RH4BG255600

Topless-related protein

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_samantha
Unknown
OG0013792
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG255600.1

Sequence Viewer

Length: 3360 bp
ATGTCATCTCTGAGCAGAGAGCTGGTTTTCCTGATCCTTCAATTTCTGGAGGAGGAGAAATTCAAGGAGGCTGTACATAGGCTCGAGCAAGAGTCTGGATTCTTCTTCAACATGAAGTACTTCGAGGAGAAAGCGTTGCAGGGAGAGTGGGACGAGGTGGAGAAGTACCTGTCCGGGTTCACCAAGGTGGACGAGAATCGCTACTCTATGAAGATCTTCTTCGAGGTCAGAAAGCAGAAGTATCTGGAAGCGCTGGACAGGCAAGACAGGGCGAAAGCTGTTGAGATACTGGTGAAGGATTTGAAGGTGTTTTCGACGTTTAATGAGGACTTGTACAAGGAAATTACTCATCTGTTGACTCTTGACAACTTCAGAGAAAATGAGCAGTTGTCCAAGTATGGTGATACTAAATCCGCTAGAAGCATAATGCTGGTAGAGCTGAAGAAATTGATAGAAGCAAATCCTCTATTCCGAGAGAAGCTGGTTTTCCCCACTTTGAAGGCGTCACGCTTGCGAACTTTAATCAATCAAAGTTTAAATTGGCAGCACCAGCTATGCAAAAACCCAAGGCCAAATCCTGACATTAAGACCTTATTCATGGACCACTCGTGTTCTCCTCCAAATGGAGCTCGTGCATCGACTCCAGTTACTCTTCCAGTTGCAGCTCTTACAAAGCCTTTAACTTATGCTCCTCTTGGAGCACATGGTGGACCTTTCCCGCCGGCTGCTGCTGCTGCAGCTGCCAATGCGAATGCTTTAGCAGGATGGATGTCCAATGCTAATCCGTCCTTGTCTATGCAATCAGCTCCGGTCTCTGCTTCACCCCTGCCTGTTCAACTTGGTCAAGTTTCTTCTTTAAAGCATCCAAGAACACCATCAACTGGTCTTGGAATGATTGATTATCAAGGCTCTGATCATGAGCAACTAATGAAACGCCTGCGGACTGCACAATCTGTTGATGAGGTTAGCTATCCTCCTCAACATGCTTCCTGGTCGCTAGATGACCTACCGAGAAATGTATCTTGTACACTTCGTCAGGGATCCACTGTTATGAGCATGGATTTCCACCCTTCTCATCACACATTACTTGCTGTTGGCTGTAGTAATGGAGAAGTTACTCTGTGGGAAGTTGGGTTACGAGAGAAGCTGGTGTCCAAGCCATTCAAAGTATGGGAAATGACTACTTGTTCTCTGACATTTCAGGCGGCTATTGTCAAAGATTCATCAATGTCTGTTAACCGTGTATCATGGAGTCCAGATGGGAACTTAATCGGTGTTGCATTTACGAAACATTTGGTTCATCTGTATGCTTATCAAGGGTCTAATGATCTACGCCAACATTTGGAGATTGATGCCCACAGTGGAGGTGTGAATGACTTGGCATTCTCTCATCCAAACAAGCAATTGTGCGTTGTAACATGTGGAGATGATAAGATGATAAAGGTTTGGGATCTGGCTGGAAGGAAGCTTTTCCACTTTGAAGGTCATGAAGCACCTGTTTACTCGGTTTGCCCCCACCAGAAGGAGAATATTCAGTTTATATTTTCAACTGCTGTTGATGGAAAAATAAAAGCTTGGCTGTATGACAATGTGGGCTCCAGGGTTGACTATGATGCTCCAGGGCACTGGTGCACCACAATGCTGTACAGTGATGATGGAAATAGATTGTTCTCTTGTGGGACTAGTAAAGATGGTGACTCCTTCCTGGTTGAATGGAATGAAAGTGAAGGGGCAATAAAGAGGACCTATAGTGGGTTCAGAAAGAAATCTAATGGCATCGCTCAATTTGATACCACAAGAAATCACTTTTTGGCTGTTGGTGAAGATAGCCAGATAAAATTTTGGGATATGGATAATACAAATGTTCTTCTCAGTATAGAAGCTGATGGTGGACTACCACCTGTTCCTCGCTTGAGATTCAATAAGGAAGGGAATCTGCTTGTTGTTACCACGGCTGACAATGGATTCAAGGTTCTTGCAAATGCTGATGGTCTTAGATCCTTAAGAGCAATTGAAAATCGATCTTATGAAGCATCAAGAACACCTATTGAGATGAAGGTATCTACTTCGGCCATGGTTCCAAATATCAATCCTGCCATTAATAAAGTAGATCGTATGGATGCAAGCTCTCCTGCCAGACCTACTCAGATTCTTAATGGAGTTGATCCTATGGCAAGAAGTGTAGAAAAGCGAAGACTATTAGATGATGCATCCGAGAAAAATAAACGTTGGGAACTGGCAGAGATTGTTGATCCTGTTCACTGTCGAGTGGCTACGATGCCAGATAGTAAAGATCCTGCTAACAAGGTTGCTCGACTTCTGTACACAAATTCTGGTGCCGGCATTCTGGCTCTTGGTTCAAATGGGGTCCAGAAGCTATGGAAGTGGAGAAGCAGTGAACAAAATCCAAATGGAAAGGCCACTGCTAGTGTCGTTCCGCAACATTGGCAACCCAACAATGGTCTTTTGATGACTAATGATGTCCCCGAAAACTGTGAGGAAGCAGTTCCATGTATTGCACTCTCAAAGAATGACTCCTATGTGATGTCGGCATGTGGCGGAAAAGTGTCTCTGTTCAATATGATGACATTTAAGGTAATGACAACTTTCATGTCACCTCCACCAGCCTCAACCTTCCTAGCATTTCATCCTCTGGATAATAACATCATAGCAATTGGAACAGAAGATTCAACTATCCACATTTACAATGTCAAATCAAAACTGAAAGGCCACCAGAAACACATTACTGGATTAGCGTTTTCTGTTAACCTTAAGATCATGGTTTCATCAGGTGCTGATGCTCAACTATGCTTTTGGAACATGGATACGTGGGAGAAGAGGAAGTCGCTGACAATTCAACTGCCAGCCGGAAAGGCGCCTGTTGGTGACACACAAGTGCAATTCTATTCTGATCAAACTCGGTTGTTGGTATGCCATGAGACTCAACTAGCACTATATGATGCTGCCAAAGCAGAATGCATTCGACAGTGGATGCCGCAAGATGTACTGCCTGCACCAATAACATGTGCAGCATATTCCCCCAATAGTCAACTAGTCTATGCGGGTTTTACTGATGGCAATATTGGAGTATTTGATGCTGATAGTCTGAAATTAAGATGTCGTATTTCCATGTCAGTGTACTTGGCACAAGCATCCCCAAGCAGCCAAACTGTGTACCCGCTAGCTCTTACAGCACATCTGCAGGAGCCATATCAGTTCGCCGTTGGACTGACAGATGGATCTGTTAAAGTTATAGAGCCCTCAGACGCTGAGGGGAAGTGGGGAGTTACAGTGCCTGTCGATAACGGAGCACAGAACGGCTGGACAGCAACATCTTCGTCTAACAATCCTGCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

1119

Amino Acids

124.01

Weight (kDa)

6.67

Isoelectric Point (pI)

43.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH_TPL PF17814 5 - 34 3.5e-11 LisH-like dimerisation domain
TPR1-like_2nd PF21889 37 - 161 9.4e-62 TPR1-like, CTLH-containing domain
zf_topless PF21359 164 - 204 2.8e-25 TOPLESS, zinc finger domain
Beta-prop_EML_2 PF23414 331 - 467 6.4e-08 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd PF25172 348 - 563 5.8e-11 WDR3 second beta-propeller domain
Beta-prop_RIG_2nd PF23775 358 - 499 6.3e-07 RIG second beta-propeller
Beta-prop_WDR3_1st PF25173 389 - 526 6e-14 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 389 - 529 3.3e-06 WDR36/Utp21 first beta-propeller
WD40_Prp19 PF24814 391 - 491 8.3e-09 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 403 - 560 3.8e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 407 - 622 4.2e-21 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 410 - 663 1.5e-20 THOC3 beta-propeller domain
WDR55 PF24796 411 - 526 5e-08 WDR55
Beta-prop_EML PF23409 448 - 652 7.4e-08 Echinoderm microtubule-associated protein first beta-propeller
WD40 PF00400 449 - 484 1.8e-06 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 457 - 529 3.7e-08 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 464 - 566 2.7e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 466 - 657 9.4e-10 Prp19 WD40 domain
