Rh4DG250000

Topless-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0013792
Physical Location & Seq
Forward (+)
46323236 .. 46330954
7719 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG250000.1

Sequence Viewer

Length: 2865 bp
ATGTCATCTCTGAGCAGGGAGCTGGTTTTCCTGATCCTTCAATTTCTTGAGGAGGAGAAATTCAAGGAGGCTGTACATAGGCTTGAGCAAGAGTCTGGATTCTTCTTCAACATGAAGTACTTCGAGGAGAAAGCGTTGCAGGGAGAGTGGGACGAGGTGGAGAAGTACCTGTCCGGGTTCACCAAGGTGGACGAGAATCGCTACTCTATGAAGATCTTCTTCGAGGTCAGAAAGCAGAAGTATCTCGAAGCGCTCGACAGGCAAGACAGGGCGAAAGCTGTTGAGATACTGGTGAAGGATTTGAAGGTGTTTTCGACGTTTAATGAGGACTTGTACAAGGAAATCACTCATCTGTTGACTCTTGACAACTTCAGAGAAAATGAGCAGTTGTCGAAGTATGGTGATACTAAATCCGCTAGAAGCATAATGCTGGTAGAGCTGAAGAAATTGATAGAAGCAAATCCTCTATTCCGAGAGAAGCTGGTTTTCCCCACTTTGAAGGCATCACGCTTGCGAACTTTGATCAATCAAAGTTTAAATTGGCAGCATCAGCTATGCAAAAACCCAAGGCCAAATCCTGACATTAAGACCTTATTCATGGACCACTCGTGTTCTCCTCCAAATGGAGCTCGTGCATCGACTCCAGTTACTCTTCCAGTTGCCGCTCTTACAAAGCCTTTAACCTATGCTCCTCTTGGAGCACATGGTGGACCTTTCCCGCCGGCTGCTGCTGCTGCTGCAGCTGCCAATGCGAATGCTTTAGCAGGATGGATGTCCAATGCTAATCCGTCCTTGTCTATGCAATCAGCTCCGGTCTCTGCTTCACCCCTGCCTGTTCAACTTGGTCAAGTTTCTTCTTTAAAGCATCCAAGAACACCATCAACTGGTCTTGGAATGATTGATTATCAAAGCTCTGATCATGAGCAACTAATGAAACGCCTGCGGACTGCACAATCTGTTGATGAGGTTAGCTATCCTCCTCAACATGCTTCCTGGTCGCTAGATGACCTACCGAGAAATGTATCTTGTACACTTCGTCAGGGATCCACTGTTATGAGCATGGATTTCCACCCTTCTCATCACACATTACTTGCTGTTGGCTGTAGTAATGGAGAAGTTACTCTGTGGGAAGTTGGGTTACGAGAGAAGCTGGTGTCCAAGCCATTCAAAGTATGGGAAATGACTGCTTGTTCTCCGACATTTCAGGCGGCTATTGTCAAAGATTCATCAATGTCTGTTAACCGCGTGTCATGGAGTCCAGACGGGAACTTAATCGGTGTTGCATTTACGAAACATTTGGTTCATCTGTATGCTTGTCAAGGGTCTAATGATCTACGCCAACATTTGGAGATTGAGGCCCACAGTGGAGGTGTGAATGACTTGGCATTCTCTCATCCAAACAAGCAATTGTGTGTTGTAACATGTGGAGATGATAAGATGATAAAGGTTTGGGATCTGGCTGGAAGGAAGCTTTTCCACTTTGAAGGTCATGAAGCACCTGTTTACTCGGTTTGCCCCCACCAGAAGGAGAATATTCAGTTTATATTTTCAACTGCTGTTGATGGAAAAATAAAAGCTTGGCTGTATGACAATGTGGGCTCCAGGGTTGACTATGATGCTCCAGGGCACTGGTGCACCACAATGCTGTACAGTGATGATGGAAATAGATTGTTCTCTTGTGGGACTAGTAAAGATGGTGACTCTTTCCTGGTTGAATGGAATGAAAGTGAAGGGGCAATAAAGAGGACCTATAGTGGGTTCAGAAAGAAATCTAATGGCATCGCGCAATTTGATACCACAAGAAATCACTTTTTGGCTGTTGGTGAAGATAGCCAGATAAAATTTTGGGATATGGATAATACAAATGTTCTTCTCAGTATAGAGGCTGATGGTGGACTACCACCTGTTCCTCGCTTGAGATTCAATAGGGAAGGGAATCTGCTTGTTGTTACCACGGCTGACAATGGATTCAAGGTTCTTGCAAATGCTGATGGTCTTAGATCCTTAAGAGCAATTGAAAATCGATCTTATGAAGCATCAAGAACACCTATTGAGATGAAGGTATCTACTTCGGCCATGGTTCCAAATATCAATCCTGCCATTAATAAAGTAGATCGTATGGATGCAAGCTCTCCTGCCAGACCTACTCAGATTCTTAATGGAGTTGATCCTATGGCAAGAAGTGTAGAAAAGCGAAGACTATTAGATGATGCATCCGAGAAAAATAAACGTTGGGAACTGGCAGAGATTCTTGATCCTGTTCACTGTCGAGTGGCTACGATGCCAGATAGTAAAGATCCTGCTAACAAGGTTGCTCGACTTCTTTACACAAATTCTGGTGCCGGCATTCTTGCTCTTGGTTCAAATGGGGTCCAGAAGCTATGGAAGTGGAGAAGCAGTGAACAAAATCCAACTGGAAAGGCCACTGCTAGTGTCGTTCCGCAACATTGGCAACCCAACAATGGTCTTTTGATGACTAATGATGTCCCTGAAAACTGTGAGGAAGCAGTTCCATGTATTGCACTCTCAAAGAATGACTCCTATGTGATGTCGGCATGTGGCGGAAAAGTGTCTCTGTTCAATATGATGACATTTAAGGTAATGACAACTTTCATGTCACCTCCACCAGCCTCAACCTTCCTAGCATTTCATCCTCTGGATAATAACATCATAGCAATTGGAACAGAAGATTCAACTATCCACATTTACAATGTTAGAGTTGATGAGGTCAAATCAAAACTGAAAGGCCACCAGAAACACATTACTGGATTAGCGTTTTCTGTTAACCTTAAGATCATGGTTTCATCAGGTGCTGATGCTCAAGTACGTGACATCCATTCTTTCTTATGTTATTGGAACAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

954

Amino Acids

106.32

Weight (kDa)

7.15

Isoelectric Point (pI)

44.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH_TPL PF17814 5 - 34 3e-11 LisH-like dimerisation domain
TPR1-like_2nd PF21889 37 - 161 7.5e-62 TPR1-like, CTLH-containing domain
zf_topless PF21359 164 - 204 2.3e-25 TOPLESS, zinc finger domain
Beta-prop_EML_2 PF23414 332 - 468 3e-08 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd PF25172 349 - 564 7.3e-10 WDR3 second beta-propeller domain
Beta-prop_RIG_2nd PF23775 359 - 500 2.6e-07 RIG second beta-propeller
Beta-prop_WDR3_1st PF25173 390 - 527 1e-13 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 404 - 561 3e-07 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 406 - 492 5.9e-09 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 408 - 623 1.5e-21 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 411 - 664 8.7e-21 THOC3 beta-propeller domain
WDR55 PF24796 412 - 527 3.8e-07 WDR55
Beta-prop_EML PF23409 449 - 653 1.1e-07 Echinoderm microtubule-associated protein first beta-propeller
WD40 PF00400 450 - 485 1.1e-06 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 458 - 530 3.2e-08 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 465 - 572 1.8e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 467 - 658 9.3e-10 Prp19 WD40 domain
WDR55 PF24796 513 - 658 4.7e-06 WDR55
Beta-prop_WDR3_1st PF25173 520 - 659 4.4e-07 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 817 - 943 3.5e-13 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 831 - 943 8.7e-13 WDR3 first beta-propeller domain
