MD05G1234900.v1.1

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0013210
Physical Location & Seq
Reverse (-)
36701794 .. 36706798
5005 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1234900.v1.1.491

Sequence Viewer

Length: 780 bp
ATGCACAAGTCGGGTAGAGGCCAACGTGAAAAGCTTCAGCAGTTCATCATGATAACCGGAGCGAGTGAAAAAGTGGCTTTGCAGGCTCTGAAGTCTAGTGATTGGCATCTTGAAGGAGCGTTTGATGTATTCTACAGTCAGCCCCAGCTGCAAGCACATACTGATTCTAGGCATTTGGAGGAATTATACAATAGATATAAAGGCAAGCTTTACCTTTCCATTTACTCTTATCCACGTGCTGACATGATACTTGTTGATGGTATCAGCCTCCTTTGCAATGATCTTCAGGTGGATCCTCAAGATATCGTCATGTTAGTTGTTTCCTGGCACATGAAGGCAGCTACCATGTGTGAATTTTCCAAGCAGGAGTTCGTAGATGGATTAGAATCACTAGGGATAGATTCTTTGGACAAGTTCCGTGAAAAGATACCATTTATGAGATCCGAGCTGAAAGATGAACAAAAGTTTCGAGAGATATATAATTTTGCTTTTGGCTGGGCAAAAGAAAAGGGTCAGAAATCTTTGGCATTGGATACTGCAATTGGAATGTGGCAATTACTGTTTGCTGAAAAGCAGTGGCCGTATGTTGAACATTGGTGTCAGTTCTTACAGGCTCGGCATAACAAGGCAATTTCGAGGGACACATGGTCTCAACTATTGGAGTTTGCGAGGGCTGTGGATTCATCACTGTCAAACTATGATGCGGAAGGTGCCTGGCCATATCTTATTGACGAATTTGTCGAGTACTTGAAGGAGAACGGCGTAATCCAAAAGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding UBA_4

Protein Analysis

260

Amino Acids

30.17

Weight (kDa)

5.56

Isoelectric Point (pI)

