RchiOBHm_Chr2g0141171

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0005825
Physical Location & Seq
Reverse (-)
58728964 .. 58731855
2892 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51150

Sequence Viewer

Length: 1074 bp
ATGGGTAGCCAAACCATGAGAGCAGCTTTGTTTATGGTTTTTGTATTTGCCTTTGTAATTATTGGTGGCAATGCACAAGAATCAACCACTCTTGTTCCAGCCATCTTCACATTTGGTGATTCTGCAGTAGATGTGGGAAACAATGACTACCTCCCAACAATTTTCAAGGCCAATTACCCTCCTTATGGAAGAGACTTTAAGAACCATCAAGCTACTGGGAGGTTTTGCAATGGAAAACTAGCCACTGATATCACTGCTGATACTCTGGGCTTCACAACTTATCCACCTGCATATCTTAGCCCTCAGGCATCAGGGAAGAACCTTCTCATTGGAGCCAACTTTGCTTCTGCTGCATCCGGTTATGATGAAAAGGCAGCATACTTGAATCATGCAATCACCTTGCCCCAACAGTTACAGTACTTCAAGGAATACCAGGGTAAGCTAAACAAGATTGCAGGTAGTAAGAAAGCAGCAACAATTATCAAGGGTGCACTATACTTATTGAGCGCTGGCAGTAGTGACTTTGTTCAGAACTACTATGTCAATCCTTTTCTTAACAAACTCTACACTCCTGACCAGTATGGTGACATCCTCGTTGGTGCTTTCACAAGCTTTATTACGGGTGTGTATGGTTTGGGAGCAAGGAAAATAGGTGTGACATCACTCCCTCCATTGGGTTGCCTTCCGGCTGCAAGAACCTTATTTGGATTCCATGAGCCAGGCTGTGTCGCCAGACTCAACACTGATGCTCAAGCATTCAATAAGAAGATAAACAACGCAGCAAACACTCTCCAAAAGAAACTTCCTGGTCTTACAGTGGTCATCTTCGACATCTACAAGCCTCTGTTTGATGTTGTTAAGTCTCCATCAAACTATGGCTTTGCGGAAGCAAGGAGAGGTTGCTGTGGCACTGGGATTGTAGAGACGACATCATTGCTGTGCAATCCAAAGTCAATAGGAACTTGTGCTAATGCAACTCAGTATGTGTTCTGGGACAGCGTTCATCCATCTGAAGCTGCTAATCAAGTTCTTGCTGATGCATTGTTAGTCCAAGGTATCGGCCTCATTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0048046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

357

Amino Acids

38.27

Weight (kDa)

8.81

Isoelectric Point (pI)

