AT3G16370

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005825
Physical Location & Seq
Forward (+)
5556710 .. 5558982
2273 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G16370.1

Sequence Viewer

Length: 1062 bp
ATGGATCGTTGCACGTCGTCGTTTTTGCTTCTCACATTGGTCTCTACCTTGTCCATTCTCCAAATCTCCTTTGCTCAACTTGTTCCAGCAATCATGACATTCGGTGACTCTGTAGTTGATGTCGGCAACAACAACTATCTTCCGACCCTTTTCAGAGCTGATTATCCTCCTTATGGCCGTGATTTCGCTAACCACAAAGCCACCGGTCGTTTCTGCAACGGCAAATTAGCCACTGATATAACTGCTGAGACTTTAGGCTTCACTAAGTACCCACCAGCTTATCTAAGTCCTGAAGCTTCTGGTAAGAATCTTCTCATTGGTGCTAATTTCGCTTCTGCAGCTTCAGGTTATGATGACAAAGCTGCTCTTCTCAATCACGCGATCCCGTTGTATCAGCAAGTAGAGTATTTCAAGGAATACAAGAGCAAGCTAATAAAAATTGCAGGAAGCAAAAAAGCTGATTCGATCATAAAGGGAGCAATCTGTCTCTTAAGTGCAGGAAGCAGTGATTTTGTTCAAAACTACTATGTGAATCCTCTGCTTTACAAAGTTTACACTGTTGATGCATACGGATCTTTCCTTATCGATAACTTCTCTACATTTATCAAGCAAGTGTATGCGGTTGGAGCAAGGAAGATCGGTGTGACATCTCTGCCTCCAACAGGATGTCTTCCCGCTGCAAGAACCCTTTTCGGTTTCCATGAAAAAGGCTGTGTTTCAAGACTCAACACAGATGCTCAAAACTTTAACAAGAAGCTTAACGCTGCTGCTTCAAAGCTTCAGAAGCAATATTCCGATCTTAAGATTGTTGTCTTCGACATCTACTCTCCACTTTATGATCTTGTTCAGAACCCTTCCAAATCCGGATTCACGGAAGCAACCAAAGGATGTTGTGGAACAGGAACAGTCGAGACAACTTCACTCTTGTGCAACCCGAAATCGTTTGGGACATGCTCCAATGCTACTCAGTATGTGTTCTGGGACAGTGTGCATCCTTCTGAAGCTGCCAATGAGATTCTTGCCACTGCCCTAATTGGACAGGGCTTTTCTCTCCTCGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0048046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

353

Amino Acids

38.2

Weight (kDa)

8.58

Isoelectric Point (pI)

