FvH4_4g18910

Topless-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0013792
Physical Location & Seq
Forward (+)
22688984 .. 22697149
8166 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g18910.t1

Sequence Viewer

Length: 2838 bp
ATGTCATCTCTGAGCAGAGAGCTCGTTTTCCTCATCCTCCAGTTCCTCGAGGAGGAGAAATTCAAGGAGGCCGTGCACAGGCTAGAGCAAGAGTCTGGATTCTTCTTCAACATGAAGTACTTCGAGGAGAAGGCTCTGCTGGGAGAGTGGGATGAGGTGGAGAAGTACCTGTCCGGGTTCACCAAGGTGGACGAGAATCGCTACTCCATGAAGATCTTCTTCGAGGTCAGAAAGCAGAAGTATCTCGAAGCGCTCGACAGGCAGGACAGGGCGAAGGCCGTGGAGATACTGGTGAAGGATTTGAAGGTGTTTTCGACGTTTAATGAGGAGTTGTACAAGGAAATCACGCATCTGTTGACTCTTGACAACTTCAGGGAAAATGAGCAGTTGTCGAAGTATGGTGATACTAAATCTGCGAGAAGCATAATGCTAGTAGAGCTGAAGAAATTGATAGAAGCGAATCCTCTGTTCCGAGAGAAGCTAGTTTTCCCCACTTTGAAGGCGTCACGCTTGCGAACTTTGATCAATCAAAGTTTAAATTGGCAGCACCAGCTATGTAAGAACCCAAGGCCAAATCCTGACATTAAGACCTTATTCATGGACCACTCGTGTTCTCCTCCAAATGGAGCTCGTGCATCAACCCCAGTTACTCTTCCAGTTGCAGCTCTTACAAAGCCTTTAACTTATGCTCCTCTTGGAGTACATGGTGGACCATTCCCTCCGGCTGCGGCTGCAGCAGCTGCCAATGCGAATGCTTTAGCAGGATGGATGTCCAATGCTAATCCTTCCTTGTCTATGCAATCAGCTCCAGTCGCTGCCTCACCCATGCCTGTTCAACTTGGTCAAGTTTCTTCATTAAAGCATCCAAGAACACCATCAACTGGTCTTGGAATGATTGATTATCAAAGCTCTGATCATGAGCAATTAATGAAACGCCTGCGGACTGCACAATCTGTTGACGAGGTTAGCTATCCTCCCCAACATGCTTCGTGGTCACTAGATGACCTACCGAGAAGTGTAGCTTGTTCACTTCGTCAGGGATCCACTGTTATGAGCATGGATTTCCACCCTTCACATCACACATTACTTGCAGTTGGCTGTAATAATGGAGAAGTTACTCTGTGGGAAGTTGGGTTGCGAGAGAGGCTGGTATCCAAGCCATTCAAAGTATGGGATATGGCTGCTTGTTCTGTGCCATTTCAGGGGGCTATTGCTAAGGATCCATCAATGTCTATTACCCGTGTATCATGGAGTCCAGATGGAAACTTAATTGGTGTTGCATTTAAGAAACATTTGGTTCATCTGTATGCTTATCATGGGTCTAATGATCTACGCCAACATTTGGAGATTGATGCCCACAGTGGTGGTGTGAATGACTTGGCATTCTCTCATCCAAACAAGCAATTATGTGTTGTAACATGTGGAGATGATAAGCTGATAAAGGTTTGGGATCTGAATGGAAGAAAGCTTTTCCACTTTGAAGGTCATGAAGCGCCTGTTTACTCGGTTTGCCCCCACCAGAAGGAGAATATTCAGTTTATATTTTCAACGGCTGTTGATGGGAAAATAAAAGCTTGGCTGTATGATAATGTGGGCTCCAGGGTTGACTATGACGCTCCTGGGCAGTATTGCACCACAATGCTGTACAGTGATGATGGAAATAGATTGTTCTCTTGTGGGACTAGTAAAGATGGTGACTCCTTCCTGGTTGAATGGAATGAAAGTGAAGGGGCAATAAAGAGGACGTATAGTGGGTTCAGAAAGAAATCTACTGGCATTGCTCAATTTGATACTACAAGAAATCACTTTTTGGCTGTTGGTGAAGATAGCCAAATAAAATTTTGGGATATGGATAACACAAATGTTCTTACAATTACAGATGCTGATGGTGGACTTCCATCTATTCCTCGCTTGAGATTCAATAAGGAAGGAAATCTGCTTGTTGTTACCACGGCTGACAATGGATTTAAGGTTCTTGCAAATGCTGATGGTCTGAGATCCTTAAGAGCGATTGAAAGTCGATCTTATGAAGCATCAAGAACACCTATTGAGATGAAGGTATCTACATCGGCCATGGTTCCAAATATCAATCCCGCCATTAATAAAGTAGACCGTATGGATGCAAGCTCTCCTGCCAGGCCTACTCAGATTCTTAATGGAGTTGATCCTTTGGCTAGGAGTGTAGAAAAGCGAAGACTATTAGATGATGCATCTGATAAAAGTAAACGTTGGGAACTGGCAGAGATTGTTGATCCTGTTCACTGTCGAGTGGCTACGATGCCAGATAGTAAAGATCCTGCTAACAAGGTTGCTCGACTTCTTTACACAAATTCGGGCGCCGGCATTCTGGCTCTTGGTTCAAATGGTGTCCAGAAGCTATGGAAATGGAGAAGTAATGATGAAAATCCAAGTGGAAAGGCCACCGCTAGTGTCATTCCGCAACATTGGCAACCCAACAGTGGTCTTTTGATGACTAATGATGTCCCTGAAAACTGTGAGGAAGCAGTTCCATGTATTGCTCTCTCAAAGAATGATTCCTATGTGATGTCTGCATGCGGCGGAAAAGTATCTCTGTTTAATATGATGACATTCAAGGTAATGACAACTTTCATGTCACCTCCACCAGCCTCAACCTTCCTGGCATTTCATCCTCGGGATAATAACATCATAGCAATTGGAACACAAGATTCAAGTATCCACATTTACAATGTTAGAGTTGATGAGGTCAAATCAAAACTGAAAGGCCACCAGAAGCACATTACCGGATTAGCCTTTTCTGTTGACCTAAAGATCATGGTTTCATCAGCTATGCTTTTGGAACATGGATACATGGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

946

Amino Acids

105.16

Weight (kDa)

7.36

Isoelectric Point (pI)

41.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH_TPL PF17814 5 - 34 2.9e-11 LisH-like dimerisation domain
TPR1-like_2nd PF21889 37 - 161 3.3e-61 TPR1-like, CTLH-containing domain
zf_topless PF21359 164 - 204 2.3e-25 TOPLESS, zinc finger domain
Beta-prop_EML_2 PF23414 330 - 467 1.4e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd PF25172 349 - 563 2.5e-09 WDR3 second beta-propeller domain
Beta-prop_RIG_2nd PF23775 358 - 499 2.3e-07 RIG second beta-propeller
Beta-prop_WDR3_1st PF25173 389 - 526 1e-12 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 406 - 491 1.1e-08 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 407 - 560 5.4e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 409 - 623 1.7e-22 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 411 - 663 6.8e-20 THOC3 beta-propeller domain
WDR55 PF24796 411 - 526 1.5e-07 WDR55
Beta-prop_EML PF23409 448 - 652 7.1e-09 Echinoderm microtubule-associated protein first beta-propeller
WD40 PF00400 449 - 484 2.9e-06 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 457 - 529 2.6e-08 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 464 - 571 1.1e-06 Echinoderm microtubule-associated protein second beta-propeller
EIF3I PF24805 471 - 668 7.9e-06 EIF3I
WDR55 PF24796 512 - 658 1.1e-06 WDR55