WDR55 PF24796 831 - 943 3.5e-06 WDR55
WD40_CDC20-Fz PF24807 832 - 942 8.8e-10 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 832 - 940 1.1e-08 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 833 - 943 5.5e-08 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 833 - 943 1.3e-10 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 834 - 943 2.5e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 837 - 943 7.3e-10 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 841 - 943 6.2e-07 THOC3 beta-propeller domain
Beta-prop_IFT140_1st PF23383 850 - 937 8e-06 IFT140 first beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 854 - 940 9.3e-08 WDR36/Utp21 first beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 856 - 939 3.8e-07 WDR90/POC16, second beta-propeller
Beta-prop_RIG_1st PF23770 864 - 927 4.4e-06 RIG first beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013792)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G16830
fragaria_vesca FvH4_4g18910 FvH4_4g18910
malus_domestica MD13G1162100.v1.1 MD16G1161900.v1.1
prunus_persica Prupe.1G129400_v2.0.a1
pyrus_communis pycom13g13830
rosa_chinensis RchiOBHm_Chr4g0423811
rosa_laevigata RLG00000007530
rosa_multiflora Rmu_sc0003369.1_g000018
rosa_roxburghii Rroxscaffold_5G00365920
rosa_rugosa Rorug04G0190900
rosa_samantha Rh4AG250300 Rh4BG255600 Rh4CG263700 Rh4DG250000
rosa_wichuraiana Rw4G021430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 2339
AccB7I CCANNNNNTGG 2 cut(s) 884, 1345
AccBSI CCGCTC 1 cut(s) 665
AccII CGCG 2 cut(s) 1245, 1784
AciI CCGC 8 cut(s) 414, 663, 719, 943, 1208, 1243, 2441, 2562
AclI AACGTT 1 cut(s) 2230
AclWI GGATC 8 cut(s) 28, 1038, 1051, 1461, 1995, 2162, 2249, 2291
AcoI YGGCCR 1 cut(s) 2073
AcsI RAATTY 3 cut(s) 59, 1841, 2332
AcuI CTGAAG 2 cut(s) 355, 461
AdeI CACNNNGTG 1 cut(s) 707
AfaI GTAC 7 cut(s) 75, 119, 167, 335, 1030, 1649, 2826
AfeI AGCGCT 1 cut(s) 252
AfiI CCNNNNNNNGG 6 cut(s) 623, 884, 1345, 1525, 1755, 2462
AflII CTTAAG 2 cut(s) 2005, 2789
AflIII ACRYGT 1 cut(s) 1421
AhlI ACTAGT 1 cut(s) 1685
AjnI CCWGG 4 cut(s) 992, 1601, 1621, 1707
AleI CACNNNNGTG 1 cut(s) 185
AloI GAACNNNNNNTCC 2 cut(s) 1959, 1991
Alw21I GWGCWC 3 cut(s) 631, 703, 1637
Alw26I GTCTC 2 cut(s) 820, 2577
Alw44I GTGCAC 1 cut(s) 1633
AlwI GGATC 8 cut(s) 28, 1038, 1051, 1461, 1995, 2162, 2249, 2291
