Rh4CG263700

Topless-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0013792
Physical Location & Seq
Forward (+)
51831360 .. 51838737
7378 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG263700.1

Sequence Viewer

Length: 3378 bp
ATGTCATCTCTGAGCAGAGAGCTGGTTTTCCTGATCCTTCAATTTCTTGAGGAGGAGAAATTCAAGGAGGCTGTACATAGGCTTGAGCAAGAGTCTGGATTCTTCTTCAACATGAAGTACTTCGAGGAGAAAGCGTTGCAGGGAGAGTGGGACGAGGTGGAGAAGTACCTGTCCGGGTTCACCAAGGTGGACGAGAATCGCTACTCTATGAAGATCTTCTTCGAGGTCAGAAAGCAGAAGTATCTCGAAGCGCTGGACAGGCAAGACAGGGCGAAAGCTGTTGAGATACTGGTGAAGGATTTGAAGGTGTTTTCGACGTTTAATGAGGACTTGTACAAGGAAATCACTCATCTGTTGACTCTTGACAACTTCAGAGAAAATGAGCAGTTGTCGAAGTATGGTGATACTAAATCCGCTAGAAGCATAATGCTGGTAGAGCTGAAGAAATTGATAGAAGCAAATCCTCTATTCCGAGAGAAGCTGATTTTCCCCACTTTGAAGGCGTCACGCTTGCGAACTTTGATCAATCAAAGTTTAAATTGGCAGCATCAGCTATGCAAAAACCCAAGGCCAAATCCTGACATTAAGACCTTATTCATGGACCACTCGTGTTCTCCTCCAAATGGAGCTCGTGCATCGACTCCAGTTACTCTTCCAGTTGCAGCTCTTACAAAGCCTTTAACTTATGCTCCTCTTGGAGCACATGGTGGACCTTTCCCGCCGGCTGCTGCTGCTGCTGCAGCTGCCAATGCAAATGCTTTAGCAGGATGGATGTCCAATGCTAATCCGTCCTTGTCTATGCAATCAGCTCCGGTCTCTGCTTCACCCCTGCCTGTTCAACTTGGTCAAGTTTCTTCTTTAAAGCACCCAAGAACACCATCAACTGGTCTTGGAATGATTGATTATCAAAGCTCTGATCATGAGCAACTAATGAAACGCCTGCGGACTGCACAATCTGTTGATGAGGTTAGCTATCCTCCTCAACATGCTTCCTGGTCGCTAGATGACCTACCGAGAAATGTATCTTGTACACTTCGTCAGGGATCCACTGTTATGAGCATGGATTTCCACCCTTCTCATCACACATTACTTGCTGTTGGCTGTAGTAATGGAGACGTTACTCTGTGGGAAGTTGGGTTACGAGAGAAGCTGGTGTCCAAGCCATTCAAAGTATGGGAAATGACTGCTTGTTCTCTGACATTTCAGGCGGCTATTGTCAAAGATTCATCAATGTCTGTTAACCGTGTATCATGGAGTCCAGACGGGAACTTAATCGGTGTTGCATTTACAAAACATTTGGTTCATCTGTATGCTTATCAAGGGTCTAATGATCTACGCCAACATTTGGAGATTGATGCCCACAGTGGAGGTGTGAATGACTTGGCATTCTCTCATCCAAACAAGCAATTGTGTGTTGTAACATGTGGAGATGATAAGCTGATAAAGGTCTGGGATCTGGCTGGAAGGAAGCTTTTCCACTTTGAAGGTCATGAAGCACCTGTTTACTCGGTTTGCCCCCACCAGAAGGAGAATATTCAGTTTATATTTTCAACTGCTGTTGATGGAAAAATAAAAGCTTGGCTGTATGACAATGTGGGCTCCAGGGTTGACTATGATGCTCCAGGGCACTGGTGCACCACAATGCTGTACAGTGATGATGGAAATAGATTGTTCTCTTGTGGGACTAGTAAAGATGGTGACTCCTTCCTGGTTGAATGGAATGAAAGTGAAGGGGCAATAAAGAGGACCTATGGTGGGTTCAGAAAGAAATCTAATGGCATCGCGCAATTTGATACCACAAGAAATCACTTTTTGGCTGTTGGTGAAGATAGCCAGATAAAATTTTGGGATATGGATAATACAAATGTTCTTCTCAGTATAGAGGCTGATGGTGGACTACCACCTGTTCCTCGCTTGAGATTCAATAAGGAAGGGAATCTGCTTGTTGTTACCACAGCTGACAATGGATTCAAGGTTCTTGCAAATGCTGATGGTCTTAGATCCTTAAGAGCAATTGAAAATCGATCTTATGAAGCATCGAGAACACCTATTGAGATGAAGGTATCTACTTCGGCCATGGTTCCAAATATCAATCCTGCCATTAATAAAGTAGATCGTATGGATGCAAGCTCTCCTGCCAGACCTACTCAGATTCTTAATGGAGTTGATCCTATGGCAAGAAGTGTAGAAAAGCGAAGACTATTAGATGATGCATCCGAGAAAAATAAACGTTGGGAACTGGCAGAGATTGTTGATCCTGTTCACTGCCGAGTGGCTACGATGCCAGATAGTAAAGATCCTGCTAACAAGGTTGCTCGACTTCTTTACACAAATTCTGGTGCCGGCATTCTGGCTCTTGGTTCAAATGGGGTCCAGAAGCTATGGAAGTGGAGAAGCAGTGAACAAAATCCAAGTGGAAAGGCCACTGCTAGTGTCGTTCCGCAACATTGGCAACCCAACAATGGTCTTTTGATGACTAATGATGTCCCTGAAAACTGTGAGGAAGCAGTTCCATGTATTGCACTCTCAAAGAATGACTCCTATGTGATGTCGGCATGTGGCGGAAAAGTGTCTCTGTTCAATATGATGACATTTAAGGTAATGACAACTTTCATGTCACCTCCACCAGCCTCAACCTTCCTAGCATTTCATCCTCTGGATAATAATATCATAGCAATTGGAACGGAAGATTCAACTATCCACATTTACAATGTTAGAGTTGATGAGGTCAAATCAAAACTGAAAGGCCACCAGAAACACATTACTGGATTAGCGTTTTCTGTTAACCTTAAGATCATGGTTTCATCAGGTGCTGATGCTCAACTATGCTTTTGGAACATGGATACGTGGGAGAAGAGGAAGTCGCTGACAATTCAACTGCCAGCCGGAAAGGCGCCTGTTGGTGACACACAAGTGCAATTCTATTCTGATCAAACTCGGTTGTTGGTATGCCATGAGACTCAACTAGCACTATATGATGCTGCCAAAGCAGAATGCATTCGACAGTGGATGCCGCAAGATGTACTGCCTGCACCAATAACATGTGCAGCATATTCCCCCAATGGTCAACTAGTCTATGCGGGTTTTACTGATGGCAATATTGGAGTATTTGATGCTGATAGTCTGAAATTAAGATGTCGTATTTCCATGTCAGTGTACTTGGCACAAGCATCCCCAAGCAGCCAAACTGTGTTCCCGCTAGCTCTTACAGCACATCTGCAGGAGCCATATCAGTTCGCCGTTGGACTGACCGATGGATCTGTTAAAGTTATAGAGCCCTCAGATGCTGAGGGGAAGTGGGGAGTTACAGTGCCTGTCGATAACGGAGCACAGAACGGCTGGACAGCAACATCTTCGTCTAACAATCCTGCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

1125

Amino Acids

124.56

Weight (kDa)

6.58

Isoelectric Point (pI)

44.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH_TPL PF17814 5 - 34 3.6e-11 LisH-like dimerisation domain
TPR1-like_2nd PF21889 37 - 161 9.5e-62 TPR1-like, CTLH-containing domain
zf_topless PF21359 164 - 204 2.8e-25 TOPLESS, zinc finger domain
Beta-prop_EML_2 PF23414 332 - 468 8.6e-08 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd PF25172 349 - 564 1.7e-10 WDR3 second beta-propeller domain
Beta-prop_RIG_2nd PF23775 359 - 500 9.5e-07 RIG second beta-propeller
Beta-prop_WDR3_1st PF25173 390 - 527 2.6e-13 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 404 - 561 1.5e-06 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 406 - 492 1.8e-08 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 408 - 623 1.1e-20 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 411 - 664 5.3e-20 THOC3 beta-propeller domain
WDR55 PF24796 412 - 527 9.2e-08 WDR55
Beta-prop_EML PF23409 449 - 653 4.5e-07 Echinoderm microtubule-associated protein first beta-propeller
WD40 PF00400 450 - 485 3.6e-06 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 458 - 530 9.2e-08 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 465 - 572 4.1e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 467 - 659 1.9e-09 Prp19 WD40 domain
WDR55 PF24796 513 - 659 6.6e-06 WDR55
