pycom09g11690

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0005825
Physical Location & Seq
Forward (+)
9898028 .. 9900130
2103 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g11690.1

Sequence Viewer

Length: 1077 bp
ATGGAAAGCCATTTCTTGAGAGCAGCAGCTTTTGGTTTGGTTTTGGCTTTTGCGTTTGTGATTGGAGCAAATTATGCACAAGAGTCAACCACACTTGTTCCGGCAATCATGACGTTTGGAGACTCTGCAGTTGATGTGGGCAACAATGACTACCTCCCTACCATTTTCAAGGCTAATTACCCTCCTTATGGAAGGGACTTTGTGAGCCATCAGCCTACTGGGAGGTTTTGCAATGGCAAACTGGCAACGGACATCACTGCCGACACTCTGGGGTTCACTACTTATCCGCCGGCGTATCTTAGCCCACAAGCATCAGGGAAAAACCTTCTGATCGGAGCCAACTTCGCTTCAGCTGCATCCGGTTACGATGAAAAGGCAGCAACCTTAAACCATGCAATCCCATTGTCCCAGCAGCTGCAATATTACAAGGAATACCAGACAAAGCTTGCCAAGGTAGCAGGCAGCAAGAAAGCGGCATCGATCATCAAGGATGCTCTCTATTTACTGAGTGCCGGAAACAGTGACTTTCTTCAGAATTACTATGTCAACCCTTTTGTTAACAAAGTCTACACTCCTGACCAGTATGGCTCCATCCTTGTTGGTGTCTTCCAAGGCTTCGTTAAGGATTTGTATCACTTGGGAGCCAGGAAGATCGGTGTGACCTCACTCCCTCCATTGGGTTGCCTTCCGGCTGCAATCACCTTATTTGGAAACCATGAGCAAGGATGCGTCGCTAGAATCAACTCTGATGCCCAAGGGTTTAACAAGAAGATAAACTCCGCAGCAGGAAATCTCCAAAAACAACTTTCCGGTCTTAAAATCGTCATCTTCGACATTTACAAGCCGCTCTATGATGCCATTAAAGCCCCAGCAAATTACGGATTCACCGAAGCGAGGAGAGGTTGCTGTGGAACGGGAATTGTAGAGACAACATCACTGCTGTGCAATCCCAAGTCGATCGGAACTTGTTCTAATGCGACTCAATACGTGTTTTGGGACAGCGTCCATCCGTCTGAAGCCGCTAACCAAGTTCTAGCCGATGCATTGATCCTCCAAGGCATCTCCCTCATTGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0048046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

359

Amino Acids

38.4

Weight (kDa)

8.33

Isoelectric Point (pI)

