Rroxscaffold_5G00365920

Topless-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0013792
Physical Location & Seq
Forward (+)
47637066 .. 47644748
7683 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00365920.1

Sequence Viewer

Length: 3375 bp
ATGTCATCTCTGAGCAGAGAGCTGGTTTTCCTGATCCTTCAATTTCTTGAGGAGGAGAAATTCAAGGAGGCTGTACATAGGCTTGAGCAAGAGTCTGGATTCTTCTTCAACATGAAGTACTTTGAGGAGAAAGCGTTGCAGGGAGAGTGGGACGAGGTGGAGAAGTACCTGTCCGGGTTCACCAAGGTGGACGAGAATCGCTACTCTATGAAGATCTTCTTCGAGGTCAGAAAGCAGAAGTATCTCGAAGCTCTGGACAGGCAAGACAGGGCGAAAGCCGTTGAGATACTGGTGAAGGATTTGAAGGTGTTTTCGACGTTTAATGAGGACTTGTACAAGGAAATCACTCATCTGTTGACTCTTGACAACTTCAGAGAAAATGAGCAGTTGTCGAAGTATGGTGATACTAAATCCGCTAGAAGTATAATGCTGGTAGAGCTGAAGAAATTGATAGAAGCAAATCCTCTATTCCGAGAGAAGCTGGTTTTCCCCACTTTGAAGGCGTCACGCTTGCGAACTTTGATCAATCAAAGTTTAAATTGGCAGCACCAGCTATGCAAAAACCCAAGGCCAAATCCTGACATCAAGACCTTATTCATGGACCACTCGTGTTCTCCTCCAAATGGAGCTCGTGCATCGACTCCAGTTACTCTTCCAGTTGCAGCTCTTACAAAGCCTTTAACTTATGCTCCTCTTGGAGCACATGGTGGACCTTTCCCGCCGGCTGCTGCTGCTGCAGCTGCCAATGCGAATGCTTTAGCAGGATGGATGTCCAATGCTAATCCTTCCTTGTCTATGCAATCAGCTCCGGTCTCTGCTTCACCTCTGCCTGTTCAACTTGGTCAAGTTTCTTCTTTAAAGCATCCAAGAACACCATCAACTGGTCTTGGAATGATTGATTATCAAAGCTCTGATCATGAGCAACTAATGAAACGCCTGCGGACTGCACAATCTGTTGATGAGGTTAGCTATCCTCCTCAACATGCTTCCTGGTCGCTAGATGACCTACCGAGAAATGTAGTTTGTACACTTCGTCAGGGATCCATTGTTATGAGCATGGATTTCCACCCTTCTCATCACACATTACTTGCTGTTGGCTGTACTAATGGAGAAGTTACTCTGTGGGAAGTTGGGTTACGAGAGAGGTTGGTGTCCAAGCCATTCAAAGTATGGGATTTGGCTGCTTGTTCTCTGTCATTTCAGGCGGCTATTGTCAAAGATTCATCAATGTCTGTTAACCGTGTATCATGGAGTCCAGACGGGAACTTAATCGGTGTTGCATTTACGAAACATTTGGTTCATCTGTATTCTTATCAAGGGTCTAATGATCTACGCCAACTTTTGGAGATTGATGCCCACAGTGGAGGTGTGAATGACTTGGCATTCTCTCATCCAAACAAGCAATTATGTGTTGTAACATGTGGAGATGATAAGCTGATAAAGGTTTGGGATCTGTCTGGAAGGAAGCTTTTCCACTTTGAAGGTCATGAAGCACCTGTTTACTCGGTTTGCCCCCACCAGAAGGAGAATATTCAGTTTATATTTTCAACTGCTGTTGATGGAAAAATAAAAGCTTGGCTGTATGACAATGTGGGCTCCAGGGTTGACTATGATGCTCCAGGGCAGTGGTGCACCACAATGCTGTACAGTGATGATGGAAATAGATTGTTCTCTTGTGGGACTAGTAAAGAGGGTGACTCCTTCCTGGTTGAATGGAATGAAAGTGAAGGGGCAATAAAGAGGACCTATAGTGGGTTCAGAAAGAAATCTAATGGCATCGCACAATTTGATACCACAAGAAATCACTTTTTGGCTGTTGGTGAAGATAGCTCGATAAAATTTTGGGATATGGATAATACAAATGTTCTTCTCAGTATAGAGGCTGATGGTGGACTACCACCTGTTCCTCGCTTGAGATTCAATAAGGAAGGGAATCTGCTTGTTGTTACCACGGCTGACAATGGATTCAAGGTTCTTGCAAATGCTGATGGTCTCAGATCCTTAAGAGCAATTGAAAATCGATCTTATGAAGCATCAAGAACACCTATTGAGATGAAGGTATCTACGTCGGCCATGGTTCCTAATATCAATCCTGCCATTAATAAAGTAGATCGTATGGATGCAAGCTCTCCTGCCAGACCTACTCAGATTCTTAATGGAGTTGATCCTATGGCAAGAAGTGTAGAAAAGCGAAGACTATTAGATGATGCATCCGAGAAAAATAAACGTTGGGAACTGGCAGAGATTGTTGATCCTGTTCACTGTCGAGTGGCTACGATGCCAGATAGTAAGGATCCTGCTAACAAGGTTGCTCGACTTCTTTACACAAATTCTGGTGCCGGCATTCTGGCTCTTGGTTCAAATGGGGTCCAGAAGCTATGGAAGTGGAGAAGCAGTGATCAAAATCCAAGTGGAAAGGCCACTGCTAGTGTCGTTCCACAACATTGGCAACCCAACAATGGTCTTTTGATGACTAATGATGTCCCTGAAAACTGTGAGGAAGCAGTTCCATGTATTGCACTCTCAAAGAATGACTCCTATGTGATGTCGGCATGTGGCGGAAAAGTGTCTCTGTTCAATATGATGACATTTAAGGTAATGACAACTTTCATGGCACCTCCACCAGCCTCAACCTTCTTAGCATTTCATCCTCTGGATAATAACATCATAGCAATTGGAACAGAAGATTCAACTATCCACATTTACAATGTTAGAGTTGATGAGGTCAAATCCAAACTGAAAGGCCACCAGAAACACATTACTGGATTAGCGTTTTCTGTTAACCTTAAGATCATGGTTTCATCAGGTGCTGATGCTCAACTATGCTTTTGGAACATGGATACGTGGGAGAAGAGGAAGTCGCTGACAATTCAACTGCCAGCTGGAAAGGCGCCTGTTGGTGACACACAAGTGCAATTCTATTCTGATCAAACTCGGTTGTTGGTATGCCATGAGACTCAACTAGCACTATATGATGCTGCCAAAGCAGAATGCATTCGACAGTGGATGCCGCAAGATGTACTTCCTGCACCAATAACATGTGCAGCATATTCCCCCAACAGTCAACTAGTCTATGCGGGTTTTACTGATGGCAATATTGGAGTATTTGATGCTGATAGTCTGAAATTAAGATGTCGTATTTCCATGTCAGTGTACTTGGCACAAGCATCCCCAAGCAGCCAAACTGTGTACCCGCTAGCTCTTACAGCACATCTGCAGGAGCCATATCAGTTCGCCGTTGGACTGACAGATGGATCTGTTAAAGTTATAGAGCCCTCAGACGCTGAGGGGAAGTGGGGAGTTACAGTGCCTGTCGATAACGGAGCACAGAACGGCTGGACAGCAACATCTTCGTCTAACAATCCTGCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

1124

Amino Acids

124.5

Weight (kDa)

6.55

Isoelectric Point (pI)

43.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH_TPL PF17814 5 - 34 3.6e-11 LisH-like dimerisation domain
TPR1-like_2nd PF21889 37 - 161 9.4e-62 TPR1-like, CTLH-containing domain
zf_topless PF21359 164 - 204 2.8e-25 TOPLESS, zinc finger domain
Beta-prop_EML_2 PF23414 330 - 467 8.3e-08 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd PF25172 349 - 563 6.3e-10 WDR3 second beta-propeller domain
Beta-prop_RIG_2nd PF23775 358 - 499 5.3e-06 RIG second beta-propeller
Beta-prop_WDR3_1st PF25173 389 - 561 1.3e-14 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 390 - 491 2.5e-09 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 402 - 560 3.1e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 407 - 623 3.9e-21 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 410 - 662 2.7e-20 THOC3 beta-propeller domain
WDR55 PF24796 411 - 526 1.1e-08 WDR55
Beta-prop_EML PF23409 446 - 652 9.1e-08 Echinoderm microtubule-associated protein first beta-propeller
