614, 908
NciI CCSGG 2 cut(s) 919, 920
NcoI CCATGG 1 cut(s) 384
NlaIII CATG 9 cut(s) 388, 508, 589, 641, 751, 931, 2051, 2057, 2087
NlaIV GGNNCC 6 cut(s) 917, 1613, 1713, 1833, 1960, 2008
NmeAIII GCCGAG 1 cut(s) 1233
NmuCI GTSAC 3 cut(s) 691, 1026, 1433
NspV TTCGAA 1 cut(s) 371
OliI CACNNNNGTG 2 cut(s) 1257, 1853
PaeR7I CTCGAG 2 cut(s) 246, 576
PagI TCATGA 1 cut(s) 585
PciSI GCTCTTC 1 cut(s) 418
PctI GAATGC 1 cut(s) 82
PdmI GAANNNNTTC 2 cut(s) 427, 596
PfeI GAWTC 3 cut(s) 238, 597, 1985
PflFI GACNNNGTC 1 cut(s) 478
PkrI GCNGC 6 cut(s) 555, 610, 657, 1162, 1231, 1720
PleI GAGTC 6 cut(s) 502, 726, 1583, 1592, 1663, 1735
PpsI GAGTC 6 cut(s) 502, 726, 1583, 1592, 1663, 1735
PpuMI RGGWCCY 1 cut(s) 1611
PshBI ATTAAT 1 cut(s) 2075
PsiI TTATAA 1 cut(s) 1167
Psp5II RGGWCCY 1 cut(s) 1611
Psp6I CCWGG 5 cut(s) 205, 280, 708, 820, 955
PspGI CCWGG 5 cut(s) 205, 280, 708, 820, 955
PspN4I GGNNCC 6 cut(s) 917, 1613, 1713, 1833, 1960, 2008
PspOMI GGGCCC 1 cut(s) 915
PspPI GGNCC 4 cut(s) 915, 916, 1478, 1611
PspPPI RGGWCCY 1 cut(s) 1611
PstI CTGCAG 2 cut(s) 610, 1720
PstNI CAGNNNCTG 1 cut(s) 86
PsuI RGATCY 3 cut(s) 212, 1068, 1831
PsyI GACNNNGTC 1 cut(s) 478
RsaI GTAC 4 cut(s) 773, 1452, 1860, 1960
RsaNI GTAC 4 cut(s) 772, 1451, 1859, 1959
RseI CAYNNNNRTG 3 cut(s) 1257, 1853, 2052
SalI GTCGAC 1 cut(s) 1586
SapI GCTCTTC 1 cut(s) 418
SaqAI TTAA 7 cut(s) 869, 1056, 1104, 1607, 1619, 2075, 2107
SatI GCNGC 6 cut(s) 554, 609, 656, 1161, 1230, 1719
Sau96I GGNCC 4 cut(s) 915, 916, 1478, 1611
SchI GAGTC 6 cut(s) 503, 727, 1583, 1593, 1664, 1736
ScrFI CCNGG 7 cut(s) 207, 282, 710, 822, 919, 920, 957
SduI GDGCHC 5 cut(s) 31, 401, 887, 919, 925
SfaNI GCATC 2 cut(s) 454, 1713
SfcI CTRYAG 4 cut(s) 489, 511, 606, 1716
Sfr274I CTCGAG 2 cut(s) 246, 576
SfuI TTCGAA 1 cut(s) 371
SinI GGWCC 1 cut(s) 1611
SlaI CTCGAG 2 cut(s) 246, 576
SmaI CCCGGG 1 cut(s) 920
SmiMI CAYNNNNRTG 3 cut(s) 1257, 1853, 2052
SmlI CTYRAG 5 cut(s) 246, 576, 868, 995, 1606
SmoI CTYRAG 5 cut(s) 246, 576, 868, 995, 1606
SpeI ACTAGT 1 cut(s) 754
SrfI GCCCGGGC 1 cut(s) 920
SsiI CCGC 2 cut(s) 485, 1358
SspI AATATT 1 cut(s) 662
SspMI CTAG 4 cut(s) 102, 755, 912, 942
StyD4I CCNGG 7 cut(s) 205, 280, 708, 820, 917, 918, 955
StyI CCWWGG 3 cut(s) 101, 384, 612
TaaI ACNGT 9 cut(s) 480, 512, 741, 986, 1026, 1184, 1489, 1528, 1863
TaiI ACGT 1 cut(s) 1646
TaqI TCGA 7 cut(s) 247, 371, 577, 976, 1324, 1587, 1916
TatI WGTACW 1 cut(s) 771
TfiI GAWTC 3 cut(s) 238, 597, 1985
Tru1I TTAA 7 cut(s) 869, 1056, 1104, 1607, 1619, 2075, 2107
Tru9I TTAA 7 cut(s) 869, 1056, 1104, 1607, 1619, 2075, 2107
TscAI CASTG 5 cut(s) 31, 72, 483, 1797, 2008
TseFI GTSAC 3 cut(s) 691, 1026, 1433
TseI GCWGC 6 cut(s) 553, 608, 655, 1160, 1229, 1718
Tsp45I GTSAC 3 cut(s) 691, 1026, 1433
TspGWI ACGGA 1 cut(s) 174
TspMI CCCGGG 1 cut(s) 918
TspRI CASTG 5 cut(s) 31, 72, 483, 1797, 2008
Tth111I GACNNNGTC 1 cut(s) 478
Vha464I CTTAAG 2 cut(s) 868, 1606
VneI GTGCAC 1 cut(s) 27
VpaK11BI GGWCC 1 cut(s) 1611
VspI ATTAAT 1 cut(s) 2075
XagI CCTNNNNNAGG 1 cut(s) 861
XapI RAATTY 6 cut(s) 19, 51, 434, 874, 1555, 1942
XcmI CCANNNNNNNNNTGG 1 cut(s) 1852
XhoI CTCGAG 2 cut(s) 246, 576
XmaI CCCGGG 1 cut(s) 918
XmaJI CCTAGG 1 cut(s) 101
XmiI GTMKAC 2 cut(s) 1571, 1587
XmnI GAANNNNTTC 2 cut(s) 427, 596
XspI CTAG 4 cut(s) 102, 755, 912, 942
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.