Beta-prop_WDR3_1st PF25173 521 - 659 1.6e-06 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 817 - 958 2.2e-14 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 831 - 956 4e-16 WDR3 first beta-propeller domain
WDR55 PF24796 831 - 948 1.6e-08 WDR55
WD40_CDC20-Fz PF24807 832 - 957 1e-12 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 832 - 955 1e-12 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 833 - 1041 9.6e-22 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 833 - 1043 8.5e-20 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 834 - 949 9.5e-09 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 837 - 955 1.1e-13 WDR3 second beta-propeller domain
Beta-prop_IFT140_1st PF23383 850 - 949 6.9e-09 IFT140 first beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 854 - 977 9.6e-12 WDR36/Utp21 first beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 856 - 949 3.4e-08 WDR90/POC16, second beta-propeller
Beta-prop_RIG_1st PF23770 864 - 927 3.3e-06 RIG first beta-propeller
WD40_Gbeta PF25391 884 - 1039 1.9e-07 G protein beta WD-40 repeat protein
WD40_Prp19 PF24814 909 - 1044 8.1e-12 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 917 - 1048 3.6e-08 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013792)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G16830
fragaria_vesca FvH4_4g18910 FvH4_4g18910
malus_domestica MD13G1162100.v1.1 MD16G1161900.v1.1
prunus_persica Prupe.1G129400_v2.0.a1
pyrus_communis pycom13g13830
rosa_chinensis RchiOBHm_Chr4g0423811
rosa_laevigata RLG00000007530
rosa_multiflora Rmu_sc0003369.1_g000018
rosa_roxburghii Rroxscaffold_5G00365920
rosa_rugosa Rorug04G0190900
rosa_samantha Rh4AG250300 Rh4BG255600 Rh4CG263700 Rh4DG250000
rosa_wichuraiana Rw4G021430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 2339, 2893
AccB7I CCANNNNNTGG 2 cut(s) 884, 1345
AccII CGCG 1 cut(s) 1784
AciI CCGC 9 cut(s) 414, 719, 943, 1208, 2441, 2562, 3014, 3080, 3197
AclI AACGTT 1 cut(s) 2230
AclWI GGATC 9 cut(s) 28, 1038, 1051, 1461, 1995, 2162, 2249, 2291, 3265
AcoI YGGCCR 1 cut(s) 2073
AcsI RAATTY 3 cut(s) 59, 1841, 2332
AcuI CTGAAG 2 cut(s) 355, 461
AcyI GRCGYC 2 cut(s) 503, 2894
AdeI CACNNNGTG 1 cut(s) 707
AfaI GTAC 8 cut(s) 75, 119, 167, 335, 1030, 1649, 3024, 3158
AfeI AGCGCT 1 cut(s) 252
AfiI CCNNNNNNNGG 6 cut(s) 623, 884, 1345, 1525, 1755, 2462
AflII CTTAAG 2 cut(s) 2005, 2789
AflIII ACRYGT 2 cut(s) 1421, 3041
AhlI ACTAGT 2 cut(s) 1685, 3070
AjnI CCWGG 4 cut(s) 992, 1601, 1621, 1707
AleI CACNNNNGTG 2 cut(s) 185, 2912
AloI GAACNNNNNNTCC 2 cut(s) 1959, 1991
Alw21I GWGCWC 4 cut(s) 631, 703, 1637, 3331
Alw26I GTCTC 4 cut(s) 820, 1107, 2577, 2951
