RchiOBHm_Chr5g0032001

Reticulon-4-interacting protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0012404
Physical Location & Seq
Forward (+)
25704471 .. 25706276
1806 bp
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UTR
Exon/CDS
Intron
PRQ31120

Sequence Viewer

Length: 465 bp
ATGTGCTATGGTGGTAATACCCTGAGAATAATTAATAGTGGGAAAATTCTTAAGGATATGGCATTGACAATAAAGGGGAAGTTTGATGCTGTCTTGGACACCATTGGTGGGTCAGAGACGGAAAGAATAAGTGTAAGTTTGTTAAAGAAAGGCGGACACTATATGACACTTCAGGGTGAGGCAGCATCTTTGACTGATAGATATGGGACAGCAATTGGGCTTCCAGTTGCTACTGCTGTTTTACTGAAGAAACAGATCCAGTACCGGTCTTCTCATGGAATAGAGTACTGGTGGACCTTCATGAGAGCAGATTCAGAAGGGTTACATAAGATCCGGAGGCTATCTGAAGCTGGAAAGTTAAACATACCTGTGGAGAAAACATTTCCCATCACTCAAGTGAGAGAGGCTCATGAGGCAAAGGAGAAGAAGCAGATTTATGGTAAAGTAGTGCTCGAACTTGACTGA

Protein Analysis

154

Amino Acids

17.15

Weight (kDa)

9.41

Isoelectric Point (pI)

