MD16G1161900.v1.1

Topless-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0013792
Physical Location & Seq
Reverse (-)
13206302 .. 13213818
7517 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1161900.v1.1.491

Sequence Viewer

Length: 3414 bp
ATGTCGTCTTTGAGCAGAGAACTGGTGTTCCTGATCCTGCAATTTCTGGAGGAAGAGAAATTCAAGGAGTCCGTACACAGGCTGGAGCAGGAATCGGGATACTTCTTCAACATGAAGTACTTCGAGGAGAAGGCGCTGGCGGGAGAGTGGGATGAGGTCGAGAAGTACCTCTCTGGGTTCACCAAGGTCGATGAGAATCGCTATTCGATGAAGATTTTCTTTGAGGTTCGAAAGCAGAAGTATCTCGAGGCGCTTGACAGGAATGACAGAGCCAAGGCAGTTGAGATACTGGTGAAGGATTTGAAAGTGTTCTCAACGTTTAATGAAGAATTGTACAAGGAAATTACGCATCTTTTGACTCTCGAAAACTTCAGAGAAAATGAGCAGTTATCGAAATATGGTGATACTAAGTCAGCTAGAAGCATAATGCTAGTAGAGCTTAAAAAGTTGATAGAAGCAAATCCTCTTTTCCGTGAGAAGCTTGGTTTACCCACTCTAAAGGCTTCACGCTTGCGAACTCTAATTAATCAAAGTCTAAATTGGCAGCACCAGCTGTGCAAGAACCCAAGGCCAAATCCTGACATTAAAACCTTATTCATGGATCACTCGTGTTCTCCGACAAATGGGGCTCGTGCATCCACGCCAGTTACTCTTCCAGTTGCAGCTCTTGCGAAGCCAACTTATGCTCCTCTAGGAGCACATGGTGGACCTTTTCCGCCGGCAGCTGCAGCTGCAGCTAATGCAAATTTAGCAGGGTGGATGTCAAATGCTAATCCTTCCTTGTCTGTGCAATCAGCTGTTGTTGCCGCTTCACCCTTCCCTGTTCAACCCAGTCAAGTTTCTGCTTTAAAGCATCCAAGAACACCGTCAAATGCTCTTGGAATGGGTGATTATCCAAACTCAGATCACGAGCAACTAATGAAACGCCTACGATCTGCACAACCTGTTGATGAGGTCAGTTATCCTCCCCCTCCCCCTCCCCAGCATGCTTCCTGGTCACTAGATGACCTACCGAGAACTGTAGCATGCACTCTTCATCAAGGGTCCACTGTGATAAGCATGGATTTCCACCCTTCTCACCACACATTACTTGCTGTTGGCTGTAGTAATGGCGAAATTGCACTATGGGAAGTTGGGATGCGAGAGAGGCTAGTGTCAAAGCCATTCAAAGTATGGAATATGACTACTTGTTCTAATGCATTTCAGGTTGCTATGGTCAAAGATACATCGATATCTGTAAGCCGTGTATCATGGAATCAAGATGGAAGCTTAATTGGTGTTGCATTTACCAAACACTTGGTTCATTTGTACGCTTATCAAGGACCTAATGATCTACGGCAACATTGGGAGATTGACGCTCACAGTGGTAGTGTGAATGACTTAGCCTTTTCTCTTCCAAACAAGCAATTATGTCTTATAACGTGTGGGGAGGATATGCTAATAAAGGTTTGGGATTTGGCTGGAAGAAATCTATTCAGCTTTGAAGGTCATGAAGCACCTGTTTACTCGGTTTGCCCCCACCAGAAGGAGAATATTCAGTTCATATTTTCAACTGCTGTTGATGGGAAAATAAAAGCTTGGCTGTATGACAATGTGGGCTCCAGGGTTGACTATGATGCTCCTGGGCAGTGGTGCACCACAATGCTCTACAGTGATGACGGAAATAGATTATTCTCTTGTGGGACTAGTAAAGATGGTGAATCTTTCCTCGTTGAATGGAATGAAAGTGAAGGGGCAATAAAGAGGACATATAACGGATTCCGAAAAAAATCTTCTGGCATAGTGCAGTTTGATACAACAAAAAATCACTTTTTGGCTGTTGGTGAAGATAACCAGATAAAGTTTTGGGATATGGATAGTACAAATGTTCTTACTAGTACAGAAGCTGAGGGTGGACTTCTGACTCTTCCCCGTTTGAGATTCAATAAGGAAGGAAATTTGCTTGCTGCTACCACGGTTGACAACGGAATCAAAGTTCTTGTTAATGCTGAGGGTCGCAGATCCTTAAGAGCAATTGAAACTCGATCTTATGAAGCATCCAGAGCACCTATTGAGATGAAGGTATCTGGTTCTTCTATGGTTCCGAACATCAACCCAACCATTAATAAAGTGGACCGTATGGATACAAGCTCTCCTGCCAGACCGACTCATATTCTTAATGGAGCTGATTCTATGGCAAGGGCCGAGTCTATGGCAAGGGCCGATTCTATGGCAAGAAGCCTGGAAAAGAGAAGAAGTTTAGATGATGTATCTGACAAAATTAAACGTTGGGAACTGGCGGAGATTGTTGATCCGGTTCAGTGTCGGGTGGCTACTATGCCAGAAAGTAAAGATCCTGCTAACAAGGTTGCTCGACTTCTTTACACAAATTCTGGTTCTGGCATTCTGGCACTCGGTTCAAATGGGGTGCAGAAGCTATGGAAGTGGAGCCGCAATGAACAAAATCCAAGTGGGAAGGCCACAGCAAGTGTTGTTCCCCAACATTGGCAACCCAACAGTGGTCTTTTAATGACTAATGATGTCTCAGAAAATGTTGAAGAAGCGGTTCCATGCATTGCACTCTCGAAAAATGATTCTTATGTGATGTCAGCCTGTGGAGGAAAAGTGTCTCTGTTCAATATGATGACTTTTAAGGTGATGACAACTTTCATGCCACCTCCACCAGTCTCAACCTACCTGTCATTTCATCCTCTGGATAATAACATCATAGCAATAGGAATGGAAGATTCAACTATCCACATTTACAATGTTCGACTTGATGAGGTCAAAGCAAAGCTGAAAGGCCACCAGAAGCACATTACTGGTTTAGCATTTTCTATCAGCCATAAACTCATGGTTTCATCAGGTGCCGACGCTCAACTATGCTTCTGGAACATGGATACATGGGATAAGAGGAAATCGGTTCCACTTCAGTTGCCTGCTGGAAAGGCGCCTGTTGGTGACACGCATGTGCAATTCTATTCTGATCAAGTCCGATTGTTGGTATACCATGAGACTCAACTAGCACTATATGATGCGGCCAAGTCGGAGTGCATTCGGCAGTGGACGCCACAAGATGTACTGCCTGCGCCCATATCTTGTGCAGCGTATTCCTCCAGTAGTCAACTAGTTTATGCGGCTTTTACTGATGGTAATATTGGAGTATTTGATGCTGATAGTCTGAAACTAAGATGCCGTATTGCCATGTCGGTGTACTTACCACAACCATCATCAAACAGCCCAACCGTGTACCCGCTGGCTCTAACAGCACATCTGCAGGAGCCGTGCCAATTCGCTGTTGGACTGACAGATGGATCTGTTAAAGTTATAGAACCCTCGGAATCTGAGGGGAAGTGGGGAGTTTTGGTGCCTGTTGATAACGGAGCACAAAACGGGTGGACAGCAACATCTTCGAGTAACAATCCAGCATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

1138

Amino Acids

126.0

Weight (kDa)

6.51

Isoelectric Point (pI)

44.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH_TPL PF17814 5 - 33 3.5e-11 LisH-like dimerisation domain
TPR1-like_2nd PF21889 37 - 161 2.2e-61 TPR1-like, CTLH-containing domain
zf_topless PF21359 164 - 204 2.9e-25 TOPLESS, zinc finger domain
Beta-prop_EML_2 PF23414 332 - 465 6.7e-06 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 342 - 527 3.5e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd PF25172 346 - 564 3.4e-07 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 389 - 504 3e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 390 - 562 1.7e-13 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 390 - 487 4.6e-07 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 401 - 560 8.5e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 410 - 624 2.6e-17 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 412 - 673 3.3e-20 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 458 - 530 6.3e-08 WDHD1 first WD40 domain
WDR55 PF24796 513 - 660 1.1e-06 WDR55
Beta-prop_WDR3_1st PF25173 521 - 659 2.6e-06 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 829 - 1038 4.1e-13 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 840 - 1055 1.7e-15 WDHD1 first WD40 domain