Alw44I GTGCAC 1 cut(s) 1633
AlwI GGATC 9 cut(s) 28, 1038, 1051, 1461, 1995, 2162, 2249, 2291, 3265
AlwNI CAGNNNCTG 3 cut(s) 2813, 3287, 3314
Aor51HI AGCGCT 1 cut(s) 252
AoxI GGCC 4 cut(s) 569, 2073, 2421, 2746
ApaLI GTGCAC 1 cut(s) 1633
ApoI RAATTY 3 cut(s) 59, 1841, 2332
AseI ATTAAT 1 cut(s) 2103
Asp700I GAANNNNTTC 5 cut(s) 119, 215, 1472, 2508, 2997
AspLEI GCGC 3 cut(s) 253, 1786, 2896
AspS9I GGNCC 4 cut(s) 601, 710, 1746, 2371
AsuC2I CCSGG 1 cut(s) 175
AsuHPI GGTGA 8 cut(s) 172, 304, 413, 816, 1709, 1835, 2610, 2915
AsuNHI GCTAGC 1 cut(s) 3199
AvaII GGWCC 4 cut(s) 601, 710, 1746, 2371
BaeGI GKGCMC 2 cut(s) 1629, 1637
BamHI GGATCC 1 cut(s) 1043
BanI GGYRCC 2 cut(s) 2339, 2893
BanII GRGCYC 3 cut(s) 631, 1601, 3279
BarI GAAGNNNNNNTAC 2 cut(s) 1122, 1154
BauI CACGAG 2 cut(s) 607, 630
BbsI GAAGAC 1 cut(s) 2203
Bbv12I GWGCWC 4 cut(s) 631, 703, 1637, 3331
BbvCI CCTCAGC 1 cut(s) 3288
BccI CCATC 9 cut(s) 762, 886, 1556, 1652, 1688, 1883, 1985, 3086, 3248
BceAI ACGGC 2 cut(s) 3224, 3352
BcgI CGANNNNNNTGC 2 cut(s) 3336, 3370
BciT130I CCWGG 4 cut(s) 994, 1603, 1623, 1709
BciVI GTATCC 1 cut(s) 2836
BclI TGATCA 3 cut(s) 522, 916, 2929
BcnI CCSGG 1 cut(s) 175
BcoDI GTCTC 4 cut(s) 820, 1107, 2577, 2951
BcuI ACTAGT 2 cut(s) 1685, 3070
BfaI CTAG 8 cut(s) 417, 1001, 1686, 2430, 2642, 2966, 3071, 3200
BfmI CTRYAG 3 cut(s) 738, 1102, 3218
BfoI RGCGCY 2 cut(s) 254, 2897
BfrI CTTAAG 2 cut(s) 2005, 2789
BfuI GTATCC 1 cut(s) 2836
BglI GCCNNNNNGGC 1 cut(s) 2891
BglII AGATCT 1 cut(s) 213
BmcAI AGTACT 1 cut(s) 119
Bme1390I CCNGG 5 cut(s) 175, 994, 1603, 1623, 1709
Bme18I GGWCC 4 cut(s) 601, 710, 1746, 2371
BmgT120I GGNCC 4 cut(s) 601, 710, 1746, 2371
BmiI GGNNCC 7 cut(s) 1045, 1600, 2082, 2341, 2372, 2895, 3225
BmrFI CCNGG 5 cut(s) 175, 994, 1603, 1623, 1709
BmtI GCTAGC 1 cut(s) 3203
BpiI GAAGAC 1 cut(s) 2203
BpmI CTGGAG 3 cut(s) 627, 1585, 1605
Bpu10I CCTNAGC 1 cut(s) 3288
BpuEI CTTGAG 3 cut(s) 68, 104, 1936
BpuMI CCSGG 1 cut(s) 175
Bsa29I ATCGAT 1 cut(s) 2023
BsaAI YACGTR 1 cut(s) 2847
BsaHI GRCGYC 2 cut(s) 503, 2894
BsaI GGTCTC 1 cut(s) 820
BsaJI CCNNGG 5 cut(s) 183, 566, 1602, 1622, 2076
BsaWI WCCGGW 1 cut(s) 811
BsaXI ACNNNNNCTCC 8 cut(s) 119, 149, 673, 703, 2383, 2413, 3294, 3324
Bsc4I CCNNNNNNNGG 6 cut(s) 623, 884, 1345, 1525, 1755, 2462
Bse118I RCCGGY 2 cut(s) 721, 2342
Bse1I ACTGG 7 cut(s) 294, 644, 656, 889, 1634, 2244, 2770
BseBI CCWGG 4 cut(s) 994, 1603, 1623, 1709
BseCI ATCGAT 1 cut(s) 2023
BseDI CCNNGG 5 cut(s) 183, 566, 1602, 1622, 2076
BseGI GGATG 8 cut(s) 773, 777, 1393, 2128, 2213, 2650, 3015, 3170