34.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_zinc_N_2 PF13602 24 - 151 9.2e-19 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012404)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15090
fragaria_vesca FvH4_3g19130
malus_domestica MD03G1236200.v1.1 MD11G1257100.v1.1
prunus_persica Prupe.4G173000_v2.0.a1
pyrus_communis pycom03g18240 pycom11g22720
rosa_chinensis RchiOBHm_Chr5g0031931 RchiOBHm_Chr5g0032001 RchiOBHm_Chr5g0032041
rosa_laevigata RLG00000033359
rosa_multiflora Rmu_sc0003031.1_g000010 Rmu_sc0005375.1_g000023
rosa_roxburghii Rroxscaffold_1G00048330
rosa_rugosa Rorug05G0129400
rosa_samantha Rh5CG246600 Rh5DG228300
rosa_wichuraiana Rw5G020340 Rw5G020360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 333
AciI CCGC 1 cut(s) 153
AclWI GGATC 2 cut(s) 250, 325
AcsI RAATTY 1 cut(s) 45
AcuI CTGAAG 3 cut(s) 155, 266, 366
AfaI GTAC 2 cut(s) 263, 287
AfiI CCNNNNNNNGG 1 cut(s) 108
AflII CTTAAG 1 cut(s) 50
AgeI ACCGGT 1 cut(s) 264
AleI CACNNNNGTG 1 cut(s) 395
AluBI AGCT 1 cut(s) 350
AluI AGCT 1 cut(s) 350
Alw21I GWGCWC 1 cut(s) 453
Alw26I GTCTC 1 cut(s) 110
AlwI GGATC 2 cut(s) 250, 325
Aor13HI TCCGGA 1 cut(s) 333
ApeKI GCWGC 1 cut(s) 182
ApoI RAATTY 1 cut(s) 45
AseI ATTAAT 1 cut(s) 33
AsiGI ACCGGT 1 cut(s) 264
AspS9I GGNCC 1 cut(s) 294
AsuHPI GGTGA 1 cut(s) 188
AvaII GGWCC 1 cut(s) 294
BbsI GAAGAC 1 cut(s) 261
Bbv12I GWGCWC 1 cut(s) 453
BbvI GCAGC 1 cut(s) 194
BccI CCATC 1 cut(s) 395
BcoDI GTCTC 1 cut(s) 110
BfrI CTTAAG 1 cut(s) 50
BisI GCNGC 1 cut(s) 183
BlsI GCNGC 1 cut(s) 184
BmcAI AGTACT 1 cut(s) 287
Bme18I GGWCC 1 cut(s) 294
BmgT120I GGNCC 1 cut(s) 294
BmsI GCATC 2 cut(s) 76, 194
BpiI GAAGAC 1 cut(s) 261
BplI GAGNNNNNCTC 2 cut(s) 391, 423
BpuEI CTTGAG 1 cut(s) 378
BsaWI WCCGGW 2 cut(s) 264, 333
Bsc4I CCNNNNNNNGG 1 cut(s) 108
Bse118I RCCGGY 1 cut(s) 264
Bse1I ACTGG 3 cut(s) 224, 259, 293
BseAI TCCGGA 1 cut(s) 333
BseLI CCNNNNNNNGG 1 cut(s) 108
BseMII CTCAG 1 cut(s) 14
BseNI ACTGG 3 cut(s) 224, 259, 293
BseXI GCAGC 1 cut(s) 194
BshTI ACCGGT 1 cut(s) 264
BsiHKAI GWGCWC 1 cut(s) 453
BsiSI CCGG 2 cut(s) 265, 334
BslFI GGGAC 1 cut(s) 220
BslI CCNNNNNNNGG 1 cut(s) 108
BsmAI GTCTC 1 cut(s) 110
BsmBI CGTCTC 1 cut(s) 110
BsmFI GGGAC 1 cut(s) 220
Bsp1286I GDGCHC 1 cut(s) 453
Bsp13I TCCGGA 1 cut(s) 333
Bsp143I GATC 2 cut(s) 255, 330
BspACI CCGC 1 cut(s) 153
BspCNI CTCAG 1 cut(s) 15
BspEI TCCGGA 1 cut(s) 333
BspHI TCATGA 2 cut(s) 300, 409
BspPI GGATC 2 cut(s) 250, 325
BspTI CTTAAG 1 cut(s) 50
BsrFI RCCGGY 1 cut(s) 264
BsrI ACTGG 3 cut(s) 224, 259, 293
BssAI RCCGGY 1 cut(s) 264
BssMI GATC 2 cut(s) 255, 330
BstAFI CTTAAG 1 cut(s) 50
BstDEI CTNAG 1 cut(s) 23
BstKTI GATC 2 cut(s) 258, 333
BstMAI GTCTC 1 cut(s) 110
BstMBI GATC 2 cut(s) 255, 330
BstMWI GCNNNNNNNGC 1 cut(s) 413
BstV1I GCAGC 1 cut(s) 194
BstV2I GAAGAC 1 cut(s) 261
BstX2I RGATCY 2 cut(s) 255, 330
BstYI RGATCY 2 cut(s) 255, 330
CciI TCATGA 2 cut(s) 300, 409
