AT3G13235

Aspartyl protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0007459
Physical Location & Seq
Reverse (-)
4271212 .. 4274487
3276 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G13235.1

Sequence Viewer

Length: 1245 bp
ATGAGGATCACTGTCATGACCGCCGGTGAACAAATCATCACTCTCGATGTCGATTCTCAGGAGACTGTTGAGAATGTTAAAGCCTTGCTCGAGGTTGAGTCCAATGTTCCGATTCAGCAGCAGCAGCTGTTGTATAATGGAAACGAGATGGGAAATTCCGATAAATTGAGTGCCTTGGGTGTTAAGGATGATGATTTATTGATGATGATGGTTTCCAATGCCTCATCTGGTAGTGCCACTAGTGCGGCGGGGAATGATTTGGGCATGAATCCTGATGGGTCAGCTTTGAACCCTGCAGCCTTTCAACAACACATTCGTGGGGATTCTAATCTTATGGGACAGTTATTTCAGAATGATCCTGAGCTTGCACAAGTGATTTCTGGAAGCGATTTAAATAAGTTACAGGATGTTTTGCGTGCACGGCATCGTCAGCGATCTGTTTTACAGCGTCAGAAGGAAGAGGAACTTGCCCTTCTGTATGCAGATCCTTTTGATGTTGAAGCACAGAGGAAAATTGAAGCTGCAATTCGTCAGAAAGGAATTGATGAGAACTGGGAAGCTGCCCTTGAACATAATCCTGAAGGTTTTGCTCGGGTGATAATGCTCTATGTGGATATGGAGGTCAACGGCGTACCTTTGAAGGCTTTCGTTGATAGTGGTGCACAGTCGACAATTATATCCAAGAGTTGTGCTGAGAGATGCGGATTGCTGAGACTTATGGACCAACGCTACAAAGGTATTGCCCATGGTGTTGGCCAAACAGAGATATTGGGCCGCATTCATGTTGCTCCTATCAAGATTGGAAACAACTTTTACCCTTGTTCATTCGTGGTGCTGGACTCACCTAACATGGAATTTCTGTTTGGCCTGGATATGCTGCGTAAGCATCAGTGCACTATAGATTTGAAGGAAAACGTCATGACTGTTGGAGGAGGAGAGGTCTCGGTTCCCTTTTTGCAAGAGAAAGACATCCCGTCCCGATTTCTGGATGAAGAGCGTGTTCCAAATGATGCATCTAGCTCTGGAGCAACGGTTCCTTCTGGGTTCACAGAGAAGAAGAATAACACTGTTGCAAACCCAACAAGCCAACAACCGAAAAGGCAGAACACATCAGAGGGACCTGAATTTGAAGCCAAGATTGCAAAGCTTGTGGAACTAGGGTTCTCCCGCGATTCAGTGATACAAGCTCTCAAATTGTTTGAAGGAAACGAAGAACAAGCTGCTGGGTTTCTCTTTGGCGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asp_protease UBA ubiquitin

Protein Analysis

414

Amino Acids

45.36

Weight (kDa)

4.83

Isoelectric Point (pI)

