MD13G1162100.v1.1

Topless-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0013792
Physical Location & Seq
Reverse (-)
12757419 .. 12764824
7406 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1162100.v1.1.491

Sequence Viewer

Length: 3420 bp
ATGTCGTCCTTGAGCAGAGAACTGGTGTTCCTGATCCTCCAATTTCTGGAGGAAGAGAAGTTCAAGGAGTCCGTACACAGGCTGGAGCAGGAATCGGGGTACTTCTTCAACATGAAGTACTTTGAGGAGAAGGCGCTGGCTGGAGAGTGGGACGAGGTCGAGAAGTACCTCTCTGGGTTCACCAAGGTCGATGAGAATCGCTACTCGATGAAGATTTTCTTCGAGGTTCGAAAGCAGAAGTATCTCGAGGCGCTCGACAGGAATGACAGAGCTAAGGCTGTTGAGATACTGGTGAAGGATTTGAAAGTGTTCTCGACGTTTAACGAAGATTTGTACAAGGAAATTACGCATCTTTTGACCCTCGAAAACTTTAGAGAAAATGAGCAGTTATCGAAATATGGTGATACTAAGTCGGCTAGAAGCATAATGCTAGTAGAGCTTAAAAAGTTGATAGAAGCAAATCCGCTTTTCCGAGATAAGCTTGCTTTACCCACTTTGAAGGCTTCACGCTTGCGAACTCTAATTAATCAAAGTCTAAATTGGCAGCACCAGCTGTGCAAGAACCCAAGGCCAAATCCTGATATTAAGACCTTATTCATGGACCACTCGTGTTCTCCAACAAATGGGGCTCGTGCATCCACGCCAGTTACTCTTCCAGTTGCAGCTCTTGCAAAGCCAACTTATGCTCCTCTAGGAGCACATGGTGGACCTTTTCCGCCGGCTGCTGCAGCTGCAGCTAATGCAAATTTAGCAGGATGGATGTCGAATGCTAATCCTTCCATGTCTGTGCAATCAGCTGTTGTTGCCGCTTCACCCTTCCCTGTTCAACCCAGTCAAGGTTCTGGTTTAAAGCATCCAAGAACACCCTCAAATGCTCTTGGAATGAGCGAGTATCAAGGCTCAGATCATGAGCAACTAATGAAACGCCTGCGGCCTGCACAATCTGTTGATGAGGTCAGTTATCCTCCCCCTACCCAGCATGCTTCCTGGTCACTAGATGACCTACCGAGGACCGTAGCGTGCACTCTTCGTCAGGGATTCAATGTGATAAGCATGGATTTCCACCCTTCTCATCACACATTACTTGCTGTTGGATGTAGTAATGGTGAAATTACACTGTGGGAAGTTGGGATACGAGAGAAGCTAGTGACAAAGCCATTCAAAATATGGAATATGACTCCTAGTTCTAATGCAATTCAGCTTGCTATGGTCAAAGATTCGTCAATGTCTGTAAGCCGTGTATCATGGAATCAAGATGGAAGCTTTATTGGTGTTGCATTTACCAAACACTTGGTTCATTTGTACGCTTATCAAGGACCAACTGATCTACGGCAACATTTGGAGATTGATGCTCACAGTGGCAGTGTGAATGACTTAGCCTTTTCTCATCCAAACAAGCAATTATGTCTTATAACTTGTGGGGAGGATAAGCTGATAAAGGTTTGGGATTTGGCTGGAAGAAATCTATTCAGCTTTGAAGGTCATGAAGCGCCTGTTTACTCAATTTGCCCCCACCAGAAGGAGAATATTCAGTTCATATTTTCAACTGCTGTTGATGGGAAAATAAAAGCTTGGCTGTATGACAATGTGGGCTCCAGGGTTGACTATGATGCTCCTGGGCAGTGGTGCACCACAATGCTCTACAGTGATGATGGAAATAGATTATTCTCTTGTGGGACTAGTAAAGATGGTGATTCTTTCCTCGTTGAATGGAATGAAAGTGAAGGGGCAATAAAGAGGACATATACTGGATTCAGAAAAAAATCTTCTGGCGTAGTGCAATTTGATACAACAAAAAATCACTTTTTGGCTGTTGGTGAAGATAATCAGATAAAGTTTTGGGATATGGATAATACAAATGTTCTTACTGGTACAGAAGCTGAGGGTGGACTTCTGACTCTTCCCCGCTTGAGATTCAATAAGGAAGGAAATTTGCTTGCTGCTACCACGTCTGACAACGGAATCAAAATTCTTGCTAATGCTGAGGGTCTAAGATCCTTAAGAGCAATTGAAACTCGATCTTATGAAGCATCCAGAGGACCTATTGAGATGAAGGTATCTGGTTCTTCCATGGTTCCGAATGTCAACCCAACCATTAATAAAGTGGACCGTTTGGATACAAGCTCTCCTGCCAGACCGACTCATATTCTTAATGGAGCTGATTCTATGGCAAGAGCTGATTCTATGGCAAGAGCCGATTCTATGGCAAGAGCCGATATGGCAAGAAGCCTGGAAAAGAGAAGCTTAGATGATGTATCTGACAAGATTAAACGTTGGGAACTGGCGGAAATTGTAGATCCCGTTCAGTGTCGAGTGGCTACCATGCCAGAAAGTAAAGATCCTGCTAACAAGGTTGCTCGACTTCTGTACACAAATTCTGGTTCTGGCATTCTGGCTCTCGGTTCAAATGGGGTGCAGAAGCTATGGAAGTGGAGCCGCAATGAGCAAAATCCAAGTGGGAAGGCCACAGCAAGTGTTGTTCCCCAACATTGGCAACCCAACAGTGGTCTTTTAATGACTAATGATGTCTCAGAAAATGTTGAAGAAGCGGTTCCATGCATTGCACTCTCAAAAAATGACTCTTATGTGATGTCAGCCTGTGGAGGAAAAGTGTCTCTGTTCAATATGATGACTTTTAAGGTAATGACAACTTTCATGCCACCTCCACCAGTGTCAACCTACTTGTCATTTCATCCTCTGGATAATAATATCATAGCAATAGGAATGGAAGATTCAACTATCCACATTTACAATGTTCGACTTGATGAGGTCAAAGCAAAACTGAAAGGCCACCAGAAGCACATTACTGGTTTAGCATTTTCTGTCAACCTTAAAATCATGGTTTCATCAGGTGCTGATGCTCAACTATGCTTTTGGAACATGGATACATGGGATAAGAGGAAATCGGTTCCACTTCAGTTGCCCGCTGGAAAGGCGCCTGTCGGTGACACGCATGTGCAATTCTATTCTGATCAAGTCCGATTGTTGGTATACCATGAGACTCAACTAGCATTATATGATGCGGCCAAATCAGAGTGCATTCGGCAGTGGACGCCACAAGATCTTCTGCCTGCGCCCATTTCTTGTGCAGCATATTCTTCAAGTAGTCAACTAGTTTATGCGGCATTTACTGATGGTAACATTGGAGTATTTGATGCTGATAGTCTGAAACTAAGATGCCGTATTGCCATGTCGGTGTACTTACCACAAGCATCATCAAACAGCCCATCAGTGTACCCGCTGGCTCTTACAGCACATTTGCAGGAGCCGTACCAATTTGCTGTTGGACTGACAGATGGATCTGTTAAAGTTATAGAGCCCTCGGAATCTGAGGGGAAGTGGGGAGTTTTGGTGCCCGTTGATAACGGAACACAGAACGGGTGGACAGCAACATCTTCGAGTAACAATCCAGCATCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

1140

Amino Acids

126.09

Weight (kDa)

6.5

Isoelectric Point (pI)

43.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH_TPL PF17814 5 - 33 3.5e-11 LisH-like dimerisation domain
TPR1-like_2nd PF21889 37 - 161 1.1e-61 TPR1-like, CTLH-containing domain
zf_topless PF21359 164 - 204 2.9e-25 TOPLESS, zinc finger domain
Beta-prop_EML_2 PF23414 330 - 466 8.2e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 345 - 529 4e-08 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd PF25172 348 - 562 4.7e-09 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 388 - 525 4.2e-13 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 389 - 485 2.5e-07 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 406 - 622 5.7e-19 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 409 - 663 2.1e-22 THOC3 beta-propeller domain
Beta-prop_EML PF23409 447 - 650 3.3e-06 Echinoderm microtubule-associated protein first beta-propeller
WD40 PF00400 448 - 483 6.7e-06 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 453 - 528 1.6e-07 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 463 - 596 2.2e-06 Echinoderm microtubule-associated protein second beta-propeller
WDR55 PF24796 496 - 656 8.9e-07 WDR55
Beta-prop_WDR3_1st PF25173 519 - 657 1.9e-06 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 831 - 1040 2.8e-14 Prp19 WD40 domain
WDR55 PF24796 842 - 962 2.7e-09 WDR55
WD40_WDHD1_1st PF24817 842 - 1057 5.2e-15 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 843 - 967 3.5e-11 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 844 - 966 1.2e-14 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 845 - 968 7.8e-12 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 848 - 1055 1.9e-16 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 848 - 963 2e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 851 - 969 3.4e-12 WDR3 second beta-propeller domain
Beta-prop_IFT140_1st PF23383 864 - 968 6.7e-09 IFT140 first beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 868 - 963 2.1e-10 WDR36/Utp21 first beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 870 - 962 8.7e-08 WDR90/POC16, second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013792)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G16830
fragaria_vesca FvH4_4g18910 FvH4_4g18910
malus_domestica MD13G1162100.v1.1 MD16G1161900.v1.1
prunus_persica Prupe.1G129400_v2.0.a1
pyrus_communis pycom13g13830
rosa_chinensis RchiOBHm_Chr4g0423811
rosa_laevigata RLG00000007530
rosa_multiflora Rmu_sc0003369.1_g000018
rosa_roxburghii Rroxscaffold_5G00365920
rosa_rugosa Rorug04G0190900
rosa_samantha Rh4AG250300 Rh4BG255600 Rh4CG263700 Rh4DG250000
rosa_wichuraiana Rw4G021430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1412
AccB1I GGYRCC 2 cut(s) 2935, 3352
AccB7I CCANNNNNTGG 2 cut(s) 46, 623
AccI GTMKAC 1 cut(s) 2991
AclI AACGTT 1 cut(s) 2272
AclWI GGATC 5 cut(s) 28, 1989, 2291, 2333, 3307
AcoI YGGCCR 1 cut(s) 3024
AcsI RAATTY 4 cut(s) 745, 1930, 1968, 2374
AcuI CTGAAG 1 cut(s) 2900
AcyI GRCGYC 2 cut(s) 2936, 3053
AdeI CACNNNGTG 1 cut(s) 704
AfiI CCNNNNNNNGG 8 cut(s) 46, 78, 623, 836, 1519, 2490, 2504, 2986
AflII CTTAAG 1 cut(s) 1999
AhlI ACTAGT 2 cut(s) 1679, 3112
AjiI CACGTC 1 cut(s) 1950
AjnI CCWGG 4 cut(s) 986, 1595, 1615, 2229
AleI CACNNNNGTG 1 cut(s) 2954
AloI GAACNNNNNNTCC 4 cut(s) 12, 44, 44, 76
Alw21I GWGCWC 3 cut(s) 700, 1025, 1631
Alw26I GTCTC 3 cut(s) 2533, 2619, 2993
Alw44I GTGCAC 2 cut(s) 1021, 1627
AlwI GGATC 5 cut(s) 28, 1989, 2291, 2333, 3307