33.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_8 PF22566 8 - 45 1.4e-10 UBA-like domain
UBA_4 PF14555 9 - 48 9.3e-13 UBA-like domain
Cullin_binding PF03556 139 - 249 5.8e-38 Cullin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013210)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12760
fragaria_vesca FvH4_3g14590 FvH4_3g14590
malus_domestica MD05G1234900.v1.1 MD10G1211100.v1.1
prunus_persica Prupe.4G131300_v2.0.a1
pyrus_communis pycom05g21180 pycom10g17940
rosa_chinensis RchiOBHm_Chr5g0024051
rosa_laevigata RLG00000032757
rosa_multiflora Rmu_sc0011761.1_g000007
rosa_roxburghii Rroxscaffold_1G00055190
rosa_rugosa Rorug05G0081200 Rorug05G0081200
rosa_samantha Rh5AG171600 Rh5CG183800 Rh5DG170400
rosa_wichuraiana Rw5G015530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 737
AccB1I GGYRCC 1 cut(s) 710
AciI CCGC 1 cut(s) 704
AclWI GGATC 3 cut(s) 287, 300, 435
AcoI YGGCCR 2 cut(s) 578, 716
AcsI RAATTY 2 cut(s) 353, 734
AcuI CTGAAG 3 cut(s) 20, 110, 269
AcvI CACGTG 1 cut(s) 236
AfaI GTAC 1 cut(s) 746
AgsI TTSAA 3 cut(s) 113, 590, 751
AhdI GACNNNNNGTC 1 cut(s) 646
AjnI CCWGG 2 cut(s) 323, 713
AjuI GAANNNNNNNTTGG 2 cut(s) 353, 385
AluBI AGCT 5 cut(s) 34, 148, 208, 341, 448
AluI AGCT 5 cut(s) 34, 148, 208, 341, 448
Alw26I GTCTC 1 cut(s) 654
AlwI GGATC 3 cut(s) 287, 300, 435
AlwNI CAGNNNCTG 1 cut(s) 88
AoxI GGCC 3 cut(s) 19, 578, 716
ApeKI GCWGC 2 cut(s) 148, 338
ApoI RAATTY 2 cut(s) 353, 734
Asp700I GAANNNNTTC 1 cut(s) 33
BalI TGGCCA 1 cut(s) 718
BamHI GGATCC 1 cut(s) 292
BanI GGYRCC 1 cut(s) 710
BbrPI CACGTG 1 cut(s) 236
BbvI GCAGC 2 cut(s) 135, 350
BccI CCATC 2 cut(s) 251, 371
BceAI ACGGC 2 cut(s) 565, 775
BciT130I CCWGG 2 cut(s) 325, 715
BciVI GTATCC 1 cut(s) 526
BcoDI GTCTC 1 cut(s) 654
BfaI CTAG 3 cut(s) 96, 168, 392
BfmI CTRYAG 1 cut(s) 133
BfuI GTATCC 1 cut(s) 526
BisI GCNGC 2 cut(s) 149, 339
BlsI GCNGC 2 cut(s) 150, 340
BmcAI AGTACT 1 cut(s) 746
Bme1390I CCNGG 2 cut(s) 325, 715
BmeRI GACNNNNNGTC 1 cut(s) 646
BmiI GGNNCC 2 cut(s) 294, 712
BmrFI CCNGG 2 cut(s) 325, 715
BmsI GCATC 2 cut(s) 115, 691
BpuEI CTTGAG 1 cut(s) 282
BsaAI YACGTR 1 cut(s) 236
BsaBI GATNNNNATC 2 cut(s) 105, 385
BsaI GGTCTC 1 cut(s) 654
BsaWI WCCGGW 1 cut(s) 56
Bse3DI GCAATG 1 cut(s) 283
Bse8I GATNNNNATC 2 cut(s) 105, 385
BseBI CCWGG 2 cut(s) 325, 715
BseJI GATNNNNATC 2 cut(s) 105, 385
BseMI GCAATG 1 cut(s) 283
BseXI GCAGC 2 cut(s) 135, 350
BseYI CCCAGC 2 cut(s) 144, 495
BshFI GGCC 3 cut(s) 21, 580, 718
BshNI GGYRCC 1 cut(s) 710
