pycom05g21180

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0013210
Physical Location & Seq
Reverse (-)
23872410 .. 23877049
4640 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g21180.11

Sequence Viewer

Length: 729 bp
ATGCACAAGTCGGGTAGAGGCCAACGTGAAAAGCTTCAGCAGTTCATCATGATAACTGGAGCGAGCGAAAAAGTGGCTTTGCAGGCTCTGAAGTCTAGTGATTGGCATCTTGAAGGAGCGTTTGATGTATTCTACAGTCAGCCCCAGCTGCAAACACATACTGATTCTAGGCATTTGGAGGAATTATACAATAGATATAAAGATCCACGTGTTGACATGATACTTGTTGATGGTATCAGCCTCCTTTGCAATGATCTTCAGGTGGATCCTCAAGATATTGTCATGTTAGTTGTTTCCTGGCACATGAAGGCAGCTACCATGTGTGAATTTTCCAAGCAGGAGTTCATAGATGGATTAGAATCACTAGGGATAGATTCTTTGGACAAGTTCCGTGAAAAGATACCATTTAAAAAGTTTCGAGAGATATATAATTTTGCTTTTGGCTGGGCAAAAGAAAAGGGTCAGAAATCTTTGGCATTGGATACTGCAATTGGAATGTGGCAATTACTGTTTGCTGAAAAGCAGTGGCCGTATGTTGAACACTGGTGTCAGTTCTTACAGGCTCGGCATAACAAGGCAATTTCAAGGGACACATGGTCTCAACTATTGGAGTTTGCGAGGGCTGTGGATTCATCACTGTCAAACTATGATGCGGAAGGTGCCTGGCCATATCTTATTGACGAATTTGTCGAGTACTTGAAGGAGAACGGCGTAATCCCAAAGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding UBA_4

Protein Analysis

243

Amino Acids

28.15

Weight (kDa)

5.54

Isoelectric Point (pI)