WD40 PF00400 447 - 484 1.8e-06 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 457 - 529 7.6e-08 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 464 - 560 5.4e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 520 - 658 3.2e-06 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 816 - 956 3.9e-14 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 816 - 1049 2.2e-13 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 830 - 955 3.3e-16 WDR3 first beta-propeller domain
WDR55 PF24796 830 - 947 1.4e-08 WDR55
Beta-prop_EML_2 PF23414 831 - 954 1.5e-12 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 831 - 956 1.3e-12 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 832 - 1040 2.2e-21 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 832 - 1042 9.1e-18 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 833 - 948 1.1e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 836 - 954 1.3e-13 WDR3 second beta-propeller domain
Beta-prop_IFT140_1st PF23383 849 - 948 7.8e-09 IFT140 first beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 853 - 977 1.3e-11 WDR36/Utp21 first beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 855 - 948 3e-08 WDR90/POC16, second beta-propeller
Beta-prop_RIG_1st PF23770 863 - 926 9.9e-06 RIG first beta-propeller
WD40_Gbeta PF25391 884 - 1038 1.3e-06 G protein beta WD-40 repeat protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013792)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G16830
fragaria_vesca FvH4_4g18910 FvH4_4g18910
malus_domestica MD13G1162100.v1.1 MD16G1161900.v1.1
prunus_persica Prupe.1G129400_v2.0.a1
pyrus_communis pycom13g13830
rosa_chinensis RchiOBHm_Chr4g0423811
rosa_laevigata RLG00000007530
rosa_multiflora Rmu_sc0003369.1_g000018
rosa_roxburghii Rroxscaffold_5G00365920
rosa_rugosa Rorug04G0190900
rosa_samantha Rh4AG250300 Rh4BG255600 Rh4CG263700 Rh4DG250000
rosa_wichuraiana Rw4G021430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 2336, 2614, 2890
AccB7I CCANNNNNTGG 2 cut(s) 881, 1342
AciI CCGC 8 cut(s) 414, 719, 940, 1205, 2559, 3011, 3077, 3194
AclI AACGTT 1 cut(s) 2227
AcoI YGGCCR 1 cut(s) 2070
AcsI RAATTY 3 cut(s) 59, 1838, 2329
AcuI CTGAAG 2 cut(s) 355, 461
AcyI GRCGYC 2 cut(s) 503, 2891
AdeI CACNNNGTG 1 cut(s) 707
AfiI CCNNNNNNNGG 6 cut(s) 623, 881, 1342, 1522, 1752, 2459
AflII CTTAAG 2 cut(s) 2002, 2786
AflIII ACRYGT 2 cut(s) 1418, 3038
AhlI ACTAGT 2 cut(s) 1682, 3067
AjnI CCWGG 4 cut(s) 989, 1598, 1618, 1704
AleI CACNNNNGTG 2 cut(s) 185, 2909
AloI GAACNNNNNNTCC 2 cut(s) 1956, 1988
Alw21I GWGCWC 4 cut(s) 631, 703, 1634, 3328
Alw26I GTCTC 4 cut(s) 817, 1997, 2574, 2948
Alw44I GTGCAC 1 cut(s) 1630
AlwNI CAGNNNCTG 3 cut(s) 2810, 3284, 3311
