Rh4AG250300

Topless-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0013792
Physical Location & Seq
Forward (+)
56231985 .. 56239759
7775 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG250300.1

Sequence Viewer

Length: 2916 bp
ATGTCATCTCTGAGCAGAGAGCTGGTTTTCCTGATCCTTCAATTTCTGGAGGAGGAGAAATTCAAGGAGGCTGTACATAGGCTCGAGCAAGAGTCTGGATTCTTCTTCAACATGAAGTACTTCGAGGAGAAAGCGTTGCAGGGAGAGTGGGACGAGGTGGAGAAGTACCTGTCCGGGTTCACCAAGGTGGACGAGAATCGCTACTCTATGAAGATCTTCTTCGAGGTCAGAAAGCAGAAGTATCTGGAAGCGCTGGACAGGCAAGACAGGGCGAAAGCTGTTGAGATACTGGTGAAGGATTTGAAGGTGTTTTCGACGTTTAATGAGGACTTGTACAAGGAAATTACTCATCTGTTGACTCTTGACAACTTCAGAGAAAATGAGCAGTTGTCCAAGTATGGTGATACTAAATCCGCTAGAAGCATAATGCTGGTAGAGCTGAAGAAATTGATAGAAGCAAATCCTCTATTCCGAGAGAAGCTGGTTTTCCCCACTTTGAAGGCGTCACGCTTGCGAACTTTAATCAATCAAAGTTTAAATTGGCAGCACCAGCTATGCAAAAACCCAAGGCCAAATCCTGACATTAAGACCTTATTCATGGACCACTCGTGTTCTCCTCCAAATGGAGCTCGTGCATCGACTCCAGTTACTCTTCCAGTTGCAGCTCTTACAAAGCCTTTAACTTATGCTCCTCTTGGAGCACATGGTGGACCTTTCCCGCCGGCTGCTGCTGCTGCAGCTGCCAATGCGAATGCTTTAGCAGGATGGATGTCCAATGCTAATCCGTCCTTGTCTATGCAATCAGCTCCGGTCTCTGCTTCACCCCTGCCTGTTCAACTTGGTCAAGTTTCTTCTTTAAAGCATCCAAGAACACCATCAACTGGTCTTGGAATGATTGATTATCAAGGCTCTGATCATGAGCAACTAATGAAACGCCTGCGGACTGCACAATCTGTTGATGAGGTTAGCTATCCTCCTCAACATGCTTCCTGGTCGCTAGATGACCTACCGAGAAATGTATCTTGTACACTTCGTCAGGGATCCACTGTTATGAGCATGGATTTCCACCCTTCTCATCACACATTACTTGCTGTTGGCTGTAGTAATGGAGAAGTTACTCTGTGGGAAGTTGGGTTACGAGAGAAGCTGGTGTCCAAGCCATTCAAAGTATGGGAAATGACTACTTGTTCTCTGACATTTCAGGCGGCTATTGTCAAAGATTCATCAATGTCTGTTAACCGTGTATCATGGAGTCCAGATGGGAACTTAATCGGTGTTGCATTTACGAAACATTTGGTTCATCTGTATGCTTATCAAGGGTCTAATGATCTACGCCAACATTTGGAGATTGATGCCCACAGTGGAGGTGTGAATGACTTGGCATTCTCTCATCCAAACAAGCAATTGTGCGTTGTAACATGTGGAGATGATAAGATGATAAAGGTTTGGGATCTGGCTGGAAGGAAGCTTTTCCACTTTGAAGGTCATGAAGCACCTGTTTACTCGGTTTGCCCCCACCAGAAGGAGAATATTCAGTTTATATTTTCAACTGCTGTTGATGGAAAAATAAAAGCTTGGCTGTATGACAATGTGGGCTCCAGGGTTGACTATGATGCTCCAGGGCACTGGTGCACCACAATGCTGTACAGTGATGATGGAAATAGATTGTTCTCTTGTGGGACTAGTAAAGATGGTGACTCCTTCCTGGTTGAATGGAATGAAAGTGAAGGGGCAATAAAGAGGACCTATAGTGGGTTCAGAAAGAAATCTAATGGCATCGCTCAATTTGATACCACAAGAAATCACTTTTTGGCTGTTGGTGAAGATAGCCAGATAAAATTTTGGGATATGGATAATACAAATGTTCTTCTCAGTATAGAAGCTGATGGTGGACTACCACCTGTTCCTCGCTTGAGATTCAATAAGGAAGGGAATCTGCTTGTTGTTACCACGGCTGACAATGGATTCAAGGTTCTTGCAAATGCTGATGGTCTTAGATCCTTAAGAGCAATTGAAAATCGATCTTATGAAGCATCAAGAACACCTATTGAGATGAAGGTATCTACTTCGGCCATGGTTCCAAATATCAATCCTGCCATTAATAAAGTAGATCGTATGGATGCAAGCTCTCCTGCCAGACCTACTCAGATTCTTAATGGAGTTGATCCTATGGCAAGAAGTGTAGAAAAGCGAAGACTATTAGATGATGCATCCGAGAAAAATAAACGTTGGGAACTGGCAGAGATTGTTGATCCTGTTCACTGTCGAGTGGCTACGATGCCAGATAGTAAAGATCCTGCTAACAAGGTTGCTCGACTTCTGTACACAAATTCTGGTGCCGGCATTCTGGCTCTTGGTTCAAATGGGGTCCAGAAGCTATGGAAGTGGAGAAGCAGTGAACAAAATCCAAATGGAAAGGCCACTGCTAGTGTCGTTCCGCAACATTGGCAACCCAACAATGGTCTTTTGATGACTAATGATGTCCCCGAAAACTGTGAGGAAGCAGTTCCATGTATTGCACTCTCAAAGAATGACTCCTATGTGATGTCGGCATGTGGCGGAAAAGTGTCTCTGTTCAATATGATGACATTTAAGGTAATGACAACTTTCATGTCACCTCCACCAGCCTCAACCTTCCTAGCATTTCATCCTCTGGATAATAACATCATAGCAATTGGAACAGAAGATTCAACTATCCACATTTACAATGTTAGAGTTGATGAGGTCAAATCAAAACTGAAAGGCCACCAGAAACACATTACTGGATTAGCGTTTTCTGTTAACCTTAAGATCATGGTTTCATCAGGTGCTGATGCTCAAGTACGTGACATCCATTCTTTCTTATGTTATTGGAACAACTTGTCAACTTTTCCTGTTAATGTGTGTTTTGGTCTCTGTGTCATTGTAATTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

971

Amino Acids

108.21

Weight (kDa)

7.15

Isoelectric Point (pI)

41.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH_TPL PF17814 5 - 34 3e-11 LisH-like dimerisation domain
TPR1-like_2nd PF21889 37 - 161 7.7e-62 TPR1-like, CTLH-containing domain
zf_topless PF21359 164 - 204 2.4e-25 TOPLESS, zinc finger domain
Beta-prop_EML_2 PF23414 331 - 467 5.2e-08 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd PF25172 348 - 563 4.5e-11 WDR3 second beta-propeller domain
Beta-prop_RIG_2nd PF23775 358 - 499 5.4e-07 RIG second beta-propeller
Beta-prop_WDR3_1st PF25173 389 - 526 4.9e-14 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 389 - 529 2.7e-06 WDR36/Utp21 first beta-propeller
WD40_Prp19 PF24814 392 - 491 7.1e-09 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 403 - 560 3.3e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 407 - 622 2.7e-21 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 410 - 663 1.2e-20 THOC3 beta-propeller domain
WDR55 PF24796 411 - 526 4.2e-08 WDR55
Beta-prop_EML PF23409 448 - 652 6.8e-08 Echinoderm microtubule-associated protein first beta-propeller
WD40 PF00400 449 - 484 1.5e-06 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 457 - 529 3.1e-08 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 464 - 566 2.2e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 466 - 658 3.5e-10 Prp19 WD40 domain
WDR55 PF24796 512 - 657 2.5e-06 WDR55
Beta-prop_WDR3_1st PF25173 519 - 658 2.9e-07 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 816 - 942 3.8e-13 Prp19 WD40 domain
WDR55 PF24796 830 - 942 3.7e-06 WDR55