BseLI CCNNNNNNNGG 6 cut(s) 623, 884, 1345, 1525, 1755, 2462
BseMII CTCAG 4 cut(s) 1888, 2162, 3279, 3294
BseNI ACTGG 7 cut(s) 294, 644, 656, 889, 1634, 2244, 2770
BseRI GAGGAG 6 cut(s) 65, 68, 140, 606, 681, 969
BseSI GKGCMC 2 cut(s) 1629, 1637
BsgI GTGCAG 3 cut(s) 933, 3015, 3066
Bsh1236I CGCG 1 cut(s) 1784
BshFI GGCC 4 cut(s) 571, 2075, 2423, 2748
BshNI GGYRCC 2 cut(s) 2339, 2893
BshVI ATCGAT 1 cut(s) 2023
BsiHKAI GWGCWC 4 cut(s) 631, 703, 1637, 3331
BsiSI CCGG 5 cut(s) 174, 722, 812, 2343, 2886
BslFI GGGAC 3 cut(s) 164, 1696, 2471
BslI CCNNNNNNNGG 6 cut(s) 623, 884, 1345, 1525, 1755, 2462
BsmAI GTCTC 4 cut(s) 820, 1107, 2577, 2951
BsmBI CGTCTC 1 cut(s) 1107
BsmFI GGGAC 3 cut(s) 164, 1696, 2471
BsmI GAATGC 4 cut(s) 1385, 2346, 2997, 2999
BsnI GGCC 4 cut(s) 571, 2075, 2423, 2748
Bso31I GGTCTC 1 cut(s) 820
Bsp1286I GDGCHC 7 cut(s) 631, 703, 1601, 1629, 1637, 3279, 3331
Bsp1407I TGTACA 4 cut(s) 73, 333, 1028, 1647
Bsp19I CCATGG 1 cut(s) 2076
BspACI CCGC 9 cut(s) 414, 719, 943, 1208, 2441, 2562, 3014, 3080, 3197
BspANI GGCC 4 cut(s) 571, 2075, 2423, 2748
BspCNI CTCAG 4 cut(s) 1887, 2161, 3280, 3293
BspDI ATCGAT 1 cut(s) 2023
BspFNI CGCG 1 cut(s) 1784
BspHI TCATGA 2 cut(s) 919, 1489
BspLI GGNNCC 7 cut(s) 1045, 1600, 2082, 2341, 2372, 2895, 3225
BspMAI CTGCAG 2 cut(s) 742, 3222
BspOI GCTAGC 1 cut(s) 3203
BspPI GGATC 9 cut(s) 28, 1038, 1051, 1461, 1995, 2162, 2249, 2291, 3265
BspT107I GGYRCC 2 cut(s) 2339, 2893
BspTI CTTAAG 2 cut(s) 2005, 2789
BspTNI GGTCTC 1 cut(s) 820
BsrFI RCCGGY 2 cut(s) 721, 2342
BsrGI TGTACA 4 cut(s) 73, 333, 1028, 1647
BsrI ACTGG 7 cut(s) 294, 644, 656, 889, 1634, 2244, 2770
BssAI RCCGGY 2 cut(s) 721, 2342
BssECI CCNNGG 5 cut(s) 183, 566, 1602, 1622, 2076
BssNI GRCGYC 2 cut(s) 503, 2894
BssSI CACGAG 2 cut(s) 607, 630
BssT1I CCWWGG 3 cut(s) 183, 566, 2076
Bst2BI CACGAG 2 cut(s) 607, 630
Bst2UI CCWGG 4 cut(s) 994, 1603, 1623, 1709
Bst4CI ACNGT 8 cut(s) 1051, 1244, 1364, 1652, 2498, 3006, 3190, 3310
Bst6I CTCTTC 2 cut(s) 657, 2849
BstACI GRCGYC 2 cut(s) 503, 2894
BstAFI CTTAAG 2 cut(s) 2005, 2789
BstAUI TGTACA 4 cut(s) 73, 333, 1028, 1647
BstBAI YACGTR 1 cut(s) 2847
BstC8I GCNNGC 8 cut(s) 512, 723, 941, 2128, 2344, 2883, 3030, 3201
BstDEI CTNAG 6 cut(s) 11, 1874, 1997, 2148, 3280, 3288
BstDSI CCRYGG 1 cut(s) 2076
BstF5I GGATG 8 cut(s) 773, 777, 1393, 2128, 2213, 2650, 3015, 3170
BstFNI CGCG 1 cut(s) 1784
BstH2I RGCGCY 2 cut(s) 254, 2897
BstHHI GCGC 3 cut(s) 253, 1786, 2896
BstMAI GTCTC 4 cut(s) 820, 1107, 2577, 2951
BstNI CCWGG 4 cut(s) 994, 1603, 1623, 1709
