RchiOBHm_Chr5g0024051

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0013210
Physical Location & Seq
Forward (+)
18234300 .. 18239446
5147 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30383

Sequence Viewer

Length: 753 bp
ATGAGCAAGTTGGGTAGAGGCCAACGCGAGAAGATGCAACAGTTTATGACAATAACTGGAGCGAGTGATAAAGTCTCTCTGCAGACTCTGAAAGCGAGTGATTGGCATCTTGAAGGAGCATTTGATATATTCTACAGCCAGCCTCAGATTCAAGCACATACTGATTCTAGACATTTGGAGAAACTATACAATAGATATAAAGATCCAAGTGTTGACATGATACTCGCTGATGGTATCAGTCTCCTTTGCACTGATCTCAAGGTGGATCCTCAAGATATAGTCATGTTAGTTGTTTCCTGGCACATGAAGGCAGCCACCATGTGTGAATTTTCCAAGAATGAGTTCATAGATGGATTAGAAGCACTAGGGATAGATTCTCTGGAGAAGTTCCGCGAAAAGATGCCCTTTATGCGATCTGAGCTAAGAGATGAACAGAAGTTTCGGGAGATATATAATTTTGCTTTTGGCTGGGCAAAAGAAAAGGGTCAGAAGTCACTGGCATTGGATACTGCAATTGGAATGTGGCAATTACTGTTTGCTGAAAAGCAGTGGCCATATGTTGAGCATTGGTGTCAGTTCTTACAGGCTCGGCATAACAAGGCAATTTCTAGGGACACATGGTCCCAACTACTAGAGTTTGCAAGGGCTGTTGATTCATCACTATCAAATTATGATGCGGAAGGTGCTTGGCCCTATCTCATAGACGAATTTGTCGAGTACTTGAAGGAGAATGGCGTAATCAAAAGTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding UBA_4

Protein Analysis

250

Amino Acids

29.06

Weight (kDa)

5.45

Isoelectric Point (pI)