39.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 1 - 71 5.3e-08 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 3 - 72 4.1e-15 Ubiquitin family
Ddi2_HDD PF24669 97 - 164 3.8e-06 Ddi2 HDD domain
Asp_protease PF09668 193 - 301 2.7e-48 Aspartyl protease
gag-asp_proteas PF13975 204 - 295 3.6e-19 gag-polyprotein putative aspartyl protease
Asp_protease_2 PF13650 204 - 291 1.2e-11 Aspartyl protease
UBA PF00627 377 - 409 4.9e-07 UBA/TS-N domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 668
AccII CGCG 1 cut(s) 1170
AciI CCGC 7 cut(s) 21, 245, 248, 702, 775, 1168, 1239
AclWI GGATC 3 cut(s) 14, 350, 479
AcoI YGGCCR 1 cut(s) 754
AcsI RAATTY 3 cut(s) 154, 854, 1124
AcuI CTGAAG 1 cut(s) 600
AfaI GTAC 1 cut(s) 633
AfiI CCNNNNNNNGG 1 cut(s) 985
AgsI TTSAA 9 cut(s) 289, 305, 500, 518, 569, 640, 907, 1130, 1202
AhdI GACNNNNNGTC 1 cut(s) 973
AhlI ACTAGT 1 cut(s) 239
AjnI CCWGG 1 cut(s) 867
AjuI GAANNNNNNNTTGG 2 cut(s) 846, 878
AleI CACNNNNGTG 1 cut(s) 315
AloI GAACNNNNNNTCC 4 cut(s) 1017, 1049, 1145, 1177
AluBI AGCT 9 cut(s) 127, 284, 364, 521, 560, 1020, 1147, 1187, 1220
AluI AGCT 9 cut(s) 127, 284, 364, 521, 560, 1020, 1147, 1187, 1220
Alw21I GWGCWC 3 cut(s) 421, 664, 896
Alw26I GTCTC 3 cut(s) 56, 706, 946
Alw44I GTGCAC 3 cut(s) 417, 660, 892
AlwI GGATC 3 cut(s) 14, 350, 479
AlwNI CAGNNNCTG 1 cut(s) 127
Ama87I CYCGRG 2 cut(s) 89, 591
AoxI GGCC 3 cut(s) 754, 772, 865
ApaLI GTGCAC 3 cut(s) 417, 660, 892
ApeKI GCWGC 8 cut(s) 118, 121, 124, 296, 521, 560, 877, 1220
ApoI RAATTY 3 cut(s) 154, 854, 1124
Asp700I GAANNNNTTC 1 cut(s) 644
AspS9I GGNCC 3 cut(s) 721, 772, 1118
AsuHPI GGTGA 3 cut(s) 38, 607, 834
AvaI CYCGRG 2 cut(s) 89, 591
AvaII GGWCC 2 cut(s) 721, 1118
BaeGI GKGCMC 3 cut(s) 421, 664, 896
BalI TGGCCA 1 cut(s) 756
Bbv12I GWGCWC 3 cut(s) 421, 664, 896
BbvI GCAGC 8 cut(s) 130, 133, 136, 308, 508, 547, 864, 1207
BccI CCATC 3 cut(s) 142, 202, 269
BceAI ACGGC 2 cut(s) 437, 643
BciT130I CCWGG 1 cut(s) 869
BcoDI GTCTC 3 cut(s) 56, 706, 946
BcuI ACTAGT 1 cut(s) 239
BfaI CTAG 3 cut(s) 240, 1017, 1157
BfmI CTRYAG 2 cut(s) 294, 897
Bme1390I CCNGG 1 cut(s) 869
Bme18I GGWCC 2 cut(s) 721, 1118
BmeRI GACNNNNNGTC 1 cut(s) 973
BmeT110I CYCGRG 2 cut(s) 89, 591
BmgT120I GGNCC 3 cut(s) 721, 772, 1118
BmiI GGNNCC 3 cut(s) 948, 1035, 1119
BmrFI CCNGG 1 cut(s) 869
BmrI ACTGGG 1 cut(s) 562
BmsI GCATC 5 cut(s) 433, 689, 895, 1000, 1022
BmuI ACTGGG 1 cut(s) 562
BpmI CTGGAG 1 cut(s) 1044
Bpu10I CCTNAGC 1 cut(s) 360
BsaBI GATNNNNATC 1 cut(s) 327
BsaI GGTCTC 1 cut(s) 946
BsaJI CCNNGG 2 cut(s) 174, 745
BsaXI ACNNNNNCTCC 4 cut(s) 924, 954, 1017, 1047
Bsc4I CCNNNNNNNGG 1 cut(s) 985
Bse118I RCCGGY 1 cut(s) 23
Bse1I ACTGG 1 cut(s) 557
Bse8I GATNNNNATC 1 cut(s) 327
BseBI CCWGG 1 cut(s) 869
BseDI CCNNGG 2 cut(s) 174, 745
BseGI GGATG 4 cut(s) 193, 412, 969, 994
BseJI GATNNNNATC 1 cut(s) 327
BseLI CCNNNNNNNGG 1 cut(s) 985
BseMII CTCAG 4 cut(s) 71, 351, 684, 701
BseNI ACTGG 1 cut(s) 557
BseRI GAGGAG 2 cut(s) 945, 948
BseSI GKGCMC 3 cut(s) 421, 664, 896
BseXI GCAGC 8 cut(s) 130, 133, 136, 308, 508, 547, 864, 1207
BseYI CCCAGC 1 cut(s) 1223
Bsh1236I CGCG 1 cut(s) 1170
BshFI GGCC 3 cut(s) 756, 774, 867
BsiHKAI GWGCWC 3 cut(s) 421, 664, 896
BsiHKCI CYCGRG 2 cut(s) 89, 591
BsiSI CCGG 1 cut(s) 24
BslFI GGGAC 3 cut(s) 351, 961, 1131
BslI CCNNNNNNNGG 1 cut(s) 985
BsmAI GTCTC 3 cut(s) 56, 706, 946
BsmFI GGGAC 3 cut(s) 351, 961, 1131
BsmI GAATGC 1 cut(s) 777
BsnI GGCC 3 cut(s) 756, 774, 867
Bso31I GGTCTC 1 cut(s) 946
BsoBI CYCGRG 2 cut(s) 89, 591
Bsp1286I GDGCHC 3 cut(s) 421, 664, 896
Bsp143I GATC 4 cut(s) 6, 355, 434, 484
Bsp19I CCATGG 1 cut(s) 745
BspACI CCGC 7 cut(s) 21, 245, 248, 702, 775, 1168, 1239
BspANI GGCC 3 cut(s) 756, 774, 867
BspCNI CTCAG 4 cut(s) 70, 352, 685, 702
BspFNI CGCG 1 cut(s) 1170
BspHI TCATGA 2 cut(s) 15, 918
BspLI GGNNCC 3 cut(s) 948, 1035, 1119
BspMAI CTGCAG 1 cut(s) 298
BspPI GGATC 3 cut(s) 14, 350, 479
BspQI GCTCTTC 1 cut(s) 987
BspTNI GGTCTC 1 cut(s) 946
BsrFI RCCGGY 1 cut(s) 23
BsrI ACTGG 1 cut(s) 557
BssAI RCCGGY 1 cut(s) 23
BssECI CCNNGG 2 cut(s) 174, 745
BssMI GATC 4 cut(s) 6, 355, 434, 484
BssT1I CCWWGG 2 cut(s) 174, 745
Bst2UI CCWGG 1 cut(s) 869
Bst4CI ACNGT 7 cut(s) 13, 67, 342, 666, 925, 1033, 1069
Bst6I CTCTTC 2 cut(s) 453, 987
BstC8I GCNNGC 2 cut(s) 366, 417
BstDEI CTNAG 4 cut(s) 57, 360, 693, 710
