AT3G18050

gpi-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0012959
Physical Location & Seq
Forward (+)
6180621 .. 6182899
2279 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G18050.1

Sequence Viewer

Length: 1008 bp
ATGTTCCAACACGCGCCTTCTTCCATTTCCCTCATCATCATCTTCCTCTTCACCATTCTATCACCCGTTTCACCTGAACCGGTCACAGTCCAGCCATTCCGGGTCAAATCATCCCCACCCGCAACCATACCCGCTTTCCCGGAGCAATCCGATTTCTCCGGCTGCCCACTCGATTTACCAGAAGACCTCTTCCACGGCATCAAGTCAGCTTGCAGCGGCAAAAAGCTCCACAAAGGAAAATGTTGCCCCGTACTAGGCGCGTGGCTCTACTCTGCTTACTCAACCACTGCTCTAAGCCGTTCGATTTCCGCCGCCGCAAGAAACTCATCCTCCTCCTCCGCCGCGGTAACGACTCCTGAAGAAGATATGCCTTTGCTTCCCGATGACTCGGAGACTTGCGTTGACGGATTAGGGAAATCTCTGAGGCAGAGAGGGATTGAGCTCACTAGACCGAACGAGACTTGCGACGTCGTTTACTGCTACTGTGGAATCAGATTGCATCCGTTGAGCTGTTCCGAGGCTTTTAGAGTGAATGATGAAGGGAGACTCGTTGGAGACGAGAGAGTTGATAGATTAGAGACTGATTGTTTGAGTGGAAGCCACAACAATGCTGATGGATTCTCACCTCTTCTTGGTTGCAACAAGTGCTTGAACAGTCTCTATAAGCTAAATCCGAAGAAAACTTCAGGGACAAGAAACCCATCAAAGGAAGACCGAAACAGAACAGCAAAGATGCACAACAAAGACTGTGTCCTCATGGGTCTCACTTGGCTTCTCGCTAAGAACCGTACTGCTTATTTCCCCACTGTCACTTCTGTCCTCCGAGCCGTCATGCTGAACCACGATGGCGTGCCACGTTCATGTGCTCTCGGCAGCGACGGCATGCCTTTAGCCGTCGATTCTTCCGAATTCTCCAACGGCTCGCCAACTTCACTTCAGTATCCGCACCACTTGGTCCACTTCTTACTTTACAGCGTTATCACATTAGTCCTAATAAGGTCGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

335

Amino Acids

36.54

Weight (kDa)

7.52

Isoelectric Point (pI)