AoxI GGCC 4 cut(s) 569, 2070, 2418, 2743
ApaLI GTGCAC 1 cut(s) 1630
ApoI RAATTY 3 cut(s) 59, 1838, 2329
AseI ATTAAT 1 cut(s) 2100
Asp700I GAANNNNTTC 4 cut(s) 215, 1469, 2505, 2994
AspLEI GCGC 1 cut(s) 2893
AspS9I GGNCC 4 cut(s) 601, 710, 1743, 2368
AsuC2I CCSGG 1 cut(s) 175
AsuHPI GGTGA 7 cut(s) 172, 304, 413, 813, 1706, 1832, 2912
AsuNHI GCTAGC 1 cut(s) 3196
AvaII GGWCC 4 cut(s) 601, 710, 1743, 2368
BaeGI GKGCMC 1 cut(s) 1634
BamHI GGATCC 2 cut(s) 1040, 2293
BanI GGYRCC 3 cut(s) 2336, 2614, 2890
BanII GRGCYC 3 cut(s) 631, 1598, 3276
BarI GAAGNNNNNNTAC 2 cut(s) 1119, 1151
BauI CACGAG 2 cut(s) 607, 630
BbsI GAAGAC 1 cut(s) 2200
Bbv12I GWGCWC 4 cut(s) 631, 703, 1634, 3328
BbvCI CCTCAGC 1 cut(s) 3285
BccI CCATC 8 cut(s) 759, 883, 1553, 1649, 1880, 1982, 3083, 3245
BceAI ACGGC 4 cut(s) 263, 1968, 3221, 3349
BcgI CGANNNNNNTGC 2 cut(s) 3333, 3367
BciT130I CCWGG 4 cut(s) 991, 1600, 1620, 1706
BciVI GTATCC 1 cut(s) 2833
BclI TGATCA 4 cut(s) 522, 913, 2398, 2926
BcnI CCSGG 1 cut(s) 175
BcoDI GTCTC 4 cut(s) 817, 1997, 2574, 2948
BcuI ACTAGT 2 cut(s) 1682, 3067
BfaI CTAG 7 cut(s) 417, 998, 1683, 2427, 2963, 3068, 3197
BfmI CTRYAG 3 cut(s) 735, 1747, 3215
BfoI RGCGCY 1 cut(s) 2894
BfrI CTTAAG 2 cut(s) 2002, 2786
BfuI GTATCC 1 cut(s) 2833
BglII AGATCT 1 cut(s) 213
BmcAI AGTACT 1 cut(s) 119
Bme1390I CCNGG 5 cut(s) 175, 991, 1600, 1620, 1706
Bme18I GGWCC 4 cut(s) 601, 710, 1743, 2368
BmgT120I GGNCC 4 cut(s) 601, 710, 1743, 2368
BmiI GGNNCC 9 cut(s) 1042, 1597, 2079, 2295, 2338, 2369, 2616, 2892, 3222
BmrFI CCNGG 5 cut(s) 175, 991, 1600, 1620, 1706
BmtI GCTAGC 1 cut(s) 3200
BpiI GAAGAC 1 cut(s) 2200
BplI GAGNNNNNCTC 2 cut(s) 1682, 1714
BpmI CTGGAG 3 cut(s) 627, 1582, 1602
Bpu10I CCTNAGC 1 cut(s) 3285
BpuEI CTTGAG 3 cut(s) 68, 104, 1933
BpuMI CCSGG 1 cut(s) 175
Bsa29I ATCGAT 1 cut(s) 2020
BsaAI YACGTR 1 cut(s) 2844
BsaBI GATNNNNATC 1 cut(s) 2403
BsaHI GRCGYC 2 cut(s) 503, 2891
BsaI GGTCTC 2 cut(s) 817, 1997
BsaJI CCNNGG 6 cut(s) 183, 566, 1599, 1619, 1950, 2073
BsaWI WCCGGW 1 cut(s) 808
BsaXI ACNNNNNCTCC 8 cut(s) 119, 149, 673, 703, 2380, 2410, 3291, 3321
Bsc4I CCNNNNNNNGG 6 cut(s) 623, 881, 1342, 1522, 1752, 2459
Bse118I RCCGGY 2 cut(s) 721, 2339
Bse1I ACTGG 6 cut(s) 294, 644, 656, 886, 2241, 2767
Bse8I GATNNNNATC 1 cut(s) 2403
BseBI CCWGG 4 cut(s) 991, 1600, 1620, 1706
BseCI ATCGAT 1 cut(s) 2020
BseDI CCNNGG 6 cut(s) 183, 566, 1599, 1619, 1950, 2073
BseGI GGATG 9 cut(s) 770, 774, 862, 1390, 2125, 2210, 2647, 3012, 3167
