Rw5G045870

DNA damage-inducible protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0007459
Physical Location & Seq
Forward (+)
79881733 .. 79886460
4728 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G045870.1

Sequence Viewer

Length: 1230 bp
ATGAAGATCACAGTAATGACCGCCGACGAGCAGATCATCAGCTTAGACGTCGATCCTCATGAGACTGTTGAAAACGTGAAAGCTCTGCTGGAGGTGGAGACTCGGGTGCTGCTTCAGAATCAGCAGCTGCTGTATAATGGGAAGGAGGTTAGAAATTCCGAGAGACTGAGTGCGCTCGGTGTCAAAGATGATGATTTAATAATGATGGTATCCAATGCCGTGTCTAGTGCTTCCAGCAATGCACTGAGCTTCAATCCAGATGGGTCTGCTGCAAATCCACTAGCTTTCCAGCAGCAGATTAGGAATGATTCTAATGTGATAGCACAGTTGTTTCAGAGTGATCCTGAATTAGCACAAGTTATTCTTGGAGACGATCTCAATAGACTGCAGGAAGTTTTACGAGAGCGTTATCGCCAAAAATCTGAACATCGACGACAACAAGAGGAGGAGAATGCTCTTCTTTTTGCAGATCCTTTTGATGTTGAAGCCCAGAAGAAGATTGAAGCTGCTATTCGCCAGAAAGGAATTGATGAAAATTGGGCTGCTGCTCTTGAACATAACCCCGAAGCTTTTGCAAGGGTGGTTATGCTGTATGTAGACATGGAAGTTAATGGTGTCCCATTGAAGGCATTTGTTGATAGTGGAGCACAGTCAACAATTATATCAAAAAGCTGTGCTGAGCGTTGTGGATTGTTGAGGCTTTTAGATCAACGTTACAAGGGCATTGCTCATGGAGTTGGTCAATCAGAGATATTAGGTCGAATACACGTAGCTCCTATCAAGATTGGAAATAATTTTTACCCCTGCTCCTTCCTGGTACTGGATGCTACTAATATAGAGTTCCTCTTTGGGTTGGATATGCTTCGAAAGCATCAGTGCAGTATAGACTTGAAGGATAATGTGTTGAGAGTTGGTGGGGGAGAAGTCTCTGTCCCATTTTTACAAGAAAAAGATTTGCCATCTCGGTTTCTGGATGAAGGAAAGTTGTTCCAGGAAGCATCCAGCTCAGGAGCTCCTGTGATAGCTGGAACAACAGATAAGACCAAAAGTGTATCAACTGGAGGGCAATCATCTGGAAGTGCTCGTCCTGATACAAGTCAGGGACCTGATTTCGAAGCTAAAGTTGCAAAGCTAGTTGAGCTAGGGTTTGGGAGAGATGCAGTAATACAAGCTCTTAAATTTTTTGATGGCAATGAAGAACAAGCCGCAGGGTTTCTCTTTGGGGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asp_protease UBA ubiquitin

Protein Analysis

409

Amino Acids

44.85

Weight (kDa)

4.91

Isoelectric Point (pI)