WDR55 PF24796 840 - 960 1.2e-09 WDR55
WD40_CDC20-Fz PF24807 841 - 965 1.2e-11 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 842 - 964 5.1e-15 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 843 - 966 7.1e-12 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 846 - 1053 6.1e-17 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 846 - 961 3.9e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 849 - 967 3.8e-12 WDR3 second beta-propeller domain
Beta-prop_IFT140_1st PF23383 862 - 966 6.7e-09 IFT140 first beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 866 - 961 1.7e-10 WDR36/Utp21 first beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 868 - 960 6.2e-08 WDR90/POC16, second beta-propeller
WD40_Gbeta PF25391 891 - 1051 1e-05 G protein beta WD-40 repeat protein
Beta-prop_THOC3 PF25174 899 - 1057 5.9e-10 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013792)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G16830
fragaria_vesca FvH4_4g18910 FvH4_4g18910
malus_domestica MD13G1162100.v1.1 MD16G1161900.v1.1
prunus_persica Prupe.1G129400_v2.0.a1
pyrus_communis pycom13g13830
rosa_chinensis RchiOBHm_Chr4g0423811
rosa_laevigata RLG00000007530
rosa_multiflora Rmu_sc0003369.1_g000018
rosa_roxburghii Rroxscaffold_5G00365920
rosa_rugosa Rorug04G0190900
rosa_samantha Rh4AG250300 Rh4BG255600 Rh4CG263700 Rh4DG250000
rosa_wichuraiana Rw4G021430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1418
AccB1I GGYRCC 3 cut(s) 2846, 2929, 3346
AccI GTMKAC 1 cut(s) 2985
AciI CCGC 9 cut(s) 140, 716, 807, 2279, 2431, 2543, 3017, 3116, 3233
AclI AACGTT 2 cut(s) 317, 2266
AclWI GGATC 6 cut(s) 28, 609, 1995, 2285, 2327, 3301
AcoI YGGCCR 1 cut(s) 3018
AcsI RAATTY 4 cut(s) 59, 745, 1936, 2368
AcuI CTGAAG 2 cut(s) 355, 2894
AcyI GRCGYC 2 cut(s) 2930, 3047
AdeI CACNNNGTG 1 cut(s) 704
AfiI CCNNNNNNNGG 6 cut(s) 78, 623, 1525, 2484, 2498, 2980
AflII CTTAAG 1 cut(s) 2005
AflIII ACRYGT 1 cut(s) 1421
AhlI ACTAGT 3 cut(s) 1685, 1874, 3106
AjnI CCWGG 4 cut(s) 992, 1601, 1621, 2220
AleI CACNNNNGTG 1 cut(s) 2948
AloI GAACNNNNNNTCC 4 cut(s) 12, 44, 1959, 1991
Alw21I GWGCWC 4 cut(s) 700, 1637, 2047, 3367
Alw26I GTCTC 4 cut(s) 2527, 2613, 2671, 2987
Alw44I GTGCAC 1 cut(s) 1633
AlwI GGATC 6 cut(s) 28, 609, 1995, 2285, 2327, 3301
AlwNI CAGNNNCTG 1 cut(s) 1886
Ama87I CYCGRG 1 cut(s) 245
AoxI GGCC 6 cut(s) 569, 2181, 2199, 2457, 2782, 3018
