AT3G15090

Zinc-binding dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0012404
Physical Location & Seq
Forward (+)
5076624 .. 5079159
2536 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G15090.1

Sequence Viewer

Length: 1101 bp
ATGCGAGTGATGAGATCATTGCGCGGTAATTCCGGTGCTGGATTGGTTTTCCGGCCGGCGAGGTTAAATTCTCTTCGGAGCGTCTTTACCGGTTGCCGTGCTGTGATTTTGCCCCGCTTTGGTGGTCCGGAGGTTTTTGAGCTCCGGGAGAATGTTCCGGTGCCGAATCTGAATCCAAATGAGGTTCTTGTCAAGGCGAAAGCTGTCTCCGTTAATCCTCTTGATTGCAGAATACGAGCTGGATATGGACGTTCTGTATTCCAACCGCATCTACCTATTATAGTTGGACGTGATGTCAGTGGTGAAGTTGCGGCAATTGGGACTTCAGTAAAGTCACTTAAAGTAGGACAAGAAGTTTTTGGTGCGTTGCATCCGACGGCGTTAAGAGGTACTTATACTGACTATGGAATTCTTTCGGAAGACGAACTCACGGAAAAGCCATCATCAATTTCACATGTGGAAGCAAGTGCCATTCCTTTTGCAGCTTTGACTGCTTGGCGTGCTTTGAAGAGTAATGCAAGGATAACTGAAGGACAAAGACTATTAGTCTTTGGAGGAGGAGGAGCAGTAGGTTTTTCTGCAATCCAGCTTGCGGTAGCATCTGGGTGTCATGTTACAGCCTCTTGTGTTGGTCAGACTAAAGACAGAATACTTGCAGCTGGGGCCGAGCAAGCTGTTGACTACACAACCGAGGACATTGAGTTGGCGGTAAAAGGAAAATTTGATGCCGTGTTAGATACTATTGGTGGGCCTGAAACGGAGAGAATAGGCATTAACTTCTTAAGGAAGGGTGGAAACTATATGACTCTTCAGGGTGAGGCTGCATCGTTGACTGATAAATACGGTTTTGTGGTTGGGCTTCCCCTTGCAACATCGCTCTTGATGAAGAAAAAGATACAATATCAGTATTCTCATGGAATAGACTATTGGTGGACGTATATGAGGGCTGATCCTGAAGGTCTAGCCGAGATTCAGCGTTTAGTTGGAGCTGGAAAGCTAAAAATACCTGTTGAAAAAACATTTCCAATAACCGATGTTGTAGCAGCTCATGAAGCCAAGGAGAAGAAACAGATTCCGGGTAAGGTGGTTCTAGAGTTCTGA

Protein Analysis

366

Amino Acids

39.39

Weight (kDa)

9.13

Isoelectric Point (pI)

