Rmu_sc0011761.1_g000007

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0011761.1
OG0013210
Physical Location & Seq
Forward (+)
34131 .. 41574
7444 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0011761.1_g000007.1.cds

Sequence Viewer

Length: 1245 bp
atgcaatcgacgtcgtcttccgtccatgaaggatccggtgagtggtggatctctctccgattcctcgccgtgccggactcgataacgacagcctgcagagccaccgacgatcgagtagtcaccggagtctccatcttggagggagccaggctcggaggcttcgggttcggaatccgagggaagagagagagagagagaatcagaaagctgtgcgcatcatggagacagagaggagagcgtcagagagactcatcaaaccctaacatcctctctccacaacctgttcgaggtttaagattcggctatcttcccacctccacaatgagcaagttgggtagaggccaacgcgagaagatgcaacagtttatgacaataactggagcgagtgataaagtctctctgcagactctgaaggcgagtgattggcatcttgaaggagcatttgatatattctacagccagcctcagattcaagcacatactgattctagacatttggagaaactatacaatagatataaaggcaagttatgtcctgccacttggtatccaagtgttgacatgatactcgctgatggtatcagtctcctttgcactgatctcaaggtggatcctcaagatatagtcatgttagttgtttcctggcacatgaaggcagccacgatgtgtgaattttccaagaatgagttcatagatggattagaagcactagggatagattctctggagaagttccgcgaaaagatgccctttatgcgatctgagctaagagatgaacagaagtttcgggagatatataattttgcttttggctgggcaaaagaaaagggtcagaagtcactggcattggatactgcaattggaatgtggcaattactgtttgctgaaaagcagtggccatatgttgagcattggtgtcagttcttacaggctcggcataacaaggcaatttctagggacacatggtcccaactactagagtttgcaagggctgttgattcatcactatcaaattatgatgcggaaggtgcttggccctatctcatagacgaatttgtcgagtacttgaaggagaatggcaacaaagccggaatccatcctggcgcttggagcttcggaggtctgatgacgaccctggcgtggcggcggagcttcggaggtgttcttcacgtggcaacggtgctcggaattggaggtgcggtcgatgttgacggcgacggagtgaggaagataacaccggtgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding UBA_4

Protein Analysis

414

Amino Acids

46.85

Weight (kDa)

8.41

Isoelectric Point (pI)