AlwNI CAGNNNCTG 1 cut(s) 2813
Aor51HI AGCGCT 1 cut(s) 252
AoxI GGCC 5 cut(s) 569, 1356, 2073, 2421, 2746
ApaLI GTGCAC 1 cut(s) 1633
ApeKI GCWGC 8 cut(s) 544, 725, 728, 731, 734, 737, 740, 743
ApoI RAATTY 3 cut(s) 59, 1841, 2332
AseI ATTAAT 1 cut(s) 2103
Asp700I GAANNNNTTC 4 cut(s) 119, 215, 1472, 2508
AspLEI GCGC 2 cut(s) 253, 1786
AspS9I GGNCC 5 cut(s) 601, 710, 1357, 1746, 2371
AsuC2I CCSGG 1 cut(s) 175
AsuHPI GGTGA 7 cut(s) 172, 304, 413, 816, 1709, 1835, 2610
AvaII GGWCC 4 cut(s) 601, 710, 1746, 2371
BaeGI GKGCMC 2 cut(s) 1629, 1637
BamHI GGATCC 1 cut(s) 1043
BanI GGYRCC 1 cut(s) 2339
BanII GRGCYC 2 cut(s) 631, 1601
BarI GAAGNNNNNNTAC 2 cut(s) 1122, 1154
BauI CACGAG 2 cut(s) 607, 630
BbsI GAAGAC 1 cut(s) 2203
Bbv12I GWGCWC 3 cut(s) 631, 703, 1637
BbvI GCAGC 8 cut(s) 556, 712, 715, 718, 721, 724, 730, 752
BccI CCATC 7 cut(s) 762, 886, 1556, 1652, 1688, 1883, 1985
BceAI ACGGC 1 cut(s) 1971
BciT130I CCWGG 4 cut(s) 994, 1603, 1623, 1709
BclI TGATCA 2 cut(s) 522, 916
BcnI CCSGG 1 cut(s) 175
BcoDI GTCTC 2 cut(s) 820, 2577
BcuI ACTAGT 1 cut(s) 1685
BfaI CTAG 6 cut(s) 417, 1001, 1686, 2430, 2642, 2863
BfmI CTRYAG 3 cut(s) 738, 1102, 1750
BfoI RGCGCY 1 cut(s) 254
BfrI CTTAAG 2 cut(s) 2005, 2789
BglII AGATCT 1 cut(s) 213
BmcAI AGTACT 1 cut(s) 119
Bme1390I CCNGG 5 cut(s) 175, 994, 1603, 1623, 1709
Bme18I GGWCC 4 cut(s) 601, 710, 1746, 2371
BmgT120I GGNCC 5 cut(s) 601, 710, 1357, 1746, 2371
BmiI GGNNCC 5 cut(s) 1045, 1600, 2082, 2341, 2372
BmrFI CCNGG 5 cut(s) 175, 994, 1603, 1623, 1709
BpiI GAAGAC 1 cut(s) 2203
BpmI CTGGAG 3 cut(s) 627, 1585, 1605
BpuEI CTTGAG 4 cut(s) 68, 104, 1936, 2805
BpuMI CCSGG 1 cut(s) 175
Bsa29I ATCGAT 1 cut(s) 2023
BsaAI YACGTR 1 cut(s) 2828
BsaI GGTCTC 1 cut(s) 820
BsaJI CCNNGG 6 cut(s) 183, 566, 1602, 1622, 1953, 2076
BsaWI WCCGGW 1 cut(s) 811
BsaXI ACNNNNNCTCC 6 cut(s) 119, 149, 673, 703, 2383, 2413
Bsc4I CCNNNNNNNGG 6 cut(s) 623, 884, 1345, 1525, 1755, 2462
Bse118I RCCGGY 2 cut(s) 721, 2342
Bse1I ACTGG 8 cut(s) 294, 644, 656, 889, 1634, 2244, 2419, 2770
BseBI CCWGG 4 cut(s) 994, 1603, 1623, 1709
BseCI ATCGAT 1 cut(s) 2023
BseDI CCNNGG 6 cut(s) 183, 566, 1602, 1622, 1953, 2076
BseGI GGATG 8 cut(s) 773, 777, 865, 1393, 2128, 2213, 2650, 2832
BseLI CCNNNNNNNGG 6 cut(s) 623, 884, 1345, 1525, 1755, 2462
BseMII CTCAG 2 cut(s) 1888, 2162
BseNI ACTGG 8 cut(s) 294, 644, 656, 889, 1634, 2244, 2419, 2770