Beta-prop_WDR3_1st PF25173 519 - 658 5.9e-08 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 825 - 926 2.8e-11 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 830 - 936 3.3e-11 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 831 - 936 4.6e-06 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 831 - 935 4.1e-08 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 831 - 935 4e-09 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 833 - 937 7.5e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 833 - 936 4.5e-09 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 836 - 935 6.2e-08 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 839 - 935 3.8e-06 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 853 - 935 5.4e-08 WDR36/Utp21 first beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 855 - 936 9.3e-07 WDR90/POC16, second beta-propeller
Beta-prop_RIG_1st PF23770 863 - 925 4.6e-06 RIG first beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013792)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G16830
fragaria_vesca FvH4_4g18910 FvH4_4g18910
malus_domestica MD13G1162100.v1.1 MD16G1161900.v1.1
prunus_persica Prupe.1G129400_v2.0.a1
pyrus_communis pycom13g13830
rosa_chinensis RchiOBHm_Chr4g0423811
rosa_laevigata RLG00000007530
rosa_multiflora Rmu_sc0003369.1_g000018
rosa_roxburghii Rroxscaffold_5G00365920
rosa_rugosa Rorug04G0190900
rosa_samantha Rh4AG250300 Rh4BG255600 Rh4CG263700 Rh4DG250000
rosa_wichuraiana Rw4G021430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 47
AccB1I GGYRCC 1 cut(s) 2336
AccB7I CCANNNNNTGG 2 cut(s) 881, 1342
AccI GTMKAC 1 cut(s) 2109
AciI CCGC 7 cut(s) 728, 940, 2094, 2424, 2438, 2556, 2559
AclI AACGTT 1 cut(s) 2227
AclWI GGATC 9 cut(s) 1035, 1048, 1214, 1227, 1458, 1992, 2159, 2246, 2288
AcoI YGGCCR 1 cut(s) 2070
AcsI RAATTY 3 cut(s) 59, 1838, 2329
AcuI CTGAAG 2 cut(s) 355, 461
AcyI GRCGYC 2 cut(s) 503, 2337
AfaI GTAC 5 cut(s) 119, 167, 335, 702, 1646
AfeI AGCGCT 1 cut(s) 252
AfiI CCNNNNNNNGG 8 cut(s) 52, 78, 623, 881, 1202, 1342, 1522, 2459
AflII CTTAAG 1 cut(s) 2002
AflIII ACRYGT 1 cut(s) 1418
AhlI ACTAGT 1 cut(s) 1682
AjnI CCWGG 5 cut(s) 1598, 1618, 1704, 2135, 2637
AleI CACNNNNGTG 2 cut(s) 185, 1362
AloI GAACNNNNNNTCC 2 cut(s) 1956, 1988
Alw21I GWGCWC 3 cut(s) 24, 78, 631
Alw44I GTGCAC 1 cut(s) 74
AlwI GGATC 9 cut(s) 1035, 1048, 1214, 1227, 1458, 1992, 2159, 2246, 2288
AlwNI CAGNNNCTG 3 cut(s) 740, 815, 1883
Ama87I CYCGRG 2 cut(s) 47, 2652
Aor51HI AGCGCT 1 cut(s) 252
AoxI GGCC 7 cut(s) 69, 276, 569, 2070, 2138, 2418, 2743
ApaLI GTGCAC 1 cut(s) 74
ApeKI GCWGC 9 cut(s) 544, 662, 725, 731, 734, 737, 740, 815, 1181
ApoI RAATTY 3 cut(s) 59, 1838, 2329
