AT3G14870

Plant protein of unknown function (DUF641)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0004482
Physical Location & Seq
Forward (+)
5002271 .. 5005877
3607 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G14870.4

Sequence Viewer

Length: 1428 bp
ATGAATCTGCAGATGGAGTCTGTGAAACCATTGGATGTTGTTTCTTCAGGAAAAGGAAAACTCAGACGCACATTTGCTAAAGTCATCAACATGAAAAAGCTAACCGGTGTTGTTCCAGAAGGTAACAACAAGGTTGAAAGAGTGAAGAAGAGCCAAGAAAAGGTGAAGTTGGATAAAGATTTAGCAAAGAATGCTGCAAATCTGTCTGAGTCCTTTGACAAGCTGGAGGAAGAGTATGAGAAAAGACTGGCCATGGAGGCTCTTCTTGCAAAGCTGTTTGCTACGATTTCTTCGATAAAATCTGGTTATGCGCAGTTGCAGTATGCTCAGTCTCCTTATGATCCAAATGGGATTCAGAAAGCAGATAACTTGGTAGTAGCAGAGCTGAAGACATTATCAGAGCTGAAACAGAGTTTCTTGAAGAAACAGCTTGATCCTAATCCTGATAGAACTCTTGTTCTTGCGGAGATTCAAGAGCTAAGAAGCGTCTTAAAGACTTATGAGATCATGGGGAAGAAGCTGGAGTGTCAGTTAAAGCTCAAAGACTCTGAGATCATATTTCTCAAAGAAAAGTTTCAAGAATCCATGACTCAGAACAAGTTGATGGAGAAGAGATTAAACCAAAGTGGCCAACTTTGCAATCCATTGGATCATAATCTTCATCTATCGGCGGTAAGTTCGACCCATTTCGTTACTTATCTGCACCACACTGTCAAATCCATCCGAGGATTCGTCAAGTTGATGGTTGAGCAGATGAAACTCGCTGCTTGGGACATCGATATGGCTGCTGAGTTAATTCAGCCTGATGTACTCTATTACAAGCAAGACCACAAGTGTTTTGCCTTGGAGCATTATGTCTGCAAGATAATGCTTGAAGCTTTTCAACTACCTTACTTCTCAAACGAATCATCGAAGAAGACGAGCAGAGAAGACAAAGCAATGTTCTTTGAGAGATTCACAGAGCTTAGATCGATGAAACCAAGAGAGTATTTAGCATCTCGGCCTAAATCAAGATTGGCTAAGTTTTGCAGAACTAAGTATTTGCAGCTCATACATCCAAAGATGGAGCAGGCATTCTTCGGACATTTGCATCAAAGAAACCAAGTCACTGCAGGGGAGTTTCCAGAGACGAGCTTGTGCACTGCGTTTCTCGAAATGGCGAAAAGGGTTTGGCTTTTACATTGCCTTGCTTTCTCCTTCGATCCTGAAGCTTCAATCTTTCAAGTATCTAGAGGTTGCAGATTCTCCGAAGTGTACATGAAGAGCGTGTCGGAGGAAGCGTTTTTCTCACCGGAGCAAGAAGAAAGCTCGTCGGAAACTGAACCGGGGGTTGCGTTTACGGTTGTTCCTGGGTTTAGAATTGGGAAAACGACGATACAATGTGAGGTCTACCTCTCGCGTTCATGTCAACGCCGTCAGAGCAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF641

Protein Analysis

475

Amino Acids

54.74

Weight (kDa)

9.07

Isoelectric Point (pI)