24.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 36 - 349 6.4e-33 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 847
AccI GTMKAC 1 cut(s) 567
AciI CCGC 5 cut(s) 287, 473, 780, 845, 1020
AclWI GGATC 1 cut(s) 1042
AcuI CTGAAG 3 cut(s) 333, 515, 1035
AfiI CCNNNNNNNGG 4 cut(s) 188, 676, 677, 894
AflIII ACRYGT 1 cut(s) 987
AgsI TTSAA 1 cut(s) 169
AjnI CCWGG 1 cut(s) 644
AleI CACNNNNGTG 1 cut(s) 940
AluBI AGCT 4 cut(s) 29, 353, 415, 445
AluI AGCT 4 cut(s) 29, 353, 415, 445
Alw26I GTCTC 2 cut(s) 114, 920
AlwI GGATC 1 cut(s) 1042
AlwNI CAGNNNCTG 1 cut(s) 415
ApeKI GCWGC 9 cut(s) 23, 26, 353, 377, 412, 415, 462, 692, 782
Asp700I GAANNNNTTC 1 cut(s) 967
AsuHPI GGTGA 2 cut(s) 691, 877
BbsI GAAGAC 1 cut(s) 598
BbvI GCAGC 9 cut(s) 35, 38, 340, 389, 402, 424, 474, 679, 794
BccI CCATC 3 cut(s) 216, 599, 1014
BciT130I CCWGG 1 cut(s) 646
BcoDI GTCTC 2 cut(s) 114, 920
BfaI CTAG 2 cut(s) 735, 1034
BfmI CTRYAG 1 cut(s) 126
Bme1390I CCNGG 1 cut(s) 646
BmiI GGNNCC 3 cut(s) 337, 589, 643
BmrFI CCNGG 1 cut(s) 646
BmrI ACTGGG 1 cut(s) 228
BmsI GCATC 9 cut(s) 320, 365, 481, 485, 716, 739, 844, 1030, 1068
BmuI ACTGGG 1 cut(s) 228
BpiI GAAGAC 1 cut(s) 598
BpuEI CTTGAG 1 cut(s) 37
Bsa29I ATCGAT 1 cut(s) 479
BsaAI YACGTR 1 cut(s) 988
BsaBI GATNNNNATC 1 cut(s) 630
BsaJI CCNNGG 4 cut(s) 450, 610, 754, 1054
BsaWI WCCGGW 2 cut(s) 359, 809
Bsc4I CCNNNNNNNGG 4 cut(s) 188, 676, 677, 894
Bse118I RCCGGY 1 cut(s) 289
Bse1I ACTGG 3 cut(s) 223, 246, 580
Bse3DI GCAATG 1 cut(s) 238
Bse8I GATNNNNATC 1 cut(s) 630
BseBI CCWGG 1 cut(s) 646
BseCI ATCGAT 1 cut(s) 479
BseDI CCNNGG 4 cut(s) 450, 610, 754, 1054
BseGI GGATG 5 cut(s) 356, 496, 591, 731, 1006
BseJI GATNNNNATC 1 cut(s) 630
BseLI CCNNNNNNNGG 4 cut(s) 188, 676, 677, 894
BseMI GCAATG 1 cut(s) 238
BseMII CTCAG 1 cut(s) 497
BseNI ACTGG 3 cut(s) 223, 246, 580
BseRI GAGGAG 1 cut(s) 910
BseXI GCAGC 9 cut(s) 35, 38, 340, 389, 402, 424, 474, 679, 794
BseYI CCCAGC 2 cut(s) 408, 868
Bsh1285I CGRYCG 1 cut(s) 960
BshVI ATCGAT 1 cut(s) 479
BsiEI CGRYCG 1 cut(s) 960
BsiSI CCGG 6 cut(s) 101, 290, 360, 513, 689, 810
BslFI GGGAC 3 cut(s) 209, 391, 1010
BslI CCNNNNNNNGG 4 cut(s) 188, 676, 677, 894
BsmAI GTCTC 2 cut(s) 114, 920
BsmFI GGGAC 3 cut(s) 209, 391, 1010
Bsp143I GATC 5 cut(s) 330, 480, 651, 957, 1047
BspACI CCGC 5 cut(s) 287, 473, 780, 845, 1020
BspCNI CTCAG 1 cut(s) 498
BspDI ATCGAT 1 cut(s) 479
BspHI TCATGA 1 cut(s) 108
BspLI GGNNCC 3 cut(s) 337, 589, 643
BspMAI CTGCAG 1 cut(s) 130
BspPI GGATC 1 cut(s) 1042
BsrBI CCGCTC 1 cut(s) 847
BsrDI GCAATG 1 cut(s) 238
BsrFI RCCGGY 1 cut(s) 289
BsrI ACTGG 3 cut(s) 223, 246, 580
BssAI RCCGGY 1 cut(s) 289
BssECI CCNNGG 4 cut(s) 450, 610, 754, 1054
BssMI GATC 5 cut(s) 330, 480, 651, 957, 1047
BssT1I CCWWGG 4 cut(s) 450, 610, 754, 1054
Bst2UI CCWGG 1 cut(s) 646
Bst4CI ACNGT 1 cut(s) 521
BstAPI GCANNNNNTGC 1 cut(s) 74