25.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 59 - 146 6.4e-14 Alcohol dehydrogenase GroES-like domain
ADH_zinc_N PF00107 189 - 272 3.7e-10 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 221 - 364 2.9e-20 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012404)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15090
fragaria_vesca FvH4_3g19130
malus_domestica MD03G1236200.v1.1 MD11G1257100.v1.1
prunus_persica Prupe.4G173000_v2.0.a1
pyrus_communis pycom03g18240 pycom11g22720
rosa_chinensis RchiOBHm_Chr5g0031931 RchiOBHm_Chr5g0032001 RchiOBHm_Chr5g0032041
rosa_laevigata RLG00000033359
rosa_multiflora Rmu_sc0003031.1_g000010 Rmu_sc0005375.1_g000023
rosa_roxburghii Rroxscaffold_1G00048330
rosa_rugosa Rorug05G0129400
rosa_samantha Rh5CG246600 Rh5DG228300
rosa_wichuraiana Rw5G020340 Rw5G020360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 160
AccII CGCG 1 cut(s) 24
AccIII TCCGGA 1 cut(s) 127
AciI CCGC 6 cut(s) 24, 115, 266, 311, 593, 707
AclWI GGATC 1 cut(s) 944
AcoI YGGCCR 1 cut(s) 53
AcsI RAATTY 3 cut(s) 67, 408, 719
AcuI CTGAAG 4 cut(s) 309, 549, 794, 975
AfaI GTAC 1 cut(s) 391
AfiI CCNNNNNNNGG 2 cut(s) 119, 592
AflII CTTAAG 1 cut(s) 781
AflIII ACRYGT 1 cut(s) 454
AgeI ACCGGT 1 cut(s) 89
AgsI TTSAA 2 cut(s) 508, 1013
AhdI GACNNNNNGTC 2 cut(s) 293, 545
AjiI CACGTC 1 cut(s) 290
AjuI GAANNNNNNNTTGG 2 cut(s) 910, 942
Alw21I GWGCWC 1 cut(s) 144
Alw26I GTCTC 1 cut(s) 211
AlwI GGATC 1 cut(s) 944
Aor13HI TCCGGA 1 cut(s) 127
AoxI GGCC 3 cut(s) 53, 663, 749
ApeKI GCWGC 4 cut(s) 482, 656, 821, 1043
ApoI RAATTY 3 cut(s) 67, 408, 719
AsiGI ACCGGT 1 cut(s) 89
Asp700I GAANNNNTTC 1 cut(s) 412
AspLEI GCGC 1 cut(s) 24
AspS9I GGNCC 3 cut(s) 125, 663, 749
AsuC2I CCSGG 2 cut(s) 146, 1077
AsuHPI GGTGA 2 cut(s) 314, 827
AvaII GGWCC 1 cut(s) 125
BanI GGYRCC 1 cut(s) 160
BanII GRGCYC 1 cut(s) 144
BbsI GAAGAC 1 cut(s) 426
Bbv12I GWGCWC 1 cut(s) 144
BbvI GCAGC 4 cut(s) 494, 668, 808, 1055
BccI CCATC 1 cut(s) 448
BceAI ACGGC 3 cut(s) 81, 393, 713
BcnI CCSGG 2 cut(s) 146, 1077
BcoDI GTCTC 1 cut(s) 211
BfaI CTAG 2 cut(s) 962, 1091
BfrI CTTAAG 1 cut(s) 781
BisI GCNGC 5 cut(s) 312, 483, 657, 822, 1044
BlsI GCNGC 5 cut(s) 313, 484, 658, 823, 1045
Bme1390I CCNGG 2 cut(s) 146, 1077
Bme18I GGWCC 1 cut(s) 125
BmeRI GACNNNNNGTC 2 cut(s) 293, 545
BmgBI CACGTC 1 cut(s) 290
BmgT120I GGNCC 3 cut(s) 125, 663, 749
BmiI GGNNCC 2 cut(s) 162, 664
BmrFI CCNGG 2 cut(s) 146, 1077
BmsI GCATC 5 cut(s) 277, 379, 608, 715, 833
BpiI GAAGAC 1 cut(s) 426
BpuMI CCSGG 2 cut(s) 146, 1077