ApaLI GTGCAC 1 cut(s) 1633
ApeKI GCWGC 9 cut(s) 544, 662, 722, 725, 728, 731, 734, 1946, 3083
ApoI RAATTY 4 cut(s) 59, 745, 1936, 2368
AseI ATTAAT 2 cut(s) 525, 2103
Asp700I GAANNNNTTC 4 cut(s) 119, 215, 308, 2544
AspLEI GCGC 4 cut(s) 136, 253, 2932, 3070
AspS9I GGNCC 6 cut(s) 707, 1044, 1322, 2113, 2181, 2199
AsuII TTCGAA 1 cut(s) 229
AvaI CYCGRG 1 cut(s) 245
AvaII GGWCC 4 cut(s) 707, 1044, 1322, 2113
BaeGI GKGCMC 1 cut(s) 1637
BanI GGYRCC 3 cut(s) 2846, 2929, 3346
BanII GRGCYC 2 cut(s) 631, 1601
BauI CACGAG 3 cut(s) 607, 630, 908
Bbv12I GWGCWC 4 cut(s) 700, 1637, 2047, 3367
BbvCI CCTCAGC 2 cut(s) 1887, 1989
BbvI GCAGC 9 cut(s) 556, 674, 712, 718, 734, 740, 746, 1933, 3095
BccI CCATC 6 cut(s) 1256, 1556, 1688, 3122, 3214, 3284
BceAI ACGGC 4 cut(s) 1227, 1352, 3159, 3247
BcgI CGANNNNNNTGC 2 cut(s) 3372, 3406
BciT130I CCWGG 4 cut(s) 994, 1603, 1623, 2222
BciVI GTATCC 3 cut(s) 92, 2116, 2872
BclI TGATCA 1 cut(s) 2965
BcoDI GTCTC 4 cut(s) 2527, 2613, 2671, 2987
BcuI ACTAGT 3 cut(s) 1685, 1874, 3106
BfaI CTAG 9 cut(s) 417, 431, 692, 1001, 1151, 1686, 1875, 3002, 3107
BfmI CTRYAG 6 cut(s) 726, 732, 1020, 1102, 1648, 3254
BfoI RGCGCY 3 cut(s) 137, 254, 2933
BfrI CTTAAG 1 cut(s) 2005
BfuI GTATCC 3 cut(s) 92, 2116, 2872
BmcAI AGTACT 1 cut(s) 119
Bme1390I CCNGG 4 cut(s) 994, 1603, 1623, 2222
Bme18I GGWCC 4 cut(s) 707, 1044, 1322, 2113
BmeT110I CYCGRG 1 cut(s) 245
BmgT120I GGNCC 6 cut(s) 707, 1044, 1322, 2113, 2181, 2199
BmrFI CCNGG 4 cut(s) 994, 1603, 1623, 2222
BmrI ACTGGG 1 cut(s) 825
BmsI GCATC 9 cut(s) 358, 644, 862, 1128, 1606, 2045, 3004, 3139, 3161
BmuI ACTGGG 1 cut(s) 825
BpmI CTGGAG 4 cut(s) 68, 104, 1585, 3079
Bpu10I CCTNAGC 2 cut(s) 1887, 1989
Bpu14I TTCGAA 1 cut(s) 229
Bsa29I ATCGAT 1 cut(s) 1229
BsaBI GATNNNNATC 1 cut(s) 195
BsaHI GRCGYC 2 cut(s) 2930, 3047
BsaJI CCNNGG 7 cut(s) 183, 273, 566, 1602, 1622, 1953, 3315
BsaWI WCCGGW 1 cut(s) 2293
BsaXI ACNNNNNCTCC 8 cut(s) 670, 700, 2116, 2146, 2589, 2619, 3330, 3360
Bsc4I CCNNNNNNNGG 6 cut(s) 78, 623, 1525, 2484, 2498, 2980
Bse118I RCCGGY 1 cut(s) 718
Bse1I ACTGG 9 cut(s) 27, 294, 644, 656, 831, 2280, 2663, 2806, 3096
Bse3DI GCAATG 2 cut(s) 2440, 2553
Bse8I GATNNNNATC 1 cut(s) 195
BseBI CCWGG 4 cut(s) 994, 1603, 1623, 2222
BseCI ATCGAT 1 cut(s) 1229
BseDI CCNNGG 7 cut(s) 183, 273, 566, 1602, 1622, 1953, 3315
BseGI GGATG 7 cut(s) 157, 635, 765, 853, 1143, 2036, 2686
BseJI GATNNNNATC 1 cut(s) 195
BseLI CCNNNNNNNGG 6 cut(s) 78, 623, 1525, 2484, 2498, 2980
BseMI GCAATG 2 cut(s) 2440, 2553
