MD11G1040100.v1.1

DNA damage-inducible protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0007459
Physical Location & Seq
Reverse (-)
3454180 .. 3459117
4938 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1040100.v1.1.491

Sequence Viewer

Length: 1230 bp
ATGAAGATCACAGTGATGACTGCGGACGAACAGATCCTCTCGTTAGACGTCGATCCTCACGAGACTGTTGAGAATGTGAAAGCTCTGCTGGAGGTGGAGACGCGGGTGCTGCTTCATCAGCAGCAGCTGCTATATAATGGGAGGGAGATGAGGAATTCTGAGAAGCTGAGTGCTCTTGGTGTCAGAGATGAAGACTTGATAATGATGGTATCCACTACCGCTTCGAGTGCACCCACCAATGCTTTGAGCTTCAATCCGGATGGGTCTGCGGTAAATCCAGAAGCTTTCCAGCAGCACATTAGGAATGATTCTAATTTGATAGCACAGTTGTTTCAGAGTGACCCAGACTTGGCACAAGTTATTCTTGGAAATGATCTCAATAGACTGCAGGAACTTTTAAGAGAGCGTCATCGCCAAAAATCTGAACTAAAACAGAAACAAGATGAGGAGCTTGCTCTTCTTTATGCAGATCCTTTTGATGTTGAAGCCCAGAAGAAGATTGAAGCTGCTATTCGCCAGAAAGGAATTGATGAAAATTGGGCTGCTGCTTTGGAGCATAACCCTGAAGCTTTCGCAAGAGTGGTTATGTTGTATGTTGACATGGAAGTGAATGGTATCCCGTTGAAGGCATTTGTTGATAGTGGAGCGCAGTCAACTATTATATCTAAAAGTTGTGCCGAGCGTTGTGGATTGTTGAGGCTTTTAGATCAACGTTATAAGGGCATTGCTCATGGAGTTGGTCAATCCGAGATATTGGGTCGAATACATGTAGCTCCTATCAAGATTGGAAGTATATTTTACCCTTGTTCATTCATGGTTCTCGATGCTCCAAATATGGAGTTTCTCTTTGGGTTGGATATGCTCCGTAAGCATCAGTGCATTATAGATTTGAATGAAAATGTTTTGAGAGTTGGCGGTGGAGAAGTTTCTGTACCATTTTTGCAAGAAAAAGACATCCCATCTCGTTTTCTCGATGAAGAAAGGTTTGCCAAGGAAGCATCCAGCTCAGGAGCTCCTGTTACACCGGGAACAGCAGATACGAGCAAAACTGTGCCATCTGGAGGGCAATCTTCTGGGAGTGCTCGTTCTAACCCGACTCAGGGATCTGATTTTGAAGCTAAAGTTGCAAAGCTTGTTGAGCTAGGGTTTGGGAGAGATGCGGTAATACAAGCTCTTAAATTTTTTGACGGTAACGAAGAACAAGCAGCCGGGTACCTTTTCGGAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asp_protease UBA ubiquitin

Protein Analysis

410

Amino Acids

44.99

Weight (kDa)

4.96

Isoelectric Point (pI)