35.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013210)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12760
fragaria_vesca FvH4_3g14590 FvH4_3g14590
malus_domestica MD05G1234900.v1.1 MD10G1211100.v1.1
prunus_persica Prupe.4G131300_v2.0.a1
pyrus_communis pycom05g21180 pycom10g17940
rosa_chinensis RchiOBHm_Chr5g0024051
rosa_laevigata RLG00000032757
rosa_multiflora Rmu_sc0011761.1_g000007
rosa_roxburghii Rroxscaffold_1G00055190
rosa_rugosa Rorug05G0081200 Rorug05G0081200
rosa_samantha Rh5AG171600 Rh5CG183800 Rh5DG170400
rosa_wichuraiana Rw5G015530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1055
AatII GACGTC 1 cut(s) 14
Acc16I TGCGCA 1 cut(s) 214
AccII CGCG 2 cut(s) 348, 738
AciI CCGC 5 cut(s) 736, 1022, 1144, 1147, 1199
AclWI GGATC 5 cut(s) 27, 40, 56, 605, 618
AcoI YGGCCR 1 cut(s) 896
AcsI RAATTY 2 cut(s) 671, 1052
AcuI CTGAAG 1 cut(s) 431
AcvI CACGTG 1 cut(s) 1171
AcyI GRCGYC 1 cut(s) 11
AdeI CACNNNGTG 1 cut(s) 666
AfaI GTAC 1 cut(s) 1064
AfiI CCNNNNNNNGG 3 cut(s) 42, 287, 1140
AgeI ACCGGT 1 cut(s) 1237
AgsI TTSAA 3 cut(s) 434, 473, 1069
AhdI GACNNNNNGTC 1 cut(s) 964
AjnI CCWGG 4 cut(s) 146, 641, 1099, 1134
AjuI GAANNNNNNNTTGG 2 cut(s) 671, 703
AleI CACNNNNGTG 1 cut(s) 1240
AluBI AGCT 4 cut(s) 208, 766, 1113, 1152
AluI AGCT 4 cut(s) 208, 766, 1113, 1152
Alw21I GWGCWC 1 cut(s) 1185
Alw26I GTCTC 5 cut(s) 133, 217, 240, 400, 590
AlwI GGATC 5 cut(s) 27, 40, 56, 605, 618
AlwNI CAGNNNCTG 1 cut(s) 409
AoxI GGCC 3 cut(s) 340, 896, 1034
ApeKI GCWGC 1 cut(s) 656
ApoI RAATTY 2 cut(s) 671, 1052
AsiGI ACCGGT 1 cut(s) 1237
Asp700I GAANNNNTTC 1 cut(s) 686
AspLEI GCGC 2 cut(s) 215, 1106
AspS9I GGNCC 2 cut(s) 966, 1035
AsuHPI GGTGA 2 cut(s) 50, 112
AvaII GGWCC 1 cut(s) 966
BalI TGGCCA 1 cut(s) 898
BamHI GGATCC 2 cut(s) 32, 610
BbrPI CACGTG 1 cut(s) 1171
BbsI GAAGAC 1 cut(s) 9
Bbv12I GWGCWC 1 cut(s) 1185
BbvI GCAGC 1 cut(s) 668
BccI CCATC 4 cut(s) 140, 569, 689, 1104
BceAI ACGGC 2 cut(s) 53, 1228
BciT130I CCWGG 4 cut(s) 148, 643, 1101, 1136
BciVI GTATCC 2 cut(s) 558, 844
BcoDI GTCTC 5 cut(s) 133, 217, 240, 400, 590
BfaI CTAG 4 cut(s) 489, 710, 954, 977
BfmI CTRYAG 3 cut(s) 94, 401, 454
BfoI RGCGCY 1 cut(s) 1107
BfuI GTATCC 2 cut(s) 558, 844
BisI GCNGC 2 cut(s) 657, 1145
BlsI GCNGC 2 cut(s) 658, 1146
BmcAI AGTACT 1 cut(s) 1064