21.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 31 - 343 9.3e-35 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 380
AccIII TCCGGA 1 cut(s) 863
AciI CCGC 2 cut(s) 620, 675
AclWI GGATC 3 cut(s) 12, 376, 580
AcoI YGGCCR 1 cut(s) 175
AcuI CTGAAG 4 cut(s) 312, 327, 764, 1020
AfaI GTAC 1 cut(s) 269
AfiI CCNNNNNNNGG 2 cut(s) 173, 662
AflII CTTAAG 2 cut(s) 490, 800
AgeI ACCGGT 1 cut(s) 203
AgsI TTSAA 4 cut(s) 412, 518, 720, 774
AjiI CACGTC 1 cut(s) 15
AjuI GAANNNNNNNTTGG 2 cut(s) 851, 883
AleI CACNNNNGTG 1 cut(s) 925
Alw26I GTCTC 4 cut(s) 46, 242, 491, 905
AlwI GGATC 3 cut(s) 12, 376, 580
Aor13HI TCCGGA 1 cut(s) 863
AoxI GGCC 1 cut(s) 175
ApeKI GCWGC 6 cut(s) 338, 362, 677, 764, 767, 1004
AsiGI ACCGGT 1 cut(s) 203
AsuHPI GGTGA 1 cut(s) 116
BbsI GAAGAC 2 cut(s) 662, 805
BbvI GCAGC 6 cut(s) 349, 350, 664, 751, 754, 991
BceAI ACGGC 2 cut(s) 162, 235
BcoDI GTCTC 4 cut(s) 46, 242, 491, 905
BfmI CTRYAG 2 cut(s) 111, 336
BfrI CTTAAG 2 cut(s) 490, 800
BisI GCNGC 6 cut(s) 339, 363, 678, 765, 768, 1005
BlsI GCNGC 6 cut(s) 340, 364, 679, 766, 769, 1006
BmgBI CACGTC 1 cut(s) 15
BmsI GCATC 3 cut(s) 553, 724, 1000
BpiI GAAGAC 2 cut(s) 662, 805
Bsa29I ATCGAT 1 cut(s) 585
BsaI GGTCTC 1 cut(s) 46
BsaJI CCNNGG 1 cut(s) 1054
BsaWI WCCGGW 2 cut(s) 203, 863
Bsc4I CCNNNNNNNGG 2 cut(s) 173, 662
Bse118I RCCGGY 1 cut(s) 203
BseAI TCCGGA 1 cut(s) 863
BseCI ATCGAT 1 cut(s) 585
BseDI CCNNGG 1 cut(s) 1054
BseGI GGATG 3 cut(s) 671, 893, 991
BseLI CCNNNNNNNGG 2 cut(s) 173, 662
BseMII CTCAG 2 cut(s) 237, 980
BseRI GAGGAG 1 cut(s) 1043
BseXI GCAGC 6 cut(s) 349, 350, 664, 751, 754, 991
BsgI GTGCAG 1 cut(s) 516
Bsh1236I CGCG 1 cut(s) 380
Bsh1285I CGRYCG 1 cut(s) 208
BshFI GGCC 1 cut(s) 177
BshTI ACCGGT 1 cut(s) 203
BshVI ATCGAT 1 cut(s) 585
BsiEI CGRYCG 1 cut(s) 208
BsiSI CCGG 2 cut(s) 204, 864
BslFI GGGAC 2 cut(s) 961, 995
BslI CCNNNNNNNGG 2 cut(s) 173, 662
BsmAI GTCTC 4 cut(s) 46, 242, 491, 905
BsmFI GGGAC 2 cut(s) 961, 995
BsnI GGCC 1 cut(s) 177
Bso31I GGTCTC 1 cut(s) 46
Bsp13I TCCGGA 1 cut(s) 863
Bsp143I GATC 7 cut(s) 4, 381, 465, 572, 636, 796, 838
BspACI CCGC 2 cut(s) 620, 675
BspANI GGCC 1 cut(s) 177
BspCNI CTCAG 2 cut(s) 238, 979
BspDI ATCGAT 1 cut(s) 585
BspEI TCCGGA 1 cut(s) 863
BspFNI CGCG 1 cut(s) 380
BspHI TCATGA 1 cut(s) 93
BspMAI CTGCAG 1 cut(s) 340
BspPI GGATC 3 cut(s) 12, 376, 580
BspQI GCTCTTC 1 cut(s) 372
BspTI CTTAAG 2 cut(s) 490, 800
BspTNI GGTCTC 1 cut(s) 46
BsrFI RCCGGY 1 cut(s) 203
BssAI RCCGGY 1 cut(s) 203
BssECI CCNNGG 1 cut(s) 1054
BssMI GATC 7 cut(s) 4, 381, 465, 572, 636, 796, 838
