pycom14g19570

GPI-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0012959
Physical Location & Seq
Reverse (-)
20844622 .. 20846315
1694 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g19570.1

Sequence Viewer

Length: 993 bp
ATGTTCTTCACCACCTTCTTCTTCCTCTGCATTCTCTTCCCTGCCTTCCCGGGTCTACCCAACCCGGACGCCGCTCCCACCCAGTCATTCCTCCCAAACCGAACTCCCTCCCCACCCGCTACAATCCCTGCATTCCCAGAACAATCCAACTTAGTCGGGTGCCCGCTGCACCTCCCCAATGAGCTCTTCCATGGCATAAAGTCCGCATGCTCCGCCACCAAAGGCGATGCCTCAGGTCACTTGCAGCGCAGTAGGTGCTGTCCTGCGCTGGCCTCGTGGCTCTACTTTGCCTACTCTGACACCGCTCTCGGCAGAGTAGGCAGGGTGGGCCCAGCAGTGGCCGGTCAGCCTTATGATCCGCCACTGCTGCCGGACGACACGGAAACTTGCGTTCAGGACTTGGGAAAGGCGCTGAAAAACAGAGGAATCGAGCTAATCAAGCCGAATGAGACCTGTGATGTTGTGTACTGCTATTGCGGGATAAGATTGCACCCCTTGAGCTGTCCCGAGGCGTTTTCGGAGAACGAAAGAGGGAAGCTTGTTGGGGATGAGAAGGTGAAGAAGTTGGAGAGAGATTGCTTGAGCAGCGGCGCCAATGGCAATGGTCTTGGTGGCTGCTCCAAATGCTTGCACAGTCTCTACAATCTTAACAAGAAGAAAACTTCCAATTCAAGCAAATTAGGGGACAGGACCACCAAAATGCACAACAAAGACTGTCAGCTGATGGGTCTCACTTGGCTCCTTGCCAAGAATCGGACGGCTTACATCCACACAGTTACATCCGTGTTTCGAGCTATCATGGTAAGCACCGACGGCTCCGATCCTCGGTCGTGCACGCTCAACAGTGACGGAATGCCTCTCGCTGTCGATTCTTCTGAAATTTCTGATCAGTCCTCGTCCAGCATCCTCGCAGCTTCCGTCTCGTTTTACCTTGTAATGTCACTTTCTTTGTTTTTTATGTTGTCGCTTGCCCTATGCTCCATCCAAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

331

Amino Acids

35.68

Weight (kDa)

7.85

Isoelectric Point (pI)

