Rw0G019770

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00889
OG0005825
Physical Location & Seq
Forward (+)
132613 .. 139043
6431 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G019770.1

Sequence Viewer

Length: 1074 bp
ATGGGTAGCCAACCCATCAGAGCAGCTTTGGTTCTGGTTTTTGTATTTGCCTTTGCAATTATTGGTGGCAATGCACAAGAATCAACCACTCTTGTTCCAGCCATCATCACATTTGGTGATTCTGCAGTAGATGTGGGAAACAATGACTACCTCCTAACAATTTATAAGGCCAATTACCCTCCTTATGGAAGAGACTTTGTGAACCATCAAGCTACTGGGAGGTTTTGCAATGGAAAACTAGCCACCGATATCACTGCTGATACTCTGGGCTTCACAACTTATCCACCTGCATATCTTAGCCCTCAGGCGTCAGAGAAGAACCTTCTCATTGGAGCCAACTTTGCTTCCGCTGCATCCGGTTATGATCAAACGGCAGCAAACTTCAATCGTGCAATCTCTTTGCCCCGACAGTTACAATACTTTAAGGAATACCAGGGTAAGCTAAACAAGGTTGCAGGTAGTGAGAAAGCAGCAACAATTATCGAGGGTGCCCTATACTTATTGAGTGCTGGCAGTAGTGATTTTGTTCAGAACTACTATGTCAATCCTTTTGTTAACAAATTCTACACTCCTGACAAGTACGGTGACATCCTCGTTGGTGCTTTCACACGCTTTATTACGGATGTGTATGGTTTGGGAGCAAGGAAAATAGGTGTGACATCACTCCCTCCATTGGGTTGCCTTCCAGCTGCAAGAACCTTATTTGGACCCCATGAGCCAGGCTGTGTCGCCAGACTCAACACTGATGCTCAAGCATTCAATAAGAAGATAAATAACGCAGCAGAAACTCTCCAAAAGAAACTTCCTGGTCTTACACTGGTCATCTTCGACATCTACAAGCCTCTATTTGATGTTGTTGAGTCTCCATCAAACTATGGCTTTGCGGAAGCAAGGAGAGGTTGCTGTGGCACGGGGATTATAGAGACGACATCATTGCTGTGCAATTCAAAGTCAATAGGAACTTGTGCTAATGCAACTCAATATGTGTTCTGGGACAGCGTTCATCCATCTGAAGCTGCTAATCAATTTCTTGCTGATGCATTGATTGTCCAAGGGGTTCCCCTCATTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0048046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

357

Amino Acids

38.38

Weight (kDa)

6.81

Isoelectric Point (pI)