Bst4CI ACNGT 3 cut(s) 559, 907, 986
Bst6I CTCTTC 1 cut(s) 372
BstAFI CTTAAG 2 cut(s) 490, 800
BstC8I GCNNGC 1 cut(s) 428
BstDEI CTNAG 4 cut(s) 246, 264, 284, 966
BstENI CCTNNNNNAGG 1 cut(s) 660
BstF5I GGATG 3 cut(s) 671, 893, 991
BstFNI CGCG 1 cut(s) 380
BstKTI GATC 7 cut(s) 7, 384, 468, 575, 639, 799, 841
BstMAI GTCTC 4 cut(s) 46, 242, 491, 905
BstMBI GATC 7 cut(s) 4, 381, 465, 572, 636, 796, 838
BstMCI CGRYCG 1 cut(s) 208
BstMWI GCNNNNNNNGC 4 cut(s) 329, 338, 626, 784
BstNSI RCATGY 1 cut(s) 954
BstSFI CTRYAG 2 cut(s) 111, 336
BstUI CGCG 1 cut(s) 380
BstV1I GCAGC 6 cut(s) 349, 350, 664, 751, 754, 991
BstV2I GAAGAC 2 cut(s) 662, 805
BstX2I RGATCY 1 cut(s) 572
BstYI RGATCY 1 cut(s) 572
Bsu15I ATCGAT 1 cut(s) 585
BsuRI GGCC 1 cut(s) 177
BsuTUI ATCGAT 1 cut(s) 585
BtrI CACGTC 1 cut(s) 15
BtsCI GGATG 3 cut(s) 671, 893, 991
BtsI GCAGTG 2 cut(s) 511, 1023
BtsIMutI CAGTG 5 cut(s) 231, 511, 555, 991, 1023
Cac8I GCNNGC 1 cut(s) 428
CciI TCATGA 1 cut(s) 93
Cfr10I RCCGGY 1 cut(s) 203
ClaI ATCGAT 1 cut(s) 585
Csp6I GTAC 1 cut(s) 268
CspAI ACCGGT 1 cut(s) 203
CviAII CATG 3 cut(s) 94, 701, 951
CviQI GTAC 1 cut(s) 268
DdeI CTNAG 4 cut(s) 246, 264, 284, 966
DpnI GATC 7 cut(s) 6, 383, 467, 574, 638, 798, 840
DpnII GATC 7 cut(s) 4, 381, 465, 572, 636, 796, 838
EaeI YGGCCR 1 cut(s) 175
Eam1104I CTCTTC 1 cut(s) 372
EarI CTCTTC 1 cut(s) 372
Eco31I GGTCTC 1 cut(s) 46
Eco57I CTGAAG 4 cut(s) 312, 327, 764, 1020
EcoNI CCTNNNNNAGG 1 cut(s) 660
EcoT22I ATGCAT 1 cut(s) 568
FaeI CATG 3 cut(s) 97, 704, 954
FalI AAGNNNNNCTT 2 cut(s) 351, 383
FaqI GGGAC 2 cut(s) 961, 995
FatI CATG 3 cut(s) 93, 700, 950
FauI CCCGC 1 cut(s) 682
Fnu4HI GCNGC 6 cut(s) 339, 363, 678, 765, 768, 1005
FokI GGATG 3 cut(s) 678, 900, 978
Fsp4HI GCNGC 6 cut(s) 339, 363, 678, 765, 768, 1005
GluI GCNGC 6 cut(s) 339, 363, 678, 765, 768, 1005
HaeIII GGCC 1 cut(s) 177
HapII CCGG 2 cut(s) 204, 864
Hin1II CATG 3 cut(s) 97, 704, 954
HindIII AAGCTT 3 cut(s) 294, 755, 776
HinfI GANTC 7 cut(s) 107, 307, 461, 532, 723, 867, 1015
HpaII CCGG 2 cut(s) 204, 864
HphI GGTGA 1 cut(s) 116
Hpy166II GTNNAC 1 cut(s) 553
Hpy188I TCNGA 6 cut(s) 144, 155, 783, 796, 849, 1000
Hpy188III TCNNGA 5 cut(s) 94, 290, 720, 864, 910
Hpy8I GTNNAC 1 cut(s) 553
Hpy99I CGWCG 2 cut(s) 19, 22
HpyAV CCTTC 2 cut(s) 864, 1005
HpyCH4III ACNGT 3 cut(s) 559, 907, 986
HpyCH4IV ACGT 1 cut(s) 14
HpyCH4V TGCA 9 cut(s) 12, 216, 338, 443, 497, 566, 680, 930, 991
HpyF10VI GCNNNNNNNGC 4 cut(s) 329, 338, 626, 784
HpyF3I CTNAG 4 cut(s) 246, 264, 284, 966
HpySE526I ACGT 1 cut(s) 14
Hsp92II CATG 3 cut(s) 97, 704, 954
