AT3G16830

Topless-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0013792
Physical Location & Seq
Forward (+)
5731519 .. 5737854
6336 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G16830.1

Sequence Viewer

Length: 3396 bp
ATGTCGTCTTTGAGCAGAGAGTTAGTTTTCTTAATTCTCCAGTTCTTAGATGAGGAGAAGTTCAAGGAATCTGTTCACAAGCTAGAGCAAGAATCTGGCTTCTTCTTTAACATTAAGTACTTTGAAGAGAAAGCTCTTGCTGGAGAGTGGGATGAAGTGGAGAAGTATCTTTCTGGTTTCACTAAAGTCGATGATAATCGTTACTCTATGAAAATCTTCTTTGAGATTAGGAAGCAAAAGTATCTCGAAGCTCTAGATCGAAATGACAGAGCAAAAGCTGTTGAGATATTAGCCAAAGACTTGAAAGTTTTCGCTACATTTAACGAGGAGCTTTACAAGGAGATTACTCAGCTGCTTACTCTGGAGAATTTTAGGGAAAATGAGCAACTGTCGAAATATGGTGATACTAAATCTGCAAGGTCTATAATGTATACTGAGTTGAAGAAATTGATTGAGGCGAATCCTCTGTTTCGAGAGAAGCTAGCCTTCCCCAGTTTCAAAGCTTCTCGCCTGCGAACTTTGATCAATCAAAGCTTAAACTGGCAGCATCAGCTATGTAAAAATCCAAGGCCTAATCCTGACATCAAGACATTGTTCTTGGATCACTCTTGCTCACCATCAAACGGTGCTCGTGCACTCACTCCTGTCAATCTTCCAGTTGCTGCTGTTGCAAGACCTTCAAACTTTGTTCCTCTTGGAGTGCATGGTGGACCCTTTCAATCTAATCCTGCTCCGGCTCCTAATGCCAATGCTTTAGCTGGATGGATGGCAAACCCAAATCCTTCTTCATCAGTTCCATCTGGTGTTGTCGCTGCTTCCCCATTTCCTATGCAGCCAAGTCAAGTTAATGAGCTGAAACACCCTCGGGCACCGTCTAACTCTCTAGGATTAATGGATTATCAAAGTGCTGATCACGAGCAACTCATGAAACGCTTACGGTCTGCACAAACATCCAACGAGGTCACATATCCTGCTCATTCTCATCCCCCTGCGTCACTTGATGACCTCCCTAGAAATGTAGTGAGTACAATTCGCCAAGGGTCGGTGGTGATAAGCATGGATTTTCACCCTTCTCATCACACTTTGCTCGCTGTTGGTTGTTCAAGCGGCGAAGTCACCTTGTGGGAAGTTGGATCCAGAGAAAAGGTTGTCACAGAACCTTTCAAGATATGGAATATGGCGGCTTGTTCTGTGATCTTCCAGGGTTCTATAGTCAAAGAGCCATCGATATCCGTCACTCGTGTAGCATGGAGCCCAGATGGAAACTTGCTTGGGGTTTCTTTTACTAAACATCTGATCCATGTATATGCTTACCAAGGCTCAGACCTACGTCAACACCTCGAGATTGATGCTCATGTAGGCTGTGTGAATGACTTGGCGTTCGCCCACCCAAATAAGCAAATGTGTGTTGTGACTTGTGGGGATGACAAATTGATTAAGGTGTGGGATTTGAGTGGGAAGAAGCTCTTCACCTTTGAAGGGCACGAGGCACCAGTTTATTCCATTTGCCCTCACCAGAAGGAAAATATTCAGTTTATATTCTCAACCGCTCTTGATGGAAAGATCAAGGCGTGGCTCTATGATAATGTTGGTTCTCGGGTTGATTATGACGCACCGGGACAGTGGTGTACAACAATGCTCTACAGTGCTGATGGAAGCAGACTATTTTCTTGTGGGACGAGTAAAGAAGGAGACTCTTTCCTCGTCGAGTGGAATGAGAGTGAAGGTGCCCTAAAGAGGACATATTTAGGCTTTAGGAAGAAATCAGCTGGAGTTGTTCAGTTTGATACAACCCGTAACCGTTTCTTGGCTGTTGGTGAAGACAATCAAATAAAATTCTGGAACATGGACAATACAAATCTTTTGACAGTTGTTGAGGCTGAAGGTGGACTTCCGAATCTACCCCGATTGAGATTCAACAAGGATGGGAATCTTCTTGCAGTTACTACAGCAGATAATGGATTCAAGATCCTTGCAAATACTGATGGTCTCAGAACCTTAAGAGCATTTGAAGCGCGGTCTTTTGAAGCATCTAAAGCATCCATTGATATGAAGGTATCCACCTCCGCAATGGCTTCAAGCATCAGCCCCGCTATTGGTAAAATTGAACACATGGACGCAGGCTCTCCTGCAAGGCCAACCCCAATTCCTAATGGAATTGAGGCAATGTCAAGAACTATGGAAAAGCCAAGAAACTTGGACTCTGTTGATAAGTCTAAACCTTTAGAGCTCACAGAAATTGTTGATCCGACTCAGTGCCGACAAGTGACAATGCCAGATAGCAAGGACTCTGTTAGCAAGGTTGCTCGGCTTCTTTACACAAATTCTGGCGTTGGTGTTTTGGCTCTTGGATCAAATGGTGTTCAAAGACTATGGAAGTGGATCCGCAATGAGCAAAATCCAACTGGGAAGGCAACTGCTAGTGTAACTCCACAGCATTGGCAGCCAAACAGCGGTCTCCTAATGGCAAATGATGTTCCCGAGAATCCTGAAGGGAGTGTTCCGTGTATAGCACTTTCCAAGAATGACTCATACGTAATGTCAGCTTGTGGTGGAAAGGTTTCGTTATTCAATATGATGACTTTCAAAGTAATGACAACTTTCATGCCACCGCCACCAGCTTCAACTTTCTTGGCATTTCATCCTCAAGATAATAATATTATTGCAATCGGGATGGAGGACTCGAGTATACATATTTACAACGTCAGGGTAGATGAGGTGAAAACAAAGTTGAAAGGTCACCAGAAGCACATAACTGGTCTAGCATTCTCTACCGCGCTCAATATTCTTGTCTCTTCTGGTGCCGATGCTCAGCTCTTCTTCTGGACCGCTGACTCGTGGGAGAAAAAGAAATCTTCAGCGATACAGCTACCACCTGGCAAAGCACCAGTAGGCGACACACGAGTCCAATTCCACAACGACCAAATCCAGTTATTGGTTTCACATGAGACACAGCTCGCCATTTACGATGCTTCGAAGATGGAATGCATTCATAAGTGGGTGCCCCAAGAAGCTCTCTCTTCGCCCATAACCTCTGCATCTTACTCATGCAACAGCCAACTGGTTTACGCGTCATTCGCTGATGGAAACATTGCAGTCTTTGATGCTGAATCCTTGAGACTCAGATGCCGCATTGCCCCATCAGCCTACATGCCTCAACCTACACCAAACAGTGCACCGATTTTCCCACAGGTGATCACAGCTCATCCACAAGAACCGAATCAATTAGCGGTTGGACTGTCTGATGGTTCGGTTAAAGTGATTGAGCCATCAGAACTTTCTAGAAGATGGGGTGTTGGCGTCGCAGCTGGTTCAGATAAAGCAGGGACTGAAAATGGACGGCCGTCTAGTTCATCAGCGGCTAACAACTCAAGTTCGGATCAGATTCAAAGGTAA

Protein Analysis

1131

Amino Acids

124.76

Weight (kDa)

6.86

Isoelectric Point (pI)

44.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH_TPL PF17814 5 - 34 1.6e-12 LisH-like dimerisation domain
TPR1-like_2nd PF21889 37 - 161 1.3e-61 TPR1-like, CTLH-containing domain
zf_topless PF21359 164 - 204 1e-24 TOPLESS, zinc finger domain
WDR55 PF24796 343 - 656 2.2e-12 WDR55
Beta-prop_WDR3_2nd PF25172 349 - 562 4.5e-08 WDR3 second beta-propeller domain
Beta-prop_EML PF23409 350 - 606 5.6e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_RIG_2nd PF23775 352 - 499 6e-06 RIG second beta-propeller
Beta-prop_WDR3_1st PF25173 386 - 561 2e-14 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 388 - 528 1e-05 WDR36/Utp21 first beta-propeller
WD40_Prp19 PF24814 407 - 484 1.1e-06 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 409 - 559 3.2e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 410 - 622 9.1e-21 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 411 - 663 2.5e-21 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 462 - 557 1e-06 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 466 - 528 8.4e-08 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 828 - 945 2.9e-14 WDR3 first beta-propeller domain