56.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPARK PF19160 53 - 236 6.5e-08 SPARK
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012959)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18050
fragaria_vesca FvH4_5g17140 FvH4_5g17140
malus_domestica MD06G1230000.v1.1 MD14G1234700.v1.1
prunus_persica Prupe.5G231000_v2.0.a1
pyrus_communis pycom06g20120 pycom14g19570
rosa_chinensis RchiOBHm_Chr7g0178651
rosa_laevigata RLG00000005426
rosa_multiflora Rmu_sc0002320.1_g000008
rosa_roxburghii Rroxscaffold_3G00274740
rosa_rugosa Rorug06G0418700
rosa_samantha Rh7AG017500 Rh7BG017100 Rh7CG017700 Rh7DG017700
rosa_wichuraiana Rw7G001420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 471
AccII CGCG 3 cut(s) 14, 260, 344
AcsI RAATTY 1 cut(s) 908
AcuI CTGAAG 3 cut(s) 378, 669, 920
AcyI GRCGYC 1 cut(s) 468
AfaI GTAC 2 cut(s) 252, 790
AfiI CCNNNNNNNGG 3 cut(s) 254, 632, 706
AgeI ACCGGT 1 cut(s) 79
AgsI TTSAA 1 cut(s) 652
AluBI AGCT 5 cut(s) 209, 226, 442, 510, 667
AluI AGCT 5 cut(s) 209, 226, 442, 510, 667
Alw21I GWGCWC 2 cut(s) 444, 868
Alw26I GTCTC 7 cut(s) 386, 452, 538, 549, 572, 662, 767
ApeKI GCWGC 3 cut(s) 162, 213, 873
ApoI RAATTY 1 cut(s) 908
ArsI GACNNNNNNTTYG 4 cut(s) 571, 603, 735, 767
AsiGI ACCGGT 1 cut(s) 79
AspLEI GCGC 2 cut(s) 16, 260
AspS9I GGNCC 1 cut(s) 955
AsuC2I CCSGG 2 cut(s) 101, 140
AsuHPI GGTGA 4 cut(s) 43, 54, 63, 615
AvaII GGWCC 1 cut(s) 955
BaeI ACNNNNGTAYC 2 cut(s) 923, 956
BanII GRGCYC 1 cut(s) 444
BbsI GAAGAC 2 cut(s) 189, 717
Bbv12I GWGCWC 2 cut(s) 444, 868
BbvI GCAGC 3 cut(s) 149, 225, 885
BccI CCATC 3 cut(s) 608, 709, 839
BceAI ACGGC 6 cut(s) 211, 282, 812, 878, 895, 934
BciVI GTATCC 1 cut(s) 951
BcnI CCSGG 2 cut(s) 101, 140
BcoDI GTCTC 7 cut(s) 386, 452, 538, 549, 572, 662, 767
BfaI CTAG 2 cut(s) 254, 447
BfuI GTATCC 1 cut(s) 951
BisI GCNGC 7 cut(s) 163, 214, 217, 312, 315, 342, 874
BlsI GCNGC 7 cut(s) 164, 215, 218, 313, 316, 343, 875
Bme1390I CCNGG 2 cut(s) 101, 140
Bme18I GGWCC 1 cut(s) 955
BmgT120I GGNCC 1 cut(s) 955
BmrFI CCNGG 2 cut(s) 101, 140
BmsI GCATC 3 cut(s) 207, 508, 723
BpiI GAAGAC 2 cut(s) 189, 717
BpuMI CCSGG 2 cut(s) 101, 140
BsaHI GRCGYC 1 cut(s) 468
BsaI GGTCTC 1 cut(s) 767
BsaJI CCNNGG 3 cut(s) 193, 342, 516
BsaWI WCCGGW 1 cut(s) 79
BsaXI ACNNNNNCTCC 2 cut(s) 314, 344
Bsc4I CCNNNNNNNGG 3 cut(s) 254, 632, 706
Bse118I RCCGGY 1 cut(s) 79
BseDI CCNNGG 3 cut(s) 193, 342, 516
BseGI GGATG 3 cut(s) 110, 326, 499
BseLI CCNNNNNNNGG 3 cut(s) 254, 632, 706
BseMII CTCAG 1 cut(s) 413
BseRI GAGGAG 2 cut(s) 322, 325
BseXI GCAGC 3 cut(s) 149, 225, 885
Bsh1236I CGCG 3 cut(s) 14, 260, 344
BshTI ACCGGT 1 cut(s) 79
BsiHKAI GWGCWC 2 cut(s) 444, 868
BsiSI CCGG 4 cut(s) 80, 100, 140, 159
BslFI GGGAC 1 cut(s) 703
BslI CCNNNNNNNGG 3 cut(s) 254, 632, 706
BsmAI GTCTC 7 cut(s) 386, 452, 538, 549, 572, 662, 767