WDR55 PF24796 498 - 658 5e-07 WDR55
Beta-prop_WDR3_1st PF25173 517 - 658 2.4e-07 WDR3 first beta-propeller domain
WDR55 PF24796 830 - 942 2.6e-06 WDR55
Beta-prop_WDR3_1st PF25173 830 - 950 3.3e-13 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 831 - 951 6.9e-09 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 831 - 949 1.9e-11 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 832 - 1037 4.6e-17 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 832 - 1035 6.7e-19 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 833 - 942 6.9e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 836 - 949 3.4e-11 WDR3 second beta-propeller domain
Beta-prop_IFT140_1st PF23383 849 - 943 3.3e-06 IFT140 first beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 853 - 958 2e-07 WDR36/Utp21 first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 888 - 1037 5.8e-07 WDR36/Utp21 second beta-propeller domain
WD40_Prp19 PF24814 905 - 1039 1e-09 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 911 - 1041 3.1e-07 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013792)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G16830
fragaria_vesca FvH4_4g18910 FvH4_4g18910
malus_domestica MD13G1162100.v1.1 MD16G1161900.v1.1
prunus_persica Prupe.1G129400_v2.0.a1
pyrus_communis pycom13g13830
rosa_chinensis RchiOBHm_Chr4g0423811
rosa_laevigata RLG00000007530
rosa_multiflora Rmu_sc0003369.1_g000018
rosa_roxburghii Rroxscaffold_5G00365920
rosa_rugosa Rorug04G0190900
rosa_samantha Rh4AG250300 Rh4BG255600 Rh4CG263700 Rh4DG250000
rosa_wichuraiana Rw4G021430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 2336, 2875
AccB7I CCANNNNNTGG 2 cut(s) 881, 1342
AciI CCGC 9 cut(s) 414, 719, 940, 1205, 2438, 2559, 2996, 3062, 3179
AclI AACGTT 1 cut(s) 2227
AclWI GGATC 9 cut(s) 28, 1035, 1048, 1458, 1992, 2159, 2246, 2288, 3247
AcoI YGGCCR 1 cut(s) 2070
AcsI RAATTY 3 cut(s) 59, 1838, 2329
AcuI CTGAAG 2 cut(s) 355, 461
AcyI GRCGYC 2 cut(s) 503, 2876
AdeI CACNNNGTG 1 cut(s) 707
AfeI AGCGCT 1 cut(s) 252
AfiI CCNNNNNNNGG 6 cut(s) 623, 881, 1342, 1522, 1752, 2459
AflII CTTAAG 2 cut(s) 2002, 2771
AflIII ACRYGT 2 cut(s) 1418, 3023
AhlI ACTAGT 2 cut(s) 1682, 3052
AjnI CCWGG 4 cut(s) 989, 1598, 1618, 1704
AleI CACNNNNGTG 2 cut(s) 185, 2894
AloI GAACNNNNNNTCC 2 cut(s) 1956, 1988
Alw21I GWGCWC 4 cut(s) 631, 703, 1634, 3313
Alw26I GTCTC 3 cut(s) 817, 2574, 2933
Alw44I GTGCAC 1 cut(s) 1630