WDR55 PF24796 828 - 943 3e-06 WDR55
WD40_CDC20-Fz PF24807 830 - 949 5e-11 CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 831 - 942 1.2e-07 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 832 - 1038 3.9e-19 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 832 - 953 1.2e-11 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd PF25172 834 - 954 3e-11 WDR3 second beta-propeller domain
WD40_WDHD1_1st PF24817 841 - 1038 2.2e-16 WDHD1 first WD40 domain
Beta-prop_IFT140_1st PF23383 847 - 950 1.3e-09 IFT140 first beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 851 - 943 9.1e-10 WDR36/Utp21 first beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 853 - 945 7.1e-07 WDR90/POC16, second beta-propeller
WD40_Prp19 PF24814 874 - 1038 2.6e-15 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 885 - 1041 1.8e-10 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 886 - 1039 2.9e-08 WDR36/Utp21 second beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013792)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G16830
fragaria_vesca FvH4_4g18910 FvH4_4g18910
malus_domestica MD13G1162100.v1.1 MD16G1161900.v1.1
prunus_persica Prupe.1G129400_v2.0.a1
pyrus_communis pycom13g13830
rosa_chinensis RchiOBHm_Chr4g0423811
rosa_laevigata RLG00000007530
rosa_multiflora Rmu_sc0003369.1_g000018
rosa_roxburghii Rroxscaffold_5G00365920
rosa_rugosa Rorug04G0190900
rosa_samantha Rh4AG250300 Rh4BG255600 Rh4CG263700 Rh4DG250000
rosa_wichuraiana Rw4G021430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2903
AccB1I GGYRCC 5 cut(s) 868, 1489, 1727, 2801, 3001
AccB7I CCANNNNNTGG 1 cut(s) 2935
AccBSI CCGCTC 1 cut(s) 1550
AccI GTMKAC 2 cut(s) 431, 2689
AccII CGCG 3 cut(s) 2017, 2777, 3071
AcoI YGGCCR 1 cut(s) 3341
AcsI RAATTY 3 cut(s) 367, 1835, 2323
AcuI CTGAAG 3 cut(s) 1902, 2511, 2841
AcyI GRCGYC 1 cut(s) 3300
AdeI CACNNNGTG 1 cut(s) 1122
AfaI GTAC 3 cut(s) 119, 1027, 1630
AfiI CCNNNNNNNGG 8 cut(s) 623, 1042, 1479, 1737, 1807, 2096, 2453, 2935
AflII CTTAAG 1 cut(s) 1999
AflIII ACRYGT 1 cut(s) 3069
AjnI CCWGG 2 cut(s) 1200, 2875
AjuI GAANNNNNNNTTGG 2 cut(s) 769, 801
Alw21I GWGCWC 4 cut(s) 631, 637, 2232, 3178
Alw26I GTCTC 6 cut(s) 1686, 1994, 2462, 2797, 2942, 3112
Alw44I GTGCAC 2 cut(s) 633, 3174
AlwNI CAGNNNCTG 2 cut(s) 662, 3329
Ama87I CYCGRG 5 cut(s) 864, 1340, 1596, 2480, 2683
AoxI GGCC 3 cut(s) 569, 2135, 3341
ApaLI GTGCAC 2 cut(s) 633, 3174