BseRI GAGGAG 6 cut(s) 65, 68, 140, 606, 681, 969
BseSI GKGCMC 2 cut(s) 1629, 1637
BseXI GCAGC 8 cut(s) 556, 712, 715, 718, 721, 724, 730, 752
BsgI GTGCAG 1 cut(s) 933
Bsh1236I CGCG 2 cut(s) 1245, 1784
BshFI GGCC 5 cut(s) 571, 1358, 2075, 2423, 2748
BshNI GGYRCC 1 cut(s) 2339
BshVI ATCGAT 1 cut(s) 2023
BsiHKAI GWGCWC 3 cut(s) 631, 703, 1637
BsiSI CCGG 4 cut(s) 174, 722, 812, 2343
BslFI GGGAC 3 cut(s) 164, 1696, 2471
BslI CCNNNNNNNGG 6 cut(s) 623, 884, 1345, 1525, 1755, 2462
BsmAI GTCTC 2 cut(s) 820, 2577
BsmFI GGGAC 3 cut(s) 164, 1696, 2471
BsmI GAATGC 3 cut(s) 760, 1385, 2346
BsnI GGCC 5 cut(s) 571, 1358, 2075, 2423, 2748
Bso31I GGTCTC 1 cut(s) 820
Bsp1286I GDGCHC 5 cut(s) 631, 703, 1601, 1629, 1637
Bsp1407I TGTACA 4 cut(s) 73, 333, 1028, 1647
Bsp19I CCATGG 1 cut(s) 2076
BspACI CCGC 8 cut(s) 414, 663, 719, 943, 1208, 1243, 2441, 2562
BspANI GGCC 5 cut(s) 571, 1358, 2075, 2423, 2748
BspCNI CTCAG 2 cut(s) 1887, 2161
BspDI ATCGAT 1 cut(s) 2023
BspFNI CGCG 2 cut(s) 1245, 1784
BspHI TCATGA 2 cut(s) 919, 1489
BspLI GGNNCC 5 cut(s) 1045, 1600, 2082, 2341, 2372
BspMAI CTGCAG 1 cut(s) 742
BspPI GGATC 8 cut(s) 28, 1038, 1051, 1461, 1995, 2162, 2249, 2291
BspT107I GGYRCC 1 cut(s) 2339
BspTI CTTAAG 2 cut(s) 2005, 2789
BspTNI GGTCTC 1 cut(s) 820
BsrBI CCGCTC 1 cut(s) 665
BsrFI RCCGGY 2 cut(s) 721, 2342
BsrGI TGTACA 4 cut(s) 73, 333, 1028, 1647
BsrI ACTGG 8 cut(s) 294, 644, 656, 889, 1634, 2244, 2419, 2770
BssAI RCCGGY 2 cut(s) 721, 2342
BssECI CCNNGG 6 cut(s) 183, 566, 1602, 1622, 1953, 2076
BssSI CACGAG 2 cut(s) 607, 630
BssT1I CCWWGG 3 cut(s) 183, 566, 2076
Bst2BI CACGAG 2 cut(s) 607, 630
Bst2UI CCWGG 4 cut(s) 994, 1603, 1623, 1709
Bst4CI ACNGT 5 cut(s) 1051, 1364, 1652, 2267, 2498
Bst6I CTCTTC 1 cut(s) 657
BstAFI CTTAAG 2 cut(s) 2005, 2789
BstAUI TGTACA 4 cut(s) 73, 333, 1028, 1647
BstBAI YACGTR 1 cut(s) 2828
BstC8I GCNNGC 5 cut(s) 512, 723, 941, 2128, 2344
BstDEI CTNAG 4 cut(s) 11, 1874, 1997, 2148
BstDSI CCRYGG 2 cut(s) 1953, 2076
BstF5I GGATG 8 cut(s) 773, 777, 865, 1393, 2128, 2213, 2650, 2832
BstFNI CGCG 2 cut(s) 1245, 1784
BstH2I RGCGCY 1 cut(s) 254
BstHHI GCGC 2 cut(s) 253, 1786
BstMAI GTCTC 2 cut(s) 820, 2577
BstNI CCWGG 4 cut(s) 994, 1603, 1623, 1709
BstNSI RCATGY 3 cut(s) 989, 1425, 2559
BstSCI CCNGG 5 cut(s) 173, 992, 1601, 1621, 1707
BstSFI CTRYAG 3 cut(s) 738, 1102, 1750
BstSLI GKGCMC 2 cut(s) 1629, 1637