BstBAI YACGTR 1 cut(s) 988
BstC8I GCNNGC 3 cut(s) 291, 447, 460
BstDEI CTNAG 2 cut(s) 299, 506
BstF5I GGATG 5 cut(s) 356, 496, 591, 731, 1006
BstKTI GATC 5 cut(s) 333, 483, 654, 960, 1050
BstMAI GTCTC 2 cut(s) 114, 920
BstMBI GATC 5 cut(s) 330, 480, 651, 957, 1047
BstMCI CGRYCG 1 cut(s) 960
BstMWI GCNNNNNNNGC 5 cut(s) 74, 344, 353, 455, 863
BstNI CCWGG 1 cut(s) 646
BstSCI CCNGG 1 cut(s) 644
BstSFI CTRYAG 1 cut(s) 126
BstV1I GCAGC 9 cut(s) 35, 38, 340, 389, 402, 424, 474, 679, 794
BstV2I GAAGAC 1 cut(s) 598
Bsu15I ATCGAT 1 cut(s) 479
BsuTUI ATCGAT 1 cut(s) 479
BtsCI GGATG 5 cut(s) 356, 496, 591, 731, 1006
BtsI GCAGTG 2 cut(s) 255, 935
BtsIMutI CAGTG 3 cut(s) 255, 526, 935
Cac8I GCNNGC 3 cut(s) 291, 447, 460
CaiI CAGNNNCTG 1 cut(s) 415
CciI TCATGA 1 cut(s) 108
Cfr10I RCCGGY 1 cut(s) 289
ClaI ATCGAT 1 cut(s) 479
CseI GACGC 2 cut(s) 718, 991
CviAII CATG 3 cut(s) 109, 392, 716
DdeI CTNAG 2 cut(s) 299, 506
DpnI GATC 5 cut(s) 332, 482, 653, 959, 1049
DpnII GATC 5 cut(s) 330, 480, 651, 957, 1047
EciI GGCGGA 1 cut(s) 276
Eco130I CCWWGG 4 cut(s) 450, 610, 754, 1054
Eco57I CTGAAG 3 cut(s) 333, 515, 1035
EcoRII CCWGG 1 cut(s) 644
EcoT14I CCWWGG 4 cut(s) 450, 610, 754, 1054
EcoT22I ATGCAT 1 cut(s) 1045
ErhI CCWWGG 4 cut(s) 450, 610, 754, 1054
FaeI CATG 3 cut(s) 112, 395, 719
FaiI YATR 8 cut(s) 75, 110, 189, 393, 543, 585, 717, 852
FaqI GGGAC 3 cut(s) 209, 391, 1010
FatI CATG 3 cut(s) 108, 391, 715
FblI GTMKAC 1 cut(s) 567
FokI GGATG 5 cut(s) 343, 503, 578, 738, 993
FspBI CTAG 2 cut(s) 735, 1034
GsaI CCCAGC 2 cut(s) 412, 872
HapII CCGG 6 cut(s) 101, 290, 360, 513, 689, 810
HgaI GACGC 2 cut(s) 718, 991
Hin1II CATG 3 cut(s) 112, 395, 719
HincII GTYRAC 3 cut(s) 87, 547, 559
HindII GTYRAC 3 cut(s) 87, 547, 559
HindIII AAGCTT 1 cut(s) 443
HinfI GANTC 5 cut(s) 83, 122, 738, 882, 979
HpaI GTTAAC 1 cut(s) 559
HpaII CCGG 6 cut(s) 101, 290, 360, 513, 689, 810
HphI GGTGA 2 cut(s) 691, 877
Hpy166II GTNNAC 5 cut(s) 87, 276, 547, 559, 568
Hpy188I TCNGA 6 cut(s) 330, 335, 534, 748, 962, 1015
Hpy188III TCNNGA 3 cut(s) 16, 109, 575
Hpy8I GTNNAC 5 cut(s) 87, 276, 547, 559, 568
Hpy99I CGWCG 1 cut(s) 734
HpyAV CCTTC 3 cut(s) 186, 335, 695
HpyCH4III ACNGT 1 cut(s) 521
HpyCH4IV ACGT 2 cut(s) 113, 987
HpyCH4V TGCA 9 cut(s) 77, 128, 231, 356, 395, 418, 695, 945, 1043
HpyF10VI GCNNNNNNNGC 5 cut(s) 74, 344, 353, 455, 863
HpyF3I CTNAG 2 cut(s) 299, 506
HpySE526I ACGT 2 cut(s) 113, 987
Hsp92II CATG 3 cut(s) 112, 395, 719
KroI GCCGGC 1 cut(s) 289
KroNI GCCGGC 1 cut(s) 291
KspAI GTTAAC 1 cut(s) 559
Kzo9I GATC 5 cut(s) 330, 480, 651, 957, 1047
LmnI GCTCC 4 cut(s) 65, 335, 593, 641
Lsp1109I GCAGC 9 cut(s) 35, 38, 340, 389, 402, 424, 474, 679, 794
LweI GCATC 9 cut(s) 320, 365, 481, 485, 716, 739, 844, 1030, 1068
MaeI CTAG 2 cut(s) 735, 1034
MaeII ACGT 2 cut(s) 113, 987
MaeIII GTNAC 3 cut(s) 362, 521, 658
