MD09G1199700.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005825
Physical Location & Seq
Forward (+)
18307840 .. 18310470
2631 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1199700.v1.1.491

Sequence Viewer

Length: 1167 bp
ATGCGTAACCAGAAACTTCAAAAGGGCCATTTTCAAAAGCACTTTATAAGTTCCAATTTCACAACACAAAGCACTTCTGTTTTTCACAACATGGAAAGCCATTTCTTGAGAGCAGCAGCTTTTGGTTTGGTTTTGGCTTTTGCGTTTGTGATTGGAGGAAATTATGCACAAGAGTCAACCACACTTGTTCCGGCAATCATGACGTTTGGAGACTCTGCAGTTGATGTGGGCAACAATGACTACCTCCCTACCATTTTCAAGGCTAATTACCCTCCTTATGGAAGGGACTTTGTGAGCCATCAGCCTACTGGGAGGTTTTGCAATGGCAAACTGGCAACTGACATCACTGCCGACACTCTGGGGTTCACTACTTATCCGCCGGCGTATCTTAGCCCCCAAGCATCAGGGAAAAACCTTCTGATCGGAGCCAACTTCGCTTCAGCTGCATCGGGTTACGATGAAAAGGCAGCAACCTTAAACCATGCAATCCCATTGTCCCAGCAGCTGCAATATTATAAGGAATACCAGACAAAGCTCGCCAAGGTAGCAGGCAGCAAGAAAGCGGCGTCGATCATCAAGGATGCTCTCTATTTACTGAGTGCCGGAAACAGTGACTTTCTTCAGAATTACTATGTCAACCCTTTTGTTAACAAAGTCTACACTCCTGACCAGTATGGCTCCATCCTTGTTGGTGTCTTCCAAGGCTTCGTTAAGGATTTGTACCACCTGGGAGCCAGGAAGATCGGTGTGACCTCACTCCCTCCATTGGGTTGCCTTCCGGCTGCAATCACCTTATTTGGAAACCATGAGCAAGGATGTGTCGCTAGAATCAACTCCGATGCCCAAGGGTTTAACAAGAAGATAAACTCCGCAGCGGGAAATCTCCAAAAGCAACTTTCCGGTCTTAACATCGTCATCTTCGACATTTACAAGCCGCTCTATGATGTCATTAAAGCCCCAGCAAATTACGGATTCACTGAAGCGAGGAGAGGTTGCTGTGGAACGGGAATTGTAGAGACAACATCACTGCTGTGCAATCCCAAGTCGATTGGAACTTGTTCTAATGCGACTCAATACGTGTTTTGGGACAGCGTCCATCCGTCTCAAGCCGCTAACCAAGTTCTTGCCGATGCATTGATCACCCAAGGCATCTCCCTCATTGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0048046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

389

Amino Acids

41.94

Weight (kDa)

8.88

Isoelectric Point (pI)

