Rorug05G0118700

Plant protein of unknown function (DUF641)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0004482
Physical Location & Seq
Reverse (-)
10490445 .. 10492263
1819 bp
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UTR
Exon/CDS
Intron
Rorug05G0118700.1

Sequence Viewer

Length: 687 bp
ATGAGGAACACCACCAGCACCGGATCATCAGCGAACTCGTGCAGAAGAAGACCCGAACCCAAAGGACACAACCAAAGGATACAGATCAACAACACAACAAGCCACCTACCACCTACCACTGCCGGGACGTCCCTCATATTGCCGCCGCCATCGCTGCAATGCCACCGTCAATCTCCATGCCTCAGCACAGCAATGAACCTGAATCCAGCGCTGCTGCCGTCAAACTCCAAGCCAAAAGCCAAACCCGAAAGGCCACAATCCAAGTCCGTCGACGACCTTAACTCCCTCGCCTACCATCTCATCACTTCCGTTCTAGCCGATGGATCGGCCAAAGGCGGGCCATGTTTGAACTTGGCCAACGGTTTATGGACCGACGAGTCTAAACCTCTTGAGGATTCTTACAAAGAGGTGGTGTGCGGGTCTTACGAGGCTTCCCTAAAGCAAGTCGATTTCAAGGTCAACCCCGGTAAAGTGAGAGTTGAAGTTAATTCATGGGTGAAGAAGGAGACAAAAGGCCATATCACTGAGATTCTTCCTCTAAACTCAGTCACCAACGAAACAAGACGCATCTTTGCAAATGCTTTATACTTCAATGCCTCTTGGAGAGAGAGCTACCGCTTCCATGAACCCTACACAAAAGAGAAGGATCATGAGTTCCACCTTCTCAATGGGGACTCGGTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF641

Protein Analysis

228

Amino Acids

25.34

Weight (kDa)

9.18

Isoelectric Point (pI)

