pycom10g17940

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0013210
Physical Location & Seq
Reverse (-)
21085980 .. 21090499
4520 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g17940.4

Sequence Viewer

Length: 777 bp
ATGAACAAGTTGGGTAAAGGCCAACGCGATAAGCTCCAGCAGTTCGTCATGATAACTGGAGCGAGTGAAAAAGTGGCTCTGCAGGTTCTGAAGGAGAGTGATTGGCATCTTGAAGGAGCGTTTGATATCTTCTACACTCAGCCCCAGCTGCAAGCAGAGACTGATTCTAATACTGATTCTAATACCAATTCTAGACAACTGGAGGAATTATACAATACATATAAAGATCCAGATTCTGATATGATACTTGTTGATGGTATCAGCCTCCTTCTCAACGATCTTCAGGTGGATCCTCAAGATATAGTCATGTTAGTTGTTTCCTGGCACATGAAGGCAGCTACCATGTGTGAATTTTCCAAGGAGGAGTTCGTAGAGGGATTAGAACCACTAGGGATAGATTCTTTGGACAAGTTCCGTGAAAAGATACCATTTATGAGATCCGAGCTGAAAGATGAACAAAAGTTTCGAGAGATATATAATTTTGCTTTTGACTGGGCAAAGGAAAAGGGTCAGAAATCTTTGGCATTGGATACTGCTATTGGAATGTGGCAATTGGTGTTTGCTGAAAAGCAGTGGCCGTATGTAGAACATTGGTGTCAGTTCTTACCGGCTCGGCATAACAAGGCCATTTCGAGGGACACATGGTCTCAACTATTGGAGTTTGCAACGGCCGTGGATCCGTCACTGTCAAACTATGACGCGGAAGGTGCCTGGCCATATCTTATTGACGAATTTGTCGAGTACTTGAAGGAGAACGGAGCAATCCAAAAGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding UBA_4

Protein Analysis

259

Amino Acids

29.86

Weight (kDa)

4.6

Isoelectric Point (pI)