BseJI GATNNNNATC 1 cut(s) 2403
BseLI CCNNNNNNNGG 6 cut(s) 623, 881, 1342, 1522, 1752, 2459
BseMII CTCAG 5 cut(s) 1885, 2008, 2159, 3276, 3291
BseNI ACTGG 6 cut(s) 294, 644, 656, 886, 2241, 2767
BseRI GAGGAG 6 cut(s) 65, 68, 140, 606, 681, 966
BseSI GKGCMC 1 cut(s) 1634
BsgI GTGCAG 3 cut(s) 930, 3012, 3063
BshFI GGCC 4 cut(s) 571, 2072, 2420, 2745
BshNI GGYRCC 3 cut(s) 2336, 2614, 2890
BshVI ATCGAT 1 cut(s) 2020
BsiHKAI GWGCWC 4 cut(s) 631, 703, 1634, 3328
BsiSI CCGG 4 cut(s) 174, 722, 809, 2340
BslFI GGGAC 3 cut(s) 164, 1693, 2468
BslI CCNNNNNNNGG 6 cut(s) 623, 881, 1342, 1522, 1752, 2459
BsmAI GTCTC 4 cut(s) 817, 1997, 2574, 2948
BsmFI GGGAC 3 cut(s) 164, 1693, 2468
BsmI GAATGC 5 cut(s) 757, 1382, 2343, 2994, 2996
BsnI GGCC 4 cut(s) 571, 2072, 2420, 2745
Bso31I GGTCTC 2 cut(s) 817, 1997
Bsp1286I GDGCHC 6 cut(s) 631, 703, 1598, 1634, 3276, 3328
Bsp1407I TGTACA 4 cut(s) 73, 333, 1025, 1644
Bsp19I CCATGG 1 cut(s) 2073
BspACI CCGC 8 cut(s) 414, 719, 940, 1205, 2559, 3011, 3077, 3194
BspANI GGCC 4 cut(s) 571, 2072, 2420, 2745
BspCNI CTCAG 5 cut(s) 1884, 2007, 2158, 3277, 3290
BspDI ATCGAT 1 cut(s) 2020
BspHI TCATGA 2 cut(s) 916, 1486
BspLI GGNNCC 9 cut(s) 1042, 1597, 2079, 2295, 2338, 2369, 2616, 2892, 3222
BspMAI CTGCAG 2 cut(s) 739, 3219
BspOI GCTAGC 1 cut(s) 3200
BspT107I GGYRCC 3 cut(s) 2336, 2614, 2890
BspTI CTTAAG 2 cut(s) 2002, 2786
BspTNI GGTCTC 2 cut(s) 817, 1997
BsrFI RCCGGY 2 cut(s) 721, 2339
BsrGI TGTACA 4 cut(s) 73, 333, 1025, 1644
BsrI ACTGG 6 cut(s) 294, 644, 656, 886, 2241, 2767
BssAI RCCGGY 2 cut(s) 721, 2339
BssECI CCNNGG 6 cut(s) 183, 566, 1599, 1619, 1950, 2073
BssNI GRCGYC 2 cut(s) 503, 2891
BssSI CACGAG 2 cut(s) 607, 630
BssT1I CCWWGG 3 cut(s) 183, 566, 2073
Bst2BI CACGAG 2 cut(s) 607, 630
Bst2UI CCWGG 4 cut(s) 991, 1600, 1620, 1706
Bst4CI ACNGT 9 cut(s) 1241, 1361, 1649, 2264, 2495, 3003, 3062, 3187, 3307
Bst6I CTCTTC 2 cut(s) 657, 2846
BstACI GRCGYC 2 cut(s) 503, 2891
BstAFI CTTAAG 2 cut(s) 2002, 2786
BstAUI TGTACA 4 cut(s) 73, 333, 1025, 1644
BstBAI YACGTR 1 cut(s) 2844
BstC8I GCNNGC 7 cut(s) 512, 723, 938, 2125, 2341, 2880, 3198
BstDEI CTNAG 7 cut(s) 11, 1871, 1994, 2145, 2638, 3277, 3285
BstDSI CCRYGG 2 cut(s) 1950, 2073
BstF5I GGATG 9 cut(s) 770, 774, 862, 1390, 2125, 2210, 2647, 3012, 3167
BstH2I RGCGCY 1 cut(s) 2894
BstHHI GCGC 1 cut(s) 2893
BstMAI GTCTC 4 cut(s) 817, 1997, 2574, 2948
BstNI CCWGG 4 cut(s) 991, 1600, 1620, 1706
BstNSI RCATGY 4 cut(s) 986, 1422, 2556, 3042