25.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 66 - 379 3.7e-33 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 47, 516
AccBSI CCGCTC 1 cut(s) 937
AccI GTMKAC 1 cut(s) 657
AciI CCGC 6 cut(s) 377, 563, 870, 875, 935, 1110
AcuI CTGAAG 3 cut(s) 423, 605, 999
AcyI GRCGYC 1 cut(s) 566
AfaI GTAC 1 cut(s) 722
AfiI CCNNNNNNNGG 3 cut(s) 278, 766, 767
AflIII ACRYGT 1 cut(s) 1077
AgsI TTSAA 3 cut(s) 20, 35, 259
AjnI CCWGG 2 cut(s) 726, 734
AleI CACNNNNGTG 1 cut(s) 1030
AluBI AGCT 4 cut(s) 119, 443, 505, 535
AluI AGCT 4 cut(s) 119, 443, 505, 535
Alw26I GTCTC 3 cut(s) 204, 1010, 1107
AlwNI CAGNNNCTG 1 cut(s) 505
AoxI GGCC 1 cut(s) 25
ApeKI GCWGC 9 cut(s) 113, 116, 443, 467, 502, 505, 552, 782, 872
Asp700I GAANNNNTTC 1 cut(s) 1057
AspS9I GGNCC 1 cut(s) 25
AsuHPI GGTGA 2 cut(s) 781, 1132
BbsI GAAGAC 1 cut(s) 688
BbvI GCAGC 9 cut(s) 125, 128, 430, 479, 492, 514, 564, 769, 884
BccI CCATC 3 cut(s) 306, 689, 1104
BciT130I CCWGG 2 cut(s) 728, 736
BclI TGATCA 1 cut(s) 1137
BcoDI GTCTC 3 cut(s) 204, 1010, 1107
BfaI CTAG 1 cut(s) 825
BfmI CTRYAG 1 cut(s) 216
Bme1390I CCNGG 2 cut(s) 728, 736
BmgT120I GGNCC 1 cut(s) 25
BmiI GGNNCC 3 cut(s) 427, 679, 733
BmrFI CCNGG 2 cut(s) 728, 736
BmrI ACTGGG 1 cut(s) 318
BmsI GCATC 6 cut(s) 410, 455, 571, 829, 1120, 1158
BmuI ACTGGG 1 cut(s) 318
BpiI GAAGAC 1 cut(s) 688
BpuEI CTTGAG 2 cut(s) 127, 1089
BsaAI YACGTR 1 cut(s) 1078
BsaHI GRCGYC 1 cut(s) 566
BsaJI CCNNGG 5 cut(s) 540, 700, 727, 844, 1144
BsaWI WCCGGW 1 cut(s) 899
Bsc4I CCNNNNNNNGG 3 cut(s) 278, 766, 767
Bse118I RCCGGY 1 cut(s) 379
Bse1I ACTGG 3 cut(s) 313, 336, 670
Bse3DI GCAATG 1 cut(s) 328
BseBI CCWGG 2 cut(s) 728, 736
BseDI CCNNGG 5 cut(s) 540, 700, 727, 844, 1144
BseGI GGATG 4 cut(s) 586, 681, 821, 1096
BseLI CCNNNNNNNGG 3 cut(s) 278, 766, 767
BseMI GCAATG 1 cut(s) 328
BseMII CTCAG 1 cut(s) 587
BseNI ACTGG 3 cut(s) 313, 336, 670
BseRI GAGGAG 1 cut(s) 1000
BseXI GCAGC 9 cut(s) 125, 128, 430, 479, 492, 514, 564, 769, 884
BseYI CCCAGC 2 cut(s) 498, 958
BshFI GGCC 1 cut(s) 27
BsiSI CCGG 5 cut(s) 191, 380, 603, 779, 900
BslFI GGGAC 3 cut(s) 299, 481, 1100
BslI CCNNNNNNNGG 3 cut(s) 278, 766, 767
BsmAI GTCTC 3 cut(s) 204, 1010, 1107
BsmBI CGTCTC 1 cut(s) 1107
BsmFI GGGAC 3 cut(s) 299, 481, 1100
BsnI GGCC 1 cut(s) 27
Bsp143I GATC 4 cut(s) 420, 570, 741, 1137
BspACI CCGC 6 cut(s) 377, 563, 870, 875, 935, 1110
BspANI GGCC 1 cut(s) 27
BspCNI CTCAG 1 cut(s) 588
BspHI TCATGA 1 cut(s) 198
BspLI GGNNCC 3 cut(s) 427, 679, 733
BspMAI CTGCAG 1 cut(s) 220
BsrBI CCGCTC 1 cut(s) 937
BsrDI GCAATG 1 cut(s) 328
BsrFI RCCGGY 1 cut(s) 379
BsrI ACTGG 3 cut(s) 313, 336, 670
BssAI RCCGGY 1 cut(s) 379
BssECI CCNNGG 5 cut(s) 540, 700, 727, 844, 1144
BssMI GATC 4 cut(s) 420, 570, 741, 1137
BssNI GRCGYC 1 cut(s) 566
BssT1I CCWWGG 4 cut(s) 540, 700, 844, 1144
Bst2UI CCWGG 2 cut(s) 728, 736
Bst4CI ACNGT 1 cut(s) 611
BstACI GRCGYC 1 cut(s) 566
BstBAI YACGTR 1 cut(s) 1078