29.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013210)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12760
fragaria_vesca FvH4_3g14590 FvH4_3g14590
malus_domestica MD05G1234900.v1.1 MD10G1211100.v1.1
prunus_persica Prupe.4G131300_v2.0.a1
pyrus_communis pycom05g21180 pycom10g17940
rosa_chinensis RchiOBHm_Chr5g0024051
rosa_laevigata RLG00000032757
rosa_multiflora Rmu_sc0011761.1_g000007
rosa_roxburghii Rroxscaffold_1G00055190
rosa_rugosa Rorug05G0081200 Rorug05G0081200
rosa_samantha Rh5AG171600 Rh5CG183800 Rh5DG170400
rosa_wichuraiana Rw5G015530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 734
Acc36I ACCTGC 1 cut(s) 73
AccB1I GGYRCC 1 cut(s) 707
AccII CGCG 2 cut(s) 27, 701
AciI CCGC 1 cut(s) 701
AclWI GGATC 6 cut(s) 221, 284, 297, 432, 671, 684
AcoI YGGCCR 3 cut(s) 575, 669, 713
AcsI RAATTY 2 cut(s) 350, 731
AcuI CTGAAG 2 cut(s) 110, 266
AfaI GTAC 1 cut(s) 743
AfiI CCNNNNNNNGG 1 cut(s) 633
AgsI TTSAA 2 cut(s) 113, 748
AhdI GACNNNNNGTC 1 cut(s) 643
AjnI CCWGG 2 cut(s) 320, 710
AjuI GAANNNNNNNTTGG 2 cut(s) 350, 382
AluBI AGCT 4 cut(s) 34, 148, 338, 445
AluI AGCT 4 cut(s) 34, 148, 338, 445
Alw26I GTCTC 2 cut(s) 152, 651
AlwI GGATC 6 cut(s) 221, 284, 297, 432, 671, 684
AlwNI CAGNNNCTG 3 cut(s) 88, 161, 236
AoxI GGCC 5 cut(s) 19, 575, 624, 669, 713
ApeKI GCWGC 2 cut(s) 148, 335
ApoI RAATTY 2 cut(s) 350, 731
BalI TGGCCA 1 cut(s) 715
BamHI GGATCC 2 cut(s) 289, 676
BanI GGYRCC 1 cut(s) 707
BbvI GCAGC 2 cut(s) 135, 347
BccI CCATC 1 cut(s) 248
BceAI ACGGC 3 cut(s) 562, 656, 684
BciT130I CCWGG 2 cut(s) 322, 712
BciVI GTATCC 1 cut(s) 523
BcoDI GTCTC 2 cut(s) 152, 651
BfaI CTAG 2 cut(s) 192, 389
BfmI CTRYAG 1 cut(s) 80
BfuAI ACCTGC 1 cut(s) 73
BfuI GTATCC 1 cut(s) 523
BisI GCNGC 2 cut(s) 149, 336
BlsI GCNGC 2 cut(s) 150, 337
BmcAI AGTACT 1 cut(s) 743
Bme1390I CCNGG 2 cut(s) 322, 712
BmeRI GACNNNNNGTC 1 cut(s) 643
BmiI GGNNCC 3 cut(s) 291, 678, 709
BmrFI CCNGG 2 cut(s) 322, 712
BmrI ACTGGG 1 cut(s) 502
BmsI GCATC 1 cut(s) 115
BmuI ACTGGG 1 cut(s) 502
BpmI CTGGAG 3 cut(s) 20, 78, 221
BpuEI CTTGAG 1 cut(s) 279
BsaBI GATNNNNATC 1 cut(s) 105
BsaI GGTCTC 1 cut(s) 651
BsaJI CCNNGG 2 cut(s) 357, 672
Bsc4I CCNNNNNNNGG 1 cut(s) 633
Bse118I RCCGGY 1 cut(s) 607
Bse1I ACTGG 3 cut(s) 61, 204, 497
Bse8I GATNNNNATC 1 cut(s) 105
BseBI CCWGG 2 cut(s) 322, 712
BseDI CCNNGG 2 cut(s) 357, 672
BseJI GATNNNNATC 1 cut(s) 105
BseLI CCNNNNNNNGG 1 cut(s) 633
BseMII CTCAG 1 cut(s) 152
BseNI ACTGG 3 cut(s) 61, 204, 497
BseRI GAGGAG 1 cut(s) 377
BseX3I CGGCCG 1 cut(s) 669
BseXI GCAGC 2 cut(s) 135, 347
BseYI CCCAGC 1 cut(s) 144
Bsh1236I CGCG 2 cut(s) 27, 701
Bsh1285I CGRYCG 1 cut(s) 672
BshFI GGCC 5 cut(s) 21, 577, 626, 671, 715
BshNI GGYRCC 1 cut(s) 707
BsiEI CGRYCG 1 cut(s) 672
BsiSI CCGG 1 cut(s) 608
BslFI GGGAC 1 cut(s) 650
BslI CCNNNNNNNGG 1 cut(s) 633
