Prupe.6G031700_v2.0.a1

DNA damage-inducible protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0007459
Physical Location & Seq
Reverse (-)
2452428 .. 2457288
4861 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G031700.1

Sequence Viewer

Length: 1230 bp
ATGAAGATCACTGTAATGACCGCGGACGAACAGATTCTCAACTTAGACGTGGATCCTCACGAAACTGTTGAAAATGTGAAAGCTCTGCTGGAGGTGGAGACGCGGGTGCTGCTTCAGCAACAGCAGCTGCTATATAATGGGAGGGAGATGAGGAATTCTGAGAAGCTGAGTGCCCTTGGCGTTAAAGATGAAGACTTGATAATGATGGTATCCAATGCTGCACCGAGTGCTTCAGCCAATGCTTTGAGCTTCAATCCAGATGGGTCTGCGGTAAACCCAGGAGCTTTTCAGCAGCACATTAAGAATGATTCTAATTTGATGGCACAGTTGTTTCAGAGTGACCCAGACTTAGCACAAGTTATTCTTGGAAATGATCTCAATAGACTGCAGGAACTTTTACGACAGCGTCATCTCCAAAAGTCTGAACTTCGACGGCAACAAGATGAGGAGCTTGCTCTTCTTTATGCAGATCCTTTTGATGTTGAAGCACAGAAGAAGATTGAAGCTGCTATTCGCCAGAAAGGAATTGATGAAAACTGGGCCGCGGCTCTGGAACATAATCCTGAAGCTTTTGCAAGGGTGGTTATGTTGTATGTTGACATGGAAGTCAATGGTGTCCCGTTAAAGGCATTTGTTGACAGTGGAGCGCAGTCAACAATTATTTCAAAAAGCTGCGCTGAGCGTTGCGGATTGTTGAGGCTTTTAGATCAACGTTACAAGGGTATTGCTCATGGAGTTGGTCAATCAGAGATATTGGGTCGGATACATGTAGCTCCAATCAAGATTGGGAATATATTTTATCCTTGCTCATTCTTGGTTTTGGATGCTCCCAATATGGAGTTCCTCTTTGGGTTGGATATGCTCCGTAAGCATCAGTGCATTATAGATCTGAAGGATAGTGTTTTGAGAGTTGGTGGTGGAGAAGTTTCCGTACCATTCTTGCAAGAAAAGGACATCCCATCTCGTTTTCTGGATGAAGAAAGGTTTGCCAAGGAAACATCCAGCTCAGGAGCTCCTGTTACATCTGGAAAAGCAGATACAAGCAAAAATTCACCATCGGGAGGGCAATCATCTGGAAGTGCTCGTTCTAACCCGACTCAGGGACCTGATTTTGAAGCTAAAGTTGCAAAGCTTGTTGAGCTAGGGTTCGGGAGAGATGCTGTAATACAAGCTCTTAAATTTTTCGACGGTAACGAAGAACAAGCAGCAGGATATCTTTTTGGGGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asp_protease UBA ubiquitin

Protein Analysis

410

Amino Acids

44.88

Weight (kDa)

5.04

Isoelectric Point (pI)

