MD03G1039600.v1.1

DNA damage-inducible protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0007459
Physical Location & Seq
Reverse (-)
3139130 .. 3144218
5089 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1039600.v1.1.491

Sequence Viewer

Length: 1230 bp
ATGAAGATCACTGTGATGACCGCGGACGAACAGATCCTCTCCTTAGACGTCGATCCTCACGAGACTGTTGAGAATGTGAAAGCTCTGCTGGAAGTGGAGACGCGGGTGCTGCTCCAGCAGCAGCAGCTGCTATATAATGGGAGGGAGATGAGGAATTCTGAGAAGCTGAGTGCTCTTGGTGTTAGAGATGAAGACTTGATAATGATGGTGTCCAATGCCGCCTCGAGTGCACCGTCCAATGCCTTGAGCTTCAATCCAGATGGGTCTGCGGTAAACCCAGGAGCTTTCCAGCAGCACATTAGGAATGATTCTAATTTGACAGCACAGTTGTTTCAGAGTGACCCAGACTTGGCACAAGTTATTCTTGGAAATGATCTCAATAGACTGCAGGAACTTTTGCGAGAGCGTCATCGCCAAAAATCTGAACTAAAACGGAAACAAGATGAGGAGCTTGCTCTTCTTTATGCAGATCCTTTTGATGTTGAAGCCCAGAAGAAGATTGAAGCTGCTATTCGCCAGAAAGGAATTGATGAAAATTGGGCTGCTGCTCTGGAGCATAACCCTGAAGCTTTTGCAAGAGTGGTTATGTTGTATGTTGACATGGAAGTGAATGGTGTCCCATTGAAGGCATTTGTTGACAGTGGAGCGCAGTCAACTATTATATCAAAAAGTTGTGCCGAGCGTTGTGGATTGTTGAGGCTTTTAGATCAACGTTATAAGGGCATTGCTCATGGAGTTGGTCAATCAGAGATATTGGGTCGAATACATGTAGCTCCAATCAAGATTGGAAGTATATTTTACCCTTGTTCATTCATGGTTCTCGATGCTCCGAATATGGAGTTTCTCTTTGGGTTGGATATGCTCCGTAAGCATCAGTGCATTATAGATTTGAACGAAAATGTTTTGAGAGTTGGCGGTGGAGAAGTTTCTGTACCATTTTTGCAAGAAAAAGACATCCCATCTCGTTTTCTCGATGAAGAAAGGTTCTCCAAGGAAGCATCCAGCTCAGGAGCTCCTGTTACAGCGGGAACAGCAGATACGAGCAAAACTGTACCATCAGGAGGGCAATCTTCGGCGAGTGCTCGTTCAAACCAGACTCAGGGATCTGAATTTGAAGCTAAGGTTGCAAAGCTTGTCGAGCTAGGGTTCGGGAGAGATGCGGTAATACAAGCTCTTAAATTTTTCGATGGTAACGAAGAACAAGCAGCCGGGTATCTTTTCGGTGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asp_protease UBA ubiquitin

Protein Analysis

410

Amino Acids

44.92

Weight (kDa)

5.01

Isoelectric Point (pI)