BseMII CTCAG 5 cut(s) 915, 1878, 1980, 2538, 3315
BseNI ACTGG 9 cut(s) 27, 294, 644, 656, 831, 2280, 2663, 2806, 3096
BseRI GAGGAG 2 cut(s) 140, 678
BseSI GKGCMC 1 cut(s) 1637
BseXI GCAGC 9 cut(s) 556, 674, 712, 718, 734, 740, 746, 1933, 3095
BseYI CCCAGC 1 cut(s) 981
BsgI GTGCAG 4 cut(s) 921, 1805, 2429, 3102
BshFI GGCC 6 cut(s) 571, 2183, 2201, 2459, 2784, 3020
BshNI GGYRCC 3 cut(s) 2846, 2929, 3346
BshVI ATCGAT 1 cut(s) 1229
BsiHKAI GWGCWC 4 cut(s) 700, 1637, 2047, 3367
BsiHKCI CYCGRG 1 cut(s) 245
BsiSI CCGG 2 cut(s) 719, 2294
BslFI GGGAC 1 cut(s) 1696
BslI CCNNNNNNNGG 6 cut(s) 78, 623, 1525, 2484, 2498, 2980
BsmAI GTCTC 4 cut(s) 2527, 2613, 2671, 2987
BsmFI GGGAC 1 cut(s) 1696
BsmI GAATGC 2 cut(s) 2382, 3033
BsnI GGCC 6 cut(s) 571, 2183, 2201, 2459, 2784, 3020
BsoBI CYCGRG 1 cut(s) 245
Bsp119I TTCGAA 1 cut(s) 229
Bsp1286I GDGCHC 6 cut(s) 631, 700, 1601, 1637, 2047, 3367
Bsp1407I TGTACA 1 cut(s) 333
BspACI CCGC 9 cut(s) 140, 716, 807, 2279, 2431, 2543, 3017, 3116, 3233
BspANI GGCC 6 cut(s) 571, 2183, 2201, 2459, 2784, 3020
BspCNI CTCAG 5 cut(s) 914, 1879, 1981, 2537, 3316
BspDI ATCGAT 1 cut(s) 1229
BspHI TCATGA 1 cut(s) 1489
BspMAI CTGCAG 3 cut(s) 730, 736, 3258
BspPI GGATC 6 cut(s) 28, 609, 1995, 2285, 2327, 3301
BspT104I TTCGAA 1 cut(s) 229
BspT107I GGYRCC 3 cut(s) 2846, 2929, 3346
BspTI CTTAAG 1 cut(s) 2005
BsrDI GCAATG 2 cut(s) 2440, 2553
BsrFI RCCGGY 1 cut(s) 718
BsrGI TGTACA 1 cut(s) 333
BsrI ACTGG 9 cut(s) 27, 294, 644, 656, 831, 2280, 2663, 2806, 3096
BssAI RCCGGY 1 cut(s) 718
BssECI CCNNGG 7 cut(s) 183, 273, 566, 1602, 1622, 1953, 3315
BssNAI GTATAC 1 cut(s) 2986
BssNI GRCGYC 2 cut(s) 2930, 3047
BssSI CACGAG 3 cut(s) 607, 630, 908
BssT1I CCWWGG 3 cut(s) 183, 273, 566
Bst1107I GTATAC 1 cut(s) 2986
Bst2BI CACGAG 3 cut(s) 607, 630, 908
Bst2UI CCWGG 4 cut(s) 994, 1603, 1623, 2222
Bst4CI ACNGT 9 cut(s) 867, 1021, 1051, 1364, 1652, 1957, 2117, 2498, 3226
Bst6I CTCTTC 5 cut(s) 48, 657, 1038, 1398, 1911
BstACI GRCGYC 2 cut(s) 2930, 3047
BstAFI CTTAAG 1 cut(s) 2005
BstAPI GCANNNNNTGC 2 cut(s) 668, 740
BstAUI TGTACA 1 cut(s) 333
BstBI TTCGAA 1 cut(s) 229
BstC8I GCNNGC 9 cut(s) 138, 512, 720, 987, 1027, 1944, 2919, 3066, 3237
BstDEI CTNAG 9 cut(s) 408, 901, 1381, 1887, 1989, 2524, 3167, 3324, 3411
BstDSI CCRYGG 1 cut(s) 1953
BstF5I GGATG 7 cut(s) 157, 635, 765, 853, 1143, 2036, 2686
BstH2I RGCGCY 3 cut(s) 137, 254, 2933
BstHHI GCGC 4 cut(s) 136, 253, 2932, 3070
BstMAI GTCTC 4 cut(s) 2527, 2613, 2671, 2987