AseI ATTAAT 2 cut(s) 926, 2100
Asp700I GAANNNNTTC 3 cut(s) 119, 215, 2505
AspLEI GCGC 3 cut(s) 253, 1495, 2339
AspS9I GGNCC 2 cut(s) 601, 710
AsuC2I CCSGG 1 cut(s) 175
AsuHPI GGTGA 7 cut(s) 172, 304, 413, 813, 1706, 1832, 2607
AvaI CYCGRG 2 cut(s) 47, 2652
AvaII GGWCC 2 cut(s) 601, 710
BaeGI GKGCMC 1 cut(s) 78
BaeI ACNNNNGTAYC 2 cut(s) 1227, 1260
BamHI GGATCC 2 cut(s) 1040, 1219
BanI GGYRCC 1 cut(s) 2336
BanII GRGCYC 3 cut(s) 24, 631, 1598
BauI CACGAG 2 cut(s) 607, 630
BbsI GAAGAC 1 cut(s) 2200
Bbv12I GWGCWC 3 cut(s) 24, 78, 631
BbvI GCAGC 9 cut(s) 556, 674, 712, 718, 727, 746, 749, 802, 1168
BceAI ACGGC 4 cut(s) 56, 263, 1566, 1968
BcgI CGANNNNNNTGC 2 cut(s) 4, 38
BciT130I CCWGG 5 cut(s) 1600, 1620, 1706, 2137, 2639
BciVI GTATCC 3 cut(s) 1162, 2705, 2819
BclI TGATCA 2 cut(s) 522, 913
BcnI CCSGG 1 cut(s) 175
BcuI ACTAGT 1 cut(s) 1682
BfaI CTAG 7 cut(s) 83, 431, 482, 998, 1683, 2175, 2427
BfmI CTRYAG 1 cut(s) 732
BfoI RGCGCY 3 cut(s) 254, 1496, 2340
BfrI CTTAAG 1 cut(s) 2002
BfuI GTATCC 3 cut(s) 1162, 2705, 2819
BglII AGATCT 1 cut(s) 213
BmcAI AGTACT 1 cut(s) 119
Bme1390I CCNGG 6 cut(s) 175, 1600, 1620, 1706, 2137, 2639
Bme18I GGWCC 2 cut(s) 601, 710
BmeT110I CYCGRG 2 cut(s) 47, 2652
BmgT120I GGNCC 2 cut(s) 601, 710
BmiI GGNNCC 5 cut(s) 1042, 1221, 1597, 2079, 2338
BmrFI CCNGG 6 cut(s) 175, 1600, 1620, 1706, 2137, 2639
BmrI ACTGGG 1 cut(s) 638
BmuI ACTGGG 1 cut(s) 638
BpiI GAAGAC 1 cut(s) 2200
BpmI CTGGAG 3 cut(s) 23, 792, 1582
Bpu10I CCTNAGC 1 cut(s) 1215
BpuEI CTTGAG 1 cut(s) 1933
BpuMI CCSGG 1 cut(s) 175
BsaBI GATNNNNATC 1 cut(s) 2403
BsaHI GRCGYC 2 cut(s) 503, 2337
BsaJI CCNNGG 8 cut(s) 183, 279, 566, 1599, 1619, 1950, 2073, 2651
BsaWI WCCGGW 1 cut(s) 2762
BsaXI ACNNNNNCTCC 2 cut(s) 673, 703
Bsc4I CCNNNNNNNGG 8 cut(s) 52, 78, 623, 881, 1202, 1342, 1522, 2459
Bse118I RCCGGY 1 cut(s) 2339
Bse1I ACTGG 8 cut(s) 40, 294, 644, 656, 809, 886, 1777, 2241
Bse3DI GCAATG 1 cut(s) 1776
Bse8I GATNNNNATC 1 cut(s) 2403
BseBI CCWGG 5 cut(s) 1600, 1620, 1706, 2137, 2639
BseDI CCNNGG 8 cut(s) 183, 279, 566, 1599, 1619, 1950, 2073, 2651
BseGI GGATG 8 cut(s) 33, 157, 770, 774, 862, 1390, 2125, 2647
BseJI GATNNNNATC 1 cut(s) 2403
BseLI CCNNNNNNNGG 8 cut(s) 52, 78, 623, 881, 1202, 1342, 1522, 2459
BseMI GCAATG 1 cut(s) 1776
BseMII CTCAG 2 cut(s) 1985, 2159
BseNI ACTGG 8 cut(s) 40, 294, 644, 656, 809, 886, 1777, 2241
BseRI GAGGAG 6 cut(s) 65, 68, 140, 341, 606, 681