BsmBI CGTCTC 1 cut(s) 549
BsmFI GGGAC 1 cut(s) 703
Bso31I GGTCTC 1 cut(s) 767
Bsp1286I GDGCHC 2 cut(s) 444, 868
BspCNI CTCAG 1 cut(s) 414
BspFNI CGCG 3 cut(s) 14, 260, 344
BspTNI GGTCTC 1 cut(s) 767
BsrFI RCCGGY 1 cut(s) 79
BssAI RCCGGY 1 cut(s) 79
BssECI CCNNGG 3 cut(s) 193, 342, 516
BssNI GRCGYC 1 cut(s) 468
Bst4CI ACNGT 6 cut(s) 88, 485, 656, 749, 788, 808
Bst6I CTCTTC 3 cut(s) 53, 194, 633
BstACI GRCGYC 1 cut(s) 468
BstAPI GCANNNNNTGC 1 cut(s) 645
BstC8I GCNNGC 4 cut(s) 211, 851, 884, 923
BstDEI CTNAG 3 cut(s) 293, 422, 780
BstDSI CCRYGG 2 cut(s) 193, 342
BstF5I GGATG 3 cut(s) 110, 326, 499
BstFNI CGCG 3 cut(s) 14, 260, 344
BstHHI GCGC 2 cut(s) 16, 260
BstMAI GTCTC 7 cut(s) 386, 452, 538, 549, 572, 662, 767
BstMWI GCNNNNNNNGC 2 cut(s) 645, 879
BstNSI RCATGY 1 cut(s) 886
BstSCI CCNGG 2 cut(s) 99, 138
BstUI CGCG 3 cut(s) 14, 260, 344
BstV1I GCAGC 3 cut(s) 149, 225, 885
BstV2I GAAGAC 2 cut(s) 189, 717
BsuI GTATCC 1 cut(s) 951
BtgI CCRYGG 2 cut(s) 193, 342
BtsCI GGATG 3 cut(s) 110, 326, 499
BtsI GCAGTG 1 cut(s) 285
BtsIMutI CAGTG 2 cut(s) 285, 804
Cac8I GCNNGC 4 cut(s) 211, 851, 884, 923
CfoI GCGC 2 cut(s) 16, 260
Cfr10I RCCGGY 1 cut(s) 79
Cfr13I GGNCC 1 cut(s) 955
Cfr42I CCGCGG 1 cut(s) 345
Csp6I GTAC 2 cut(s) 251, 789
CspAI ACCGGT 1 cut(s) 79
CviAII CATG 4 cut(s) 757, 832, 861, 883
CviQI GTAC 2 cut(s) 251, 789
DdeI CTNAG 3 cut(s) 293, 422, 780
Eam1104I CTCTTC 3 cut(s) 53, 194, 633
EarI CTCTTC 3 cut(s) 53, 194, 633
EciI GGCGGA 2 cut(s) 298, 328
Ecl136II GAGCTC 1 cut(s) 442
Eco24I GRGCYC 1 cut(s) 444
Eco31I GGTCTC 1 cut(s) 767
Eco47I GGWCC 1 cut(s) 955
Eco53kI GAGCTC 1 cut(s) 442
Eco57I CTGAAG 3 cut(s) 378, 669, 920
EcoICRI GAGCTC 1 cut(s) 442
EcoRI GAATTC 1 cut(s) 908
EcoT38I GRGCYC 1 cut(s) 444
Esp3I CGTCTC 1 cut(s) 549
FaeI CATG 4 cut(s) 760, 835, 864, 886
FaiI YATR 7 cut(s) 128, 368, 663, 758, 833, 862, 884
FaqI GGGAC 1 cut(s) 703
FatI CATG 4 cut(s) 756, 831, 860, 882
FauI CCCGC 2 cut(s) 127, 139
Fnu4HI GCNGC 7 cut(s) 163, 214, 217, 312, 315, 342, 874
FokI GGATG 3 cut(s) 97, 313, 486
FriOI GRGCYC 1 cut(s) 444
Fsp4HI GCNGC 7 cut(s) 163, 214, 217, 312, 315, 342, 874
FspBI CTAG 2 cut(s) 254, 447
GlaI GCGC 2 cut(s) 15, 259
GluI GCNGC 7 cut(s) 163, 214, 217, 312, 315, 342, 874
HapII CCGG 4 cut(s) 80, 100, 140, 159
HhaI GCGC 2 cut(s) 16, 260
Hin1I GRCGYC 1 cut(s) 468
Hin1II CATG 4 cut(s) 760, 835, 864, 886
Hin6I GCGC 2 cut(s) 14, 258
HinP1I GCGC 2 cut(s) 14, 258
HincII GTYRAC 1 cut(s) 403
HindII GTYRAC 1 cut(s) 403
HinfI GANTC 6 cut(s) 352, 386, 489, 546, 618, 899
HpaII CCGG 4 cut(s) 80, 100, 140, 159
HphI GGTGA 4 cut(s) 43, 54, 63, 615
Hpy166II GTNNAC 3 cut(s) 403, 475, 958
Hpy188I TCNGA 8 cut(s) 151, 391, 423, 494, 517, 675, 824, 907
Hpy188III TCNNGA 2 cut(s) 356, 380
Hpy8I GTNNAC 3 cut(s) 403, 475, 958