Beta-prop_WDR3_1st PF25173 830 - 942 9e-13 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 831 - 942 7.3e-10 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 831 - 939 1.2e-08 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 832 - 942 1.2e-10 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 832 - 942 5.3e-08 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 833 - 942 2.7e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 836 - 942 6.6e-10 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 841 - 942 7e-07 THOC3 beta-propeller domain
Beta-prop_IFT140_1st PF23383 849 - 936 7.8e-06 IFT140 first beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 853 - 939 9.5e-08 WDR36/Utp21 first beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 855 - 938 3.2e-07 WDR90/POC16, second beta-propeller
Beta-prop_RIG_1st PF23770 863 - 926 4.4e-06 RIG first beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013792)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G16830
fragaria_vesca FvH4_4g18910 FvH4_4g18910
malus_domestica MD13G1162100.v1.1 MD16G1161900.v1.1
prunus_persica Prupe.1G129400_v2.0.a1
pyrus_communis pycom13g13830
rosa_chinensis RchiOBHm_Chr4g0423811
rosa_laevigata RLG00000007530
rosa_multiflora Rmu_sc0003369.1_g000018
rosa_roxburghii Rroxscaffold_5G00365920
rosa_rugosa Rorug04G0190900
rosa_samantha Rh4AG250300 Rh4BG255600 Rh4CG263700 Rh4DG250000
rosa_wichuraiana Rw4G021430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 2336
AccB7I CCANNNNNTGG 2 cut(s) 881, 1342
AciI CCGC 6 cut(s) 414, 719, 940, 1205, 2438, 2559
AclI AACGTT 1 cut(s) 2227
AclWI GGATC 8 cut(s) 28, 1035, 1048, 1458, 1992, 2159, 2246, 2288
AcoI YGGCCR 1 cut(s) 2070
AcsI RAATTY 3 cut(s) 59, 1838, 2329
AcuI CTGAAG 2 cut(s) 355, 461
AcyI GRCGYC 1 cut(s) 503
AdeI CACNNNGTG 1 cut(s) 707
AfaI GTAC 8 cut(s) 75, 119, 167, 335, 1027, 1646, 2324, 2823
AfeI AGCGCT 1 cut(s) 252
AfiI CCNNNNNNNGG 6 cut(s) 623, 881, 1342, 1522, 1752, 2459
AflII CTTAAG 2 cut(s) 2002, 2786
AflIII ACRYGT 1 cut(s) 1418
AhlI ACTAGT 1 cut(s) 1682
AjnI CCWGG 4 cut(s) 989, 1598, 1618, 1704
AleI CACNNNNGTG 1 cut(s) 185
AloI GAACNNNNNNTCC 2 cut(s) 1956, 1988
Alw21I GWGCWC 3 cut(s) 631, 703, 1634
Alw26I GTCTC 3 cut(s) 817, 2574, 2897
Alw44I GTGCAC 1 cut(s) 1630
AlwI GGATC 8 cut(s) 28, 1035, 1048, 1458, 1992, 2159, 2246, 2288
AlwNI CAGNNNCTG 1 cut(s) 2810
Ama87I CYCGRG 1 cut(s) 83
Aor51HI AGCGCT 1 cut(s) 252
AoxI GGCC 4 cut(s) 569, 2070, 2418, 2743
ApaLI GTGCAC 1 cut(s) 1630
ApeKI GCWGC 8 cut(s) 544, 662, 725, 728, 731, 734, 737, 740
ApoI RAATTY 3 cut(s) 59, 1838, 2329