20.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 35 - 348 2.3e-33 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 295
Acc36I ACCTGC 2 cut(s) 295, 446
AciI CCGC 1 cut(s) 884
AcuI CTGAAG 1 cut(s) 1032
AfaI GTAC 1 cut(s) 419
AfeI AGCGCT 1 cut(s) 508
AfiI CCNNNNNNNGG 3 cut(s) 185, 673, 674
AgsI TTSAA 4 cut(s) 166, 385, 424, 760
AjnI CCWGG 3 cut(s) 432, 718, 805
AjuI GAANNNNNNNTTGG 2 cut(s) 786, 818
AluBI AGCT 5 cut(s) 26, 212, 442, 612, 1016
AluI AGCT 5 cut(s) 26, 212, 442, 612, 1016
Alw21I GWGCWC 1 cut(s) 493
Alw26I GTCTC 3 cut(s) 186, 867, 917
Alw44I GTGCAC 1 cut(s) 489
Aor51HI AGCGCT 1 cut(s) 508
AoxI GGCC 2 cut(s) 168, 1060
ApaLI GTGCAC 1 cut(s) 489
ApeKI GCWGC 7 cut(s) 23, 350, 374, 470, 689, 779, 1016
AspLEI GCGC 1 cut(s) 509
AsuHPI GGTGA 3 cut(s) 128, 388, 596
AxyI CCTNAGG 1 cut(s) 303
BaeGI GKGCMC 1 cut(s) 493
Bbv12I GWGCWC 1 cut(s) 493
BbvI GCAGC 7 cut(s) 35, 337, 386, 482, 676, 791, 1003
BccI CCATC 4 cut(s) 110, 213, 874, 1015
BcgI CGANNNNNNTGC 2 cut(s) 916, 950
BciT130I CCWGG 3 cut(s) 434, 720, 807
BcoDI GTCTC 3 cut(s) 186, 867, 917
BfaI CTAG 1 cut(s) 239
BfmI CTRYAG 1 cut(s) 123
BfoI RGCGCY 1 cut(s) 510
BfuAI ACCTGC 2 cut(s) 295, 446
BisI GCNGC 7 cut(s) 24, 351, 375, 471, 690, 780, 1017
BlsI GCNGC 7 cut(s) 25, 352, 376, 472, 691, 781, 1018
BmcAI AGTACT 1 cut(s) 419
Bme1390I CCNGG 3 cut(s) 434, 720, 807
BmiI GGNNCC 1 cut(s) 334
BmrFI CCNGG 3 cut(s) 434, 720, 807
BmrI ACTGGG 2 cut(s) 225, 921
BmsI GCATC 4 cut(s) 317, 362, 736, 1027
BmuI ACTGGG 2 cut(s) 225, 921
BpuEI CTTGAG 1 cut(s) 735
BsaJI CCNNGG 2 cut(s) 433, 1051
BsaWI WCCGGW 1 cut(s) 356
Bsc4I CCNNNNNNNGG 3 cut(s) 185, 673, 674
Bse1I ACTGG 3 cut(s) 220, 577, 916
Bse21I CCTNAGG 1 cut(s) 303
Bse3DI GCAATG 3 cut(s) 76, 235, 932
BseBI CCWGG 3 cut(s) 434, 720, 807
BseDI CCNNGG 2 cut(s) 433, 1051
BseGI GGATG 3 cut(s) 353, 588, 1003
BseLI CCNNNNNNNGG 3 cut(s) 185, 673, 674
BseMI GCAATG 3 cut(s) 76, 235, 932
BseMII CTCAG 2 cut(s) 317, 992
BseNI ACTGG 3 cut(s) 220, 577, 916
BseSI GKGCMC 1 cut(s) 493
BseXI GCAGC 7 cut(s) 35, 337, 386, 482, 676, 791, 1003
BshFI GGCC 2 cut(s) 170, 1062
BsiHKAI GWGCWC 1 cut(s) 493
BsiSI CCGG 2 cut(s) 357, 686
BslFI GGGAC 1 cut(s) 1007
BslI CCNNNNNNNGG 3 cut(s) 185, 673, 674
BsmAI GTCTC 3 cut(s) 186, 867, 917
BsmBI CGTCTC 1 cut(s) 917
BsmFI GGGAC 1 cut(s) 1007
BsmI GAATGC 1 cut(s) 755
BsnI GGCC 2 cut(s) 170, 1062
Bsp1286I GDGCHC 1 cut(s) 493
BspACI CCGC 1 cut(s) 884
BspANI GGCC 2 cut(s) 170, 1062