ApeKI GCWGC 7 cut(s) 352, 544, 662, 812, 832, 2443, 3305
ApoI RAATTY 3 cut(s) 367, 1835, 2323
ArsI GACNNNNNNTTYG 2 cut(s) 1364, 1396
AseI ATTAAT 1 cut(s) 890
Asp700I GAANNNNTTC 7 cut(s) 72, 215, 308, 1466, 1527, 2560, 2988
AspLEI GCGC 2 cut(s) 2017, 2779
AspS9I GGNCC 2 cut(s) 710, 2826
AsuC2I CCSGG 1 cut(s) 1617
AsuII TTCGAA 1 cut(s) 2975
AsuNHI GCTAGC 1 cut(s) 481
AvaI CYCGRG 5 cut(s) 864, 1340, 1596, 2480, 2683
AvaII GGWCC 2 cut(s) 710, 2826
BaeGI GKGCMC 6 cut(s) 637, 871, 1485, 1732, 3006, 3178
BamHI GGATCC 2 cut(s) 1133, 2382
BanI GGYRCC 5 cut(s) 868, 1489, 1727, 2801, 3001
BanII GRGCYC 2 cut(s) 1256, 2232
BauI CACGAG 6 cut(s) 630, 914, 1239, 1484, 2836, 2901
BbsI GAAGAC 1 cut(s) 1827
Bbv12I GWGCWC 4 cut(s) 631, 637, 2232, 3178
BbvI GCAGC 7 cut(s) 339, 556, 649, 799, 844, 2455, 3317
BceAI ACGGC 2 cut(s) 3328, 3356
BcgI CGANNNNNNTGC 2 cut(s) 1331, 1365
BciT130I CCWGG 2 cut(s) 1202, 2877
BciVI GTATCC 1 cut(s) 2068
BclI TGATCA 3 cut(s) 522, 910, 3195
BcnI CCSGG 1 cut(s) 1617
BcoDI GTCTC 6 cut(s) 1686, 1994, 2462, 2797, 2942, 3112
BfaI CTAG 9 cut(s) 83, 254, 482, 884, 1011, 2421, 2762, 3282, 3348
BfmI CTRYAG 3 cut(s) 1209, 1642, 1947
BfrI CTTAAG 1 cut(s) 1999
BfuI GTATCC 1 cut(s) 2068
BlpI GCTNAGC 1 cut(s) 2811
BmcAI AGTACT 1 cut(s) 119
Bme1390I CCNGG 3 cut(s) 1202, 1617, 2877
Bme18I GGWCC 2 cut(s) 710, 2826
BmeT110I CYCGRG 5 cut(s) 864, 1340, 1596, 2480, 2683
BmgT120I GGNCC 2 cut(s) 710, 2826
BmrFI CCNGG 3 cut(s) 1202, 1617, 2877
BmrI ACTGGG 2 cut(s) 486, 2415
BmtI GCTAGC 1 cut(s) 485
BmuI ACTGGG 2 cut(s) 486, 2415
BoxI GACNNNNGTC 2 cut(s) 1329, 3343
BpiI GAAGAC 1 cut(s) 1827
BpmI CTGGAG 4 cut(s) 23, 162, 383, 1791
Bpu1102I GCTNAGC 1 cut(s) 2811
Bpu14I TTCGAA 1 cut(s) 2975
BpuEI CTTGAG 3 cut(s) 2631, 3136, 3355
BpuMI CCSGG 1 cut(s) 1617
Bsa29I ATCGAT 1 cut(s) 1226
BsaAI YACGTR 1 cut(s) 2536
BsaBI GATNNNNATC 2 cut(s) 195, 1929
BsaHI GRCGYC 1 cut(s) 3300
BsaI GGTCTC 2 cut(s) 1994, 2462
BsaJI CCNNGG 5 cut(s) 566, 863, 1036, 1201, 1315
Bsc4I CCNNNNNNNGG 8 cut(s) 623, 1042, 1479, 1737, 1807, 2096, 2453, 2935
Bse3DI GCAATG 5 cut(s) 2076, 2172, 2395, 3090, 3132
Bse8I GATNNNNATC 2 cut(s) 195, 1929
BseBI CCWGG 2 cut(s) 1202, 2877
BseCI ATCGAT 1 cut(s) 1226
BseDI CCNNGG 5 cut(s) 566, 863, 1036, 1201, 1315
BseJI GATNNNNATC 2 cut(s) 195, 1929
BseLI CCNNNNNNNGG 8 cut(s) 623, 1042, 1479, 1737, 1807, 2096, 2453, 2935
BseMI GCAATG 5 cut(s) 2076, 2172, 2395, 3090, 3132
BseMII CTCAG 7 cut(s) 362, 426, 1335, 2005, 2267, 2825, 3136