48.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPARK PF19160 52 - 239 2e-09 SPARK
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012959)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18050
fragaria_vesca FvH4_5g17140 FvH4_5g17140
malus_domestica MD06G1230000.v1.1 MD14G1234700.v1.1
prunus_persica Prupe.5G231000_v2.0.a1
pyrus_communis pycom06g20120 pycom14g19570
rosa_chinensis RchiOBHm_Chr7g0178651
rosa_laevigata RLG00000005426
rosa_multiflora Rmu_sc0002320.1_g000008
rosa_roxburghii Rroxscaffold_3G00274740
rosa_rugosa Rorug06G0418700
rosa_samantha Rh7AG017500 Rh7BG017100 Rh7CG017700 Rh7DG017700
rosa_wichuraiana Rw7G001420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 159, 590
AccBSI CCGCTC 2 cut(s) 74, 305
AccI GTMKAC 1 cut(s) 55
AciI CCGC 9 cut(s) 72, 117, 164, 204, 213, 303, 359, 477, 588
AclWI GGATC 2 cut(s) 350, 815
AcoI YGGCCR 1 cut(s) 339
AcsI RAATTY 1 cut(s) 879
AcyI GRCGYC 2 cut(s) 69, 591
AfaI GTAC 1 cut(s) 467
AfiI CCNNNNNNNGG 3 cut(s) 337, 753, 825
AgsI TTSAA 1 cut(s) 672
AjuI GAANNNNNNNTTGG 2 cut(s) 170, 202
AluBI AGCT 7 cut(s) 184, 433, 501, 538, 721, 794, 914
AluI AGCT 7 cut(s) 184, 433, 501, 538, 721, 794, 914
Alw21I GWGCWC 2 cut(s) 186, 836
Alw26I GTCTC 4 cut(s) 443, 641, 734, 925
Alw44I GTGCAC 1 cut(s) 832
AlwI GGATC 2 cut(s) 350, 815
Ama87I CYCGRG 2 cut(s) 49, 506
AoxI GGCC 3 cut(s) 270, 328, 339
ApaI GGGCCC 1 cut(s) 332
ApaLI GTGCAC 1 cut(s) 832
ApeKI GCWGC 6 cut(s) 166, 244, 367, 585, 615, 911
ApoI RAATTY 1 cut(s) 879
AspLEI GCGC 4 cut(s) 249, 268, 412, 593
AspS9I GGNCC 3 cut(s) 328, 329, 690
AsuC2I CCSGG 3 cut(s) 50, 51, 65
AsuHPI GGTGA 1 cut(s) 568
AvaI CYCGRG 2 cut(s) 49, 506
AvaII GGWCC 1 cut(s) 690
AxyI CCTNAGG 1 cut(s) 232
BaeGI GKGCMC 3 cut(s) 164, 332, 836
BanI GGYRCC 2 cut(s) 159, 590
BanII GRGCYC 2 cut(s) 186, 332
BauI CACGAG 1 cut(s) 274
Bbv12I GWGCWC 2 cut(s) 186, 836
BbvI GCAGC 6 cut(s) 153, 256, 354, 597, 602, 923
BccI CCATC 2 cut(s) 718, 989
BceAI ACGGC 2 cut(s) 774, 829
BclI TGATCA 1 cut(s) 886
BcnI CCSGG 3 cut(s) 50, 51, 65
BcoDI GTCTC 4 cut(s) 443, 641, 734, 925
BfaI CTAG 1 cut(s) 991
BfoI RGCGCY 2 cut(s) 413, 594
BisI GCNGC 8 cut(s) 72, 167, 245, 368, 586, 589, 616, 912
BlsI GCNGC 8 cut(s) 73, 168, 246, 369, 587, 590, 617, 913
Bme1390I CCNGG 3 cut(s) 50, 51, 65
Bme18I GGWCC 1 cut(s) 690
BmeT110I CYCGRG 2 cut(s) 49, 506
BmgT120I GGNCC 3 cut(s) 328, 329, 690
BmiI GGNNCC 5 cut(s) 161, 330, 592, 740, 817
BmrFI CCNGG 3 cut(s) 50, 51, 65
BmrI ACTGGG 1 cut(s) 76
BmsI GCATC 2 cut(s) 217, 912
BmuI ACTGGG 1 cut(s) 76
BpuEI CTTGAG 2 cut(s) 517, 601
BpuMI CCSGG 3 cut(s) 50, 51, 65
BsaHI GRCGYC 2 cut(s) 69, 591
BsaI GGTCTC 2 cut(s) 443, 734
BsaJI CCNNGG 4 cut(s) 49, 190, 507, 824
