Rorug04G0190900

Topless-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0013792
Physical Location & Seq
Forward (+)
33118880 .. 33120945
2066 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0190900.1

Sequence Viewer

Length: 609 bp
ATGGACATTAACATTCTTGAGGATGGTGTAATGAGACTGGACATTTCAAATTGTACAGATGAACAAAATACGGCAGGAGCTGCAGTCATATTTGTCAAATGGACCAATTACAATTGTGACTGGGAGTCAAACAGTGCAGGTGCTAGCAGATATGGTATAATTATTGTTGCAGTAGTGGCAGGATATGGCTACTTTTGGTGGAAGGGGTGGAAACTTCCTGATATGATGTTTGCAACAAGACGTAGCTTAGCTGATGCTGGCAAGTCTTTTTCTAAACAGATTGACGGTGTCTTTTCATCGATTTCAATCACCAAGCGACAATTATCTTCAAACTTGGATGGTGTGGACTGTAATTTAGCTGAGAGTATTGAAACTACTGTTAGGACACAACAGGAGGTTATTGAACTACAAGGAAAAACAGATTCCATCAGCACGGATTTTAGAAAAGTTCAACATGCAGTGCTGACTCTGGAAACTAAGATTAACATAATAGAAGGCAAGCAGGATTCAACTAATGACGGAGTGTGGAGGTTGTGTGACTATGCCAAGAGATTGGAAAATAGCAGAACCACAGAACGTATTCAGGCATGTATTGAACTCTTCCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

202

Amino Acids

22.71

Weight (kDa)

5.01

Isoelectric Point (pI)