BseRI GAGGAG 2 cut(s) 68, 341
BseSI GKGCMC 6 cut(s) 637, 871, 1485, 1732, 3006, 3178
BseX3I CGGCCG 1 cut(s) 3341
BseXI GCAGC 7 cut(s) 339, 556, 649, 799, 844, 2455, 3317
BsgI GTGCAG 1 cut(s) 927
Bsh1236I CGCG 3 cut(s) 2017, 2777, 3071
Bsh1285I CGRYCG 1 cut(s) 3344
BshFI GGCC 3 cut(s) 571, 2137, 3343
BshNI GGYRCC 5 cut(s) 868, 1489, 1727, 2801, 3001
BshVI ATCGAT 1 cut(s) 1226
BsiEI CGRYCG 1 cut(s) 3344
BsiHKAI GWGCWC 4 cut(s) 631, 637, 2232, 3178
BsiHKCI CYCGRG 5 cut(s) 864, 1340, 1596, 2480, 2683
BsiSI CCGG 2 cut(s) 734, 1616
BslFI GGGAC 3 cut(s) 1632, 1690, 3340
BslI CCNNNNNNNGG 8 cut(s) 623, 1042, 1479, 1737, 1807, 2096, 2453, 2935
BsmAI GTCTC 6 cut(s) 1686, 1994, 2462, 2797, 2942, 3112
BsmFI GGGAC 3 cut(s) 1632, 1690, 3340
BsmI GAATGC 3 cut(s) 2765, 2988, 2990
BsnI GGCC 3 cut(s) 571, 2137, 3343
Bso31I GGTCTC 2 cut(s) 1994, 2462
BsoBI CYCGRG 5 cut(s) 864, 1340, 1596, 2480, 2683
Bsp119I TTCGAA 1 cut(s) 2975
Bsp1286I GDGCHC 9 cut(s) 631, 637, 871, 1256, 1485, 1732, 2232, 3006, 3178
Bsp1407I TGTACA 1 cut(s) 1628
Bsp1720I GCTNAGC 1 cut(s) 2811
BspANI GGCC 3 cut(s) 571, 2137, 3343
BspCNI CTCAG 7 cut(s) 361, 427, 1334, 2004, 2266, 2824, 3135
BspDI ATCGAT 1 cut(s) 1226
BspFNI CGCG 3 cut(s) 2017, 2777, 3071
BspHI TCATGA 1 cut(s) 924
BspOI GCTAGC 1 cut(s) 485
BspQI GCTCTTC 2 cut(s) 1472, 2822
BspT104I TTCGAA 1 cut(s) 2975
BspT107I GGYRCC 5 cut(s) 868, 1489, 1727, 2801, 3001
BspTI CTTAAG 1 cut(s) 1999
BspTNI GGTCTC 2 cut(s) 1994, 2462
BsrBI CCGCTC 1 cut(s) 1550
BsrDI GCAATG 5 cut(s) 2076, 2172, 2395, 3090, 3132
BsrGI TGTACA 1 cut(s) 1628
BssECI CCNNGG 5 cut(s) 566, 863, 1036, 1201, 1315
BssNAI GTATAC 2 cut(s) 432, 2690
BssNI GRCGYC 1 cut(s) 3300
BssSI CACGAG 6 cut(s) 630, 914, 1239, 1484, 2836, 2901
BssT1I CCWWGG 3 cut(s) 566, 1036, 1315
Bst1107I GTATAC 2 cut(s) 432, 2690
Bst2BI CACGAG 6 cut(s) 630, 914, 1239, 1484, 2836, 2901
Bst2UI CCWGG 2 cut(s) 1202, 2877
Bst6I CTCTTC 5 cut(s) 120, 1472, 2800, 2822, 3025
BstACI GRCGYC 1 cut(s) 3300
BstAFI CTTAAG 1 cut(s) 1999
BstAUI TGTACA 1 cut(s) 1628
BstBAI YACGTR 1 cut(s) 2536
BstBI TTCGAA 1 cut(s) 2975
BstC8I GCNNGC 5 cut(s) 483, 512, 1089, 2122, 2958
BstDEI CTNAG 8 cut(s) 46, 348, 435, 1321, 1991, 2253, 2811, 3122
BstEII GGTNACC 1 cut(s) 2738
BstFNI CGCG 3 cut(s) 2017, 2777, 3071
BstHHI GCGC 2 cut(s) 2017, 2779
BstMAI GTCTC 6 cut(s) 1686, 1994, 2462, 2797, 2942, 3112
BstMCI CGRYCG 1 cut(s) 3344
BstMWI GCNNNNNNNGC 8 cut(s) 550, 668, 743, 2012, 2036, 2443, 3077, 3143