36.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 1 - 69 2e-06 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 3 - 70 1e-13 Ubiquitin family
Ddi2_HDD PF24669 92 - 159 1.6e-06 Ddi2 HDD domain
Asp_protease PF09668 188 - 296 1.1e-48 Aspartyl protease
gag-asp_proteas PF13975 199 - 290 2.6e-18 gag-polyprotein putative aspartyl protease
Asp_protease_2 PF13650 199 - 286 3e-10 Aspartyl protease
UBA PF00627 372 - 406 1.6e-08 UBA/TS-N domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 717
AatII GACGTC 1 cut(s) 51
Acc65I GGTACC 1 cut(s) 1212
AccB1I GGYRCC 1 cut(s) 1212
AccII CGCG 1 cut(s) 103
AccIII TCCGGA 1 cut(s) 256
AciI CCGC 6 cut(s) 23, 103, 219, 269, 915, 1160
AclI AACGTT 1 cut(s) 712
AclWI GGATC 4 cut(s) 28, 47, 464, 1111
AcsI RAATTY 2 cut(s) 154, 1178
AcuI CTGAAG 1 cut(s) 585
AcyI GRCGYC 1 cut(s) 48
AfaI GTAC 2 cut(s) 933, 1214
AfiI CCNNNNNNNGG 5 cut(s) 349, 625, 1061, 1099, 1100
AflIII ACRYGT 1 cut(s) 766
AgsI TTSAA 6 cut(s) 253, 485, 503, 625, 892, 1115
AloI GAACNNNNNNTCC 2 cut(s) 1069, 1101
Alw21I GWGCWC 4 cut(s) 175, 232, 1015, 1084
Alw26I GTCTC 2 cut(s) 56, 92
Alw44I GTGCAC 1 cut(s) 228
AlwI GGATC 4 cut(s) 28, 47, 464, 1111
AlwNI CAGNNNCTG 1 cut(s) 127
Aor13HI TCCGGA 1 cut(s) 256
ApaLI GTGCAC 1 cut(s) 228
ApeKI GCWGC 9 cut(s) 109, 121, 124, 127, 292, 506, 542, 545, 1205
ApoI RAATTY 2 cut(s) 154, 1178
Asp718I GGTACC 1 cut(s) 1212
AspLEI GCGC 1 cut(s) 649
AsuC2I CCSGG 2 cut(s) 1026, 1210
BaeGI GKGCMC 1 cut(s) 232
BanI GGYRCC 1 cut(s) 1212
BanII GRGCYC 1 cut(s) 1015
BarI GAAGNNNNNNTAC 2 cut(s) 915, 947
BauI CACGAG 1 cut(s) 59
BbsI GAAGAC 1 cut(s) 198
Bbv12I GWGCWC 4 cut(s) 175, 232, 1015, 1084
BbvI GCAGC 9 cut(s) 96, 114, 133, 136, 304, 493, 529, 532, 1217
BccI CCATC 4 cut(s) 199, 254, 967, 1063
BciVI GTATCC 2 cut(s) 220, 626
BcnI CCSGG 2 cut(s) 1026, 1210
BcoDI GTCTC 2 cut(s) 56, 92
BfaI CTAG 1 cut(s) 1142
BfmI CTRYAG 1 cut(s) 386
BfuI GTATCC 2 cut(s) 220, 626
BisI GCNGC 9 cut(s) 110, 122, 125, 128, 293, 507, 543, 546, 1206
BlsI GCNGC 9 cut(s) 111, 123, 126, 129, 294, 508, 544, 547, 1207
Bme1390I CCNGG 2 cut(s) 1026, 1210
BmiI GGNNCC 1 cut(s) 1214
BmrFI CCNGG 2 cut(s) 1026, 1210
BmsI GCATC 4 cut(s) 814, 880, 1007, 1147
BpiI GAAGAC 1 cut(s) 198
BpmI CTGGAG 2 cut(s) 110, 1080
Bpu10I CCTNAGC 1 cut(s) 1006
BpuMI CCSGG 2 cut(s) 1026, 1210
BsaHI GRCGYC 1 cut(s) 48
BsaJI CCNNGG 1 cut(s) 990
BsaWI WCCGGW 1 cut(s) 256
BsaXI ACNNNNNCTCC 4 cut(s) 1002, 1032, 1069, 1099
Bsc4I CCNNNNNNNGG 5 cut(s) 349, 625, 1061, 1099, 1100
Bse3DI GCAATG 1 cut(s) 723