Bsc4I CCNNNNNNNGG 3 cut(s) 337, 753, 825
Bse118I RCCGGY 1 cut(s) 341
Bse1I ACTGG 1 cut(s) 82
Bse21I CCTNAGG 1 cut(s) 232
Bse3DI GCAATG 1 cut(s) 607
BseDI CCNNGG 4 cut(s) 49, 190, 507, 824
BseGI GGATG 5 cut(s) 553, 765, 779, 903, 981
BseLI CCNNNNNNNGG 3 cut(s) 337, 753, 825
BseMI GCAATG 1 cut(s) 607
BseMII CTCAG 1 cut(s) 246
BseNI ACTGG 1 cut(s) 82
BseSI GKGCMC 3 cut(s) 164, 332, 836
BseXI GCAGC 6 cut(s) 153, 256, 354, 597, 602, 923
BseYI CCCAGC 1 cut(s) 331
BsgI GTGCAG 1 cut(s) 152
Bsh1285I CGRYCG 1 cut(s) 830
BshFI GGCC 3 cut(s) 272, 330, 341
BshNI GGYRCC 2 cut(s) 159, 590
BsiEI CGRYCG 1 cut(s) 830
BsiHKAI GWGCWC 2 cut(s) 186, 836
BsiHKCI CYCGRG 2 cut(s) 49, 506
BsiSI CCGG 4 cut(s) 50, 65, 342, 371
BslFI GGGAC 2 cut(s) 489, 698
BslI CCNNNNNNNGG 3 cut(s) 337, 753, 825
BsmAI GTCTC 4 cut(s) 443, 641, 734, 925
BsmBI CGTCTC 1 cut(s) 925
BsmFI GGGAC 2 cut(s) 489, 698
BsmI GAATGC 3 cut(s) 30, 131, 858
BsnI GGCC 3 cut(s) 272, 330, 341
Bso31I GGTCTC 2 cut(s) 443, 734
BsoBI CYCGRG 2 cut(s) 49, 506
Bsp120I GGGCCC 1 cut(s) 328
Bsp1286I GDGCHC 4 cut(s) 164, 186, 332, 836
Bsp143I GATC 3 cut(s) 355, 820, 886
Bsp19I CCATGG 1 cut(s) 190
BspACI CCGC 9 cut(s) 72, 117, 164, 204, 213, 303, 359, 477, 588
BspANI GGCC 3 cut(s) 272, 330, 341
BspCNI CTCAG 1 cut(s) 245
BspLI GGNNCC 5 cut(s) 161, 330, 592, 740, 817
BspPI GGATC 2 cut(s) 350, 815
BspQI GCTCTTC 1 cut(s) 191
BspT107I GGYRCC 2 cut(s) 159, 590
BspTNI GGTCTC 2 cut(s) 443, 734
BsrBI CCGCTC 2 cut(s) 74, 305
BsrDI GCAATG 1 cut(s) 607
BsrFI RCCGGY 1 cut(s) 341
BsrI ACTGG 1 cut(s) 82
BssAI RCCGGY 1 cut(s) 341
BssECI CCNNGG 4 cut(s) 49, 190, 507, 824
BssMI GATC 3 cut(s) 355, 820, 886
BssNI GRCGYC 2 cut(s) 69, 591
BssSI CACGAG 1 cut(s) 274
BssT1I CCWWGG 1 cut(s) 190
Bst2BI CACGAG 1 cut(s) 274
Bst4CI ACNGT 4 cut(s) 635, 716, 775, 845
Bst6I CTCTTC 2 cut(s) 41, 191
BstACI GRCGYC 2 cut(s) 69, 591
BstAPI GCANNNNNTGC 1 cut(s) 255
BstC8I GCNNGC 6 cut(s) 164, 208, 270, 629, 836, 969
BstDEI CTNAG 2 cut(s) 151, 232
BstDSI CCRYGG 1 cut(s) 190
BstF5I GGATG 5 cut(s) 553, 765, 779, 903, 981
BstH2I RGCGCY 2 cut(s) 413, 594
BstHHI GCGC 4 cut(s) 249, 268, 412, 593
BstKTI GATC 3 cut(s) 358, 823, 889
BstMAI GTCTC 4 cut(s) 443, 641, 734, 925
BstMBI GATC 3 cut(s) 355, 820, 886
BstMCI CGRYCG 1 cut(s) 830
BstNSI RCATGY 1 cut(s) 210
BstSCI CCNGG 3 cut(s) 48, 49, 63
BstSLI GKGCMC 3 cut(s) 164, 332, 836
BstV1I GCAGC 6 cut(s) 153, 256, 354, 597, 602, 923
Bsu36I CCTNAGG 1 cut(s) 232
BsuRI GGCC 3 cut(s) 272, 330, 341
BtgI CCRYGG 1 cut(s) 190
BtgZI GCGATG 1 cut(s) 240
BtsCI GGATG 5 cut(s) 553, 765, 779, 903, 981
BtsI GCAGTG 2 cut(s) 342, 362
BtsIMutI CAGTG 3 cut(s) 342, 362, 850
Cac8I GCNNGC 6 cut(s) 164, 208, 270, 629, 836, 969
CfoI GCGC 4 cut(s) 249, 268, 412, 593