32.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_8 PF22566 8 - 45 9.2e-10 UBA-like domain
UBA_4 PF14555 9 - 48 1.6e-10 UBA-like domain
Cullin_binding PF03556 130 - 240 8.8e-38 Cullin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013210)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12760
fragaria_vesca FvH4_3g14590 FvH4_3g14590
malus_domestica MD05G1234900.v1.1 MD10G1211100.v1.1
prunus_persica Prupe.4G131300_v2.0.a1
pyrus_communis pycom05g21180 pycom10g17940
rosa_chinensis RchiOBHm_Chr5g0024051
rosa_laevigata RLG00000032757
rosa_multiflora Rmu_sc0011761.1_g000007
rosa_roxburghii Rroxscaffold_1G00055190
rosa_rugosa Rorug05G0081200 Rorug05G0081200
rosa_samantha Rh5AG171600 Rh5CG183800 Rh5DG170400
rosa_wichuraiana Rw5G015530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 710
AccII CGCG 2 cut(s) 27, 393
AciI CCGC 2 cut(s) 391, 677
AclWI GGATC 3 cut(s) 197, 260, 273
AcoI YGGCCR 1 cut(s) 551
AcsI RAATTY 2 cut(s) 326, 707
AdeI CACNNNGTG 1 cut(s) 321
AfaI GTAC 1 cut(s) 719
AgsI TTSAA 3 cut(s) 113, 152, 724
AhdI GACNNNNNGTC 1 cut(s) 619
AjnI CCWGG 1 cut(s) 296
AjuI GAANNNNNNNTTGG 2 cut(s) 326, 358
AluBI AGCT 1 cut(s) 421
AluI AGCT 1 cut(s) 421
Alw26I GTCTC 2 cut(s) 79, 245
AlwI GGATC 3 cut(s) 197, 260, 273
AlwNI CAGNNNCTG 1 cut(s) 88
AoxI GGCC 3 cut(s) 19, 551, 689
ApeKI GCWGC 1 cut(s) 311
ApoI RAATTY 2 cut(s) 326, 707
Asp700I GAANNNNTTC 1 cut(s) 341
AspS9I GGNCC 2 cut(s) 621, 690
AvaII GGWCC 1 cut(s) 621
BalI TGGCCA 1 cut(s) 553
BamHI GGATCC 1 cut(s) 265
BbvI GCAGC 1 cut(s) 323
BccI CCATC 2 cut(s) 224, 344
BciT130I CCWGG 1 cut(s) 298
BciVI GTATCC 1 cut(s) 499
BcoDI GTCTC 2 cut(s) 79, 245
BfaI CTAG 4 cut(s) 168, 365, 609, 632
BfmI CTRYAG 2 cut(s) 80, 133
BfuI GTATCC 1 cut(s) 499
BisI GCNGC 1 cut(s) 312
BlsI GCNGC 1 cut(s) 313
BmcAI AGTACT 1 cut(s) 719
Bme1390I CCNGG 1 cut(s) 298
Bme18I GGWCC 1 cut(s) 621
BmeRI GACNNNNNGTC 1 cut(s) 619
BmgT120I GGNCC 2 cut(s) 621, 690
BmiI GGNNCC 2 cut(s) 267, 623
BmrFI CCNGG 1 cut(s) 298
BmsI GCATC 4 cut(s) 24, 115, 390, 664
BpmI CTGGAG 2 cut(s) 78, 401
BpuEI CTTGAG 2 cut(s) 242, 255
BsaBI GATNNNNATC 1 cut(s) 105
Bse1I ACTGG 2 cut(s) 61, 501
Bse8I GATNNNNATC 1 cut(s) 105
BseBI CCWGG 1 cut(s) 298
BseJI GATNNNNATC 1 cut(s) 105
BseMII CTCAG 2 cut(s) 158, 408
BseNI ACTGG 2 cut(s) 61, 501
BseXI GCAGC 1 cut(s) 323
BseYI CCCAGC 1 cut(s) 468
Bsh1236I CGCG 2 cut(s) 27, 393
BshFI GGCC 3 cut(s) 21, 553, 691
BslFI GGGAC 2 cut(s) 607, 626
BsmAI GTCTC 2 cut(s) 79, 245
BsmFI GGGAC 2 cut(s) 607, 626