39.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 1 - 69 5.4e-07 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 3 - 70 1.7e-13 Ubiquitin family
Ddi2_HDD PF24669 92 - 159 9e-06 Ddi2 HDD domain
Asp_protease PF09668 188 - 296 4.4e-49 Aspartyl protease
gag-asp_proteas PF13975 199 - 290 8.4e-19 gag-polyprotein putative aspartyl protease
Asp_protease_2 PF13650 199 - 286 1.2e-10 Aspartyl protease
UBA PF00627 372 - 406 1.1e-08 UBA/TS-N domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 717
AatII GACGTC 1 cut(s) 51
AccII CGCG 2 cut(s) 23, 103
AciI CCGC 8 cut(s) 21, 23, 103, 219, 269, 915, 1025, 1160
AclI AACGTT 1 cut(s) 712
AclWI GGATC 4 cut(s) 28, 47, 464, 1111
AcsI RAATTY 3 cut(s) 154, 1109, 1178
AcuI CTGAAG 1 cut(s) 585
AcyI GRCGYC 1 cut(s) 48
AfaI GTAC 2 cut(s) 933, 1053
AfiI CCNNNNNNNGG 4 cut(s) 349, 625, 1061, 1099
AflIII ACRYGT 1 cut(s) 766
AgsI TTSAA 7 cut(s) 253, 485, 503, 625, 892, 1089, 1115
AjnI CCWGG 1 cut(s) 277
Alw21I GWGCWC 4 cut(s) 175, 232, 1015, 1084
Alw26I GTCTC 2 cut(s) 56, 92
Alw44I GTGCAC 1 cut(s) 228
AlwI GGATC 4 cut(s) 28, 47, 464, 1111
AlwNI CAGNNNCTG 1 cut(s) 127
Ama87I CYCGRG 1 cut(s) 223
ApaLI GTGCAC 1 cut(s) 228
ApoI RAATTY 3 cut(s) 154, 1109, 1178
AspLEI GCGC 1 cut(s) 649
AsuC2I CCSGG 1 cut(s) 1210
AvaI CYCGRG 1 cut(s) 223
BaeGI GKGCMC 1 cut(s) 232
BanII GRGCYC 1 cut(s) 1015
BarI GAAGNNNNNNTAC 2 cut(s) 915, 947
BauI CACGAG 1 cut(s) 59
BbsI GAAGAC 1 cut(s) 198
Bbv12I GWGCWC 4 cut(s) 175, 232, 1015, 1084
BccI CCATC 5 cut(s) 199, 254, 967, 1063, 1181
BciT130I CCWGG 1 cut(s) 279
BcnI CCSGG 1 cut(s) 1210
BcoDI GTCTC 2 cut(s) 56, 92
BfaI CTAG 1 cut(s) 1142
BfmI CTRYAG 1 cut(s) 386
Bme1390I CCNGG 2 cut(s) 279, 1210
BmeT110I CYCGRG 1 cut(s) 223
BmrFI CCNGG 2 cut(s) 279, 1210
BmsI GCATC 4 cut(s) 814, 880, 1007, 1147
BpiI GAAGAC 1 cut(s) 198
BpmI CTGGAG 2 cut(s) 98, 572
Bpu10I CCTNAGC 2 cut(s) 1006, 1119
BpuEI CTTGAG 1 cut(s) 265
BpuMI CCSGG 1 cut(s) 1210
BsaHI GRCGYC 1 cut(s) 48
BsaJI CCNNGG 3 cut(s) 21, 277, 990
BsaXI ACNNNNNCTCC 2 cut(s) 1002, 1032
Bsc4I CCNNNNNNNGG 4 cut(s) 349, 625, 1061, 1099
Bse3DI GCAATG 1 cut(s) 723
BseBI CCWGG 1 cut(s) 279
BseDI CCNNGG 3 cut(s) 21, 277, 990
BseGI GGATG 2 cut(s) 954, 998
BseLI CCNNNNNNNGG 4 cut(s) 349, 625, 1061, 1099
BseMI GCAATG 1 cut(s) 723
BseMII CTCAG 4 cut(s) 150, 158, 1020, 1112