Cfr10I RCCGGY 1 cut(s) 341
Cfr13I GGNCC 3 cut(s) 328, 329, 690
Cfr9I CCCGGG 1 cut(s) 49
CseI GACGC 1 cut(s) 77
Csp6I GTAC 1 cut(s) 466
CviAII CATG 3 cut(s) 191, 207, 799
CviQI GTAC 1 cut(s) 466
DdeI CTNAG 2 cut(s) 151, 232
DinI GGCGCC 1 cut(s) 592
DpnI GATC 3 cut(s) 357, 822, 888
DpnII GATC 3 cut(s) 355, 820, 886
EaeI YGGCCR 1 cut(s) 339
Eam1104I CTCTTC 2 cut(s) 41, 191
EarI CTCTTC 2 cut(s) 41, 191
EciI GGCGGA 2 cut(s) 202, 348
Ecl136II GAGCTC 1 cut(s) 184
Eco130I CCWWGG 1 cut(s) 190
Eco24I GRGCYC 2 cut(s) 186, 332
Eco31I GGTCTC 2 cut(s) 443, 734
Eco47I GGWCC 1 cut(s) 690
Eco53kI GAGCTC 1 cut(s) 184
Eco81I CCTNAGG 1 cut(s) 232
Eco88I CYCGRG 2 cut(s) 49, 506
EcoICRI GAGCTC 1 cut(s) 184
EcoT14I CCWWGG 1 cut(s) 190
EcoT38I GRGCYC 2 cut(s) 186, 332
EgeI GGCGCC 1 cut(s) 592
EheI GGCGCC 1 cut(s) 592
ErhI CCWWGG 1 cut(s) 190
Esp3I CGTCTC 1 cut(s) 925
FaeI CATG 3 cut(s) 194, 210, 802
FaiI YATR 7 cut(s) 192, 197, 208, 354, 800, 959, 976
FaqI GGGAC 2 cut(s) 489, 698
FatI CATG 3 cut(s) 190, 206, 798
FauI CCCGC 3 cut(s) 124, 171, 470
FbaI TGATCA 1 cut(s) 886
FblI GTMKAC 1 cut(s) 55
Fnu4HI GCNGC 8 cut(s) 72, 167, 245, 368, 586, 589, 616, 912
FokI GGATG 5 cut(s) 560, 752, 766, 890, 968
FriOI GRGCYC 2 cut(s) 186, 332
Fsp4HI GCNGC 8 cut(s) 72, 167, 245, 368, 586, 589, 616, 912
FspBI CTAG 1 cut(s) 991
GlaI GCGC 4 cut(s) 248, 267, 411, 592
GluI GCNGC 8 cut(s) 72, 167, 245, 368, 586, 589, 616, 912
GsaI CCCAGC 1 cut(s) 335
HaeII RGCGCY 2 cut(s) 413, 594
HaeIII GGCC 3 cut(s) 272, 330, 341
HapII CCGG 4 cut(s) 50, 65, 342, 371
HgaI GACGC 1 cut(s) 77
HhaI GCGC 4 cut(s) 249, 268, 412, 593
Hin1I GRCGYC 2 cut(s) 69, 591
Hin1II CATG 3 cut(s) 194, 210, 802
Hin6I GCGC 4 cut(s) 247, 266, 410, 591
HinP1I GCGC 4 cut(s) 247, 266, 410, 591
HindIII AAGCTT 1 cut(s) 536
HinfI GANTC 3 cut(s) 426, 751, 869
HpaII CCGG 4 cut(s) 50, 65, 342, 371
HphI GGTGA 1 cut(s) 568
Hpy166II GTNNAC 3 cut(s) 56, 466, 834
Hpy188I TCNGA 6 cut(s) 298, 520, 756, 820, 877, 886
Hpy188III TCNNGA 2 cut(s) 395, 506
Hpy8I GTNNAC 3 cut(s) 56, 466, 834
Hpy99I CGWCG 1 cut(s) 815
HpyAV CCTTC 3 cut(s) 25, 55, 547
HpyCH4III ACNGT 4 cut(s) 635, 716, 775, 845
HpyCH4V TGCA 8 cut(s) 30, 131, 169, 244, 490, 631, 703, 834
HpyF3I CTNAG 2 cut(s) 151, 232
Hsp92I GRCGYC 2 cut(s) 69, 591
Hsp92II CATG 3 cut(s) 194, 210, 802
HspAI GCGC 4 cut(s) 247, 266, 410, 591
KasI GGCGCC 1 cut(s) 590
Ksp22I TGATCA 1 cut(s) 886
Kzo9I GATC 3 cut(s) 355, 820, 886
LguI GCTCTTC 1 cut(s) 191
LmnI GCTCC 6 cut(s) 79, 215, 623, 744, 821, 983
Lsp1109I GCAGC 6 cut(s) 153, 256, 354, 597, 602, 923
LweI GCATC 2 cut(s) 217, 912
MaeI CTAG 1 cut(s) 991
MaeIII GTNAC 4 cut(s) 236, 775, 845, 939
MalI GATC 3 cut(s) 357, 822, 888