AlwNI CAGNNNCTG 2 cut(s) 1880, 2855
Ama87I CYCGRG 1 cut(s) 245
AoxI GGCC 5 cut(s) 569, 932, 2463, 2788, 3024
ApaLI GTGCAC 2 cut(s) 1021, 1627
ApeKI GCWGC 9 cut(s) 544, 662, 722, 725, 728, 731, 734, 1940, 3089
ApoI RAATTY 4 cut(s) 745, 1930, 1968, 2374
AseI ATTAAT 2 cut(s) 525, 2097
Asp700I GAANNNNTTC 3 cut(s) 215, 308, 2550
AspLEI GCGC 5 cut(s) 136, 253, 1492, 2938, 3076
AspS9I GGNCC 6 cut(s) 601, 707, 1011, 1316, 2039, 2107
AsuHPI GGTGA 8 cut(s) 172, 304, 413, 804, 1118, 1703, 1829, 2957
AsuII TTCGAA 1 cut(s) 229
AvaI CYCGRG 1 cut(s) 245
AvaII GGWCC 6 cut(s) 601, 707, 1011, 1316, 2039, 2107
BaeGI GKGCMC 3 cut(s) 1025, 1631, 3357
BanI GGYRCC 2 cut(s) 2935, 3352
BanII GRGCYC 3 cut(s) 631, 1595, 3321
BarI GAAGNNNNNNTAC 2 cut(s) 1116, 1148
BauI CACGAG 2 cut(s) 607, 630
Bbv12I GWGCWC 3 cut(s) 700, 1025, 1631
BbvCI CCTCAGC 2 cut(s) 1881, 1983
BbvI GCAGC 9 cut(s) 556, 674, 709, 712, 718, 740, 746, 1927, 3101
BccI CCATC 8 cut(s) 750, 1250, 1550, 1646, 1682, 3128, 3235, 3290
BceAI ACGGC 4 cut(s) 1221, 1346, 3165, 3253
BcgI CGANNNNNNTGC 2 cut(s) 3378, 3412
BciT130I CCWGG 4 cut(s) 988, 1597, 1617, 2231
BciVI GTATCC 3 cut(s) 1125, 2110, 2878
BclI TGATCA 1 cut(s) 2971
BcoDI GTCTC 3 cut(s) 2533, 2619, 2993
BcuI ACTAGT 2 cut(s) 1679, 3112
BfaI CTAG 9 cut(s) 417, 431, 692, 995, 1145, 1182, 1680, 3008, 3113
BfmI CTRYAG 3 cut(s) 726, 732, 1642
BfoI RGCGCY 4 cut(s) 137, 254, 1493, 2939
BfrI CTTAAG 1 cut(s) 1999
BfuI GTATCC 3 cut(s) 1125, 2110, 2878
BglI GCCNNNNNGGC 1 cut(s) 2219
BglII AGATCT 1 cut(s) 3061
BmcAI AGTACT 1 cut(s) 119
Bme1390I CCNGG 4 cut(s) 988, 1597, 1617, 2231
Bme18I GGWCC 6 cut(s) 601, 707, 1011, 1316, 2039, 2107
BmeT110I CYCGRG 1 cut(s) 245
BmgBI CACGTC 1 cut(s) 1950
BmgT120I GGNCC 6 cut(s) 601, 707, 1011, 1316, 2039, 2107
BmiI GGNNCC 8 cut(s) 1594, 2076, 2435, 2553, 2910, 2937, 3267, 3354
BmrFI CCNGG 4 cut(s) 988, 1597, 1617, 2231
BmrI ACTGGG 1 cut(s) 825
BmuI ACTGGG 1 cut(s) 825
BpmI CTGGAG 4 cut(s) 68, 104, 162, 1579
Bpu10I CCTNAGC 3 cut(s) 273, 1881, 1983
Bpu14I TTCGAA 1 cut(s) 229
BpuEI CTTGAG 2 cut(s) 31, 1930
BsaBI GATNNNNATC 1 cut(s) 195
BsaHI GRCGYC 2 cut(s) 2936, 3053
BsaJI CCNNGG 7 cut(s) 183, 566, 1007, 1596, 1616, 2070, 3321
BsaXI ACNNNNNCTCC 8 cut(s) 670, 700, 2110, 2140, 2595, 2625, 3336, 3366
Bsc4I CCNNNNNNNGG 8 cut(s) 46, 78, 623, 836, 1519, 2490, 2504, 2986
Bse118I RCCGGY 1 cut(s) 718
Bse3DI GCAATG 2 cut(s) 2446, 2559