AlwI GGATC 9 cut(s) 28, 1035, 1048, 1458, 1992, 2159, 2246, 2288, 3247
AlwNI CAGNNNCTG 3 cut(s) 2795, 3269, 3296
Ama87I CYCGRG 1 cut(s) 83
Aor51HI AGCGCT 1 cut(s) 252
AoxI GGCC 4 cut(s) 569, 2070, 2418, 2728
ApaLI GTGCAC 1 cut(s) 1630
ApoI RAATTY 3 cut(s) 59, 1838, 2329
AseI ATTAAT 1 cut(s) 2100
Asp700I GAANNNNTTC 5 cut(s) 119, 215, 1469, 2505, 2979
AspLEI GCGC 2 cut(s) 253, 2878
AspS9I GGNCC 4 cut(s) 601, 710, 1743, 2368
AsuC2I CCSGG 1 cut(s) 175
AsuHPI GGTGA 8 cut(s) 172, 304, 413, 813, 1706, 1832, 2607, 2897
AsuNHI GCTAGC 1 cut(s) 3181
AvaI CYCGRG 1 cut(s) 83
AvaII GGWCC 4 cut(s) 601, 710, 1743, 2368
BaeGI GKGCMC 2 cut(s) 1626, 1634
BamHI GGATCC 1 cut(s) 1040
BanI GGYRCC 2 cut(s) 2336, 2875
BanII GRGCYC 3 cut(s) 631, 1598, 3261
BarI GAAGNNNNNNTAC 2 cut(s) 1119, 1151
BauI CACGAG 2 cut(s) 607, 630
BbsI GAAGAC 1 cut(s) 2200
Bbv12I GWGCWC 4 cut(s) 631, 703, 1634, 3313
BbvCI CCTCAGC 1 cut(s) 3270
BceAI ACGGC 3 cut(s) 1968, 3206, 3334
BcgI CGANNNNNNTGC 2 cut(s) 3318, 3352
BciT130I CCWGG 4 cut(s) 991, 1600, 1620, 1706
BciVI GTATCC 1 cut(s) 2818
BclI TGATCA 2 cut(s) 913, 2911
BcnI CCSGG 1 cut(s) 175
BcoDI GTCTC 3 cut(s) 817, 2574, 2933
BcuI ACTAGT 2 cut(s) 1682, 3052
BfaI CTAG 8 cut(s) 417, 998, 1683, 2427, 2639, 2948, 3053, 3182
BfmI CTRYAG 4 cut(s) 735, 1099, 1747, 3200
BfoI RGCGCY 2 cut(s) 254, 2879
BfrI CTTAAG 2 cut(s) 2002, 2771
BfuI GTATCC 1 cut(s) 2818
BglI GCCNNNNNGGC 1 cut(s) 2873
BglII AGATCT 1 cut(s) 213
BmcAI AGTACT 1 cut(s) 119
Bme1390I CCNGG 5 cut(s) 175, 991, 1600, 1620, 1706
Bme18I GGWCC 4 cut(s) 601, 710, 1743, 2368
BmeT110I CYCGRG 1 cut(s) 83
BmgT120I GGNCC 4 cut(s) 601, 710, 1743, 2368
BmiI GGNNCC 7 cut(s) 1042, 1597, 2079, 2338, 2369, 2877, 3207
BmrFI CCNGG 5 cut(s) 175, 991, 1600, 1620, 1706
BmtI GCTAGC 1 cut(s) 3185
BpiI GAAGAC 1 cut(s) 2200
BpmI CTGGAG 4 cut(s) 68, 627, 1582, 1602
Bpu10I CCTNAGC 1 cut(s) 3270
BpuEI CTTGAG 1 cut(s) 1933
BpuMI CCSGG 1 cut(s) 175
Bsa29I ATCGAT 1 cut(s) 2020
BsaAI YACGTR 1 cut(s) 2829
BsaHI GRCGYC 2 cut(s) 503, 2876
BsaI GGTCTC 1 cut(s) 817
BsaJI CCNNGG 6 cut(s) 183, 566, 1599, 1619, 1950, 2073
BsaWI WCCGGW 1 cut(s) 808
BsaXI ACNNNNNCTCC 8 cut(s) 119, 149, 673, 703, 2380, 2410, 3276, 3306
Bsc4I CCNNNNNNNGG 6 cut(s) 623, 881, 1342, 1522, 1752, 2459
Bse118I RCCGGY 2 cut(s) 721, 2339
Bse1I ACTGG 7 cut(s) 294, 644, 656, 886, 1631, 2241, 2752
BseBI CCWGG 4 cut(s) 991, 1600, 1620, 1706
BseCI ATCGAT 1 cut(s) 2020
BseDI CCNNGG 6 cut(s) 183, 566, 1599, 1619, 1950, 2073
BseGI GGATG 9 cut(s) 770, 774, 862, 1390, 2125, 2210, 2647, 2997, 3152