BseSI GKGCMC 1 cut(s) 78
BseXI GCAGC 9 cut(s) 556, 674, 712, 718, 727, 746, 749, 802, 1168
BseYI CCCAGC 1 cut(s) 139
BsgI GTGCAG 1 cut(s) 930
BshFI GGCC 7 cut(s) 71, 278, 571, 2072, 2140, 2420, 2745
BshNI GGYRCC 1 cut(s) 2336
BsiHKAI GWGCWC 3 cut(s) 24, 78, 631
BsiHKCI CYCGRG 2 cut(s) 47, 2652
BsiSI CCGG 4 cut(s) 174, 722, 2340, 2763
BslFI GGGAC 2 cut(s) 1693, 2468
BslI CCNNNNNNNGG 8 cut(s) 52, 78, 623, 881, 1202, 1342, 1522, 2459
BsmFI GGGAC 2 cut(s) 1693, 2468
BsmI GAATGC 3 cut(s) 757, 1382, 2343
BsnI GGCC 7 cut(s) 71, 278, 571, 2072, 2140, 2420, 2745
BsoBI CYCGRG 2 cut(s) 47, 2652
Bsp1286I GDGCHC 4 cut(s) 24, 78, 631, 1598
Bsp1407I TGTACA 2 cut(s) 333, 1644
Bsp19I CCATGG 1 cut(s) 2073
BspACI CCGC 7 cut(s) 728, 940, 2094, 2424, 2438, 2556, 2559
BspANI GGCC 7 cut(s) 71, 278, 571, 2072, 2140, 2420, 2745
BspCNI CTCAG 2 cut(s) 1986, 2158
BspHI TCATGA 2 cut(s) 916, 1486
BspLI GGNNCC 5 cut(s) 1042, 1221, 1597, 2079, 2338
BspMAI CTGCAG 1 cut(s) 736
BspPI GGATC 9 cut(s) 1035, 1048, 1214, 1227, 1458, 1992, 2159, 2246, 2288
BspT107I GGYRCC 1 cut(s) 2336
BspTI CTTAAG 1 cut(s) 2002
BsrDI GCAATG 1 cut(s) 1776
BsrFI RCCGGY 1 cut(s) 2339
BsrGI TGTACA 2 cut(s) 333, 1644
BsrI ACTGG 8 cut(s) 40, 294, 644, 656, 809, 886, 1777, 2241
BssAI RCCGGY 1 cut(s) 2339
BssECI CCNNGG 8 cut(s) 183, 279, 566, 1599, 1619, 1950, 2073, 2651
BssNI GRCGYC 2 cut(s) 503, 2337
BssSI CACGAG 2 cut(s) 607, 630
BssT1I CCWWGG 3 cut(s) 183, 566, 2073
Bst2BI CACGAG 2 cut(s) 607, 630
Bst2UI CCWGG 5 cut(s) 1600, 1620, 1706, 2137, 2639
Bst4CI ACNGT 7 cut(s) 1048, 1361, 1649, 2114, 2264, 2459, 2495
Bst6I CTCTTC 1 cut(s) 657
BstACI GRCGYC 2 cut(s) 503, 2337
BstAFI CTTAAG 1 cut(s) 2002
BstAPI GCANNNNNTGC 1 cut(s) 740
BstAUI TGTACA 2 cut(s) 333, 1644
BstC8I GCNNGC 5 cut(s) 512, 938, 2125, 2341, 2554
BstDEI CTNAG 4 cut(s) 11, 1215, 1994, 2145
BstDSI CCRYGG 3 cut(s) 279, 1950, 2073
BstENI CCTNNNNNAGG 1 cut(s) 50
BstF5I GGATG 8 cut(s) 33, 157, 770, 774, 862, 1390, 2125, 2647
BstH2I RGCGCY 3 cut(s) 254, 1496, 2340
BstHHI GCGC 3 cut(s) 253, 1495, 2339
BstNI CCWGG 5 cut(s) 1600, 1620, 1706, 2137, 2639
BstNSI RCATGY 3 cut(s) 986, 1422, 2556
BstSCI CCNGG 6 cut(s) 173, 1598, 1618, 1704, 2135, 2637
BstSFI CTRYAG 1 cut(s) 732
BstSLI GKGCMC 1 cut(s) 78
BstV1I GCAGC 9 cut(s) 556, 674, 712, 718, 727, 746, 749, 802, 1168
BstV2I GAAGAC 1 cut(s) 2200
BstX2I RGATCY 6 cut(s) 213, 1040, 1219, 1450, 1997, 2293