46.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1664 PF07889 49 - 168 1.3e-39 Protein of unknown function (DUF1664)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013792)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G16830
fragaria_vesca FvH4_4g18910 FvH4_4g18910
malus_domestica MD13G1162100.v1.1 MD16G1161900.v1.1
prunus_persica Prupe.1G129400_v2.0.a1
pyrus_communis pycom13g13830
rosa_chinensis RchiOBHm_Chr4g0423811
rosa_laevigata RLG00000007530
rosa_multiflora Rmu_sc0003369.1_g000018
rosa_roxburghii Rroxscaffold_5G00365920
rosa_rugosa Rorug04G0190900
rosa_samantha Rh4AG250300 Rh4BG255600 Rh4CG263700 Rh4DG250000
rosa_wichuraiana Rw4G021430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 128
Acc36I ACCTGC 1 cut(s) 128
AfaI GTAC 1 cut(s) 55
AgsI TTSAA 8 cut(s) 48, 306, 330, 371, 404, 452, 510, 596
AhdI GACNNNNNGTC 1 cut(s) 124
AluBI AGCT 4 cut(s) 80, 246, 251, 359
AluI AGCT 4 cut(s) 80, 246, 251, 359
Alw26I GTCTC 1 cut(s) 28
AlwNI CAGNNNCTG 1 cut(s) 80
ApeKI GCWGC 1 cut(s) 80
Asp700I GAANNNNTTC 1 cut(s) 579
AspS9I GGNCC 1 cut(s) 102
AsuHPI GGTGA 1 cut(s) 301
AsuNHI GCTAGC 1 cut(s) 143
AvaII GGWCC 1 cut(s) 102
BbvI GCAGC 1 cut(s) 67
BccI CCATC 3 cut(s) 17, 332, 434
BceAI ACGGC 1 cut(s) 87
BcoDI GTCTC 1 cut(s) 28
BfaI CTAG 1 cut(s) 144
BfmI CTRYAG 1 cut(s) 81
BfuAI ACCTGC 1 cut(s) 128
BisI GCNGC 1 cut(s) 81
BlpI GCTNAGC 1 cut(s) 247
BlsI GCNGC 1 cut(s) 82
Bme18I GGWCC 1 cut(s) 102
BmeRI GACNNNNNGTC 1 cut(s) 124
BmgT120I GGNCC 1 cut(s) 102
BmrI ACTGGG 1 cut(s) 130
BmsI GCATC 1 cut(s) 244
BmtI GCTAGC 1 cut(s) 147
BmuI ACTGGG 1 cut(s) 130
Bpu1102I GCTNAGC 1 cut(s) 247
BpuEI CTTGAG 1 cut(s) 38
Bsa29I ATCGAT 1 cut(s) 299
BsaBI GATNNNNATC 1 cut(s) 305
BsaXI ACNNNNNCTCC 4 cut(s) 69, 99, 520, 550
Bse1I ACTGG 2 cut(s) 42, 125
Bse8I GATNNNNATC 1 cut(s) 305
BseCI ATCGAT 1 cut(s) 299
BseGI GGATG 2 cut(s) 28, 343
BseJI GATNNNNATC 1 cut(s) 305
BseMII CTCAG 1 cut(s) 351
BseNI ACTGG 2 cut(s) 42, 125
BseXI GCAGC 1 cut(s) 67
BsgI GTGCAG 1 cut(s) 156
BshVI ATCGAT 1 cut(s) 299
BsmAI GTCTC 1 cut(s) 28
Bsp1407I TGTACA 1 cut(s) 53
Bsp1720I GCTNAGC 1 cut(s) 247
BspCNI CTCAG 1 cut(s) 352
BspDI ATCGAT 1 cut(s) 299
BspMAI CTGCAG 1 cut(s) 85
BspMI ACCTGC 1 cut(s) 128
BspOI GCTAGC 1 cut(s) 147
BsrGI TGTACA 1 cut(s) 53
BsrI ACTGG 2 cut(s) 42, 125
Bst4CI ACNGT 4 cut(s) 134, 287, 350, 379
Bst6I CTCTTC 1 cut(s) 605
BstAPI GCANNNNNTGC 1 cut(s) 80
BstAUI TGTACA 1 cut(s) 53
BstC8I GCNNGC 3 cut(s) 145, 259, 500
BstDEI CTNAG 3 cut(s) 247, 360, 477
BstF5I GGATG 2 cut(s) 28, 343
BstMAI GTCTC 1 cut(s) 28
BstMWI GCNNNNNNNGC 2 cut(s) 80, 176
BstNSI RCATGY 2 cut(s) 458, 591
BstSFI CTRYAG 1 cut(s) 81
BstV1I GCAGC 1 cut(s) 67
BstXI CCANNNNNNTGG 1 cut(s) 553
Bsu15I ATCGAT 1 cut(s) 299
BsuTUI ATCGAT 1 cut(s) 299
BtsCI GGATG 2 cut(s) 28, 343
BtsI GCAGTG 1 cut(s) 465
BtsIMutI CAGTG 2 cut(s) 139, 465
BveI ACCTGC 1 cut(s) 128
Cac8I GCNNGC 3 cut(s) 145, 259, 500
CaiI CAGNNNCTG 1 cut(s) 80
Cfr13I GGNCC 1 cut(s) 102
ClaI ATCGAT 1 cut(s) 299
