Rh5AG171600

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0013210
Physical Location & Seq
Forward (+)
18969282 .. 18971629
2348 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG171600.1

Sequence Viewer

Length: 705 bp
ATGCTGCTTAACTATATCACACCTTCAAATGTATGCTTTGTTATTTGCTATAATGTTCCACCTATATTGAATAATTTTTTCACATTTTTATTTTATTACTTTTTTTATGAGCTCTGCATAAGTTCCTTTTTACTAATGAAGTACTCTTTTCCAGATCCAAGTGTTGACATGATACTTGCTGATGGTATCAGTCTCCTTTGCACTGATCTCAAGGTGGATCCTCAAGATATAGTCATGTTAGTTGTTTCCTGGCACATGAAGGCAGCCACCATGTGTGAATTTTCTAAGAATGAGTTCATAGATGGATTAGAAGCACTAGGGATAGATTCTCTGGAGAAGTTCCGCGAAAAGATGCCCTTTATGCGATCTGAGCTAAGAGATGAACAGAAGTTTCGGGAGATATATAATTTTGCTTTTGGCTGGGCAAAAGAAAAGGGTCAGAAGTCACTGGCATTGGATACTGCAATTGGAATGTGGCAATTACTGTTTGCTGAAAAGCAGTGGCCATATGTTGAGCATTGGTGTCAGTTCTTACAGGCTCGGCATAACAAGGCAATTTCTAGGGACACATGGTCCCAACTACTAGAGTTTGCAAGGGCTGTTGATTCATCACTATCAAATTATGACGCGGAAGGTGCTTGGCCCTATCTCATAGACGAATTTGTCGAGTACTTGAAGGAGAATGGCGTAATCAAAAGTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding UBA_4

Protein Analysis

234

Amino Acids

27.42

Weight (kDa)

4.82

Isoelectric Point (pI)