Bse8I GATNNNNATC 1 cut(s) 195
BseBI CCWGG 4 cut(s) 988, 1597, 1617, 2231
BseDI CCNNGG 7 cut(s) 183, 566, 1007, 1596, 1616, 2070, 3321
BseGI GGATG 8 cut(s) 635, 761, 765, 853, 1100, 1387, 2030, 2692
BseJI GATNNNNATC 1 cut(s) 195
BseLI CCNNNNNNNGG 8 cut(s) 46, 78, 623, 836, 1519, 2490, 2504, 2986
BseMI GCAATG 2 cut(s) 2446, 2559
BseMII CTCAG 5 cut(s) 915, 1872, 1974, 2544, 3321
BseRI GAGGAG 2 cut(s) 140, 678
BseSI GKGCMC 3 cut(s) 1025, 1631, 3357
BseXI GCAGC 9 cut(s) 556, 674, 709, 712, 718, 740, 746, 1927, 3101
BseYI CCCAGC 1 cut(s) 975
BsgI GTGCAG 3 cut(s) 921, 2435, 3108
BshFI GGCC 5 cut(s) 571, 934, 2465, 2790, 3026
BshNI GGYRCC 2 cut(s) 2935, 3352
BsiHKAI GWGCWC 3 cut(s) 700, 1025, 1631
BsiHKCI CYCGRG 1 cut(s) 245
BsiSI CCGG 1 cut(s) 719
BslFI GGGAC 2 cut(s) 164, 1690
BslI CCNNNNNNNGG 8 cut(s) 46, 78, 623, 836, 1519, 2490, 2504, 2986
BsmAI GTCTC 3 cut(s) 2533, 2619, 2993
BsmFI GGGAC 2 cut(s) 164, 1690
BsmI GAATGC 3 cut(s) 772, 2388, 3039
BsnI GGCC 5 cut(s) 571, 934, 2465, 2790, 3026
BsoBI CYCGRG 1 cut(s) 245
Bsp119I TTCGAA 1 cut(s) 229
Bsp1286I GDGCHC 7 cut(s) 631, 700, 1025, 1595, 1631, 3321, 3357
Bsp1407I TGTACA 2 cut(s) 333, 2367
Bsp19I CCATGG 1 cut(s) 2070
BspANI GGCC 5 cut(s) 571, 934, 2465, 2790, 3026
BspCNI CTCAG 5 cut(s) 914, 1873, 1975, 2543, 3322
BspHI TCATGA 2 cut(s) 907, 1483
BspLI GGNNCC 8 cut(s) 1594, 2076, 2435, 2553, 2910, 2937, 3267, 3354
BspMAI CTGCAG 2 cut(s) 730, 736
BspPI GGATC 5 cut(s) 28, 1989, 2291, 2333, 3307
BspT104I TTCGAA 1 cut(s) 229
BspT107I GGYRCC 2 cut(s) 2935, 3352
BspTI CTTAAG 1 cut(s) 1999
BsrDI GCAATG 2 cut(s) 2446, 2559
BsrFI RCCGGY 1 cut(s) 718
BsrGI TGTACA 2 cut(s) 333, 2367
BssAI RCCGGY 1 cut(s) 718
BssECI CCNNGG 7 cut(s) 183, 566, 1007, 1596, 1616, 2070, 3321
BssNAI GTATAC 1 cut(s) 2992
BssNI GRCGYC 2 cut(s) 2936, 3053
BssSI CACGAG 2 cut(s) 607, 630
BssT1I CCWWGG 3 cut(s) 183, 566, 2070
Bst1107I GTATAC 1 cut(s) 2992
Bst2BI CACGAG 2 cut(s) 607, 630
Bst2UI CCWGG 4 cut(s) 988, 1597, 1617, 2231
Bst4CI ACNGT 6 cut(s) 1015, 1119, 1358, 1646, 2111, 2504
Bst6I CTCTTC 4 cut(s) 48, 657, 1032, 1905
BstACI GRCGYC 2 cut(s) 2936, 3053
BstAFI CTTAAG 1 cut(s) 1999
BstAPI GCANNNNNTGC 2 cut(s) 668, 740
BstAUI TGTACA 2 cut(s) 333, 2367
BstBI TTCGAA 1 cut(s) 229
BstDSI CCRYGG 1 cut(s) 2070
BstF5I GGATG 8 cut(s) 635, 761, 765, 853, 1100, 1387, 2030, 2692
BstH2I RGCGCY 4 cut(s) 137, 254, 1493, 2939
BstHHI GCGC 5 cut(s) 136, 253, 1492, 2938, 3076