BstNI CCWGG 4 cut(s) 994, 1603, 1623, 2222
BstNSI RCATGY 3 cut(s) 989, 1029, 2951
BstSCI CCNGG 4 cut(s) 992, 1601, 1621, 2220
BstSFI CTRYAG 6 cut(s) 726, 732, 1020, 1102, 1648, 3254
BstSLI GKGCMC 1 cut(s) 1637
BstV1I GCAGC 9 cut(s) 556, 674, 712, 718, 734, 740, 746, 1933, 3095
BstX2I RGATCY 3 cut(s) 2000, 2332, 3293
BstXI CCANNNNNNTGG 1 cut(s) 1297
BstYI RGATCY 3 cut(s) 2000, 2332, 3293
BstZ17I GTATAC 1 cut(s) 2986
Bsu15I ATCGAT 1 cut(s) 1229
BsuI GTATCC 3 cut(s) 92, 2116, 2872
BsuRI GGCC 6 cut(s) 571, 2183, 2201, 2459, 2784, 3020
BsuTUI ATCGAT 1 cut(s) 1229
BtgI CCRYGG 1 cut(s) 1953
BtsCI GGATG 7 cut(s) 157, 635, 765, 853, 1143, 2036, 2686
BtsI GCAGTG 2 cut(s) 1634, 3047
BtsIMutI CAGTG 7 cut(s) 1047, 1369, 1634, 1657, 2306, 2503, 3047
Cac8I GCNNGC 9 cut(s) 138, 512, 720, 987, 1027, 1944, 2919, 3066, 3237
CaiI CAGNNNCTG 1 cut(s) 1886
CciI TCATGA 1 cut(s) 1489
CfoI GCGC 4 cut(s) 136, 253, 2932, 3070
Cfr10I RCCGGY 1 cut(s) 718
Cfr13I GGNCC 6 cut(s) 707, 1044, 1322, 2113, 2181, 2199
ClaI ATCGAT 1 cut(s) 1229
CseI GACGC 3 cut(s) 1364, 2861, 3055
CspCI CAANNNNNGTGG 4 cut(s) 2895, 2930, 3356, 3391
DdeI CTNAG 9 cut(s) 408, 901, 1381, 1887, 1989, 2524, 3167, 3324, 3411
DinI GGCGCC 1 cut(s) 2931
DraI TTTAAA 1 cut(s) 849
DraIII CACNNNGTG 1 cut(s) 704
EaeI YGGCCR 1 cut(s) 3018
Eam1104I CTCTTC 5 cut(s) 48, 657, 1038, 1398, 1911
EarI CTCTTC 5 cut(s) 48, 657, 1038, 1398, 1911
EciI GGCGGA 2 cut(s) 705, 2294
Eco130I CCWWGG 3 cut(s) 183, 273, 566
Eco24I GRGCYC 2 cut(s) 631, 1601
Eco32I GATATC 1 cut(s) 1233
Eco47I GGWCC 4 cut(s) 707, 1044, 1322, 2113
Eco57I CTGAAG 2 cut(s) 355, 2894
Eco88I CYCGRG 1 cut(s) 245
EcoO109I RGGNCCY 1 cut(s) 1322
EcoRII CCWGG 4 cut(s) 992, 1601, 1621, 2220
EcoRV GATATC 1 cut(s) 1233
EcoT14I CCWWGG 3 cut(s) 183, 273, 566
EcoT22I ATGCAT 2 cut(s) 1201, 2555
EcoT38I GRGCYC 2 cut(s) 631, 1601
EgeI GGCGCC 1 cut(s) 2931
EheI GGCGCC 1 cut(s) 2931
ErhI CCWWGG 3 cut(s) 183, 273, 566
FalI AAGNNNNNCTT 2 cut(s) 203, 235
FaqI GGGAC 1 cut(s) 1696
FauI CCCGC 2 cut(s) 133, 3240
FbaI TGATCA 1 cut(s) 2965
FblI GTMKAC 1 cut(s) 2985
FokI GGATG 7 cut(s) 164, 622, 772, 840, 1150, 2023, 2673
FriOI GRGCYC 2 cut(s) 631, 1601
FspBI CTAG 9 cut(s) 417, 431, 692, 1001, 1151, 1686, 1875, 3002, 3107
GlaI GCGC 4 cut(s) 135, 252, 2931, 3069
GsaI CCCAGC 1 cut(s) 985
GsuI CTGGAG 4 cut(s) 68, 104, 1585, 3079
HaeII RGCGCY 3 cut(s) 137, 254, 2933
HaeIII GGCC 6 cut(s) 571, 2183, 2201, 2459, 2784, 3020
HapII CCGG 2 cut(s) 719, 2294