BstUI CGCG 2 cut(s) 1245, 1784
BstV1I GCAGC 8 cut(s) 556, 712, 715, 718, 721, 724, 730, 752
BstV2I GAAGAC 1 cut(s) 2203
BstX2I RGATCY 5 cut(s) 213, 1043, 1453, 2000, 2296
BstXI CCANNNNNNTGG 1 cut(s) 1629
BstYI RGATCY 5 cut(s) 213, 1043, 1453, 2000, 2296
Bsu15I ATCGAT 1 cut(s) 2023
BsuRI GGCC 5 cut(s) 571, 1358, 2075, 2423, 2748
BsuTUI ATCGAT 1 cut(s) 2023
BtgI CCRYGG 2 cut(s) 1953, 2076
BtgZI GCGATG 1 cut(s) 1765
BtsCI GGATG 8 cut(s) 773, 777, 865, 1393, 2128, 2213, 2650, 2832
BtsI GCAGTG 2 cut(s) 2404, 2424
BtsIMutI CAGTG 7 cut(s) 1047, 1369, 1627, 1657, 2263, 2404, 2424
Cac8I GCNNGC 5 cut(s) 512, 723, 941, 2128, 2344
CaiI CAGNNNCTG 1 cut(s) 2813
CciI TCATGA 2 cut(s) 919, 1489
CfoI GCGC 2 cut(s) 253, 1786
Cfr10I RCCGGY 2 cut(s) 721, 2342
Cfr13I GGNCC 5 cut(s) 601, 710, 1357, 1746, 2371
ClaI ATCGAT 1 cut(s) 2023
Csp6I GTAC 7 cut(s) 74, 118, 166, 334, 1029, 1648, 2825
CviQI GTAC 7 cut(s) 74, 118, 166, 334, 1029, 1648, 2825
DdeI CTNAG 4 cut(s) 11, 1874, 1997, 2148
DraI TTTAAA 2 cut(s) 537, 861
DraIII CACNNNGTG 1 cut(s) 707
EaeI YGGCCR 1 cut(s) 2073
Eam1104I CTCTTC 1 cut(s) 657
EarI CTCTTC 1 cut(s) 657
EciI GGCGGA 1 cut(s) 2577
Ecl136II GAGCTC 1 cut(s) 629
Eco130I CCWWGG 3 cut(s) 183, 566, 2076
Eco24I GRGCYC 2 cut(s) 631, 1601
Eco31I GGTCTC 1 cut(s) 820
Eco47I GGWCC 4 cut(s) 601, 710, 1746, 2371
Eco47III AGCGCT 1 cut(s) 252
Eco53kI GAGCTC 1 cut(s) 629
Eco57I CTGAAG 2 cut(s) 355, 461
EcoICRI GAGCTC 1 cut(s) 629
EcoO109I RGGNCCY 1 cut(s) 1746
EcoRII CCWGG 4 cut(s) 992, 1601, 1621, 1707
EcoT14I CCWWGG 3 cut(s) 183, 566, 2076
EcoT22I ATGCAT 1 cut(s) 2215
EcoT38I GRGCYC 2 cut(s) 631, 1601
ErhI CCWWGG 3 cut(s) 183, 566, 2076
FalI AAGNNNNNCTT 2 cut(s) 203, 235
FaqI GGGAC 3 cut(s) 164, 1696, 2471
FauI CCCGC 1 cut(s) 726
FbaI TGATCA 2 cut(s) 522, 916
FokI GGATG 8 cut(s) 780, 784, 852, 1380, 2135, 2200, 2637, 2819
FriOI GRGCYC 2 cut(s) 631, 1601
FspBI CTAG 6 cut(s) 417, 1001, 1686, 2430, 2642, 2863
GlaI GCGC 2 cut(s) 252, 1785
GsuI CTGGAG 3 cut(s) 627, 1585, 1605
HaeII RGCGCY 1 cut(s) 254
HaeIII GGCC 5 cut(s) 571, 1358, 2075, 2423, 2748
HapII CCGG 4 cut(s) 174, 722, 812, 2343
HhaI GCGC 2 cut(s) 253, 1786
Hin6I GCGC 2 cut(s) 251, 1784
HinP1I GCGC 2 cut(s) 251, 1784
HincII GTYRAC 4 cut(s) 357, 1240, 1609, 2785
HindII GTYRAC 4 cut(s) 357, 1240, 1609, 2785
HindIII AAGCTT 2 cut(s) 1469, 1575
HpaI GTTAAC 2 cut(s) 1240, 2785