33.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin_binding PF03556 114 - 224 7.5e-38 Cullin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013210)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12760
fragaria_vesca FvH4_3g14590 FvH4_3g14590
malus_domestica MD05G1234900.v1.1 MD10G1211100.v1.1
prunus_persica Prupe.4G131300_v2.0.a1
pyrus_communis pycom05g21180 pycom10g17940
rosa_chinensis RchiOBHm_Chr5g0024051
rosa_laevigata RLG00000032757
rosa_multiflora Rmu_sc0011761.1_g000007
rosa_roxburghii Rroxscaffold_1G00055190
rosa_rugosa Rorug05G0081200 Rorug05G0081200
rosa_samantha Rh5AG171600 Rh5CG183800 Rh5DG170400
rosa_wichuraiana Rw5G015530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 662
AccII CGCG 2 cut(s) 345, 629
AciI CCGC 2 cut(s) 343, 629
AclWI GGATC 3 cut(s) 149, 212, 225
AcoI YGGCCR 1 cut(s) 503
AcsI RAATTY 2 cut(s) 278, 659
AdeI CACNNNGTG 1 cut(s) 273
AfaI GTAC 2 cut(s) 143, 671
AgsI TTSAA 3 cut(s) 27, 70, 676
AhdI GACNNNNNGTC 1 cut(s) 571
AjnI CCWGG 1 cut(s) 248
AluBI AGCT 2 cut(s) 112, 373
AluI AGCT 2 cut(s) 112, 373
Alw21I GWGCWC 1 cut(s) 114
Alw26I GTCTC 1 cut(s) 197
AlwI GGATC 3 cut(s) 149, 212, 225
AoxI GGCC 2 cut(s) 503, 641
ApeKI GCWGC 2 cut(s) 4, 263
ApoI RAATTY 2 cut(s) 278, 659
Asp700I GAANNNNTTC 1 cut(s) 293
AspS9I GGNCC 2 cut(s) 573, 642
AvaII GGWCC 1 cut(s) 573
BalI TGGCCA 1 cut(s) 505
BamHI GGATCC 1 cut(s) 217
BanII GRGCYC 1 cut(s) 114
Bbv12I GWGCWC 1 cut(s) 114
BbvI GCAGC 1 cut(s) 275
BccI CCATC 2 cut(s) 176, 296
BciT130I CCWGG 1 cut(s) 250
BciVI GTATCC 1 cut(s) 451
BcoDI GTCTC 1 cut(s) 197
BfaI CTAG 3 cut(s) 317, 561, 584
BfuI GTATCC 1 cut(s) 451
BisI GCNGC 2 cut(s) 5, 264
BlsI GCNGC 2 cut(s) 6, 265
BmcAI AGTACT 2 cut(s) 143, 671
Bme1390I CCNGG 1 cut(s) 250
Bme18I GGWCC 1 cut(s) 573
BmeRI GACNNNNNGTC 1 cut(s) 571
BmgT120I GGNCC 2 cut(s) 573, 642
BmiI GGNNCC 2 cut(s) 219, 575
BmrFI CCNGG 1 cut(s) 250
BmsI GCATC 1 cut(s) 342
BpmI CTGGAG 1 cut(s) 353
BpuEI CTTGAG 2 cut(s) 194, 207
Bse1I ACTGG 1 cut(s) 453
BseBI CCWGG 1 cut(s) 250
BseMII CTCAG 1 cut(s) 360
BseNI ACTGG 1 cut(s) 453
BseXI GCAGC 1 cut(s) 275
BseYI CCCAGC 1 cut(s) 420
Bsh1236I CGCG 2 cut(s) 345, 629
BshFI GGCC 2 cut(s) 505, 643
BsiHKAI GWGCWC 1 cut(s) 114
BslFI GGGAC 2 cut(s) 559, 578
BsmAI GTCTC 1 cut(s) 197
BsmFI GGGAC 2 cut(s) 559, 578
BsnI GGCC 2 cut(s) 505, 643
Bsp1286I GDGCHC 1 cut(s) 114
Bsp143I GATC 4 cut(s) 154, 205, 217, 365
BspACI CCGC 2 cut(s) 343, 629
BspANI GGCC 2 cut(s) 505, 643
BspCNI CTCAG 1 cut(s) 361