BseAI TCCGGA 1 cut(s) 256
BseDI CCNNGG 1 cut(s) 990
BseGI GGATG 3 cut(s) 265, 954, 998
BseLI CCNNNNNNNGG 5 cut(s) 349, 625, 1061, 1099, 1100
BseMI GCAATG 1 cut(s) 723
BseMII CTCAG 4 cut(s) 150, 158, 1020, 1112
BseRI GAGGAG 1 cut(s) 461
BseSI GKGCMC 1 cut(s) 232
BseXI GCAGC 9 cut(s) 96, 114, 133, 136, 304, 493, 529, 532, 1217
Bsh1236I CGCG 1 cut(s) 103
BshNI GGYRCC 1 cut(s) 1212
BsiHKAI GWGCWC 4 cut(s) 175, 232, 1015, 1084
BsiSI CCGG 3 cut(s) 257, 1025, 1209
BslI CCNNNNNNNGG 5 cut(s) 349, 625, 1061, 1099, 1100
BsmAI GTCTC 2 cut(s) 56, 92
BsmBI CGTCTC 1 cut(s) 92
Bsp1286I GDGCHC 4 cut(s) 175, 232, 1015, 1084
Bsp13I TCCGGA 1 cut(s) 256
Bsp143I GATC 7 cut(s) 6, 33, 52, 373, 469, 706, 1103
BspACI CCGC 6 cut(s) 23, 103, 219, 269, 915, 1160
BspCNI CTCAG 4 cut(s) 151, 159, 1019, 1111
BspEI TCCGGA 1 cut(s) 256
BspFNI CGCG 1 cut(s) 103
BspLI GGNNCC 1 cut(s) 1214
BspMAI CTGCAG 1 cut(s) 390
BspPI GGATC 4 cut(s) 28, 47, 464, 1111
BspQI GCTCTTC 1 cut(s) 462
BspT107I GGYRCC 1 cut(s) 1212
BsrDI GCAATG 1 cut(s) 723
BssECI CCNNGG 1 cut(s) 990
BssMI GATC 7 cut(s) 6, 33, 52, 373, 469, 706, 1103
BssNI GRCGYC 1 cut(s) 48
BssSI CACGAG 1 cut(s) 59
BssT1I CCWWGG 1 cut(s) 990
Bst2BI CACGAG 1 cut(s) 59
Bst4CI ACNGT 5 cut(s) 13, 67, 327, 1051, 1190
Bst6I CTCTTC 1 cut(s) 462
BstACI GRCGYC 1 cut(s) 48
BstAPI GCANNNNNTGC 1 cut(s) 127
BstC8I GCNNGC 1 cut(s) 453
BstDEI CTNAG 4 cut(s) 159, 167, 1006, 1098
BstF5I GGATG 3 cut(s) 265, 954, 998
BstFNI CGCG 1 cut(s) 103
BstHHI GCGC 1 cut(s) 649
BstKTI GATC 7 cut(s) 9, 36, 55, 376, 472, 709, 1106
BstMAI GTCTC 2 cut(s) 56, 92
BstMBI GATC 7 cut(s) 6, 33, 52, 373, 469, 706, 1103
BstMWI GCNNNNNNNGC 8 cut(s) 109, 118, 127, 227, 868, 995, 1124, 1138
BstNSI RCATGY 1 cut(s) 770
BstSCI CCNGG 2 cut(s) 1024, 1208
BstSFI CTRYAG 1 cut(s) 386
BstSLI GKGCMC 1 cut(s) 232
BstUI CGCG 1 cut(s) 103
BstV1I GCAGC 9 cut(s) 96, 114, 133, 136, 304, 493, 529, 532, 1217
BstV2I GAAGAC 1 cut(s) 198
BstX2I RGATCY 3 cut(s) 33, 469, 1103
BstYI RGATCY 3 cut(s) 33, 469, 1103
BsuI GTATCC 2 cut(s) 220, 626
BtgZI GCGATG 1 cut(s) 395
BtsCI GGATG 3 cut(s) 265, 954, 998
BtsIMutI CAGTG 2 cut(s) 18, 881
Cac8I GCNNGC 1 cut(s) 453
CaiI CAGNNNCTG 1 cut(s) 127
CfoI GCGC 1 cut(s) 649
CseI GACGC 2 cut(s) 109, 395
Csp6I GTAC 2 cut(s) 932, 1213
CviAII CATG 4 cut(s) 601, 731, 767, 814
CviQI GTAC 2 cut(s) 932, 1213
DdeI CTNAG 4 cut(s) 159, 167, 1006, 1098
DpnI GATC 7 cut(s) 8, 35, 54, 375, 471, 708, 1105
DpnII GATC 7 cut(s) 6, 33, 52, 373, 469, 706, 1103
Eam1104I CTCTTC 1 cut(s) 462