BsmAI GTCTC 2 cut(s) 152, 651
BsmFI GGGAC 1 cut(s) 650
BsnI GGCC 5 cut(s) 21, 577, 626, 671, 715
Bso31I GGTCTC 1 cut(s) 651
Bsp143I GATC 5 cut(s) 226, 277, 289, 437, 676
BspACI CCGC 1 cut(s) 701
BspANI GGCC 5 cut(s) 21, 577, 626, 671, 715
BspCNI CTCAG 1 cut(s) 151
BspFNI CGCG 2 cut(s) 27, 701
BspHI TCATGA 1 cut(s) 48
BspLI GGNNCC 3 cut(s) 291, 678, 709
BspMAI CTGCAG 1 cut(s) 84
BspMI ACCTGC 1 cut(s) 73
BspPI GGATC 6 cut(s) 221, 284, 297, 432, 671, 684
BspT107I GGYRCC 1 cut(s) 707
BspTNI GGTCTC 1 cut(s) 651
BsrFI RCCGGY 1 cut(s) 607
BsrI ACTGG 3 cut(s) 61, 204, 497
BssAI RCCGGY 1 cut(s) 607
BssECI CCNNGG 2 cut(s) 357, 672
BssMI GATC 5 cut(s) 226, 277, 289, 437, 676
BssT1I CCWWGG 1 cut(s) 357
Bst2UI CCWGG 2 cut(s) 322, 712
Bst4CI ACNGT 1 cut(s) 687
BstC8I GCNNGC 1 cut(s) 153
BstDEI CTNAG 1 cut(s) 138
BstDSI CCRYGG 1 cut(s) 672
BstFNI CGCG 2 cut(s) 27, 701
BstKTI GATC 5 cut(s) 229, 280, 292, 440, 679
BstMAI GTCTC 2 cut(s) 152, 651
BstMBI GATC 5 cut(s) 226, 277, 289, 437, 676
BstMCI CGRYCG 1 cut(s) 672
BstMWI GCNNNNNNNGC 2 cut(s) 148, 707
BstNI CCWGG 2 cut(s) 322, 712
BstSCI CCNGG 2 cut(s) 320, 710
BstSFI CTRYAG 1 cut(s) 80
BstUI CGCG 2 cut(s) 27, 701
BstV1I GCAGC 2 cut(s) 135, 347
BstX2I RGATCY 4 cut(s) 226, 289, 437, 676
BstYI RGATCY 4 cut(s) 226, 289, 437, 676
BstZI CGGCCG 1 cut(s) 669
BsuI GTATCC 1 cut(s) 523
BsuRI GGCC 5 cut(s) 21, 577, 626, 671, 715
BtgI CCRYGG 1 cut(s) 672
BtsI GCAGTG 1 cut(s) 578
BtsIMutI CAGTG 2 cut(s) 578, 683
BveI ACCTGC 1 cut(s) 73
Cac8I GCNNGC 1 cut(s) 153
CaiI CAGNNNCTG 3 cut(s) 88, 161, 236
CciI TCATGA 1 cut(s) 48
Cfr10I RCCGGY 1 cut(s) 607
CseI GACGC 1 cut(s) 707
Csp6I GTAC 1 cut(s) 742
CspCI CAANNNNNGTGG 2 cut(s) 654, 689
CviAII CATG 5 cut(s) 49, 307, 328, 343, 642
CviQI GTAC 1 cut(s) 742
DdeI CTNAG 1 cut(s) 138
DpnI GATC 5 cut(s) 228, 279, 291, 439, 678
DpnII GATC 5 cut(s) 226, 277, 289, 437, 676
DrdI GACNNNNNNGTC 1 cut(s) 734
DriI GACNNNNNGTC 1 cut(s) 643
DseDI GACNNNNNNGTC 1 cut(s) 734
EaeI YGGCCR 3 cut(s) 575, 669, 713
EagI CGGCCG 1 cut(s) 669
Eam1105I GACNNNNNGTC 1 cut(s) 643
EclXI CGGCCG 1 cut(s) 669
Eco130I CCWWGG 1 cut(s) 357
Eco31I GGTCTC 1 cut(s) 651
Eco32I GATATC 1 cut(s) 127
Eco52I CGGCCG 1 cut(s) 669
Eco57I CTGAAG 2 cut(s) 110, 266
EcoRII CCWGG 2 cut(s) 320, 710
EcoRV GATATC 1 cut(s) 127
EcoT14I CCWWGG 1 cut(s) 357
ErhI CCWWGG 1 cut(s) 357
FaeI CATG 5 cut(s) 52, 310, 331, 346, 645
FaqI GGGAC 1 cut(s) 650
FatI CATG 5 cut(s) 48, 306, 327, 342, 641
Fnu4HI GCNGC 2 cut(s) 149, 336
Fsp4HI GCNGC 2 cut(s) 149, 336
FspBI CTAG 2 cut(s) 192, 389
GluI GCNGC 2 cut(s) 149, 336
GsaI CCCAGC 1 cut(s) 148
GsuI CTGGAG 3 cut(s) 20, 78, 221
HaeIII GGCC 5 cut(s) 21, 577, 626, 671, 715
HapII CCGG 1 cut(s) 608
HgaI GACGC 1 cut(s) 707
Hin1II CATG 5 cut(s) 52, 310, 331, 346, 645
HinfI GANTC 4 cut(s) 164, 176, 233, 398
HpaII CCGG 1 cut(s) 608