50.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF641 PF04859 77 - 201 2.8e-45 Plant protein of unknown function (DUF641)
GIL1_IRKI_C PF24994 402 - 460 6e-22 GIL1/IRKI C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 312
AccI GTMKAC 1 cut(s) 1389
AccII CGCG 1 cut(s) 1399
AciI CCGC 2 cut(s) 464, 671
AclWI GGATC 4 cut(s) 335, 428, 657, 1196
AcoI YGGCCR 2 cut(s) 249, 628
AcuI CTGAAG 3 cut(s) 30, 407, 1227
AfaI GTAC 2 cut(s) 810, 1256
AfiI CCNNNNNNNGG 1 cut(s) 160
AgeI ACCGGT 1 cut(s) 104
AgsI TTSAA 8 cut(s) 137, 421, 473, 578, 875, 884, 1215, 1223
AjnI CCWGG 1 cut(s) 1348
Alw21I GWGCWC 1 cut(s) 1142
Alw26I GTCTC 2 cut(s) 336, 1121
Alw44I GTGCAC 1 cut(s) 1138
AlwI GGATC 4 cut(s) 335, 428, 657, 1196
AoxI GGCC 3 cut(s) 249, 628, 1001
ApaLI GTGCAC 1 cut(s) 1138
ApeKI GCWGC 4 cut(s) 194, 764, 785, 1045
AsiGI ACCGGT 1 cut(s) 104
Asp700I GAANNNNTTC 2 cut(s) 573, 879
AspLEI GCGC 1 cut(s) 313
AsuC2I CCSGG 1 cut(s) 1326
AsuHPI GGTGA 2 cut(s) 175, 1281
BaeGI GKGCMC 1 cut(s) 1142
BalI TGGCCA 2 cut(s) 251, 630
BbsI GAAGAC 3 cut(s) 395, 923, 936
Bbv12I GWGCWC 1 cut(s) 1142
BbvI GCAGC 4 cut(s) 181, 751, 772, 1057
BccI CCATC 5 cut(s) 7, 598, 728, 736, 1057
BceAI ACGGC 1 cut(s) 1398
BcgI CGANNNNNNTGC 2 cut(s) 767, 801
BciT130I CCWGG 1 cut(s) 1350
BcnI CCSGG 1 cut(s) 1326
BcoDI GTCTC 2 cut(s) 336, 1121
BfaI CTAG 1 cut(s) 1230
BfmI CTRYAG 2 cut(s) 8, 1110
BglI GCCNNNNNGGC 1 cut(s) 257
BisI GCNGC 4 cut(s) 195, 765, 786, 1046
BlsI GCNGC 4 cut(s) 196, 766, 787, 1047
Bme1390I CCNGG 2 cut(s) 1326, 1350
BmrFI CCNGG 2 cut(s) 1326, 1350
BmsI GCATC 2 cut(s) 1004, 1099
BpiI GAAGAC 3 cut(s) 395, 923, 936
BpmI CTGGAG 2 cut(s) 245, 542
BpuMI CCSGG 1 cut(s) 1326
Bsa29I ATCGAT 2 cut(s) 777, 971
BsaBI GATNNNNATC 2 cut(s) 438, 654
BsaJI CCNNGG 5 cut(s) 252, 724, 843, 1325, 1349
BsaWI WCCGGW 2 cut(s) 104, 1291
BsaXI ACNNNNNCTCC 6 cut(s) 218, 248, 515, 545, 1265, 1295
Bsc4I CCNNNNNNNGG 1 cut(s) 160
Bse118I RCCGGY 1 cut(s) 104
Bse1I ACTGG 1 cut(s) 252
Bse3DI GCAATG 2 cut(s) 945, 1180
Bse8I GATNNNNATC 2 cut(s) 438, 654
BseBI CCWGG 1 cut(s) 1350
BseCI ATCGAT 2 cut(s) 777, 971
BseDI CCNNGG 5 cut(s) 252, 724, 843, 1325, 1349
BseGI GGATG 3 cut(s) 40, 720, 1054
BseJI GATNNNNATC 2 cut(s) 438, 654
BseLI CCNNNNNNNGG 1 cut(s) 160
BseMI GCAATG 2 cut(s) 945, 1180
BseMII CTCAG 6 cut(s) 76, 198, 341, 540, 605, 780