Bme1390I CCNGG 4 cut(s) 148, 643, 1101, 1136
Bme18I GGWCC 1 cut(s) 966
BmeRI GACNNNNNGTC 1 cut(s) 964
BmgT120I GGNCC 2 cut(s) 966, 1035
BmiI GGNNCC 4 cut(s) 34, 145, 612, 968
BmrFI CCNGG 4 cut(s) 148, 643, 1101, 1136
BmsI GCATC 5 cut(s) 224, 345, 436, 735, 1009
BpiI GAAGAC 1 cut(s) 9
BplI GAGNNNNNCTC 2 cut(s) 135, 167
BpmI CTGGAG 2 cut(s) 399, 746
BpuEI CTTGAG 2 cut(s) 587, 600
BsaAI YACGTR 1 cut(s) 1171
BsaBI GATNNNNATC 1 cut(s) 426
BsaHI GRCGYC 1 cut(s) 11
BsaJI CCNNGG 2 cut(s) 175, 1134
BsaWI WCCGGW 3 cut(s) 35, 122, 1237
BsaXI ACNNNNNCTCC 2 cut(s) 1185, 1215
Bsc4I CCNNNNNNNGG 3 cut(s) 42, 287, 1140
Bse118I RCCGGY 1 cut(s) 1237
Bse1I ACTGG 2 cut(s) 382, 846
Bse8I GATNNNNATC 1 cut(s) 426
BseBI CCWGG 4 cut(s) 148, 643, 1101, 1136
BseDI CCNNGG 2 cut(s) 175, 1134
BseGI GGATG 2 cut(s) 264, 1096
BseJI GATNNNNATC 1 cut(s) 426
BseLI CCNNNNNNNGG 3 cut(s) 42, 287, 1140
BseMII CTCAG 2 cut(s) 479, 753
BseNI ACTGG 2 cut(s) 382, 846
BseRI GAGGAG 1 cut(s) 246
BseXI GCAGC 1 cut(s) 668
BseYI CCCAGC 1 cut(s) 813
Bsh1236I CGCG 2 cut(s) 348, 738
Bsh1285I CGRYCG 2 cut(s) 112, 1203
BshFI GGCC 3 cut(s) 342, 898, 1036
BshTI ACCGGT 1 cut(s) 1237
BsiEI CGRYCG 2 cut(s) 112, 1203
BsiHKAI GWGCWC 1 cut(s) 1185
BsiSI CCGG 5 cut(s) 36, 74, 123, 1089, 1238
BslFI GGGAC 2 cut(s) 952, 971
BslI CCNNNNNNNGG 3 cut(s) 42, 287, 1140
BsmAI GTCTC 5 cut(s) 133, 217, 240, 400, 590
BsmFI GGGAC 2 cut(s) 952, 971
BsnI GGCC 3 cut(s) 342, 898, 1036
Bsp1286I GDGCHC 1 cut(s) 1185
Bsp143I GATC 6 cut(s) 32, 48, 109, 598, 610, 758
BspACI CCGC 5 cut(s) 736, 1022, 1144, 1147, 1199
BspANI GGCC 3 cut(s) 342, 898, 1036
BspCNI CTCAG 2 cut(s) 478, 754
BspFNI CGCG 2 cut(s) 348, 738
BspLI GGNNCC 4 cut(s) 34, 145, 612, 968
BspMAI CTGCAG 2 cut(s) 98, 405
BspPI GGATC 5 cut(s) 27, 40, 56, 605, 618
BsrFI RCCGGY 1 cut(s) 1237
BsrI ACTGG 2 cut(s) 382, 846
BssAI RCCGGY 1 cut(s) 1237
BssECI CCNNGG 2 cut(s) 175, 1134
BssMI GATC 6 cut(s) 32, 48, 109, 598, 610, 758
BssNI GRCGYC 1 cut(s) 11
Bst2UI CCWGG 4 cut(s) 148, 643, 1101, 1136
Bst4CI ACNGT 3 cut(s) 363, 879, 1180
Bst6I CTCTTC 1 cut(s) 176
BstACI GRCGYC 1 cut(s) 11
BstBAI YACGTR 1 cut(s) 1171
BstC8I GCNNGC 2 cut(s) 94, 461
BstDEI CTNAG 3 cut(s) 465, 762, 767
BstENI CCTNNNNNAGG 1 cut(s) 285
BstF5I GGATG 2 cut(s) 264, 1096
