pycom11g03480

DNA damage-inducible protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0007459
Physical Location & Seq
Reverse (-)
2675902 .. 2677430
1529 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g03480.2

Sequence Viewer

Length: 648 bp
ATGCCCAGGAGAAGACATAAATTGCAACTAATTCGTATCACAGAGGATCCTTCTCTCCGTCTTCTCCAAGTGGGACTGGAAGTCCAAATTAATCAAACAAATCCTCCATTATATCCAAAAACCCGAAGCTCCGATTCGTTACAATCCACAATACAATCTCACAAAATCCTCACCAATCGACTGCGGAGATCCAATCGCAGCGACGGATTGAAGAAGATGAAGATCACAGTGATGACTGCAGACGAACAGATCCTCTCATTAGACGTCGATCCTCACGAGACTGTTGAGAATGTGAAAGCTCTGCTGGAGGTGGAGACGCGGGTGCTGCTTCATCAGCAGCAGCTGCTATATAATGGGAGGGAGATGAGGAATTCTGAGAAGCTGAGTACTCTTGGTGTCAGAGATGAAGACTTGATAATGATGGTATCCACTGCCGCTTCGAGTGCACCCACCAATGCTTTGAGCTTCAATCCGGATGGGTCTGCGGTAAATCCAGGAGCTTTCCAGCAGCACATTAAGAATGATTCTAATTTGATAGCACAGTTGTTTCAGAGTGACCCAGACTTGGCACAAGTTATTCTTGGAAATGATCGATCAATAGACTGCAGGAACTTTTACGAGAGCGTCACCGCCAAAAATCTGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asp_protease UBA ubiquitin

Protein Analysis

216

Amino Acids

24.26

Weight (kDa)

7.9

Isoelectric Point (pI)

