Rroxscaffold_1G00055190

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0013210
Physical Location & Seq
Reverse (-)
76674877 .. 76679857
4981 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00055190.1

Sequence Viewer

Length: 888 bp
ATGAGCAAGTTGGGTAGAGGCCAACGCGAGAAGATGCAACAGTTTATGACAATAACTGGAGCGAGCTTAAATGAGATACTCCTGGTGCCTTGCACTTATAAGGCAGGGCTTGGCAAAATAGAAAGTATCAGCAGATTGTGCTTGTTTGGTGTTAGTTTTGTTCTTGTTCCTGTACTAATATTTTCTGTAGCAGTTTTGTTGTTAAAAGATGATAAAGTCTCTCTGCAGACTCTGAAGGCGAGTGATTGGCATCTTGAAGGAGCATTTGATATATTCTACAGCCAGCCTCAGATTCAAGCACATACTGATTCTAGACATTTGGAGAAACTATACAATAGATATAAAGGCAAGTTATGTCCTGCCACTTGGTATCCAAGTGTTGACATGATACTCGCTGATGGTATCAGTCTCCTTTGCACTGATCTCAAGGTGGATCCTCAAGATATAGTCATGTTAGTTGTTTCCTGGCACATGAAGGCAGCCACCATGTGTGAATTTTCCAAGAATGAGTTCATAGATGGATTAGAAGCACTAGGGATAGATTCTCTGGAGAAGTTCCGCGAAAAGATACCCTTTATGCGATCTGAGCTAAGAGATGAACGTAGCACTTTGCGAGAGGGTCAGAAGTCACTGGCATTGGATACTGCAATTGGAATGTGGCAATTACTGTTTGCTGAAAAGCAGTGGCCGTATGTTGAGCATTGGTGTCAGTTCTTACAGGCTCGGCATAACAAGGCAATTTCTAGGGACACATGGTCCCAACTACTAGAGTTTGCAAGGACTGTTGATTCATCACTATCAAATTATGATGCGGAAGGTGCTTGGCCCTATCTCATAGACGAATTTGTGGAGTACTTGAAGGAGAATGGCGTAATCAAAAGTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding UBA_4

Protein Analysis

295

Amino Acids

33.76

Weight (kDa)

5.75

Isoelectric Point (pI)