HgaI GACGC 3 cut(s) 1364, 2861, 3055
HhaI GCGC 4 cut(s) 136, 253, 2932, 3070
Hin1I GRCGYC 2 cut(s) 2930, 3047
Hin6I GCGC 4 cut(s) 134, 251, 2930, 3068
HinP1I GCGC 4 cut(s) 134, 251, 2930, 3068
HincII GTYRAC 3 cut(s) 1609, 1960, 3104
HindII GTYRAC 3 cut(s) 1609, 1960, 3104
HindIII AAGCTT 3 cut(s) 479, 1267, 1575
HpaII CCGG 2 cut(s) 719, 2294
Hpy99I CGWCG 1 cut(s) 2855
HpyCH4III ACNGT 9 cut(s) 867, 1021, 1051, 1364, 1652, 1957, 2117, 2498, 3226
HpyCH4IV ACGT 3 cut(s) 317, 1421, 2266
HpyF3I CTNAG 9 cut(s) 408, 901, 1381, 1887, 1989, 2524, 3167, 3324, 3411
HpySE526I ACGT 3 cut(s) 317, 1421, 2266
Hsp92I GRCGYC 2 cut(s) 2930, 3047
HspAI GCGC 4 cut(s) 134, 251, 2930, 3068
KasI GGCGCC 1 cut(s) 2929
KroI GCCGGC 1 cut(s) 718
KroNI GCCGGC 1 cut(s) 720
Ksp22I TGATCA 1 cut(s) 2965
LmnI GCTCC 9 cut(s) 85, 691, 695, 1604, 1624, 2162, 2427, 3259, 3362
Lsp1109I GCAGC 9 cut(s) 556, 674, 712, 718, 734, 740, 746, 1933, 3095
LweI GCATC 9 cut(s) 358, 644, 862, 1128, 1606, 2045, 3004, 3139, 3161
MaeI CTAG 9 cut(s) 417, 431, 692, 1001, 1151, 1686, 1875, 3002, 3107
MaeII ACGT 3 cut(s) 317, 1421, 2266
MaeIII GTNAC 4 cut(s) 646, 996, 2939, 3395
MfeI CAATTG 1 cut(s) 2013
MflI RGATCY 3 cut(s) 2000, 2332, 3293
MhlI GDGCHC 6 cut(s) 631, 700, 1601, 1637, 2047, 3367
Mly113I GGCGCC 1 cut(s) 2930
MlyI GAGTC 6 cut(s) 77, 352, 1897, 2140, 2195, 2989
MmeI TCCRAC 3 cut(s) 641, 3006, 3259
Mph1103I ATGCAT 2 cut(s) 1201, 2555
MroNI GCCGGC 1 cut(s) 718
MroXI GAANNNNTTC 4 cut(s) 119, 215, 308, 2544
MslI CAYNNNNRTG 3 cut(s) 1640, 2948, 3188
MspA1I CMGCKG 5 cut(s) 553, 725, 731, 797, 3235
MspCI CTTAAG 1 cut(s) 2005
MspI CCGG 2 cut(s) 719, 2294
MspR9I CCNGG 4 cut(s) 994, 1603, 1623, 2222
MunI CAATTG 1 cut(s) 2013
Mva1269I GAATGC 2 cut(s) 2382, 3033
MvaI CCWGG 4 cut(s) 994, 1603, 1623, 2222
NaeI GCCGGC 1 cut(s) 720
NarI GGCGCC 1 cut(s) 2930
NgoMIV GCCGGC 1 cut(s) 718
NmeAIII GCCGAG 1 cut(s) 2209
NmuCI GTSAC 2 cut(s) 996, 2939
NsiI ATGCAT 2 cut(s) 1201, 2555
NspI RCATGY 3 cut(s) 989, 1029, 2951
NspV TTCGAA 1 cut(s) 229
OliI CACNNNNGTG 1 cut(s) 2948
PaeI GCATGC 2 cut(s) 989, 1029
PaeR7I CTCGAG 1 cut(s) 245
PagI TCATGA 1 cut(s) 1489
PcsI WCGNNNNNNNCGW 1 cut(s) 614
PctI GAATGC 2 cut(s) 2382, 3033
PdiI GCCGGC 1 cut(s) 720
PdmI GAANNNNTTC 4 cut(s) 119, 215, 308, 2544
PleI GAGTC 6 cut(s) 76, 352, 1897, 2140, 2194, 2989
PluTI GGCGCC 1 cut(s) 2933
PpsI GAGTC 6 cut(s) 76, 352, 1897, 2140, 2194, 2989
PpuMI RGGWCCY 1 cut(s) 1322
PshBI ATTAAT 2 cut(s) 525, 2103
PsiI TTATAA 1 cut(s) 1418