BstC8I GCNNGC 3 cut(s) 381, 537, 550
BstDEI CTNAG 2 cut(s) 389, 596
BstF5I GGATG 4 cut(s) 586, 681, 821, 1096
BstKTI GATC 4 cut(s) 423, 573, 744, 1140
BstMAI GTCTC 3 cut(s) 204, 1010, 1107
BstMBI GATC 4 cut(s) 420, 570, 741, 1137
BstMWI GCNNNNNNNGC 3 cut(s) 434, 443, 545
BstNI CCWGG 2 cut(s) 728, 736
BstSCI CCNGG 2 cut(s) 726, 734
BstSFI CTRYAG 1 cut(s) 216
BstV1I GCAGC 9 cut(s) 125, 128, 430, 479, 492, 514, 564, 769, 884
BstV2I GAAGAC 1 cut(s) 688
BsuRI GGCC 1 cut(s) 27
BtsCI GGATG 4 cut(s) 586, 681, 821, 1096
BtsI GCAGTG 2 cut(s) 345, 1025
BtsIMutI CAGTG 4 cut(s) 345, 616, 975, 1025
Cac8I GCNNGC 3 cut(s) 381, 537, 550
CaiI CAGNNNCTG 1 cut(s) 505
CciI TCATGA 1 cut(s) 198
Cfr10I RCCGGY 1 cut(s) 379
Cfr13I GGNCC 1 cut(s) 25
CseI GACGC 2 cut(s) 555, 1081
Csp6I GTAC 1 cut(s) 721
CviAII CATG 4 cut(s) 91, 199, 482, 806
CviQI GTAC 1 cut(s) 721
DdeI CTNAG 2 cut(s) 389, 596
DpnI GATC 4 cut(s) 422, 572, 743, 1139
DpnII GATC 4 cut(s) 420, 570, 741, 1137
EciI GGCGGA 1 cut(s) 366
Eco130I CCWWGG 4 cut(s) 540, 700, 844, 1144
Eco57I CTGAAG 3 cut(s) 423, 605, 999
EcoRII CCWGG 2 cut(s) 726, 734
EcoT14I CCWWGG 4 cut(s) 540, 700, 844, 1144
EcoT22I ATGCAT 1 cut(s) 1135
ErhI CCWWGG 4 cut(s) 540, 700, 844, 1144
Esp3I CGTCTC 1 cut(s) 1107
FaeI CATG 4 cut(s) 94, 202, 485, 809
FaqI GGGAC 3 cut(s) 299, 481, 1100
FatI CATG 4 cut(s) 90, 198, 481, 805
FauI CCCGC 1 cut(s) 868
FbaI TGATCA 1 cut(s) 1137
FblI GTMKAC 1 cut(s) 657
FokI GGATG 4 cut(s) 593, 668, 828, 1083
FspBI CTAG 1 cut(s) 825
GsaI CCCAGC 2 cut(s) 502, 962
HaeIII GGCC 1 cut(s) 27
HapII CCGG 5 cut(s) 191, 380, 603, 779, 900
HgaI GACGC 2 cut(s) 555, 1081
Hin1I GRCGYC 1 cut(s) 566
Hin1II CATG 4 cut(s) 94, 202, 485, 809
HincII GTYRAC 3 cut(s) 177, 637, 649
HindII GTYRAC 3 cut(s) 177, 637, 649
HinfI GANTC 5 cut(s) 173, 212, 828, 972, 1069
HpaI GTTAAC 1 cut(s) 649
HpaII CCGG 5 cut(s) 191, 380, 603, 779, 900
HphI GGTGA 2 cut(s) 781, 1132
Hpy166II GTNNAC 5 cut(s) 177, 366, 637, 649, 658
Hpy188I TCNGA 4 cut(s) 420, 425, 624, 838
Hpy188III TCNNGA 3 cut(s) 106, 199, 665
Hpy8I GTNNAC 5 cut(s) 177, 366, 637, 649, 658
Hpy99I CGWCG 1 cut(s) 571
HpyAV CCTTC 3 cut(s) 276, 425, 785
HpyCH4III ACNGT 1 cut(s) 611
HpyCH4IV ACGT 2 cut(s) 203, 1077
HpyCH4V TGCA 9 cut(s) 167, 218, 321, 446, 485, 508, 785, 1035, 1133
HpyF10VI GCNNNNNNNGC 3 cut(s) 434, 443, 545
HpyF3I CTNAG 2 cut(s) 389, 596
HpySE526I ACGT 2 cut(s) 203, 1077
Hsp92I GRCGYC 1 cut(s) 566
Hsp92II CATG 4 cut(s) 94, 202, 485, 809
KroI GCCGGC 1 cut(s) 379
KroNI GCCGGC 1 cut(s) 381
Ksp22I TGATCA 1 cut(s) 1137
KspAI GTTAAC 1 cut(s) 649
Kzo9I GATC 4 cut(s) 420, 570, 741, 1137
LmnI GCTCC 3 cut(s) 425, 683, 731
Lsp1109I GCAGC 9 cut(s) 125, 128, 430, 479, 492, 514, 564, 769, 884
LweI GCATC 6 cut(s) 410, 455, 571, 829, 1120, 1158
MaeI CTAG 1 cut(s) 825
MaeII ACGT 2 cut(s) 203, 1077
MaeIII GTNAC 4 cut(s) 5, 452, 611, 748
MalI GATC 4 cut(s) 422, 572, 743, 1139
MbiI CCGCTC 1 cut(s) 937
