FvH4_6g32640

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005825
Physical Location & Seq
Reverse (-)
25670166 .. 25673569
3404 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g32640.t1

Sequence Viewer

Length: 1074 bp
ATGGGTAGCCAAACTATGAGAGCATCTTTGGTTCTGGTTTTTGTGTTTGCCTTTGTAATGATTGGTAGCAATGCACAAGAATCAACCACCCTTGTTCCAGCCATCTTCACATTTGGTGATTCTGCAGTAGATGTGGGAAACAATGACTACCTCCCAACAATTTTCAAGGCCAACTATCCTCCTTATGGAAGAGACTTTGTGAACCATAAAGCTACCGGGAGGTTTTGCAATGGGAAACTAGCCACTGATATCACTGCTGATACTCTGGGATTCAAACTTTATCCAGCTGCATATCTTAGCCCCCAGGCATCAGGGAAGAACCTTCTGATTGGAGCCAACTTTGCTTCTGCTGCATCCGGTTATGATGAGAAGGCAGCATACTTGAACCATGCAATCTCTTTGCCCCAACAGTTAGAATACTTCAAGGAATACCAGGGTAAGCTAAACAAGGTTGCAGGTAGTAAGAAAGCAGCAACAATCATCAAGGGTGCACTATACTTATTAAGTGCTGGAAATAGTGACTTTCTTCAGAACTACTATGTCAATCCTTTTGTTAACAAAGTCTACACTCCTGACCAATATGGTGACATCCTCATTGGTGCTTTCACAAGCTTCATCACGAATGTGTATGGTTTGGGAGCAAGGAAAATCGGTGTGACCTCACTCCCTCCATTGGGTTGCCTTCCAGCTGCAATTACTTTATTTGGATTCCATGAGCCAGGCTGCGTCGCCAGAATCAACACTGATGCGCAAGCATTCAATAAGAAGTTAAACAACGCAGCAAATAGTCTCCAAAAGAAACTTCCTGGTCTTACAGTTGTCATCTTCGACATCTATAAGCCTCTATATGATGTTGTTAAGTCTCCATCAAACTATGGCTTTGCGGAAGCAAGGAGAGGATGTTGTGGTACTGGGATTGTAGAGACAACATCATTGCTGTGCAATCCAAAGTCTGTAGGAACTTGTGCTAATGCGACTCAGTACGTGTTCTGGGACAGTGTTCATCCATCTGAAGCTGCTAATCAAGTTCTTGCTGATGCTCTGATTGTCCAAGGTATCGCCCTCATTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0048046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

358

Amino Acids

38.28

Weight (kDa)

8.67

Isoelectric Point (pI)