Psp1406I AACGTT 2 cut(s) 317, 2266
Psp5II RGGWCCY 1 cut(s) 1322
Psp6I CCWGG 4 cut(s) 992, 1601, 1621, 2220
PspFI CCCAGC 1 cut(s) 981
PspGI CCWGG 4 cut(s) 992, 1601, 1621, 2220
PspPI GGNCC 6 cut(s) 707, 1044, 1322, 2113, 2181, 2199
PspPPI RGGWCCY 1 cut(s) 1322
PstI CTGCAG 3 cut(s) 730, 736, 3258
PstNI CAGNNNCTG 1 cut(s) 1886
PsuI RGATCY 3 cut(s) 2000, 2332, 3293
PvuII CAGCTG 4 cut(s) 553, 725, 731, 797
RseI CAYNNNNRTG 3 cut(s) 1640, 2948, 3188
Sau96I GGNCC 6 cut(s) 707, 1044, 1322, 2113, 2181, 2199
ScaI AGTACT 1 cut(s) 119
SchI GAGTC 6 cut(s) 77, 352, 1897, 2140, 2195, 2989
ScrFI CCNGG 4 cut(s) 994, 1603, 1623, 2222
SduI GDGCHC 6 cut(s) 631, 700, 1601, 1637, 2047, 3367
SfaNI GCATC 9 cut(s) 358, 644, 862, 1128, 1606, 2045, 3004, 3139, 3161
SfcI CTRYAG 6 cut(s) 726, 732, 1020, 1102, 1648, 3254
SfoI GGCGCC 1 cut(s) 2931
Sfr274I CTCGAG 1 cut(s) 245
SfuI TTCGAA 1 cut(s) 229
SinI GGWCC 4 cut(s) 707, 1044, 1322, 2113
SlaI CTCGAG 1 cut(s) 245
SmiMI CAYNNNNRTG 3 cut(s) 1640, 2948, 3188
SmlI CTYRAG 2 cut(s) 245, 2005
SmoI CTYRAG 2 cut(s) 245, 2005
SpeI ACTAGT 3 cut(s) 1685, 1874, 3106
SphI GCATGC 2 cut(s) 989, 1029
SsiI CCGC 9 cut(s) 140, 716, 807, 2279, 2431, 2543, 3017, 3116, 3233
SspDI GGCGCC 1 cut(s) 2929
SspI AATATT 2 cut(s) 1534, 3136
SspMI CTAG 9 cut(s) 417, 431, 692, 1001, 1151, 1686, 1875, 3002, 3107
StyD4I CCNGG 4 cut(s) 992, 1601, 1621, 2220
StyI CCWWGG 3 cut(s) 183, 273, 566
TaaI ACNGT 9 cut(s) 867, 1021, 1051, 1364, 1652, 1957, 2117, 2498, 3226
TaiI ACGT 3 cut(s) 320, 1424, 2269
TaqII GACCGA 1 cut(s) 2158
TatI WGTACW 6 cut(s) 117, 333, 1859, 1877, 3058, 3192
TauI GCSGC 4 cut(s) 809, 2433, 3020, 3119
TscAI CASTG 7 cut(s) 1054, 1369, 1634, 1657, 2306, 2503, 3047
TseFI GTSAC 2 cut(s) 996, 2939
TseI GCWGC 9 cut(s) 544, 662, 722, 725, 728, 731, 734, 1946, 3083
Tsp45I GTSAC 2 cut(s) 996, 2939
TspGWI ACGGA 6 cut(s) 61, 461, 1674, 1770, 1980, 3375
TspRI CASTG 7 cut(s) 1054, 1369, 1634, 1657, 2306, 2503, 3047
Vha464I CTTAAG 1 cut(s) 2005
VneI GTGCAC 1 cut(s) 1633
VpaK11BI GGWCC 4 cut(s) 707, 1044, 1322, 2113
VspI ATTAAT 2 cut(s) 525, 2103
XapI RAATTY 4 cut(s) 59, 745, 1936, 2368
XceI RCATGY 3 cut(s) 989, 1029, 2951
XcmI CCANNNNNNNNNTGG 3 cut(s) 1170, 2107, 3275
XhoI CTCGAG 1 cut(s) 245
XmiI GTMKAC 1 cut(s) 2985
XmnI GAANNNNTTC 4 cut(s) 119, 215, 308, 2544
XspI CTAG 9 cut(s) 417, 431, 692, 1001, 1151, 1686, 1875, 3002, 3107
ZrmI AGTACT 1 cut(s) 119
Zsp2I ATGCAT 2 cut(s) 1201, 2555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.