BspCNI CTCAG 2 cut(s) 316, 991
BspHI TCATGA 1 cut(s) 1070
BspLI GGNNCC 1 cut(s) 334
BspMAI CTGCAG 1 cut(s) 127
BspMI ACCTGC 2 cut(s) 295, 446
BsrDI GCAATG 3 cut(s) 76, 235, 932
BsrI ACTGG 3 cut(s) 220, 577, 916
BssECI CCNNGG 2 cut(s) 433, 1051
BssT1I CCWWGG 1 cut(s) 1051
Bst2UI CCWGG 3 cut(s) 434, 720, 807
Bst4CI ACNGT 3 cut(s) 411, 417, 817
Bst6I CTCTTC 1 cut(s) 184
BstC8I GCNNGC 1 cut(s) 511
BstDEI CTNAG 3 cut(s) 296, 303, 978
BstF5I GGATG 3 cut(s) 353, 588, 1003
BstH2I RGCGCY 1 cut(s) 510
BstHHI GCGC 1 cut(s) 509
BstMAI GTCTC 3 cut(s) 186, 867, 917
BstMWI GCNNNNNNNGC 2 cut(s) 341, 350
BstNI CCWGG 3 cut(s) 434, 720, 807
BstSCI CCNGG 3 cut(s) 432, 718, 805
BstSFI CTRYAG 1 cut(s) 123
BstSLI GKGCMC 1 cut(s) 493
BstV1I GCAGC 7 cut(s) 35, 337, 386, 482, 676, 791, 1003
Bsu36I CCTNAGG 1 cut(s) 303
BsuRI GGCC 2 cut(s) 170, 1062
BtsCI GGATG 3 cut(s) 353, 588, 1003
BtsI GCAGTG 1 cut(s) 252
BtsIMutI CAGTG 5 cut(s) 243, 252, 741, 822, 909
BveI ACCTGC 2 cut(s) 295, 446
Cac8I GCNNGC 1 cut(s) 511
CciI TCATGA 1 cut(s) 1070
CfoI GCGC 1 cut(s) 509
Csp6I GTAC 1 cut(s) 418
CviAII CATG 4 cut(s) 16, 389, 713, 1071
CviQI GTAC 1 cut(s) 418
DdeI CTNAG 3 cut(s) 296, 303, 978
Eam1104I CTCTTC 1 cut(s) 184
EarI CTCTTC 1 cut(s) 184
Eco130I CCWWGG 1 cut(s) 1051
Eco32I GATATC 1 cut(s) 250
Eco47III AGCGCT 1 cut(s) 508
Eco57I CTGAAG 1 cut(s) 1032
Eco81I CCTNAGG 1 cut(s) 303
EcoRII CCWGG 3 cut(s) 432, 718, 805
EcoRV GATATC 1 cut(s) 250
EcoT14I CCWWGG 1 cut(s) 1051
EcoT22I ATGCAT 1 cut(s) 1042
ErhI CCWWGG 1 cut(s) 1051
Esp3I CGTCTC 1 cut(s) 917
FaeI CATG 4 cut(s) 19, 392, 716, 1074
FaqI GGGAC 1 cut(s) 1007
FatI CATG 4 cut(s) 15, 388, 712, 1070
Fnu4HI GCNGC 7 cut(s) 24, 351, 375, 471, 690, 780, 1017
FokI GGATG 3 cut(s) 340, 575, 990
Fsp4HI GCNGC 7 cut(s) 24, 351, 375, 471, 690, 780, 1017
FspBI CTAG 1 cut(s) 239
GlaI GCGC 1 cut(s) 508
GluI GCNGC 7 cut(s) 24, 351, 375, 471, 690, 780, 1017
HaeII RGCGCY 1 cut(s) 510
HaeIII GGCC 2 cut(s) 170, 1062
HapII CCGG 2 cut(s) 357, 686
HhaI GCGC 1 cut(s) 509
Hin1II CATG 4 cut(s) 19, 392, 716, 1074
Hin6I GCGC 1 cut(s) 507
HinP1I GCGC 1 cut(s) 507
HindIII AAGCTT 1 cut(s) 610
HinfI GANTC 5 cut(s) 80, 119, 385, 708, 735
HpaII CCGG 2 cut(s) 357, 686
HphI GGTGA 3 cut(s) 128, 388, 596
Hpy166II GTNNAC 1 cut(s) 491
Hpy188I TCNGA 2 cut(s) 531, 1012
Hpy188III TCNNGA 2 cut(s) 572, 1071
Hpy8I GTNNAC 1 cut(s) 491
HpyAV CCTTC 2 cut(s) 332, 692
HpyCH4III ACNGT 3 cut(s) 411, 417, 817
HpyF10VI GCNNNNNNNGC 2 cut(s) 341, 350