34.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin_binding PF03556 205 - 285 1.6e-28 Cullin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013210)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12760
fragaria_vesca FvH4_3g14590 FvH4_3g14590
malus_domestica MD05G1234900.v1.1 MD10G1211100.v1.1
prunus_persica Prupe.4G131300_v2.0.a1
pyrus_communis pycom05g21180 pycom10g17940
rosa_chinensis RchiOBHm_Chr5g0024051
rosa_laevigata RLG00000032757
rosa_multiflora Rmu_sc0011761.1_g000007
rosa_roxburghii Rroxscaffold_1G00055190
rosa_rugosa Rorug05G0081200 Rorug05G0081200
rosa_samantha Rh5AG171600 Rh5CG183800 Rh5DG170400
rosa_wichuraiana Rw5G015530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 99
AccB1I GGYRCC 1 cut(s) 85
AccII CGCG 2 cut(s) 27, 561
AciI CCGC 2 cut(s) 559, 812
AclWI GGATC 2 cut(s) 428, 441
AcoI YGGCCR 1 cut(s) 686
AcsI RAATTY 2 cut(s) 494, 842
AcuI CTGAAG 1 cut(s) 254
AdeI CACNNNGTG 1 cut(s) 489
AfaI GTAC 2 cut(s) 174, 854
AgsI TTSAA 3 cut(s) 257, 296, 859
AhdI GACNNNNNGTC 1 cut(s) 754
AjnI CCWGG 2 cut(s) 81, 464
AjuI GAANNNNNNNTTGG 2 cut(s) 494, 526
AluBI AGCT 2 cut(s) 66, 589
AluI AGCT 2 cut(s) 66, 589
Alw26I GTCTC 2 cut(s) 223, 413
AlwI GGATC 2 cut(s) 428, 441
AlwNI CAGNNNCTG 1 cut(s) 232
AoxI GGCC 3 cut(s) 19, 686, 824
ApeKI GCWGC 1 cut(s) 479
ApoI RAATTY 2 cut(s) 494, 842
Asp700I GAANNNNTTC 1 cut(s) 509
AspS9I GGNCC 2 cut(s) 756, 825
AvaII GGWCC 1 cut(s) 756
BamHI GGATCC 1 cut(s) 433
BanI GGYRCC 1 cut(s) 85
BbvI GCAGC 1 cut(s) 491
BccI CCATC 2 cut(s) 392, 512
BceAI ACGGC 1 cut(s) 673
BciT130I CCWGG 2 cut(s) 83, 466
BciVI GTATCC 2 cut(s) 381, 634
BcoDI GTCTC 2 cut(s) 223, 413
BfaI CTAG 4 cut(s) 312, 533, 744, 767
BfmI CTRYAG 3 cut(s) 186, 224, 277
BfuI GTATCC 2 cut(s) 381, 634
BisI GCNGC 1 cut(s) 480
BlsI GCNGC 1 cut(s) 481
BmcAI AGTACT 1 cut(s) 854
Bme1390I CCNGG 2 cut(s) 83, 466
Bme18I GGWCC 1 cut(s) 756
BmeRI GACNNNNNGTC 1 cut(s) 754
BmgT120I GGNCC 2 cut(s) 756, 825
BmiI GGNNCC 3 cut(s) 87, 435, 758
BmrFI CCNGG 2 cut(s) 83, 466
BmsI GCATC 3 cut(s) 24, 259, 799
BpmI CTGGAG 2 cut(s) 78, 569
BpuEI CTTGAG 2 cut(s) 410, 423
BsaBI GATNNNNATC 1 cut(s) 249
Bse1I ACTGG 2 cut(s) 61, 636
Bse8I GATNNNNATC 1 cut(s) 249
BseBI CCWGG 2 cut(s) 83, 466
BseJI GATNNNNATC 1 cut(s) 249
BseMII CTCAG 2 cut(s) 302, 576
BseNI ACTGG 2 cut(s) 61, 636
BseXI GCAGC 1 cut(s) 491
Bsh1236I CGCG 2 cut(s) 27, 561
BshFI GGCC 3 cut(s) 21, 688, 826
BshNI GGYRCC 1 cut(s) 85
BslFI GGGAC 2 cut(s) 742, 761
BsmAI GTCTC 2 cut(s) 223, 413