44.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Serpin PF00079 90 - 228 2.4e-23 Serpin (serine protease inhibitor)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 376
AatII GACGTC 1 cut(s) 131
AccI GTMKAC 1 cut(s) 270
AciI CCGC 5 cut(s) 143, 146, 336, 417, 616
AclWI GGATC 3 cut(s) 31, 331, 654
AcoI YGGCCR 2 cut(s) 327, 354
AcyI GRCGYC 1 cut(s) 128
AfeI AGCGCT 1 cut(s) 210
AfiI CCNNNNNNNGG 2 cut(s) 123, 336
AgsI TTSAA 4 cut(s) 349, 454, 482, 592
AloI GAACNNNNNNTCC 2 cut(s) 638, 670
AluBI AGCT 1 cut(s) 612
AluI AGCT 1 cut(s) 612
Alw26I GTCTC 1 cut(s) 500
AlwI GGATC 3 cut(s) 31, 331, 654
Aor51HI AGCGCT 1 cut(s) 210
AoxI GGCC 5 cut(s) 251, 327, 338, 354, 514
ApeKI GCWGC 3 cut(s) 154, 211, 214
AspLEI GCGC 1 cut(s) 211
AspS9I GGNCC 2 cut(s) 338, 369
AsuC2I CCSGG 2 cut(s) 124, 465
AsuHPI GGTGA 2 cut(s) 508, 541
AvaII GGWCC 1 cut(s) 369
BalI TGGCCA 1 cut(s) 356
BauI CACGAG 1 cut(s) 37
BbsI GAAGAC 1 cut(s) 55
BbvCI CCTCAGC 1 cut(s) 182
BbvI GCAGC 3 cut(s) 141, 198, 201
BccI CCATC 3 cut(s) 157, 303, 314
BceAI ACGGC 1 cut(s) 202
BciVI GTATCC 1 cut(s) 72
BcnI CCSGG 2 cut(s) 124, 465
BcoDI GTCTC 1 cut(s) 500
BfaI CTAG 1 cut(s) 314
BfoI RGCGCY 1 cut(s) 212
BfuI GTATCC 1 cut(s) 72
BisI GCNGC 5 cut(s) 143, 146, 155, 212, 215
BlsI GCNGC 5 cut(s) 144, 147, 156, 213, 216
Bme1390I CCNGG 2 cut(s) 124, 465
Bme18I GGWCC 1 cut(s) 369
BmgT120I GGNCC 2 cut(s) 338, 369
BmrFI CCNGG 2 cut(s) 124, 465
BmsI GCATC 1 cut(s) 576
BpiI GAAGAC 1 cut(s) 55
Bpu10I CCTNAGC 1 cut(s) 182
BpuEI CTTGAG 1 cut(s) 410
BpuMI CCSGG 2 cut(s) 124, 465
BsaBI GATNNNNATC 1 cut(s) 83
BsaHI GRCGYC 1 cut(s) 128
BsaJI CCNNGG 1 cut(s) 463
BsaWI WCCGGW 1 cut(s) 20
BsaXI ACNNNNNCTCC 2 cut(s) 266, 296
Bsc4I CCNNNNNNNGG 2 cut(s) 123, 336
Bse3DI GCAATG 2 cut(s) 164, 198
Bse8I GATNNNNATC 1 cut(s) 83
BseDI CCNNGG 1 cut(s) 463
BseJI GATNNNNATC 1 cut(s) 83
BseLI CCNNNNNNNGG 2 cut(s) 123, 336
BseMI GCAATG 2 cut(s) 164, 198
BseMII CTCAG 3 cut(s) 196, 516, 558
BseXI GCAGC 3 cut(s) 141, 198, 201
BsgI GTGCAG 1 cut(s) 61
BshFI GGCC 5 cut(s) 253, 329, 340, 356, 516
BsiSI CCGG 3 cut(s) 21, 123, 465
BslFI GGGAC 2 cut(s) 115, 139
BslI CCNNNNNNNGG 2 cut(s) 123, 336
BsmAI GTCTC 1 cut(s) 500
BsmFI GGGAC 2 cut(s) 115, 139
BsnI GGCC 5 cut(s) 253, 329, 340, 356, 516
Bsp143I GATC 4 cut(s) 23, 84, 323, 646
BspACI CCGC 5 cut(s) 143, 146, 336, 417, 616
BspANI GGCC 5 cut(s) 253, 329, 340, 356, 516
BspCNI CTCAG 3 cut(s) 195, 517, 557
BspHI TCATGA 1 cut(s) 649