BstNI CCWGG 2 cut(s) 1202, 2877
BstNSI RCATGY 1 cut(s) 3154
BstPAI GACNNNNGTC 2 cut(s) 1329, 3343
BstPI GGTNACC 1 cut(s) 2738
BstSCI CCNGG 3 cut(s) 1200, 1615, 2875
BstSFI CTRYAG 3 cut(s) 1209, 1642, 1947
BstSLI GKGCMC 6 cut(s) 637, 871, 1485, 1732, 3006, 3178
BstSNI TACGTA 1 cut(s) 2536
BstUI CGCG 3 cut(s) 2017, 2777, 3071
BstV1I GCAGC 7 cut(s) 339, 556, 649, 799, 844, 2455, 3317
BstV2I GAAGAC 1 cut(s) 1827
BstX2I RGATCY 3 cut(s) 1133, 1968, 2382
BstXI CCANNNNNNTGG 1 cut(s) 2439
BstYI RGATCY 3 cut(s) 1133, 1968, 2382
BstZ17I GTATAC 2 cut(s) 432, 2690
BstZI CGGCCG 1 cut(s) 3341
Bsu15I ATCGAT 1 cut(s) 1226
BsuI GTATCC 1 cut(s) 2068
BsuRI GGCC 3 cut(s) 571, 2137, 3343
BsuTUI ATCGAT 1 cut(s) 1226
BtsIMutI CAGTG 4 cut(s) 1628, 1651, 2261, 3178
Cac8I GCNNGC 5 cut(s) 483, 512, 1089, 2122, 2958
CaiI CAGNNNCTG 2 cut(s) 662, 3329
CciI TCATGA 1 cut(s) 924
CfoI GCGC 2 cut(s) 2017, 2779
Cfr13I GGNCC 2 cut(s) 710, 2826
ClaI ATCGAT 1 cut(s) 1226
CseI GACGC 5 cut(s) 981, 1619, 2126, 3060, 3289
Csp6I GTAC 3 cut(s) 118, 1026, 1629
CviQI GTAC 3 cut(s) 118, 1026, 1629
DdeI CTNAG 8 cut(s) 46, 348, 435, 1321, 1991, 2253, 2811, 3122
DraIII CACNNNGTG 1 cut(s) 1122
DrdI GACNNNNNNGTC 1 cut(s) 2903
DseDI GACNNNNNNGTC 1 cut(s) 2903
EaeI YGGCCR 1 cut(s) 3341
EagI CGGCCG 1 cut(s) 3341
Eam1104I CTCTTC 5 cut(s) 120, 1472, 2800, 2822, 3025
EarI CTCTTC 5 cut(s) 120, 1472, 2800, 2822, 3025
Ecl136II GAGCTC 1 cut(s) 2230
EclXI CGGCCG 1 cut(s) 3341
Eco105I TACGTA 1 cut(s) 2536
Eco130I CCWWGG 3 cut(s) 566, 1036, 1315
Eco147I AGGCCT 1 cut(s) 571
Eco24I GRGCYC 2 cut(s) 1256, 2232
Eco31I GGTCTC 2 cut(s) 1994, 2462
Eco32I GATATC 1 cut(s) 1230
Eco47I GGWCC 2 cut(s) 710, 2826
Eco52I CGGCCG 1 cut(s) 3341
Eco53kI GAGCTC 1 cut(s) 2230
Eco57I CTGAAG 3 cut(s) 1902, 2511, 2841
Eco88I CYCGRG 5 cut(s) 864, 1340, 1596, 2480, 2683
Eco91I GGTNACC 1 cut(s) 2738
EcoICRI GAGCTC 1 cut(s) 2230
EcoO65I GGTNACC 1 cut(s) 2738
EcoRII CCWGG 2 cut(s) 1200, 2875
EcoRV GATATC 1 cut(s) 1230
EcoT14I CCWWGG 3 cut(s) 566, 1036, 1315
EcoT22I ATGCAT 1 cut(s) 2990
EcoT38I GRGCYC 2 cut(s) 1256, 2232
ErhI CCWWGG 3 cut(s) 566, 1036, 1315
FalI AAGNNNNNCTT 6 cut(s) 470, 502, 1451, 1483, 1875, 1907
FaqI GGGAC 3 cut(s) 1632, 1690, 3340
FauI CCCGC 1 cut(s) 2098
FbaI TGATCA 3 cut(s) 522, 910, 3195
FblI GTMKAC 2 cut(s) 431, 2689
FriOI GRGCYC 2 cut(s) 1256, 2232
FspBI CTAG 9 cut(s) 83, 254, 482, 884, 1011, 2421, 2762, 3282, 3348