BseLI CCNNNNNNNGG 6 cut(s) 623, 881, 1342, 1522, 1752, 2459
BseMII CTCAG 4 cut(s) 1885, 2159, 3261, 3276
BseNI ACTGG 7 cut(s) 294, 644, 656, 886, 1631, 2241, 2752
BseRI GAGGAG 6 cut(s) 65, 68, 140, 606, 681, 966
BseSI GKGCMC 2 cut(s) 1626, 1634
BsgI GTGCAG 3 cut(s) 930, 2997, 3048
BshFI GGCC 4 cut(s) 571, 2072, 2420, 2730
BshNI GGYRCC 2 cut(s) 2336, 2875
BshVI ATCGAT 1 cut(s) 2020
BsiHKAI GWGCWC 4 cut(s) 631, 703, 1634, 3313
BsiHKCI CYCGRG 1 cut(s) 83
BsiSI CCGG 5 cut(s) 174, 722, 809, 2340, 2868
BslFI GGGAC 3 cut(s) 164, 1693, 2468
BslI CCNNNNNNNGG 6 cut(s) 623, 881, 1342, 1522, 1752, 2459
BsmAI GTCTC 3 cut(s) 817, 2574, 2933
BsmFI GGGAC 3 cut(s) 164, 1693, 2468
BsmI GAATGC 5 cut(s) 757, 1382, 2343, 2979, 2981
BsnI GGCC 4 cut(s) 571, 2072, 2420, 2730
Bso31I GGTCTC 1 cut(s) 817
BsoBI CYCGRG 1 cut(s) 83
Bsp1286I GDGCHC 7 cut(s) 631, 703, 1598, 1626, 1634, 3261, 3313
Bsp1407I TGTACA 5 cut(s) 73, 333, 1025, 1644, 2322
Bsp19I CCATGG 1 cut(s) 2073
BspACI CCGC 9 cut(s) 414, 719, 940, 1205, 2438, 2559, 2996, 3062, 3179
BspANI GGCC 4 cut(s) 571, 2072, 2420, 2730
BspCNI CTCAG 4 cut(s) 1884, 2158, 3262, 3275
BspDI ATCGAT 1 cut(s) 2020
BspHI TCATGA 2 cut(s) 916, 1486
BspLI GGNNCC 7 cut(s) 1042, 1597, 2079, 2338, 2369, 2877, 3207
BspMAI CTGCAG 2 cut(s) 739, 3204
BspOI GCTAGC 1 cut(s) 3185
BspPI GGATC 9 cut(s) 28, 1035, 1048, 1458, 1992, 2159, 2246, 2288, 3247
BspT107I GGYRCC 2 cut(s) 2336, 2875
BspTI CTTAAG 2 cut(s) 2002, 2771
BspTNI GGTCTC 1 cut(s) 817
BsrFI RCCGGY 2 cut(s) 721, 2339
BsrGI TGTACA 5 cut(s) 73, 333, 1025, 1644, 2322
BsrI ACTGG 7 cut(s) 294, 644, 656, 886, 1631, 2241, 2752
BssAI RCCGGY 2 cut(s) 721, 2339
BssECI CCNNGG 6 cut(s) 183, 566, 1599, 1619, 1950, 2073
BssNI GRCGYC 2 cut(s) 503, 2876
BssSI CACGAG 2 cut(s) 607, 630
BssT1I CCWWGG 3 cut(s) 183, 566, 2073
Bst2BI CACGAG 2 cut(s) 607, 630
Bst2UI CCWGG 4 cut(s) 991, 1600, 1620, 1706
Bst4CI ACNGT 9 cut(s) 1048, 1241, 1361, 1649, 2264, 2495, 2988, 3172, 3292
Bst6I CTCTTC 2 cut(s) 657, 2831
BstACI GRCGYC 2 cut(s) 503, 2876
BstAFI CTTAAG 2 cut(s) 2002, 2771
BstAUI TGTACA 5 cut(s) 73, 333, 1025, 1644, 2322
BstBAI YACGTR 1 cut(s) 2829
BstC8I GCNNGC 8 cut(s) 512, 723, 938, 2125, 2341, 2865, 3012, 3183
BstDEI CTNAG 6 cut(s) 11, 1871, 1994, 2145, 3262, 3270
BstDSI CCRYGG 2 cut(s) 1950, 2073
BstF5I GGATG 9 cut(s) 770, 774, 862, 1390, 2125, 2210, 2647, 2997, 3152
BstH2I RGCGCY 2 cut(s) 254, 2879
BstHHI GCGC 2 cut(s) 253, 2878
BstMAI GTCTC 3 cut(s) 817, 2574, 2933