MboI GATC 4 cut(s) 420, 570, 741, 1137
MboII GAAGA 5 cut(s) 611, 688, 751, 871, 910
MluCI AATT 6 cut(s) 55, 160, 265, 625, 964, 1008
MlyI GAGTC 3 cut(s) 182, 206, 1063
MnlI CCTC 9 cut(s) 149, 254, 282, 306, 763, 771, 978, 983, 1166
Mph1103I ATGCAT 1 cut(s) 1135
MreI CGCCGGCG 1 cut(s) 379
MroNI GCCGGC 1 cut(s) 379
MroXI GAANNNNTTC 1 cut(s) 1057
MseI TTAA 6 cut(s) 476, 648, 711, 852, 906, 951
MslI CAYNNNNRTG 1 cut(s) 1030
MspA1I CMGCKG 3 cut(s) 443, 505, 875
MspI CCGG 5 cut(s) 191, 380, 603, 779, 900
MspR9I CCNGG 2 cut(s) 728, 736
MvaI CCWGG 2 cut(s) 728, 736
MwoI GCNNNNNNNGC 3 cut(s) 434, 443, 545
NaeI GCCGGC 1 cut(s) 381
NdeII GATC 4 cut(s) 420, 570, 741, 1137
NgoMIV GCCGGC 1 cut(s) 379
NlaIII CATG 4 cut(s) 94, 202, 485, 809
NlaIV GGNNCC 3 cut(s) 427, 679, 733
NmuCI GTSAC 2 cut(s) 611, 748
NsiI ATGCAT 1 cut(s) 1135
OliI CACNNNNGTG 1 cut(s) 1030
PagI TCATGA 1 cut(s) 198
PcsI WCGNNNNNNNCGW 1 cut(s) 918
PdiI GCCGGC 1 cut(s) 381
PdmI GAANNNNTTC 1 cut(s) 1057
PfeI GAWTC 2 cut(s) 828, 972
PflFI GACNNNGTC 1 cut(s) 1091
PleI GAGTC 3 cut(s) 181, 206, 1063
PpsI GAGTC 3 cut(s) 181, 206, 1063
Ppu21I YACGTR 1 cut(s) 1078
PsiI TTATAA 2 cut(s) 47, 516
Psp6I CCWGG 2 cut(s) 726, 734
PspFI CCCAGC 2 cut(s) 498, 958
PspGI CCWGG 2 cut(s) 726, 734
PspN4I GGNNCC 3 cut(s) 427, 679, 733
PspPI GGNCC 1 cut(s) 25
PstI CTGCAG 1 cut(s) 220
PstNI CAGNNNCTG 1 cut(s) 505
PsyI GACNNNGTC 1 cut(s) 1091
PvuII CAGCTG 2 cut(s) 443, 505
RsaI GTAC 1 cut(s) 722
RsaNI GTAC 1 cut(s) 721
RseI CAYNNNNRTG 1 cut(s) 1030
SaqAI TTAA 6 cut(s) 476, 648, 711, 852, 906, 951
Sau3AI GATC 4 cut(s) 420, 570, 741, 1137
Sau96I GGNCC 1 cut(s) 25
SchI GAGTC 3 cut(s) 182, 206, 1063
ScrFI CCNGG 2 cut(s) 728, 736
SfaNI GCATC 6 cut(s) 410, 455, 571, 829, 1120, 1158
SfcI CTRYAG 1 cut(s) 216
SgrAI CRCCGGYG 1 cut(s) 379
SmiMI CAYNNNNRTG 1 cut(s) 1030
SmlI CTYRAG 2 cut(s) 106, 1104
SmoI CTYRAG 2 cut(s) 106, 1104
Sse9I AATT 6 cut(s) 55, 160, 265, 625, 964, 1008
SsiI CCGC 6 cut(s) 377, 563, 870, 875, 935, 1110
SspI AATATT 1 cut(s) 512
SspMI CTAG 1 cut(s) 825
StyD4I CCNGG 2 cut(s) 726, 734
StyI CCWWGG 4 cut(s) 540, 700, 844, 1144
TaaI ACNGT 1 cut(s) 611
TaiI ACGT 2 cut(s) 206, 1080
TaqI TCGA 3 cut(s) 569, 921, 1046
TasI AATT 6 cut(s) 55, 160, 265, 625, 964, 1008
TauI GCSGC 3 cut(s) 566, 937, 1112
TfiI GAWTC 2 cut(s) 828, 972
Tru1I TTAA 6 cut(s) 476, 648, 711, 852, 906, 951
Tru9I TTAA 6 cut(s) 476, 648, 711, 852, 906, 951
TscAI CASTG 4 cut(s) 352, 616, 982, 1032
TseFI GTSAC 2 cut(s) 611, 748
TseI GCWGC 9 cut(s) 113, 116, 443, 467, 502, 505, 552, 782, 872
Tsp45I GTSAC 2 cut(s) 611, 748
TspDTI ATGAA 1 cut(s) 474
TspGWI ACGGA 2 cut(s) 984, 1089
TspRI CASTG 4 cut(s) 352, 616, 982, 1032
Tth111I GACNNNGTC 1 cut(s) 1091
XcmI CCANNNNNNNNNTGG 1 cut(s) 305
XmiI GTMKAC 1 cut(s) 657
XmnI GAANNNNTTC 1 cut(s) 1057
XspI CTAG 1 cut(s) 825
Zsp2I ATGCAT 1 cut(s) 1135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.