GlaI GCGC 2 cut(s) 2016, 2778
GsuI CTGGAG 4 cut(s) 23, 162, 383, 1791
HaeIII GGCC 3 cut(s) 571, 2137, 3343
HapII CCGG 2 cut(s) 734, 1616
HgaI GACGC 5 cut(s) 981, 1619, 2126, 3060, 3289
HhaI GCGC 2 cut(s) 2017, 2779
Hin1I GRCGYC 1 cut(s) 3300
Hin6I GCGC 2 cut(s) 2015, 2777
HinP1I GCGC 2 cut(s) 2015, 2777
HincII GTYRAC 1 cut(s) 1334
HindII GTYRAC 1 cut(s) 1334
HindIII AAGCTT 2 cut(s) 501, 532
HpaII CCGG 2 cut(s) 734, 1616
Hpy99I CGWCG 2 cut(s) 1709, 3305
HpyCH4IV ACGT 3 cut(s) 1330, 2535, 2703
HpyF10VI GCNNNNNNNGC 8 cut(s) 550, 668, 743, 2012, 2036, 2443, 3077, 3143
HpyF3I CTNAG 8 cut(s) 46, 348, 435, 1321, 1991, 2253, 2811, 3122
HpySE526I ACGT 3 cut(s) 1330, 2535, 2703
Hsp92I GRCGYC 1 cut(s) 3300
HspAI GCGC 2 cut(s) 2015, 2777
Ksp22I TGATCA 3 cut(s) 522, 910, 3195
LguI GCTCTTC 2 cut(s) 1472, 2822
LmnI GCTCC 4 cut(s) 328, 736, 742, 1251
Lsp1109I GCAGC 7 cut(s) 339, 556, 649, 799, 844, 2455, 3317
MaeI CTAG 9 cut(s) 83, 254, 482, 884, 1011, 2421, 2762, 3282, 3348
MaeII ACGT 3 cut(s) 1330, 2535, 2703
MbiI CCGCTC 1 cut(s) 1550
MflI RGATCY 3 cut(s) 1133, 1968, 2382
MhlI GDGCHC 9 cut(s) 631, 637, 871, 1256, 1485, 1732, 2232, 3006, 3178
MluI ACGCGT 1 cut(s) 3069
MlyI GAGTC 9 cut(s) 1688, 2195, 2245, 2282, 2522, 2675, 2828, 2913, 3114
MmeI TCCRAC 5 cut(s) 978, 1111, 2273, 2426, 3214
Mph1103I ATGCAT 1 cut(s) 2990
MroXI GAANNNNTTC 7 cut(s) 72, 215, 308, 1466, 1527, 2560, 2988
MslI CAYNNNNRTG 2 cut(s) 1305, 2048
MspA1I CMGCKG 6 cut(s) 352, 1769, 2454, 2831, 3308, 3359
MspCI CTTAAG 1 cut(s) 1999
MspI CCGG 2 cut(s) 734, 1616
MspR9I CCNGG 3 cut(s) 1202, 1617, 2877
Mva1269I GAATGC 3 cut(s) 2765, 2988, 2990
MvaI CCWGG 2 cut(s) 1202, 2877
MvnI CGCG 3 cut(s) 2017, 2777, 3071
MwoI GCNNNNNNNGC 8 cut(s) 550, 668, 743, 2012, 2036, 2443, 3077, 3143
NciI CCSGG 1 cut(s) 1617
NheI GCTAGC 1 cut(s) 481
NmeAIII GCCGAG 1 cut(s) 2287
NmuCI GTSAC 8 cut(s) 961, 993, 1114, 1150, 1234, 1411, 2266, 2738
NsiI ATGCAT 1 cut(s) 2990
NspI RCATGY 1 cut(s) 3154
NspV TTCGAA 1 cut(s) 2975
PaeR7I CTCGAG 2 cut(s) 1340, 2683
PagI TCATGA 1 cut(s) 924
PceI AGGCCT 1 cut(s) 571
PciSI GCTCTTC 2 cut(s) 1472, 2822
PcsI WCGNNNNNNNCGW 1 cut(s) 2964
PctI GAATGC 3 cut(s) 2765, 2988, 2990
PdmI GAANNNNTTC 7 cut(s) 72, 215, 308, 1466, 1527, 2560, 2988
PflMI CCANNNNNTGG 1 cut(s) 2935
PleI GAGTC 9 cut(s) 1688, 2195, 2245, 2282, 2522, 2675, 2828, 2912, 3114
PpsI GAGTC 9 cut(s) 1688, 2195, 2245, 2282, 2522, 2675, 2828, 2912, 3114
Ppu21I YACGTR 1 cut(s) 2536