BstFNI CGCG 2 cut(s) 348, 738
BstH2I RGCGCY 1 cut(s) 1107
BstHHI GCGC 2 cut(s) 215, 1106
BstKTI GATC 6 cut(s) 35, 51, 112, 601, 613, 761
BstMAI GTCTC 5 cut(s) 133, 217, 240, 400, 590
BstMBI GATC 6 cut(s) 32, 48, 109, 598, 610, 758
BstMCI CGRYCG 2 cut(s) 112, 1203
BstMWI GCNNNNNNNGC 5 cut(s) 98, 754, 763, 1028, 1110
BstNI CCWGG 4 cut(s) 148, 643, 1101, 1136
BstSCI CCNGG 4 cut(s) 146, 641, 1099, 1134
BstSFI CTRYAG 3 cut(s) 94, 401, 454
BstUI CGCG 2 cut(s) 348, 738
BstV1I GCAGC 1 cut(s) 668
BstV2I GAAGAC 1 cut(s) 9
BstX2I RGATCY 3 cut(s) 32, 48, 610
BstYI RGATCY 3 cut(s) 32, 48, 610
BsuI GTATCC 2 cut(s) 558, 844
BsuRI GGCC 3 cut(s) 342, 898, 1036
BtsCI GGATG 2 cut(s) 264, 1096
BtsI GCAGTG 1 cut(s) 899
BtsIMutI CAGTG 3 cut(s) 594, 839, 899
Cac8I GCNNGC 2 cut(s) 94, 461
CaiI CAGNNNCTG 1 cut(s) 409
CfoI GCGC 2 cut(s) 215, 1106
Cfr10I RCCGGY 1 cut(s) 1237
Cfr13I GGNCC 2 cut(s) 966, 1035
CseI GACGC 1 cut(s) 227
Csp6I GTAC 1 cut(s) 1063
CspAI ACCGGT 1 cut(s) 1237
CviAII CATG 6 cut(s) 26, 219, 562, 628, 649, 963
CviQI GTAC 1 cut(s) 1063
DdeI CTNAG 3 cut(s) 465, 762, 767
DpnI GATC 6 cut(s) 34, 50, 111, 600, 612, 760
DpnII GATC 6 cut(s) 32, 48, 109, 598, 610, 758
DraIII CACNNNGTG 1 cut(s) 666
DrdI GACNNNNNNGTC 1 cut(s) 1055
DriI GACNNNNNGTC 1 cut(s) 964
DseDI GACNNNNNNGTC 1 cut(s) 1055
EaeI YGGCCR 1 cut(s) 896
Eam1104I CTCTTC 1 cut(s) 176
Eam1105I GACNNNNNGTC 1 cut(s) 964
EarI CTCTTC 1 cut(s) 176
EciI GGCGGA 1 cut(s) 1162
Eco47I GGWCC 1 cut(s) 966
Eco57I CTGAAG 1 cut(s) 431
Eco72I CACGTG 1 cut(s) 1171
EcoNI CCTNNNNNAGG 1 cut(s) 285
EcoRII CCWGG 4 cut(s) 146, 641, 1099, 1134
FaeI CATG 6 cut(s) 29, 222, 565, 631, 652, 966
FalI AAGNNNNNCTT 2 cut(s) 734, 766
FaqI GGGAC 2 cut(s) 952, 971
FatI CATG 6 cut(s) 25, 218, 561, 627, 648, 962
FauNDI CATATG 1 cut(s) 901
Fnu4HI GCNGC 2 cut(s) 657, 1145
FokI GGATG 2 cut(s) 251, 1083
Fsp4HI GCNGC 2 cut(s) 657, 1145
FspAI RTGCGCAY 1 cut(s) 214
FspBI CTAG 4 cut(s) 489, 710, 954, 977
FspI TGCGCA 1 cut(s) 214
GlaI GCGC 2 cut(s) 214, 1105
GluI GCNGC 2 cut(s) 657, 1145
GsaI CCCAGC 1 cut(s) 817
GsuI CTGGAG 2 cut(s) 399, 746
HaeII RGCGCY 1 cut(s) 1107
HaeIII GGCC 3 cut(s) 342, 898, 1036
HapII CCGG 5 cut(s) 36, 74, 123, 1089, 1238
HgaI GACGC 1 cut(s) 227
HhaI GCGC 2 cut(s) 215, 1106
Hin1I GRCGYC 1 cut(s) 11