19.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 35 - 348 3.4e-34 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 750
Acc36I ACCTGC 1 cut(s) 446
AccI GTMKAC 1 cut(s) 564
AciI CCGC 1 cut(s) 884
AcuI CTGAAG 2 cut(s) 512, 1032
AfaI GTAC 2 cut(s) 910, 983
AfiI CCNNNNNNNGG 3 cut(s) 185, 673, 674
AflIII ACRYGT 1 cut(s) 984
AgsI TTSAA 5 cut(s) 166, 274, 385, 424, 760
AjnI CCWGG 4 cut(s) 303, 432, 718, 805
AjuI GAANNNNNNNTTGG 2 cut(s) 786, 818
AleI CACNNNNGTG 1 cut(s) 623
AluBI AGCT 6 cut(s) 212, 287, 442, 612, 689, 1016
AluI AGCT 6 cut(s) 212, 287, 442, 612, 689, 1016
Alw21I GWGCWC 1 cut(s) 493
Alw26I GTCTC 4 cut(s) 186, 794, 867, 917
Alw44I GTGCAC 1 cut(s) 489
AoxI GGCC 1 cut(s) 168
ApaLI GTGCAC 1 cut(s) 489
ApeKI GCWGC 8 cut(s) 287, 350, 374, 470, 689, 723, 779, 1016
AspLEI GCGC 1 cut(s) 751
AsuC2I CCSGG 1 cut(s) 217
AsuHPI GGTGA 2 cut(s) 128, 596
BaeGI GKGCMC 1 cut(s) 493
BarI GAAGNNNNNNTAC 2 cut(s) 362, 394
Bbv12I GWGCWC 1 cut(s) 493
BbvI GCAGC 8 cut(s) 274, 337, 386, 482, 676, 710, 791, 1003
BccI CCATC 3 cut(s) 110, 874, 1015
BciT130I CCWGG 4 cut(s) 305, 434, 720, 807
BcnI CCSGG 1 cut(s) 217
BcoDI GTCTC 4 cut(s) 186, 794, 867, 917
BfaI CTAG 1 cut(s) 239
BfmI CTRYAG 2 cut(s) 123, 954
BfuAI ACCTGC 1 cut(s) 446
BisI GCNGC 8 cut(s) 288, 351, 375, 471, 690, 724, 780, 1017
BlsI GCNGC 8 cut(s) 289, 352, 376, 472, 691, 725, 781, 1018
Bme1390I CCNGG 5 cut(s) 217, 305, 434, 720, 807
BmiI GGNNCC 1 cut(s) 334
BmrFI CCNGG 5 cut(s) 217, 305, 434, 720, 807
BmrI ACTGGG 1 cut(s) 921
BmsI GCATC 5 cut(s) 32, 317, 362, 736, 1027
BmuI ACTGGG 1 cut(s) 921
BpuMI CCSGG 1 cut(s) 217
BsaAI YACGTR 1 cut(s) 985
BsaJI CCNNGG 3 cut(s) 303, 433, 1051
BsaWI WCCGGW 1 cut(s) 356
BsaXI ACNNNNNCTCC 2 cut(s) 886, 916
Bsc4I CCNNNNNNNGG 3 cut(s) 185, 673, 674
Bse1I ACTGG 1 cut(s) 916
Bse3DI GCAATG 3 cut(s) 76, 235, 932
BseBI CCWGG 4 cut(s) 305, 434, 720, 807
BseDI CCNNGG 3 cut(s) 303, 433, 1051
BseGI GGATG 4 cut(s) 353, 588, 905, 1003
BseLI CCNNNNNNNGG 3 cut(s) 185, 673, 674
BseMI GCAATG 3 cut(s) 76, 235, 932
BseMII CTCAG 1 cut(s) 992
BseNI ACTGG 1 cut(s) 916
BseSI GKGCMC 1 cut(s) 493
BseXI GCAGC 8 cut(s) 274, 337, 386, 482, 676, 710, 791, 1003
BshFI GGCC 1 cut(s) 170
BsiHKAI GWGCWC 1 cut(s) 493
BsiSI CCGG 2 cut(s) 216, 357
BslFI GGGAC 1 cut(s) 1007
BslI CCNNNNNNNGG 3 cut(s) 185, 673, 674
BsmAI GTCTC 4 cut(s) 186, 794, 867, 917
BsmFI GGGAC 1 cut(s) 1007
BsmI GAATGC 1 cut(s) 755
BsnI GGCC 1 cut(s) 170
Bsp1286I GDGCHC 1 cut(s) 493
BspACI CCGC 1 cut(s) 884
BspANI GGCC 1 cut(s) 170
BspCNI CTCAG 1 cut(s) 991