BstNSI RCATGY 4 cut(s) 989, 1425, 2559, 3045
BstSCI CCNGG 5 cut(s) 173, 992, 1601, 1621, 1707
BstSFI CTRYAG 3 cut(s) 738, 1102, 3218
BstSLI GKGCMC 2 cut(s) 1629, 1637
BstUI CGCG 1 cut(s) 1784
BstV2I GAAGAC 1 cut(s) 2203
BstX2I RGATCY 6 cut(s) 213, 1043, 1453, 2000, 2296, 3257
BstXI CCANNNNNNTGG 1 cut(s) 1629
BstYI RGATCY 6 cut(s) 213, 1043, 1453, 2000, 2296, 3257
Bsu15I ATCGAT 1 cut(s) 2023
BsuI GTATCC 1 cut(s) 2836
BsuRI GGCC 4 cut(s) 571, 2075, 2423, 2748
BsuTUI ATCGAT 1 cut(s) 2023
BtgI CCRYGG 1 cut(s) 2076
BtgZI GCGATG 1 cut(s) 1765
BtsCI GGATG 8 cut(s) 773, 777, 1393, 2128, 2213, 2650, 3015, 3170
BtsI GCAGTG 3 cut(s) 2263, 2404, 2424
Cac8I GCNNGC 8 cut(s) 512, 723, 941, 2128, 2344, 2883, 3030, 3201
CaiI CAGNNNCTG 3 cut(s) 2813, 3287, 3314
CciI TCATGA 2 cut(s) 919, 1489
CfoI GCGC 3 cut(s) 253, 1786, 2896
Cfr10I RCCGGY 2 cut(s) 721, 2342
Cfr13I GGNCC 4 cut(s) 601, 710, 1746, 2371
ClaI ATCGAT 1 cut(s) 2023
CseI GACGC 1 cut(s) 492
Csp6I GTAC 8 cut(s) 74, 118, 166, 334, 1029, 1648, 3023, 3157
CviQI GTAC 8 cut(s) 74, 118, 166, 334, 1029, 1648, 3023, 3157
DdeI CTNAG 6 cut(s) 11, 1874, 1997, 2148, 3280, 3288
DinI GGCGCC 1 cut(s) 2895
DraI TTTAAA 2 cut(s) 537, 861
DraIII CACNNNGTG 1 cut(s) 707
EaeI YGGCCR 1 cut(s) 2073
Eam1104I CTCTTC 2 cut(s) 657, 2849
EarI CTCTTC 2 cut(s) 657, 2849
EciI GGCGGA 1 cut(s) 2577
Ecl136II GAGCTC 1 cut(s) 629
Eco130I CCWWGG 3 cut(s) 183, 566, 2076
Eco24I GRGCYC 3 cut(s) 631, 1601, 3279
Eco31I GGTCTC 1 cut(s) 820
Eco47I GGWCC 4 cut(s) 601, 710, 1746, 2371
Eco47III AGCGCT 1 cut(s) 252
Eco53kI GAGCTC 1 cut(s) 629
Eco57I CTGAAG 2 cut(s) 355, 461
EcoICRI GAGCTC 1 cut(s) 629
EcoO109I RGGNCCY 1 cut(s) 1746
EcoRII CCWGG 4 cut(s) 992, 1601, 1621, 1707
EcoT14I CCWWGG 3 cut(s) 183, 566, 2076
EcoT22I ATGCAT 2 cut(s) 2215, 2999
EcoT38I GRGCYC 3 cut(s) 631, 1601, 3279
EgeI GGCGCC 1 cut(s) 2895
EheI GGCGCC 1 cut(s) 2895
ErhI CCWWGG 3 cut(s) 183, 566, 2076
Esp3I CGTCTC 1 cut(s) 1107
FalI AAGNNNNNCTT 2 cut(s) 203, 235
FaqI GGGAC 3 cut(s) 164, 1696, 2471
FauI CCCGC 3 cut(s) 726, 3073, 3204
FbaI TGATCA 3 cut(s) 522, 916, 2929
FokI GGATG 8 cut(s) 780, 784, 1380, 2135, 2200, 2637, 3022, 3157
FriOI GRGCYC 3 cut(s) 631, 1601, 3279
FspBI CTAG 8 cut(s) 417, 1001, 1686, 2430, 2642, 2966, 3071, 3200
GlaI GCGC 3 cut(s) 252, 1785, 2895
GsuI CTGGAG 3 cut(s) 627, 1585, 1605
HaeII RGCGCY 2 cut(s) 254, 2897
HaeIII GGCC 4 cut(s) 571, 2075, 2423, 2748
HapII CCGG 5 cut(s) 174, 722, 812, 2343, 2886
HgaI GACGC 1 cut(s) 492
HhaI GCGC 3 cut(s) 253, 1786, 2896
Hin1I GRCGYC 2 cut(s) 503, 2894
Hin6I GCGC 3 cut(s) 251, 1784, 2894