Hin1II CATG 6 cut(s) 29, 222, 565, 631, 652, 966
Hin6I GCGC 2 cut(s) 213, 1104
HinP1I GCGC 2 cut(s) 213, 1104
HincII GTYRAC 2 cut(s) 559, 1210
HindII GTYRAC 2 cut(s) 559, 1210
HpaII CCGG 5 cut(s) 36, 74, 123, 1089, 1238
HphI GGTGA 2 cut(s) 50, 112
Hpy166II GTNNAC 2 cut(s) 559, 1210
Hpy188III TCNNGA 5 cut(s) 431, 489, 617, 725, 788
Hpy8I GTNNAC 2 cut(s) 559, 1210
Hpy99I CGWCG 4 cut(s) 13, 16, 110, 1220
HpyAV CCTTC 6 cut(s) 23, 406, 428, 646, 1019, 1063
HpyCH4III ACNGT 3 cut(s) 363, 879, 1180
HpyCH4IV ACGT 2 cut(s) 11, 1170
HpyCH4V TGCA 7 cut(s) 4, 96, 358, 403, 594, 857, 986
HpyF10VI GCNNNNNNNGC 5 cut(s) 98, 754, 763, 1028, 1110
HpyF3I CTNAG 3 cut(s) 465, 762, 767
HpySE526I ACGT 2 cut(s) 11, 1170
Hsp92I GRCGYC 1 cut(s) 11
Hsp92II CATG 6 cut(s) 29, 222, 565, 631, 652, 966
HspAI GCGC 2 cut(s) 213, 1104
Kzo9I GATC 6 cut(s) 32, 48, 109, 598, 610, 758
LmnI GCTCC 5 cut(s) 143, 380, 437, 1110, 1149
Lsp1109I GCAGC 1 cut(s) 668
LweI GCATC 5 cut(s) 224, 345, 436, 735, 1009
MaeI CTAG 4 cut(s) 489, 710, 954, 977
MaeII ACGT 2 cut(s) 11, 1170
MaeIII GTNAC 2 cut(s) 118, 837
MalI GATC 6 cut(s) 34, 50, 111, 600, 612, 760
MboI GATC 6 cut(s) 32, 48, 109, 598, 610, 758
MboII GAAGA 6 cut(s) 9, 193, 299, 364, 1157, 1240
MfeI CAATTG 1 cut(s) 858
MflI RGATCY 3 cut(s) 32, 48, 610
MhlI GDGCHC 1 cut(s) 1185
MlsI TGGCCA 1 cut(s) 898
MluCI AATT 8 cut(s) 671, 799, 858, 872, 948, 1012, 1052, 1188
MluNI TGGCCA 1 cut(s) 898
MlyI GAGTC 4 cut(s) 71, 135, 242, 400
Mox20I TGGCCA 1 cut(s) 898
MroXI GAANNNNTTC 1 cut(s) 686
MscI TGGCCA 1 cut(s) 898
MseI TTAA 1 cut(s) 293
MslI CAYNNNNRTG 1 cut(s) 1240
Msp20I TGGCCA 1 cut(s) 898
MspI CCGG 5 cut(s) 36, 74, 123, 1089, 1238
MspR9I CCNGG 4 cut(s) 148, 643, 1101, 1136
MunI CAATTG 1 cut(s) 858
MvaI CCWGG 4 cut(s) 148, 643, 1101, 1136
MvnI CGCG 2 cut(s) 348, 738
MwoI GCNNNNNNNGC 5 cut(s) 98, 754, 763, 1028, 1110
NdeI CATATG 1 cut(s) 901
NdeII GATC 6 cut(s) 32, 48, 109, 598, 610, 758
NlaIII CATG 6 cut(s) 29, 222, 565, 631, 652, 966
NlaIV GGNNCC 4 cut(s) 34, 145, 612, 968
NmeAIII GCCGAG 1 cut(s) 913
NmuCI GTSAC 2 cut(s) 118, 837
NsbI TGCGCA 1 cut(s) 214
OliI CACNNNNGTG 1 cut(s) 1240
PcsI WCGNNNNNNNCGW 2 cut(s) 1056, 1136
PdmI GAANNNNTTC 1 cut(s) 686
PfeI GAWTC 9 cut(s) 60, 171, 198, 297, 469, 485, 719, 998, 1092