Csp6I GTAC 1 cut(s) 54
CviAII CATG 2 cut(s) 455, 588
CviJI RGCY 5 cut(s) 80, 189, 246, 251, 359
CviKI_1 RGCY 5 cut(s) 80, 189, 246, 251, 359
CviQI GTAC 1 cut(s) 54
DdeI CTNAG 3 cut(s) 247, 360, 477
DriI GACNNNNNGTC 1 cut(s) 124
Eam1104I CTCTTC 1 cut(s) 605
Eam1105I GACNNNNNGTC 1 cut(s) 124
EarI CTCTTC 1 cut(s) 605
Eco47I GGWCC 1 cut(s) 102
FaeI CATG 2 cut(s) 458, 591
FaiI YATR 9 cut(s) 89, 153, 158, 186, 224, 456, 488, 543, 589
FatI CATG 2 cut(s) 454, 587
Fnu4HI GCNGC 1 cut(s) 81
FokI GGATG 2 cut(s) 35, 350
Fsp4HI GCNGC 1 cut(s) 81
FspBI CTAG 1 cut(s) 144
GluI GCNGC 1 cut(s) 81
Hin1II CATG 2 cut(s) 458, 591
HinfI GANTC 4 cut(s) 125, 422, 466, 506
HphI GGTGA 1 cut(s) 301
Hpy166II GTNNAC 1 cut(s) 346
Hpy188III TCNNGA 3 cut(s) 17, 218, 470
Hpy8I GTNNAC 1 cut(s) 346
HpyAV CCTTC 2 cut(s) 196, 488
HpyCH4III ACNGT 4 cut(s) 134, 287, 350, 379
HpyCH4IV ACGT 2 cut(s) 241, 577
HpyCH4V TGCA 5 cut(s) 83, 137, 170, 233, 458
HpyF10VI GCNNNNNNNGC 2 cut(s) 80, 176
HpyF3I CTNAG 3 cut(s) 247, 360, 477
HpySE526I ACGT 2 cut(s) 241, 577
Hsp92II CATG 2 cut(s) 458, 591
LmnI GCTCC 1 cut(s) 77
Lsp1109I GCAGC 1 cut(s) 67
LweI GCATC 1 cut(s) 244
MaeI CTAG 1 cut(s) 144
MaeII ACGT 2 cut(s) 241, 577
MaeIII GTNAC 2 cut(s) 116, 536
MboII GAAGA 2 cut(s) 318, 592
MfeI CAATTG 1 cut(s) 112
MluCI AATT 6 cut(s) 49, 106, 112, 159, 320, 352
MlyI GAGTC 2 cut(s) 134, 460
MnlI CCTC 3 cut(s) 13, 388, 522
MroXI GAANNNNTTC 1 cut(s) 579
MseI TTAA 2 cut(s) 9, 483
MunI CAATTG 1 cut(s) 112
MwoI GCNNNNNNNGC 2 cut(s) 80, 176
NheI GCTAGC 1 cut(s) 143
NlaIII CATG 2 cut(s) 458, 591
NmuCI GTSAC 2 cut(s) 116, 536
NspI RCATGY 2 cut(s) 458, 591
PaqCI CACCTGC 1 cut(s) 128
PdmI GAANNNNTTC 1 cut(s) 579
PfeI GAWTC 2 cut(s) 422, 506
PflFI GACNNNGTC 1 cut(s) 287
PkrI GCNGC 1 cut(s) 82
PleI GAGTC 2 cut(s) 133, 460
PpsI GAGTC 2 cut(s) 133, 460
PspPI GGNCC 1 cut(s) 102
PstI CTGCAG 1 cut(s) 85
PstNI CAGNNNCTG 1 cut(s) 80
PsyI GACNNNGTC 1 cut(s) 287
RsaI GTAC 1 cut(s) 55
RsaNI GTAC 1 cut(s) 54
SaqAI TTAA 2 cut(s) 9, 483
SatI GCNGC 1 cut(s) 81
Sau96I GGNCC 1 cut(s) 102
SchI GAGTC 2 cut(s) 134, 460
SetI ASST 9 cut(s) 82, 142, 244, 248, 253, 361, 399, 533, 580
SfaNI GCATC 1 cut(s) 244
SfcI CTRYAG 1 cut(s) 81
SinI GGWCC 1 cut(s) 102
SmlI CTYRAG 1 cut(s) 17
SmoI CTYRAG 1 cut(s) 17
Sse9I AATT 6 cut(s) 49, 106, 112, 159, 320, 352
SspMI CTAG 1 cut(s) 144
TaaI ACNGT 4 cut(s) 134, 287, 350, 379
TaiI ACGT 2 cut(s) 244, 580
TaqI TCGA 1 cut(s) 299
TasI AATT 6 cut(s) 49, 106, 112, 159, 320, 352
TatI WGTACW 1 cut(s) 53
TfiI GAWTC 2 cut(s) 422, 506
Tru1I TTAA 2 cut(s) 9, 483
Tru9I TTAA 2 cut(s) 9, 483
TscAI CASTG 2 cut(s) 139, 465
TseFI GTSAC 2 cut(s) 116, 536
TseI GCWGC 1 cut(s) 80
Tsp45I GTSAC 2 cut(s) 116, 536
TspDTI ATGAA 2 cut(s) 75, 285
TspGWI ACGGA 2 cut(s) 449, 534
TspRI CASTG 2 cut(s) 139, 465
Tth111I GACNNNGTC 1 cut(s) 287
VpaK11BI GGWCC 1 cut(s) 102
XceI RCATGY 2 cut(s) 458, 591
XmnI GAANNNNTTC 1 cut(s) 579
XspI CTAG 1 cut(s) 144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.