MD17G1180300.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0005825
Physical Location & Seq
Forward (+)
20956382 .. 20958942
2561 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1180300.v1.1.491

Sequence Viewer

Length: 1074 bp
ATGGAAAGCCGTTTCTTGAGCGCAGCTTTTGGTTTGGTTTTGGTTTTTGCGTTTGTGATTGGAGGGAATTATGCACAAGAGTCAACTACACTTGTTCCGGCAATCATGACTTTTGGAGACTCAGCAGTTGATGTGGGCAACAATGACTACCTCCCTACCATTTTCAAGGCCGATTACCCTCCTTATGGAAGGGACTTTGTGAACCATCAGCCTACTGGGAGGTTTTGCAATGGCAAACTGGCCACTGACATCACTGCTGACACTCTAGGGTTCAGTACTTATCCGCCGGCGTACCTTAGCCCACAAGCATCAGGGAAGAACCTTCTGATCGGAGCCAACTTCGCTTCAGCTGCATCCGGTTACGATGATAAGGCAGCGACCTTAAGTCATGCAATCCCATTGTCCCAGCAGCTGCAATATTACAAGGAGTACCAGACGAAGCTTGCCAAGGTAGCAGGCAGCATGAAAGCGGCATCGATCATCAAGGATGCTCTCTATTTACTGAGTGCCGGAAACAGTGACTTTCTTCAGAATTACTACGTCAACCCTTTTGTTAACAAAGTCTACACTCCCGATCAGTATGGTTCCATCCTTGTTGGTGTCTTCCAAGGCTTCATTACGGATTTGTATCACTTGGGAGCCAGGAAGATCGGGGTGACCTCACTCCCTCCATTGGGTTGCCTCCCGGCTGCAATCACCTTATTTGGAAATCATGAGCAGGGATGCGTCGCTAGAATCAACTCCGACGCCCAAGGGTTTAACAAGAAGATAACCTCTGCCGCAGACAATCTCCAAAAGCAACTTTCAGGCCTTAAAGTCGTCATCTTCGACATTTACAAGCCTCTCTACGATGTCATTAAAGCCCCAGCAAATTACGGATTCGCCGAAGCAAGAAGAGGTTGCTGTGGAACGGGAATCGTAGAGACAACGTCACTGCTGTGCAATCCAAACTCAGTCGGAACTTGTTCCAACGCGACTCAGTACGTGTTTTGGGATAGCGTCCATCCATCTCAAGCCGCTAACCAAGTGCTTGCCGATGCATTGATCATCCAAGGCATCTCCCTCATTGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0048046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

358

Amino Acids

38.25

Weight (kDa)

6.5

Isoelectric Point (pI)