BspFNI CGCG 2 cut(s) 345, 629
BspLI GGNNCC 2 cut(s) 219, 575
BspPI GGATC 3 cut(s) 149, 212, 225
BsrI ACTGG 1 cut(s) 453
BssMI GATC 4 cut(s) 154, 205, 217, 365
Bst2UI CCWGG 1 cut(s) 250
Bst4CI ACNGT 1 cut(s) 486
BstDEI CTNAG 3 cut(s) 285, 369, 374
BstFNI CGCG 2 cut(s) 345, 629
BstKTI GATC 4 cut(s) 157, 208, 220, 368
BstMAI GTCTC 1 cut(s) 197
BstMBI GATC 4 cut(s) 154, 205, 217, 365
BstMWI GCNNNNNNNGC 3 cut(s) 361, 370, 635
BstNI CCWGG 1 cut(s) 250
BstSCI CCNGG 1 cut(s) 248
BstUI CGCG 2 cut(s) 345, 629
BstV1I GCAGC 1 cut(s) 275
BstX2I RGATCY 2 cut(s) 154, 217
BstYI RGATCY 2 cut(s) 154, 217
BsuI GTATCC 1 cut(s) 451
BsuRI GGCC 2 cut(s) 505, 643
BtsI GCAGTG 1 cut(s) 506
BtsIMutI CAGTG 3 cut(s) 201, 446, 506
Cfr13I GGNCC 2 cut(s) 573, 642
CseI GACGC 1 cut(s) 635
Csp6I GTAC 2 cut(s) 142, 670
CspCI CAANNNNNGTGG 2 cut(s) 48, 83
CviAII CATG 5 cut(s) 169, 235, 256, 271, 570
CviJI RGCY 8 cut(s) 112, 266, 373, 420, 505, 539, 599, 643
CviKI_1 RGCY 8 cut(s) 112, 266, 373, 420, 505, 539, 599, 643
CviQI GTAC 2 cut(s) 142, 670
DdeI CTNAG 3 cut(s) 285, 369, 374
DpnI GATC 4 cut(s) 156, 207, 219, 367
DpnII GATC 4 cut(s) 154, 205, 217, 365
DraIII CACNNNGTG 1 cut(s) 273
DrdI GACNNNNNNGTC 1 cut(s) 662
DriI GACNNNNNGTC 1 cut(s) 571
DseDI GACNNNNNNGTC 1 cut(s) 662
EaeI YGGCCR 1 cut(s) 503
Eam1105I GACNNNNNGTC 1 cut(s) 571
Ecl136II GAGCTC 1 cut(s) 112
Eco24I GRGCYC 1 cut(s) 114
Eco47I GGWCC 1 cut(s) 573
Eco53kI GAGCTC 1 cut(s) 112
EcoICRI GAGCTC 1 cut(s) 112
EcoRII CCWGG 1 cut(s) 248
EcoT38I GRGCYC 1 cut(s) 114
FaeI CATG 5 cut(s) 172, 238, 259, 274, 573
FalI AAGNNNNNCTT 2 cut(s) 341, 373
FaqI GGGAC 2 cut(s) 559, 578
FatI CATG 5 cut(s) 168, 234, 255, 270, 569
FauNDI CATATG 1 cut(s) 508
Fnu4HI GCNGC 2 cut(s) 5, 264
FriOI GRGCYC 1 cut(s) 114
Fsp4HI GCNGC 2 cut(s) 5, 264
FspBI CTAG 3 cut(s) 317, 561, 584
GluI GCNGC 2 cut(s) 5, 264
GsaI CCCAGC 1 cut(s) 424
GsuI CTGGAG 1 cut(s) 353
HaeIII GGCC 2 cut(s) 505, 643
HgaI GACGC 1 cut(s) 635
Hin1II CATG 5 cut(s) 172, 238, 259, 274, 573
HincII GTYRAC 1 cut(s) 166
HindII GTYRAC 1 cut(s) 166
HinfI GANTC 2 cut(s) 326, 605
Hpy166II GTNNAC 1 cut(s) 166
Hpy188I TCNGA 2 cut(s) 370, 441
Hpy188III TCNNGA 4 cut(s) 152, 224, 332, 395
Hpy8I GTNNAC 1 cut(s) 166
HpyAV CCTTC 4 cut(s) 33, 253, 626, 670
HpyCH4III ACNGT 1 cut(s) 486
HpyCH4V TGCA 4 cut(s) 117, 201, 464, 593
HpyF10VI GCNNNNNNNGC 3 cut(s) 361, 370, 635
HpyF3I CTNAG 3 cut(s) 285, 369, 374
Hsp92II CATG 5 cut(s) 172, 238, 259, 274, 573
Kzo9I GATC 4 cut(s) 154, 205, 217, 365
