Rw4G021430

Topless-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0013792
Physical Location & Seq
Forward (+)
46108351 .. 46115771
7421 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G021430.1

Sequence Viewer

Length: 3297 bp
ATGTCATCTCTGAGCAGGGAGCTGGTTTTCCTGATCCTTCAATTTCTTGAGGAGGAGAAATTCAAGGAGGCTGTACATAGGCTTGAGCAAGAGTCTGGATTCTTCTTCAACATGAAGTACTTCGAGGAGAAAGCGTTGCAGGGAGAGTGGGACGAGGTGGAGAAGTACCTGTCCGGGTTCACCAAGGTGGACGAGAATCGCTACTCTATGAAGATCTTCTTCGAGGTCAGAAAGCAGAAGTATCTCGAAGCGCTCGACAGGCAAGACAGGGCGAAAGCTGTTGAGATACTGGTGAAGGATTTGAAGGTGTTTTCGACGTTTAATGAGGACTTGTACAAGGAAATCACTCATCTGTTGACTCTTGACAACTTCAGAGAAAATGAGCAGTTGTCGAAGTATGGTGATACTAAATCCGCTAGAAGCATAATGCTGGTAGAGCTGAAGAAATTGATAGAAGCATCACGCTTGCGAACTTTGATCAATCAAAGTTTAAATTGGCAGCATCAGCTATGCAAAAACCCAAGGCCAAATCCTGACATTAAGACCTTATTCATGGACCACTCGTGTTCTCCTCCAAATGGAGCTCGTGCATCGACTCCAGTTACTCTTCCAGTTGCCGCTCTTACAAAGCCTTTAACCTATGCTCCTCTTGGAGCACATGGTGGACCTTTCCCGCCGGCTGCTGCTGCTGCTGCAGCTGCCAATGCGAATGCTTTAGCAGGATGGATGTCCAATGCTAATCCGTCCTTGTCTATGCAATCAGCTCCGGTCTCTGCTTCACCCCTGCCTGTTCAACTTGGTCAAGTTTCTTCTTTAAAGCATCCAAGAACACCATCAACTGGTCTTGGAATGATTGATTATCAAAGCTCTGATCATGAGCAACTAATGAAACGCCTGCGGACTGCACAATCTGTTGATGAGGTTAGCTATCCTCCTCAACATGCTTCCTGGTCGCTAGATGACCTACCGAGAAATGTATCTTGTACACTTCGTCAGGGATCCACTGTTATGAGCATGGATTTCCACCCTTCTCATCACACATTACTTGCTGTTGGCTGTAGTAATGGAGAAGTTACTCTGTGGGAAGTTGGGTTACGAGAGAAGCTGGTGTCCAAGCCATTCAAAGTATGGGAAATGACTGCTTGTTCTCCGACATTTCAGGCGGCTATTGTCAAAGATTCATCAATGTCTGTTAACCGCGTGTCATGGAGTCCAGACGGGAACTTAATCGGTGTTGCATTTACGAAACATTTGGTTCATCTGTATGCTTGTCAAGGGTCTAATGATCTACGCCAACATTTGGAGATTGAGGCCCACAGTGGAGGTGTGAATGACTTGGCATTCTCTCATCCAAACAAGCAATTGTGTGTTGTAACATGTGGAGATGATAAGATGATAAAGGTTTGGGATCTGGCTGGAAGGAAGCTTTTCCACTTTGAAGGTCATGAAGCACCTGTTTACTCGGTTTGCCCCCACCAGAAGGAGAATATTCAGTTTATATTTTCAACTGCTGTTGATGGAAAAATAAAAGCTTGGCTGTATGACAATGTGGGCTCCAGGGTTGACTATGATGCTCCAGGGCACTGGTGCACCACAATGCTGTACAGTGATGATGGAAATAGATTGTTCTCTTGTGGGACTAGTAAAGATGGGGCAATAAAGAGGACCTATAGTGGGTTCAGAAAGAAATCTAATGGCATCGCGCAATTTGATACCACAAGAAATCACTTTTTGGCTGTTGGTGAAGATAGCCAGATAAAATTTTGGGATATGGATAATACAAATGTTCTTCTCAGTATAGAGGCTGATGGTGGACTACCACCTGTTCCTCGCTTGAGATTCAATAGGGAAGGGAATCTGCTTGTTGTTACCACGGCTGACAATGGATTCAAGGTTCTTGCAAATGCTGATGGTCTTAGATCCTTAAGAGCAATTGAAAATCGATCTTATGAAGCATCAAGAACACCTATTGAGATGAAGGTATCTACTTCGGCCATGGTTCCAAATATCAATCCTGCCATTAATAAAGTAGATCGTATGGATGCAAGCTCTCCTGCCAGACCTACTCAGATTCTTAATGGAGTTGATCCTATGGCAAGAAGTGTAGAAAAGCGAAGACTATTAGATGATGCATCCGAGAAAAATAAACGTTGGGAACTGGCAGAGATTCTTGATCCTGTTCACTGTCGAGTGGCTACGATGCCAGATAGTAAAGATCCTGCTAACAAGGTTGCTCGACTTCTTTACACAAATTCTGGTGCCGGCATTCTTGCTCTTGGTTCAAATGGGGTCCAGAAGCTATGGAAGTGGAGAAGCAGTGAACAAAATCCAACTGGAAAGGCCACTGCTAGTGTCGTTCCGCAACATTGGCAACCCAACAATGGTCTTTTGATGACTAATGATGTCCCTGAAAACTGTGAGGAAGCAGTTCCATGTATTGCACTCTCAAAGAATGACTCCTATGTGATGTCGGCATGTGGCGGAAAAGTGTCTCTGTTCAATATGATGACATTTAAGGTAATGACAACTTTCATGTCACCTCCACCAGCCTCAACCTTCCTAGCATTTCATCCTCTGGATAATAACATCATAGCAATTGGAACAGAAGATTCAACTATCCACATTTACAATGTTAGAGTTGATGAGGTCAAATCAAAACTGAAAGGCCACCAGAAACACATTACTGGATTAGCGTTTTCTGTTAACCTTAAGATCATGGTTTCATCAGGTGCTGATGCTCAACTATGCTTTTGGAACATGGATACGTGGGAGAAGAGGAAGTCGCTGACAATTCAACTGCCAGCCGGAAAGGCGCCTGTTGGTGACACACAAGTGCAATTCTATTCTGATCAAACTCGGTTGTTGGTATGCCATGAGACTCAACTAGCACTATATGATGCTGCCAAAGCAGAATGCATTCGACAGTGGATGCCGCAAGATGTACTGCCTGCACCAATAACGTGTGCAGCATATTCCCCCAATAGTCAACTAGTCTATGCGGGTTTTACTGATGGCAATATTGGAGTATTTGATGCTGATAGTCTGAAATTAAGATGTCGTATTTCCATGTCAGTGTACTTGGCACAAGCATCCCCAAGCAGCCAAACTGTGTTCCCGCTAGCTCTTACAGCACATCTGCAGGAGCCATATCAGTTCGCCGTTGGACTGACAGATGGATCTGTTAAAGTTATAGAGCCCTCAGACGCTGAGGGGAAGTGGGGAGTTACAGTGCCTGTTGATAACGGAGCACAAAACGGCTGGACAGCAACATCTTCGTCTAACAATCCTGCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

1098

Amino Acids

121.49

Weight (kDa)

6.76

Isoelectric Point (pI)

46.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH_TPL PF17814 5 - 34 3.5e-11 LisH-like dimerisation domain
TPR1-like_2nd PF21889 37 - 156 8e-58 TPR1-like, CTLH-containing domain
zf_topless PF21359 151 - 189 4e-24 TOPLESS, zinc finger domain
Beta-prop_EML_2 PF23414 317 - 453 3.2e-08 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd PF25172 334 - 549 6.2e-10 WDR3 second beta-propeller domain
Beta-prop_RIG_2nd PF23775 344 - 485 3.4e-07 RIG second beta-propeller
Beta-prop_WDR3_1st PF25173 375 - 547 1.7e-14 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 388 - 546 2.8e-07 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 391 - 478 5.8e-09 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 393 - 554 5.4e-17 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 396 - 637 7.3e-18 THOC3 beta-propeller domain
WDR55 PF24796 397 - 512 4.4e-07 WDR55
WD40 PF00400 435 - 470 1.3e-06 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 443 - 515 3.8e-08 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 450 - 554 1.4e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 452 - 630 3.6e-08 Prp19 WD40 domain
WD40_Prp19 PF24814 790 - 931 2.2e-14 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 804 - 929 4.3e-16 WDR3 first beta-propeller domain
WDR55 PF24796 804 - 921 1.6e-08 WDR55