EarI CTCTTC 1 cut(s) 462
Ecl136II GAGCTC 1 cut(s) 1013
Eco130I CCWWGG 1 cut(s) 990
Eco24I GRGCYC 1 cut(s) 1015
Eco53kI GAGCTC 1 cut(s) 1013
Eco57I CTGAAG 1 cut(s) 585
EcoICRI GAGCTC 1 cut(s) 1013
EcoRI GAATTC 1 cut(s) 154
EcoT14I CCWWGG 1 cut(s) 990
EcoT38I GRGCYC 1 cut(s) 1015
ErhI CCWWGG 1 cut(s) 990
Esp3I CGTCTC 1 cut(s) 92
FaeI CATG 4 cut(s) 604, 734, 770, 817
FalI AAGNNNNNCTT 2 cut(s) 348, 380
FatI CATG 4 cut(s) 600, 730, 766, 813
FauI CCCGC 1 cut(s) 96
Fnu4HI GCNGC 9 cut(s) 110, 122, 125, 128, 293, 507, 543, 546, 1206
FokI GGATG 3 cut(s) 272, 941, 985
FriOI GRGCYC 1 cut(s) 1015
Fsp4HI GCNGC 9 cut(s) 110, 122, 125, 128, 293, 507, 543, 546, 1206
FspBI CTAG 1 cut(s) 1142
GlaI GCGC 1 cut(s) 648
GluI GCNGC 9 cut(s) 110, 122, 125, 128, 293, 507, 543, 546, 1206
GsuI CTGGAG 2 cut(s) 110, 1080
HapII CCGG 3 cut(s) 257, 1025, 1209
HgaI GACGC 2 cut(s) 109, 395
HhaI GCGC 1 cut(s) 649
Hin1I GRCGYC 1 cut(s) 48
Hin1II CATG 4 cut(s) 604, 734, 770, 817
Hin6I GCGC 1 cut(s) 647
HinP1I GCGC 1 cut(s) 647
HincII GTYRAC 2 cut(s) 598, 654
HindII GTYRAC 2 cut(s) 598, 654
HindIII AAGCTT 3 cut(s) 282, 567, 1130
HinfI GANTC 2 cut(s) 308, 1096
HpaII CCGG 3 cut(s) 257, 1025, 1209
Hpy166II GTNNAC 3 cut(s) 230, 598, 654
Hpy188I TCNGA 7 cut(s) 160, 185, 336, 424, 748, 1108, 1223
Hpy188III TCNNGA 8 cut(s) 59, 257, 278, 781, 821, 971, 1008, 1059
Hpy8I GTNNAC 3 cut(s) 230, 598, 654
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 1 cut(s) 619
HpyCH4III ACNGT 5 cut(s) 13, 67, 327, 1051, 1190
HpyCH4IV ACGT 2 cut(s) 48, 712
HpyCH4V TGCA 6 cut(s) 230, 388, 467, 879, 943, 1127
HpyF10VI GCNNNNNNNGC 8 cut(s) 109, 118, 127, 227, 868, 995, 1124, 1138
HpyF3I CTNAG 4 cut(s) 159, 167, 1006, 1098
HpySE526I ACGT 2 cut(s) 48, 712
Hsp92I GRCGYC 1 cut(s) 48
Hsp92II CATG 4 cut(s) 604, 734, 770, 817
HspAI GCGC 1 cut(s) 647
Kpn2I TCCGGA 1 cut(s) 256
KpnI GGTACC 1 cut(s) 1216
Kzo9I GATC 7 cut(s) 6, 33, 52, 373, 469, 706, 1103
LguI GCTCTTC 1 cut(s) 462
LmnI GCTCC 8 cut(s) 448, 553, 644, 778, 832, 867, 1010, 1018
Lsp1109I GCAGC 9 cut(s) 96, 114, 133, 136, 304, 493, 529, 532, 1217
LweI GCATC 4 cut(s) 814, 880, 1007, 1147
MaeI CTAG 1 cut(s) 1142
MaeII ACGT 2 cut(s) 48, 712
MaeIII GTNAC 3 cut(s) 338, 1018, 1190
MalI GATC 7 cut(s) 8, 35, 54, 375, 471, 708, 1105
MboI GATC 7 cut(s) 6, 33, 52, 373, 469, 706, 1103
MboII GAAGA 8 cut(s) 16, 203, 449, 505, 508, 989, 1062, 1208
MflI RGATCY 3 cut(s) 33, 469, 1103
MhlI GDGCHC 4 cut(s) 175, 232, 1015, 1084
MluCI AATT 5 cut(s) 154, 313, 525, 535, 1178
MlyI GAGTC 1 cut(s) 1090