MbiI CCGCTC 2 cut(s) 74, 305
MboI GATC 3 cut(s) 355, 820, 886
MboII GAAGA 7 cut(s) 10, 13, 28, 178, 571, 667, 864
MhlI GDGCHC 4 cut(s) 164, 186, 332, 836
MluCI AATT 3 cut(s) 667, 677, 879
Mly113I GGCGCC 1 cut(s) 591
MmeI TCCRAC 2 cut(s) 171, 546
MseI TTAA 1 cut(s) 648
MslI CAYNNNNRTG 1 cut(s) 698
MspA1I CMGCKG 3 cut(s) 166, 588, 721
MspI CCGG 4 cut(s) 50, 65, 342, 371
MspR9I CCNGG 3 cut(s) 50, 51, 65
Mva1269I GAATGC 3 cut(s) 30, 131, 858
NarI GGCGCC 1 cut(s) 591
NciI CCSGG 3 cut(s) 50, 51, 65
NcoI CCATGG 1 cut(s) 190
NdeII GATC 3 cut(s) 355, 820, 886
NlaIII CATG 3 cut(s) 194, 210, 802
NlaIV GGNNCC 5 cut(s) 161, 330, 592, 740, 817
NmeAIII GCCGAG 1 cut(s) 288
NmuCI GTSAC 3 cut(s) 236, 845, 939
NspI RCATGY 1 cut(s) 210
PaeI GCATGC 1 cut(s) 210
PciSI GCTCTTC 1 cut(s) 191
PcsI WCGNNNNNNNCGW 1 cut(s) 915
PctI GAATGC 3 cut(s) 30, 131, 858
PfeI GAWTC 3 cut(s) 426, 751, 869
PkrI GCNGC 8 cut(s) 73, 168, 246, 369, 587, 590, 617, 913
PluTI GGCGCC 1 cut(s) 594
Psp124BI GAGCTC 1 cut(s) 186
PspFI CCCAGC 1 cut(s) 331
PspN4I GGNNCC 5 cut(s) 161, 330, 592, 740, 817
PspOMI GGGCCC 1 cut(s) 328
PspPI GGNCC 3 cut(s) 328, 329, 690
PvuII CAGCTG 1 cut(s) 721
RsaI GTAC 1 cut(s) 467
RsaNI GTAC 1 cut(s) 466
RseI CAYNNNNRTG 1 cut(s) 698
SacI GAGCTC 1 cut(s) 186
SapI GCTCTTC 1 cut(s) 191
SaqAI TTAA 1 cut(s) 648
SatI GCNGC 8 cut(s) 72, 167, 245, 368, 586, 589, 616, 912
Sau3AI GATC 3 cut(s) 355, 820, 886
Sau96I GGNCC 3 cut(s) 328, 329, 690
ScrFI CCNGG 3 cut(s) 50, 51, 65
SduI GDGCHC 4 cut(s) 164, 186, 332, 836
SfaNI GCATC 2 cut(s) 217, 912
SfoI GGCGCC 1 cut(s) 592
SinI GGWCC 1 cut(s) 690
SmaI CCCGGG 1 cut(s) 51
SmiMI CAYNNNNRTG 1 cut(s) 698
SmlI CTYRAG 2 cut(s) 496, 580
SmoI CTYRAG 2 cut(s) 496, 580
SphI GCATGC 1 cut(s) 210
Sse9I AATT 3 cut(s) 667, 677, 879
SsiI CCGC 9 cut(s) 72, 117, 164, 204, 213, 303, 359, 477, 588
SspDI GGCGCC 1 cut(s) 590
SspMI CTAG 1 cut(s) 991
SstI GAGCTC 1 cut(s) 186
StyD4I CCNGG 3 cut(s) 48, 49, 63
StyI CCWWGG 1 cut(s) 190
TaaI ACNGT 4 cut(s) 635, 716, 775, 845
TaqI TCGA 3 cut(s) 429, 790, 867
TaqII GACCGA 1 cut(s) 816
TasI AATT 3 cut(s) 667, 677, 879
TatI WGTACW 1 cut(s) 465
TauI GCSGC 2 cut(s) 74, 591
TfiI GAWTC 3 cut(s) 426, 751, 869
Tru1I TTAA 1 cut(s) 648
Tru9I TTAA 1 cut(s) 648
TscAI CASTG 3 cut(s) 342, 369, 850
TseFI GTSAC 3 cut(s) 236, 845, 939
TseI GCWGC 6 cut(s) 166, 244, 367, 585, 615, 911
Tsp45I GTSAC 3 cut(s) 236, 845, 939
TspGWI ACGGA 4 cut(s) 395, 772, 864, 907
TspMI CCCGGG 1 cut(s) 49
TspRI CASTG 3 cut(s) 342, 369, 850
VneI GTGCAC 1 cut(s) 832
VpaK11BI GGWCC 1 cut(s) 690
XapI RAATTY 1 cut(s) 879
XceI RCATGY 1 cut(s) 210
XmaI CCCGGG 1 cut(s) 49
XmiI GTMKAC 1 cut(s) 55
XspI CTAG 1 cut(s) 991
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.