36.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 1 - 69 1.5e-06 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 3 - 70 3.5e-13 Ubiquitin family
Ddi2_HDD PF24669 92 - 159 4.2e-06 Ddi2 HDD domain
Asp_protease PF09668 188 - 296 9.5e-49 Aspartyl protease
gag-asp_proteas PF13975 199 - 290 7.7e-18 gag-polyprotein putative aspartyl protease
Asp_protease_2 PF13650 199 - 286 6.5e-12 Aspartyl protease
UBA PF00627 372 - 404 2.7e-08 UBA/TS-N domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 51
AccI GTMKAC 1 cut(s) 597
AciI CCGC 2 cut(s) 21, 1206
AclI AACGTT 1 cut(s) 712
AclWI GGATC 3 cut(s) 47, 335, 464
AcsI RAATTY 2 cut(s) 154, 1178
AcuI CTGAAG 1 cut(s) 98
AcyI GRCGYC 1 cut(s) 48
AfaI GTAC 1 cut(s) 819
AfiI CCNNNNNNNGG 2 cut(s) 625, 820
AflIII ACRYGT 1 cut(s) 766
AgsI TTSAA 7 cut(s) 71, 253, 485, 503, 554, 625, 892
AjnI CCWGG 2 cut(s) 813, 990
Alw21I GWGCWC 3 cut(s) 649, 1015, 1084
Alw26I GTCTC 5 cut(s) 56, 92, 157, 363, 931
AlwI GGATC 3 cut(s) 47, 335, 464
AlwNI CAGNNNCTG 2 cut(s) 127, 130
Ama87I CYCGRG 1 cut(s) 102
ApeKI GCWGC 8 cut(s) 109, 124, 127, 269, 292, 506, 542, 545
ApoI RAATTY 2 cut(s) 154, 1178
AspLEI GCGC 1 cut(s) 175
AspS9I GGNCC 1 cut(s) 1103
AsuII TTCGAA 2 cut(s) 865, 1113
AvaI CYCGRG 1 cut(s) 102
AvaII GGWCC 1 cut(s) 1103
BanII GRGCYC 1 cut(s) 1015
Bbv12I GWGCWC 3 cut(s) 649, 1015, 1084
BbvI GCAGC 8 cut(s) 96, 114, 136, 256, 304, 493, 529, 532
BccI CCATC 4 cut(s) 199, 254, 967, 1181
BceAI ACGGC 1 cut(s) 203
BcgI CGANNNNNNTGC 2 cut(s) 554, 588
BciT130I CCWGG 2 cut(s) 815, 992
BciVI GTATCC 1 cut(s) 220
BcoDI GTCTC 5 cut(s) 56, 92, 157, 363, 931
BfaI CTAG 4 cut(s) 225, 281, 1133, 1142
BfmI CTRYAG 1 cut(s) 386
BfuI GTATCC 1 cut(s) 220
BisI GCNGC 9 cut(s) 110, 125, 128, 270, 293, 507, 543, 546, 1206
BlpI GCTNAGC 1 cut(s) 678
BlsI GCNGC 9 cut(s) 111, 126, 129, 271, 294, 508, 544, 547, 1207
Bme1390I CCNGG 2 cut(s) 815, 992
Bme18I GGWCC 1 cut(s) 1103
BmeT110I CYCGRG 1 cut(s) 102
BmgT120I GGNCC 1 cut(s) 1103
BmiI GGNNCC 1 cut(s) 1104
BmrFI CCNGG 2 cut(s) 815, 992
BmsI GCATC 4 cut(s) 814, 880, 1007, 1147
BplI GAGNNNNNCTC 2 cut(s) 360, 392
BpmI CTGGAG 2 cut(s) 110, 1080
Bpu10I CCTNAGC 1 cut(s) 1006
Bpu1102I GCTNAGC 1 cut(s) 678
Bpu14I TTCGAA 2 cut(s) 865, 1113
BsaAI YACGTR 1 cut(s) 769
BsaHI GRCGYC 1 cut(s) 48
BsaXI ACNNNNNCTCC 4 cut(s) 791, 821, 1002, 1032
Bsc4I CCNNNNNNNGG 2 cut(s) 625, 820
Bse1I ACTGG 2 cut(s) 825, 1063