MalI GATC 5 cut(s) 332, 482, 653, 959, 1049
MbiI CCGCTC 1 cut(s) 847
MboI GATC 5 cut(s) 330, 480, 651, 957, 1047
MboII GAAGA 5 cut(s) 521, 598, 661, 781, 820
MluCI AATT 5 cut(s) 70, 175, 535, 874, 918
MlyI GAGTC 3 cut(s) 92, 116, 973
MnlI CCTC 9 cut(s) 164, 192, 216, 673, 681, 888, 893, 1061, 1076
Mph1103I ATGCAT 1 cut(s) 1045
MreI CGCCGGCG 1 cut(s) 289
MroNI GCCGGC 1 cut(s) 289
MroXI GAANNNNTTC 1 cut(s) 967
MseI TTAA 6 cut(s) 386, 558, 621, 762, 816, 861
MslI CAYNNNNRTG 1 cut(s) 940
MspA1I CMGCKG 2 cut(s) 353, 415
MspI CCGG 6 cut(s) 101, 290, 360, 513, 689, 810
MspR9I CCNGG 1 cut(s) 646
MvaI CCWGG 1 cut(s) 646
MwoI GCNNNNNNNGC 5 cut(s) 74, 344, 353, 455, 863
NaeI GCCGGC 1 cut(s) 291
NdeII GATC 5 cut(s) 330, 480, 651, 957, 1047
NgoMIV GCCGGC 1 cut(s) 289
NlaIII CATG 3 cut(s) 112, 395, 719
NlaIV GGNNCC 3 cut(s) 337, 589, 643
NmuCI GTSAC 2 cut(s) 521, 658
NsiI ATGCAT 1 cut(s) 1045
OliI CACNNNNGTG 1 cut(s) 940
PagI TCATGA 1 cut(s) 108
PcsI WCGNNNNNNNCGW 2 cut(s) 828, 885
PdiI GCCGGC 1 cut(s) 291
PdmI GAANNNNTTC 1 cut(s) 967
PfeI GAWTC 2 cut(s) 738, 882
PflFI GACNNNGTC 1 cut(s) 1001
Ple19I CGATCG 1 cut(s) 960
PleI GAGTC 3 cut(s) 91, 116, 973
PpsI GAGTC 3 cut(s) 91, 116, 973
Ppu21I YACGTR 1 cut(s) 988
Psp6I CCWGG 1 cut(s) 644
PspFI CCCAGC 2 cut(s) 408, 868
PspGI CCWGG 1 cut(s) 644
PspN4I GGNNCC 3 cut(s) 337, 589, 643
PstI CTGCAG 1 cut(s) 130
PstNI CAGNNNCTG 1 cut(s) 415
PsyI GACNNNGTC 1 cut(s) 1001
PvuI CGATCG 1 cut(s) 960
PvuII CAGCTG 2 cut(s) 353, 415
RseI CAYNNNNRTG 1 cut(s) 940
SaqAI TTAA 6 cut(s) 386, 558, 621, 762, 816, 861
Sau3AI GATC 5 cut(s) 330, 480, 651, 957, 1047
SchI GAGTC 3 cut(s) 92, 116, 973
ScrFI CCNGG 1 cut(s) 646
SfaNI GCATC 9 cut(s) 320, 365, 481, 485, 716, 739, 844, 1030, 1068
SfcI CTRYAG 1 cut(s) 126
SgrAI CRCCGGYG 1 cut(s) 289
SmiMI CAYNNNNRTG 1 cut(s) 940
SmlI CTYRAG 1 cut(s) 16
SmoI CTYRAG 1 cut(s) 16
Sse9I AATT 5 cut(s) 70, 175, 535, 874, 918
SsiI CCGC 5 cut(s) 287, 473, 780, 845, 1020
SspI AATATT 1 cut(s) 422
SspMI CTAG 2 cut(s) 735, 1034
StyD4I CCNGG 1 cut(s) 644
StyI CCWWGG 4 cut(s) 450, 610, 754, 1054
TaaI ACNGT 1 cut(s) 521
TaiI ACGT 2 cut(s) 116, 990
TaqI TCGA 3 cut(s) 479, 831, 956
TasI AATT 5 cut(s) 70, 175, 535, 874, 918
TauI GCSGC 3 cut(s) 476, 847, 1022
TfiI GAWTC 2 cut(s) 738, 882
Tru1I TTAA 6 cut(s) 386, 558, 621, 762, 816, 861
Tru9I TTAA 6 cut(s) 386, 558, 621, 762, 816, 861
TscAI CASTG 3 cut(s) 262, 526, 942
TseFI GTSAC 2 cut(s) 521, 658
TseI GCWGC 9 cut(s) 23, 26, 353, 377, 412, 415, 462, 692, 782
Tsp45I GTSAC 2 cut(s) 521, 658
TspDTI ATGAA 1 cut(s) 384
TspGWI ACGGA 3 cut(s) 263, 894, 999
TspRI CASTG 3 cut(s) 262, 526, 942
Tth111I GACNNNGTC 1 cut(s) 1001
XcmI CCANNNNNNNNNTGG 1 cut(s) 215
XmiI GTMKAC 1 cut(s) 567
XmnI GAANNNNTTC 1 cut(s) 967
XspI CTAG 2 cut(s) 735, 1034
Zsp2I ATGCAT 1 cut(s) 1045
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.