BsiSI CCGG 1 cut(s) 57
BslFI GGGAC 1 cut(s) 653
BsmAI GTCTC 1 cut(s) 654
BsmFI GGGAC 1 cut(s) 653
BsnI GGCC 3 cut(s) 21, 580, 718
Bso31I GGTCTC 1 cut(s) 654
Bsp143I GATC 3 cut(s) 280, 292, 440
BspACI CCGC 1 cut(s) 704
BspANI GGCC 3 cut(s) 21, 580, 718
BspHI TCATGA 1 cut(s) 48
BspLI GGNNCC 2 cut(s) 294, 712
BspPI GGATC 3 cut(s) 287, 300, 435
BspT107I GGYRCC 1 cut(s) 710
BspTNI GGTCTC 1 cut(s) 654
BsrDI GCAATG 1 cut(s) 283
BssMI GATC 3 cut(s) 280, 292, 440
Bst2UI CCWGG 2 cut(s) 325, 715
Bst4CI ACNGT 3 cut(s) 137, 561, 690
BstBAI YACGTR 1 cut(s) 236
BstC8I GCNNGC 3 cut(s) 84, 153, 206
BstKTI GATC 3 cut(s) 283, 295, 443
BstMAI GTCTC 1 cut(s) 654
BstMBI GATC 3 cut(s) 280, 292, 440
BstMWI GCNNNNNNNGC 4 cut(s) 83, 148, 273, 710
BstNI CCWGG 2 cut(s) 325, 715
BstSCI CCNGG 2 cut(s) 323, 713
BstSFI CTRYAG 1 cut(s) 133
BstV1I GCAGC 2 cut(s) 135, 350
BstX2I RGATCY 2 cut(s) 292, 440
BstYI RGATCY 2 cut(s) 292, 440
BsuI GTATCC 1 cut(s) 526
BsuRI GGCC 3 cut(s) 21, 580, 718
BtsI GCAGTG 1 cut(s) 581
BtsIMutI CAGTG 2 cut(s) 581, 686
Cac8I GCNNGC 3 cut(s) 84, 153, 206
CaiI CAGNNNCTG 1 cut(s) 88
CciI TCATGA 1 cut(s) 48
Csp6I GTAC 1 cut(s) 745
CviAII CATG 6 cut(s) 49, 244, 310, 331, 346, 645
CviQI GTAC 1 cut(s) 745
DpnI GATC 3 cut(s) 282, 294, 442
DpnII GATC 3 cut(s) 280, 292, 440
DrdI GACNNNNNNGTC 1 cut(s) 737
DriI GACNNNNNGTC 1 cut(s) 646
DseDI GACNNNNNNGTC 1 cut(s) 737
EaeI YGGCCR 2 cut(s) 578, 716
Eam1105I GACNNNNNGTC 1 cut(s) 646
Eco31I GGTCTC 1 cut(s) 654
Eco32I GATATC 1 cut(s) 304
Eco57I CTGAAG 3 cut(s) 20, 110, 269
Eco72I CACGTG 1 cut(s) 236
EcoRII CCWGG 2 cut(s) 323, 713
EcoRV GATATC 1 cut(s) 304
FaeI CATG 6 cut(s) 52, 247, 313, 334, 349, 648
FalI AAGNNNNNCTT 2 cut(s) 192, 224
FaqI GGGAC 1 cut(s) 653
FatI CATG 6 cut(s) 48, 243, 309, 330, 345, 644
Fnu4HI GCNGC 2 cut(s) 149, 339
Fsp4HI GCNGC 2 cut(s) 149, 339
FspBI CTAG 3 cut(s) 96, 168, 392
GluI GCNGC 2 cut(s) 149, 339
GsaI CCCAGC 2 cut(s) 148, 499
HaeIII GGCC 3 cut(s) 21, 580, 718
HapII CCGG 1 cut(s) 57
Hin1II CATG 6 cut(s) 52, 247, 313, 334, 349, 648
HindIII AAGCTT 2 cut(s) 32, 206
HinfI GANTC 4 cut(s) 164, 386, 401, 680
HpaII CCGG 1 cut(s) 57
Hpy188I TCNGA 3 cut(s) 90, 445, 516
Hpy188III TCNNGA 4 cut(s) 49, 110, 299, 470
HpyAV CCTTC 4 cut(s) 107, 328, 701, 745
HpyCH4III ACNGT 3 cut(s) 137, 561, 690
HpyCH4IV ACGT 2 cut(s) 25, 235
HpyCH4V TGCA 5 cut(s) 4, 82, 151, 276, 539
HpyF10VI GCNNNNNNNGC 4 cut(s) 83, 148, 273, 710
HpySE526I ACGT 2 cut(s) 25, 235