BsaJI CCNNGG 2 cut(s) 690, 1056
BsaWI WCCGGW 4 cut(s) 32, 89, 127, 157
BsaXI ACNNNNNCTCC 2 cut(s) 552, 582
Bsc4I CCNNNNNNNGG 2 cut(s) 119, 592
Bse118I RCCGGY 2 cut(s) 55, 89
Bse3DI GCAATG 1 cut(s) 17
BseAI TCCGGA 1 cut(s) 127
BseDI CCNNGG 2 cut(s) 690, 1056
BseGI GGATG 1 cut(s) 370
BseLI CCNNNNNNNGG 2 cut(s) 119, 592
BseMI GCAATG 1 cut(s) 17
BseRI GAGGAG 3 cut(s) 570, 573, 576
BseX3I CGGCCG 1 cut(s) 53
BseXI GCAGC 4 cut(s) 494, 668, 808, 1055
BseYI CCCAGC 1 cut(s) 659
Bsh1236I CGCG 1 cut(s) 24
Bsh1285I CGRYCG 1 cut(s) 56
BshFI GGCC 3 cut(s) 55, 665, 751
BshNI GGYRCC 1 cut(s) 160
BshTI ACCGGT 1 cut(s) 89
BsiEI CGRYCG 1 cut(s) 56
BsiHKAI GWGCWC 1 cut(s) 144
BsiSI CCGG 8 cut(s) 33, 52, 56, 90, 128, 145, 158, 1076
BslFI GGGAC 1 cut(s) 334
BslI CCNNNNNNNGG 2 cut(s) 119, 592
BsmAI GTCTC 1 cut(s) 211
BsmFI GGGAC 1 cut(s) 334
BsnI GGCC 3 cut(s) 55, 665, 751
Bsp1286I GDGCHC 1 cut(s) 144
Bsp13I TCCGGA 1 cut(s) 127
Bsp143I GATC 2 cut(s) 14, 949
BspACI CCGC 6 cut(s) 24, 115, 266, 311, 593, 707
BspANI GGCC 3 cut(s) 55, 665, 751
BspEI TCCGGA 1 cut(s) 127
BspFNI CGCG 1 cut(s) 24
BspHI TCATGA 1 cut(s) 1048
BspLI GGNNCC 2 cut(s) 162, 664
BspPI GGATC 1 cut(s) 944
BspT107I GGYRCC 1 cut(s) 160
BspTI CTTAAG 1 cut(s) 781
BsrDI GCAATG 1 cut(s) 17
BsrFI RCCGGY 2 cut(s) 55, 89
BssAI RCCGGY 2 cut(s) 55, 89
BssECI CCNNGG 2 cut(s) 690, 1056
BssMI GATC 2 cut(s) 14, 949
BssT1I CCWWGG 1 cut(s) 1056
Bst4CI ACNGT 1 cut(s) 845
Bst6I CTCTTC 3 cut(s) 78, 503, 813
BstAFI CTTAAG 1 cut(s) 781
BstC8I GCNNGC 4 cut(s) 57, 501, 591, 672
BstF5I GGATG 1 cut(s) 370
BstFNI CGCG 1 cut(s) 24
BstHHI GCGC 1 cut(s) 24
BstKTI GATC 2 cut(s) 17, 952
BstMAI GTCTC 1 cut(s) 211
BstMBI GATC 2 cut(s) 14, 949
BstMCI CGRYCG 1 cut(s) 56
BstMWI GCNNNNNNNGC 5 cut(s) 491, 500, 662, 671, 1052
BstNSI RCATGY 1 cut(s) 458
BstSCI CCNGG 2 cut(s) 144, 1075
BstUI CGCG 1 cut(s) 24
BstV1I GCAGC 4 cut(s) 494, 668, 808, 1055
BstV2I GAAGAC 1 cut(s) 426
BstZI CGGCCG 1 cut(s) 53
BsuRI GGCC 3 cut(s) 55, 665, 751
BtgZI GCGATG 1 cut(s) 858
BtrI CACGTC 1 cut(s) 290
BtsCI GGATG 1 cut(s) 370
BtsIMutI CAGTG 1 cut(s) 304
Cac8I GCNNGC 4 cut(s) 57, 501, 591, 672
CciI TCATGA 1 cut(s) 1048
CfoI GCGC 1 cut(s) 24
Cfr10I RCCGGY 2 cut(s) 55, 89
Cfr13I GGNCC 3 cut(s) 125, 663, 749
CseI GACGC 1 cut(s) 70
Csp6I GTAC 1 cut(s) 390
CspAI ACCGGT 1 cut(s) 89
CviAII CATG 4 cut(s) 455, 611, 914, 1049
CviQI GTAC 1 cut(s) 390
DpnI GATC 2 cut(s) 16, 951
DpnII GATC 2 cut(s) 14, 949
DriI GACNNNNNGTC 2 cut(s) 293, 545
EaeI YGGCCR 1 cut(s) 53
EagI CGGCCG 1 cut(s) 53