32.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013210)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12760
fragaria_vesca FvH4_3g14590 FvH4_3g14590
malus_domestica MD05G1234900.v1.1 MD10G1211100.v1.1
prunus_persica Prupe.4G131300_v2.0.a1
pyrus_communis pycom05g21180 pycom10g17940
rosa_chinensis RchiOBHm_Chr5g0024051
rosa_laevigata RLG00000032757
rosa_multiflora Rmu_sc0011761.1_g000007
rosa_roxburghii Rroxscaffold_1G00055190
rosa_rugosa Rorug05G0081200 Rorug05G0081200
rosa_samantha Rh5AG171600 Rh5CG183800 Rh5DG170400
rosa_wichuraiana Rw5G015530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 686
AccB1I GGYRCC 1 cut(s) 659
AciI CCGC 1 cut(s) 653
AclWI GGATC 3 cut(s) 197, 260, 273
AcoI YGGCCR 2 cut(s) 527, 665
AcsI RAATTY 2 cut(s) 326, 683
AcuI CTGAAG 3 cut(s) 20, 110, 242
AcvI CACGTG 1 cut(s) 209
AfaI GTAC 1 cut(s) 695
AflIII ACRYGT 1 cut(s) 208
AgsI TTSAA 4 cut(s) 113, 539, 585, 700
AhdI GACNNNNNGTC 1 cut(s) 595
AjnI CCWGG 2 cut(s) 296, 662
AjuI GAANNNNNNNTTGG 2 cut(s) 326, 358
AluBI AGCT 3 cut(s) 34, 148, 314
AluI AGCT 3 cut(s) 34, 148, 314
Alw26I GTCTC 1 cut(s) 603
AlwI GGATC 3 cut(s) 197, 260, 273
AlwNI CAGNNNCTG 1 cut(s) 88
AoxI GGCC 3 cut(s) 19, 527, 665
ApeKI GCWGC 2 cut(s) 148, 311
ApoI RAATTY 2 cut(s) 326, 683
Asp700I GAANNNNTTC 1 cut(s) 33
BalI TGGCCA 1 cut(s) 667
BamHI GGATCC 1 cut(s) 265
BanI GGYRCC 1 cut(s) 659
BbrPI CACGTG 1 cut(s) 209
BbvI GCAGC 2 cut(s) 135, 323
BccI CCATC 2 cut(s) 224, 344
BceAI ACGGC 2 cut(s) 514, 724
BciT130I CCWGG 2 cut(s) 298, 664
BciVI GTATCC 1 cut(s) 475
BcoDI GTCTC 1 cut(s) 603
BfaI CTAG 3 cut(s) 96, 168, 365
BfmI CTRYAG 1 cut(s) 133
BfuI GTATCC 1 cut(s) 475
BisI GCNGC 2 cut(s) 149, 312
BlsI GCNGC 2 cut(s) 150, 313
BmcAI AGTACT 1 cut(s) 695
Bme1390I CCNGG 2 cut(s) 298, 664
BmeRI GACNNNNNGTC 1 cut(s) 595
BmiI GGNNCC 2 cut(s) 267, 661
BmrFI CCNGG 2 cut(s) 298, 664
BmsI GCATC 2 cut(s) 115, 640
BpmI CTGGAG 1 cut(s) 78
BpuEI CTTGAG 1 cut(s) 255
BsaAI YACGTR 1 cut(s) 209
BsaBI GATNNNNATC 2 cut(s) 105, 358
BsaI GGTCTC 1 cut(s) 603
Bse1I ACTGG 2 cut(s) 61, 548
Bse3DI GCAATG 1 cut(s) 256
Bse8I GATNNNNATC 2 cut(s) 105, 358
BseBI CCWGG 2 cut(s) 298, 664
BseJI GATNNNNATC 2 cut(s) 105, 358
BseMI GCAATG 1 cut(s) 256
BseNI ACTGG 2 cut(s) 61, 548
BseXI GCAGC 2 cut(s) 135, 323
BseYI CCCAGC 2 cut(s) 144, 444
BshFI GGCC 3 cut(s) 21, 529, 667
BshNI GGYRCC 1 cut(s) 659
BslFI GGGAC 1 cut(s) 602
BsmAI GTCTC 1 cut(s) 603
BsmFI GGGAC 1 cut(s) 602