BstNI CCWGG 4 cut(s) 991, 1600, 1620, 1706
BstNSI RCATGY 4 cut(s) 986, 1422, 2556, 3027
BstSCI CCNGG 5 cut(s) 173, 989, 1598, 1618, 1704
BstSFI CTRYAG 4 cut(s) 735, 1099, 1747, 3200
BstSLI GKGCMC 2 cut(s) 1626, 1634
BstV2I GAAGAC 1 cut(s) 2200
BstX2I RGATCY 6 cut(s) 213, 1040, 1450, 1997, 2293, 3239
BstXI CCANNNNNNTGG 1 cut(s) 1626
BstYI RGATCY 6 cut(s) 213, 1040, 1450, 1997, 2293, 3239
Bsu15I ATCGAT 1 cut(s) 2020
BsuI GTATCC 1 cut(s) 2818
BsuRI GGCC 4 cut(s) 571, 2072, 2420, 2730
BsuTUI ATCGAT 1 cut(s) 2020
BtgI CCRYGG 2 cut(s) 1950, 2073
BtgZI GCGATG 1 cut(s) 1762
BtsCI GGATG 9 cut(s) 770, 774, 862, 1390, 2125, 2210, 2647, 2997, 3152
BtsI GCAGTG 2 cut(s) 2401, 2421
Cac8I GCNNGC 8 cut(s) 512, 723, 938, 2125, 2341, 2865, 3012, 3183
CaiI CAGNNNCTG 3 cut(s) 2795, 3269, 3296
CciI TCATGA 2 cut(s) 916, 1486
CfoI GCGC 2 cut(s) 253, 2878
Cfr10I RCCGGY 2 cut(s) 721, 2339
Cfr13I GGNCC 4 cut(s) 601, 710, 1743, 2368
ClaI ATCGAT 1 cut(s) 2020
CseI GACGC 2 cut(s) 492, 3275
DdeI CTNAG 6 cut(s) 11, 1871, 1994, 2145, 3262, 3270
DinI GGCGCC 1 cut(s) 2877
DraI TTTAAA 2 cut(s) 537, 858
DraIII CACNNNGTG 1 cut(s) 707
EaeI YGGCCR 1 cut(s) 2070
Eam1104I CTCTTC 2 cut(s) 657, 2831
EarI CTCTTC 2 cut(s) 657, 2831
EciI GGCGGA 1 cut(s) 2574
Ecl136II GAGCTC 1 cut(s) 629
Eco130I CCWWGG 3 cut(s) 183, 566, 2073
Eco24I GRGCYC 3 cut(s) 631, 1598, 3261
Eco31I GGTCTC 1 cut(s) 817
Eco47I GGWCC 4 cut(s) 601, 710, 1743, 2368
Eco47III AGCGCT 1 cut(s) 252
Eco53kI GAGCTC 1 cut(s) 629
Eco57I CTGAAG 2 cut(s) 355, 461
Eco88I CYCGRG 1 cut(s) 83
EcoICRI GAGCTC 1 cut(s) 629
EcoO109I RGGNCCY 1 cut(s) 1743
EcoRII CCWGG 4 cut(s) 989, 1598, 1618, 1704
EcoT14I CCWWGG 3 cut(s) 183, 566, 2073
EcoT22I ATGCAT 2 cut(s) 2212, 2981
EcoT38I GRGCYC 3 cut(s) 631, 1598, 3261
EgeI GGCGCC 1 cut(s) 2877
EheI GGCGCC 1 cut(s) 2877
ErhI CCWWGG 3 cut(s) 183, 566, 2073
FalI AAGNNNNNCTT 2 cut(s) 203, 235
FaqI GGGAC 3 cut(s) 164, 1693, 2468
FauI CCCGC 3 cut(s) 726, 3055, 3186
FbaI TGATCA 2 cut(s) 913, 2911
FokI GGATG 9 cut(s) 777, 781, 849, 1377, 2132, 2197, 2634, 3004, 3139
FriOI GRGCYC 3 cut(s) 631, 1598, 3261
FspBI CTAG 8 cut(s) 417, 998, 1683, 2427, 2639, 2948, 3053, 3182
GlaI GCGC 2 cut(s) 252, 2877
GsuI CTGGAG 4 cut(s) 68, 627, 1582, 1602
HaeII RGCGCY 2 cut(s) 254, 2879
HaeIII GGCC 4 cut(s) 571, 2072, 2420, 2730
HapII CCGG 5 cut(s) 174, 722, 809, 2340, 2868
HgaI GACGC 2 cut(s) 492, 3275
HhaI GCGC 2 cut(s) 253, 2878
Hin1I GRCGYC 2 cut(s) 503, 2876
Hin6I GCGC 2 cut(s) 251, 2876
HinP1I GCGC 2 cut(s) 251, 2876