BsnI GGCC 3 cut(s) 21, 553, 691
Bsp143I GATC 4 cut(s) 202, 253, 265, 413
BspACI CCGC 2 cut(s) 391, 677
BspANI GGCC 3 cut(s) 21, 553, 691
BspCNI CTCAG 2 cut(s) 157, 409
BspFNI CGCG 2 cut(s) 27, 393
BspLI GGNNCC 2 cut(s) 267, 623
BspMAI CTGCAG 1 cut(s) 84
BspPI GGATC 3 cut(s) 197, 260, 273
BsrI ACTGG 2 cut(s) 61, 501
BssMI GATC 4 cut(s) 202, 253, 265, 413
Bst2UI CCWGG 1 cut(s) 298
Bst4CI ACNGT 2 cut(s) 42, 534
BstC8I GCNNGC 1 cut(s) 140
BstDEI CTNAG 3 cut(s) 144, 417, 422
BstFNI CGCG 2 cut(s) 27, 393
BstKTI GATC 4 cut(s) 205, 256, 268, 416
BstMAI GTCTC 2 cut(s) 79, 245
BstMBI GATC 4 cut(s) 202, 253, 265, 413
BstMWI GCNNNNNNNGC 3 cut(s) 409, 418, 683
BstNI CCWGG 1 cut(s) 298
BstSCI CCNGG 1 cut(s) 296
BstSFI CTRYAG 2 cut(s) 80, 133
BstUI CGCG 2 cut(s) 27, 393
BstV1I GCAGC 1 cut(s) 323
BstX2I RGATCY 2 cut(s) 202, 265
BstYI RGATCY 2 cut(s) 202, 265
BsuI GTATCC 1 cut(s) 499
BsuRI GGCC 3 cut(s) 21, 553, 691
BtsI GCAGTG 1 cut(s) 554
BtsIMutI CAGTG 3 cut(s) 249, 494, 554
Cac8I GCNNGC 1 cut(s) 140
CaiI CAGNNNCTG 1 cut(s) 88
Cfr13I GGNCC 2 cut(s) 621, 690
Csp6I GTAC 1 cut(s) 718
CviAII CATG 5 cut(s) 217, 283, 304, 319, 618
CviQI GTAC 1 cut(s) 718
DdeI CTNAG 3 cut(s) 144, 417, 422
DpnI GATC 4 cut(s) 204, 255, 267, 415
DpnII GATC 4 cut(s) 202, 253, 265, 413
DraIII CACNNNGTG 1 cut(s) 321
DrdI GACNNNNNNGTC 1 cut(s) 710
DriI GACNNNNNGTC 1 cut(s) 619
DseDI GACNNNNNNGTC 1 cut(s) 710
EaeI YGGCCR 1 cut(s) 551
Eam1105I GACNNNNNGTC 1 cut(s) 619
Eco47I GGWCC 1 cut(s) 621
EcoRII CCWGG 1 cut(s) 296
FaeI CATG 5 cut(s) 220, 286, 307, 322, 621
FalI AAGNNNNNCTT 2 cut(s) 389, 421
FaqI GGGAC 2 cut(s) 607, 626
FatI CATG 5 cut(s) 216, 282, 303, 318, 617
FauNDI CATATG 1 cut(s) 556
Fnu4HI GCNGC 1 cut(s) 312
Fsp4HI GCNGC 1 cut(s) 312
FspBI CTAG 4 cut(s) 168, 365, 609, 632
GluI GCNGC 1 cut(s) 312
GsaI CCCAGC 1 cut(s) 472
GsuI CTGGAG 2 cut(s) 78, 401
HaeIII GGCC 3 cut(s) 21, 553, 691
Hin1II CATG 5 cut(s) 220, 286, 307, 322, 621
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HinfI GANTC 5 cut(s) 85, 148, 164, 374, 653
Hpy166II GTNNAC 1 cut(s) 214
Hpy188I TCNGA 4 cut(s) 90, 147, 418, 489
Hpy188III TCNNGA 5 cut(s) 110, 168, 272, 380, 443
Hpy8I GTNNAC 1 cut(s) 214
HpyAV CCTTC 4 cut(s) 107, 301, 674, 718
HpyCH4III ACNGT 2 cut(s) 42, 534
HpyCH4V TGCA 5 cut(s) 37, 82, 249, 512, 641
HpyF10VI GCNNNNNNNGC 3 cut(s) 409, 418, 683
HpyF3I CTNAG 3 cut(s) 144, 417, 422
Hsp92II CATG 5 cut(s) 220, 286, 307, 322, 621
Kzo9I GATC 4 cut(s) 202, 253, 265, 413
LmnI GCTCC 2 cut(s) 59, 116