BseNI ACTGG 1 cut(s) 252
BseSI GKGCMC 1 cut(s) 1142
BseXI GCAGC 4 cut(s) 181, 751, 772, 1057
BsgI GTGCAG 1 cut(s) 686
Bsh1236I CGCG 1 cut(s) 1399
BshFI GGCC 3 cut(s) 251, 630, 1003
BshTI ACCGGT 1 cut(s) 104
BshVI ATCGAT 2 cut(s) 777, 971
BsiHKAI GWGCWC 1 cut(s) 1142
BsiSI CCGG 3 cut(s) 105, 1292, 1325
BslFI GGGAC 1 cut(s) 785
BslI CCNNNNNNNGG 1 cut(s) 160
BsmAI GTCTC 2 cut(s) 336, 1121
BsmBI CGTCTC 1 cut(s) 1121
BsmFI GGGAC 1 cut(s) 785
BsmI GAATGC 2 cut(s) 196, 1073
BsnI GGCC 3 cut(s) 251, 630, 1003
Bsp1286I GDGCHC 1 cut(s) 1142
Bsp1407I TGTACA 1 cut(s) 1254
Bsp143I GATC 7 cut(s) 340, 433, 504, 552, 649, 968, 1201
Bsp19I CCATGG 1 cut(s) 252
BspACI CCGC 2 cut(s) 464, 671
BspANI GGCC 3 cut(s) 251, 630, 1003
BspCNI CTCAG 6 cut(s) 75, 199, 340, 541, 604, 781
BspDI ATCGAT 2 cut(s) 777, 971
BspFNI CGCG 1 cut(s) 1399
BspMAI CTGCAG 2 cut(s) 12, 1114
BspPI GGATC 4 cut(s) 335, 428, 657, 1196
BspQI GCTCTTC 3 cut(s) 143, 267, 1256
BsrDI GCAATG 2 cut(s) 945, 1180
BsrFI RCCGGY 1 cut(s) 104
BsrGI TGTACA 1 cut(s) 1254
BsrI ACTGG 1 cut(s) 252
BssAI RCCGGY 1 cut(s) 104
BssECI CCNNGG 5 cut(s) 252, 724, 843, 1325, 1349
BssMI GATC 7 cut(s) 340, 433, 504, 552, 649, 968, 1201
BssT1I CCWWGG 2 cut(s) 252, 843
Bst2UI CCWGG 1 cut(s) 1350
Bst4CI ACNGT 2 cut(s) 712, 1342
Bst6I CTCTTC 5 cut(s) 143, 225, 267, 605, 1256
BstAPI GCANNNNNTGC 1 cut(s) 191
BstAUI TGTACA 1 cut(s) 1254
BstC8I GCNNGC 1 cut(s) 1071
BstDSI CCRYGG 1 cut(s) 252
BstF5I GGATG 3 cut(s) 40, 720, 1054
BstFNI CGCG 1 cut(s) 1399
BstHHI GCGC 1 cut(s) 313
BstKTI GATC 7 cut(s) 343, 436, 507, 555, 652, 971, 1204
BstMAI GTCTC 2 cut(s) 336, 1121
BstMBI GATC 7 cut(s) 340, 433, 504, 552, 649, 968, 1201
BstMWI GCNNNNNNNGC 5 cut(s) 191, 257, 266, 636, 1419
BstNI CCWGG 1 cut(s) 1350
BstSCI CCNGG 2 cut(s) 1324, 1348
BstSFI CTRYAG 2 cut(s) 8, 1110
BstSLI GKGCMC 1 cut(s) 1142
BstUI CGCG 1 cut(s) 1399
BstV1I GCAGC 4 cut(s) 181, 751, 772, 1057
BstV2I GAAGAC 3 cut(s) 395, 923, 936
Bsu15I ATCGAT 2 cut(s) 777, 971
BsuRI GGCC 3 cut(s) 251, 630, 1003
BsuTUI ATCGAT 2 cut(s) 777, 971
BtgI CCRYGG 1 cut(s) 252
BtsCI GGATG 3 cut(s) 40, 720, 1054
BtsI GCAGTG 2 cut(s) 1107, 1140
BtsIMutI CAGTG 3 cut(s) 708, 1107, 1140
Cac8I GCNNGC 1 cut(s) 1071
CfoI GCGC 1 cut(s) 313
Cfr10I RCCGGY 1 cut(s) 104
ClaI ATCGAT 2 cut(s) 777, 971
CseI GACGC 2 cut(s) 75, 475
Csp6I GTAC 2 cut(s) 809, 1255