MmeI TCCRAC 1 cut(s) 834
MnlI CCTC 9 cut(s) 47, 66, 85, 135, 144, 439, 690, 1055, 1217
MroI TCCGGA 1 cut(s) 256
MseI TTAA 2 cut(s) 398, 1176
MslI CAYNNNNRTG 2 cut(s) 14, 605
MspA1I CMGCKG 1 cut(s) 127
MspI CCGG 3 cut(s) 257, 1025, 1209
MspR9I CCNGG 2 cut(s) 1026, 1210
MvnI CGCG 1 cut(s) 103
MwoI GCNNNNNNNGC 8 cut(s) 109, 118, 127, 227, 868, 995, 1124, 1138
NciI CCSGG 2 cut(s) 1026, 1210
NdeII GATC 7 cut(s) 6, 33, 52, 373, 469, 706, 1103
NlaIII CATG 4 cut(s) 604, 734, 770, 817
NlaIV GGNNCC 1 cut(s) 1214
NmeAIII GCCGAG 1 cut(s) 703
NmuCI GTSAC 1 cut(s) 338
NspI RCATGY 1 cut(s) 770
PciI ACATGT 1 cut(s) 766
PciSI GCTCTTC 1 cut(s) 462
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PfeI GAWTC 1 cut(s) 308
PkrI GCNGC 9 cut(s) 111, 123, 126, 129, 294, 508, 544, 547, 1207
PleI GAGTC 1 cut(s) 1090
PpsI GAGTC 1 cut(s) 1090
PscI ACATGT 1 cut(s) 766
PsiI TTATAA 1 cut(s) 717
Psp124BI GAGCTC 1 cut(s) 1015
Psp1406I AACGTT 1 cut(s) 712
PspN4I GGNNCC 1 cut(s) 1214
PsrI GAACNNNNNNTAC 2 cut(s) 1021, 1053
PstI CTGCAG 1 cut(s) 390
PstNI CAGNNNCTG 1 cut(s) 127
PsuI RGATCY 3 cut(s) 33, 469, 1103
PvuII CAGCTG 1 cut(s) 127
RsaI GTAC 2 cut(s) 933, 1214
RsaNI GTAC 2 cut(s) 932, 1213
RseI CAYNNNNRTG 2 cut(s) 14, 605
SacI GAGCTC 1 cut(s) 1015
SapI GCTCTTC 1 cut(s) 462
SaqAI TTAA 2 cut(s) 398, 1176
SatI GCNGC 9 cut(s) 110, 122, 125, 128, 293, 507, 543, 546, 1206
Sau3AI GATC 7 cut(s) 6, 33, 52, 373, 469, 706, 1103
SchI GAGTC 1 cut(s) 1090
ScrFI CCNGG 2 cut(s) 1026, 1210
SduI GDGCHC 4 cut(s) 175, 232, 1015, 1084
SfaNI GCATC 4 cut(s) 814, 880, 1007, 1147
SfcI CTRYAG 1 cut(s) 386
SmiMI CAYNNNNRTG 2 cut(s) 14, 605
Sse9I AATT 5 cut(s) 154, 313, 525, 535, 1178
SsiI CCGC 6 cut(s) 23, 103, 219, 269, 915, 1160
SspMI CTAG 1 cut(s) 1142
SstI GAGCTC 1 cut(s) 1015
StyD4I CCNGG 2 cut(s) 1024, 1208
StyI CCWWGG 1 cut(s) 990
TaaI ACNGT 5 cut(s) 13, 67, 327, 1051, 1190
TaiI ACGT 2 cut(s) 51, 715
TaqI TCGA 5 cut(s) 51, 224, 760, 822, 972
TasI AATT 5 cut(s) 154, 313, 525, 535, 1178
TfiI GAWTC 1 cut(s) 308
Tru1I TTAA 2 cut(s) 398, 1176
Tru9I TTAA 2 cut(s) 398, 1176
TscAI CASTG 2 cut(s) 18, 881
TseFI GTSAC 1 cut(s) 338
TseI GCWGC 9 cut(s) 109, 121, 124, 127, 292, 506, 542, 545, 1205
Tsp45I GTSAC 1 cut(s) 338
TspDTI ATGAA 8 cut(s) 17, 104, 204, 546, 798, 802, 909, 990
TspGWI ACGGA 1 cut(s) 854
TspRI CASTG 2 cut(s) 18, 881
VneI GTGCAC 1 cut(s) 228
XapI RAATTY 2 cut(s) 154, 1178
XceI RCATGY 1 cut(s) 770
XspI CTAG 1 cut(s) 1142
ZraI GACGTC 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.