BseRI GAGGAG 1 cut(s) 461
BseSI GKGCMC 1 cut(s) 232
Bsh1236I CGCG 2 cut(s) 23, 103
BsiHKAI GWGCWC 4 cut(s) 175, 232, 1015, 1084
BsiHKCI CYCGRG 1 cut(s) 223
BsiSI CCGG 1 cut(s) 1209
BslFI GGGAC 1 cut(s) 602
BslI CCNNNNNNNGG 4 cut(s) 349, 625, 1061, 1099
BsmAI GTCTC 2 cut(s) 56, 92
BsmBI CGTCTC 1 cut(s) 92
BsmFI GGGAC 1 cut(s) 602
BsoBI CYCGRG 1 cut(s) 223
Bsp1286I GDGCHC 4 cut(s) 175, 232, 1015, 1084
Bsp143I GATC 7 cut(s) 6, 33, 52, 373, 469, 706, 1103
BspACI CCGC 8 cut(s) 21, 23, 103, 219, 269, 915, 1025, 1160
BspCNI CTCAG 4 cut(s) 151, 159, 1019, 1111
BspFNI CGCG 2 cut(s) 23, 103
BspMAI CTGCAG 1 cut(s) 390
BspPI GGATC 4 cut(s) 28, 47, 464, 1111
BspQI GCTCTTC 1 cut(s) 462
BsrDI GCAATG 1 cut(s) 723
BssECI CCNNGG 3 cut(s) 21, 277, 990
BssMI GATC 7 cut(s) 6, 33, 52, 373, 469, 706, 1103
BssNI GRCGYC 1 cut(s) 48
BssSI CACGAG 1 cut(s) 59
BssT1I CCWWGG 1 cut(s) 990
Bst2BI CACGAG 1 cut(s) 59
Bst2UI CCWGG 1 cut(s) 279
Bst4CI ACNGT 6 cut(s) 13, 67, 234, 327, 641, 1051
Bst6I CTCTTC 1 cut(s) 462
BstACI GRCGYC 1 cut(s) 48
BstAPI GCANNNNNTGC 1 cut(s) 127
BstC8I GCNNGC 1 cut(s) 453
BstDEI CTNAG 6 cut(s) 43, 159, 167, 1006, 1098, 1119
BstDSI CCRYGG 1 cut(s) 21
BstF5I GGATG 2 cut(s) 954, 998
BstFNI CGCG 2 cut(s) 23, 103
BstHHI GCGC 1 cut(s) 649
BstKTI GATC 7 cut(s) 9, 36, 55, 376, 472, 709, 1106
BstMAI GTCTC 2 cut(s) 56, 92
BstMBI GATC 7 cut(s) 6, 33, 52, 373, 469, 706, 1103
BstNI CCWGG 1 cut(s) 279
BstNSI RCATGY 1 cut(s) 770
BstSCI CCNGG 2 cut(s) 277, 1208
BstSFI CTRYAG 1 cut(s) 386
BstSLI GKGCMC 1 cut(s) 232
BstUI CGCG 2 cut(s) 23, 103
BstV2I GAAGAC 1 cut(s) 198
BstX2I RGATCY 3 cut(s) 33, 469, 1103
BstYI RGATCY 3 cut(s) 33, 469, 1103
BtgI CCRYGG 1 cut(s) 21
BtgZI GCGATG 1 cut(s) 395
BtsCI GGATG 2 cut(s) 954, 998
BtsIMutI CAGTG 3 cut(s) 9, 646, 881
Cac8I GCNNGC 1 cut(s) 453
CaiI CAGNNNCTG 1 cut(s) 127
CfoI GCGC 1 cut(s) 649
Cfr42I CCGCGG 1 cut(s) 24
CseI GACGC 2 cut(s) 109, 395
Csp6I GTAC 2 cut(s) 932, 1052
CviAII CATG 4 cut(s) 601, 731, 767, 814
CviQI GTAC 2 cut(s) 932, 1052
DdeI CTNAG 6 cut(s) 43, 159, 167, 1006, 1098, 1119
DpnI GATC 7 cut(s) 8, 35, 54, 375, 471, 708, 1105
DpnII GATC 7 cut(s) 6, 33, 52, 373, 469, 706, 1103
Eam1104I CTCTTC 1 cut(s) 462
EarI CTCTTC 1 cut(s) 462
Ecl136II GAGCTC 1 cut(s) 1013
Eco130I CCWWGG 1 cut(s) 990
Eco24I GRGCYC 1 cut(s) 1015