Hsp92II CATG 6 cut(s) 52, 247, 313, 334, 349, 648
Kzo9I GATC 3 cut(s) 280, 292, 440
LmnI GCTCC 2 cut(s) 59, 116
Lsp1109I GCAGC 2 cut(s) 135, 350
LweI GCATC 2 cut(s) 115, 691
MaeI CTAG 3 cut(s) 96, 168, 392
MaeII ACGT 2 cut(s) 25, 235
MalI GATC 3 cut(s) 282, 294, 442
MboI GATC 3 cut(s) 280, 292, 440
MboII GAAGA 1 cut(s) 275
MfeI CAATTG 1 cut(s) 540
MflI RGATCY 2 cut(s) 292, 440
MlsI TGGCCA 1 cut(s) 718
MluCI AATT 7 cut(s) 182, 353, 481, 540, 554, 630, 734
MluNI TGGCCA 1 cut(s) 718
MnlI CCTC 6 cut(s) 11, 172, 278, 306, 630, 663
Mox20I TGGCCA 1 cut(s) 718
MroXI GAANNNNTTC 1 cut(s) 33
MscI TGGCCA 1 cut(s) 718
MseI TTAA 1 cut(s) 778
Msp20I TGGCCA 1 cut(s) 718
MspA1I CMGCKG 1 cut(s) 148
MspI CCGG 1 cut(s) 57
MspR9I CCNGG 2 cut(s) 325, 715
MunI CAATTG 1 cut(s) 540
MvaI CCWGG 2 cut(s) 325, 715
MwoI GCNNNNNNNGC 4 cut(s) 83, 148, 273, 710
NdeII GATC 3 cut(s) 280, 292, 440
NlaIII CATG 6 cut(s) 52, 247, 313, 334, 349, 648
NlaIV GGNNCC 2 cut(s) 294, 712
NmeAIII GCCGAG 1 cut(s) 595
PagI TCATGA 1 cut(s) 48
PcsI WCGNNNNNNNCGW 1 cut(s) 738
PdmI GAANNNNTTC 1 cut(s) 33
PfeI GAWTC 4 cut(s) 164, 386, 401, 680
PkrI GCNGC 2 cut(s) 150, 340
PmaCI CACGTG 1 cut(s) 236
PmlI CACGTG 1 cut(s) 236
Ppu21I YACGTR 1 cut(s) 236
Psp6I CCWGG 2 cut(s) 323, 713
PspCI CACGTG 1 cut(s) 236
PspFI CCCAGC 2 cut(s) 144, 495
PspGI CCWGG 2 cut(s) 323, 713
PspN4I GGNNCC 2 cut(s) 294, 712
PstNI CAGNNNCTG 1 cut(s) 88
PsuI RGATCY 2 cut(s) 292, 440
PvuII CAGCTG 1 cut(s) 148
RsaI GTAC 1 cut(s) 746
RsaNI GTAC 1 cut(s) 745
SaqAI TTAA 1 cut(s) 778
SatI GCNGC 2 cut(s) 149, 339
Sau3AI GATC 3 cut(s) 280, 292, 440
ScaI AGTACT 1 cut(s) 746
ScrFI CCNGG 2 cut(s) 325, 715
SfaNI GCATC 2 cut(s) 115, 691
SfcI CTRYAG 1 cut(s) 133
SmlI CTYRAG 1 cut(s) 297
SmoI CTYRAG 1 cut(s) 297
Sse9I AATT 7 cut(s) 182, 353, 481, 540, 554, 630, 734
SsiI CCGC 1 cut(s) 704
SspMI CTAG 3 cut(s) 96, 168, 392
StyD4I CCNGG 2 cut(s) 323, 713
TaaI ACNGT 3 cut(s) 137, 561, 690
TaiI ACGT 2 cut(s) 28, 238
TaqI TCGA 3 cut(s) 469, 635, 741
TasI AATT 7 cut(s) 182, 353, 481, 540, 554, 630, 734
TatI WGTACW 1 cut(s) 744
TfiI GAWTC 4 cut(s) 164, 386, 401, 680
Tru1I TTAA 1 cut(s) 778
Tru9I TTAA 1 cut(s) 778
TscAI CASTG 2 cut(s) 581, 693
TseI GCWGC 2 cut(s) 148, 338
TspDTI ATGAA 4 cut(s) 34, 347, 471, 672
TspGWI ACGGA 1 cut(s) 407
TspRI CASTG 2 cut(s) 581, 693
XapI RAATTY 2 cut(s) 353, 734
XmnI GAANNNNTTC 1 cut(s) 33
XspI CTAG 3 cut(s) 96, 168, 392
ZrmI AGTACT 1 cut(s) 746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.