BstSCI CCNGG 5 cut(s) 173, 989, 1598, 1618, 1704
BstSFI CTRYAG 3 cut(s) 735, 1747, 3215
BstSLI GKGCMC 1 cut(s) 1634
BstV2I GAAGAC 1 cut(s) 2200
BstX2I RGATCY 6 cut(s) 213, 1040, 1450, 1997, 2293, 3254
BstXI CCANNNNNNTGG 2 cut(s) 1626, 2445
BstYI RGATCY 6 cut(s) 213, 1040, 1450, 1997, 2293, 3254
Bsu15I ATCGAT 1 cut(s) 2020
BsuI GTATCC 1 cut(s) 2833
BsuRI GGCC 4 cut(s) 571, 2072, 2420, 2745
BsuTUI ATCGAT 1 cut(s) 2020
BtgI CCRYGG 2 cut(s) 1950, 2073
BtgZI GCGATG 1 cut(s) 1762
BtsCI GGATG 9 cut(s) 770, 774, 862, 1390, 2125, 2210, 2647, 3012, 3167
BtsI GCAGTG 3 cut(s) 1631, 2401, 2421
BtsIMutI CAGTG 9 cut(s) 1366, 1631, 1654, 2260, 2401, 2421, 3008, 3156, 3312
Cac8I GCNNGC 7 cut(s) 512, 723, 938, 2125, 2341, 2880, 3198
CaiI CAGNNNCTG 3 cut(s) 2810, 3284, 3311
CciI TCATGA 2 cut(s) 916, 1486
CfoI GCGC 1 cut(s) 2893
Cfr10I RCCGGY 2 cut(s) 721, 2339
Cfr13I GGNCC 4 cut(s) 601, 710, 1743, 2368
ClaI ATCGAT 1 cut(s) 2020
CseI GACGC 2 cut(s) 492, 3290
DdeI CTNAG 7 cut(s) 11, 1871, 1994, 2145, 2638, 3277, 3285
DinI GGCGCC 1 cut(s) 2892
DraI TTTAAA 2 cut(s) 537, 858
DraIII CACNNNGTG 1 cut(s) 707
EaeI YGGCCR 1 cut(s) 2070
Eam1104I CTCTTC 2 cut(s) 657, 2846
EarI CTCTTC 2 cut(s) 657, 2846
EciI GGCGGA 1 cut(s) 2574
Ecl136II GAGCTC 1 cut(s) 629
Eco130I CCWWGG 3 cut(s) 183, 566, 2073
Eco24I GRGCYC 3 cut(s) 631, 1598, 3276
Eco31I GGTCTC 2 cut(s) 817, 1997
Eco47I GGWCC 4 cut(s) 601, 710, 1743, 2368
Eco53kI GAGCTC 1 cut(s) 629
Eco57I CTGAAG 2 cut(s) 355, 461
EcoICRI GAGCTC 1 cut(s) 629
EcoO109I RGGNCCY 1 cut(s) 1743
EcoRII CCWGG 4 cut(s) 989, 1598, 1618, 1704
EcoT14I CCWWGG 3 cut(s) 183, 566, 2073
EcoT22I ATGCAT 2 cut(s) 2212, 2996
EcoT38I GRGCYC 3 cut(s) 631, 1598, 3276
EgeI GGCGCC 1 cut(s) 2892
EheI GGCGCC 1 cut(s) 2892
ErhI CCWWGG 3 cut(s) 183, 566, 2073
FalI AAGNNNNNCTT 4 cut(s) 203, 235, 3006, 3038
FaqI GGGAC 3 cut(s) 164, 1693, 2468
FauI CCCGC 3 cut(s) 726, 3070, 3201
FbaI TGATCA 4 cut(s) 522, 913, 2398, 2926
FokI GGATG 9 cut(s) 777, 781, 849, 1377, 2132, 2197, 2634, 3019, 3154
FriOI GRGCYC 3 cut(s) 631, 1598, 3276
FspBI CTAG 7 cut(s) 417, 998, 1683, 2427, 2963, 3068, 3197
GlaI GCGC 1 cut(s) 2892
GsuI CTGGAG 3 cut(s) 627, 1582, 1602
HaeII RGCGCY 1 cut(s) 2894
HaeIII GGCC 4 cut(s) 571, 2072, 2420, 2745
HapII CCGG 4 cut(s) 174, 722, 809, 2340
HgaI GACGC 2 cut(s) 492, 3290
HhaI GCGC 1 cut(s) 2893
Hin1I GRCGYC 2 cut(s) 503, 2891
Hin6I GCGC 1 cut(s) 2891
HinP1I GCGC 1 cut(s) 2891
HincII GTYRAC 5 cut(s) 357, 1237, 1606, 2782, 3065
HindII GTYRAC 5 cut(s) 357, 1237, 1606, 2782, 3065