Eco53kI GAGCTC 1 cut(s) 1013
Eco57I CTGAAG 1 cut(s) 585
Eco88I CYCGRG 1 cut(s) 223
EcoICRI GAGCTC 1 cut(s) 1013
EcoRI GAATTC 1 cut(s) 154
EcoRII CCWGG 1 cut(s) 277
EcoT14I CCWWGG 1 cut(s) 990
EcoT38I GRGCYC 1 cut(s) 1015
ErhI CCWWGG 1 cut(s) 990
Esp3I CGTCTC 1 cut(s) 92
FaeI CATG 4 cut(s) 604, 734, 770, 817
FalI AAGNNNNNCTT 2 cut(s) 348, 380
FaqI GGGAC 1 cut(s) 602
FatI CATG 4 cut(s) 600, 730, 766, 813
FauI CCCGC 2 cut(s) 96, 1018
FokI GGATG 2 cut(s) 941, 985
FriOI GRGCYC 1 cut(s) 1015
FspBI CTAG 1 cut(s) 1142
GlaI GCGC 1 cut(s) 648
GsuI CTGGAG 2 cut(s) 98, 572
HapII CCGG 1 cut(s) 1209
HgaI GACGC 2 cut(s) 109, 395
HhaI GCGC 1 cut(s) 649
Hin1I GRCGYC 1 cut(s) 48
Hin1II CATG 4 cut(s) 604, 734, 770, 817
Hin6I GCGC 1 cut(s) 647
HinP1I GCGC 1 cut(s) 647
HincII GTYRAC 3 cut(s) 598, 637, 654
HindII GTYRAC 3 cut(s) 598, 637, 654
HindIII AAGCTT 2 cut(s) 567, 1130
HinfI GANTC 2 cut(s) 308, 1096
HpaII CCGG 1 cut(s) 1209
Hpy166II GTNNAC 5 cut(s) 230, 274, 598, 637, 654
Hpy188I TCNGA 6 cut(s) 160, 336, 424, 748, 831, 1108
Hpy188III TCNNGA 9 cut(s) 59, 257, 551, 781, 821, 971, 1008, 1059, 1150
Hpy8I GTNNAC 5 cut(s) 230, 274, 598, 637, 654
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 1 cut(s) 619
HpyCH4III ACNGT 6 cut(s) 13, 67, 234, 327, 641, 1051
HpyCH4IV ACGT 2 cut(s) 48, 712
HpyCH4V TGCA 7 cut(s) 230, 388, 467, 575, 879, 943, 1127
HpyF3I CTNAG 6 cut(s) 43, 159, 167, 1006, 1098, 1119
HpySE526I ACGT 2 cut(s) 48, 712
Hsp92I GRCGYC 1 cut(s) 48
Hsp92II CATG 4 cut(s) 604, 734, 770, 817
HspAI GCGC 1 cut(s) 647
KspI CCGCGG 1 cut(s) 24
Kzo9I GATC 7 cut(s) 6, 33, 52, 373, 469, 706, 1103
LguI GCTCTTC 1 cut(s) 462
LweI GCATC 4 cut(s) 814, 880, 1007, 1147
MaeI CTAG 1 cut(s) 1142
MaeII ACGT 2 cut(s) 48, 712
MaeIII GTNAC 3 cut(s) 338, 1018, 1190
MalI GATC 7 cut(s) 8, 35, 54, 375, 471, 708, 1105
MboI GATC 7 cut(s) 6, 33, 52, 373, 469, 706, 1103
MboII GAAGA 8 cut(s) 16, 203, 449, 505, 508, 989, 1062, 1208
MflI RGATCY 3 cut(s) 33, 469, 1103
MhlI GDGCHC 4 cut(s) 175, 232, 1015, 1084
MluCI AATT 6 cut(s) 154, 313, 525, 535, 1109, 1178
MlyI GAGTC 1 cut(s) 1090
MmeI TCCRAC 1 cut(s) 834
MnlI CCTC 8 cut(s) 47, 66, 135, 144, 232, 439, 690, 1055
MseI TTAA 1 cut(s) 1176
MslI CAYNNNNRTG 2 cut(s) 14, 605
MspA1I CMGCKG 3 cut(s) 23, 127, 1025
MspI CCGG 1 cut(s) 1209
MspR9I CCNGG 2 cut(s) 279, 1210