42.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 605
AccII CGCG 3 cut(s) 23, 103, 545
AciI CCGC 7 cut(s) 21, 23, 103, 269, 543, 545, 687
AclI AACGTT 1 cut(s) 712
AclWI GGATC 3 cut(s) 47, 60, 464
AcsI RAATTY 3 cut(s) 154, 1048, 1178
AcuI CTGAAG 4 cut(s) 98, 216, 585, 911
AdeI CACNNNGTG 1 cut(s) 227
AfaI GTAC 1 cut(s) 933
AfiI CCNNNNNNNGG 4 cut(s) 625, 1061, 1099, 1100
AflIII ACRYGT 1 cut(s) 766
AgsI TTSAA 6 cut(s) 71, 253, 485, 503, 666, 1115
AjiI CACGTC 1 cut(s) 49
AjnI CCWGG 1 cut(s) 277
AjuI GAANNNNNNNTTGG 2 cut(s) 824, 856
Alw21I GWGCWC 2 cut(s) 1015, 1084
Alw26I GTCTC 1 cut(s) 92
AlwI GGATC 3 cut(s) 47, 60, 464
AlwNI CAGNNNCTG 1 cut(s) 127
AoxI GGCC 1 cut(s) 540
ApeKI GCWGC 8 cut(s) 109, 124, 127, 218, 292, 506, 672, 1205
ApoI RAATTY 3 cut(s) 154, 1048, 1178
Asp700I GAANNNNTTC 1 cut(s) 33
AspLEI GCGC 2 cut(s) 649, 677
AspS9I GGNCC 2 cut(s) 540, 1103
AsuHPI GGTGA 1 cut(s) 1044
AvaII GGWCC 1 cut(s) 1103
BaeGI GKGCMC 1 cut(s) 175
BamHI GGATCC 1 cut(s) 52
BanII GRGCYC 1 cut(s) 1015
BarI GAAGNNNNNNTAC 2 cut(s) 915, 947
BbsI GAAGAC 1 cut(s) 198
Bbv12I GWGCWC 2 cut(s) 1015, 1084
BbvI GCAGC 8 cut(s) 96, 114, 136, 205, 304, 493, 659, 1217
BccI CCATC 5 cut(s) 199, 254, 313, 967, 1063
BceAI ACGGC 1 cut(s) 449
BciT130I CCWGG 1 cut(s) 279
BciVI GTATCC 2 cut(s) 220, 756
BcoDI GTCTC 1 cut(s) 92
BfaI CTAG 1 cut(s) 1142
BfmI CTRYAG 1 cut(s) 386
BfuI GTATCC 2 cut(s) 220, 756
BglII AGATCT 1 cut(s) 886
BlpI GCTNAGC 1 cut(s) 678
Bme1390I CCNGG 1 cut(s) 279
Bme18I GGWCC 1 cut(s) 1103
BmgBI CACGTC 1 cut(s) 49
BmgT120I GGNCC 2 cut(s) 540, 1103
BmiI GGNNCC 2 cut(s) 54, 1104
BmrFI CCNGG 1 cut(s) 279
BmrI ACTGGG 1 cut(s) 547
BmsI GCATC 3 cut(s) 814, 880, 1147
BmuI ACTGGG 1 cut(s) 547
BpiI GAAGAC 1 cut(s) 198
BpmI CTGGAG 1 cut(s) 110
Bpu10I CCTNAGC 1 cut(s) 1006
Bpu1102I GCTNAGC 1 cut(s) 678
BsaJI CCNNGG 5 cut(s) 21, 175, 277, 543, 990
BsaXI ACNNNNNCTCC 2 cut(s) 1002, 1032
Bsc4I CCNNNNNNNGG 4 cut(s) 625, 1061, 1099, 1100
Bse1I ACTGG 1 cut(s) 542
BseBI CCWGG 1 cut(s) 279
BseDI CCNNGG 5 cut(s) 21, 175, 277, 543, 990
BseGI GGATG 4 cut(s) 829, 954, 979, 998
BseLI CCNNNNNNNGG 4 cut(s) 625, 1061, 1099, 1100
BseMII CTCAG 5 cut(s) 150, 158, 669, 1020, 1112
BseNI ACTGG 1 cut(s) 542
BseRI GAGGAG 1 cut(s) 461
BseSI GKGCMC 1 cut(s) 175
BseXI GCAGC 8 cut(s) 96, 114, 136, 205, 304, 493, 659, 1217
BsgI GTGCAG 1 cut(s) 204
Bsh1236I CGCG 3 cut(s) 23, 103, 545
BshFI GGCC 1 cut(s) 542
BsiHKAI GWGCWC 2 cut(s) 1015, 1084