LpnPI CCDG 8 cut(s) 42, 152, 283, 310, 365, 454, 482, 569
Lsp1109I GCAGC 1 cut(s) 323
LweI GCATC 4 cut(s) 24, 115, 390, 664
MaeI CTAG 4 cut(s) 168, 365, 609, 632
MaeIII GTNAC 1 cut(s) 492
MalI GATC 4 cut(s) 204, 255, 267, 415
MboI GATC 4 cut(s) 202, 253, 265, 413
MboII GAAGA 1 cut(s) 43
MfeI CAATTG 1 cut(s) 513
MflI RGATCY 2 cut(s) 202, 265
MlsI TGGCCA 1 cut(s) 553
MluCI AATT 7 cut(s) 326, 454, 513, 527, 603, 667, 707
MluNI TGGCCA 1 cut(s) 553
MlyI GAGTC 1 cut(s) 79
MnlI CCTC 3 cut(s) 11, 153, 279
Mox20I TGGCCA 1 cut(s) 553
MroXI GAANNNNTTC 1 cut(s) 341
MscI TGGCCA 1 cut(s) 553
Msp20I TGGCCA 1 cut(s) 553
MspR9I CCNGG 1 cut(s) 298
MunI CAATTG 1 cut(s) 513
MvaI CCWGG 1 cut(s) 298
MvnI CGCG 2 cut(s) 27, 393
MwoI GCNNNNNNNGC 3 cut(s) 409, 418, 683
NdeI CATATG 1 cut(s) 556
NdeII GATC 4 cut(s) 202, 253, 265, 413
NlaIII CATG 5 cut(s) 220, 286, 307, 322, 621
NlaIV GGNNCC 2 cut(s) 267, 623
NmeAIII GCCGAG 1 cut(s) 568
NmuCI GTSAC 1 cut(s) 492
PcsI WCGNNNNNNNCGW 1 cut(s) 711
PdmI GAANNNNTTC 1 cut(s) 341
PfeI GAWTC 4 cut(s) 148, 164, 374, 653
PkrI GCNGC 1 cut(s) 313
PleI GAGTC 1 cut(s) 79
PpsI GAGTC 1 cut(s) 79
Psp6I CCWGG 1 cut(s) 296
PspFI CCCAGC 1 cut(s) 468
PspGI CCWGG 1 cut(s) 296
PspN4I GGNNCC 2 cut(s) 267, 623
PspPI GGNCC 2 cut(s) 621, 690
PstI CTGCAG 1 cut(s) 84
PstNI CAGNNNCTG 1 cut(s) 88
PsuI RGATCY 2 cut(s) 202, 265
RsaI GTAC 1 cut(s) 719
RsaNI GTAC 1 cut(s) 718
SatI GCNGC 1 cut(s) 312
Sau3AI GATC 4 cut(s) 202, 253, 265, 413
Sau96I GGNCC 2 cut(s) 621, 690
ScaI AGTACT 1 cut(s) 719
SchI GAGTC 1 cut(s) 79
ScrFI CCNGG 1 cut(s) 298
SetI ASST 3 cut(s) 264, 423, 685
SfaNI GCATC 4 cut(s) 24, 115, 390, 664
SfcI CTRYAG 2 cut(s) 80, 133
SinI GGWCC 1 cut(s) 621
SmlI CTYRAG 2 cut(s) 257, 270
SmoI CTYRAG 2 cut(s) 257, 270
Sse9I AATT 7 cut(s) 326, 454, 513, 527, 603, 667, 707
SsiI CCGC 2 cut(s) 391, 677
SspMI CTAG 4 cut(s) 168, 365, 609, 632
StyD4I CCNGG 1 cut(s) 296
TaaI ACNGT 2 cut(s) 42, 534
TaqI TCGA 1 cut(s) 714
TasI AATT 7 cut(s) 326, 454, 513, 527, 603, 667, 707
TatI WGTACW 1 cut(s) 717
TfiI GAWTC 4 cut(s) 148, 164, 374, 653
TscAI CASTG 3 cut(s) 256, 501, 554
TseFI GTSAC 1 cut(s) 492
TseI GCWGC 1 cut(s) 311
Tsp45I GTSAC 1 cut(s) 492
TspDTI ATGAA 4 cut(s) 320, 334, 444, 645
TspRI CASTG 3 cut(s) 256, 501, 554
VpaK11BI GGWCC 1 cut(s) 621
XapI RAATTY 2 cut(s) 326, 707
XbaI TCTAGA 1 cut(s) 167
XmnI GAANNNNTTC 1 cut(s) 341
XspI CTAG 4 cut(s) 168, 365, 609, 632
ZrmI AGTACT 1 cut(s) 719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.