HpaII CCGG 4 cut(s) 174, 722, 812, 2343
HphI GGTGA 7 cut(s) 172, 304, 413, 816, 1709, 1835, 2610
Hpy188I TCNGA 9 cut(s) 12, 230, 374, 473, 916, 1197, 1763, 2151, 2218
Hpy99I CGWCG 1 cut(s) 319
HpyCH4III ACNGT 5 cut(s) 1051, 1364, 1652, 2267, 2498
HpyCH4IV ACGT 3 cut(s) 317, 2230, 2827
HpyF3I CTNAG 4 cut(s) 11, 1874, 1997, 2148
HpySE526I ACGT 3 cut(s) 317, 2230, 2827
HspAI GCGC 2 cut(s) 251, 1784
KroI GCCGGC 2 cut(s) 721, 2342
KroNI GCCGGC 2 cut(s) 723, 2344
Ksp22I TGATCA 2 cut(s) 522, 916
KspAI GTTAAC 2 cut(s) 1240, 2785
LmnI GCTCC 7 cut(s) 19, 626, 694, 698, 814, 1604, 1624
Lsp1109I GCAGC 8 cut(s) 556, 712, 715, 718, 721, 724, 730, 752
MaeI CTAG 6 cut(s) 417, 1001, 1686, 2430, 2642, 2863
MaeII ACGT 3 cut(s) 317, 2230, 2827
MaeIII GTNAC 8 cut(s) 646, 1117, 1137, 1417, 1697, 1948, 2616, 2828
MbiI CCGCTC 1 cut(s) 665
MfeI CAATTG 3 cut(s) 1406, 2013, 2676
MflI RGATCY 5 cut(s) 213, 1043, 1453, 2000, 2296
MhlI GDGCHC 5 cut(s) 631, 703, 1601, 1629, 1637
MlyI GAGTC 6 cut(s) 101, 352, 634, 1264, 1694, 2531
MmeI TCCRAC 2 cut(s) 1220, 2435
Mph1103I ATGCAT 1 cut(s) 2215
MroNI GCCGGC 2 cut(s) 721, 2342
MroXI GAANNNNTTC 4 cut(s) 119, 215, 1472, 2508
MslI CAYNNNNRTG 4 cut(s) 185, 1052, 1308, 1640
MspA1I CMGCKG 1 cut(s) 743
MspCI CTTAAG 2 cut(s) 2005, 2789
MspI CCGG 4 cut(s) 174, 722, 812, 2343
MspR9I CCNGG 5 cut(s) 175, 994, 1603, 1623, 1709
MunI CAATTG 3 cut(s) 1406, 2013, 2676
Mva1269I GAATGC 3 cut(s) 760, 1385, 2346
MvaI CCWGG 4 cut(s) 994, 1603, 1623, 1709
MvnI CGCG 2 cut(s) 1245, 1784
NaeI GCCGGC 2 cut(s) 723, 2344
NciI CCSGG 1 cut(s) 175
NcoI CCATGG 1 cut(s) 2076
NgoMIV GCCGGC 2 cut(s) 721, 2342
NlaIV GGNNCC 5 cut(s) 1045, 1600, 2082, 2341, 2372
NmuCI GTSAC 3 cut(s) 1697, 2616, 2828
NsiI ATGCAT 1 cut(s) 2215
NspI RCATGY 3 cut(s) 989, 1425, 2559
OliI CACNNNNGTG 1 cut(s) 185
PagI TCATGA 2 cut(s) 919, 1489
PciI ACATGT 1 cut(s) 1421
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PctI GAATGC 3 cut(s) 760, 1385, 2346
PdiI GCCGGC 2 cut(s) 723, 2344
PdmI GAANNNNTTC 4 cut(s) 119, 215, 1472, 2508
PfeI GAWTC 9 cut(s) 99, 196, 1223, 1920, 1936, 1968, 2152, 2248, 2690
PflMI CCANNNNNTGG 2 cut(s) 884, 1345
PleI GAGTC 6 cut(s) 100, 352, 634, 1263, 1694, 2531
PpsI GAGTC 6 cut(s) 100, 352, 634, 1263, 1694, 2531
Ppu21I YACGTR 1 cut(s) 2828
PpuMI RGGWCCY 1 cut(s) 1746
PscI ACATGT 1 cut(s) 1421
PshBI ATTAAT 1 cut(s) 2103