BslFI GGGAC 2 cut(s) 602, 1116
BslI CCNNNNNNNGG 4 cut(s) 625, 1061, 1099, 1100
BsmAI GTCTC 1 cut(s) 92
BsmBI CGTCTC 1 cut(s) 92
BsmFI GGGAC 2 cut(s) 602, 1116
BsnI GGCC 1 cut(s) 542
Bsp1286I GDGCHC 3 cut(s) 175, 1015, 1084
Bsp143I GATC 6 cut(s) 6, 52, 373, 469, 706, 886
Bsp1720I GCTNAGC 1 cut(s) 678
BspACI CCGC 7 cut(s) 21, 23, 103, 269, 543, 545, 687
BspANI GGCC 1 cut(s) 542
BspCNI CTCAG 5 cut(s) 151, 159, 670, 1019, 1111
BspFNI CGCG 3 cut(s) 23, 103, 545
BspLI GGNNCC 2 cut(s) 54, 1104
BspMAI CTGCAG 1 cut(s) 390
BspPI GGATC 3 cut(s) 47, 60, 464
BspQI GCTCTTC 1 cut(s) 462
BsrI ACTGG 1 cut(s) 542
BssECI CCNNGG 5 cut(s) 21, 175, 277, 543, 990
BssMI GATC 6 cut(s) 6, 52, 373, 469, 706, 886
BssT1I CCWWGG 2 cut(s) 175, 990
Bst2UI CCWGG 1 cut(s) 279
Bst4CI ACNGT 5 cut(s) 13, 67, 327, 641, 1190
Bst6I CTCTTC 1 cut(s) 462
BstAPI GCANNNNNTGC 1 cut(s) 227
BstC8I GCNNGC 1 cut(s) 453
BstDEI CTNAG 7 cut(s) 43, 159, 167, 349, 678, 1006, 1098
BstDSI CCRYGG 2 cut(s) 21, 543
BstF5I GGATG 4 cut(s) 829, 954, 979, 998
BstFNI CGCG 3 cut(s) 23, 103, 545
BstHHI GCGC 2 cut(s) 649, 677
BstKTI GATC 6 cut(s) 9, 55, 376, 472, 709, 889
BstMAI GTCTC 1 cut(s) 92
BstMBI GATC 6 cut(s) 6, 52, 373, 469, 706, 886
BstMWI GCNNNNNNNGC 7 cut(s) 109, 115, 124, 227, 868, 1124, 1138
BstNI CCWGG 1 cut(s) 279
BstNSI RCATGY 1 cut(s) 770
BstSCI CCNGG 1 cut(s) 277
BstSFI CTRYAG 1 cut(s) 386
BstSLI GKGCMC 1 cut(s) 175
BstUI CGCG 3 cut(s) 23, 103, 545
BstV1I GCAGC 8 cut(s) 96, 114, 136, 205, 304, 493, 659, 1217
BstV2I GAAGAC 1 cut(s) 198
BstX2I RGATCY 3 cut(s) 52, 469, 886
BstYI RGATCY 3 cut(s) 52, 469, 886
BsuI GTATCC 2 cut(s) 220, 756
BsuRI GGCC 1 cut(s) 542
BtgI CCRYGG 2 cut(s) 21, 543
BtrI CACGTC 1 cut(s) 49
BtsCI GGATG 4 cut(s) 829, 954, 979, 998
BtsIMutI CAGTG 3 cut(s) 9, 646, 881
Cac8I GCNNGC 1 cut(s) 453
CaiI CAGNNNCTG 1 cut(s) 127
CfoI GCGC 2 cut(s) 649, 677
Cfr13I GGNCC 2 cut(s) 540, 1103
Cfr42I CCGCGG 2 cut(s) 24, 546
CseI GACGC 2 cut(s) 109, 395
Csp6I GTAC 1 cut(s) 932
CviAII CATG 3 cut(s) 601, 731, 767
CviQI GTAC 1 cut(s) 932
DdeI CTNAG 7 cut(s) 43, 159, 167, 349, 678, 1006, 1098
DpnI GATC 6 cut(s) 8, 54, 375, 471, 708, 888
DpnII GATC 6 cut(s) 6, 52, 373, 469, 706, 886
DraIII CACNNNGTG 1 cut(s) 227
DrdI GACNNNNNNGTC 1 cut(s) 605
DseDI GACNNNNNNGTC 1 cut(s) 605
Eam1104I CTCTTC 1 cut(s) 462
EarI CTCTTC 1 cut(s) 462
Ecl136II GAGCTC 1 cut(s) 1013
Eco130I CCWWGG 2 cut(s) 175, 990
Eco24I GRGCYC 1 cut(s) 1015
Eco32I GATATC 1 cut(s) 1214