Hpy188I TCNGA 4 cut(s) 90, 238, 442, 513
Hpy188III TCNNGA 6 cut(s) 49, 110, 192, 230, 296, 467
HpyAV CCTTC 6 cut(s) 85, 107, 278, 325, 698, 742
HpyCH4III ACNGT 1 cut(s) 687
HpyCH4V TGCA 3 cut(s) 82, 151, 665
HpyF10VI GCNNNNNNNGC 2 cut(s) 148, 707
HpyF3I CTNAG 1 cut(s) 138
Hsp92II CATG 5 cut(s) 52, 310, 331, 346, 645
Kzo9I GATC 5 cut(s) 226, 277, 289, 437, 676
LmnI GCTCC 4 cut(s) 39, 59, 116, 758
Lsp1109I GCAGC 2 cut(s) 135, 347
LweI GCATC 1 cut(s) 115
MaeI CTAG 2 cut(s) 192, 389
MaeIII GTNAC 1 cut(s) 681
MalI GATC 5 cut(s) 228, 279, 291, 439, 678
MboI GATC 5 cut(s) 226, 277, 289, 437, 676
MboII GAAGA 2 cut(s) 121, 272
MfeI CAATTG 1 cut(s) 551
MflI RGATCY 4 cut(s) 226, 289, 437, 676
MlsI TGGCCA 1 cut(s) 715
MluCI AATT 6 cut(s) 187, 206, 350, 478, 551, 731
MluNI TGGCCA 1 cut(s) 715
MnlI CCTC 6 cut(s) 196, 275, 303, 355, 367, 627
Mox20I TGGCCA 1 cut(s) 715
MscI TGGCCA 1 cut(s) 715
MseI TTAA 1 cut(s) 775
Msp20I TGGCCA 1 cut(s) 715
MspA1I CMGCKG 1 cut(s) 148
MspI CCGG 1 cut(s) 608
MspR9I CCNGG 2 cut(s) 322, 712
MunI CAATTG 1 cut(s) 551
MvaI CCWGG 2 cut(s) 322, 712
MvnI CGCG 2 cut(s) 27, 701
MwoI GCNNNNNNNGC 2 cut(s) 148, 707
NdeII GATC 5 cut(s) 226, 277, 289, 437, 676
NlaIII CATG 5 cut(s) 52, 310, 331, 346, 645
NlaIV GGNNCC 3 cut(s) 291, 678, 709
NmeAIII GCCGAG 1 cut(s) 592
NmuCI GTSAC 1 cut(s) 681
PagI TCATGA 1 cut(s) 48
PcsI WCGNNNNNNNCGW 1 cut(s) 735
PfeI GAWTC 4 cut(s) 164, 176, 233, 398
PkrI GCNGC 2 cut(s) 150, 337
Psp6I CCWGG 2 cut(s) 320, 710
PspFI CCCAGC 1 cut(s) 144
PspGI CCWGG 2 cut(s) 320, 710
PspN4I GGNNCC 3 cut(s) 291, 678, 709
PstI CTGCAG 1 cut(s) 84
PstNI CAGNNNCTG 3 cut(s) 88, 161, 236
PsuI RGATCY 4 cut(s) 226, 289, 437, 676
PvuII CAGCTG 1 cut(s) 148
RsaI GTAC 1 cut(s) 743
RsaNI GTAC 1 cut(s) 742
SaqAI TTAA 1 cut(s) 775
SatI GCNGC 2 cut(s) 149, 336
Sau3AI GATC 5 cut(s) 226, 277, 289, 437, 676
ScaI AGTACT 1 cut(s) 743
ScrFI CCNGG 2 cut(s) 322, 712
SetI ASST 7 cut(s) 36, 87, 150, 288, 340, 447, 709
SfaNI GCATC 1 cut(s) 115
SfcI CTRYAG 1 cut(s) 80
SmlI CTYRAG 1 cut(s) 294
SmoI CTYRAG 1 cut(s) 294
Sse9I AATT 6 cut(s) 187, 206, 350, 478, 551, 731
SsiI CCGC 1 cut(s) 701
SspMI CTAG 2 cut(s) 192, 389
StyD4I CCNGG 2 cut(s) 320, 710
StyI CCWWGG 1 cut(s) 357
TaaI ACNGT 1 cut(s) 687
TaqI TCGA 3 cut(s) 466, 632, 738
TasI AATT 6 cut(s) 187, 206, 350, 478, 551, 731
TatI WGTACW 1 cut(s) 741
TfiI GAWTC 4 cut(s) 164, 176, 233, 398
Tru1I TTAA 1 cut(s) 775
Tru9I TTAA 1 cut(s) 775
TscAI CASTG 2 cut(s) 578, 690
TseFI GTSAC 1 cut(s) 681
TseI GCWGC 2 cut(s) 148, 335
Tsp45I GTSAC 1 cut(s) 681
TspDTI ATGAA 3 cut(s) 17, 344, 468
TspGWI ACGGA 3 cut(s) 404, 669, 771
TspRI CASTG 2 cut(s) 578, 690
XapI RAATTY 2 cut(s) 350, 731
XbaI TCTAGA 1 cut(s) 191
XspI CTAG 2 cut(s) 192, 389
ZrmI AGTACT 1 cut(s) 743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.