Bse3DI GCAATG 3 cut(s) 244, 723, 1198
BseBI CCWGG 2 cut(s) 815, 992
BseGI GGATG 3 cut(s) 829, 979, 998
BseLI CCNNNNNNNGG 2 cut(s) 625, 820
BseMI GCAATG 3 cut(s) 244, 723, 1198
BseMII CTCAG 4 cut(s) 158, 236, 669, 1020
BseNI ACTGG 2 cut(s) 825, 1063
BseRI GAGGAG 2 cut(s) 458, 461
BseXI GCAGC 8 cut(s) 96, 114, 136, 256, 304, 493, 529, 532
BsgI GTGCAG 1 cut(s) 898
BsiHKAI GWGCWC 3 cut(s) 649, 1015, 1084
BsiHKCI CYCGRG 1 cut(s) 102
BslFI GGGAC 3 cut(s) 602, 917, 1116
BslI CCNNNNNNNGG 2 cut(s) 625, 820
BsmAI GTCTC 5 cut(s) 56, 92, 157, 363, 931
BsmBI CGTCTC 1 cut(s) 363
BsmFI GGGAC 3 cut(s) 602, 917, 1116
BsmI GAATGC 1 cut(s) 457
BsoBI CYCGRG 1 cut(s) 102
Bsp119I TTCGAA 2 cut(s) 865, 1113
Bsp1286I GDGCHC 3 cut(s) 649, 1015, 1084
Bsp143I GATC 7 cut(s) 6, 33, 52, 340, 373, 469, 706
Bsp1720I GCTNAGC 1 cut(s) 678
BspACI CCGC 2 cut(s) 21, 1206
BspCNI CTCAG 4 cut(s) 159, 237, 670, 1019
BspHI TCATGA 1 cut(s) 58
BspLI GGNNCC 1 cut(s) 1104
BspMAI CTGCAG 1 cut(s) 390
BspPI GGATC 3 cut(s) 47, 335, 464
BspQI GCTCTTC 1 cut(s) 462
BspT104I TTCGAA 2 cut(s) 865, 1113
BsrDI GCAATG 3 cut(s) 244, 723, 1198
BsrI ACTGG 2 cut(s) 825, 1063
BssMI GATC 7 cut(s) 6, 33, 52, 340, 373, 469, 706
BssNI GRCGYC 1 cut(s) 48
Bst2UI CCWGG 2 cut(s) 815, 992
Bst4CI ACNGT 4 cut(s) 13, 67, 327, 651
Bst6I CTCTTC 1 cut(s) 462
BstACI GRCGYC 1 cut(s) 48
BstBAI YACGTR 1 cut(s) 769
BstBI TTCGAA 2 cut(s) 865, 1113
BstDEI CTNAG 5 cut(s) 43, 167, 245, 678, 1006
BstF5I GGATG 3 cut(s) 829, 979, 998
BstHHI GCGC 1 cut(s) 175
BstKTI GATC 7 cut(s) 9, 36, 55, 343, 376, 472, 709
BstMAI GTCTC 5 cut(s) 56, 92, 157, 363, 931
BstMBI GATC 7 cut(s) 6, 33, 52, 340, 373, 469, 706
BstMWI GCNNNNNNNGC 3 cut(s) 868, 1124, 1138
BstNI CCWGG 2 cut(s) 815, 992
BstSCI CCNGG 2 cut(s) 813, 990
BstSFI CTRYAG 1 cut(s) 386
BstV1I GCAGC 8 cut(s) 96, 114, 136, 256, 304, 493, 529, 532
BstX2I RGATCY 1 cut(s) 469
BstYI RGATCY 1 cut(s) 469
BsuI GTATCC 1 cut(s) 220
BtsCI GGATG 3 cut(s) 829, 979, 998
BtsIMutI CAGTG 2 cut(s) 242, 881
CaiI CAGNNNCTG 2 cut(s) 127, 130
CciI TCATGA 1 cut(s) 58
CfoI GCGC 1 cut(s) 175
Cfr13I GGNCC 1 cut(s) 1103
Csp6I GTAC 1 cut(s) 818
CviAII CATG 3 cut(s) 59, 601, 731
CviQI GTAC 1 cut(s) 818
DdeI CTNAG 5 cut(s) 43, 167, 245, 678, 1006
DpnI GATC 7 cut(s) 8, 35, 54, 342, 375, 471, 708
DpnII GATC 7 cut(s) 6, 33, 52, 340, 373, 469, 706
Eam1104I CTCTTC 1 cut(s) 462
EarI CTCTTC 1 cut(s) 462