Kpn2I TCCGGA 1 cut(s) 863
Kzo9I GATC 7 cut(s) 4, 381, 465, 572, 636, 796, 838
LguI GCTCTTC 1 cut(s) 372
LmnI GCTCC 3 cut(s) 476, 626, 959
Lsp1109I GCAGC 6 cut(s) 349, 350, 664, 751, 754, 991
LweI GCATC 3 cut(s) 553, 724, 1000
MaeII ACGT 1 cut(s) 14
MaeIII GTNAC 2 cut(s) 104, 643
MalI GATC 7 cut(s) 6, 383, 467, 574, 638, 798, 840
MboI GATC 7 cut(s) 4, 381, 465, 572, 636, 796, 838
MboII GAAGA 6 cut(s) 131, 302, 359, 646, 662, 805
MflI RGATCY 1 cut(s) 572
MluCI AATT 4 cut(s) 224, 325, 438, 1032
MlyI GAGTC 2 cut(s) 101, 717
MmeI TCCRAC 3 cut(s) 167, 604, 683
MnlI CCTC 3 cut(s) 177, 546, 666
Mph1103I ATGCAT 1 cut(s) 568
MroI TCCGGA 1 cut(s) 863
MseI TTAA 4 cut(s) 491, 747, 759, 801
MslI CAYNNNNRTG 1 cut(s) 925
MspA1I CMGCKG 1 cut(s) 677
MspCI CTTAAG 2 cut(s) 490, 800
MspI CCGG 2 cut(s) 204, 864
MvnI CGCG 1 cut(s) 380
MwoI GCNNNNNNNGC 4 cut(s) 329, 338, 626, 784
NdeII GATC 7 cut(s) 4, 381, 465, 572, 636, 796, 838
NlaIII CATG 3 cut(s) 97, 704, 954
NmuCI GTSAC 2 cut(s) 104, 643
NsiI ATGCAT 1 cut(s) 568
NspI RCATGY 1 cut(s) 954
OliI CACNNNNGTG 1 cut(s) 925
PagI TCATGA 1 cut(s) 93
PciSI GCTCTTC 1 cut(s) 372
PfeI GAWTC 5 cut(s) 307, 461, 532, 867, 1015
PinAI ACCGGT 1 cut(s) 203
PkrI GCNGC 6 cut(s) 340, 364, 679, 766, 769, 1006
PleI GAGTC 2 cut(s) 101, 717
PpsI GAGTC 2 cut(s) 101, 717
PstI CTGCAG 1 cut(s) 340
PsuI RGATCY 1 cut(s) 572
RsaI GTAC 1 cut(s) 269
RsaNI GTAC 1 cut(s) 268
RseI CAYNNNNRTG 1 cut(s) 925
SapI GCTCTTC 1 cut(s) 372
SaqAI TTAA 4 cut(s) 491, 747, 759, 801
SatI GCNGC 6 cut(s) 339, 363, 678, 765, 768, 1005
Sau3AI GATC 7 cut(s) 4, 381, 465, 572, 636, 796, 838
SchI GAGTC 2 cut(s) 101, 717
SfaNI GCATC 3 cut(s) 553, 724, 1000
SfcI CTRYAG 2 cut(s) 111, 336
SmiMI CAYNNNNRTG 1 cut(s) 925
SmlI CTYRAG 2 cut(s) 490, 800
SmoI CTYRAG 2 cut(s) 490, 800
Sse9I AATT 4 cut(s) 224, 325, 438, 1032
SsiI CCGC 2 cut(s) 620, 675
SspI AATATT 1 cut(s) 791
TaaI ACNGT 3 cut(s) 559, 907, 986
TaiI ACGT 1 cut(s) 17
TaqI TCGA 4 cut(s) 464, 585, 816, 909
TasI AATT 4 cut(s) 224, 325, 438, 1032
TfiI GAWTC 5 cut(s) 307, 461, 532, 867, 1015
Tru1I TTAA 4 cut(s) 491, 747, 759, 801
Tru9I TTAA 4 cut(s) 491, 747, 759, 801
TscAI CASTG 5 cut(s) 238, 511, 562, 991, 1030
TseFI GTSAC 2 cut(s) 104, 643
TseI GCWGC 6 cut(s) 338, 362, 677, 764, 767, 1004
Tsp45I GTSAC 2 cut(s) 104, 643
TspDTI ATGAA 1 cut(s) 717
TspGWI ACGGA 2 cut(s) 585, 887
TspRI CASTG 5 cut(s) 238, 511, 562, 991, 1030
Vha464I CTTAAG 2 cut(s) 490, 800
XagI CCTNNNNNAGG 1 cut(s) 660
XceI RCATGY 1 cut(s) 954
Zsp2I ATGCAT 1 cut(s) 568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.