BsnI GGCC 3 cut(s) 21, 529, 667
Bso31I GGTCTC 1 cut(s) 603
Bsp143I GATC 3 cut(s) 202, 253, 265
BspACI CCGC 1 cut(s) 653
BspANI GGCC 3 cut(s) 21, 529, 667
BspHI TCATGA 1 cut(s) 48
BspLI GGNNCC 2 cut(s) 267, 661
BspPI GGATC 3 cut(s) 197, 260, 273
BspT107I GGYRCC 1 cut(s) 659
BspTNI GGTCTC 1 cut(s) 603
BsrDI GCAATG 1 cut(s) 256
BsrI ACTGG 2 cut(s) 61, 548
BssMI GATC 3 cut(s) 202, 253, 265
Bst2UI CCWGG 2 cut(s) 298, 664
Bst4CI ACNGT 3 cut(s) 137, 510, 639
BstBAI YACGTR 1 cut(s) 209
BstC8I GCNNGC 2 cut(s) 64, 84
BstKTI GATC 3 cut(s) 205, 256, 268
BstMAI GTCTC 1 cut(s) 603
BstMBI GATC 3 cut(s) 202, 253, 265
BstMWI GCNNNNNNNGC 4 cut(s) 83, 148, 246, 659
BstNI CCWGG 2 cut(s) 298, 664
BstSCI CCNGG 2 cut(s) 296, 662
BstSFI CTRYAG 1 cut(s) 133
BstV1I GCAGC 2 cut(s) 135, 323
BstX2I RGATCY 2 cut(s) 202, 265
BstYI RGATCY 2 cut(s) 202, 265
BsuI GTATCC 1 cut(s) 475
BsuRI GGCC 3 cut(s) 21, 529, 667
BtsI GCAGTG 1 cut(s) 530
BtsIMutI CAGTG 3 cut(s) 530, 541, 635
Cac8I GCNNGC 2 cut(s) 64, 84
CaiI CAGNNNCTG 1 cut(s) 88
CciI TCATGA 1 cut(s) 48
Csp6I GTAC 1 cut(s) 694
CviAII CATG 6 cut(s) 49, 217, 283, 304, 319, 594
CviQI GTAC 1 cut(s) 694
DpnI GATC 3 cut(s) 204, 255, 267
DpnII GATC 3 cut(s) 202, 253, 265
DraI TTTAAA 1 cut(s) 409
DrdI GACNNNNNNGTC 1 cut(s) 686
DriI GACNNNNNGTC 1 cut(s) 595
DseDI GACNNNNNNGTC 1 cut(s) 686
EaeI YGGCCR 2 cut(s) 527, 665
Eam1105I GACNNNNNGTC 1 cut(s) 595
Eco31I GGTCTC 1 cut(s) 603
Eco57I CTGAAG 3 cut(s) 20, 110, 242
Eco72I CACGTG 1 cut(s) 209
EcoRII CCWGG 2 cut(s) 296, 662
FaeI CATG 6 cut(s) 52, 220, 286, 307, 322, 597
FaqI GGGAC 1 cut(s) 602
FatI CATG 6 cut(s) 48, 216, 282, 303, 318, 593
Fnu4HI GCNGC 2 cut(s) 149, 312
Fsp4HI GCNGC 2 cut(s) 149, 312
FspBI CTAG 3 cut(s) 96, 168, 365
GluI GCNGC 2 cut(s) 149, 312
GsaI CCCAGC 2 cut(s) 148, 448
GsuI CTGGAG 1 cut(s) 78
HaeIII GGCC 3 cut(s) 21, 529, 667
Hin1II CATG 6 cut(s) 52, 220, 286, 307, 322, 597
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HindIII AAGCTT 1 cut(s) 32
HinfI GANTC 4 cut(s) 164, 359, 374, 629
Hpy166II GTNNAC 1 cut(s) 214
Hpy188I TCNGA 2 cut(s) 90, 465
Hpy188III TCNNGA 4 cut(s) 49, 110, 272, 419
Hpy8I GTNNAC 1 cut(s) 214
HpyAV CCTTC 4 cut(s) 107, 301, 650, 694
HpyCH4III ACNGT 3 cut(s) 137, 510, 639
HpyCH4IV ACGT 2 cut(s) 25, 208
HpyCH4V TGCA 5 cut(s) 4, 82, 151, 249, 488
HpyF10VI GCNNNNNNNGC 4 cut(s) 83, 148, 246, 659
HpySE526I ACGT 2 cut(s) 25, 208
Hsp92II CATG 6 cut(s) 52, 220, 286, 307, 322, 597
Kzo9I GATC 3 cut(s) 202, 253, 265