Hpy99I CGWCG 4 cut(s) 470, 473, 881, 899
HpyAV CCTTC 2 cut(s) 27, 533
HpyCH4III ACNGT 6 cut(s) 88, 485, 656, 749, 788, 808
HpyCH4IV ACGT 2 cut(s) 468, 856
HpyCH4V TGCA 4 cut(s) 213, 499, 639, 736
HpyF10VI GCNNNNNNNGC 2 cut(s) 645, 879
HpyF3I CTNAG 3 cut(s) 293, 422, 780
HpySE526I ACGT 2 cut(s) 468, 856
Hsp92I GRCGYC 1 cut(s) 468
Hsp92II CATG 4 cut(s) 760, 835, 864, 886
HspAI GCGC 2 cut(s) 14, 258
KspI CCGCGG 1 cut(s) 345
LmnI GCTCC 2 cut(s) 142, 231
LpnPI CCDG 9 cut(s) 87, 93, 104, 113, 153, 172, 192, 369, 672
Lsp1109I GCAGC 3 cut(s) 149, 225, 885
LweI GCATC 3 cut(s) 207, 508, 723
MaeI CTAG 2 cut(s) 254, 447
MaeII ACGT 2 cut(s) 468, 856
MaeIII GTNAC 3 cut(s) 82, 346, 808
MhlI GDGCHC 2 cut(s) 444, 868
MluCI AATT 1 cut(s) 908
MlyI GAGTC 3 cut(s) 346, 380, 540
MmeI TCCRAC 3 cut(s) 31, 532, 939
MslI CAYNNNNRTG 2 cut(s) 606, 859
MspA1I CMGCKG 2 cut(s) 216, 344
MspI CCGG 4 cut(s) 80, 100, 140, 159
MspR9I CCNGG 2 cut(s) 101, 140
MvnI CGCG 3 cut(s) 14, 260, 344
MwoI GCNNNNNNNGC 2 cut(s) 645, 879
NciI CCSGG 2 cut(s) 101, 140
NlaIII CATG 4 cut(s) 760, 835, 864, 886
NmeAIII GCCGAG 1 cut(s) 849
NmuCI GTSAC 2 cut(s) 82, 808
NspI RCATGY 1 cut(s) 886
PaeI GCATGC 1 cut(s) 886
PcsI WCGNNNNNNNCGW 3 cut(s) 462, 555, 903
PfeI GAWTC 3 cut(s) 489, 618, 899
PflFI GACNNNGTC 1 cut(s) 749
PfoI TCCNGGA 1 cut(s) 138
PinAI ACCGGT 1 cut(s) 79
PkrI GCNGC 7 cut(s) 164, 215, 218, 313, 316, 343, 875
PleI GAGTC 3 cut(s) 346, 380, 540
PpsI GAGTC 3 cut(s) 346, 380, 540
Psp124BI GAGCTC 1 cut(s) 444
PspPI GGNCC 1 cut(s) 955
PsyI GACNNNGTC 1 cut(s) 749
RsaI GTAC 2 cut(s) 252, 790
RsaNI GTAC 2 cut(s) 251, 789
RseI CAYNNNNRTG 2 cut(s) 606, 859
SacI GAGCTC 1 cut(s) 444
SacII CCGCGG 1 cut(s) 345
SatI GCNGC 7 cut(s) 163, 214, 217, 312, 315, 342, 874
Sau96I GGNCC 1 cut(s) 955
SchI GAGTC 3 cut(s) 346, 380, 540
ScrFI CCNGG 2 cut(s) 101, 140
SduI GDGCHC 2 cut(s) 444, 868
SfaNI GCATC 3 cut(s) 207, 508, 723
Sfr303I CCGCGG 1 cut(s) 345
SgrBI CCGCGG 1 cut(s) 345
SinI GGWCC 1 cut(s) 955
SmiMI CAYNNNNRTG 2 cut(s) 606, 859
SphI GCATGC 1 cut(s) 886
Sse9I AATT 1 cut(s) 908
SspMI CTAG 2 cut(s) 254, 447
SstI GAGCTC 1 cut(s) 444
StyD4I CCNGG 2 cut(s) 99, 138
TaaI ACNGT 6 cut(s) 88, 485, 656, 749, 788, 808
TaiI ACGT 2 cut(s) 471, 859
TaqI TCGA 3 cut(s) 171, 302, 897
TaqII GACCGA 2 cut(s) 466, 729
TasI AATT 1 cut(s) 908
TauI GCSGC 4 cut(s) 219, 314, 317, 344
TfiI GAWTC 3 cut(s) 489, 618, 899
TscAI CASTG 2 cut(s) 292, 811
TseFI GTSAC 2 cut(s) 82, 808
TseI GCWGC 3 cut(s) 162, 213, 873
Tsp45I GTSAC 2 cut(s) 82, 808
TspDTI ATGAA 2 cut(s) 552, 849
TspGWI ACGGA 2 cut(s) 420, 492
TspRI CASTG 2 cut(s) 292, 811
Tth111I GACNNNGTC 1 cut(s) 749
VpaK11BI GGWCC 1 cut(s) 955
XapI RAATTY 1 cut(s) 908
XceI RCATGY 1 cut(s) 886
XspI CTAG 2 cut(s) 254, 447
ZraI GACGTC 1 cut(s) 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.