HinP1I GCGC 3 cut(s) 251, 1784, 2894
HincII GTYRAC 5 cut(s) 357, 1240, 1609, 2785, 3068
HindII GTYRAC 5 cut(s) 357, 1240, 1609, 2785, 3068
HindIII AAGCTT 2 cut(s) 1469, 1575
HpaI GTTAAC 2 cut(s) 1240, 2785
HpaII CCGG 5 cut(s) 174, 722, 812, 2343, 2886
HphI GGTGA 8 cut(s) 172, 304, 413, 816, 1709, 1835, 2610, 2915
Hpy99I CGWCG 1 cut(s) 319
HpyCH4III ACNGT 8 cut(s) 1051, 1244, 1364, 1652, 2498, 3006, 3190, 3310
HpyCH4IV ACGT 4 cut(s) 317, 1116, 2230, 2846
HpyF3I CTNAG 6 cut(s) 11, 1874, 1997, 2148, 3280, 3288
HpySE526I ACGT 4 cut(s) 317, 1116, 2230, 2846
Hsp92I GRCGYC 2 cut(s) 503, 2894
HspAI GCGC 3 cut(s) 251, 1784, 2894
KasI GGCGCC 1 cut(s) 2893
KroI GCCGGC 2 cut(s) 721, 2342
KroNI GCCGGC 2 cut(s) 723, 2344
Ksp22I TGATCA 3 cut(s) 522, 916, 2929
KspAI GTTAAC 2 cut(s) 1240, 2785
LmnI GCTCC 8 cut(s) 626, 694, 698, 814, 1604, 1624, 3223, 3326
MaeI CTAG 8 cut(s) 417, 1001, 1686, 2430, 2642, 2966, 3071, 3200
MaeII ACGT 4 cut(s) 317, 1116, 2230, 2846
MfeI CAATTG 3 cut(s) 1406, 2013, 2676
MflI RGATCY 6 cut(s) 213, 1043, 1453, 2000, 2296, 3257
MhlI GDGCHC 7 cut(s) 631, 703, 1601, 1629, 1637, 3279, 3331
Mly113I GGCGCC 1 cut(s) 2894
MlyI GAGTC 7 cut(s) 101, 352, 634, 1264, 1694, 2531, 2953
MmeI TCCRAC 1 cut(s) 3223
Mph1103I ATGCAT 2 cut(s) 2215, 2999
MroNI GCCGGC 2 cut(s) 721, 2342
MroXI GAANNNNTTC 5 cut(s) 119, 215, 1472, 2508, 2997
MslI CAYNNNNRTG 6 cut(s) 185, 1052, 1308, 1640, 2912, 3152
MspA1I CMGCKG 2 cut(s) 743, 1958
MspCI CTTAAG 2 cut(s) 2005, 2789
MspI CCGG 5 cut(s) 174, 722, 812, 2343, 2886
MspR9I CCNGG 5 cut(s) 175, 994, 1603, 1623, 1709
MunI CAATTG 3 cut(s) 1406, 2013, 2676
Mva1269I GAATGC 4 cut(s) 1385, 2346, 2997, 2999
MvaI CCWGG 4 cut(s) 994, 1603, 1623, 1709
MvnI CGCG 1 cut(s) 1784
NaeI GCCGGC 2 cut(s) 723, 2344
NarI GGCGCC 1 cut(s) 2894
NciI CCSGG 1 cut(s) 175
NcoI CCATGG 1 cut(s) 2076
NgoMIV GCCGGC 2 cut(s) 721, 2342
NheI GCTAGC 1 cut(s) 3199
NlaIV GGNNCC 7 cut(s) 1045, 1600, 2082, 2341, 2372, 2895, 3225
NmeAIII GCCGAG 1 cut(s) 2294
NmuCI GTSAC 4 cut(s) 504, 1697, 2616, 2903
NsiI ATGCAT 2 cut(s) 2215, 2999
NspI RCATGY 4 cut(s) 989, 1425, 2559, 3045
OliI CACNNNNGTG 2 cut(s) 185, 2912
PagI TCATGA 2 cut(s) 919, 1489
PciI ACATGT 2 cut(s) 1421, 3041
PctI GAATGC 4 cut(s) 1385, 2346, 2997, 2999
PdiI GCCGGC 2 cut(s) 723, 2344
PdmI GAANNNNTTC 5 cut(s) 119, 215, 1472, 2508, 2997
PfeI GAWTC 8 cut(s) 99, 196, 1223, 1920, 1936, 1968, 2152, 2690
PflMI CCANNNNNTGG 2 cut(s) 884, 1345
PleI GAGTC 7 cut(s) 100, 352, 634, 1263, 1694, 2531, 2953
PluTI GGCGCC 1 cut(s) 2897
PpsI GAGTC 7 cut(s) 100, 352, 634, 1263, 1694, 2531, 2953