Beta-prop_EML_2 PF23414 805 - 928 9.9e-13 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 805 - 930 1.2e-12 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 806 - 1016 2.6e-18 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 806 - 1014 1.9e-21 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 807 - 922 8.8e-09 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 810 - 928 1.1e-13 WDR3 second beta-propeller domain
Beta-prop_IFT140_1st PF23383 823 - 922 6.7e-09 IFT140 first beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 827 - 950 9.3e-12 WDR36/Utp21 first beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 829 - 922 3.3e-08 WDR90/POC16, second beta-propeller
Beta-prop_RIG_1st PF23770 837 - 900 3.2e-06 RIG first beta-propeller
WD40_Gbeta PF25391 857 - 1012 1.2e-06 G protein beta WD-40 repeat protein
WD40_Prp19 PF24814 883 - 1017 7.6e-10 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 890 - 1020 3.4e-07 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013792)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G16830
fragaria_vesca FvH4_4g18910 FvH4_4g18910
malus_domestica MD13G1162100.v1.1 MD16G1161900.v1.1
prunus_persica Prupe.1G129400_v2.0.a1
pyrus_communis pycom13g13830
rosa_chinensis RchiOBHm_Chr4g0423811
rosa_laevigata RLG00000007530
rosa_multiflora Rmu_sc0003369.1_g000018
rosa_roxburghii Rroxscaffold_5G00365920
rosa_rugosa Rorug04G0190900
rosa_samantha Rh4AG250300 Rh4BG255600 Rh4CG263700 Rh4DG250000
rosa_wichuraiana Rw4G021430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 2258, 2812
AccB7I CCANNNNNTGG 2 cut(s) 839, 1300
AccBSI CCGCTC 1 cut(s) 620
AccII CGCG 2 cut(s) 1200, 1703
AclI AACGTT 1 cut(s) 2149
AclWI GGATC 9 cut(s) 28, 993, 1006, 1416, 1914, 2081, 2168, 2210, 3184
AcoI YGGCCR 1 cut(s) 1992
AcsI RAATTY 3 cut(s) 59, 1760, 2251
AcuI CTGAAG 2 cut(s) 355, 461
AcyI GRCGYC 1 cut(s) 2813
AdeI CACNNNGTG 1 cut(s) 662
AfaI GTAC 8 cut(s) 75, 119, 167, 335, 985, 1604, 2943, 3077
AfeI AGCGCT 1 cut(s) 252
AfiI CCNNNNNNNGG 6 cut(s) 578, 839, 1300, 1480, 1674, 2381
AflII CTTAAG 2 cut(s) 1924, 2708
AflIII ACRYGT 2 cut(s) 1376, 2960
AhlI ACTAGT 2 cut(s) 1640, 2989
AjnI CCWGG 3 cut(s) 947, 1556, 1576
AleI CACNNNNGTG 2 cut(s) 185, 2831
AloI GAACNNNNNNTCC 2 cut(s) 1878, 1910
Alw21I GWGCWC 4 cut(s) 586, 658, 1592, 3250
Alw26I GTCTC 3 cut(s) 775, 2496, 2870
Alw44I GTGCAC 1 cut(s) 1588
AlwI GGATC 9 cut(s) 28, 993, 1006, 1416, 1914, 2081, 2168, 2210, 3184
AlwNI CAGNNNCTG 3 cut(s) 2732, 3206, 3233
Aor51HI AGCGCT 1 cut(s) 252