Eam1104I CTCTTC 3 cut(s) 78, 503, 813
Eam1105I GACNNNNNGTC 2 cut(s) 293, 545
EarI CTCTTC 3 cut(s) 78, 503, 813
Ecl136II GAGCTC 1 cut(s) 142
EclXI CGGCCG 1 cut(s) 53
Eco130I CCWWGG 1 cut(s) 1056
Eco24I GRGCYC 1 cut(s) 144
Eco47I GGWCC 1 cut(s) 125
Eco52I CGGCCG 1 cut(s) 53
Eco53kI GAGCTC 1 cut(s) 142
Eco57I CTGAAG 4 cut(s) 309, 549, 794, 975
EcoICRI GAGCTC 1 cut(s) 142
EcoRI GAATTC 1 cut(s) 408
EcoT14I CCWWGG 1 cut(s) 1056
EcoT38I GRGCYC 1 cut(s) 144
ErhI CCWWGG 1 cut(s) 1056
FaeI CATG 4 cut(s) 458, 614, 917, 1052
FalI AAGNNNNNCTT 2 cut(s) 376, 408
FaqI GGGAC 1 cut(s) 334
FatI CATG 4 cut(s) 454, 610, 913, 1048
FauI CCCGC 1 cut(s) 122
Fnu4HI GCNGC 5 cut(s) 312, 483, 657, 822, 1044
FokI GGATG 1 cut(s) 357
FriOI GRGCYC 1 cut(s) 144
Fsp4HI GCNGC 5 cut(s) 312, 483, 657, 822, 1044
FspBI CTAG 2 cut(s) 962, 1091
GlaI GCGC 1 cut(s) 23
GluI GCNGC 5 cut(s) 312, 483, 657, 822, 1044
GsaI CCCAGC 1 cut(s) 663
HaeIII GGCC 3 cut(s) 55, 665, 751
HapII CCGG 8 cut(s) 33, 52, 56, 90, 128, 145, 158, 1076
HgaI GACGC 1 cut(s) 70
HhaI GCGC 1 cut(s) 24
Hin1II CATG 4 cut(s) 458, 614, 917, 1052
Hin6I GCGC 1 cut(s) 22
HinP1I GCGC 1 cut(s) 22
HincII GTYRAC 2 cut(s) 679, 831
HindII GTYRAC 2 cut(s) 679, 831
HinfI GANTC 5 cut(s) 166, 172, 805, 970, 1072
HpaII CCGG 8 cut(s) 33, 52, 56, 90, 128, 145, 158, 1076
HphI GGTGA 2 cut(s) 314, 827
Hpy166II GTNNAC 3 cut(s) 679, 831, 933
Hpy188I TCNGA 6 cut(s) 78, 171, 375, 418, 636, 1100
Hpy188III TCNNGA 6 cut(s) 128, 221, 880, 953, 1049, 1091
Hpy8I GTNNAC 3 cut(s) 679, 831, 933
Hpy99I CGWCG 1 cut(s) 379
HpyAV CCTTC 3 cut(s) 524, 781, 950
HpyCH4III ACNGT 1 cut(s) 845
HpyCH4IV ACGT 3 cut(s) 250, 289, 935
HpyCH4V TGCA 8 cut(s) 228, 370, 482, 518, 581, 656, 824, 869
HpyF10VI GCNNNNNNNGC 5 cut(s) 491, 500, 662, 671, 1052
HpySE526I ACGT 3 cut(s) 250, 289, 935
Hsp92II CATG 4 cut(s) 458, 614, 917, 1052
HspAI GCGC 1 cut(s) 22
Kpn2I TCCGGA 1 cut(s) 127
KroI GCCGGC 1 cut(s) 55
KroNI GCCGGC 1 cut(s) 57
Kzo9I GATC 2 cut(s) 14, 949
LmnI GCTCC 4 cut(s) 78, 147, 563, 986
Lsp1109I GCAGC 4 cut(s) 494, 668, 808, 1055
LweI GCATC 5 cut(s) 277, 379, 608, 715, 833
MaeI CTAG 2 cut(s) 962, 1091
MaeII ACGT 3 cut(s) 250, 289, 935
MaeIII GTNAC 2 cut(s) 333, 613
MalI GATC 2 cut(s) 16, 951
MboI GATC 2 cut(s) 14, 949
MboII GAAGA 6 cut(s) 65, 431, 520, 800, 898, 1075
MfeI CAATTG 1 cut(s) 315
MhlI GDGCHC 1 cut(s) 144
MluCI AATT 6 cut(s) 28, 67, 315, 408, 447, 719
MlyI GAGTC 1 cut(s) 799
MmeI TCCRAC 4 cut(s) 265, 286, 398, 964
MroI TCCGGA 1 cut(s) 127
MroNI GCCGGC 1 cut(s) 55