BstDSI CCRYGG 1 cut(s) 745
BstF5I GGATG 4 cut(s) 193, 412, 969, 994
BstFNI CGCG 1 cut(s) 1170
BstKTI GATC 4 cut(s) 9, 358, 437, 487
BstMAI GTCTC 3 cut(s) 56, 706, 946
BstMBI GATC 4 cut(s) 6, 355, 434, 484
BstMWI GCNNNNNNNGC 6 cut(s) 124, 242, 421, 430, 883, 1139
BstNI CCWGG 1 cut(s) 869
BstSCI CCNGG 1 cut(s) 867
BstSFI CTRYAG 2 cut(s) 294, 897
BstSLI GKGCMC 3 cut(s) 421, 664, 896
BstUI CGCG 1 cut(s) 1170
BstV1I GCAGC 8 cut(s) 130, 133, 136, 308, 508, 547, 864, 1207
BstX2I RGATCY 1 cut(s) 484
BstXI CCANNNNNNTGG 1 cut(s) 752
BstYI RGATCY 1 cut(s) 484
BsuRI GGCC 3 cut(s) 756, 774, 867
BtgI CCRYGG 1 cut(s) 745
BtsCI GGATG 4 cut(s) 193, 412, 969, 994
BtsIMutI CAGTG 4 cut(s) 9, 896, 1065, 1182
Cac8I GCNNGC 2 cut(s) 366, 417
CaiI CAGNNNCTG 1 cut(s) 127
CciI TCATGA 2 cut(s) 15, 918
Cfr10I RCCGGY 1 cut(s) 23
Cfr13I GGNCC 3 cut(s) 721, 772, 1118
CseI GACGC 1 cut(s) 437
Csp6I GTAC 1 cut(s) 632
CspCI CAANNNNNGTGG 2 cut(s) 1131, 1166
CviAII CATG 6 cut(s) 16, 265, 746, 782, 850, 919
CviQI GTAC 1 cut(s) 632
DdeI CTNAG 4 cut(s) 57, 360, 693, 710
DpnI GATC 4 cut(s) 8, 357, 436, 486
DpnII GATC 4 cut(s) 6, 355, 434, 484
DraI TTTAAA 1 cut(s) 393
DriI GACNNNNNGTC 1 cut(s) 973
EaeI YGGCCR 1 cut(s) 754
Eam1104I CTCTTC 2 cut(s) 453, 987
Eam1105I GACNNNNNGTC 1 cut(s) 973
EarI CTCTTC 2 cut(s) 453, 987
Eco130I CCWWGG 2 cut(s) 174, 745
Eco31I GGTCTC 1 cut(s) 946
Eco47I GGWCC 2 cut(s) 721, 1118
Eco57I CTGAAG 1 cut(s) 600
Eco88I CYCGRG 2 cut(s) 89, 591
EcoO109I RGGNCCY 1 cut(s) 1118
EcoRII CCWGG 1 cut(s) 867
EcoT14I CCWWGG 2 cut(s) 174, 745
EcoT22I ATGCAT 1 cut(s) 1015
ErhI CCWWGG 2 cut(s) 174, 745
FaeI CATG 6 cut(s) 19, 268, 749, 785, 853, 922
FalI AAGNNNNNCTT 4 cut(s) 450, 482, 549, 581
FaqI GGGAC 3 cut(s) 351, 961, 1131
FatI CATG 6 cut(s) 15, 264, 745, 781, 849, 918
FauI CCCGC 2 cut(s) 241, 1175
FblI GTMKAC 1 cut(s) 668
FokI GGATG 4 cut(s) 200, 419, 956, 1001
FspBI CTAG 3 cut(s) 240, 1017, 1157
GsaI CCCAGC 1 cut(s) 1227
GsuI CTGGAG 1 cut(s) 1044
HaeIII GGCC 3 cut(s) 756, 774, 867
HapII CCGG 1 cut(s) 24
HgaI GACGC 1 cut(s) 437
Hin1II CATG 6 cut(s) 19, 268, 749, 785, 853, 922
HincII GTYRAC 2 cut(s) 625, 669