BspLI GGNNCC 1 cut(s) 334
BspMAI CTGCAG 1 cut(s) 127
BspMI ACCTGC 1 cut(s) 446
BsrDI GCAATG 3 cut(s) 76, 235, 932
BsrI ACTGG 1 cut(s) 916
BssECI CCNNGG 3 cut(s) 303, 433, 1051
BssT1I CCWWGG 1 cut(s) 1051
Bst2UI CCWGG 4 cut(s) 305, 434, 720, 807
Bst4CI ACNGT 3 cut(s) 411, 817, 998
Bst6I CTCTTC 1 cut(s) 184
BstBAI YACGTR 1 cut(s) 985
BstC8I GCNNGC 1 cut(s) 753
BstDEI CTNAG 2 cut(s) 296, 978
BstF5I GGATG 4 cut(s) 353, 588, 905, 1003
BstHHI GCGC 1 cut(s) 751
BstMAI GTCTC 4 cut(s) 186, 794, 867, 917
BstMWI GCNNNNNNNGC 2 cut(s) 341, 350
BstNI CCWGG 4 cut(s) 305, 434, 720, 807
BstSCI CCNGG 5 cut(s) 215, 303, 432, 718, 805
BstSFI CTRYAG 2 cut(s) 123, 954
BstSLI GKGCMC 1 cut(s) 493
BstV1I GCAGC 8 cut(s) 274, 337, 386, 482, 676, 710, 791, 1003
BsuRI GGCC 1 cut(s) 170
BtsCI GGATG 4 cut(s) 353, 588, 905, 1003
BtsI GCAGTG 1 cut(s) 252
BtsIMutI CAGTG 4 cut(s) 243, 252, 741, 1003
BveI ACCTGC 1 cut(s) 446
Cac8I GCNNGC 1 cut(s) 753
CfoI GCGC 1 cut(s) 751
CseI GACGC 1 cut(s) 715
Csp6I GTAC 2 cut(s) 909, 982
CviAII CATG 3 cut(s) 389, 713, 1071
CviQI GTAC 2 cut(s) 909, 982
DdeI CTNAG 2 cut(s) 296, 978
Eam1104I CTCTTC 1 cut(s) 184
EarI CTCTTC 1 cut(s) 184
Eco130I CCWWGG 1 cut(s) 1051
Eco32I GATATC 1 cut(s) 250
Eco57I CTGAAG 2 cut(s) 512, 1032
EcoRII CCWGG 4 cut(s) 303, 432, 718, 805
EcoRV GATATC 1 cut(s) 250
EcoT14I CCWWGG 1 cut(s) 1051
ErhI CCWWGG 1 cut(s) 1051
FaeI CATG 3 cut(s) 392, 716, 1074
FaqI GGGAC 1 cut(s) 1007
FatI CATG 3 cut(s) 388, 712, 1070
FblI GTMKAC 1 cut(s) 564
Fnu4HI GCNGC 8 cut(s) 288, 351, 375, 471, 690, 724, 780, 1017
FokI GGATG 4 cut(s) 340, 575, 912, 990
Fsp4HI GCNGC 8 cut(s) 288, 351, 375, 471, 690, 724, 780, 1017
FspBI CTAG 1 cut(s) 239
FspI TGCGCA 1 cut(s) 750
GlaI GCGC 1 cut(s) 750
GluI GCNGC 8 cut(s) 288, 351, 375, 471, 690, 724, 780, 1017
HaeIII GGCC 1 cut(s) 170
HapII CCGG 2 cut(s) 216, 357
HgaI GACGC 1 cut(s) 715
HhaI GCGC 1 cut(s) 751
Hin1II CATG 3 cut(s) 392, 716, 1074
Hin6I GCGC 1 cut(s) 749
HinP1I GCGC 1 cut(s) 749
HincII GTYRAC 1 cut(s) 556
HindII GTYRAC 1 cut(s) 556
HindIII AAGCTT 1 cut(s) 610
HinfI GANTC 6 cut(s) 80, 119, 270, 708, 735, 976
HpaI GTTAAC 1 cut(s) 556
HpaII CCGG 2 cut(s) 216, 357
HphI GGTGA 2 cut(s) 128, 596
Hpy166II GTNNAC 4 cut(s) 202, 491, 556, 565
Hpy188I TCNGA 4 cut(s) 327, 531, 1012, 1044
Hpy188III TCNNGA 2 cut(s) 572, 619
Hpy8I GTNNAC 4 cut(s) 202, 491, 556, 565
Hpy99I CGWCG 1 cut(s) 731
HpyAV CCTTC 3 cut(s) 332, 364, 692
HpyCH4III ACNGT 3 cut(s) 411, 817, 998
HpyCH4IV ACGT 1 cut(s) 984
HpyF10VI GCNNNNNNNGC 2 cut(s) 341, 350