Eco47I GGWCC 1 cut(s) 1103
Eco53kI GAGCTC 1 cut(s) 1013
Eco57I CTGAAG 4 cut(s) 98, 216, 585, 911
EcoICRI GAGCTC 1 cut(s) 1013
EcoO109I RGGNCCY 1 cut(s) 1103
EcoRI GAATTC 1 cut(s) 154
EcoRII CCWGG 1 cut(s) 277
EcoRV GATATC 1 cut(s) 1214
EcoT14I CCWWGG 2 cut(s) 175, 990
EcoT38I GRGCYC 1 cut(s) 1015
ErhI CCWWGG 2 cut(s) 175, 990
Esp3I CGTCTC 1 cut(s) 92
FaeI CATG 3 cut(s) 604, 734, 770
FalI AAGNNNNNCTT 2 cut(s) 348, 380
FaqI GGGAC 2 cut(s) 602, 1116
FatI CATG 3 cut(s) 600, 730, 766
FauI CCCGC 1 cut(s) 96
FokI GGATG 4 cut(s) 836, 941, 985, 986
FriOI GRGCYC 1 cut(s) 1015
FspBI CTAG 1 cut(s) 1142
GlaI GCGC 2 cut(s) 648, 676
GsuI CTGGAG 1 cut(s) 110
HaeIII GGCC 1 cut(s) 542
HgaI GACGC 2 cut(s) 109, 395
HhaI GCGC 2 cut(s) 649, 677
Hin1II CATG 3 cut(s) 604, 734, 770
Hin6I GCGC 2 cut(s) 647, 675
HinP1I GCGC 2 cut(s) 647, 675
HincII GTYRAC 3 cut(s) 598, 637, 654
HindII GTYRAC 3 cut(s) 598, 637, 654
HindIII AAGCTT 2 cut(s) 567, 1130
HinfI GANTC 3 cut(s) 34, 308, 1096
HphI GGTGA 1 cut(s) 1044
Hpy166II GTNNAC 4 cut(s) 274, 598, 637, 654
Hpy188I TCNGA 6 cut(s) 160, 336, 424, 748, 762, 891
Hpy8I GTNNAC 4 cut(s) 274, 598, 637, 654
Hpy99I CGWCG 2 cut(s) 435, 1190
HpyAV CCTTC 1 cut(s) 886
HpyCH4III ACNGT 5 cut(s) 13, 67, 327, 641, 1190
HpyCH4IV ACGT 2 cut(s) 48, 712
HpyCH4V TGCA 7 cut(s) 221, 388, 467, 575, 879, 943, 1127
HpyF10VI GCNNNNNNNGC 7 cut(s) 109, 115, 124, 227, 868, 1124, 1138
HpyF3I CTNAG 7 cut(s) 43, 159, 167, 349, 678, 1006, 1098
HpySE526I ACGT 2 cut(s) 48, 712
Hsp92II CATG 3 cut(s) 604, 734, 770
HspAI GCGC 2 cut(s) 647, 675
KspI CCGCGG 2 cut(s) 24, 546
Kzo9I GATC 6 cut(s) 6, 52, 373, 469, 706, 886
LguI GCTCTTC 1 cut(s) 462
LmnI GCTCC 8 cut(s) 281, 448, 644, 778, 832, 867, 1010, 1018
Lsp1109I GCAGC 8 cut(s) 96, 114, 136, 205, 304, 493, 659, 1217
LweI GCATC 3 cut(s) 814, 880, 1147
MaeI CTAG 1 cut(s) 1142
MaeII ACGT 2 cut(s) 48, 712
MaeIII GTNAC 4 cut(s) 338, 713, 1018, 1190
MalI GATC 6 cut(s) 8, 54, 375, 471, 708, 888
MboI GATC 6 cut(s) 6, 52, 373, 469, 706, 886
MboII GAAGA 7 cut(s) 16, 203, 449, 505, 508, 989, 1208
MflI RGATCY 3 cut(s) 52, 469, 886
MhlI GDGCHC 3 cut(s) 175, 1015, 1084
MluCI AATT 6 cut(s) 154, 313, 525, 657, 1048, 1178
MlyI GAGTC 1 cut(s) 1090
MmeI TCCRAC 2 cut(s) 740, 834
MnlI CCTC 8 cut(s) 66, 85, 135, 144, 439, 690, 854, 1055
MroXI GAANNNNTTC 1 cut(s) 33
MseI TTAA 4 cut(s) 183, 300, 623, 1176
MslI CAYNNNNRTG 1 cut(s) 14
MspA1I CMGCKG 3 cut(s) 23, 127, 545
MspR9I CCNGG 1 cut(s) 279