MroXI GAANNNNTTC 1 cut(s) 412
MseI TTAA 6 cut(s) 65, 213, 339, 383, 774, 782
MslI CAYNNNNRTG 1 cut(s) 604
MspA1I CMGCKG 1 cut(s) 659
MspCI CTTAAG 1 cut(s) 781
MspI CCGG 8 cut(s) 33, 52, 56, 90, 128, 145, 158, 1076
MspR9I CCNGG 2 cut(s) 146, 1077
MunI CAATTG 1 cut(s) 315
MvnI CGCG 1 cut(s) 24
MwoI GCNNNNNNNGC 5 cut(s) 491, 500, 662, 671, 1052
NaeI GCCGGC 1 cut(s) 57
NciI CCSGG 2 cut(s) 146, 1077
NdeII GATC 2 cut(s) 14, 949
NgoMIV GCCGGC 1 cut(s) 55
NlaIII CATG 4 cut(s) 458, 614, 917, 1052
NlaIV GGNNCC 2 cut(s) 162, 664
NmeAIII GCCGAG 2 cut(s) 691, 991
NmuCI GTSAC 1 cut(s) 333
NspI RCATGY 1 cut(s) 458
PagI TCATGA 1 cut(s) 1048
PciI ACATGT 1 cut(s) 454
PdiI GCCGGC 1 cut(s) 57
PdmI GAANNNNTTC 1 cut(s) 412
PfeI GAWTC 4 cut(s) 166, 172, 970, 1072
PfoI TCCNGGA 1 cut(s) 144
PinAI ACCGGT 1 cut(s) 89
PkrI GCNGC 5 cut(s) 313, 484, 658, 823, 1045
PleI GAGTC 1 cut(s) 799
PpsI GAGTC 1 cut(s) 799
PscI ACATGT 1 cut(s) 454
Psp124BI GAGCTC 1 cut(s) 144
PspFI CCCAGC 1 cut(s) 659
PspN4I GGNNCC 2 cut(s) 162, 664
PspPI GGNCC 3 cut(s) 125, 663, 749
PvuII CAGCTG 1 cut(s) 659
RsaI GTAC 1 cut(s) 391
RsaNI GTAC 1 cut(s) 390
RseI CAYNNNNRTG 1 cut(s) 604
SacI GAGCTC 1 cut(s) 144
SaqAI TTAA 6 cut(s) 65, 213, 339, 383, 774, 782
SatI GCNGC 5 cut(s) 312, 483, 657, 822, 1044
Sau3AI GATC 2 cut(s) 14, 949
Sau96I GGNCC 3 cut(s) 125, 663, 749
SchI GAGTC 1 cut(s) 799
ScrFI CCNGG 2 cut(s) 146, 1077
SduI GDGCHC 1 cut(s) 144
SfaNI GCATC 5 cut(s) 277, 379, 608, 715, 833
SinI GGWCC 1 cut(s) 125
SmiMI CAYNNNNRTG 1 cut(s) 604
SmlI CTYRAG 1 cut(s) 781
SmoI CTYRAG 1 cut(s) 781
Sse9I AATT 6 cut(s) 28, 67, 315, 408, 447, 719
SsiI CCGC 6 cut(s) 24, 115, 266, 311, 593, 707
SspMI CTAG 2 cut(s) 962, 1091
SstI GAGCTC 1 cut(s) 144
StyD4I CCNGG 2 cut(s) 144, 1075
StyI CCWWGG 1 cut(s) 1056
TaaI ACNGT 1 cut(s) 845
TaiI ACGT 3 cut(s) 253, 292, 938
TasI AATT 6 cut(s) 28, 67, 315, 408, 447, 719
TauI GCSGC 1 cut(s) 314
TfiI GAWTC 4 cut(s) 166, 172, 970, 1072
Tru1I TTAA 6 cut(s) 65, 213, 339, 383, 774, 782
Tru9I TTAA 6 cut(s) 65, 213, 339, 383, 774, 782
TscAI CASTG 1 cut(s) 304
TseFI GTSAC 1 cut(s) 333
TseI GCWGC 4 cut(s) 482, 656, 821, 1043
Tsp45I GTSAC 1 cut(s) 333
TspDTI ATGAA 2 cut(s) 899, 1065
TspGWI ACGGA 3 cut(s) 199, 446, 773
TspRI CASTG 1 cut(s) 304
Vha464I CTTAAG 1 cut(s) 781
VpaK11BI GGWCC 1 cut(s) 125
XapI RAATTY 3 cut(s) 67, 408, 719
XbaI TCTAGA 1 cut(s) 1090
XceI RCATGY 1 cut(s) 458
XmnI GAANNNNTTC 1 cut(s) 412
XspI CTAG 2 cut(s) 962, 1091
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.