PflFI GACNNNGTC 1 cut(s) 13
PinAI ACCGGT 1 cut(s) 1237
PkrI GCNGC 2 cut(s) 658, 1146
Ple19I CGATCG 1 cut(s) 112
PleI GAGTC 4 cut(s) 71, 134, 242, 400
PmaCI CACGTG 1 cut(s) 1171
PmlI CACGTG 1 cut(s) 1171
PpsI GAGTC 4 cut(s) 71, 134, 242, 400
Ppu21I YACGTR 1 cut(s) 1171
Psp6I CCWGG 4 cut(s) 146, 641, 1099, 1134
PspCI CACGTG 1 cut(s) 1171
PspFI CCCAGC 1 cut(s) 813
PspGI CCWGG 4 cut(s) 146, 641, 1099, 1134
PspN4I GGNNCC 4 cut(s) 34, 145, 612, 968
PspPI GGNCC 2 cut(s) 966, 1035
PstI CTGCAG 2 cut(s) 98, 405
PstNI CAGNNNCTG 1 cut(s) 409
PsuI RGATCY 3 cut(s) 32, 48, 610
PsyI GACNNNGTC 1 cut(s) 13
PvuI CGATCG 1 cut(s) 112
RsaI GTAC 1 cut(s) 1064
RsaNI GTAC 1 cut(s) 1063
RseI CAYNNNNRTG 1 cut(s) 1240
SaqAI TTAA 1 cut(s) 293
SatI GCNGC 2 cut(s) 657, 1145
Sau3AI GATC 6 cut(s) 32, 48, 109, 598, 610, 758
Sau96I GGNCC 2 cut(s) 966, 1035
ScaI AGTACT 1 cut(s) 1064
SchI GAGTC 4 cut(s) 71, 135, 242, 400
ScrFI CCNGG 4 cut(s) 148, 643, 1101, 1136
SduI GDGCHC 1 cut(s) 1185
SfaNI GCATC 5 cut(s) 224, 345, 436, 735, 1009
SfcI CTRYAG 3 cut(s) 94, 401, 454
SgrAI CRCCGGYG 1 cut(s) 1237
SinI GGWCC 1 cut(s) 966
SmiMI CAYNNNNRTG 1 cut(s) 1240
SmlI CTYRAG 2 cut(s) 602, 615
SmoI CTYRAG 2 cut(s) 602, 615
Sse9I AATT 8 cut(s) 671, 799, 858, 872, 948, 1012, 1052, 1188
SsiI CCGC 5 cut(s) 736, 1022, 1144, 1147, 1199
SspMI CTAG 4 cut(s) 489, 710, 954, 977
StyD4I CCNGG 4 cut(s) 146, 641, 1099, 1134
TaaI ACNGT 3 cut(s) 363, 879, 1180
TaiI ACGT 2 cut(s) 14, 1173
TaqI TCGA 6 cut(s) 8, 80, 112, 286, 1059, 1203
TasI AATT 8 cut(s) 671, 799, 858, 872, 948, 1012, 1052, 1188
TatI WGTACW 1 cut(s) 1062
TauI GCSGC 1 cut(s) 1147
TfiI GAWTC 9 cut(s) 60, 171, 198, 297, 469, 485, 719, 998, 1092
Tru1I TTAA 1 cut(s) 293
Tru9I TTAA 1 cut(s) 293
TscAI CASTG 3 cut(s) 601, 846, 899
TseFI GTSAC 2 cut(s) 118, 837
TseI GCWGC 1 cut(s) 656
Tsp45I GTSAC 2 cut(s) 118, 837
TspDTI ATGAA 5 cut(s) 42, 665, 679, 789, 990
TspGWI ACGGA 2 cut(s) 10, 1233
TspRI CASTG 3 cut(s) 601, 846, 899
Tth111I GACNNNGTC 1 cut(s) 13
VpaK11BI GGWCC 1 cut(s) 966
XagI CCTNNNNNAGG 1 cut(s) 285
XapI RAATTY 2 cut(s) 671, 1052
XbaI TCTAGA 1 cut(s) 488
XmnI GAANNNNTTC 1 cut(s) 686
XspI CTAG 4 cut(s) 489, 710, 954, 977
ZraI GACGTC 1 cut(s) 12
ZrmI AGTACT 1 cut(s) 1064
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.