27.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 35 - 348 9.5e-31 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 165
AarI CACCTGC 1 cut(s) 295
Acc36I ACCTGC 2 cut(s) 295, 446
AccB1I GGYRCC 1 cut(s) 488
AciI CCGC 2 cut(s) 348, 884
AcsI RAATTY 1 cut(s) 560
AcuI CTGAAG 1 cut(s) 1032
AcyI GRCGYC 1 cut(s) 308
AfaI GTAC 1 cut(s) 581
AfiI CCNNNNNNNGG 3 cut(s) 185, 673, 674
AgsI TTSAA 3 cut(s) 385, 760, 948
AjnI CCWGG 3 cut(s) 432, 718, 805
AjuI GAANNNNNNNTTGG 2 cut(s) 786, 818
AluBI AGCT 5 cut(s) 26, 212, 442, 689, 1016
AluI AGCT 5 cut(s) 26, 212, 442, 689, 1016
Alw26I GTCTC 3 cut(s) 186, 867, 917
AoxI GGCC 1 cut(s) 168
ApeKI GCWGC 7 cut(s) 23, 350, 374, 470, 689, 779, 1016
ApoI RAATTY 1 cut(s) 560
AspS9I GGNCC 1 cut(s) 707
AsuHPI GGTGA 2 cut(s) 128, 596
AvaII GGWCC 1 cut(s) 707
AxyI CCTNAGG 1 cut(s) 303
BaeGI GKGCMC 1 cut(s) 493
BanI GGYRCC 1 cut(s) 488
BbvI GCAGC 7 cut(s) 35, 337, 386, 482, 676, 791, 1003
BccI CCATC 5 cut(s) 23, 110, 213, 874, 1015
BceAI ACGGC 1 cut(s) 387
BcgI CGANNNNNNTGC 4 cut(s) 236, 270, 916, 950
BciT130I CCWGG 3 cut(s) 434, 720, 807
BclI TGATCA 1 cut(s) 364
BcoDI GTCTC 3 cut(s) 186, 867, 917
BfaI CTAG 1 cut(s) 239
BfmI CTRYAG 1 cut(s) 123
BfuAI ACCTGC 2 cut(s) 295, 446
BisI GCNGC 7 cut(s) 24, 351, 375, 471, 690, 780, 1017
BlsI GCNGC 7 cut(s) 25, 352, 376, 472, 691, 781, 1018
Bme1390I CCNGG 3 cut(s) 434, 720, 807
Bme18I GGWCC 1 cut(s) 707
BmgT120I GGNCC 1 cut(s) 707
BmiI GGNNCC 4 cut(s) 334, 490, 709, 1059
BmrFI CCNGG 3 cut(s) 434, 720, 807
BmrI ACTGGG 1 cut(s) 225
BmsI GCATC 3 cut(s) 362, 736, 1027
BmuI ACTGGG 1 cut(s) 225
BpuEI CTTGAG 1 cut(s) 735
BsaHI GRCGYC 1 cut(s) 308
BsaJI CCNNGG 2 cut(s) 433, 1051
BsaWI WCCGGW 1 cut(s) 356
Bsc4I CCNNNNNNNGG 3 cut(s) 185, 673, 674
Bse1I ACTGG 2 cut(s) 220, 822
Bse21I CCTNAGG 1 cut(s) 303
Bse3DI GCAATG 3 cut(s) 76, 235, 932
BseBI CCWGG 3 cut(s) 434, 720, 807
BseDI CCNNGG 2 cut(s) 433, 1051
BseGI GGATG 4 cut(s) 353, 588, 628, 1003
BseLI CCNNNNNNNGG 3 cut(s) 185, 673, 674
BseMI GCAATG 3 cut(s) 76, 235, 932
BseMII CTCAG 1 cut(s) 317
BseNI ACTGG 2 cut(s) 220, 822
BseSI GKGCMC 1 cut(s) 493
BseXI GCAGC 7 cut(s) 35, 337, 386, 482, 676, 791, 1003
BshFI GGCC 1 cut(s) 170
BshNI GGYRCC 1 cut(s) 488
BsiSI CCGG 1 cut(s) 357
BslFI GGGAC 1 cut(s) 1007
BslI CCNNNNNNNGG 3 cut(s) 185, 673, 674
BsmAI GTCTC 3 cut(s) 186, 867, 917
BsmBI CGTCTC 1 cut(s) 917
BsmFI GGGAC 1 cut(s) 1007
BsmI GAATGC 1 cut(s) 755
BsnI GGCC 1 cut(s) 170
Bsp1286I GDGCHC 1 cut(s) 493
Bsp143I GATC 1 cut(s) 364
BspACI CCGC 2 cut(s) 348, 884
BspANI GGCC 1 cut(s) 170
BspCNI CTCAG 1 cut(s) 316
BspHI TCATGA 1 cut(s) 1070