BstXI CCANNNNNNTGG 1 cut(s) 1364
BstYI RGATCY 6 cut(s) 213, 1040, 1219, 1450, 1997, 2293
BsuI GTATCC 3 cut(s) 1162, 2705, 2819
BsuRI GGCC 7 cut(s) 71, 278, 571, 2072, 2140, 2420, 2745
BtgI CCRYGG 3 cut(s) 279, 1950, 2073
BtsCI GGATG 8 cut(s) 33, 157, 770, 774, 862, 1390, 2125, 2647
BtsIMutI CAGTG 5 cut(s) 1044, 1366, 1654, 2260, 2464
Cac8I GCNNGC 5 cut(s) 512, 938, 2125, 2341, 2554
CaiI CAGNNNCTG 3 cut(s) 740, 815, 1883
CciI TCATGA 2 cut(s) 916, 1486
CfoI GCGC 3 cut(s) 253, 1495, 2339
Cfr10I RCCGGY 1 cut(s) 2339
Cfr13I GGNCC 2 cut(s) 601, 710
CseI GACGC 2 cut(s) 492, 1622
Csp6I GTAC 5 cut(s) 118, 166, 334, 701, 1645
CviQI GTAC 5 cut(s) 118, 166, 334, 701, 1645
DdeI CTNAG 4 cut(s) 11, 1215, 1994, 2145
DinI GGCGCC 1 cut(s) 2338
DraI TTTAAA 1 cut(s) 537
EaeI YGGCCR 1 cut(s) 2070
Eam1104I CTCTTC 1 cut(s) 657
EarI CTCTTC 1 cut(s) 657
EciI GGCGGA 1 cut(s) 2574
Ecl136II GAGCTC 2 cut(s) 22, 629
Eco130I CCWWGG 3 cut(s) 183, 566, 2073
Eco147I AGGCCT 1 cut(s) 2140
Eco24I GRGCYC 3 cut(s) 24, 631, 1598
Eco47I GGWCC 2 cut(s) 601, 710
Eco47III AGCGCT 1 cut(s) 252
Eco53kI GAGCTC 2 cut(s) 22, 629
Eco57I CTGAAG 2 cut(s) 355, 461
Eco88I CYCGRG 2 cut(s) 47, 2652
EcoICRI GAGCTC 2 cut(s) 22, 629
EcoNI CCTNNNNNAGG 1 cut(s) 50
EcoRII CCWGG 5 cut(s) 1598, 1618, 1704, 2135, 2637
EcoT14I CCWWGG 3 cut(s) 183, 566, 2073
EcoT22I ATGCAT 1 cut(s) 2212
EcoT38I GRGCYC 3 cut(s) 24, 631, 1598
EgeI GGCGCC 1 cut(s) 2338
EheI GGCGCC 1 cut(s) 2338
ErhI CCWWGG 3 cut(s) 183, 566, 2073
FalI AAGNNNNNCTT 6 cut(s) 203, 235, 1006, 1038, 2008, 2040
FaqI GGGAC 2 cut(s) 1693, 2468
FauI CCCGC 1 cut(s) 2101
FbaI TGATCA 2 cut(s) 522, 913
FblI GTMKAC 1 cut(s) 2109
FokI GGATG 8 cut(s) 20, 164, 777, 781, 849, 1377, 2132, 2634
FriOI GRGCYC 3 cut(s) 24, 631, 1598
FspBI CTAG 7 cut(s) 83, 431, 482, 998, 1683, 2175, 2427
GlaI GCGC 3 cut(s) 252, 1494, 2338
GsaI CCCAGC 1 cut(s) 143
GsuI CTGGAG 3 cut(s) 23, 792, 1582
HaeII RGCGCY 3 cut(s) 254, 1496, 2340
HaeIII GGCC 7 cut(s) 71, 278, 571, 2072, 2140, 2420, 2745
HapII CCGG 4 cut(s) 174, 722, 2340, 2763
HgaI GACGC 2 cut(s) 492, 1622
HhaI GCGC 3 cut(s) 253, 1495, 2339
Hin1I GRCGYC 2 cut(s) 503, 2337
Hin6I GCGC 3 cut(s) 251, 1493, 2337
HinP1I GCGC 3 cut(s) 251, 1493, 2337
HincII GTYRAC 4 cut(s) 357, 958, 1606, 2782
HindII GTYRAC 4 cut(s) 357, 958, 1606, 2782
HindIII AAGCTT 2 cut(s) 1466, 1572
HpaII CCGG 4 cut(s) 174, 722, 2340, 2763