HpyF3I CTNAG 3 cut(s) 296, 303, 978
Hsp92II CATG 4 cut(s) 19, 392, 716, 1074
HspAI GCGC 1 cut(s) 507
LmnI GCTCC 2 cut(s) 332, 638
Lsp1109I GCAGC 7 cut(s) 35, 337, 386, 482, 676, 791, 1003
LweI GCATC 4 cut(s) 317, 362, 736, 1027
MaeI CTAG 1 cut(s) 239
MaeIII GTNAC 4 cut(s) 411, 518, 584, 655
MboII GAAGA 5 cut(s) 97, 201, 328, 778, 817
MhlI GDGCHC 1 cut(s) 493
MluCI AATT 4 cut(s) 57, 159, 172, 477
MlyI GAGTC 1 cut(s) 729
MnlI CCTC 9 cut(s) 161, 189, 213, 312, 602, 678, 852, 890, 1073
Mph1103I ATGCAT 1 cut(s) 1042
MseI TTAA 3 cut(s) 198, 555, 858
MslI CAYNNNNRTG 1 cut(s) 937
MspI CCGG 2 cut(s) 357, 686
MspR9I CCNGG 3 cut(s) 434, 720, 807
Mva1269I GAATGC 1 cut(s) 755
MvaI CCWGG 3 cut(s) 434, 720, 807
MwoI GCNNNNNNNGC 2 cut(s) 341, 350
NlaIII CATG 4 cut(s) 19, 392, 716, 1074
NlaIV GGNNCC 1 cut(s) 334
NmuCI GTSAC 3 cut(s) 518, 584, 655
NsiI ATGCAT 1 cut(s) 1042
PagI TCATGA 1 cut(s) 1070
PaqCI CACCTGC 1 cut(s) 295
PctI GAATGC 1 cut(s) 755
PfeI GAWTC 4 cut(s) 80, 119, 385, 708
PkrI GCNGC 7 cut(s) 25, 352, 376, 472, 691, 781, 1018
PleI GAGTC 1 cut(s) 729
PpsI GAGTC 1 cut(s) 729
Psp6I CCWGG 3 cut(s) 432, 718, 805
PspGI CCWGG 3 cut(s) 432, 718, 805
PspN4I GGNNCC 1 cut(s) 334
PstI CTGCAG 1 cut(s) 127
RsaI GTAC 1 cut(s) 419
RsaNI GTAC 1 cut(s) 418
RseI CAYNNNNRTG 1 cut(s) 937
SaqAI TTAA 3 cut(s) 198, 555, 858
SatI GCNGC 7 cut(s) 24, 351, 375, 471, 690, 780, 1017
ScaI AGTACT 1 cut(s) 419
SchI GAGTC 1 cut(s) 729
ScrFI CCNGG 3 cut(s) 434, 720, 807
SduI GDGCHC 1 cut(s) 493
SfaNI GCATC 4 cut(s) 317, 362, 736, 1027
SfcI CTRYAG 1 cut(s) 123
SmiMI CAYNNNNRTG 1 cut(s) 937
SmlI CTYRAG 1 cut(s) 750
SmoI CTYRAG 1 cut(s) 750
Sse9I AATT 4 cut(s) 57, 159, 172, 477
SsiI CCGC 1 cut(s) 884
SspMI CTAG 1 cut(s) 239
StyD4I CCNGG 3 cut(s) 432, 718, 805
StyI CCWWGG 1 cut(s) 1051
TaaI ACNGT 3 cut(s) 411, 417, 817
TaqI TCGA 1 cut(s) 828
TasI AATT 4 cut(s) 57, 159, 172, 477
TatI WGTACW 1 cut(s) 417
TfiI GAWTC 4 cut(s) 80, 119, 385, 708
Tru1I TTAA 3 cut(s) 198, 555, 858
Tru9I TTAA 3 cut(s) 198, 555, 858
TscAI CASTG 5 cut(s) 250, 259, 748, 822, 916
TseFI GTSAC 3 cut(s) 518, 584, 655
TseI GCWGC 7 cut(s) 23, 350, 374, 470, 689, 779, 1016
Tsp45I GTSAC 3 cut(s) 518, 584, 655
TspDTI ATGAA 3 cut(s) 381, 992, 1059
TspRI CASTG 5 cut(s) 250, 259, 748, 822, 916
VneI GTGCAC 1 cut(s) 489
XcmI CCANNNNNNNNNTGG 1 cut(s) 212
XspI CTAG 1 cut(s) 239
ZrmI AGTACT 1 cut(s) 419
Zsp2I ATGCAT 1 cut(s) 1042
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.