BstMAI GTCTC 3 cut(s) 2533, 2619, 2993
BstNI CCWGG 4 cut(s) 988, 1597, 1617, 2231
BstNSI RCATGY 2 cut(s) 983, 2957
BstSCI CCNGG 4 cut(s) 986, 1595, 1615, 2229
BstSFI CTRYAG 3 cut(s) 726, 732, 1642
BstSLI GKGCMC 3 cut(s) 1025, 1631, 3357
BstV1I GCAGC 9 cut(s) 556, 674, 709, 712, 718, 740, 746, 1927, 3101
BstX2I RGATCY 5 cut(s) 1994, 2296, 2338, 3061, 3299
BstXI CCANNNNNNTGG 1 cut(s) 1291
BstYI RGATCY 5 cut(s) 1994, 2296, 2338, 3061, 3299
BstZ17I GTATAC 1 cut(s) 2992
BsuI GTATCC 3 cut(s) 1125, 2110, 2878
BsuRI GGCC 5 cut(s) 571, 934, 2465, 2790, 3026
BtgI CCRYGG 1 cut(s) 2070
BtrI CACGTC 1 cut(s) 1950
BtsCI GGATG 8 cut(s) 635, 761, 765, 853, 1100, 1387, 2030, 2692
BtsI GCAGTG 3 cut(s) 1369, 1628, 3053
CaiI CAGNNNCTG 2 cut(s) 1880, 2855
CciI TCATGA 2 cut(s) 907, 1483
CfoI GCGC 5 cut(s) 136, 253, 1492, 2938, 3076
Cfr10I RCCGGY 1 cut(s) 718
Cfr13I GGNCC 6 cut(s) 601, 707, 1011, 1316, 2039, 2107
CseI GACGC 1 cut(s) 3061
CspCI CAANNNNNGTGG 2 cut(s) 2901, 2936
DinI GGCGCC 1 cut(s) 2937
DraI TTTAAA 1 cut(s) 849
DraIII CACNNNGTG 1 cut(s) 704
EaeI YGGCCR 1 cut(s) 3024
Eam1104I CTCTTC 4 cut(s) 48, 657, 1032, 1905
EarI CTCTTC 4 cut(s) 48, 657, 1032, 1905
EciI GGCGGA 2 cut(s) 705, 2300
Eco130I CCWWGG 3 cut(s) 183, 566, 2070
Eco24I GRGCYC 3 cut(s) 631, 1595, 3321
Eco47I GGWCC 6 cut(s) 601, 707, 1011, 1316, 2039, 2107
Eco57I CTGAAG 1 cut(s) 2900
Eco88I CYCGRG 1 cut(s) 245
EcoO109I RGGNCCY 1 cut(s) 2039
EcoRII CCWGG 4 cut(s) 986, 1595, 1615, 2229
EcoT14I CCWWGG 3 cut(s) 183, 566, 2070
EcoT22I ATGCAT 1 cut(s) 2561
EcoT38I GRGCYC 3 cut(s) 631, 1595, 3321
EgeI GGCGCC 1 cut(s) 2937
EheI GGCGCC 1 cut(s) 2937
ErhI CCWWGG 3 cut(s) 183, 566, 2070
FalI AAGNNNNNCTT 4 cut(s) 203, 235, 2228, 2260
FaqI GGGAC 2 cut(s) 164, 1690
FauI CCCGC 3 cut(s) 1913, 2932, 3246
FbaI TGATCA 1 cut(s) 2971
FblI GTMKAC 1 cut(s) 2991
FokI GGATG 8 cut(s) 622, 768, 772, 840, 1107, 1374, 2017, 2679
FriOI GRGCYC 3 cut(s) 631, 1595, 3321
FspBI CTAG 9 cut(s) 417, 431, 692, 995, 1145, 1182, 1680, 3008, 3113
GlaI GCGC 5 cut(s) 135, 252, 1491, 2937, 3075
GsaI CCCAGC 1 cut(s) 979
GsuI CTGGAG 4 cut(s) 68, 104, 162, 1579
HaeII RGCGCY 4 cut(s) 137, 254, 1493, 2939
HaeIII GGCC 5 cut(s) 571, 934, 2465, 2790, 3026
HapII CCGG 1 cut(s) 719
HgaI GACGC 1 cut(s) 3061
HhaI GCGC 5 cut(s) 136, 253, 1492, 2938, 3076
Hin1I GRCGYC 2 cut(s) 2936, 3053
Hin6I GCGC 5 cut(s) 134, 251, 1490, 2936, 3074