AseI ATTAAT 1 cut(s) 2100
Asp700I GAANNNNTTC 4 cut(s) 119, 215, 1469, 2505
AspLEI GCGC 1 cut(s) 253
AspS9I GGNCC 4 cut(s) 601, 710, 1743, 2368
AsuC2I CCSGG 1 cut(s) 175
AsuHPI GGTGA 7 cut(s) 172, 304, 413, 813, 1706, 1832, 2607
AvaI CYCGRG 1 cut(s) 83
AvaII GGWCC 4 cut(s) 601, 710, 1743, 2368
BaeGI GKGCMC 2 cut(s) 1626, 1634
BamHI GGATCC 1 cut(s) 1040
BanI GGYRCC 1 cut(s) 2336
BanII GRGCYC 2 cut(s) 631, 1598
BarI GAAGNNNNNNTAC 2 cut(s) 1119, 1151
BauI CACGAG 2 cut(s) 607, 630
BbsI GAAGAC 1 cut(s) 2200
Bbv12I GWGCWC 3 cut(s) 631, 703, 1634
BbvI GCAGC 8 cut(s) 556, 674, 712, 715, 718, 721, 727, 749
BccI CCATC 8 cut(s) 759, 883, 1253, 1553, 1649, 1685, 1880, 1982
BceAI ACGGC 1 cut(s) 1968
BciT130I CCWGG 4 cut(s) 991, 1600, 1620, 1706
BclI TGATCA 1 cut(s) 913
BcnI CCSGG 1 cut(s) 175
BcoDI GTCTC 3 cut(s) 817, 2574, 2897
BcuI ACTAGT 1 cut(s) 1682
BfaI CTAG 5 cut(s) 417, 998, 1683, 2427, 2639
BfmI CTRYAG 3 cut(s) 735, 1099, 1747
BfoI RGCGCY 1 cut(s) 254
BfrI CTTAAG 2 cut(s) 2002, 2786
BglII AGATCT 1 cut(s) 213
BisI GCNGC 9 cut(s) 545, 663, 726, 729, 732, 735, 738, 741, 1206
BlsI GCNGC 9 cut(s) 546, 664, 727, 730, 733, 736, 739, 742, 1207
BmcAI AGTACT 1 cut(s) 119
Bme1390I CCNGG 5 cut(s) 175, 991, 1600, 1620, 1706
Bme18I GGWCC 4 cut(s) 601, 710, 1743, 2368
BmeT110I CYCGRG 1 cut(s) 83
BmgT120I GGNCC 4 cut(s) 601, 710, 1743, 2368
BmiI GGNNCC 5 cut(s) 1042, 1597, 2079, 2338, 2369
BmrFI CCNGG 5 cut(s) 175, 991, 1600, 1620, 1706
BpiI GAAGAC 1 cut(s) 2200
BpmI CTGGAG 4 cut(s) 68, 627, 1582, 1602
BpuEI CTTGAG 2 cut(s) 1933, 2802
BpuMI CCSGG 1 cut(s) 175
Bsa29I ATCGAT 1 cut(s) 2020
BsaAI YACGTR 1 cut(s) 2825
BsaHI GRCGYC 1 cut(s) 503
BsaI GGTCTC 2 cut(s) 817, 2897
BsaJI CCNNGG 6 cut(s) 183, 566, 1599, 1619, 1950, 2073
BsaWI WCCGGW 1 cut(s) 808
BsaXI ACNNNNNCTCC 6 cut(s) 119, 149, 673, 703, 2380, 2410
Bsc4I CCNNNNNNNGG 6 cut(s) 623, 881, 1342, 1522, 1752, 2459
Bse118I RCCGGY 2 cut(s) 721, 2339
Bse1I ACTGG 7 cut(s) 294, 644, 656, 886, 1631, 2241, 2767
BseBI CCWGG 4 cut(s) 991, 1600, 1620, 1706
BseCI ATCGAT 1 cut(s) 2020
BseDI CCNNGG 6 cut(s) 183, 566, 1599, 1619, 1950, 2073
BseGI GGATG 8 cut(s) 770, 774, 862, 1390, 2125, 2210, 2647, 2829
BseLI CCNNNNNNNGG 6 cut(s) 623, 881, 1342, 1522, 1752, 2459
BseMII CTCAG 2 cut(s) 1885, 2159
BseNI ACTGG 7 cut(s) 294, 644, 656, 886, 1631, 2241, 2767
BseRI GAGGAG 6 cut(s) 65, 68, 140, 606, 681, 966
BseSI GKGCMC 2 cut(s) 1626, 1634
BseXI GCAGC 8 cut(s) 556, 674, 712, 715, 718, 721, 727, 749
BsgI GTGCAG 1 cut(s) 930