HindII GTYRAC 2 cut(s) 625, 669
HindIII AAGCTT 1 cut(s) 1145
HinfI GANTC 7 cut(s) 53, 98, 112, 268, 323, 839, 1172
HpaII CCGG 1 cut(s) 24
HphI GGTGA 3 cut(s) 38, 607, 834
Hpy166II GTNNAC 7 cut(s) 29, 419, 625, 662, 669, 894, 1047
Hpy188I TCNGA 6 cut(s) 111, 160, 351, 453, 534, 1114
Hpy8I GTNNAC 7 cut(s) 29, 419, 625, 662, 669, 894, 1047
HpyAV CCTTC 7 cut(s) 448, 482, 575, 634, 901, 1047, 1196
HpyCH4III ACNGT 7 cut(s) 13, 67, 342, 666, 925, 1033, 1069
HpyCH4IV ACGT 1 cut(s) 915
HpyF10VI GCNNNNNNNGC 6 cut(s) 124, 242, 421, 430, 883, 1139
HpyF3I CTNAG 4 cut(s) 57, 360, 693, 710
HpySE526I ACGT 1 cut(s) 915
Hsp92II CATG 6 cut(s) 19, 268, 749, 785, 853, 922
Kzo9I GATC 4 cut(s) 6, 355, 434, 484
LguI GCTCTTC 1 cut(s) 987
LmnI GCTCC 2 cut(s) 793, 1025
Lsp1109I GCAGC 8 cut(s) 130, 133, 136, 308, 508, 547, 864, 1207
LweI GCATC 5 cut(s) 433, 689, 895, 1000, 1022
MaeI CTAG 3 cut(s) 240, 1017, 1157
MaeII ACGT 1 cut(s) 915
MaeIII GTNAC 1 cut(s) 399
MalI GATC 4 cut(s) 8, 357, 436, 486
MboI GATC 4 cut(s) 6, 355, 434, 484
MboII GAAGA 5 cut(s) 470, 1004, 1066, 1069, 1223
MflI RGATCY 1 cut(s) 484
MhlI GDGCHC 3 cut(s) 421, 664, 896
MlsI TGGCCA 1 cut(s) 756
MluCI AATT 9 cut(s) 154, 164, 513, 525, 540, 672, 854, 1124, 1193
MluNI TGGCCA 1 cut(s) 756
MlyI GAGTC 2 cut(s) 107, 833
MmeI TCCRAC 1 cut(s) 907
MnlI CCTC 9 cut(s) 85, 232, 454, 501, 613, 923, 926, 931, 1108
Mox20I TGGCCA 1 cut(s) 756
Mph1103I ATGCAT 1 cut(s) 1015
MroXI GAANNNNTTC 1 cut(s) 644
MscI TGGCCA 1 cut(s) 756
MseI TTAA 3 cut(s) 78, 183, 392
MslI CAYNNNNRTG 2 cut(s) 14, 315
Msp20I TGGCCA 1 cut(s) 756
MspA1I CMGCKG 1 cut(s) 127
MspI CCGG 1 cut(s) 24
MspR9I CCNGG 1 cut(s) 869
Mva1269I GAATGC 1 cut(s) 777
MvaI CCWGG 1 cut(s) 869
MvnI CGCG 1 cut(s) 1170
MwoI GCNNNNNNNGC 6 cut(s) 124, 242, 421, 430, 883, 1139
NcoI CCATGG 1 cut(s) 745
NdeII GATC 4 cut(s) 6, 355, 434, 484
NlaIII CATG 6 cut(s) 19, 268, 749, 785, 853, 922
NlaIV GGNNCC 3 cut(s) 948, 1035, 1119
NsiI ATGCAT 1 cut(s) 1015
OliI CACNNNNGTG 1 cut(s) 315
PaeR7I CTCGAG 1 cut(s) 89
PagI TCATGA 2 cut(s) 15, 918
PciSI GCTCTTC 1 cut(s) 987
PctI GAATGC 1 cut(s) 777
PdmI GAANNNNTTC 1 cut(s) 644
PfeI GAWTC 5 cut(s) 53, 112, 268, 323, 1172