HinP1I GCGC 5 cut(s) 134, 251, 1490, 2936, 3074
HincII GTYRAC 5 cut(s) 1603, 2086, 2676, 2827, 3110
HindII GTYRAC 5 cut(s) 1603, 2086, 2676, 2827, 3110
HindIII AAGCTT 4 cut(s) 479, 1261, 1569, 2242
HpaII CCGG 1 cut(s) 719
HphI GGTGA 8 cut(s) 172, 304, 413, 804, 1118, 1703, 1829, 2957
Hpy99I CGWCG 1 cut(s) 319
HpyCH4III ACNGT 6 cut(s) 1015, 1119, 1358, 1646, 2111, 2504
HpyCH4IV ACGT 3 cut(s) 317, 1949, 2272
HpySE526I ACGT 3 cut(s) 317, 1949, 2272
Hsp92I GRCGYC 2 cut(s) 2936, 3053
HspAI GCGC 5 cut(s) 134, 251, 1490, 2936, 3074
KasI GGCGCC 1 cut(s) 2935
KroI GCCGGC 1 cut(s) 718
KroNI GCCGGC 1 cut(s) 720
Ksp22I TGATCA 1 cut(s) 2971
LmnI GCTCC 8 cut(s) 85, 691, 695, 1598, 1618, 2156, 2433, 3265
Lsp1109I GCAGC 9 cut(s) 556, 674, 709, 712, 718, 740, 746, 1927, 3101
MaeI CTAG 9 cut(s) 417, 431, 692, 995, 1145, 1182, 1680, 3008, 3113
MaeII ACGT 3 cut(s) 317, 1949, 2272
MaeIII GTNAC 6 cut(s) 646, 990, 1147, 2945, 3137, 3401
MfeI CAATTG 1 cut(s) 2007
MflI RGATCY 5 cut(s) 1994, 2296, 2338, 3061, 3299
MhlI GDGCHC 7 cut(s) 631, 700, 1025, 1595, 1631, 3321, 3357
Mly113I GGCGCC 1 cut(s) 2936
MlyI GAGTC 6 cut(s) 77, 1171, 1891, 2134, 2573, 2995
MmeI TCCRAC 3 cut(s) 641, 1072, 3265
Mph1103I ATGCAT 1 cut(s) 2561
MroNI GCCGGC 1 cut(s) 718
MroXI GAANNNNTTC 3 cut(s) 215, 308, 2550
MslI CAYNNNNRTG 4 cut(s) 785, 1634, 2954, 3194
MspA1I CMGCKG 5 cut(s) 553, 731, 797, 2927, 3241
MspCI CTTAAG 1 cut(s) 1999
MspI CCGG 1 cut(s) 719
MspR9I CCNGG 4 cut(s) 988, 1597, 1617, 2231
MunI CAATTG 1 cut(s) 2007
Mva1269I GAATGC 3 cut(s) 772, 2388, 3039
MvaI CCWGG 4 cut(s) 988, 1597, 1617, 2231
NaeI GCCGGC 1 cut(s) 720
NarI GGCGCC 1 cut(s) 2936
NcoI CCATGG 1 cut(s) 2070
NgoMIV GCCGGC 1 cut(s) 718
NlaIV GGNNCC 8 cut(s) 1594, 2076, 2435, 2553, 2910, 2937, 3267, 3354
NmuCI GTSAC 3 cut(s) 990, 1147, 2945
NsiI ATGCAT 1 cut(s) 2561
NspI RCATGY 2 cut(s) 983, 2957
NspV TTCGAA 1 cut(s) 229
OliI CACNNNNGTG 1 cut(s) 2954
PaeI GCATGC 1 cut(s) 983
PaeR7I CTCGAG 1 cut(s) 245
PagI TCATGA 2 cut(s) 907, 1483
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PctI GAATGC 3 cut(s) 772, 2388, 3039
PdiI GCCGGC 1 cut(s) 720
PdmI GAANNNNTTC 3 cut(s) 215, 308, 2550
PflFI GACNNNGTC 1 cut(s) 155
PflMI CCANNNNNTGG 2 cut(s) 46, 623
PleI GAGTC 6 cut(s) 76, 1171, 1891, 2134, 2573, 2995
PluTI GGCGCC 1 cut(s) 2939
PpsI GAGTC 6 cut(s) 76, 1171, 1891, 2134, 2573, 2995
PpuMI RGGWCCY 1 cut(s) 2039
PshBI ATTAAT 2 cut(s) 525, 2097
PsiI TTATAA 1 cut(s) 1412