BshFI GGCC 4 cut(s) 571, 2072, 2420, 2745
BshNI GGYRCC 1 cut(s) 2336
BshVI ATCGAT 1 cut(s) 2020
BsiHKAI GWGCWC 3 cut(s) 631, 703, 1634
BsiHKCI CYCGRG 1 cut(s) 83
BsiSI CCGG 4 cut(s) 174, 722, 809, 2340
BslFI GGGAC 3 cut(s) 164, 1693, 2468
BslI CCNNNNNNNGG 6 cut(s) 623, 881, 1342, 1522, 1752, 2459
BsmAI GTCTC 3 cut(s) 817, 2574, 2897
BsmFI GGGAC 3 cut(s) 164, 1693, 2468
BsmI GAATGC 3 cut(s) 757, 1382, 2343
BsnI GGCC 4 cut(s) 571, 2072, 2420, 2745
Bso31I GGTCTC 2 cut(s) 817, 2897
BsoBI CYCGRG 1 cut(s) 83
Bsp1286I GDGCHC 5 cut(s) 631, 703, 1598, 1626, 1634
Bsp1407I TGTACA 5 cut(s) 73, 333, 1025, 1644, 2322
Bsp19I CCATGG 1 cut(s) 2073
BspACI CCGC 6 cut(s) 414, 719, 940, 1205, 2438, 2559
BspANI GGCC 4 cut(s) 571, 2072, 2420, 2745
BspCNI CTCAG 2 cut(s) 1884, 2158
BspDI ATCGAT 1 cut(s) 2020
BspHI TCATGA 2 cut(s) 916, 1486
BspLI GGNNCC 5 cut(s) 1042, 1597, 2079, 2338, 2369
BspMAI CTGCAG 1 cut(s) 739
BspPI GGATC 8 cut(s) 28, 1035, 1048, 1458, 1992, 2159, 2246, 2288
BspT107I GGYRCC 1 cut(s) 2336
BspTI CTTAAG 2 cut(s) 2002, 2786
BspTNI GGTCTC 2 cut(s) 817, 2897
BsrFI RCCGGY 2 cut(s) 721, 2339
BsrGI TGTACA 5 cut(s) 73, 333, 1025, 1644, 2322
BsrI ACTGG 7 cut(s) 294, 644, 656, 886, 1631, 2241, 2767
BssAI RCCGGY 2 cut(s) 721, 2339
BssECI CCNNGG 6 cut(s) 183, 566, 1599, 1619, 1950, 2073
BssNI GRCGYC 1 cut(s) 503
BssSI CACGAG 2 cut(s) 607, 630
BssT1I CCWWGG 3 cut(s) 183, 566, 2073
Bst2BI CACGAG 2 cut(s) 607, 630
Bst2UI CCWGG 4 cut(s) 991, 1600, 1620, 1706
Bst4CI ACNGT 6 cut(s) 1048, 1241, 1361, 1649, 2264, 2495
Bst6I CTCTTC 1 cut(s) 657
BstACI GRCGYC 1 cut(s) 503
BstAFI CTTAAG 2 cut(s) 2002, 2786
BstAUI TGTACA 5 cut(s) 73, 333, 1025, 1644, 2322
BstBAI YACGTR 1 cut(s) 2825
BstC8I GCNNGC 5 cut(s) 512, 723, 938, 2125, 2341
BstDEI CTNAG 4 cut(s) 11, 1871, 1994, 2145
BstDSI CCRYGG 2 cut(s) 1950, 2073
BstF5I GGATG 8 cut(s) 770, 774, 862, 1390, 2125, 2210, 2647, 2829
BstH2I RGCGCY 1 cut(s) 254
BstHHI GCGC 1 cut(s) 253
BstMAI GTCTC 3 cut(s) 817, 2574, 2897
BstMWI GCNNNNNNNGC 9 cut(s) 259, 436, 550, 731, 734, 737, 740, 746, 2446
BstNI CCWGG 4 cut(s) 991, 1600, 1620, 1706
BstNSI RCATGY 3 cut(s) 986, 1422, 2556
BstSCI CCNGG 5 cut(s) 173, 989, 1598, 1618, 1704
BstSFI CTRYAG 3 cut(s) 735, 1099, 1747
BstSLI GKGCMC 2 cut(s) 1626, 1634
BstV1I GCAGC 8 cut(s) 556, 674, 712, 715, 718, 721, 727, 749
BstV2I GAAGAC 1 cut(s) 2200
BstX2I RGATCY 5 cut(s) 213, 1040, 1450, 1997, 2293
BstXI CCANNNNNNTGG 1 cut(s) 1626
BstYI RGATCY 5 cut(s) 213, 1040, 1450, 1997, 2293