Ppu21I YACGTR 1 cut(s) 2847
PpuMI RGGWCCY 1 cut(s) 1746
PscI ACATGT 2 cut(s) 1421, 3041
PshBI ATTAAT 1 cut(s) 2103
Psp124BI GAGCTC 1 cut(s) 631
Psp1406I AACGTT 1 cut(s) 2230
Psp5II RGGWCCY 1 cut(s) 1746
Psp6I CCWGG 4 cut(s) 992, 1601, 1621, 1707
PspGI CCWGG 4 cut(s) 992, 1601, 1621, 1707
PspN4I GGNNCC 7 cut(s) 1045, 1600, 2082, 2341, 2372, 2895, 3225
PspPI GGNCC 4 cut(s) 601, 710, 1746, 2371
PspPPI RGGWCCY 1 cut(s) 1746
PstI CTGCAG 2 cut(s) 742, 3222
PstNI CAGNNNCTG 3 cut(s) 2813, 3287, 3314
PsuI RGATCY 6 cut(s) 213, 1043, 1453, 2000, 2296, 3257
PvuII CAGCTG 2 cut(s) 743, 1958
RsaI GTAC 8 cut(s) 75, 119, 167, 335, 1030, 1649, 3024, 3158
RsaNI GTAC 8 cut(s) 74, 118, 166, 334, 1029, 1648, 3023, 3157
RseI CAYNNNNRTG 6 cut(s) 185, 1052, 1308, 1640, 2912, 3152
SacI GAGCTC 1 cut(s) 631
Sau96I GGNCC 4 cut(s) 601, 710, 1746, 2371
ScaI AGTACT 1 cut(s) 119
SchI GAGTC 7 cut(s) 101, 352, 634, 1264, 1694, 2531, 2953
ScrFI CCNGG 5 cut(s) 175, 994, 1603, 1623, 1709
SduI GDGCHC 7 cut(s) 631, 703, 1601, 1629, 1637, 3279, 3331
SfcI CTRYAG 3 cut(s) 738, 1102, 3218
SfoI GGCGCC 1 cut(s) 2895
SinI GGWCC 4 cut(s) 601, 710, 1746, 2371
SmiMI CAYNNNNRTG 6 cut(s) 185, 1052, 1308, 1640, 2912, 3152
SmlI CTYRAG 5 cut(s) 47, 83, 1915, 2005, 2789
SmoI CTYRAG 5 cut(s) 47, 83, 1915, 2005, 2789
SpeI ACTAGT 2 cut(s) 1685, 3070
SsiI CCGC 9 cut(s) 414, 719, 943, 1208, 2441, 2562, 3014, 3080, 3197
SspDI GGCGCC 1 cut(s) 2893
SspI AATATT 2 cut(s) 1534, 3100
SspMI CTAG 8 cut(s) 417, 1001, 1686, 2430, 2642, 2966, 3071, 3200
SstI GAGCTC 1 cut(s) 631
StyD4I CCNGG 5 cut(s) 173, 992, 1601, 1621, 1707
StyI CCWWGG 3 cut(s) 183, 566, 2076
TaaI ACNGT 8 cut(s) 1051, 1244, 1364, 1652, 2498, 3006, 3190, 3310
TaiI ACGT 4 cut(s) 320, 1119, 2233, 2849
TaqII GACCGA 1 cut(s) 3266
TatI WGTACW 7 cut(s) 73, 117, 333, 1028, 1647, 3022, 3156
TauI GCSGC 2 cut(s) 1211, 3016
TfiI GAWTC 8 cut(s) 99, 196, 1223, 1920, 1936, 1968, 2152, 2690
TseFI GTSAC 4 cut(s) 504, 1697, 2616, 2903
Tsp45I GTSAC 4 cut(s) 504, 1697, 2616, 2903
TspGWI ACGGA 3 cut(s) 777, 2699, 3339
Van91I CCANNNNNTGG 2 cut(s) 884, 1345
Vha464I CTTAAG 2 cut(s) 2005, 2789
VneI GTGCAC 1 cut(s) 1633
VpaK11BI GGWCC 4 cut(s) 601, 710, 1746, 2371
VspI ATTAAT 1 cut(s) 2103
XapI RAATTY 3 cut(s) 59, 1841, 2332
XceI RCATGY 4 cut(s) 989, 1425, 2559, 3045
XcmI CCANNNNNNNNNTGG 1 cut(s) 1170
XmnI GAANNNNTTC 5 cut(s) 119, 215, 1472, 2508, 2997
XspI CTAG 8 cut(s) 417, 1001, 1686, 2430, 2642, 2966, 3071, 3200
ZrmI AGTACT 1 cut(s) 119
Zsp2I ATGCAT 2 cut(s) 2215, 2999
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.