LmnI GCTCC 2 cut(s) 59, 116
Lsp1109I GCAGC 2 cut(s) 135, 323
LweI GCATC 2 cut(s) 115, 640
MaeI CTAG 3 cut(s) 96, 168, 365
MaeII ACGT 2 cut(s) 25, 208
MalI GATC 3 cut(s) 204, 255, 267
MboI GATC 3 cut(s) 202, 253, 265
MboII GAAGA 1 cut(s) 248
MfeI CAATTG 1 cut(s) 489
MflI RGATCY 2 cut(s) 202, 265
MlsI TGGCCA 1 cut(s) 667
MluCI AATT 7 cut(s) 182, 326, 430, 489, 503, 579, 683
MluNI TGGCCA 1 cut(s) 667
MnlI CCTC 5 cut(s) 11, 172, 251, 279, 612
Mox20I TGGCCA 1 cut(s) 667
MroXI GAANNNNTTC 1 cut(s) 33
MscI TGGCCA 1 cut(s) 667
MseI TTAA 2 cut(s) 408, 727
Msp20I TGGCCA 1 cut(s) 667
MspA1I CMGCKG 1 cut(s) 148
MspR9I CCNGG 2 cut(s) 298, 664
MunI CAATTG 1 cut(s) 489
MvaI CCWGG 2 cut(s) 298, 664
MwoI GCNNNNNNNGC 4 cut(s) 83, 148, 246, 659
NdeII GATC 3 cut(s) 202, 253, 265
NlaIII CATG 6 cut(s) 52, 220, 286, 307, 322, 597
NlaIV GGNNCC 2 cut(s) 267, 661
NmeAIII GCCGAG 1 cut(s) 544
PagI TCATGA 1 cut(s) 48
PcsI WCGNNNNNNNCGW 1 cut(s) 687
PdmI GAANNNNTTC 1 cut(s) 33
PfeI GAWTC 4 cut(s) 164, 359, 374, 629
PkrI GCNGC 2 cut(s) 150, 313
PmaCI CACGTG 1 cut(s) 209
PmlI CACGTG 1 cut(s) 209
Ppu21I YACGTR 1 cut(s) 209
Psp6I CCWGG 2 cut(s) 296, 662
PspCI CACGTG 1 cut(s) 209
PspFI CCCAGC 2 cut(s) 144, 444
PspGI CCWGG 2 cut(s) 296, 662
PspN4I GGNNCC 2 cut(s) 267, 661
PstNI CAGNNNCTG 1 cut(s) 88
PsuI RGATCY 2 cut(s) 202, 265
PvuII CAGCTG 1 cut(s) 148
RsaI GTAC 1 cut(s) 695
RsaNI GTAC 1 cut(s) 694
SaqAI TTAA 2 cut(s) 408, 727
SatI GCNGC 2 cut(s) 149, 312
Sau3AI GATC 3 cut(s) 202, 253, 265
ScaI AGTACT 1 cut(s) 695
ScrFI CCNGG 2 cut(s) 298, 664
SetI ASST 7 cut(s) 28, 36, 150, 211, 264, 316, 661
SfaNI GCATC 2 cut(s) 115, 640
SfcI CTRYAG 1 cut(s) 133
SmlI CTYRAG 1 cut(s) 270
SmoI CTYRAG 1 cut(s) 270
Sse9I AATT 7 cut(s) 182, 326, 430, 489, 503, 579, 683
SsiI CCGC 1 cut(s) 653
SspMI CTAG 3 cut(s) 96, 168, 365
StyD4I CCNGG 2 cut(s) 296, 662
TaaI ACNGT 3 cut(s) 137, 510, 639
TaiI ACGT 2 cut(s) 28, 211
TaqI TCGA 2 cut(s) 418, 690
TasI AATT 7 cut(s) 182, 326, 430, 489, 503, 579, 683
TatI WGTACW 1 cut(s) 693
TfiI GAWTC 4 cut(s) 164, 359, 374, 629
Tru1I TTAA 2 cut(s) 408, 727
Tru9I TTAA 2 cut(s) 408, 727
TscAI CASTG 3 cut(s) 530, 548, 642
TseI GCWGC 2 cut(s) 148, 311
TspDTI ATGAA 4 cut(s) 34, 320, 334, 621
TspGWI ACGGA 1 cut(s) 380
TspRI CASTG 3 cut(s) 530, 548, 642
XapI RAATTY 2 cut(s) 326, 683
XmnI GAANNNNTTC 1 cut(s) 33
XspI CTAG 3 cut(s) 96, 168, 365
ZrmI AGTACT 1 cut(s) 695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.