HphI GGTGA 7 cut(s) 172, 304, 413, 813, 1706, 1832, 2607
Hpy188I TCNGA 9 cut(s) 12, 230, 473, 913, 1455, 1760, 1995, 2148, 2215
Hpy99I CGWCG 1 cut(s) 319
HpyCH4III ACNGT 7 cut(s) 1048, 1361, 1649, 2114, 2264, 2459, 2495
HpyCH4IV ACGT 3 cut(s) 317, 1745, 2227
HpyF3I CTNAG 4 cut(s) 11, 1215, 1994, 2145
HpySE526I ACGT 3 cut(s) 317, 1745, 2227
Hsp92I GRCGYC 2 cut(s) 503, 2337
HspAI GCGC 3 cut(s) 251, 1493, 2337
KasI GGCGCC 1 cut(s) 2336
KroI GCCGGC 1 cut(s) 2339
KroNI GCCGGC 1 cut(s) 2341
Ksp22I TGATCA 2 cut(s) 522, 913
LmnI GCTCC 5 cut(s) 626, 694, 811, 1601, 1621
Lsp1109I GCAGC 9 cut(s) 556, 674, 712, 718, 727, 746, 749, 802, 1168
MaeI CTAG 7 cut(s) 83, 431, 482, 998, 1683, 2175, 2427
MaeII ACGT 3 cut(s) 317, 1745, 2227
MaeIII GTNAC 8 cut(s) 504, 646, 993, 1114, 1414, 1694, 1945, 2613
MfeI CAATTG 1 cut(s) 2673
MflI RGATCY 6 cut(s) 213, 1040, 1219, 1450, 1997, 2293
MhlI GDGCHC 4 cut(s) 24, 78, 631, 1598
Mly113I GGCGCC 1 cut(s) 2337
MlyI GAGTC 4 cut(s) 101, 352, 1261, 1691
Mph1103I ATGCAT 1 cut(s) 2212
MroNI GCCGGC 1 cut(s) 2339
MroXI GAANNNNTTC 3 cut(s) 119, 215, 2505
MslI CAYNNNNRTG 5 cut(s) 185, 1049, 1305, 1362, 1637
MspA1I CMGCKG 1 cut(s) 740
MspCI CTTAAG 1 cut(s) 2002
MspI CCGG 4 cut(s) 174, 722, 2340, 2763
MspR9I CCNGG 6 cut(s) 175, 1600, 1620, 1706, 2137, 2639
MunI CAATTG 1 cut(s) 2673
Mva1269I GAATGC 3 cut(s) 757, 1382, 2343
MvaI CCWGG 5 cut(s) 1600, 1620, 1706, 2137, 2639
NaeI GCCGGC 1 cut(s) 2341
NarI GGCGCC 1 cut(s) 2337
NciI CCSGG 1 cut(s) 175
NcoI CCATGG 1 cut(s) 2073
NgoMIV GCCGGC 1 cut(s) 2339
NlaIV GGNNCC 5 cut(s) 1042, 1221, 1597, 2079, 2338
NmuCI GTSAC 4 cut(s) 504, 993, 1694, 2613
NsiI ATGCAT 1 cut(s) 2212
NspI RCATGY 3 cut(s) 986, 1422, 2556
OliI CACNNNNGTG 2 cut(s) 185, 1362
PaeI GCATGC 1 cut(s) 2556
PaeR7I CTCGAG 1 cut(s) 47
PagI TCATGA 2 cut(s) 916, 1486
PceI AGGCCT 1 cut(s) 2140
PciI ACATGT 1 cut(s) 1418
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PctI GAATGC 3 cut(s) 757, 1382, 2343
PdiI GCCGGC 1 cut(s) 2341
PdmI GAANNNNTTC 3 cut(s) 119, 215, 2505
PfeI GAWTC 7 cut(s) 99, 196, 460, 1917, 2149, 2534, 2687
PflMI CCANNNNNTGG 2 cut(s) 881, 1342
PleI GAGTC 4 cut(s) 100, 352, 1260, 1691
PluTI GGCGCC 1 cut(s) 2340
PpsI GAGTC 4 cut(s) 100, 352, 1260, 1691
PscI ACATGT 1 cut(s) 1418
PshBI ATTAAT 2 cut(s) 926, 2100
Psp124BI GAGCTC 2 cut(s) 24, 631
Psp1406I AACGTT 1 cut(s) 2227
Psp6I CCWGG 5 cut(s) 1598, 1618, 1704, 2135, 2637
PspFI CCCAGC 1 cut(s) 139