BsmFI GGGAC 2 cut(s) 742, 761
BsnI GGCC 3 cut(s) 21, 688, 826
Bsp143I GATC 3 cut(s) 421, 433, 581
BspACI CCGC 2 cut(s) 559, 812
BspANI GGCC 3 cut(s) 21, 688, 826
BspCNI CTCAG 2 cut(s) 301, 577
BspFNI CGCG 2 cut(s) 27, 561
BspLI GGNNCC 3 cut(s) 87, 435, 758
BspMAI CTGCAG 1 cut(s) 228
BspPI GGATC 2 cut(s) 428, 441
BspT107I GGYRCC 1 cut(s) 85
BsrI ACTGG 2 cut(s) 61, 636
BssMI GATC 3 cut(s) 421, 433, 581
Bst2UI CCWGG 2 cut(s) 83, 466
Bst4CI ACNGT 3 cut(s) 42, 669, 784
BstAPI GCANNNNNTGC 1 cut(s) 138
BstC8I GCNNGC 2 cut(s) 64, 284
BstDEI CTNAG 3 cut(s) 288, 585, 590
BstFNI CGCG 2 cut(s) 27, 561
BstKTI GATC 3 cut(s) 424, 436, 584
BstMAI GTCTC 2 cut(s) 223, 413
BstMBI GATC 3 cut(s) 421, 433, 581
BstMWI GCNNNNNNNGC 3 cut(s) 138, 586, 818
BstNI CCWGG 2 cut(s) 83, 466
BstSCI CCNGG 2 cut(s) 81, 464
BstSFI CTRYAG 3 cut(s) 186, 224, 277
BstUI CGCG 2 cut(s) 27, 561
BstV1I GCAGC 1 cut(s) 491
BstX2I RGATCY 1 cut(s) 433
BstYI RGATCY 1 cut(s) 433
BsuI GTATCC 2 cut(s) 381, 634
BsuRI GGCC 3 cut(s) 21, 688, 826
BtsI GCAGTG 1 cut(s) 689
BtsIMutI CAGTG 3 cut(s) 417, 629, 689
Cac8I GCNNGC 2 cut(s) 64, 284
CaiI CAGNNNCTG 1 cut(s) 232
Cfr13I GGNCC 2 cut(s) 756, 825
Csp6I GTAC 2 cut(s) 173, 853
CviAII CATG 5 cut(s) 385, 451, 472, 487, 753
CviQI GTAC 2 cut(s) 173, 853
DdeI CTNAG 3 cut(s) 288, 585, 590
DpnI GATC 3 cut(s) 423, 435, 583
DpnII GATC 3 cut(s) 421, 433, 581
DraIII CACNNNGTG 1 cut(s) 489
DriI GACNNNNNGTC 1 cut(s) 754
EaeI YGGCCR 1 cut(s) 686
Eam1105I GACNNNNNGTC 1 cut(s) 754
Eco47I GGWCC 1 cut(s) 756
Eco57I CTGAAG 1 cut(s) 254
EcoRII CCWGG 2 cut(s) 81, 464
FaeI CATG 5 cut(s) 388, 454, 475, 490, 756
FalI AAGNNNNNCTT 2 cut(s) 557, 589
FaqI GGGAC 2 cut(s) 742, 761
FatI CATG 5 cut(s) 384, 450, 471, 486, 752
Fnu4HI GCNGC 1 cut(s) 480
Fsp4HI GCNGC 1 cut(s) 480
FspBI CTAG 4 cut(s) 312, 533, 744, 767
GluI GCNGC 1 cut(s) 480
GsuI CTGGAG 2 cut(s) 78, 569
HaeIII GGCC 3 cut(s) 21, 688, 826
Hin1II CATG 5 cut(s) 388, 454, 475, 490, 756
HincII GTYRAC 1 cut(s) 382
HindII GTYRAC 1 cut(s) 382
HinfI GANTC 5 cut(s) 229, 292, 308, 542, 788
Hpy166II GTNNAC 1 cut(s) 382
Hpy188I TCNGA 4 cut(s) 234, 291, 586, 624
Hpy188III TCNNGA 4 cut(s) 254, 312, 440, 548
Hpy8I GTNNAC 1 cut(s) 382
HpyAV CCTTC 5 cut(s) 229, 251, 469, 809, 853
HpyCH4III ACNGT 3 cut(s) 42, 669, 784
HpyCH4IV ACGT 1 cut(s) 601
HpyCH4V TGCA 6 cut(s) 37, 93, 226, 417, 647, 776
HpyF10VI GCNNNNNNNGC 3 cut(s) 138, 586, 818
HpyF3I CTNAG 3 cut(s) 288, 585, 590
HpySE526I ACGT 1 cut(s) 601
Hsp92II CATG 5 cut(s) 388, 454, 475, 490, 756