Bsu15I ATCGAT 1 cut(s) 2020
BsuRI GGCC 4 cut(s) 571, 2072, 2420, 2745
BsuTUI ATCGAT 1 cut(s) 2020
BtgI CCRYGG 2 cut(s) 1950, 2073
BtgZI GCGATG 1 cut(s) 1762
BtsCI GGATG 8 cut(s) 770, 774, 862, 1390, 2125, 2210, 2647, 2829
BtsI GCAGTG 2 cut(s) 2401, 2421
BtsIMutI CAGTG 7 cut(s) 1044, 1366, 1624, 1654, 2260, 2401, 2421
Cac8I GCNNGC 5 cut(s) 512, 723, 938, 2125, 2341
CaiI CAGNNNCTG 1 cut(s) 2810
CciI TCATGA 2 cut(s) 916, 1486
CfoI GCGC 1 cut(s) 253
Cfr10I RCCGGY 2 cut(s) 721, 2339
Cfr13I GGNCC 4 cut(s) 601, 710, 1743, 2368
ClaI ATCGAT 1 cut(s) 2020
CseI GACGC 1 cut(s) 492
Csp6I GTAC 8 cut(s) 74, 118, 166, 334, 1026, 1645, 2323, 2822
CviQI GTAC 8 cut(s) 74, 118, 166, 334, 1026, 1645, 2323, 2822
DdeI CTNAG 4 cut(s) 11, 1871, 1994, 2145
DraI TTTAAA 2 cut(s) 537, 858
DraIII CACNNNGTG 1 cut(s) 707
EaeI YGGCCR 1 cut(s) 2070
Eam1104I CTCTTC 1 cut(s) 657
EarI CTCTTC 1 cut(s) 657
EciI GGCGGA 1 cut(s) 2574
Ecl136II GAGCTC 1 cut(s) 629
Eco130I CCWWGG 3 cut(s) 183, 566, 2073
Eco24I GRGCYC 2 cut(s) 631, 1598
Eco31I GGTCTC 2 cut(s) 817, 2897
Eco47I GGWCC 4 cut(s) 601, 710, 1743, 2368
Eco47III AGCGCT 1 cut(s) 252
Eco53kI GAGCTC 1 cut(s) 629
Eco57I CTGAAG 2 cut(s) 355, 461
Eco88I CYCGRG 1 cut(s) 83
EcoICRI GAGCTC 1 cut(s) 629
EcoO109I RGGNCCY 1 cut(s) 1743
EcoRII CCWGG 4 cut(s) 989, 1598, 1618, 1704
EcoT14I CCWWGG 3 cut(s) 183, 566, 2073
EcoT22I ATGCAT 1 cut(s) 2212
EcoT38I GRGCYC 2 cut(s) 631, 1598
ErhI CCWWGG 3 cut(s) 183, 566, 2073
FalI AAGNNNNNCTT 2 cut(s) 203, 235
FaqI GGGAC 3 cut(s) 164, 1693, 2468
FauI CCCGC 1 cut(s) 726
FbaI TGATCA 1 cut(s) 913
Fnu4HI GCNGC 9 cut(s) 545, 663, 726, 729, 732, 735, 738, 741, 1206
FokI GGATG 8 cut(s) 777, 781, 849, 1377, 2132, 2197, 2634, 2816
FriOI GRGCYC 2 cut(s) 631, 1598
Fsp4HI GCNGC 9 cut(s) 545, 663, 726, 729, 732, 735, 738, 741, 1206
FspBI CTAG 5 cut(s) 417, 998, 1683, 2427, 2639
GlaI GCGC 1 cut(s) 252
GluI GCNGC 9 cut(s) 545, 663, 726, 729, 732, 735, 738, 741, 1206
GsuI CTGGAG 4 cut(s) 68, 627, 1582, 1602
HaeII RGCGCY 1 cut(s) 254
HaeIII GGCC 4 cut(s) 571, 2072, 2420, 2745
HapII CCGG 4 cut(s) 174, 722, 809, 2340
HgaI GACGC 1 cut(s) 492
HhaI GCGC 1 cut(s) 253
Hin1I GRCGYC 1 cut(s) 503
Hin6I GCGC 1 cut(s) 251
HinP1I GCGC 1 cut(s) 251
HincII GTYRAC 5 cut(s) 357, 1237, 1606, 2782, 2865
HindII GTYRAC 5 cut(s) 357, 1237, 1606, 2782, 2865
HindIII AAGCTT 2 cut(s) 1466, 1572
HpaI GTTAAC 2 cut(s) 1237, 2782
HpaII CCGG 4 cut(s) 174, 722, 809, 2340
HphI GGTGA 7 cut(s) 172, 304, 413, 813, 1706, 1832, 2607