PshAI GACNNNNGTC 2 cut(s) 1329, 3343
PshBI ATTAAT 1 cut(s) 890
Psp124BI GAGCTC 1 cut(s) 2232
Psp6I CCWGG 2 cut(s) 1200, 2875
PspEI GGTNACC 1 cut(s) 2738
PspGI CCWGG 2 cut(s) 1200, 2875
PspPI GGNCC 2 cut(s) 710, 2826
PspXI VCTCGAGB 1 cut(s) 2683
PstNI CAGNNNCTG 2 cut(s) 662, 3329
PsuI RGATCY 3 cut(s) 1133, 1968, 2382
PvuII CAGCTG 3 cut(s) 352, 1769, 3308
RsaI GTAC 3 cut(s) 119, 1027, 1630
RsaNI GTAC 3 cut(s) 118, 1026, 1629
RseI CAYNNNNRTG 2 cut(s) 1305, 2048
SacI GAGCTC 1 cut(s) 2232
SapI GCTCTTC 2 cut(s) 1472, 2822
Sau96I GGNCC 2 cut(s) 710, 2826
ScaI AGTACT 1 cut(s) 119
SchI GAGTC 9 cut(s) 1688, 2195, 2245, 2282, 2522, 2675, 2828, 2913, 3114
ScrFI CCNGG 3 cut(s) 1202, 1617, 2877
SduI GDGCHC 9 cut(s) 631, 637, 871, 1256, 1485, 1732, 2232, 3006, 3178
SfcI CTRYAG 3 cut(s) 1209, 1642, 1947
Sfr274I CTCGAG 2 cut(s) 1340, 2683
SfuI TTCGAA 1 cut(s) 2975
SinI GGWCC 2 cut(s) 710, 2826
SlaI CTCGAG 2 cut(s) 1340, 2683
SmiMI CAYNNNNRTG 2 cut(s) 1305, 2048
SmlI CTYRAG 6 cut(s) 1340, 1999, 2646, 2683, 3115, 3370
SmoI CTYRAG 6 cut(s) 1340, 1999, 2646, 2683, 3115, 3370
SnaBI TACGTA 1 cut(s) 2536
SseBI AGGCCT 1 cut(s) 571
SspI AATATT 3 cut(s) 1528, 2659, 2785
SspMI CTAG 9 cut(s) 83, 254, 482, 884, 1011, 2421, 2762, 3282, 3348
SstI GAGCTC 1 cut(s) 2232
StuI AGGCCT 1 cut(s) 571
StyD4I CCNGG 3 cut(s) 1200, 1615, 2875
StyI CCWWGG 3 cut(s) 566, 1036, 1315
TaiI ACGT 3 cut(s) 1333, 2538, 2706
TatI WGTACW 3 cut(s) 117, 1025, 1628
TauI GCSGC 4 cut(s) 1110, 1184, 3132, 3362
TscAI CASTG 4 cut(s) 1628, 1651, 2261, 3178
TseFI GTSAC 8 cut(s) 961, 993, 1114, 1150, 1234, 1411, 2266, 2738
TseI GCWGC 7 cut(s) 352, 544, 662, 812, 832, 2443, 3305
Tsp45I GTSAC 8 cut(s) 961, 993, 1114, 1150, 1234, 1411, 2266, 2738
TspDTI ATGAA 9 cut(s) 168, 224, 777, 941, 2066, 2593, 2630, 2981, 3342
TspGWI ACGGA 2 cut(s) 1222, 2493
TspRI CASTG 4 cut(s) 1628, 1651, 2261, 3178
Van91I CCANNNNNTGG 1 cut(s) 2935
Vha464I CTTAAG 1 cut(s) 1999
VneI GTGCAC 2 cut(s) 633, 3174
VpaK11BI GGWCC 2 cut(s) 710, 2826
VspI ATTAAT 1 cut(s) 890
XapI RAATTY 3 cut(s) 367, 1835, 2323
XbaI TCTAGA 2 cut(s) 253, 3281
XceI RCATGY 1 cut(s) 3154
XcmI CCANNNNNNNNNTGG 1 cut(s) 2068
XhoI CTCGAG 2 cut(s) 1340, 2683
XmiI GTMKAC 2 cut(s) 431, 2689
XmnI GAANNNNTTC 7 cut(s) 72, 215, 308, 1466, 1527, 2560, 2988
XspI CTAG 9 cut(s) 83, 254, 482, 884, 1011, 2421, 2762, 3282, 3348
ZrmI AGTACT 1 cut(s) 119
Zsp2I ATGCAT 1 cut(s) 2990
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.