BspLI GGNNCC 4 cut(s) 334, 490, 709, 1059
BspMAI CTGCAG 1 cut(s) 127
BspMI ACCTGC 2 cut(s) 295, 446
BspT107I GGYRCC 1 cut(s) 488
BsrDI GCAATG 3 cut(s) 76, 235, 932
BsrI ACTGG 2 cut(s) 220, 822
BssECI CCNNGG 2 cut(s) 433, 1051
BssMI GATC 1 cut(s) 364
BssNI GRCGYC 1 cut(s) 308
BssT1I CCWWGG 1 cut(s) 1051
Bst2UI CCWGG 3 cut(s) 434, 720, 807
Bst4CI ACNGT 2 cut(s) 411, 584
Bst6I CTCTTC 1 cut(s) 184
BstACI GRCGYC 1 cut(s) 308
BstC8I GCNNGC 1 cut(s) 511
BstDEI CTNAG 2 cut(s) 296, 303
BstF5I GGATG 4 cut(s) 353, 588, 628, 1003
BstKTI GATC 1 cut(s) 367
BstMAI GTCTC 3 cut(s) 186, 867, 917
BstMBI GATC 1 cut(s) 364
BstMWI GCNNNNNNNGC 2 cut(s) 341, 350
BstNI CCWGG 3 cut(s) 434, 720, 807
BstSCI CCNGG 3 cut(s) 432, 718, 805
BstSFI CTRYAG 1 cut(s) 123
BstSLI GKGCMC 1 cut(s) 493
BstV1I GCAGC 7 cut(s) 35, 337, 386, 482, 676, 791, 1003
Bsu36I CCTNAGG 1 cut(s) 303
BsuRI GGCC 1 cut(s) 170
BtsCI GGATG 4 cut(s) 353, 588, 628, 1003
BtsI GCAGTG 1 cut(s) 252
BtsIMutI CAGTG 3 cut(s) 252, 741, 815
BveI ACCTGC 2 cut(s) 295, 446
Cac8I GCNNGC 1 cut(s) 511
CciI TCATGA 1 cut(s) 1070
Cfr13I GGNCC 1 cut(s) 707
CseI GACGC 1 cut(s) 297
Csp6I GTAC 1 cut(s) 580
CviAII CATG 2 cut(s) 713, 1071
CviQI GTAC 1 cut(s) 580
DdeI CTNAG 2 cut(s) 296, 303
DpnI GATC 1 cut(s) 366
DpnII GATC 1 cut(s) 364
Eam1104I CTCTTC 1 cut(s) 184
EarI CTCTTC 1 cut(s) 184
Eco130I CCWWGG 1 cut(s) 1051
Eco32I GATATC 1 cut(s) 250
Eco47I GGWCC 1 cut(s) 707
Eco57I CTGAAG 1 cut(s) 1032
Eco81I CCTNAGG 1 cut(s) 303
EcoRII CCWGG 3 cut(s) 432, 718, 805
EcoRV GATATC 1 cut(s) 250
EcoT14I CCWWGG 1 cut(s) 1051
EcoT22I ATGCAT 1 cut(s) 1042
ErhI CCWWGG 1 cut(s) 1051
Esp3I CGTCTC 1 cut(s) 917
FaeI CATG 2 cut(s) 716, 1074
FaqI GGGAC 1 cut(s) 1007
FatI CATG 2 cut(s) 712, 1070
FbaI TGATCA 1 cut(s) 364
Fnu4HI GCNGC 7 cut(s) 24, 351, 375, 471, 690, 780, 1017
FokI GGATG 4 cut(s) 340, 575, 635, 990
Fsp4HI GCNGC 7 cut(s) 24, 351, 375, 471, 690, 780, 1017
FspBI CTAG 1 cut(s) 239
GluI GCNGC 7 cut(s) 24, 351, 375, 471, 690, 780, 1017
HaeIII GGCC 1 cut(s) 170
HapII CCGG 1 cut(s) 357
HgaI GACGC 1 cut(s) 297
Hin1I GRCGYC 1 cut(s) 308
Hin1II CATG 2 cut(s) 716, 1074
HincII GTYRAC 1 cut(s) 556
HindII GTYRAC 1 cut(s) 556
HinfI GANTC 4 cut(s) 80, 119, 735, 860
HpaI GTTAAC 1 cut(s) 556
HpaII CCGG 1 cut(s) 357
HphI GGTGA 2 cut(s) 128, 596
Hpy166II GTNNAC 2 cut(s) 202, 556
Hpy188I TCNGA 4 cut(s) 20, 313, 531, 1012
Hpy188III TCNNGA 2 cut(s) 572, 1071
Hpy8I GTNNAC 2 cut(s) 202, 556
HpyAV CCTTC 2 cut(s) 332, 692
HpyCH4III ACNGT 2 cut(s) 411, 584
HpyF10VI GCNNNNNNNGC 2 cut(s) 341, 350
HpyF3I CTNAG 2 cut(s) 296, 303
Hsp92I GRCGYC 1 cut(s) 308
Hsp92II CATG 2 cut(s) 716, 1074