PspGI CCWGG 5 cut(s) 1598, 1618, 1704, 2135, 2637
PspN4I GGNNCC 5 cut(s) 1042, 1221, 1597, 2079, 2338
PspPI GGNCC 2 cut(s) 601, 710
PspXI VCTCGAGB 1 cut(s) 47
PsrI GAACNNNNNNTAC 2 cut(s) 1739, 1771
PstI CTGCAG 1 cut(s) 736
PstNI CAGNNNCTG 3 cut(s) 740, 815, 1883
PsuI RGATCY 6 cut(s) 213, 1040, 1219, 1450, 1997, 2293
PvuII CAGCTG 1 cut(s) 740
RsaI GTAC 5 cut(s) 119, 167, 335, 702, 1646
RsaNI GTAC 5 cut(s) 118, 166, 334, 701, 1645
RseI CAYNNNNRTG 5 cut(s) 185, 1049, 1305, 1362, 1637
SacI GAGCTC 2 cut(s) 24, 631
Sau96I GGNCC 2 cut(s) 601, 710
ScaI AGTACT 1 cut(s) 119
SchI GAGTC 4 cut(s) 101, 352, 1261, 1691
ScrFI CCNGG 6 cut(s) 175, 1600, 1620, 1706, 2137, 2639
SduI GDGCHC 4 cut(s) 24, 78, 631, 1598
SfcI CTRYAG 1 cut(s) 732
SfoI GGCGCC 1 cut(s) 2338
Sfr274I CTCGAG 1 cut(s) 47
SinI GGWCC 2 cut(s) 601, 710
SlaI CTCGAG 1 cut(s) 47
SmiMI CAYNNNNRTG 5 cut(s) 185, 1049, 1305, 1362, 1637
SmlI CTYRAG 3 cut(s) 47, 1912, 2002
SmoI CTYRAG 3 cut(s) 47, 1912, 2002
SpeI ACTAGT 1 cut(s) 1682
SphI GCATGC 1 cut(s) 2556
SseBI AGGCCT 1 cut(s) 2140
SsiI CCGC 7 cut(s) 728, 940, 2094, 2424, 2438, 2556, 2559
SspDI GGCGCC 1 cut(s) 2336
SspI AATATT 1 cut(s) 1531
SspMI CTAG 7 cut(s) 83, 431, 482, 998, 1683, 2175, 2427
SstI GAGCTC 2 cut(s) 24, 631
StuI AGGCCT 1 cut(s) 2140
StyD4I CCNGG 6 cut(s) 173, 1598, 1618, 1704, 2135, 2637
StyI CCWWGG 3 cut(s) 183, 566, 2073
TaaI ACNGT 7 cut(s) 1048, 1361, 1649, 2114, 2264, 2459, 2495
TaiI ACGT 3 cut(s) 320, 1748, 2230
TatI WGTACW 4 cut(s) 117, 333, 700, 1644
TauI GCSGC 2 cut(s) 731, 2559
TfiI GAWTC 7 cut(s) 99, 196, 460, 1917, 2149, 2534, 2687
TscAI CASTG 5 cut(s) 1051, 1366, 1654, 2267, 2464
TseFI GTSAC 4 cut(s) 504, 993, 1694, 2613
TseI GCWGC 9 cut(s) 544, 662, 725, 731, 734, 737, 740, 815, 1181
Tsp45I GTSAC 4 cut(s) 504, 993, 1694, 2613
TspRI CASTG 5 cut(s) 1051, 1366, 1654, 2267, 2464
Van91I CCANNNNNTGG 2 cut(s) 881, 1342
Vha464I CTTAAG 1 cut(s) 2002
VneI GTGCAC 1 cut(s) 74
VpaK11BI GGWCC 2 cut(s) 601, 710
VspI ATTAAT 2 cut(s) 926, 2100
XagI CCTNNNNNAGG 1 cut(s) 50
XapI RAATTY 3 cut(s) 59, 1838, 2329
XceI RCATGY 3 cut(s) 986, 1422, 2556
XcmI CCANNNNNNNNNTGG 1 cut(s) 1167
XhoI CTCGAG 1 cut(s) 47
XmiI GTMKAC 1 cut(s) 2109
XmnI GAANNNNTTC 3 cut(s) 119, 215, 2505
XspI CTAG 7 cut(s) 83, 431, 482, 998, 1683, 2175, 2427
ZrmI AGTACT 1 cut(s) 119
Zsp2I ATGCAT 1 cut(s) 2212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.