55.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 267
AccII CGCG 1 cut(s) 319
AccIII TCCGGA 1 cut(s) 472
AciI CCGC 5 cut(s) 184, 319, 435, 485, 630
AclWI GGATC 5 cut(s) 41, 54, 183, 244, 263
AcsI RAATTY 1 cut(s) 370
AcyI GRCGYC 1 cut(s) 264
AfaI GTAC 1 cut(s) 388
AfiI CCNNNNNNNGG 1 cut(s) 565
AgsI TTSAA 2 cut(s) 211, 469
AhdI GACNNNNNGTC 1 cut(s) 80
AjnI CCWGG 2 cut(s) 5, 493
AluBI AGCT 6 cut(s) 129, 299, 343, 382, 465, 500
AluI AGCT 6 cut(s) 129, 299, 343, 382, 465, 500
Alw21I GWGCWC 1 cut(s) 448
Alw26I GTCTC 2 cut(s) 272, 308
Alw44I GTGCAC 1 cut(s) 444
AlwI GGATC 5 cut(s) 41, 54, 183, 244, 263
AlwNI CAGNNNCTG 1 cut(s) 343
Aor13HI TCCGGA 1 cut(s) 472
ApaLI GTGCAC 1 cut(s) 444
ApeKI GCWGC 6 cut(s) 198, 325, 337, 340, 343, 508
ApoI RAATTY 1 cut(s) 370
AseI ATTAAT 1 cut(s) 90
AsuHPI GGTGA 2 cut(s) 163, 619
BaeGI GKGCMC 1 cut(s) 448
BamHI GGATCC 1 cut(s) 46
BauI CACGAG 1 cut(s) 275
BbsI GAAGAC 3 cut(s) 19, 53, 414
Bbv12I GWGCWC 1 cut(s) 448
BbvI GCAGC 6 cut(s) 210, 312, 330, 349, 352, 520
BccI CCATC 2 cut(s) 415, 470
BcgI CGANNNNNNTGC 2 cut(s) 14, 48
BciT130I CCWGG 2 cut(s) 7, 495
BciVI GTATCC 1 cut(s) 436
BcoDI GTCTC 2 cut(s) 272, 308
BfmI CTRYAG 2 cut(s) 237, 604
BfuI GTATCC 1 cut(s) 436
BisI GCNGC 7 cut(s) 199, 326, 338, 341, 344, 435, 509
BlsI GCNGC 7 cut(s) 200, 327, 339, 342, 345, 436, 510
BmcAI AGTACT 1 cut(s) 388
Bme1390I CCNGG 2 cut(s) 7, 495
BmeRI GACNNNNNGTC 1 cut(s) 80
BmiI GGNNCC 1 cut(s) 48
BmrFI CCNGG 2 cut(s) 7, 495
BpiI GAAGAC 3 cut(s) 19, 53, 414
BpmI CTGGAG 1 cut(s) 326
Bsa29I ATCGAT 1 cut(s) 592
BsaBI GATNNNNATC 1 cut(s) 221
BsaHI GRCGYC 1 cut(s) 264
BsaJI CCNNGG 1 cut(s) 5
BsaWI WCCGGW 1 cut(s) 472
BsaXI ACNNNNNCTCC 2 cut(s) 88, 118
Bsc4I CCNNNNNNNGG 1 cut(s) 565
Bse1I ACTGG 1 cut(s) 81
Bse8I GATNNNNATC 1 cut(s) 221
BseAI TCCGGA 1 cut(s) 472
BseBI CCWGG 2 cut(s) 7, 495
BseCI ATCGAT 1 cut(s) 592
BseDI CCNNGG 1 cut(s) 5
BseGI GGATG 1 cut(s) 481
BseJI GATNNNNATC 1 cut(s) 221
BseLI CCNNNNNNNGG 1 cut(s) 565
BseMII CTCAG 2 cut(s) 366, 374
BseNI ACTGG 1 cut(s) 81
BseSI GKGCMC 1 cut(s) 448
BseXI GCAGC 6 cut(s) 210, 312, 330, 349, 352, 520
Bsh1236I CGCG 1 cut(s) 319
BshVI ATCGAT 1 cut(s) 592
BsiHKAI GWGCWC 1 cut(s) 448
BsiSI CCGG 1 cut(s) 473
BslFI GGGAC 1 cut(s) 87
BslI CCNNNNNNNGG 1 cut(s) 565
BsmAI GTCTC 2 cut(s) 272, 308
BsmBI CGTCTC 1 cut(s) 308
BsmFI GGGAC 1 cut(s) 87
Bsp1286I GDGCHC 1 cut(s) 448
Bsp13I TCCGGA 1 cut(s) 472
Bsp143I GATC 7 cut(s) 46, 188, 222, 249, 268, 589, 593
BspACI CCGC 5 cut(s) 184, 319, 435, 485, 630
BspCNI CTCAG 2 cut(s) 367, 375
BspDI ATCGAT 1 cut(s) 592
BspEI TCCGGA 1 cut(s) 472