BspPI GGATC 3 cut(s) 31, 331, 654
BsrDI GCAATG 2 cut(s) 164, 198
BssECI CCNNGG 1 cut(s) 463
BssMI GATC 4 cut(s) 23, 84, 323, 646
BssNI GRCGYC 1 cut(s) 128
BssSI CACGAG 1 cut(s) 37
Bst2BI CACGAG 1 cut(s) 37
Bst4CI ACNGT 2 cut(s) 167, 362
BstACI GRCGYC 1 cut(s) 128
BstC8I GCNNGC 1 cut(s) 338
BstDEI CTNAG 3 cut(s) 182, 525, 544
BstH2I RGCGCY 1 cut(s) 212
BstHHI GCGC 1 cut(s) 211
BstKTI GATC 4 cut(s) 26, 87, 326, 649
BstMAI GTCTC 1 cut(s) 500
BstMBI GATC 4 cut(s) 23, 84, 323, 646
BstMWI GCNNNNNNNGC 2 cut(s) 151, 154
BstSCI CCNGG 2 cut(s) 122, 463
BstV1I GCAGC 3 cut(s) 141, 198, 201
BstV2I GAAGAC 1 cut(s) 55
BsuI GTATCC 1 cut(s) 72
BsuRI GGCC 5 cut(s) 253, 329, 340, 356, 516
BtgZI GCGATG 1 cut(s) 135
BtsI GCAGTG 1 cut(s) 117
BtsIMutI CAGTG 2 cut(s) 117, 522
Cac8I GCNNGC 1 cut(s) 338
CciI TCATGA 1 cut(s) 649
CfoI GCGC 1 cut(s) 211
Cfr13I GGNCC 2 cut(s) 338, 369
CseI GACGC 1 cut(s) 573
CviAII CATG 5 cut(s) 177, 342, 492, 623, 650
DdeI CTNAG 3 cut(s) 182, 525, 544
DpnI GATC 4 cut(s) 25, 86, 325, 648
DpnII GATC 4 cut(s) 23, 84, 323, 646
DrdI GACNNNNNNGTC 1 cut(s) 376
DseDI GACNNNNNNGTC 1 cut(s) 376
EaeI YGGCCR 2 cut(s) 327, 354
Eco47I GGWCC 1 cut(s) 369
Eco47III AGCGCT 1 cut(s) 210
FaeI CATG 5 cut(s) 180, 345, 495, 626, 653
FaiI YATR 9 cut(s) 137, 178, 343, 367, 493, 519, 586, 624, 651
FaqI GGGAC 2 cut(s) 115, 139
FatI CATG 5 cut(s) 176, 341, 491, 622, 649
FauI CCCGC 2 cut(s) 329, 410
FblI GTMKAC 1 cut(s) 270
Fnu4HI GCNGC 5 cut(s) 143, 146, 155, 212, 215
Fsp4HI GCNGC 5 cut(s) 143, 146, 155, 212, 215
FspBI CTAG 1 cut(s) 314
GlaI GCGC 1 cut(s) 210
GluI GCNGC 5 cut(s) 143, 146, 155, 212, 215
HaeII RGCGCY 1 cut(s) 212
HaeIII GGCC 5 cut(s) 253, 329, 340, 356, 516
HapII CCGG 3 cut(s) 21, 123, 465
HgaI GACGC 1 cut(s) 573
HhaI GCGC 1 cut(s) 211
Hin1I GRCGYC 1 cut(s) 128
Hin1II CATG 5 cut(s) 180, 345, 495, 626, 653
Hin6I GCGC 1 cut(s) 209
HinP1I GCGC 1 cut(s) 209
HincII GTYRAC 2 cut(s) 271, 460
HindII GTYRAC 2 cut(s) 271, 460
HinfI GANTC 5 cut(s) 202, 377, 395, 529, 674
HpaII CCGG 3 cut(s) 21, 123, 465
HphI GGTGA 2 cut(s) 508, 541
Hpy166II GTNNAC 2 cut(s) 271, 460
Hpy188III TCNNGA 2 cut(s) 389, 650
Hpy8I GTNNAC 2 cut(s) 271, 460
Hpy99I CGWCG 3 cut(s) 272, 275, 377
HpyAV CCTTC 3 cut(s) 496, 637, 671
HpyCH4III ACNGT 2 cut(s) 167, 362
HpyCH4IV ACGT 1 cut(s) 128
HpyCH4V TGCA 3 cut(s) 42, 157, 575
HpyF10VI GCNNNNNNNGC 2 cut(s) 151, 154
HpyF3I CTNAG 3 cut(s) 182, 525, 544
HpySE526I ACGT 1 cut(s) 128