MvaI CCWGG 1 cut(s) 279
MvnI CGCG 3 cut(s) 23, 103, 545
MwoI GCNNNNNNNGC 7 cut(s) 109, 115, 124, 227, 868, 1124, 1138
NdeII GATC 6 cut(s) 6, 52, 373, 469, 706, 886
NlaIII CATG 3 cut(s) 604, 734, 770
NlaIV GGNNCC 2 cut(s) 54, 1104
NmuCI GTSAC 1 cut(s) 338
NspI RCATGY 1 cut(s) 770
PciI ACATGT 1 cut(s) 766
PciSI GCTCTTC 1 cut(s) 462
PcsI WCGNNNNNNNCGW 1 cut(s) 1191
PdmI GAANNNNTTC 1 cut(s) 33
PfeI GAWTC 2 cut(s) 34, 308
PflFI GACNNNGTC 1 cut(s) 405
PleI GAGTC 1 cut(s) 1090
PpsI GAGTC 1 cut(s) 1090
PpuMI RGGWCCY 1 cut(s) 1103
PscI ACATGT 1 cut(s) 766
Psp124BI GAGCTC 1 cut(s) 1015
Psp1406I AACGTT 1 cut(s) 712
Psp5II RGGWCCY 1 cut(s) 1103
Psp6I CCWGG 1 cut(s) 277
PspGI CCWGG 1 cut(s) 277
PspN4I GGNNCC 2 cut(s) 54, 1104
PspPI GGNCC 2 cut(s) 540, 1103
PspPPI RGGWCCY 1 cut(s) 1103
PstI CTGCAG 1 cut(s) 390
PstNI CAGNNNCTG 1 cut(s) 127
PsuI RGATCY 3 cut(s) 52, 469, 886
PsyI GACNNNGTC 1 cut(s) 405
PvuII CAGCTG 1 cut(s) 127
RsaI GTAC 1 cut(s) 933
RsaNI GTAC 1 cut(s) 932
RseI CAYNNNNRTG 1 cut(s) 14
SacI GAGCTC 1 cut(s) 1015
SacII CCGCGG 2 cut(s) 24, 546
SapI GCTCTTC 1 cut(s) 462
SaqAI TTAA 4 cut(s) 183, 300, 623, 1176
Sau3AI GATC 6 cut(s) 6, 52, 373, 469, 706, 886
Sau96I GGNCC 2 cut(s) 540, 1103
SchI GAGTC 1 cut(s) 1090
ScrFI CCNGG 1 cut(s) 279
SduI GDGCHC 3 cut(s) 175, 1015, 1084
SfaNI GCATC 3 cut(s) 814, 880, 1147
SfcI CTRYAG 1 cut(s) 386
Sfr303I CCGCGG 2 cut(s) 24, 546
SgrBI CCGCGG 2 cut(s) 24, 546
SinI GGWCC 1 cut(s) 1103
SmiMI CAYNNNNRTG 1 cut(s) 14
Sse9I AATT 6 cut(s) 154, 313, 525, 657, 1048, 1178
SsiI CCGC 7 cut(s) 21, 23, 103, 269, 543, 545, 687
SspMI CTAG 1 cut(s) 1142
SstI GAGCTC 1 cut(s) 1015
StyD4I CCNGG 1 cut(s) 277
StyI CCWWGG 2 cut(s) 175, 990
TaaI ACNGT 5 cut(s) 13, 67, 327, 641, 1190
TaiI ACGT 2 cut(s) 51, 715
TaqI TCGA 2 cut(s) 430, 1185
TasI AATT 6 cut(s) 154, 313, 525, 657, 1048, 1178
TauI GCSGC 2 cut(s) 545, 548
TfiI GAWTC 2 cut(s) 34, 308
Tru1I TTAA 4 cut(s) 183, 300, 623, 1176
Tru9I TTAA 4 cut(s) 183, 300, 623, 1176
TscAI CASTG 3 cut(s) 16, 646, 881
TseFI GTSAC 1 cut(s) 338
TseI GCWGC 8 cut(s) 109, 124, 127, 218, 292, 506, 672, 1205
Tsp45I GTSAC 1 cut(s) 338
TspDTI ATGAA 4 cut(s) 17, 204, 546, 990
TspGWI ACGGA 2 cut(s) 854, 919
TspRI CASTG 3 cut(s) 16, 646, 881
Tth111I GACNNNGTC 1 cut(s) 405
VpaK11BI GGWCC 1 cut(s) 1103
XapI RAATTY 3 cut(s) 154, 1048, 1178
XceI RCATGY 1 cut(s) 770
XmnI GAANNNNTTC 1 cut(s) 33
XspI CTAG 1 cut(s) 1142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.