BspFNI CGCG 1 cut(s) 319
BspLI GGNNCC 1 cut(s) 48
BspMAI CTGCAG 2 cut(s) 241, 608
BspPI GGATC 5 cut(s) 41, 54, 183, 244, 263
BsrI ACTGG 1 cut(s) 81
BssECI CCNNGG 1 cut(s) 5
BssMI GATC 7 cut(s) 46, 188, 222, 249, 268, 589, 593
BssNI GRCGYC 1 cut(s) 264
BssSI CACGAG 1 cut(s) 275
Bst2BI CACGAG 1 cut(s) 275
Bst2UI CCWGG 2 cut(s) 7, 495
Bst4CI ACNGT 3 cut(s) 229, 283, 543
BstACI GRCGYC 1 cut(s) 264
BstAPI GCANNNNNTGC 1 cut(s) 343
BstDEI CTNAG 2 cut(s) 375, 383
BstF5I GGATG 1 cut(s) 481
BstFNI CGCG 1 cut(s) 319
BstKTI GATC 7 cut(s) 49, 191, 225, 252, 271, 592, 596
BstMAI GTCTC 2 cut(s) 272, 308
BstMBI GATC 7 cut(s) 46, 188, 222, 249, 268, 589, 593
BstMWI GCNNNNNNNGC 4 cut(s) 325, 334, 343, 443
BstNI CCWGG 2 cut(s) 7, 495
BstSCI CCNGG 2 cut(s) 5, 493
BstSFI CTRYAG 2 cut(s) 237, 604
BstSLI GKGCMC 1 cut(s) 448
BstUI CGCG 1 cut(s) 319
BstV1I GCAGC 6 cut(s) 210, 312, 330, 349, 352, 520
BstV2I GAAGAC 3 cut(s) 19, 53, 414
BstX2I RGATCY 3 cut(s) 46, 188, 249
BstYI RGATCY 3 cut(s) 46, 188, 249
Bsu15I ATCGAT 1 cut(s) 592
BsuI GTATCC 1 cut(s) 436
BsuTUI ATCGAT 1 cut(s) 592
BtsCI GGATG 1 cut(s) 481
BtsI GCAGTG 1 cut(s) 429
BtsIMutI CAGTG 2 cut(s) 234, 429
CaiI CAGNNNCTG 1 cut(s) 343
ClaI ATCGAT 1 cut(s) 592
CseI GACGC 2 cut(s) 325, 613
Csp6I GTAC 1 cut(s) 387
CviJI RGCY 6 cut(s) 129, 299, 343, 382, 465, 500
CviKI_1 RGCY 6 cut(s) 129, 299, 343, 382, 465, 500
CviQI GTAC 1 cut(s) 387
DdeI CTNAG 2 cut(s) 375, 383
DpnI GATC 7 cut(s) 48, 190, 224, 251, 270, 591, 595
DpnII GATC 7 cut(s) 46, 188, 222, 249, 268, 589, 593
DriI GACNNNNNGTC 1 cut(s) 80
Eam1105I GACNNNNNGTC 1 cut(s) 80
EcoRI GAATTC 1 cut(s) 370
EcoRII CCWGG 2 cut(s) 5, 493
Esp3I CGTCTC 1 cut(s) 308
FaiI YATR 4 cut(s) 18, 112, 349, 351
FalI AAGNNNNNCTT 2 cut(s) 564, 596
FaqI GGGAC 1 cut(s) 87
FauI CCCGC 1 cut(s) 312
Fnu4HI GCNGC 7 cut(s) 199, 326, 338, 341, 344, 435, 509
FokI GGATG 1 cut(s) 488
Fsp4HI GCNGC 7 cut(s) 199, 326, 338, 341, 344, 435, 509
GluI GCNGC 7 cut(s) 199, 326, 338, 341, 344, 435, 509
GsuI CTGGAG 1 cut(s) 326
HapII CCGG 1 cut(s) 473
HgaI GACGC 2 cut(s) 325, 613
Hin1I GRCGYC 1 cut(s) 264
HinfI GANTC 2 cut(s) 134, 524
HpaII CCGG 1 cut(s) 473
HphI GGTGA 2 cut(s) 163, 619
Hpy166II GTNNAC 1 cut(s) 446
Hpy188I TCNGA 5 cut(s) 133, 376, 401, 552, 642
Hpy188III TCNNGA 2 cut(s) 275, 473
Hpy8I GTNNAC 1 cut(s) 446
Hpy99I CGWCG 2 cut(s) 206, 269
HpyAV CCTTC 1 cut(s) 60
HpyCH4III ACNGT 3 cut(s) 229, 283, 543
HpyCH4IV ACGT 1 cut(s) 264
HpyCH4V TGCA 4 cut(s) 25, 239, 446, 606
HpyF10VI GCNNNNNNNGC 4 cut(s) 325, 334, 343, 443
HpyF3I CTNAG 2 cut(s) 375, 383
HpySE526I ACGT 1 cut(s) 264