AoxI GGCC 5 cut(s) 524, 1311, 1992, 2340, 2665
ApaLI GTGCAC 1 cut(s) 1588
ApoI RAATTY 3 cut(s) 59, 1760, 2251
AseI ATTAAT 1 cut(s) 2022
Asp700I GAANNNNTTC 5 cut(s) 119, 215, 1427, 2427, 2916
AspLEI GCGC 3 cut(s) 253, 1705, 2815
AspS9I GGNCC 5 cut(s) 556, 665, 1312, 1665, 2290
AsuC2I CCSGG 1 cut(s) 175
AsuHPI GGTGA 7 cut(s) 172, 304, 413, 771, 1754, 2529, 2834
AsuNHI GCTAGC 1 cut(s) 3118
AvaII GGWCC 4 cut(s) 556, 665, 1665, 2290
BaeGI GKGCMC 2 cut(s) 1584, 1592
BamHI GGATCC 1 cut(s) 998
BanI GGYRCC 2 cut(s) 2258, 2812
BanII GRGCYC 3 cut(s) 586, 1556, 3198
BarI GAAGNNNNNNTAC 2 cut(s) 1077, 1109
BauI CACGAG 2 cut(s) 562, 585
BbsI GAAGAC 1 cut(s) 2122
Bbv12I GWGCWC 4 cut(s) 586, 658, 1592, 3250
BbvCI CCTCAGC 1 cut(s) 3207
BccI CCATC 9 cut(s) 717, 841, 1511, 1607, 1643, 1802, 1904, 3005, 3167
BceAI ACGGC 3 cut(s) 1890, 3143, 3271
BcgI CGANNNNNNTGC 2 cut(s) 3255, 3289
BciT130I CCWGG 3 cut(s) 949, 1558, 1578
BciVI GTATCC 1 cut(s) 2755
BclI TGATCA 3 cut(s) 477, 871, 2848
BcnI CCSGG 1 cut(s) 175
BcoDI GTCTC 3 cut(s) 775, 2496, 2870
BcuI ACTAGT 2 cut(s) 1640, 2989
BfaI CTAG 8 cut(s) 417, 956, 1641, 2349, 2561, 2885, 2990, 3119
BfmI CTRYAG 4 cut(s) 693, 1057, 1669, 3137
BfoI RGCGCY 2 cut(s) 254, 2816
BfrI CTTAAG 2 cut(s) 1924, 2708
BfuI GTATCC 1 cut(s) 2755
BglI GCCNNNNNGGC 1 cut(s) 2810
BglII AGATCT 1 cut(s) 213
BmcAI AGTACT 1 cut(s) 119
Bme1390I CCNGG 4 cut(s) 175, 949, 1558, 1578
Bme18I GGWCC 4 cut(s) 556, 665, 1665, 2290
BmgT120I GGNCC 5 cut(s) 556, 665, 1312, 1665, 2290
BmiI GGNNCC 7 cut(s) 1000, 1555, 2001, 2260, 2291, 2814, 3144
BmrFI CCNGG 4 cut(s) 175, 949, 1558, 1578
BmtI GCTAGC 1 cut(s) 3122
BpiI GAAGAC 1 cut(s) 2122
BpmI CTGGAG 3 cut(s) 582, 1540, 1560
Bpu10I CCTNAGC 1 cut(s) 3207
BpuEI CTTGAG 3 cut(s) 68, 104, 1855
BpuMI CCSGG 1 cut(s) 175
Bsa29I ATCGAT 1 cut(s) 1942
BsaAI YACGTR 1 cut(s) 2766
BsaHI GRCGYC 1 cut(s) 2813
BsaI GGTCTC 1 cut(s) 775
BsaJI CCNNGG 6 cut(s) 183, 521, 1557, 1577, 1872, 1995
BsaWI WCCGGW 1 cut(s) 766
BsaXI ACNNNNNCTCC 8 cut(s) 119, 149, 628, 658, 2302, 2332, 3213, 3243
Bsc4I CCNNNNNNNGG 6 cut(s) 578, 839, 1300, 1480, 1674, 2381
Bse118I RCCGGY 2 cut(s) 676, 2261
Bse1I ACTGG 8 cut(s) 294, 599, 611, 844, 1589, 2163, 2338, 2689
BseBI CCWGG 3 cut(s) 949, 1558, 1578
BseCI ATCGAT 1 cut(s) 1942
BseDI CCNNGG 6 cut(s) 183, 521, 1557, 1577, 1872, 1995
BseGI GGATG 9 cut(s) 728, 732, 820, 1348, 2047, 2132, 2569, 2934, 3089
BseLI CCNNNNNNNGG 6 cut(s) 578, 839, 1300, 1480, 1674, 2381
BseMII CTCAG 4 cut(s) 1807, 2081, 3198, 3213