Ksp22I TGATCA 1 cut(s) 364
KspAI GTTAAC 1 cut(s) 556
Kzo9I GATC 1 cut(s) 364
LmnI GCTCC 2 cut(s) 332, 638
Lsp1109I GCAGC 7 cut(s) 35, 337, 386, 482, 676, 791, 1003
LweI GCATC 3 cut(s) 362, 736, 1027
MaeI CTAG 1 cut(s) 239
MaeIII GTNAC 3 cut(s) 411, 584, 655
MalI GATC 1 cut(s) 366
MboI GATC 1 cut(s) 364
MboII GAAGA 4 cut(s) 201, 328, 778, 817
MhlI GDGCHC 1 cut(s) 493
MluCI AATT 7 cut(s) 57, 159, 172, 477, 560, 943, 1025
MlyI GAGTC 2 cut(s) 729, 869
Mph1103I ATGCAT 1 cut(s) 1042
MseI TTAA 2 cut(s) 423, 555
MslI CAYNNNNRTG 1 cut(s) 937
MspA1I CMGCKG 2 cut(s) 350, 689
MspI CCGG 1 cut(s) 357
MspR9I CCNGG 3 cut(s) 434, 720, 807
Mva1269I GAATGC 1 cut(s) 755
MvaI CCWGG 3 cut(s) 434, 720, 807
MwoI GCNNNNNNNGC 2 cut(s) 341, 350
NdeII GATC 1 cut(s) 364
NlaIII CATG 2 cut(s) 716, 1074
NlaIV GGNNCC 4 cut(s) 334, 490, 709, 1059
NmuCI GTSAC 2 cut(s) 584, 655
NsiI ATGCAT 1 cut(s) 1042
PagI TCATGA 1 cut(s) 1070
PaqCI CACCTGC 1 cut(s) 295
PctI GAATGC 1 cut(s) 755
PfeI GAWTC 2 cut(s) 80, 119
PkrI GCNGC 7 cut(s) 25, 352, 376, 472, 691, 781, 1018
PleI GAGTC 2 cut(s) 729, 868
PpsI GAGTC 2 cut(s) 729, 868
PsiI TTATAA 1 cut(s) 165
Psp6I CCWGG 3 cut(s) 432, 718, 805
PspGI CCWGG 3 cut(s) 432, 718, 805
PspN4I GGNNCC 4 cut(s) 334, 490, 709, 1059
PspPI GGNCC 1 cut(s) 707
PstI CTGCAG 1 cut(s) 127
PvuII CAGCTG 1 cut(s) 689
RsaI GTAC 1 cut(s) 581
RsaNI GTAC 1 cut(s) 580
RseI CAYNNNNRTG 1 cut(s) 937
SaqAI TTAA 2 cut(s) 423, 555
SatI GCNGC 7 cut(s) 24, 351, 375, 471, 690, 780, 1017
Sau3AI GATC 1 cut(s) 364
Sau96I GGNCC 1 cut(s) 707
SchI GAGTC 2 cut(s) 729, 869
ScrFI CCNGG 3 cut(s) 434, 720, 807
SduI GDGCHC 1 cut(s) 493
SfaNI GCATC 3 cut(s) 362, 736, 1027
SfcI CTRYAG 1 cut(s) 123
SinI GGWCC 1 cut(s) 707
SmiMI CAYNNNNRTG 1 cut(s) 937
SmlI CTYRAG 1 cut(s) 750
SmoI CTYRAG 1 cut(s) 750
Sse9I AATT 7 cut(s) 57, 159, 172, 477, 560, 943, 1025
SsiI CCGC 2 cut(s) 348, 884
SspMI CTAG 1 cut(s) 239
StyD4I CCNGG 3 cut(s) 432, 718, 805
StyI CCWWGG 1 cut(s) 1051
TaaI ACNGT 2 cut(s) 411, 584
TaqI TCGA 2 cut(s) 483, 828
TasI AATT 7 cut(s) 57, 159, 172, 477, 560, 943, 1025
TfiI GAWTC 2 cut(s) 80, 119
Tru1I TTAA 2 cut(s) 423, 555
Tru9I TTAA 2 cut(s) 423, 555
TscAI CASTG 3 cut(s) 259, 748, 822
TseFI GTSAC 2 cut(s) 584, 655
TseI GCWGC 7 cut(s) 23, 350, 374, 470, 689, 779, 1016
Tsp45I GTSAC 2 cut(s) 584, 655
TspDTI ATGAA 2 cut(s) 992, 1059
TspGWI ACGGA 1 cut(s) 635
TspRI CASTG 3 cut(s) 259, 748, 822
VpaK11BI GGWCC 1 cut(s) 707
XapI RAATTY 1 cut(s) 560
XcmI CCANNNNNNNNNTGG 1 cut(s) 212
XspI CTAG 1 cut(s) 239
Zsp2I ATGCAT 1 cut(s) 1042
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.