MvaI CCWGG 1 cut(s) 279
MvnI CGCG 2 cut(s) 23, 103
NciI CCSGG 1 cut(s) 1210
NdeII GATC 7 cut(s) 6, 33, 52, 373, 469, 706, 1103
NlaIII CATG 4 cut(s) 604, 734, 770, 817
NmeAIII GCCGAG 1 cut(s) 703
NmuCI GTSAC 1 cut(s) 338
NspI RCATGY 1 cut(s) 770
PaeR7I CTCGAG 1 cut(s) 223
PciI ACATGT 1 cut(s) 766
PciSI GCTCTTC 1 cut(s) 462
PcsI WCGNNNNNNNCGW 3 cut(s) 57, 230, 1191
PfeI GAWTC 1 cut(s) 308
PleI GAGTC 1 cut(s) 1090
PpsI GAGTC 1 cut(s) 1090
PscI ACATGT 1 cut(s) 766
PsiI TTATAA 1 cut(s) 717
Psp124BI GAGCTC 1 cut(s) 1015
Psp1406I AACGTT 1 cut(s) 712
Psp6I CCWGG 1 cut(s) 277
PspGI CCWGG 1 cut(s) 277
PspXI VCTCGAGB 1 cut(s) 223
PsrI GAACNNNNNNTAC 2 cut(s) 1021, 1053
PstI CTGCAG 1 cut(s) 390
PstNI CAGNNNCTG 1 cut(s) 127
PsuI RGATCY 3 cut(s) 33, 469, 1103
PvuII CAGCTG 1 cut(s) 127
RsaI GTAC 2 cut(s) 933, 1053
RsaNI GTAC 2 cut(s) 932, 1052
RseI CAYNNNNRTG 2 cut(s) 14, 605
SacI GAGCTC 1 cut(s) 1015
SacII CCGCGG 1 cut(s) 24
SapI GCTCTTC 1 cut(s) 462
SaqAI TTAA 1 cut(s) 1176
Sau3AI GATC 7 cut(s) 6, 33, 52, 373, 469, 706, 1103
SchI GAGTC 1 cut(s) 1090
ScrFI CCNGG 2 cut(s) 279, 1210
SduI GDGCHC 4 cut(s) 175, 232, 1015, 1084
SfaNI GCATC 4 cut(s) 814, 880, 1007, 1147
SfcI CTRYAG 1 cut(s) 386
Sfr274I CTCGAG 1 cut(s) 223
Sfr303I CCGCGG 1 cut(s) 24
SgrBI CCGCGG 1 cut(s) 24
SlaI CTCGAG 1 cut(s) 223
SmiMI CAYNNNNRTG 2 cut(s) 14, 605
SmlI CTYRAG 2 cut(s) 223, 244
SmoI CTYRAG 2 cut(s) 223, 244
Sse9I AATT 6 cut(s) 154, 313, 525, 535, 1109, 1178
SsiI CCGC 8 cut(s) 21, 23, 103, 219, 269, 915, 1025, 1160
SspMI CTAG 1 cut(s) 1142
SstI GAGCTC 1 cut(s) 1015
StyD4I CCNGG 2 cut(s) 277, 1208
StyI CCWWGG 1 cut(s) 990
TaaI ACNGT 6 cut(s) 13, 67, 234, 327, 641, 1051
TaiI ACGT 2 cut(s) 51, 715
TaqI TCGA 7 cut(s) 51, 224, 760, 822, 972, 1137, 1185
TasI AATT 6 cut(s) 154, 313, 525, 535, 1109, 1178
TauI GCSGC 1 cut(s) 221
TfiI GAWTC 1 cut(s) 308
Tru1I TTAA 1 cut(s) 1176
Tru9I TTAA 1 cut(s) 1176
TscAI CASTG 3 cut(s) 16, 646, 881
TseFI GTSAC 1 cut(s) 338
Tsp45I GTSAC 1 cut(s) 338
TspDTI ATGAA 6 cut(s) 17, 204, 546, 798, 802, 990
TspGWI ACGGA 2 cut(s) 448, 854
TspRI CASTG 3 cut(s) 16, 646, 881
VneI GTGCAC 1 cut(s) 228
XapI RAATTY 3 cut(s) 154, 1109, 1178
XceI RCATGY 1 cut(s) 770
XhoI CTCGAG 1 cut(s) 223
XspI CTAG 1 cut(s) 1142
ZraI GACGTC 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.