BseNI ACTGG 8 cut(s) 294, 599, 611, 844, 1589, 2163, 2338, 2689
BseRI GAGGAG 6 cut(s) 65, 68, 140, 561, 636, 924
BseSI GKGCMC 2 cut(s) 1584, 1592
BsgI GTGCAG 3 cut(s) 888, 2934, 2985
Bsh1236I CGCG 2 cut(s) 1200, 1703
BshFI GGCC 5 cut(s) 526, 1313, 1994, 2342, 2667
BshNI GGYRCC 2 cut(s) 2258, 2812
BshVI ATCGAT 1 cut(s) 1942
BsiHKAI GWGCWC 4 cut(s) 586, 658, 1592, 3250
BsiSI CCGG 5 cut(s) 174, 677, 767, 2262, 2805
BslFI GGGAC 3 cut(s) 164, 1651, 2390
BslI CCNNNNNNNGG 6 cut(s) 578, 839, 1300, 1480, 1674, 2381
BsmAI GTCTC 3 cut(s) 775, 2496, 2870
BsmFI GGGAC 3 cut(s) 164, 1651, 2390
BsmI GAATGC 5 cut(s) 715, 1340, 2265, 2916, 2918
BsnI GGCC 5 cut(s) 526, 1313, 1994, 2342, 2667
Bso31I GGTCTC 1 cut(s) 775
Bsp1286I GDGCHC 7 cut(s) 586, 658, 1556, 1584, 1592, 3198, 3250
Bsp1407I TGTACA 4 cut(s) 73, 333, 983, 1602
Bsp19I CCATGG 1 cut(s) 1995
BspANI GGCC 5 cut(s) 526, 1313, 1994, 2342, 2667
BspCNI CTCAG 4 cut(s) 1806, 2080, 3199, 3212
BspDI ATCGAT 1 cut(s) 1942
BspFNI CGCG 2 cut(s) 1200, 1703
BspHI TCATGA 2 cut(s) 874, 1444
BspLI GGNNCC 7 cut(s) 1000, 1555, 2001, 2260, 2291, 2814, 3144
BspMAI CTGCAG 2 cut(s) 697, 3141
BspOI GCTAGC 1 cut(s) 3122
BspPI GGATC 9 cut(s) 28, 993, 1006, 1416, 1914, 2081, 2168, 2210, 3184
BspT107I GGYRCC 2 cut(s) 2258, 2812
BspTI CTTAAG 2 cut(s) 1924, 2708
BspTNI GGTCTC 1 cut(s) 775
BsrBI CCGCTC 1 cut(s) 620
BsrFI RCCGGY 2 cut(s) 676, 2261
BsrGI TGTACA 4 cut(s) 73, 333, 983, 1602
BsrI ACTGG 8 cut(s) 294, 599, 611, 844, 1589, 2163, 2338, 2689
BssAI RCCGGY 2 cut(s) 676, 2261
BssECI CCNNGG 6 cut(s) 183, 521, 1557, 1577, 1872, 1995
BssNI GRCGYC 1 cut(s) 2813
BssSI CACGAG 2 cut(s) 562, 585
BssT1I CCWWGG 3 cut(s) 183, 521, 1995
Bst2BI CACGAG 2 cut(s) 562, 585
Bst2UI CCWGG 3 cut(s) 949, 1558, 1578
Bst4CI ACNGT 8 cut(s) 1006, 1319, 1607, 2186, 2417, 2925, 3109, 3229
Bst6I CTCTTC 2 cut(s) 612, 2768
BstACI GRCGYC 1 cut(s) 2813
BstAFI CTTAAG 2 cut(s) 1924, 2708
BstAUI TGTACA 4 cut(s) 73, 333, 983, 1602
BstBAI YACGTR 1 cut(s) 2766
BstC8I GCNNGC 8 cut(s) 467, 678, 896, 2047, 2263, 2802, 2949, 3120
BstDEI CTNAG 6 cut(s) 11, 1793, 1916, 2067, 3199, 3207
BstDSI CCRYGG 2 cut(s) 1872, 1995
BstF5I GGATG 9 cut(s) 728, 732, 820, 1348, 2047, 2132, 2569, 2934, 3089
BstFNI CGCG 2 cut(s) 1200, 1703
BstH2I RGCGCY 2 cut(s) 254, 2816
BstHHI GCGC 3 cut(s) 253, 1705, 2815
BstMAI GTCTC 3 cut(s) 775, 2496, 2870
BstNI CCWGG 3 cut(s) 949, 1558, 1578
BstNSI RCATGY 3 cut(s) 944, 1380, 2478
BstSCI CCNGG 4 cut(s) 173, 947, 1556, 1576
BstSFI CTRYAG 4 cut(s) 693, 1057, 1669, 3137