Cfr10I RCCGGY 1 cut(s) 264
Cfr13I GGNCC 1 cut(s) 294
Csp6I GTAC 2 cut(s) 262, 286
CspAI ACCGGT 1 cut(s) 264
CviAII CATG 3 cut(s) 275, 301, 410
CviJI RGCY 4 cut(s) 220, 340, 350, 407
CviKI_1 RGCY 4 cut(s) 220, 340, 350, 407
CviQI GTAC 2 cut(s) 262, 286
DdeI CTNAG 1 cut(s) 23
DpnI GATC 2 cut(s) 257, 332
DpnII GATC 2 cut(s) 255, 330
EciI GGCGGA 1 cut(s) 168
Eco47I GGWCC 1 cut(s) 294
Eco57I CTGAAG 3 cut(s) 155, 266, 366
Esp3I CGTCTC 1 cut(s) 110
FaeI CATG 3 cut(s) 278, 304, 413
FaqI GGGAC 1 cut(s) 220
FatI CATG 3 cut(s) 274, 300, 409
Fnu4HI GCNGC 1 cut(s) 183
Fsp4HI GCNGC 1 cut(s) 183
GluI GCNGC 1 cut(s) 183
HapII CCGG 2 cut(s) 265, 334
Hin1II CATG 3 cut(s) 278, 304, 413
HinfI GANTC 1 cut(s) 311
HpaII CCGG 2 cut(s) 265, 334
HphI GGTGA 1 cut(s) 188
Hpy166II GTNNAC 1 cut(s) 294
Hpy188I TCNGA 3 cut(s) 115, 316, 346
Hpy188III TCNNGA 3 cut(s) 301, 334, 410
Hpy8I GTNNAC 1 cut(s) 294
HpyAV CCTTC 2 cut(s) 307, 311
HpyF10VI GCNNNNNNNGC 1 cut(s) 413
HpyF3I CTNAG 1 cut(s) 23
Hsp92II CATG 3 cut(s) 278, 304, 413
Kpn2I TCCGGA 1 cut(s) 333
Kzo9I GATC 2 cut(s) 255, 330
LpnPI CCDG 9 cut(s) 35, 158, 237, 272, 274, 278, 336, 347, 381
Lsp1109I GCAGC 1 cut(s) 194
LweI GCATC 2 cut(s) 76, 194
MaeIII GTNAC 1 cut(s) 321
MalI GATC 2 cut(s) 257, 332
MboI GATC 2 cut(s) 255, 330
MboII GAAGA 3 cut(s) 259, 261, 436
MfeI CAATTG 1 cut(s) 213
MflI RGATCY 2 cut(s) 255, 330
MhlI GDGCHC 1 cut(s) 453
MluCI AATT 3 cut(s) 30, 45, 213
MnlI CCTC 4 cut(s) 172, 330, 397, 406
MroI TCCGGA 1 cut(s) 333
MseI TTAA 4 cut(s) 33, 51, 143, 359
MslI CAYNNNNRTG 2 cut(s) 368, 395
MspCI CTTAAG 1 cut(s) 50
MspI CCGG 2 cut(s) 265, 334
MunI CAATTG 1 cut(s) 213
MwoI GCNNNNNNNGC 1 cut(s) 413
NdeII GATC 2 cut(s) 255, 330
NlaIII CATG 3 cut(s) 278, 304, 413
OliI CACNNNNGTG 1 cut(s) 395
PagI TCATGA 2 cut(s) 300, 409
PfeI GAWTC 1 cut(s) 311
PinAI ACCGGT 1 cut(s) 264
PkrI GCNGC 1 cut(s) 184
PshBI ATTAAT 1 cut(s) 33
PspPI GGNCC 1 cut(s) 294
PsuI RGATCY 2 cut(s) 255, 330
RsaI GTAC 2 cut(s) 263, 287
RsaNI GTAC 2 cut(s) 262, 286
RseI CAYNNNNRTG 2 cut(s) 368, 395
SaqAI TTAA 4 cut(s) 33, 51, 143, 359
SatI GCNGC 1 cut(s) 183
Sau3AI GATC 2 cut(s) 255, 330
Sau96I GGNCC 1 cut(s) 294
ScaI AGTACT 1 cut(s) 287
SduI GDGCHC 1 cut(s) 453
SetI ASST 3 cut(s) 299, 352, 370
SfaNI GCATC 2 cut(s) 76, 194
SinI GGWCC 1 cut(s) 294
SmiMI CAYNNNNRTG 2 cut(s) 368, 395
SmlI CTYRAG 2 cut(s) 50, 393
SmoI CTYRAG 2 cut(s) 50, 393
Sse9I AATT 3 cut(s) 30, 45, 213
SsiI CCGC 1 cut(s) 153
TaqI TCGA 1 cut(s) 453
TasI AATT 3 cut(s) 30, 45, 213
TatI WGTACW 1 cut(s) 285
TfiI GAWTC 1 cut(s) 311
Tru1I TTAA 4 cut(s) 33, 51, 143, 359
Tru9I TTAA 4 cut(s) 33, 51, 143, 359
TseI GCWGC 1 cut(s) 182
TspDTI ATGAA 1 cut(s) 289
TspGWI ACGGA 1 cut(s) 134
Vha464I CTTAAG 1 cut(s) 50
VpaK11BI GGWCC 1 cut(s) 294
VspI ATTAAT 1 cut(s) 33
XapI RAATTY 1 cut(s) 45
ZrmI AGTACT 1 cut(s) 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.