22.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 35 - 348 2.4e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 564
AccII CGCG 1 cut(s) 974
AciI CCGC 4 cut(s) 284, 470, 780, 1017
AcoI YGGCCR 1 cut(s) 240
AcuI CTGAAG 2 cut(s) 330, 512
AcyI GRCGYC 1 cut(s) 747
AfaI GTAC 4 cut(s) 277, 293, 431, 983
AfiI CCNNNNNNNGG 3 cut(s) 185, 673, 674
AflII CTTAAG 1 cut(s) 382
AflIII ACRYGT 1 cut(s) 984
AgsI TTSAA 1 cut(s) 166
AhdI GACNNNNNGTC 1 cut(s) 384
AjnI CCWGG 1 cut(s) 641
AleI CACNNNNGTG 1 cut(s) 937
AluBI AGCT 4 cut(s) 26, 350, 412, 442
AluI AGCT 4 cut(s) 26, 350, 412, 442
Alw26I GTCTC 2 cut(s) 111, 917
AlwNI CAGNNNCTG 1 cut(s) 412
AoxI GGCC 3 cut(s) 168, 240, 808
ApeKI GCWGC 7 cut(s) 23, 350, 374, 409, 412, 459, 689
Asp700I GAANNNNTTC 1 cut(s) 964
AspLEI GCGC 1 cut(s) 23
AsuC2I CCSGG 1 cut(s) 686
AsuHPI GGTGA 2 cut(s) 667, 688
BalI TGGCCA 1 cut(s) 242
BbsI GAAGAC 1 cut(s) 595
BbvI GCAGC 7 cut(s) 35, 337, 386, 399, 421, 471, 676
BccI CCATC 4 cut(s) 213, 596, 1011, 1015
BciT130I CCWGG 1 cut(s) 643
BclI TGATCA 1 cut(s) 1044
BcnI CCSGG 1 cut(s) 686
BcoDI GTCTC 2 cut(s) 111, 917
BfaI CTAG 2 cut(s) 266, 732
BfrI CTTAAG 1 cut(s) 382
BmcAI AGTACT 1 cut(s) 277
Bme1390I CCNGG 2 cut(s) 643, 686
BmeRI GACNNNNNGTC 1 cut(s) 384
BmiI GGNNCC 3 cut(s) 334, 586, 640
BmrFI CCNGG 2 cut(s) 643, 686
BmrI ACTGGG 1 cut(s) 225
BmsI GCATC 7 cut(s) 317, 362, 478, 482, 713, 1027, 1065
BmuI ACTGGG 1 cut(s) 225
BpiI GAAGAC 1 cut(s) 595
Bpu10I CCTNAGC 1 cut(s) 296
BpuEI CTTGAG 2 cut(s) 37, 996
BpuMI CCSGG 1 cut(s) 686
Bsa29I ATCGAT 1 cut(s) 476
BsaAI YACGTR 1 cut(s) 985
BsaBI GATNNNNATC 1 cut(s) 627
BsaHI GRCGYC 1 cut(s) 747
BsaJI CCNNGG 4 cut(s) 447, 607, 751, 1051
BsaWI WCCGGW 1 cut(s) 356
Bsc4I CCNNNNNNNGG 3 cut(s) 185, 673, 674
Bse118I RCCGGY 1 cut(s) 286
Bse1I ACTGG 2 cut(s) 220, 243
Bse3DI GCAATG 1 cut(s) 235
Bse8I GATNNNNATC 1 cut(s) 627
BseBI CCWGG 1 cut(s) 643
BseCI ATCGAT 1 cut(s) 476
BseDI CCNNGG 4 cut(s) 447, 607, 751, 1051
BseGI GGATG 6 cut(s) 353, 493, 588, 728, 1003, 1047
BseJI GATNNNNATC 1 cut(s) 627
BseLI CCNNNNNNNGG 3 cut(s) 185, 673, 674
BseMI GCAATG 1 cut(s) 235
BseMII CTCAG 4 cut(s) 135, 494, 966, 992
BseNI ACTGG 2 cut(s) 220, 243
BseXI GCAGC 7 cut(s) 35, 337, 386, 399, 421, 471, 676
BseYI CCCAGC 2 cut(s) 405, 865
Bsh1236I CGCG 1 cut(s) 974
BshFI GGCC 3 cut(s) 170, 242, 810
BshVI ATCGAT 1 cut(s) 476
BsiSI CCGG 5 cut(s) 98, 287, 357, 510, 686
BslFI GGGAC 2 cut(s) 206, 388
BslI CCNNNNNNNGG 3 cut(s) 185, 673, 674
BsmAI GTCTC 2 cut(s) 111, 917