HincII GTYRAC 5 cut(s) 357, 1237, 1606, 2767, 3050
HindII GTYRAC 5 cut(s) 357, 1237, 1606, 2767, 3050
HindIII AAGCTT 2 cut(s) 1466, 1572
HpaI GTTAAC 2 cut(s) 1237, 2767
HpaII CCGG 5 cut(s) 174, 722, 809, 2340, 2868
HphI GGTGA 8 cut(s) 172, 304, 413, 813, 1706, 1832, 2607, 2897
Hpy99I CGWCG 1 cut(s) 319
HpyCH4III ACNGT 9 cut(s) 1048, 1241, 1361, 1649, 2264, 2495, 2988, 3172, 3292
HpyCH4IV ACGT 3 cut(s) 317, 2227, 2828
HpyF3I CTNAG 6 cut(s) 11, 1871, 1994, 2145, 3262, 3270
HpySE526I ACGT 3 cut(s) 317, 2227, 2828
Hsp92I GRCGYC 2 cut(s) 503, 2876
HspAI GCGC 2 cut(s) 251, 2876
KasI GGCGCC 1 cut(s) 2875
KroI GCCGGC 2 cut(s) 721, 2339
KroNI GCCGGC 2 cut(s) 723, 2341
Ksp22I TGATCA 2 cut(s) 913, 2911
KspAI GTTAAC 2 cut(s) 1237, 2767
LmnI GCTCC 8 cut(s) 626, 694, 698, 811, 1601, 1621, 3205, 3308
MaeI CTAG 8 cut(s) 417, 998, 1683, 2427, 2639, 2948, 3053, 3182
MaeII ACGT 3 cut(s) 317, 2227, 2828
MfeI CAATTG 3 cut(s) 1403, 2010, 2673
MflI RGATCY 6 cut(s) 213, 1040, 1450, 1997, 2293, 3239
MhlI GDGCHC 7 cut(s) 631, 703, 1598, 1626, 1634, 3261, 3313
Mly113I GGCGCC 1 cut(s) 2876
MlyI GAGTC 7 cut(s) 101, 352, 634, 1261, 1691, 2528, 2935
MmeI TCCRAC 1 cut(s) 3205
Mph1103I ATGCAT 2 cut(s) 2212, 2981
MroNI GCCGGC 2 cut(s) 721, 2339
MroXI GAANNNNTTC 5 cut(s) 119, 215, 1469, 2505, 2979
MslI CAYNNNNRTG 6 cut(s) 185, 1049, 1305, 1637, 2894, 3134
MspA1I CMGCKG 1 cut(s) 740
MspCI CTTAAG 2 cut(s) 2002, 2771
MspI CCGG 5 cut(s) 174, 722, 809, 2340, 2868
MspR9I CCNGG 5 cut(s) 175, 991, 1600, 1620, 1706
MunI CAATTG 3 cut(s) 1403, 2010, 2673
Mva1269I GAATGC 5 cut(s) 757, 1382, 2343, 2979, 2981
MvaI CCWGG 4 cut(s) 991, 1600, 1620, 1706
NaeI GCCGGC 2 cut(s) 723, 2341
NarI GGCGCC 1 cut(s) 2876
NciI CCSGG 1 cut(s) 175
NcoI CCATGG 1 cut(s) 2073
NgoMIV GCCGGC 2 cut(s) 721, 2339
NheI GCTAGC 1 cut(s) 3181
NlaIV GGNNCC 7 cut(s) 1042, 1597, 2079, 2338, 2369, 2877, 3207
NmuCI GTSAC 4 cut(s) 504, 1694, 2613, 2885
NsiI ATGCAT 2 cut(s) 2212, 2981
NspI RCATGY 4 cut(s) 986, 1422, 2556, 3027
OliI CACNNNNGTG 2 cut(s) 185, 2894
PaeR7I CTCGAG 1 cut(s) 83
PagI TCATGA 2 cut(s) 916, 1486
PciI ACATGT 2 cut(s) 1418, 3023
PctI GAATGC 5 cut(s) 757, 1382, 2343, 2979, 2981
PdiI GCCGGC 2 cut(s) 723, 2341
PdmI GAANNNNTTC 5 cut(s) 119, 215, 1469, 2505, 2979
PfeI GAWTC 8 cut(s) 99, 196, 1220, 1917, 1933, 1965, 2149, 2687
PflMI CCANNNNNTGG 2 cut(s) 881, 1342
PleI GAGTC 7 cut(s) 100, 352, 634, 1260, 1691, 2528, 2935
PluTI GGCGCC 1 cut(s) 2879
PpsI GAGTC 7 cut(s) 100, 352, 634, 1260, 1691, 2528, 2935