Ecl136II GAGCTC 1 cut(s) 1013
Eco24I GRGCYC 1 cut(s) 1015
Eco47I GGWCC 1 cut(s) 1103
Eco53kI GAGCTC 1 cut(s) 1013
Eco57I CTGAAG 1 cut(s) 98
Eco88I CYCGRG 1 cut(s) 102
EcoICRI GAGCTC 1 cut(s) 1013
EcoO109I RGGNCCY 1 cut(s) 1103
EcoRII CCWGG 2 cut(s) 813, 990
EcoT38I GRGCYC 1 cut(s) 1015
Esp3I CGTCTC 1 cut(s) 363
FaeI CATG 3 cut(s) 62, 604, 734
FalI AAGNNNNNCTT 2 cut(s) 348, 380
FaqI GGGAC 3 cut(s) 602, 917, 1116
FatI CATG 3 cut(s) 58, 600, 730
FblI GTMKAC 1 cut(s) 597
Fnu4HI GCNGC 9 cut(s) 110, 125, 128, 270, 293, 507, 543, 546, 1206
FokI GGATG 3 cut(s) 836, 985, 986
FriOI GRGCYC 1 cut(s) 1015
Fsp4HI GCNGC 9 cut(s) 110, 125, 128, 270, 293, 507, 543, 546, 1206
FspBI CTAG 4 cut(s) 225, 281, 1133, 1142
GlaI GCGC 1 cut(s) 174
GluI GCNGC 9 cut(s) 110, 125, 128, 270, 293, 507, 543, 546, 1206
GsuI CTGGAG 2 cut(s) 110, 1080
HhaI GCGC 1 cut(s) 175
Hin1I GRCGYC 1 cut(s) 48
Hin1II CATG 3 cut(s) 62, 604, 734
Hin6I GCGC 1 cut(s) 173
HinP1I GCGC 1 cut(s) 173
HincII GTYRAC 1 cut(s) 654
HindII GTYRAC 1 cut(s) 654
HindIII AAGCTT 1 cut(s) 567
HinfI GANTC 3 cut(s) 100, 118, 308
Hpy166II GTNNAC 2 cut(s) 598, 654
Hpy188I TCNGA 5 cut(s) 117, 160, 336, 424, 748
Hpy188III TCNNGA 9 cut(s) 59, 257, 344, 551, 781, 971, 1008, 1074, 1088
Hpy8I GTNNAC 2 cut(s) 598, 654
Hpy99I CGWCG 3 cut(s) 29, 53, 435
HpyAV CCTTC 5 cut(s) 136, 619, 820, 886, 971
HpyCH4III ACNGT 4 cut(s) 13, 67, 327, 651
HpyCH4IV ACGT 4 cut(s) 48, 75, 712, 768
HpyCH4V TGCA 8 cut(s) 242, 272, 388, 467, 575, 879, 1127, 1160
HpyF10VI GCNNNNNNNGC 3 cut(s) 868, 1124, 1138
HpyF3I CTNAG 5 cut(s) 43, 167, 245, 678, 1006
HpySE526I ACGT 4 cut(s) 48, 75, 712, 768
Hsp92I GRCGYC 1 cut(s) 48
Hsp92II CATG 3 cut(s) 62, 604, 734
HspAI GCGC 1 cut(s) 173
Kzo9I GATC 7 cut(s) 6, 33, 52, 340, 373, 469, 706
LguI GCTCTTC 1 cut(s) 462
LmnI GCTCC 5 cut(s) 644, 778, 812, 1010, 1018
Lsp1109I GCAGC 8 cut(s) 96, 114, 136, 256, 304, 493, 529, 532
LweI GCATC 4 cut(s) 814, 880, 1007, 1147
MaeI CTAG 4 cut(s) 225, 281, 1133, 1142
MaeII ACGT 4 cut(s) 48, 75, 712, 768
MaeIII GTNAC 1 cut(s) 713
MalI GATC 7 cut(s) 8, 35, 54, 342, 375, 471, 708
MboI GATC 7 cut(s) 6, 33, 52, 340, 373, 469, 706
MboII GAAGA 5 cut(s) 16, 449, 505, 508, 1208
MflI RGATCY 1 cut(s) 469
MhlI GDGCHC 3 cut(s) 649, 1015, 1084
MluCI AATT 7 cut(s) 154, 347, 525, 535, 657, 793, 1178
MlyI GAGTC 1 cut(s) 94
MmeI TCCRAC 1 cut(s) 834
MnlI CCTC 8 cut(s) 66, 85, 139, 436, 439, 690, 854, 1055