BsmFI GGGAC 2 cut(s) 206, 388
BsnI GGCC 3 cut(s) 170, 242, 810
Bsp143I GATC 5 cut(s) 327, 477, 574, 648, 1044
BspACI CCGC 4 cut(s) 284, 470, 780, 1017
BspANI GGCC 3 cut(s) 170, 242, 810
BspCNI CTCAG 4 cut(s) 134, 495, 965, 991
BspDI ATCGAT 1 cut(s) 476
BspFNI CGCG 1 cut(s) 974
BspHI TCATGA 2 cut(s) 105, 712
BspLI GGNNCC 3 cut(s) 334, 586, 640
BspTI CTTAAG 1 cut(s) 382
BsrDI GCAATG 1 cut(s) 235
BsrFI RCCGGY 1 cut(s) 286
BsrI ACTGG 2 cut(s) 220, 243
BssAI RCCGGY 1 cut(s) 286
BssECI CCNNGG 4 cut(s) 447, 607, 751, 1051
BssMI GATC 5 cut(s) 327, 477, 574, 648, 1044
BssNI GRCGYC 1 cut(s) 747
BssT1I CCWWGG 4 cut(s) 447, 607, 751, 1051
Bst2UI CCWGG 1 cut(s) 643
Bst4CI ACNGT 1 cut(s) 518
Bst6I CTCTTC 1 cut(s) 889
BstACI GRCGYC 1 cut(s) 747
BstAFI CTTAAG 1 cut(s) 382
BstBAI YACGTR 1 cut(s) 985
BstC8I GCNNGC 4 cut(s) 288, 444, 457, 1032
BstDEI CTNAG 5 cut(s) 121, 296, 503, 952, 978
BstEII GGTNACC 1 cut(s) 655
BstF5I GGATG 6 cut(s) 353, 493, 588, 728, 1003, 1047
BstFNI CGCG 1 cut(s) 974
BstHHI GCGC 1 cut(s) 23
BstKTI GATC 5 cut(s) 330, 480, 577, 651, 1047
BstMAI GTCTC 2 cut(s) 111, 917
BstMBI GATC 5 cut(s) 327, 477, 574, 648, 1044
BstMWI GCNNNNNNNGC 3 cut(s) 341, 350, 452
BstNI CCWGG 1 cut(s) 643
BstPI GGTNACC 1 cut(s) 655
BstSCI CCNGG 2 cut(s) 641, 684
BstUI CGCG 1 cut(s) 974
BstV1I GCAGC 7 cut(s) 35, 337, 386, 399, 421, 471, 676
BstV2I GAAGAC 1 cut(s) 595
Bsu15I ATCGAT 1 cut(s) 476
BsuRI GGCC 3 cut(s) 170, 242, 810
BsuTUI ATCGAT 1 cut(s) 476
BtsCI GGATG 6 cut(s) 353, 493, 588, 728, 1003, 1047
BtsI GCAGTG 2 cut(s) 252, 932
BtsIMutI CAGTG 4 cut(s) 243, 252, 523, 932
Cac8I GCNNGC 4 cut(s) 288, 444, 457, 1032
CaiI CAGNNNCTG 1 cut(s) 412
CciI TCATGA 2 cut(s) 105, 712
CfoI GCGC 1 cut(s) 23
Cfr10I RCCGGY 1 cut(s) 286
ClaI ATCGAT 1 cut(s) 476
CseI GACGC 3 cut(s) 715, 755, 988
Csp6I GTAC 4 cut(s) 276, 292, 430, 982
CviAII CATG 4 cut(s) 106, 389, 463, 713
CviQI GTAC 4 cut(s) 276, 292, 430, 982
DdeI CTNAG 5 cut(s) 121, 296, 503, 952, 978
DpnI GATC 5 cut(s) 329, 479, 576, 650, 1046
DpnII GATC 5 cut(s) 327, 477, 574, 648, 1044
DriI GACNNNNNGTC 1 cut(s) 384
EaeI YGGCCR 1 cut(s) 240
Eam1104I CTCTTC 1 cut(s) 889
Eam1105I GACNNNNNGTC 1 cut(s) 384
EarI CTCTTC 1 cut(s) 889
EciI GGCGGA 1 cut(s) 273
Eco130I CCWWGG 4 cut(s) 447, 607, 751, 1051
Eco147I AGGCCT 1 cut(s) 810
Eco57I CTGAAG 2 cut(s) 330, 512
Eco91I GGTNACC 1 cut(s) 655
EcoO65I GGTNACC 1 cut(s) 655
EcoRII CCWGG 1 cut(s) 641
EcoT14I CCWWGG 4 cut(s) 447, 607, 751, 1051
EcoT22I ATGCAT 1 cut(s) 1042
ErhI CCWWGG 4 cut(s) 447, 607, 751, 1051