Ppu21I YACGTR 1 cut(s) 2829
PpuMI RGGWCCY 1 cut(s) 1743
PscI ACATGT 2 cut(s) 1418, 3023
PshBI ATTAAT 1 cut(s) 2100
Psp124BI GAGCTC 1 cut(s) 631
Psp1406I AACGTT 1 cut(s) 2227
Psp5II RGGWCCY 1 cut(s) 1743
Psp6I CCWGG 4 cut(s) 989, 1598, 1618, 1704
PspGI CCWGG 4 cut(s) 989, 1598, 1618, 1704
PspN4I GGNNCC 7 cut(s) 1042, 1597, 2079, 2338, 2369, 2877, 3207
PspPI GGNCC 4 cut(s) 601, 710, 1743, 2368
PspPPI RGGWCCY 1 cut(s) 1743
PspXI VCTCGAGB 1 cut(s) 83
PstI CTGCAG 2 cut(s) 739, 3204
PstNI CAGNNNCTG 3 cut(s) 2795, 3269, 3296
PsuI RGATCY 6 cut(s) 213, 1040, 1450, 1997, 2293, 3239
PvuII CAGCTG 1 cut(s) 740
RseI CAYNNNNRTG 6 cut(s) 185, 1049, 1305, 1637, 2894, 3134
SacI GAGCTC 1 cut(s) 631
Sau96I GGNCC 4 cut(s) 601, 710, 1743, 2368
ScaI AGTACT 1 cut(s) 119
SchI GAGTC 7 cut(s) 101, 352, 634, 1261, 1691, 2528, 2935
ScrFI CCNGG 5 cut(s) 175, 991, 1600, 1620, 1706
SduI GDGCHC 7 cut(s) 631, 703, 1598, 1626, 1634, 3261, 3313
SfcI CTRYAG 4 cut(s) 735, 1099, 1747, 3200
SfoI GGCGCC 1 cut(s) 2877
Sfr274I CTCGAG 1 cut(s) 83
SinI GGWCC 4 cut(s) 601, 710, 1743, 2368
SlaI CTCGAG 1 cut(s) 83
SmiMI CAYNNNNRTG 6 cut(s) 185, 1049, 1305, 1637, 2894, 3134
SmlI CTYRAG 4 cut(s) 83, 1912, 2002, 2771
SmoI CTYRAG 4 cut(s) 83, 1912, 2002, 2771
SpeI ACTAGT 2 cut(s) 1682, 3052
SsiI CCGC 9 cut(s) 414, 719, 940, 1205, 2438, 2559, 2996, 3062, 3179
SspDI GGCGCC 1 cut(s) 2875
SspI AATATT 2 cut(s) 1531, 3082
SspMI CTAG 8 cut(s) 417, 998, 1683, 2427, 2639, 2948, 3053, 3182
SstI GAGCTC 1 cut(s) 631
StyD4I CCNGG 5 cut(s) 173, 989, 1598, 1618, 1704
StyI CCWWGG 3 cut(s) 183, 566, 2073
TaaI ACNGT 9 cut(s) 1048, 1241, 1361, 1649, 2264, 2495, 2988, 3172, 3292
TaiI ACGT 3 cut(s) 320, 2230, 2831
TatI WGTACW 8 cut(s) 73, 117, 333, 1025, 1644, 2322, 3004, 3138
TauI GCSGC 2 cut(s) 1208, 2998
TfiI GAWTC 8 cut(s) 99, 196, 1220, 1917, 1933, 1965, 2149, 2687
TseFI GTSAC 4 cut(s) 504, 1694, 2613, 2885
Tsp45I GTSAC 4 cut(s) 504, 1694, 2613, 2885
TspGWI ACGGA 2 cut(s) 774, 3321
Van91I CCANNNNNTGG 2 cut(s) 881, 1342
Vha464I CTTAAG 2 cut(s) 2002, 2771
VneI GTGCAC 1 cut(s) 1630
VpaK11BI GGWCC 4 cut(s) 601, 710, 1743, 2368
VspI ATTAAT 1 cut(s) 2100
XapI RAATTY 3 cut(s) 59, 1838, 2329
XceI RCATGY 4 cut(s) 986, 1422, 2556, 3027
XcmI CCANNNNNNNNNTGG 1 cut(s) 1167
XhoI CTCGAG 1 cut(s) 83
XmnI GAANNNNTTC 5 cut(s) 119, 215, 1469, 2505, 2979
XspI CTAG 8 cut(s) 417, 998, 1683, 2427, 2639, 2948, 3053, 3182
ZrmI AGTACT 1 cut(s) 119
Zsp2I ATGCAT 2 cut(s) 2212, 2981
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.