Hsp92I GRCGYC 1 cut(s) 264
Kpn2I TCCGGA 1 cut(s) 472
Kzo9I GATC 7 cut(s) 46, 188, 222, 249, 268, 589, 593
LmnI GCTCC 2 cut(s) 134, 497
LpnPI CCDG 9 cut(s) 19, 62, 290, 480, 486, 507, 518, 573, 592
Lsp1109I GCAGC 6 cut(s) 210, 312, 330, 349, 352, 520
MaeII ACGT 1 cut(s) 264
MaeIII GTNAC 3 cut(s) 138, 554, 625
MalI GATC 7 cut(s) 48, 190, 224, 251, 270, 591, 595
MboI GATC 7 cut(s) 46, 188, 222, 249, 268, 589, 593
MboII GAAGA 6 cut(s) 24, 53, 223, 226, 232, 419
MflI RGATCY 3 cut(s) 46, 188, 249
MhlI GDGCHC 1 cut(s) 448
MluCI AATT 5 cut(s) 20, 30, 87, 370, 529
MnlI CCTC 8 cut(s) 37, 114, 179, 263, 282, 301, 351, 360
MroI TCCGGA 1 cut(s) 472
MseI TTAA 2 cut(s) 90, 516
MslI CAYNNNNRTG 1 cut(s) 230
MspA1I CMGCKG 1 cut(s) 343
MspI CCGG 1 cut(s) 473
MspR9I CCNGG 2 cut(s) 7, 495
MvaI CCWGG 2 cut(s) 7, 495
MvnI CGCG 1 cut(s) 319
MwoI GCNNNNNNNGC 4 cut(s) 325, 334, 343, 443
NdeII GATC 7 cut(s) 46, 188, 222, 249, 268, 589, 593
NlaIV GGNNCC 1 cut(s) 48
NmuCI GTSAC 2 cut(s) 554, 625
PcsI WCGNNNNNNNCGW 1 cut(s) 273
PfeI GAWTC 2 cut(s) 134, 524
PfoI TCCNGGA 1 cut(s) 493
PkrI GCNGC 7 cut(s) 200, 327, 339, 342, 345, 436, 510
PshBI ATTAAT 1 cut(s) 90
Psp6I CCWGG 2 cut(s) 5, 493
PspGI CCWGG 2 cut(s) 5, 493
PspN4I GGNNCC 1 cut(s) 48
PstI CTGCAG 2 cut(s) 241, 608
PstNI CAGNNNCTG 1 cut(s) 343
PsuI RGATCY 3 cut(s) 46, 188, 249
PvuII CAGCTG 1 cut(s) 343
RsaI GTAC 1 cut(s) 388
RsaNI GTAC 1 cut(s) 387
RseI CAYNNNNRTG 1 cut(s) 230
SaqAI TTAA 2 cut(s) 90, 516
SatI GCNGC 7 cut(s) 199, 326, 338, 341, 344, 435, 509
Sau3AI GATC 7 cut(s) 46, 188, 222, 249, 268, 589, 593
ScaI AGTACT 1 cut(s) 388
ScrFI CCNGG 2 cut(s) 7, 495
SduI GDGCHC 1 cut(s) 448
SetI ASST 8 cut(s) 131, 267, 301, 312, 345, 384, 467, 502
SfcI CTRYAG 2 cut(s) 237, 604
SmiMI CAYNNNNRTG 1 cut(s) 230
Sse9I AATT 5 cut(s) 20, 30, 87, 370, 529
SsiI CCGC 5 cut(s) 184, 319, 435, 485, 630
StyD4I CCNGG 2 cut(s) 5, 493
TaaI ACNGT 3 cut(s) 229, 283, 543
TaiI ACGT 1 cut(s) 267
TaqI TCGA 4 cut(s) 178, 267, 440, 592
TasI AATT 5 cut(s) 20, 30, 87, 370, 529
TatI WGTACW 1 cut(s) 386
TauI GCSGC 1 cut(s) 437
TfiI GAWTC 2 cut(s) 134, 524
Tru1I TTAA 2 cut(s) 90, 516
Tru9I TTAA 2 cut(s) 90, 516
TscAI CASTG 2 cut(s) 234, 436
TseFI GTSAC 2 cut(s) 554, 625
TseI GCWGC 6 cut(s) 198, 325, 337, 340, 343, 508
Tsp45I GTSAC 2 cut(s) 554, 625
TspDTI ATGAA 3 cut(s) 233, 320, 420
TspGWI ACGGA 2 cut(s) 47, 219
TspRI CASTG 2 cut(s) 234, 436
VneI GTGCAC 1 cut(s) 444
VspI ATTAAT 1 cut(s) 90
XapI RAATTY 1 cut(s) 370
ZraI GACGTC 1 cut(s) 265
ZrmI AGTACT 1 cut(s) 388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.