FaeI CATG 4 cut(s) 109, 392, 466, 716
FaiI YATR 7 cut(s) 72, 107, 186, 390, 464, 582, 714
FaqI GGGAC 2 cut(s) 206, 388
FatI CATG 4 cut(s) 105, 388, 462, 712
FbaI TGATCA 1 cut(s) 1044
FblI GTMKAC 1 cut(s) 564
FokI GGATG 6 cut(s) 340, 500, 575, 735, 990, 1034
FspBI CTAG 2 cut(s) 266, 732
GlaI GCGC 1 cut(s) 22
GsaI CCCAGC 2 cut(s) 409, 869
HaeIII GGCC 3 cut(s) 170, 242, 810
HapII CCGG 5 cut(s) 98, 287, 357, 510, 686
HgaI GACGC 3 cut(s) 715, 755, 988
HhaI GCGC 1 cut(s) 23
Hin1I GRCGYC 1 cut(s) 747
Hin1II CATG 4 cut(s) 109, 392, 466, 716
Hin6I GCGC 1 cut(s) 21
HinP1I GCGC 1 cut(s) 21
HincII GTYRAC 3 cut(s) 84, 544, 556
HindII GTYRAC 3 cut(s) 84, 544, 556
HindIII AAGCTT 1 cut(s) 440
HinfI GANTC 6 cut(s) 80, 119, 735, 879, 915, 976
HpaI GTTAAC 1 cut(s) 556
HpaII CCGG 5 cut(s) 98, 287, 357, 510, 686
HphI GGTGA 2 cut(s) 667, 688
Hpy166II GTNNAC 5 cut(s) 84, 202, 544, 556, 565
Hpy188I TCNGA 5 cut(s) 327, 332, 531, 745, 959
Hpy188III TCNNGA 4 cut(s) 16, 106, 572, 713
Hpy8I GTNNAC 5 cut(s) 84, 202, 544, 556, 565
Hpy99I CGWCG 2 cut(s) 731, 749
HpyAV CCTTC 2 cut(s) 183, 332
HpyCH4III ACNGT 1 cut(s) 518
HpyCH4IV ACGT 3 cut(s) 540, 929, 984
HpyCH4V TGCA 8 cut(s) 74, 228, 353, 392, 415, 692, 942, 1040
HpyF10VI GCNNNNNNNGC 3 cut(s) 341, 350, 452
HpyF3I CTNAG 5 cut(s) 121, 296, 503, 952, 978
HpySE526I ACGT 3 cut(s) 540, 929, 984
Hsp92I GRCGYC 1 cut(s) 747
Hsp92II CATG 4 cut(s) 109, 392, 466, 716
HspAI GCGC 1 cut(s) 21
KroI GCCGGC 1 cut(s) 286
KroNI GCCGGC 1 cut(s) 288
Ksp22I TGATCA 1 cut(s) 1044
KspAI GTTAAC 1 cut(s) 556
Kzo9I GATC 5 cut(s) 327, 477, 574, 648, 1044
LmnI GCTCC 2 cut(s) 332, 638
Lsp1109I GCAGC 7 cut(s) 35, 337, 386, 399, 421, 471, 676
LweI GCATC 7 cut(s) 317, 362, 478, 482, 713, 1027, 1065
MaeI CTAG 2 cut(s) 266, 732
MaeII ACGT 3 cut(s) 540, 929, 984
MaeIII GTNAC 4 cut(s) 359, 518, 655, 930
MalI GATC 5 cut(s) 329, 479, 576, 650, 1046
MboI GATC 5 cut(s) 327, 477, 574, 648, 1044
MboII GAAGA 7 cut(s) 328, 518, 595, 658, 778, 817, 906
MlsI TGGCCA 1 cut(s) 242
MluCI AATT 3 cut(s) 67, 532, 871
MluNI TGGCCA 1 cut(s) 242
MlyI GAGTC 3 cut(s) 89, 113, 970
MmeI TCCRAC 3 cut(s) 768, 937, 993
Mox20I TGGCCA 1 cut(s) 242
Mph1103I ATGCAT 1 cut(s) 1042
MreI CGCCGGCG 1 cut(s) 286
MroNI GCCGGC 1 cut(s) 286
MroXI GAANNNNTTC 1 cut(s) 964
MscI TGGCCA 1 cut(s) 242
MseI TTAA 5 cut(s) 383, 555, 759, 813, 858
MslI CAYNNNNRTG 1 cut(s) 937
Msp20I TGGCCA 1 cut(s) 242
MspA1I CMGCKG 2 cut(s) 350, 412
MspCI CTTAAG 1 cut(s) 382
MspI CCGG 5 cut(s) 98, 287, 357, 510, 686