BstSLI GKGCMC 2 cut(s) 1584, 1592
BstUI CGCG 2 cut(s) 1200, 1703
BstV2I GAAGAC 1 cut(s) 2122
BstX2I RGATCY 6 cut(s) 213, 998, 1408, 1919, 2215, 3176
BstXI CCANNNNNNTGG 1 cut(s) 1584
BstYI RGATCY 6 cut(s) 213, 998, 1408, 1919, 2215, 3176
Bsu15I ATCGAT 1 cut(s) 1942
BsuI GTATCC 1 cut(s) 2755
BsuRI GGCC 5 cut(s) 526, 1313, 1994, 2342, 2667
BsuTUI ATCGAT 1 cut(s) 1942
BtgI CCRYGG 2 cut(s) 1872, 1995
BtgZI GCGATG 1 cut(s) 1684
BtsCI GGATG 9 cut(s) 728, 732, 820, 1348, 2047, 2132, 2569, 2934, 3089
BtsI GCAGTG 2 cut(s) 2323, 2343
Cac8I GCNNGC 8 cut(s) 467, 678, 896, 2047, 2263, 2802, 2949, 3120
CaiI CAGNNNCTG 3 cut(s) 2732, 3206, 3233
CciI TCATGA 2 cut(s) 874, 1444
CfoI GCGC 3 cut(s) 253, 1705, 2815
Cfr10I RCCGGY 2 cut(s) 676, 2261
Cfr13I GGNCC 5 cut(s) 556, 665, 1312, 1665, 2290
ClaI ATCGAT 1 cut(s) 1942
CseI GACGC 1 cut(s) 3212
Csp6I GTAC 8 cut(s) 74, 118, 166, 334, 984, 1603, 2942, 3076
CviQI GTAC 8 cut(s) 74, 118, 166, 334, 984, 1603, 2942, 3076
DdeI CTNAG 6 cut(s) 11, 1793, 1916, 2067, 3199, 3207
DinI GGCGCC 1 cut(s) 2814
DraI TTTAAA 2 cut(s) 492, 816
DraIII CACNNNGTG 1 cut(s) 662
EaeI YGGCCR 1 cut(s) 1992
Eam1104I CTCTTC 2 cut(s) 612, 2768
EarI CTCTTC 2 cut(s) 612, 2768
EciI GGCGGA 1 cut(s) 2496
Ecl136II GAGCTC 1 cut(s) 584
Eco130I CCWWGG 3 cut(s) 183, 521, 1995
Eco24I GRGCYC 3 cut(s) 586, 1556, 3198
Eco31I GGTCTC 1 cut(s) 775
Eco47I GGWCC 4 cut(s) 556, 665, 1665, 2290
Eco47III AGCGCT 1 cut(s) 252
Eco53kI GAGCTC 1 cut(s) 584
Eco57I CTGAAG 2 cut(s) 355, 461
EcoICRI GAGCTC 1 cut(s) 584
EcoO109I RGGNCCY 1 cut(s) 1665
EcoRII CCWGG 3 cut(s) 947, 1556, 1576
EcoT14I CCWWGG 3 cut(s) 183, 521, 1995
EcoT22I ATGCAT 2 cut(s) 2134, 2918
EcoT38I GRGCYC 3 cut(s) 586, 1556, 3198
EgeI GGCGCC 1 cut(s) 2814
EheI GGCGCC 1 cut(s) 2814
ErhI CCWWGG 3 cut(s) 183, 521, 1995
FalI AAGNNNNNCTT 2 cut(s) 203, 235
FaqI GGGAC 3 cut(s) 164, 1651, 2390
FauI CCCGC 3 cut(s) 681, 2992, 3123
FbaI TGATCA 3 cut(s) 477, 871, 2848
FokI GGATG 9 cut(s) 735, 739, 807, 1335, 2054, 2119, 2556, 2941, 3076
FriOI GRGCYC 3 cut(s) 586, 1556, 3198
FspBI CTAG 8 cut(s) 417, 956, 1641, 2349, 2561, 2885, 2990, 3119
GlaI GCGC 3 cut(s) 252, 1704, 2814
GsuI CTGGAG 3 cut(s) 582, 1540, 1560
HaeII RGCGCY 2 cut(s) 254, 2816
HaeIII GGCC 5 cut(s) 526, 1313, 1994, 2342, 2667
HapII CCGG 5 cut(s) 174, 677, 767, 2262, 2805
HgaI GACGC 1 cut(s) 3212
HhaI GCGC 3 cut(s) 253, 1705, 2815
Hin1I GRCGYC 1 cut(s) 2813
Hin6I GCGC 3 cut(s) 251, 1703, 2813
HinP1I GCGC 3 cut(s) 251, 1703, 2813