HpyF3I CTNAG 2 cut(s) 296, 978
HpySE526I ACGT 1 cut(s) 984
Hsp92II CATG 3 cut(s) 392, 716, 1074
HspAI GCGC 1 cut(s) 749
KspAI GTTAAC 1 cut(s) 556
LmnI GCTCC 2 cut(s) 332, 638
Lsp1109I GCAGC 8 cut(s) 274, 337, 386, 482, 676, 710, 791, 1003
LweI GCATC 5 cut(s) 32, 317, 362, 736, 1027
MaeI CTAG 1 cut(s) 239
MaeII ACGT 1 cut(s) 984
MaeIII GTNAC 3 cut(s) 518, 584, 655
MboII GAAGA 5 cut(s) 97, 201, 328, 518, 817
MhlI GDGCHC 1 cut(s) 493
MluCI AATT 2 cut(s) 159, 693
MlyI GAGTC 1 cut(s) 970
MnlI CCTC 9 cut(s) 161, 189, 213, 602, 670, 678, 852, 890, 1073
MseI TTAA 4 cut(s) 503, 555, 770, 858
MslI CAYNNNNRTG 2 cut(s) 623, 937
MspA1I CMGCKG 2 cut(s) 287, 689
MspI CCGG 2 cut(s) 216, 357
MspR9I CCNGG 5 cut(s) 217, 305, 434, 720, 807
Mva1269I GAATGC 1 cut(s) 755
MvaI CCWGG 4 cut(s) 305, 434, 720, 807
MwoI GCNNNNNNNGC 2 cut(s) 341, 350
NciI CCSGG 1 cut(s) 217
NlaIII CATG 3 cut(s) 392, 716, 1074
NlaIV GGNNCC 1 cut(s) 334
NmuCI GTSAC 3 cut(s) 518, 584, 655
NsbI TGCGCA 1 cut(s) 750
OliI CACNNNNGTG 1 cut(s) 623
PctI GAATGC 1 cut(s) 755
PfeI GAWTC 5 cut(s) 80, 119, 270, 708, 735
PkrI GCNGC 8 cut(s) 289, 352, 376, 472, 691, 725, 781, 1018
PleI GAGTC 1 cut(s) 970
PpsI GAGTC 1 cut(s) 970
Ppu21I YACGTR 1 cut(s) 985
Psp6I CCWGG 4 cut(s) 303, 432, 718, 805
PspGI CCWGG 4 cut(s) 303, 432, 718, 805
PspN4I GGNNCC 1 cut(s) 334
PstI CTGCAG 1 cut(s) 127
PvuII CAGCTG 2 cut(s) 287, 689
RsaI GTAC 2 cut(s) 910, 983
RsaNI GTAC 2 cut(s) 909, 982
RseI CAYNNNNRTG 2 cut(s) 623, 937
SaqAI TTAA 4 cut(s) 503, 555, 770, 858
SatI GCNGC 8 cut(s) 288, 351, 375, 471, 690, 724, 780, 1017
SchI GAGTC 1 cut(s) 970
ScrFI CCNGG 5 cut(s) 217, 305, 434, 720, 807
SduI GDGCHC 1 cut(s) 493
SfaNI GCATC 5 cut(s) 32, 317, 362, 736, 1027
SfcI CTRYAG 2 cut(s) 123, 954
SmiMI CAYNNNNRTG 2 cut(s) 623, 937
Sse9I AATT 2 cut(s) 159, 693
SsiI CCGC 1 cut(s) 884
SspMI CTAG 1 cut(s) 239
StyD4I CCNGG 5 cut(s) 215, 303, 432, 718, 805
StyI CCWWGG 1 cut(s) 1051
TaaI ACNGT 3 cut(s) 411, 817, 998
TaiI ACGT 1 cut(s) 987
TaqI TCGA 1 cut(s) 828
TasI AATT 2 cut(s) 159, 693
TfiI GAWTC 5 cut(s) 80, 119, 270, 708, 735
Tru1I TTAA 4 cut(s) 503, 555, 770, 858
Tru9I TTAA 4 cut(s) 503, 555, 770, 858
TscAI CASTG 4 cut(s) 250, 259, 748, 1003
TseFI GTSAC 3 cut(s) 518, 584, 655
TseI GCWGC 8 cut(s) 287, 350, 374, 470, 689, 723, 779, 1016
Tsp45I GTSAC 3 cut(s) 518, 584, 655
TspDTI ATGAA 2 cut(s) 604, 992
TspRI CASTG 4 cut(s) 250, 259, 748, 1003
VneI GTGCAC 1 cut(s) 489
XmiI GTMKAC 1 cut(s) 564
XspI CTAG 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.