PleI GAGTC 2 cut(s) 106, 833
PpsI GAGTC 2 cut(s) 106, 833
PpuMI RGGWCCY 1 cut(s) 1118
Psp5II RGGWCCY 1 cut(s) 1118
Psp6I CCWGG 1 cut(s) 867
PspFI CCCAGC 1 cut(s) 1223
PspGI CCWGG 1 cut(s) 867
PspN4I GGNNCC 3 cut(s) 948, 1035, 1119
PspPI GGNCC 3 cut(s) 721, 772, 1118
PspPPI RGGWCCY 1 cut(s) 1118
PspXI VCTCGAGB 1 cut(s) 89
PstI CTGCAG 1 cut(s) 298
PstNI CAGNNNCTG 1 cut(s) 127
PsuI RGATCY 1 cut(s) 484
PvuII CAGCTG 1 cut(s) 127
RsaI GTAC 1 cut(s) 633
RsaNI GTAC 1 cut(s) 632
RseI CAYNNNNRTG 2 cut(s) 14, 315
SalI GTCGAC 1 cut(s) 667
SapI GCTCTTC 1 cut(s) 987
SaqAI TTAA 3 cut(s) 78, 183, 392
Sau3AI GATC 4 cut(s) 6, 355, 434, 484
Sau96I GGNCC 3 cut(s) 721, 772, 1118
SchI GAGTC 2 cut(s) 107, 833
ScrFI CCNGG 1 cut(s) 869
SduI GDGCHC 3 cut(s) 421, 664, 896
SfaNI GCATC 5 cut(s) 433, 689, 895, 1000, 1022
SfcI CTRYAG 2 cut(s) 294, 897
Sfr274I CTCGAG 1 cut(s) 89
SgrAI CRCCGGYG 1 cut(s) 23
SinI GGWCC 2 cut(s) 721, 1118
SlaI CTCGAG 1 cut(s) 89
SmiI ATTTAAAT 1 cut(s) 393
SmiMI CAYNNNNRTG 2 cut(s) 14, 315
SmlI CTYRAG 1 cut(s) 89
SmoI CTYRAG 1 cut(s) 89
SpeI ACTAGT 1 cut(s) 239
Sse9I AATT 9 cut(s) 154, 164, 513, 525, 540, 672, 854, 1124, 1193
SsiI CCGC 7 cut(s) 21, 245, 248, 702, 775, 1168, 1239
SspMI CTAG 3 cut(s) 240, 1017, 1157
StyD4I CCNGG 1 cut(s) 867
StyI CCWWGG 2 cut(s) 174, 745
SwaI ATTTAAAT 1 cut(s) 393
TaaI ACNGT 7 cut(s) 13, 67, 342, 666, 925, 1033, 1069
TaiI ACGT 1 cut(s) 918
TaqI TCGA 4 cut(s) 45, 51, 90, 668
TasI AATT 9 cut(s) 154, 164, 513, 525, 540, 672, 854, 1124, 1193
TauI GCSGC 3 cut(s) 248, 777, 1242
TfiI GAWTC 5 cut(s) 53, 112, 268, 323, 1172
Tru1I TTAA 3 cut(s) 78, 183, 392
Tru9I TTAA 3 cut(s) 78, 183, 392
TscAI CASTG 4 cut(s) 16, 896, 1072, 1182
TseI GCWGC 8 cut(s) 118, 121, 124, 296, 521, 560, 877, 1220
TspDTI ATGAA 4 cut(s) 281, 770, 813, 1005
TspRI CASTG 4 cut(s) 16, 896, 1072, 1182
VneI GTGCAC 3 cut(s) 417, 660, 892
VpaK11BI GGWCC 2 cut(s) 721, 1118
XapI RAATTY 3 cut(s) 154, 854, 1124
XhoI CTCGAG 1 cut(s) 89
XmiI GTMKAC 1 cut(s) 668
XmnI GAANNNNTTC 1 cut(s) 644
XspI CTAG 3 cut(s) 240, 1017, 1157
Zsp2I ATGCAT 1 cut(s) 1015
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.