Kzo9I GATC 3 cut(s) 421, 433, 581
LmnI GCTCC 2 cut(s) 59, 260
Lsp1109I GCAGC 1 cut(s) 491
LweI GCATC 3 cut(s) 24, 259, 799
MaeI CTAG 4 cut(s) 312, 533, 744, 767
MaeII ACGT 1 cut(s) 601
MaeIII GTNAC 1 cut(s) 627
MalI GATC 3 cut(s) 423, 435, 583
MboI GATC 3 cut(s) 421, 433, 581
MboII GAAGA 1 cut(s) 43
MfeI CAATTG 1 cut(s) 648
MflI RGATCY 1 cut(s) 433
MluCI AATT 6 cut(s) 494, 648, 662, 738, 802, 842
MlyI GAGTC 1 cut(s) 223
MnlI CCTC 4 cut(s) 11, 297, 447, 610
MroXI GAANNNNTTC 1 cut(s) 509
MseI TTAA 2 cut(s) 68, 203
MspR9I CCNGG 2 cut(s) 83, 466
MunI CAATTG 1 cut(s) 648
MvaI CCWGG 2 cut(s) 83, 466
MvnI CGCG 2 cut(s) 27, 561
MwoI GCNNNNNNNGC 3 cut(s) 138, 586, 818
NdeII GATC 3 cut(s) 421, 433, 581
NlaIII CATG 5 cut(s) 388, 454, 475, 490, 756
NlaIV GGNNCC 3 cut(s) 87, 435, 758
NmeAIII GCCGAG 1 cut(s) 703
NmuCI GTSAC 1 cut(s) 627
PdmI GAANNNNTTC 1 cut(s) 509
PfeI GAWTC 4 cut(s) 292, 308, 542, 788
PkrI GCNGC 1 cut(s) 481
PleI GAGTC 1 cut(s) 223
PpsI GAGTC 1 cut(s) 223
PsiI TTATAA 1 cut(s) 99
Psp6I CCWGG 2 cut(s) 81, 464
PspGI CCWGG 2 cut(s) 81, 464
PspN4I GGNNCC 3 cut(s) 87, 435, 758
PspPI GGNCC 2 cut(s) 756, 825
PstI CTGCAG 1 cut(s) 228
PstNI CAGNNNCTG 1 cut(s) 232
PsuI RGATCY 1 cut(s) 433
RsaI GTAC 2 cut(s) 174, 854
RsaNI GTAC 2 cut(s) 173, 853
SaqAI TTAA 2 cut(s) 68, 203
SatI GCNGC 1 cut(s) 480
Sau3AI GATC 3 cut(s) 421, 433, 581
Sau96I GGNCC 2 cut(s) 756, 825
ScaI AGTACT 1 cut(s) 854
SchI GAGTC 1 cut(s) 223
ScrFI CCNGG 2 cut(s) 83, 466
SetI ASST 5 cut(s) 68, 432, 591, 604, 820
SfaNI GCATC 3 cut(s) 24, 259, 799
SfcI CTRYAG 3 cut(s) 186, 224, 277
SinI GGWCC 1 cut(s) 756
SmlI CTYRAG 2 cut(s) 425, 438
SmoI CTYRAG 2 cut(s) 425, 438
Sse9I AATT 6 cut(s) 494, 648, 662, 738, 802, 842
SsiI CCGC 2 cut(s) 559, 812
SspI AATATT 1 cut(s) 180
SspMI CTAG 4 cut(s) 312, 533, 744, 767
StyD4I CCNGG 2 cut(s) 81, 464
TaaI ACNGT 3 cut(s) 42, 669, 784
TaiI ACGT 1 cut(s) 604
TasI AATT 6 cut(s) 494, 648, 662, 738, 802, 842
TatI WGTACW 2 cut(s) 172, 852
TfiI GAWTC 4 cut(s) 292, 308, 542, 788
Tru1I TTAA 2 cut(s) 68, 203
Tru9I TTAA 2 cut(s) 68, 203
TscAI CASTG 3 cut(s) 424, 636, 689
TseFI GTSAC 1 cut(s) 627
TseI GCWGC 1 cut(s) 479
Tsp45I GTSAC 1 cut(s) 627
TspDTI ATGAA 4 cut(s) 488, 502, 612, 780
TspRI CASTG 3 cut(s) 424, 636, 689
VpaK11BI GGWCC 1 cut(s) 756
XapI RAATTY 2 cut(s) 494, 842
XbaI TCTAGA 1 cut(s) 311
XmnI GAANNNNTTC 1 cut(s) 509
XspI CTAG 4 cut(s) 312, 533, 744, 767
ZrmI AGTACT 1 cut(s) 854
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.