Hsp92I GRCGYC 1 cut(s) 128
Hsp92II CATG 5 cut(s) 180, 345, 495, 626, 653
HspAI GCGC 1 cut(s) 209
Kzo9I GATC 4 cut(s) 23, 84, 323, 646
LpnPI CCDG 6 cut(s) 28, 34, 136, 212, 219, 478
Lsp1109I GCAGC 3 cut(s) 141, 198, 201
LweI GCATC 1 cut(s) 576
MaeI CTAG 1 cut(s) 314
MaeII ACGT 1 cut(s) 128
MaeIII GTNAC 1 cut(s) 547
MalI GATC 4 cut(s) 25, 86, 325, 648
MboI GATC 4 cut(s) 23, 84, 323, 646
MboII GAAGA 4 cut(s) 57, 60, 511, 524
MlsI TGGCCA 1 cut(s) 356
MluCI AATT 1 cut(s) 487
MluNI TGGCCA 1 cut(s) 356
MlyI GAGTC 2 cut(s) 386, 668
MnlI CCTC 9 cut(s) 143, 191, 296, 385, 396, 400, 421, 546, 607
Mox20I TGGCCA 1 cut(s) 356
MscI TGGCCA 1 cut(s) 356
MseI TTAA 2 cut(s) 279, 486
MslI CAYNNNNRTG 1 cut(s) 191
Msp20I TGGCCA 1 cut(s) 356
MspI CCGG 3 cut(s) 21, 123, 465
MspR9I CCNGG 2 cut(s) 124, 465
MwoI GCNNNNNNNGC 2 cut(s) 151, 154
NciI CCSGG 2 cut(s) 124, 465
NdeII GATC 4 cut(s) 23, 84, 323, 646
NlaIII CATG 5 cut(s) 180, 345, 495, 626, 653
NmuCI GTSAC 1 cut(s) 547
PagI TCATGA 1 cut(s) 649
PfeI GAWTC 3 cut(s) 202, 395, 529
PflFI GACNNNGTC 1 cut(s) 677
PkrI GCNGC 5 cut(s) 144, 147, 156, 213, 216
PleI GAGTC 2 cut(s) 385, 668
PpsI GAGTC 2 cut(s) 385, 668
PspPI GGNCC 2 cut(s) 338, 369
PsyI GACNNNGTC 1 cut(s) 677
RseI CAYNNNNRTG 1 cut(s) 191
SalI GTCGAC 1 cut(s) 269
SaqAI TTAA 2 cut(s) 279, 486
SatI GCNGC 5 cut(s) 143, 146, 155, 212, 215
Sau3AI GATC 4 cut(s) 23, 84, 323, 646
Sau96I GGNCC 2 cut(s) 338, 369
SchI GAGTC 2 cut(s) 386, 668
ScrFI CCNGG 2 cut(s) 124, 465
SfaNI GCATC 1 cut(s) 576
SgrDI CGTCGACG 1 cut(s) 269
SinI GGWCC 1 cut(s) 369
SmiMI CAYNNNNRTG 1 cut(s) 191
SmlI CTYRAG 1 cut(s) 389
SmoI CTYRAG 1 cut(s) 389
Sse9I AATT 1 cut(s) 487
SsiI CCGC 5 cut(s) 143, 146, 336, 417, 616
SspMI CTAG 1 cut(s) 314
StyD4I CCNGG 2 cut(s) 122, 463
TaaI ACNGT 2 cut(s) 167, 362
TaiI ACGT 1 cut(s) 131
TaqI TCGA 2 cut(s) 270, 447
TaqII GACCGA 2 cut(s) 386, 667
TasI AATT 1 cut(s) 487
TauI GCSGC 2 cut(s) 145, 148
TfiI GAWTC 3 cut(s) 202, 395, 529
Tru1I TTAA 2 cut(s) 279, 486
Tru9I TTAA 2 cut(s) 279, 486
TscAI CASTG 2 cut(s) 124, 529
TseFI GTSAC 1 cut(s) 547
TseI GCWGC 3 cut(s) 154, 211, 214
Tsp45I GTSAC 1 cut(s) 547
TspDTI ATGAA 3 cut(s) 209, 480, 639
TspGWI ACGGA 2 cut(s) 256, 298
TspRI CASTG 2 cut(s) 124, 529
Tth111I GACNNNGTC 1 cut(s) 677
VpaK11BI GGWCC 1 cut(s) 369
XcmI CCANNNNNNNNNTGG 1 cut(s) 665
XmiI GTMKAC 1 cut(s) 270
XspI CTAG 1 cut(s) 314
ZraI GACGTC 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.