HindIII AAGCTT 2 cut(s) 1466, 1572
HpaI GTTAAC 2 cut(s) 1237, 2782
HpaII CCGG 4 cut(s) 174, 722, 809, 2340
HphI GGTGA 7 cut(s) 172, 304, 413, 813, 1706, 1832, 2912
Hpy99I CGWCG 2 cut(s) 319, 2071
HpyCH4III ACNGT 9 cut(s) 1241, 1361, 1649, 2264, 2495, 3003, 3062, 3187, 3307
HpyCH4IV ACGT 4 cut(s) 317, 2066, 2227, 2843
HpyF3I CTNAG 7 cut(s) 11, 1871, 1994, 2145, 2638, 3277, 3285
HpySE526I ACGT 4 cut(s) 317, 2066, 2227, 2843
Hsp92I GRCGYC 2 cut(s) 503, 2891
HspAI GCGC 1 cut(s) 2891
KasI GGCGCC 1 cut(s) 2890
KroI GCCGGC 2 cut(s) 721, 2339
KroNI GCCGGC 2 cut(s) 723, 2341
Ksp22I TGATCA 4 cut(s) 522, 913, 2398, 2926
KspAI GTTAAC 2 cut(s) 1237, 2782
LmnI GCTCC 8 cut(s) 626, 694, 698, 811, 1601, 1621, 3220, 3323
MaeI CTAG 7 cut(s) 417, 998, 1683, 2427, 2963, 3068, 3197
MaeII ACGT 4 cut(s) 317, 2066, 2227, 2843
MaeIII GTNAC 9 cut(s) 504, 646, 1114, 1134, 1414, 1694, 1945, 2900, 3301
MfeI CAATTG 2 cut(s) 2010, 2673
MflI RGATCY 6 cut(s) 213, 1040, 1450, 1997, 2293, 3254
MhlI GDGCHC 6 cut(s) 631, 703, 1598, 1634, 3276, 3328
Mly113I GGCGCC 1 cut(s) 2891
MlyI GAGTC 7 cut(s) 101, 352, 634, 1261, 1691, 2528, 2950
MmeI TCCRAC 1 cut(s) 3220
Mph1103I ATGCAT 2 cut(s) 2212, 2996
MroNI GCCGGC 2 cut(s) 721, 2339
MroXI GAANNNNTTC 4 cut(s) 215, 1469, 2505, 2994
MslI CAYNNNNRTG 5 cut(s) 185, 1049, 1637, 2909, 3149
MspA1I CMGCKG 2 cut(s) 740, 2882
MspCI CTTAAG 2 cut(s) 2002, 2786
MspI CCGG 4 cut(s) 174, 722, 809, 2340
MspR9I CCNGG 5 cut(s) 175, 991, 1600, 1620, 1706
MunI CAATTG 2 cut(s) 2010, 2673
Mva1269I GAATGC 5 cut(s) 757, 1382, 2343, 2994, 2996
MvaI CCWGG 4 cut(s) 991, 1600, 1620, 1706
NaeI GCCGGC 2 cut(s) 723, 2341
NarI GGCGCC 1 cut(s) 2891
NciI CCSGG 1 cut(s) 175
NcoI CCATGG 1 cut(s) 2073
NgoMIV GCCGGC 2 cut(s) 721, 2339
NheI GCTAGC 1 cut(s) 3196
NlaIV GGNNCC 9 cut(s) 1042, 1597, 2079, 2295, 2338, 2369, 2616, 2892, 3222
NmuCI GTSAC 3 cut(s) 504, 1694, 2900
NsiI ATGCAT 2 cut(s) 2212, 2996
NspI RCATGY 4 cut(s) 986, 1422, 2556, 3042
OliI CACNNNNGTG 2 cut(s) 185, 2909
PagI TCATGA 2 cut(s) 916, 1486
PciI ACATGT 2 cut(s) 1418, 3038
PctI GAATGC 5 cut(s) 757, 1382, 2343, 2994, 2996
PdiI GCCGGC 2 cut(s) 723, 2341
PdmI GAANNNNTTC 4 cut(s) 215, 1469, 2505, 2994
PfeI GAWTC 8 cut(s) 99, 196, 1220, 1917, 1933, 1965, 2149, 2687
PflMI CCANNNNNTGG 2 cut(s) 881, 1342
PleI GAGTC 7 cut(s) 100, 352, 634, 1260, 1691, 2528, 2950
PluTI GGCGCC 1 cut(s) 2894
PpsI GAGTC 7 cut(s) 100, 352, 634, 1260, 1691, 2528, 2950
Ppu21I YACGTR 1 cut(s) 2844