Hpy188I TCNGA 9 cut(s) 12, 230, 374, 473, 913, 1194, 1760, 2148, 2215
Hpy99I CGWCG 1 cut(s) 319
HpyCH4III ACNGT 6 cut(s) 1048, 1241, 1361, 1649, 2264, 2495
HpyCH4IV ACGT 3 cut(s) 317, 2227, 2824
HpyF10VI GCNNNNNNNGC 9 cut(s) 259, 436, 550, 731, 734, 737, 740, 746, 2446
HpyF3I CTNAG 4 cut(s) 11, 1871, 1994, 2145
HpySE526I ACGT 3 cut(s) 317, 2227, 2824
Hsp92I GRCGYC 1 cut(s) 503
HspAI GCGC 1 cut(s) 251
KroI GCCGGC 2 cut(s) 721, 2339
KroNI GCCGGC 2 cut(s) 723, 2341
Ksp22I TGATCA 1 cut(s) 913
KspAI GTTAAC 2 cut(s) 1237, 2782
LmnI GCTCC 6 cut(s) 626, 694, 698, 811, 1601, 1621
Lsp1109I GCAGC 8 cut(s) 556, 674, 712, 715, 718, 721, 727, 749
MaeI CTAG 5 cut(s) 417, 998, 1683, 2427, 2639
MaeII ACGT 3 cut(s) 317, 2227, 2824
MaeIII GTNAC 9 cut(s) 504, 646, 1114, 1134, 1414, 1694, 1945, 2613, 2825
MfeI CAATTG 3 cut(s) 1403, 2010, 2673
MflI RGATCY 5 cut(s) 213, 1040, 1450, 1997, 2293
MhlI GDGCHC 5 cut(s) 631, 703, 1598, 1626, 1634
MlyI GAGTC 6 cut(s) 101, 352, 634, 1261, 1691, 2528
Mph1103I ATGCAT 1 cut(s) 2212
MroNI GCCGGC 2 cut(s) 721, 2339
MroXI GAANNNNTTC 4 cut(s) 119, 215, 1469, 2505
MslI CAYNNNNRTG 4 cut(s) 185, 1049, 1305, 1637
MspA1I CMGCKG 1 cut(s) 740
MspCI CTTAAG 2 cut(s) 2002, 2786
MspI CCGG 4 cut(s) 174, 722, 809, 2340
MspR9I CCNGG 5 cut(s) 175, 991, 1600, 1620, 1706
MunI CAATTG 3 cut(s) 1403, 2010, 2673
Mva1269I GAATGC 3 cut(s) 757, 1382, 2343
MvaI CCWGG 4 cut(s) 991, 1600, 1620, 1706
MwoI GCNNNNNNNGC 9 cut(s) 259, 436, 550, 731, 734, 737, 740, 746, 2446
NaeI GCCGGC 2 cut(s) 723, 2341
NciI CCSGG 1 cut(s) 175
NcoI CCATGG 1 cut(s) 2073
NgoMIV GCCGGC 2 cut(s) 721, 2339
NlaIV GGNNCC 5 cut(s) 1042, 1597, 2079, 2338, 2369
NmuCI GTSAC 4 cut(s) 504, 1694, 2613, 2825
NsiI ATGCAT 1 cut(s) 2212
NspI RCATGY 3 cut(s) 986, 1422, 2556
OliI CACNNNNGTG 1 cut(s) 185
PaeR7I CTCGAG 1 cut(s) 83
PagI TCATGA 2 cut(s) 916, 1486
PciI ACATGT 1 cut(s) 1418
PctI GAATGC 3 cut(s) 757, 1382, 2343
PdiI GCCGGC 2 cut(s) 723, 2341
PdmI GAANNNNTTC 4 cut(s) 119, 215, 1469, 2505
PfeI GAWTC 8 cut(s) 99, 196, 1220, 1917, 1933, 1965, 2149, 2687
PflMI CCANNNNNTGG 2 cut(s) 881, 1342
PkrI GCNGC 9 cut(s) 546, 664, 727, 730, 733, 736, 739, 742, 1207
PleI GAGTC 6 cut(s) 100, 352, 634, 1260, 1691, 2528
PpsI GAGTC 6 cut(s) 100, 352, 634, 1260, 1691, 2528
Ppu21I YACGTR 1 cut(s) 2825
PpuMI RGGWCCY 1 cut(s) 1743
PscI ACATGT 1 cut(s) 1418
PshBI ATTAAT 1 cut(s) 2100
Psp124BI GAGCTC 1 cut(s) 631
Psp1406I AACGTT 1 cut(s) 2227
Psp5II RGGWCCY 1 cut(s) 1743