MseI TTAA 3 cut(s) 197, 609, 1176
MslI CAYNNNNRTG 1 cut(s) 14
MspA1I CMGCKG 1 cut(s) 127
MspR9I CCNGG 2 cut(s) 815, 992
Mva1269I GAATGC 1 cut(s) 457
MvaI CCWGG 2 cut(s) 815, 992
MwoI GCNNNNNNNGC 3 cut(s) 868, 1124, 1138
NdeII GATC 7 cut(s) 6, 33, 52, 340, 373, 469, 706
NlaIII CATG 3 cut(s) 62, 604, 734
NlaIV GGNNCC 1 cut(s) 1104
NspV TTCGAA 2 cut(s) 865, 1113
PagI TCATGA 1 cut(s) 58
PciSI GCTCTTC 1 cut(s) 462
PctI GAATGC 1 cut(s) 457
PfeI GAWTC 2 cut(s) 118, 308
PfoI TCCNGGA 1 cut(s) 990
PkrI GCNGC 9 cut(s) 111, 126, 129, 271, 294, 508, 544, 547, 1207
PleI GAGTC 1 cut(s) 94
PpsI GAGTC 1 cut(s) 94
Ppu21I YACGTR 1 cut(s) 769
PpuMI RGGWCCY 1 cut(s) 1103
Psp124BI GAGCTC 1 cut(s) 1015
Psp1406I AACGTT 1 cut(s) 712
Psp5II RGGWCCY 1 cut(s) 1103
Psp6I CCWGG 2 cut(s) 813, 990
PspGI CCWGG 2 cut(s) 813, 990
PspN4I GGNNCC 1 cut(s) 1104
PspPI GGNCC 1 cut(s) 1103
PspPPI RGGWCCY 1 cut(s) 1103
PstI CTGCAG 1 cut(s) 390
PstNI CAGNNNCTG 2 cut(s) 127, 130
PsuI RGATCY 1 cut(s) 469
PvuII CAGCTG 1 cut(s) 127
RsaI GTAC 1 cut(s) 819
RsaNI GTAC 1 cut(s) 818
RseI CAYNNNNRTG 1 cut(s) 14
SacI GAGCTC 1 cut(s) 1015
SapI GCTCTTC 1 cut(s) 462
SaqAI TTAA 3 cut(s) 197, 609, 1176
SatI GCNGC 9 cut(s) 110, 125, 128, 270, 293, 507, 543, 546, 1206
Sau3AI GATC 7 cut(s) 6, 33, 52, 340, 373, 469, 706
Sau96I GGNCC 1 cut(s) 1103
SchI GAGTC 1 cut(s) 94
ScrFI CCNGG 2 cut(s) 815, 992
SduI GDGCHC 3 cut(s) 649, 1015, 1084
SfaNI GCATC 4 cut(s) 814, 880, 1007, 1147
SfcI CTRYAG 1 cut(s) 386
SfuI TTCGAA 2 cut(s) 865, 1113
SinI GGWCC 1 cut(s) 1103
SmiMI CAYNNNNRTG 1 cut(s) 14
Sse9I AATT 7 cut(s) 154, 347, 525, 535, 657, 793, 1178
SsiI CCGC 2 cut(s) 21, 1206
SspMI CTAG 4 cut(s) 225, 281, 1133, 1142
SstI GAGCTC 1 cut(s) 1015
StyD4I CCNGG 2 cut(s) 813, 990
TaaI ACNGT 4 cut(s) 13, 67, 327, 651
TaiI ACGT 4 cut(s) 51, 78, 715, 771
TaqI TCGA 5 cut(s) 51, 430, 760, 865, 1113
TasI AATT 7 cut(s) 154, 347, 525, 535, 657, 793, 1178
TauI GCSGC 1 cut(s) 1208
TfiI GAWTC 2 cut(s) 118, 308
Tru1I TTAA 3 cut(s) 197, 609, 1176
Tru9I TTAA 3 cut(s) 197, 609, 1176
TscAI CASTG 2 cut(s) 249, 881
TseI GCWGC 8 cut(s) 109, 124, 127, 269, 292, 506, 542, 545
TspDTI ATGAA 4 cut(s) 17, 546, 990, 1209
TspRI CASTG 2 cut(s) 249, 881
VpaK11BI GGWCC 1 cut(s) 1103
XapI RAATTY 2 cut(s) 154, 1178
XmiI GTMKAC 1 cut(s) 597
XspI CTAG 4 cut(s) 225, 281, 1133, 1142
ZraI GACGTC 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.