PpuMI RGGWCCY 1 cut(s) 1743
PscI ACATGT 2 cut(s) 1418, 3038
PshBI ATTAAT 1 cut(s) 2100
Psp124BI GAGCTC 1 cut(s) 631
Psp1406I AACGTT 1 cut(s) 2227
Psp5II RGGWCCY 1 cut(s) 1743
Psp6I CCWGG 4 cut(s) 989, 1598, 1618, 1704
PspGI CCWGG 4 cut(s) 989, 1598, 1618, 1704
PspN4I GGNNCC 9 cut(s) 1042, 1597, 2079, 2295, 2338, 2369, 2616, 2892, 3222
PspPI GGNCC 4 cut(s) 601, 710, 1743, 2368
PspPPI RGGWCCY 1 cut(s) 1743
PstI CTGCAG 2 cut(s) 739, 3219
PstNI CAGNNNCTG 3 cut(s) 2810, 3284, 3311
PsuI RGATCY 6 cut(s) 213, 1040, 1450, 1997, 2293, 3254
PvuII CAGCTG 2 cut(s) 740, 2882
RseI CAYNNNNRTG 5 cut(s) 185, 1049, 1637, 2909, 3149
SacI GAGCTC 1 cut(s) 631
Sau96I GGNCC 4 cut(s) 601, 710, 1743, 2368
ScaI AGTACT 1 cut(s) 119
SchI GAGTC 7 cut(s) 101, 352, 634, 1261, 1691, 2528, 2950
ScrFI CCNGG 5 cut(s) 175, 991, 1600, 1620, 1706
SduI GDGCHC 6 cut(s) 631, 703, 1598, 1634, 3276, 3328
SfcI CTRYAG 3 cut(s) 735, 1747, 3215
SfoI GGCGCC 1 cut(s) 2892
SinI GGWCC 4 cut(s) 601, 710, 1743, 2368
SmiMI CAYNNNNRTG 5 cut(s) 185, 1049, 1637, 2909, 3149
SmlI CTYRAG 5 cut(s) 47, 83, 1912, 2002, 2786
SmoI CTYRAG 5 cut(s) 47, 83, 1912, 2002, 2786
SpeI ACTAGT 2 cut(s) 1682, 3067
SsiI CCGC 8 cut(s) 414, 719, 940, 1205, 2559, 3011, 3077, 3194
SspDI GGCGCC 1 cut(s) 2890
SspI AATATT 2 cut(s) 1531, 3097
SspMI CTAG 7 cut(s) 417, 998, 1683, 2427, 2963, 3068, 3197
SstI GAGCTC 1 cut(s) 631
StyD4I CCNGG 5 cut(s) 173, 989, 1598, 1618, 1704
StyI CCWWGG 3 cut(s) 183, 566, 2073
TaaI ACNGT 9 cut(s) 1241, 1361, 1649, 2264, 2495, 3003, 3062, 3187, 3307
TaiI ACGT 4 cut(s) 320, 2069, 2230, 2846
TatI WGTACW 8 cut(s) 73, 117, 333, 1025, 1100, 1644, 3019, 3153
TauI GCSGC 2 cut(s) 1208, 3013
TfiI GAWTC 8 cut(s) 99, 196, 1220, 1917, 1933, 1965, 2149, 2687
TscAI CASTG 9 cut(s) 1366, 1631, 1654, 2267, 2401, 2428, 3008, 3156, 3312
TseFI GTSAC 3 cut(s) 504, 1694, 2900
Tsp45I GTSAC 3 cut(s) 504, 1694, 2900
TspGWI ACGGA 1 cut(s) 3336
TspRI CASTG 9 cut(s) 1366, 1631, 1654, 2267, 2401, 2428, 3008, 3156, 3312
Van91I CCANNNNNTGG 2 cut(s) 881, 1342
Vha464I CTTAAG 2 cut(s) 2002, 2786
VneI GTGCAC 1 cut(s) 1630
VpaK11BI GGWCC 4 cut(s) 601, 710, 1743, 2368
VspI ATTAAT 1 cut(s) 2100
XapI RAATTY 3 cut(s) 59, 1838, 2329
XceI RCATGY 4 cut(s) 986, 1422, 2556, 3042
XcmI CCANNNNNNNNNTGG 1 cut(s) 1167
XmnI GAANNNNTTC 4 cut(s) 215, 1469, 2505, 2994
XspI CTAG 7 cut(s) 417, 998, 1683, 2427, 2963, 3068, 3197
ZrmI AGTACT 1 cut(s) 119
Zsp2I ATGCAT 2 cut(s) 2212, 2996
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.