HincII GTYRAC 5 cut(s) 357, 1195, 1564, 2704, 2987
HindII GTYRAC 5 cut(s) 357, 1195, 1564, 2704, 2987
HindIII AAGCTT 2 cut(s) 1424, 1530
HpaI GTTAAC 2 cut(s) 1195, 2704
HpaII CCGG 5 cut(s) 174, 677, 767, 2262, 2805
HphI GGTGA 7 cut(s) 172, 304, 413, 771, 1754, 2529, 2834
Hpy99I CGWCG 1 cut(s) 319
HpyCH4III ACNGT 8 cut(s) 1006, 1319, 1607, 2186, 2417, 2925, 3109, 3229
HpyCH4IV ACGT 4 cut(s) 317, 2149, 2765, 2960
HpyF3I CTNAG 6 cut(s) 11, 1793, 1916, 2067, 3199, 3207
HpySE526I ACGT 4 cut(s) 317, 2149, 2765, 2960
Hsp92I GRCGYC 1 cut(s) 2813
HspAI GCGC 3 cut(s) 251, 1703, 2813
KasI GGCGCC 1 cut(s) 2812
KroI GCCGGC 2 cut(s) 676, 2261
KroNI GCCGGC 2 cut(s) 678, 2263
Ksp22I TGATCA 3 cut(s) 477, 871, 2848
KspAI GTTAAC 2 cut(s) 1195, 2704
LmnI GCTCC 9 cut(s) 19, 581, 649, 653, 769, 1559, 1579, 3142, 3245
MaeI CTAG 8 cut(s) 417, 956, 1641, 2349, 2561, 2885, 2990, 3119
MaeII ACGT 4 cut(s) 317, 2149, 2765, 2960
MaeIII GTNAC 8 cut(s) 601, 1072, 1092, 1372, 1867, 2535, 2822, 3223
MbiI CCGCTC 1 cut(s) 620
MfeI CAATTG 3 cut(s) 1361, 1932, 2595
MflI RGATCY 6 cut(s) 213, 998, 1408, 1919, 2215, 3176
MhlI GDGCHC 7 cut(s) 586, 658, 1556, 1584, 1592, 3198, 3250
Mly113I GGCGCC 1 cut(s) 2813
MlyI GAGTC 6 cut(s) 101, 352, 589, 1219, 2450, 2872
MmeI TCCRAC 3 cut(s) 1175, 2354, 3142
Mph1103I ATGCAT 2 cut(s) 2134, 2918
MroNI GCCGGC 2 cut(s) 676, 2261
MroXI GAANNNNTTC 5 cut(s) 119, 215, 1427, 2427, 2916
MslI CAYNNNNRTG 6 cut(s) 185, 1007, 1263, 1595, 2831, 3071
MspA1I CMGCKG 1 cut(s) 698
MspCI CTTAAG 2 cut(s) 1924, 2708
MspI CCGG 5 cut(s) 174, 677, 767, 2262, 2805
MspR9I CCNGG 4 cut(s) 175, 949, 1558, 1578
MunI CAATTG 3 cut(s) 1361, 1932, 2595
Mva1269I GAATGC 5 cut(s) 715, 1340, 2265, 2916, 2918
MvaI CCWGG 3 cut(s) 949, 1558, 1578
MvnI CGCG 2 cut(s) 1200, 1703
NaeI GCCGGC 2 cut(s) 678, 2263
NarI GGCGCC 1 cut(s) 2813
NciI CCSGG 1 cut(s) 175
NcoI CCATGG 1 cut(s) 1995
NgoMIV GCCGGC 2 cut(s) 676, 2261
NheI GCTAGC 1 cut(s) 3118
NlaIV GGNNCC 7 cut(s) 1000, 1555, 2001, 2260, 2291, 2814, 3144
NmuCI GTSAC 2 cut(s) 2535, 2822
NsiI ATGCAT 2 cut(s) 2134, 2918
NspI RCATGY 3 cut(s) 944, 1380, 2478
OliI CACNNNNGTG 2 cut(s) 185, 2831
PagI TCATGA 2 cut(s) 874, 1444
PciI ACATGT 1 cut(s) 1376
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PctI GAATGC 5 cut(s) 715, 1340, 2265, 2916, 2918
PdiI GCCGGC 2 cut(s) 678, 2263
PdmI GAANNNNTTC 5 cut(s) 119, 215, 1427, 2427, 2916
PfeI GAWTC 9 cut(s) 99, 196, 1178, 1839, 1855, 1887, 2071, 2167, 2609
PflMI CCANNNNNTGG 2 cut(s) 839, 1300
PleI GAGTC 6 cut(s) 100, 352, 589, 1218, 2450, 2872