MspR9I CCNGG 2 cut(s) 643, 686
MvaI CCWGG 1 cut(s) 643
MvnI CGCG 1 cut(s) 974
MwoI GCNNNNNNNGC 3 cut(s) 341, 350, 452
NaeI GCCGGC 1 cut(s) 288
NciI CCSGG 1 cut(s) 686
NdeII GATC 5 cut(s) 327, 477, 574, 648, 1044
NgoMIV GCCGGC 1 cut(s) 286
NlaIII CATG 4 cut(s) 109, 392, 466, 716
NlaIV GGNNCC 3 cut(s) 334, 586, 640
NmuCI GTSAC 3 cut(s) 518, 655, 930
NsiI ATGCAT 1 cut(s) 1042
OliI CACNNNNGTG 1 cut(s) 937
PagI TCATGA 2 cut(s) 105, 712
PceI AGGCCT 1 cut(s) 810
PcsI WCGNNNNNNNCGW 2 cut(s) 825, 882
PdiI GCCGGC 1 cut(s) 288
PdmI GAANNNNTTC 1 cut(s) 964
PfeI GAWTC 3 cut(s) 735, 879, 915
PflFI GACNNNGTC 1 cut(s) 928
PleI GAGTC 3 cut(s) 88, 113, 970
PpsI GAGTC 3 cut(s) 88, 113, 970
Ppu21I YACGTR 1 cut(s) 985
Psp6I CCWGG 1 cut(s) 641
PspEI GGTNACC 1 cut(s) 655
PspFI CCCAGC 2 cut(s) 405, 865
PspGI CCWGG 1 cut(s) 641
PspN4I GGNNCC 3 cut(s) 334, 586, 640
PstNI CAGNNNCTG 1 cut(s) 412
PsyI GACNNNGTC 1 cut(s) 928
PvuII CAGCTG 2 cut(s) 350, 412
RsaI GTAC 4 cut(s) 277, 293, 431, 983
RsaNI GTAC 4 cut(s) 276, 292, 430, 982
RseI CAYNNNNRTG 1 cut(s) 937
SaqAI TTAA 5 cut(s) 383, 555, 759, 813, 858
Sau3AI GATC 5 cut(s) 327, 477, 574, 648, 1044
ScaI AGTACT 1 cut(s) 277
SchI GAGTC 3 cut(s) 89, 113, 970
ScrFI CCNGG 2 cut(s) 643, 686
SfaNI GCATC 7 cut(s) 317, 362, 478, 482, 713, 1027, 1065
SgrAI CRCCGGYG 1 cut(s) 286
SmiMI CAYNNNNRTG 1 cut(s) 937
SmlI CTYRAG 3 cut(s) 16, 382, 1011
SmoI CTYRAG 3 cut(s) 16, 382, 1011
Sse9I AATT 3 cut(s) 67, 532, 871
SseBI AGGCCT 1 cut(s) 810
SsiI CCGC 4 cut(s) 284, 470, 780, 1017
SspI AATATT 1 cut(s) 419
SspMI CTAG 2 cut(s) 266, 732
StuI AGGCCT 1 cut(s) 810
StyD4I CCNGG 2 cut(s) 641, 684
StyI CCWWGG 4 cut(s) 447, 607, 751, 1051
TaaI ACNGT 1 cut(s) 518
TaiI ACGT 3 cut(s) 543, 932, 987
TaqI TCGA 2 cut(s) 476, 828
TasI AATT 3 cut(s) 67, 532, 871
TatI WGTACW 1 cut(s) 275
TauI GCSGC 3 cut(s) 473, 782, 1019
TfiI GAWTC 3 cut(s) 735, 879, 915
Tru1I TTAA 5 cut(s) 383, 555, 759, 813, 858
Tru9I TTAA 5 cut(s) 383, 555, 759, 813, 858
TscAI CASTG 4 cut(s) 250, 259, 523, 939
TseFI GTSAC 3 cut(s) 518, 655, 930
TseI GCWGC 7 cut(s) 23, 350, 374, 409, 412, 459, 689
Tsp45I GTSAC 3 cut(s) 518, 655, 930
TspDTI ATGAA 2 cut(s) 479, 604
TspGWI ACGGA 2 cut(s) 635, 891
TspRI CASTG 4 cut(s) 250, 259, 523, 939
Tth111I GACNNNGTC 1 cut(s) 928
Vha464I CTTAAG 1 cut(s) 382
XcmI CCANNNNNNNNNTGG 1 cut(s) 212
XmiI GTMKAC 1 cut(s) 564
XmnI GAANNNNTTC 1 cut(s) 964
XspI CTAG 2 cut(s) 266, 732
ZrmI AGTACT 1 cut(s) 277
Zsp2I ATGCAT 1 cut(s) 1042
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.