Psp1406I AACGTT 1 cut(s) 2272
Psp5II RGGWCCY 1 cut(s) 2039
Psp6I CCWGG 4 cut(s) 986, 1595, 1615, 2229
PspFI CCCAGC 1 cut(s) 975
PspGI CCWGG 4 cut(s) 986, 1595, 1615, 2229
PspN4I GGNNCC 8 cut(s) 1594, 2076, 2435, 2553, 2910, 2937, 3267, 3354
PspPI GGNCC 6 cut(s) 601, 707, 1011, 1316, 2039, 2107
PspPPI RGGWCCY 1 cut(s) 2039
PsrI GAACNNNNNNTAC 2 cut(s) 2286, 2318
PstI CTGCAG 2 cut(s) 730, 736
PstNI CAGNNNCTG 2 cut(s) 1880, 2855
PsuI RGATCY 5 cut(s) 1994, 2296, 2338, 3061, 3299
PsyI GACNNNGTC 1 cut(s) 155
PvuII CAGCTG 3 cut(s) 553, 731, 797
RseI CAYNNNNRTG 4 cut(s) 785, 1634, 2954, 3194
Sau96I GGNCC 6 cut(s) 601, 707, 1011, 1316, 2039, 2107
ScaI AGTACT 1 cut(s) 119
SchI GAGTC 6 cut(s) 77, 1171, 1891, 2134, 2573, 2995
ScrFI CCNGG 4 cut(s) 988, 1597, 1617, 2231
SduI GDGCHC 7 cut(s) 631, 700, 1025, 1595, 1631, 3321, 3357
SfcI CTRYAG 3 cut(s) 726, 732, 1642
SfoI GGCGCC 1 cut(s) 2937
Sfr274I CTCGAG 1 cut(s) 245
SfuI TTCGAA 1 cut(s) 229
SinI GGWCC 6 cut(s) 601, 707, 1011, 1316, 2039, 2107
SlaI CTCGAG 1 cut(s) 245
SmiMI CAYNNNNRTG 4 cut(s) 785, 1634, 2954, 3194
SmlI CTYRAG 4 cut(s) 10, 245, 1909, 1999
SmoI CTYRAG 4 cut(s) 10, 245, 1909, 1999
SpeI ACTAGT 2 cut(s) 1679, 3112
SphI GCATGC 1 cut(s) 983
SspDI GGCGCC 1 cut(s) 2935
SspI AATATT 1 cut(s) 1528
SspMI CTAG 9 cut(s) 417, 431, 692, 995, 1145, 1182, 1680, 3008, 3113
StyD4I CCNGG 4 cut(s) 986, 1595, 1615, 2229
StyI CCWWGG 3 cut(s) 183, 566, 2070
TaaI ACNGT 6 cut(s) 1015, 1119, 1358, 1646, 2111, 2504
TaiI ACGT 3 cut(s) 320, 1952, 2275
TaqII GACCGA 1 cut(s) 2152
TatI WGTACW 4 cut(s) 117, 333, 2367, 3198
TauI GCSGC 5 cut(s) 809, 934, 2439, 3026, 3125
TseFI GTSAC 3 cut(s) 990, 1147, 2945
TseI GCWGC 9 cut(s) 544, 662, 722, 725, 728, 731, 734, 1940, 3089
Tsp45I GTSAC 3 cut(s) 990, 1147, 2945
TspGWI ACGGA 3 cut(s) 61, 1974, 3381
Tth111I GACNNNGTC 1 cut(s) 155
Van91I CCANNNNNTGG 2 cut(s) 46, 623
Vha464I CTTAAG 1 cut(s) 1999
VneI GTGCAC 2 cut(s) 1021, 1627
VpaK11BI GGWCC 6 cut(s) 601, 707, 1011, 1316, 2039, 2107
VspI ATTAAT 2 cut(s) 525, 2097
XapI RAATTY 4 cut(s) 745, 1930, 1968, 2374
XceI RCATGY 2 cut(s) 983, 2957
XcmI CCANNNNNNNNNTGG 3 cut(s) 1164, 2101, 3281
XhoI CTCGAG 1 cut(s) 245
XmiI GTMKAC 1 cut(s) 2991
XmnI GAANNNNTTC 3 cut(s) 215, 308, 2550
XspI CTAG 9 cut(s) 417, 431, 692, 995, 1145, 1182, 1680, 3008, 3113
ZrmI AGTACT 1 cut(s) 119
Zsp2I ATGCAT 1 cut(s) 2561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.