CspAI ACCGGT 1 cut(s) 104
CviAII CATG 6 cut(s) 91, 253, 508, 586, 1258, 1404
CviQI GTAC 2 cut(s) 809, 1255
DpnI GATC 7 cut(s) 342, 435, 506, 554, 651, 970, 1203
DpnII GATC 7 cut(s) 340, 433, 504, 552, 649, 968, 1201
EaeI YGGCCR 2 cut(s) 249, 628
Eam1104I CTCTTC 5 cut(s) 143, 225, 267, 605, 1256
EarI CTCTTC 5 cut(s) 143, 225, 267, 605, 1256
Eco130I CCWWGG 2 cut(s) 252, 843
Eco57I CTGAAG 3 cut(s) 30, 407, 1227
EcoRII CCWGG 1 cut(s) 1348
EcoT14I CCWWGG 2 cut(s) 252, 843
ErhI CCWWGG 2 cut(s) 252, 843
Esp3I CGTCTC 1 cut(s) 1121
FaeI CATG 6 cut(s) 94, 256, 511, 589, 1261, 1407
FaqI GGGAC 1 cut(s) 785
FatI CATG 6 cut(s) 90, 252, 507, 585, 1257, 1403
FblI GTMKAC 1 cut(s) 1389
Fnu4HI GCNGC 4 cut(s) 195, 765, 786, 1046
FokI GGATG 3 cut(s) 47, 707, 1041
Fsp4HI GCNGC 4 cut(s) 195, 765, 786, 1046
FspBI CTAG 1 cut(s) 1230
FspI TGCGCA 1 cut(s) 312
GlaI GCGC 1 cut(s) 312
GluI GCNGC 4 cut(s) 195, 765, 786, 1046
GsuI CTGGAG 2 cut(s) 245, 542
HaeIII GGCC 3 cut(s) 251, 630, 1003
HapII CCGG 3 cut(s) 105, 1292, 1325
HgaI GACGC 2 cut(s) 75, 475
HhaI GCGC 1 cut(s) 313
Hin1II CATG 6 cut(s) 94, 256, 511, 589, 1261, 1407
Hin6I GCGC 1 cut(s) 311
HinP1I GCGC 1 cut(s) 311
HincII GTYRAC 1 cut(s) 1409
HindII GTYRAC 1 cut(s) 1409
HindIII AAGCTT 2 cut(s) 876, 1209
HpaII CCGG 3 cut(s) 105, 1292, 1325
HphI GGTGA 2 cut(s) 175, 1281
Hpy166II GTNNAC 5 cut(s) 1140, 1255, 1338, 1390, 1409
Hpy8I GTNNAC 5 cut(s) 1140, 1255, 1338, 1390, 1409
Hpy99I CGWCG 2 cut(s) 1315, 1375
HpyAV CCTTC 2 cut(s) 113, 1207
HpyCH4III ACNGT 2 cut(s) 712, 1342
HpyF10VI GCNNNNNNNGC 5 cut(s) 191, 257, 266, 636, 1419
Hsp92II CATG 6 cut(s) 94, 256, 511, 589, 1261, 1407
HspAI GCGC 1 cut(s) 311
Kzo9I GATC 7 cut(s) 340, 433, 504, 552, 649, 968, 1201
LguI GCTCTTC 3 cut(s) 143, 267, 1256
LmnI GCTCC 3 cut(s) 847, 1066, 1294
Lsp1109I GCAGC 4 cut(s) 181, 751, 772, 1057
LweI GCATC 2 cut(s) 1004, 1099
MaeI CTAG 1 cut(s) 1230
MaeIII GTNAC 3 cut(s) 122, 691, 1105
MalI GATC 7 cut(s) 342, 435, 506, 554, 651, 970, 1203
MboI GATC 7 cut(s) 340, 433, 504, 552, 649, 968, 1201
MhlI GDGCHC 1 cut(s) 1142
MlsI TGGCCA 2 cut(s) 251, 630
MluCI AATT 2 cut(s) 795, 1359
MluNI TGGCCA 2 cut(s) 251, 630
MlyI GAGTC 4 cut(s) 26, 218, 539, 583
MmeI TCCRAC 3 cut(s) 150, 1251, 1293
MnlI CCTC 7 cut(s) 220, 250, 719, 1226, 1267, 1378, 1403
Mox20I TGGCCA 2 cut(s) 251, 630