PluTI GGCGCC 1 cut(s) 2816
PpsI GAGTC 6 cut(s) 100, 352, 589, 1218, 2450, 2872
Ppu21I YACGTR 1 cut(s) 2766
PpuMI RGGWCCY 1 cut(s) 1665
PscI ACATGT 1 cut(s) 1376
PshBI ATTAAT 1 cut(s) 2022
Psp124BI GAGCTC 1 cut(s) 586
Psp1406I AACGTT 1 cut(s) 2149
Psp5II RGGWCCY 1 cut(s) 1665
Psp6I CCWGG 3 cut(s) 947, 1556, 1576
PspGI CCWGG 3 cut(s) 947, 1556, 1576
PspN4I GGNNCC 7 cut(s) 1000, 1555, 2001, 2260, 2291, 2814, 3144
PspPI GGNCC 5 cut(s) 556, 665, 1312, 1665, 2290
PspPPI RGGWCCY 1 cut(s) 1665
PstI CTGCAG 2 cut(s) 697, 3141
PstNI CAGNNNCTG 3 cut(s) 2732, 3206, 3233
PsuI RGATCY 6 cut(s) 213, 998, 1408, 1919, 2215, 3176
PvuII CAGCTG 1 cut(s) 698
RsaI GTAC 8 cut(s) 75, 119, 167, 335, 985, 1604, 2943, 3077
RsaNI GTAC 8 cut(s) 74, 118, 166, 334, 984, 1603, 2942, 3076
RseI CAYNNNNRTG 6 cut(s) 185, 1007, 1263, 1595, 2831, 3071
SacI GAGCTC 1 cut(s) 586
Sau96I GGNCC 5 cut(s) 556, 665, 1312, 1665, 2290
ScaI AGTACT 1 cut(s) 119
SchI GAGTC 6 cut(s) 101, 352, 589, 1219, 2450, 2872
ScrFI CCNGG 4 cut(s) 175, 949, 1558, 1578
SduI GDGCHC 7 cut(s) 586, 658, 1556, 1584, 1592, 3198, 3250
SfcI CTRYAG 4 cut(s) 693, 1057, 1669, 3137
SfoI GGCGCC 1 cut(s) 2814
SinI GGWCC 4 cut(s) 556, 665, 1665, 2290
SmiMI CAYNNNNRTG 6 cut(s) 185, 1007, 1263, 1595, 2831, 3071
SmlI CTYRAG 5 cut(s) 47, 83, 1834, 1924, 2708
SmoI CTYRAG 5 cut(s) 47, 83, 1834, 1924, 2708
SpeI ACTAGT 2 cut(s) 1640, 2989
SspDI GGCGCC 1 cut(s) 2812
SspI AATATT 2 cut(s) 1489, 3019
SspMI CTAG 8 cut(s) 417, 956, 1641, 2349, 2561, 2885, 2990, 3119
SstI GAGCTC 1 cut(s) 586
StyD4I CCNGG 4 cut(s) 173, 947, 1556, 1576
StyI CCWWGG 3 cut(s) 183, 521, 1995
TaaI ACNGT 8 cut(s) 1006, 1319, 1607, 2186, 2417, 2925, 3109, 3229
TaiI ACGT 4 cut(s) 320, 2152, 2768, 2963
TatI WGTACW 7 cut(s) 73, 117, 333, 983, 1602, 2941, 3075
TauI GCSGC 3 cut(s) 620, 1166, 2935
TfiI GAWTC 9 cut(s) 99, 196, 1178, 1839, 1855, 1887, 2071, 2167, 2609
TseFI GTSAC 2 cut(s) 2535, 2822
Tsp45I GTSAC 2 cut(s) 2535, 2822
TspGWI ACGGA 2 cut(s) 732, 3258
Van91I CCANNNNNTGG 2 cut(s) 839, 1300
Vha464I CTTAAG 2 cut(s) 1924, 2708
VneI GTGCAC 1 cut(s) 1588
VpaK11BI GGWCC 4 cut(s) 556, 665, 1665, 2290
VspI ATTAAT 1 cut(s) 2022
XapI RAATTY 3 cut(s) 59, 1760, 2251
XceI RCATGY 3 cut(s) 944, 1380, 2478
XcmI CCANNNNNNNNNTGG 1 cut(s) 1125
XmnI GAANNNNTTC 5 cut(s) 119, 215, 1427, 2427, 2916
XspI CTAG 8 cut(s) 417, 956, 1641, 2349, 2561, 2885, 2990, 3119
ZrmI AGTACT 1 cut(s) 119
Zsp2I ATGCAT 2 cut(s) 2134, 2918
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.