Psp6I CCWGG 4 cut(s) 989, 1598, 1618, 1704
PspGI CCWGG 4 cut(s) 989, 1598, 1618, 1704
PspN4I GGNNCC 5 cut(s) 1042, 1597, 2079, 2338, 2369
PspPI GGNCC 4 cut(s) 601, 710, 1743, 2368
PspPPI RGGWCCY 1 cut(s) 1743
PspXI VCTCGAGB 1 cut(s) 83
PstI CTGCAG 1 cut(s) 739
PstNI CAGNNNCTG 1 cut(s) 2810
PsuI RGATCY 5 cut(s) 213, 1040, 1450, 1997, 2293
PvuII CAGCTG 1 cut(s) 740
RsaI GTAC 8 cut(s) 75, 119, 167, 335, 1027, 1646, 2324, 2823
RsaNI GTAC 8 cut(s) 74, 118, 166, 334, 1026, 1645, 2323, 2822
RseI CAYNNNNRTG 4 cut(s) 185, 1049, 1305, 1637
SacI GAGCTC 1 cut(s) 631
SatI GCNGC 9 cut(s) 545, 663, 726, 729, 732, 735, 738, 741, 1206
Sau96I GGNCC 4 cut(s) 601, 710, 1743, 2368
ScaI AGTACT 1 cut(s) 119
SchI GAGTC 6 cut(s) 101, 352, 634, 1261, 1691, 2528
ScrFI CCNGG 5 cut(s) 175, 991, 1600, 1620, 1706
SduI GDGCHC 5 cut(s) 631, 703, 1598, 1626, 1634
SfcI CTRYAG 3 cut(s) 735, 1099, 1747
Sfr274I CTCGAG 1 cut(s) 83
SinI GGWCC 4 cut(s) 601, 710, 1743, 2368
SlaI CTCGAG 1 cut(s) 83
SmiMI CAYNNNNRTG 4 cut(s) 185, 1049, 1305, 1637
SmlI CTYRAG 5 cut(s) 83, 1912, 2002, 2786, 2817
SmoI CTYRAG 5 cut(s) 83, 1912, 2002, 2786, 2817
SpeI ACTAGT 1 cut(s) 1682
SsiI CCGC 6 cut(s) 414, 719, 940, 1205, 2438, 2559
SspI AATATT 1 cut(s) 1531
SspMI CTAG 5 cut(s) 417, 998, 1683, 2427, 2639
SstI GAGCTC 1 cut(s) 631
StyD4I CCNGG 5 cut(s) 173, 989, 1598, 1618, 1704
StyI CCWWGG 3 cut(s) 183, 566, 2073
TaaI ACNGT 6 cut(s) 1048, 1241, 1361, 1649, 2264, 2495
TaiI ACGT 3 cut(s) 320, 2230, 2827
TaqI TCGA 8 cut(s) 84, 123, 222, 314, 638, 2020, 2266, 2314
TatI WGTACW 6 cut(s) 73, 117, 333, 1025, 1644, 2322
TauI GCSGC 1 cut(s) 1208
TfiI GAWTC 8 cut(s) 99, 196, 1220, 1917, 1933, 1965, 2149, 2687
TscAI CASTG 7 cut(s) 1051, 1366, 1631, 1654, 2267, 2401, 2428
TseFI GTSAC 4 cut(s) 504, 1694, 2613, 2825
TseI GCWGC 8 cut(s) 544, 662, 725, 728, 731, 734, 737, 740
Tsp45I GTSAC 4 cut(s) 504, 1694, 2613, 2825
TspGWI ACGGA 1 cut(s) 774
TspRI CASTG 7 cut(s) 1051, 1366, 1631, 1654, 2267, 2401, 2428
Van91I CCANNNNNTGG 2 cut(s) 881, 1342
Vha464I CTTAAG 2 cut(s) 2002, 2786
VneI GTGCAC 1 cut(s) 1630
VpaK11BI GGWCC 4 cut(s) 601, 710, 1743, 2368
VspI ATTAAT 1 cut(s) 2100
XapI RAATTY 3 cut(s) 59, 1838, 2329
XceI RCATGY 3 cut(s) 986, 1422, 2556
XcmI CCANNNNNNNNNTGG 1 cut(s) 1167
XhoI CTCGAG 1 cut(s) 83
XmnI GAANNNNTTC 4 cut(s) 119, 215, 1469, 2505
XspI CTAG 5 cut(s) 417, 998, 1683, 2427, 2639
ZrmI AGTACT 1 cut(s) 119
Zsp2I ATGCAT 1 cut(s) 2212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.