MroXI GAANNNNTTC 2 cut(s) 573, 879
MscI TGGCCA 2 cut(s) 251, 630
MseI TTAA 4 cut(s) 491, 533, 617, 794
MslI CAYNNNNRTG 2 cut(s) 89, 779
Msp20I TGGCCA 2 cut(s) 251, 630
MspI CCGG 3 cut(s) 105, 1292, 1325
MspR9I CCNGG 2 cut(s) 1326, 1350
Mva1269I GAATGC 2 cut(s) 196, 1073
MvaI CCWGG 1 cut(s) 1350
MvnI CGCG 1 cut(s) 1399
MwoI GCNNNNNNNGC 5 cut(s) 191, 257, 266, 636, 1419
NciI CCSGG 1 cut(s) 1326
NcoI CCATGG 1 cut(s) 252
NdeII GATC 7 cut(s) 340, 433, 504, 552, 649, 968, 1201
NlaIII CATG 6 cut(s) 94, 256, 511, 589, 1261, 1407
NmeAIII GCCGAG 1 cut(s) 979
NmuCI GTSAC 1 cut(s) 1105
NsbI TGCGCA 1 cut(s) 312
PciSI GCTCTTC 3 cut(s) 143, 267, 1256
PcsI WCGNNNNNNNCGW 3 cut(s) 290, 917, 1277
PctI GAATGC 2 cut(s) 196, 1073
PdmI GAANNNNTTC 2 cut(s) 573, 879
PfeI GAWTC 8 cut(s) 4, 352, 469, 581, 729, 905, 954, 1242
PinAI ACCGGT 1 cut(s) 104
PkrI GCNGC 4 cut(s) 196, 766, 787, 1047
PleI GAGTC 4 cut(s) 25, 217, 539, 583
PpsI GAGTC 4 cut(s) 25, 217, 539, 583
Psp6I CCWGG 1 cut(s) 1348
PspGI CCWGG 1 cut(s) 1348
PstI CTGCAG 2 cut(s) 12, 1114
RsaI GTAC 2 cut(s) 810, 1256
RsaNI GTAC 2 cut(s) 809, 1255
RseI CAYNNNNRTG 2 cut(s) 89, 779
SapI GCTCTTC 3 cut(s) 143, 267, 1256
SaqAI TTAA 4 cut(s) 491, 533, 617, 794
SatI GCNGC 4 cut(s) 195, 765, 786, 1046
Sau3AI GATC 7 cut(s) 340, 433, 504, 552, 649, 968, 1201
SchI GAGTC 4 cut(s) 26, 218, 539, 583
ScrFI CCNGG 2 cut(s) 1326, 1350
SduI GDGCHC 1 cut(s) 1142
SfaNI GCATC 2 cut(s) 1004, 1099
SfcI CTRYAG 2 cut(s) 8, 1110
SmiMI CAYNNNNRTG 2 cut(s) 89, 779
Sse9I AATT 2 cut(s) 795, 1359
SsiI CCGC 2 cut(s) 464, 671
SspMI CTAG 1 cut(s) 1230
StyD4I CCNGG 2 cut(s) 1324, 1348
StyI CCWWGG 2 cut(s) 252, 843
TaaI ACNGT 2 cut(s) 712, 1342
TaqI TCGA 7 cut(s) 293, 680, 777, 911, 971, 1152, 1200
TasI AATT 2 cut(s) 795, 1359
TatI WGTACW 2 cut(s) 808, 1254
TfiI GAWTC 8 cut(s) 4, 352, 469, 581, 729, 905, 954, 1242
Tru1I TTAA 4 cut(s) 491, 533, 617, 794
Tru9I TTAA 4 cut(s) 491, 533, 617, 794
TscAI CASTG 3 cut(s) 715, 1114, 1147
TseFI GTSAC 1 cut(s) 1105
TseI GCWGC 4 cut(s) 194, 764, 785, 1045
Tsp45I GTSAC 1 cut(s) 1105
TspDTI ATGAA 7 cut(s) 17, 107, 650, 770, 989, 1274, 1392
TspRI CASTG 3 cut(s) 715, 1114, 1147
VneI GTGCAC 1 cut(s) 1138
XbaI TCTAGA 1 cut(s) 1229
XmiI GTMKAC 1 cut(s) 1389
XmnI GAANNNNTTC 2 cut(s) 573, 879
XspI CTAG 1 cut(s) 1230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.