Psp124BI GAGCTC 1 cut(s) 631
Psp1406I AACGTT 1 cut(s) 2230
Psp5II RGGWCCY 1 cut(s) 1746
Psp6I CCWGG 4 cut(s) 992, 1601, 1621, 1707
PspGI CCWGG 4 cut(s) 992, 1601, 1621, 1707
PspN4I GGNNCC 5 cut(s) 1045, 1600, 2082, 2341, 2372
PspPI GGNCC 5 cut(s) 601, 710, 1357, 1746, 2371
PspPPI RGGWCCY 1 cut(s) 1746
PstI CTGCAG 1 cut(s) 742
PstNI CAGNNNCTG 1 cut(s) 2813
PsuI RGATCY 5 cut(s) 213, 1043, 1453, 2000, 2296
PvuII CAGCTG 1 cut(s) 743
RsaI GTAC 7 cut(s) 75, 119, 167, 335, 1030, 1649, 2826
RsaNI GTAC 7 cut(s) 74, 118, 166, 334, 1029, 1648, 2825
RseI CAYNNNNRTG 4 cut(s) 185, 1052, 1308, 1640
SacI GAGCTC 1 cut(s) 631
Sau96I GGNCC 5 cut(s) 601, 710, 1357, 1746, 2371
ScaI AGTACT 1 cut(s) 119
SchI GAGTC 6 cut(s) 101, 352, 634, 1264, 1694, 2531
ScrFI CCNGG 5 cut(s) 175, 994, 1603, 1623, 1709
SduI GDGCHC 5 cut(s) 631, 703, 1601, 1629, 1637
SfcI CTRYAG 3 cut(s) 738, 1102, 1750
SinI GGWCC 4 cut(s) 601, 710, 1746, 2371
SmiMI CAYNNNNRTG 4 cut(s) 185, 1052, 1308, 1640
SmlI CTYRAG 6 cut(s) 47, 83, 1915, 2005, 2789, 2820
SmoI CTYRAG 6 cut(s) 47, 83, 1915, 2005, 2789, 2820
SpeI ACTAGT 1 cut(s) 1685
SsiI CCGC 8 cut(s) 414, 663, 719, 943, 1208, 1243, 2441, 2562
SspI AATATT 1 cut(s) 1534
SspMI CTAG 6 cut(s) 417, 1001, 1686, 2430, 2642, 2863
SstI GAGCTC 1 cut(s) 631
StyD4I CCNGG 5 cut(s) 173, 992, 1601, 1621, 1707
StyI CCWWGG 3 cut(s) 183, 566, 2076
TaaI ACNGT 5 cut(s) 1051, 1364, 1652, 2267, 2498
TaiI ACGT 3 cut(s) 320, 2233, 2830
TatI WGTACW 5 cut(s) 73, 117, 333, 1028, 1647
TauI GCSGC 2 cut(s) 665, 1211
TfiI GAWTC 9 cut(s) 99, 196, 1223, 1920, 1936, 1968, 2152, 2248, 2690
TscAI CASTG 7 cut(s) 1054, 1369, 1634, 1657, 2270, 2404, 2431
TseFI GTSAC 3 cut(s) 1697, 2616, 2828
TseI GCWGC 8 cut(s) 544, 725, 728, 731, 734, 737, 740, 743
Tsp45I GTSAC 3 cut(s) 1697, 2616, 2828
TspGWI ACGGA 1 cut(s) 777
TspRI CASTG 7 cut(s) 1054, 1369, 1634, 1657, 2270, 2404, 2431
Van91I CCANNNNNTGG 2 cut(s) 884, 1345
Vha464I CTTAAG 2 cut(s) 2005, 2789
VneI GTGCAC 1 cut(s) 1633
VpaK11BI GGWCC 4 cut(s) 601, 710, 1746, 2371
VspI ATTAAT 1 cut(s) 2103
XapI RAATTY 3 cut(s) 59, 1841, 2332
XceI RCATGY 3 cut(s) 989, 1425, 2559
XcmI CCANNNNNNNNNTGG 1 cut(s) 1170
XmnI GAANNNNTTC 4 cut(s) 119, 215, 1472, 2508
XspI CTAG 6 cut(s) 417, 1001, 1686, 2430, 2642, 2863
ZrmI AGTACT 1 cut(s) 119
Zsp2I ATGCAT 1 cut(s) 2215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.