LpnPI CCDG 7 cut(s) 165, 235, 262, 317, 406, 434, 521
Lsp1109I GCAGC 1 cut(s) 275
LweI GCATC 1 cut(s) 342
MaeI CTAG 3 cut(s) 317, 561, 584
MaeIII GTNAC 1 cut(s) 444
MalI GATC 4 cut(s) 156, 207, 219, 367
MboI GATC 4 cut(s) 154, 205, 217, 365
MfeI CAATTG 1 cut(s) 465
MflI RGATCY 2 cut(s) 154, 217
MhlI GDGCHC 1 cut(s) 114
MlsI TGGCCA 1 cut(s) 505
MluCI AATT 8 cut(s) 73, 278, 406, 465, 479, 555, 619, 659
MluNI TGGCCA 1 cut(s) 505
MnlI CCTC 1 cut(s) 231
Mox20I TGGCCA 1 cut(s) 505
MroXI GAANNNNTTC 1 cut(s) 293
MscI TGGCCA 1 cut(s) 505
MseI TTAA 1 cut(s) 9
Msp20I TGGCCA 1 cut(s) 505
MspR9I CCNGG 1 cut(s) 250
MunI CAATTG 1 cut(s) 465
MvaI CCWGG 1 cut(s) 250
MvnI CGCG 2 cut(s) 345, 629
MwoI GCNNNNNNNGC 3 cut(s) 361, 370, 635
NdeI CATATG 1 cut(s) 508
NdeII GATC 4 cut(s) 154, 205, 217, 365
NlaIII CATG 5 cut(s) 172, 238, 259, 274, 573
NlaIV GGNNCC 2 cut(s) 219, 575
NmeAIII GCCGAG 1 cut(s) 520
NmuCI GTSAC 1 cut(s) 444
PcsI WCGNNNNNNNCGW 1 cut(s) 663
PdmI GAANNNNTTC 1 cut(s) 293
PfeI GAWTC 2 cut(s) 326, 605
PkrI GCNGC 2 cut(s) 6, 265
Psp124BI GAGCTC 1 cut(s) 114
Psp6I CCWGG 1 cut(s) 248
PspFI CCCAGC 1 cut(s) 420
PspGI CCWGG 1 cut(s) 248
PspN4I GGNNCC 2 cut(s) 219, 575
PspPI GGNCC 2 cut(s) 573, 642
PsuI RGATCY 2 cut(s) 154, 217
RsaI GTAC 2 cut(s) 143, 671
RsaNI GTAC 2 cut(s) 142, 670
SacI GAGCTC 1 cut(s) 114
SaqAI TTAA 1 cut(s) 9
SatI GCNGC 2 cut(s) 5, 264
Sau3AI GATC 4 cut(s) 154, 205, 217, 365
Sau96I GGNCC 2 cut(s) 573, 642
ScaI AGTACT 2 cut(s) 143, 671
ScrFI CCNGG 1 cut(s) 250
SduI GDGCHC 1 cut(s) 114
SetI ASST 6 cut(s) 25, 64, 114, 216, 375, 637
SfaNI GCATC 1 cut(s) 342
SinI GGWCC 1 cut(s) 573
SmlI CTYRAG 2 cut(s) 209, 222
SmoI CTYRAG 2 cut(s) 209, 222
Sse9I AATT 8 cut(s) 73, 278, 406, 465, 479, 555, 619, 659
SsiI CCGC 2 cut(s) 343, 629
SspMI CTAG 3 cut(s) 317, 561, 584
SstI GAGCTC 1 cut(s) 114
StyD4I CCNGG 1 cut(s) 248
TaaI ACNGT 1 cut(s) 486
TaqI TCGA 1 cut(s) 666
TasI AATT 8 cut(s) 73, 278, 406, 465, 479, 555, 619, 659
TatI WGTACW 2 cut(s) 141, 669
TfiI GAWTC 2 cut(s) 326, 605
Tru1I TTAA 1 cut(s) 9
Tru9I TTAA 1 cut(s) 9
TscAI CASTG 3 cut(s) 208, 453, 506
TseFI GTSAC 1 cut(s) 444
TseI GCWGC 2 cut(s) 4, 263
Tsp45I GTSAC 1 cut(s) 444
TspDTI ATGAA 5 cut(s) 152, 272, 286, 396, 597
TspRI CASTG 3 cut(s) 208, 453, 506
VpaK11BI GGWCC 1 cut(s) 573
XapI RAATTY 2 cut(s) 278, 659
XmnI GAANNNNTTC 1 cut(s) 293
XspI CTAG 3 cut(s) 317, 561, 584
ZrmI AGTACT 2 cut(s) 143, 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.