MD14G1234700.v1.1

GPI-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0012959
Physical Location & Seq
Reverse (-)
31467940 .. 31470193
2254 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1234700.v1.1.491

Sequence Viewer

Length: 1002 bp
ATGTTCTTCTCCTTCTTCTTCTTCCTCTGCATTCTCTTCCCTGCCTTCCCGGGTCTACCCAACCCGGGTGCGGCTCCCACCCAGTCATTCCTCCCAAACCGGACTCCCTCCCCACCCACTACAATCCCTGCATTCCCAGAACAATCCGACTTAGTCGGGTGCCCGCTGCACCTCCCCAATGAGCTCTTCCATGGCATAAAGTCCGCATGCTCCGCCACCAAAGGCGATGCCTCAGGTCACTTGCAGCGCAGCAGGTGCTGTCCTGCGCTGGCCTCGTGGCTCTACTCTGCCTACTCTGACACCGCTCTCGGCAGAGTAGGCAGGGTGGGCTCAGCAGTGGCCGGTCAGTCTTATGATTTGCCACTGCTGCCGGACGACACGGAAACTTGTGTTCAGGACTTGGGAAAGGCGCTGAAAAACAGAGGTATCGAGCTAATCAAGCCGAATGAGACCTGTGATGTTGTGTACTGCTATTGCGGGATAAGATTGCACCCCTTGAGCTGTCCCGAGGCGTTTTCGGAGAACGAAAGAGGGAAGCTTGTTGGGGATGAAAAGGTGAAGAAGTTGGAGAGAGATTGCTTGAGCAGCGGCGCCAATGGCAATTGGTTTCCTGGTCTTGGTGGCTGCTCCAAATGCTTGCACAGTCTCTACAATCTTAACAAGAAGAAAACTTCCAATTCAAGCAAATTAGGGGACAGGACCACCAAAATGCACAACAAAGATTGTCAGCTGATGGGTCTCACTTGGCTCCTTGCCAAGAATCGGACGGCTTACATCCACACAGTTACATCCGTGTTTCGGGCTATCATGTTAAGCACCGACGGCTCCGATCCTCGGTCGTGCACGCTCAACAGTGACGGAATGCCTCTCGCTGTCGATTCTTCTGAAATTTCTGATCAGTCCTCGTCCAGCATCCTCGCAGCTTCCGTCTCGTTCTACATTGTAATGTCACTTTCTTTGTTTTTTATGTTGTCGCTTGCCCTATGCTCCATCCAAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

334

Amino Acids

36.06

Weight (kDa)

7.85

Isoelectric Point (pI)

47.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPARK PF19160 53 - 242 1.4e-08 SPARK
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012959)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18050
fragaria_vesca FvH4_5g17140 FvH4_5g17140
malus_domestica MD06G1230000.v1.1 MD14G1234700.v1.1
prunus_persica Prupe.5G231000_v2.0.a1
pyrus_communis pycom06g20120 pycom14g19570
rosa_chinensis RchiOBHm_Chr7g0178651
rosa_laevigata RLG00000005426
rosa_multiflora Rmu_sc0002320.1_g000008
rosa_roxburghii Rroxscaffold_3G00274740
rosa_rugosa Rorug06G0418700
rosa_samantha Rh7AG017500 Rh7BG017100 Rh7CG017700 Rh7DG017700
rosa_wichuraiana Rw7G001420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 243
Acc36I ACCTGC 1 cut(s) 243
AccB1I GGYRCC 2 cut(s) 159, 590
AccBSI CCGCTC 1 cut(s) 305
AccI GTMKAC 1 cut(s) 55
AciI CCGC 7 cut(s) 71, 164, 204, 213, 303, 477, 588
AclWI GGATC 1 cut(s) 824
AcoI YGGCCR 1 cut(s) 339
AcsI RAATTY 1 cut(s) 888
AcyI GRCGYC 1 cut(s) 591
AfaI GTAC 1 cut(s) 467
AfiI CCNNNNNNNGG 6 cut(s) 65, 70, 617, 762, 798, 834
AgsI TTSAA 1 cut(s) 681
AjnI CCWGG 1 cut(s) 610
AjuI GAANNNNNNNTTGG 2 cut(s) 170, 202
AluBI AGCT 6 cut(s) 184, 433, 501, 538, 730, 923
AluI AGCT 6 cut(s) 184, 433, 501, 538, 730, 923
Alw21I GWGCWC 2 cut(s) 186, 845
Alw26I GTCTC 4 cut(s) 443, 650, 743, 934
Alw44I GTGCAC 1 cut(s) 841
AlwI GGATC 1 cut(s) 824
AlwNI CAGNNNCTG 1 cut(s) 258
Ama87I CYCGRG 3 cut(s) 49, 64, 506
AoxI GGCC 2 cut(s) 270, 339
ApaLI GTGCAC 1 cut(s) 841
ApeKI GCWGC 7 cut(s) 166, 244, 249, 367, 585, 624, 920
ApoI RAATTY 1 cut(s) 888
AspLEI GCGC 4 cut(s) 249, 268, 412, 593
AspS9I GGNCC 1 cut(s) 699
AsuC2I CCSGG 4 cut(s) 50, 51, 65, 66
AsuHPI GGTGA 1 cut(s) 568
AvaI CYCGRG 3 cut(s) 49, 64, 506
AvaII GGWCC 1 cut(s) 699
AxyI CCTNAGG 1 cut(s) 232
BaeGI GKGCMC 2 cut(s) 164, 845
BaeI ACNNNNGTAYC 2 cut(s) 409, 442
BanI GGYRCC 2 cut(s) 159, 590
BanII GRGCYC 2 cut(s) 186, 332
BauI CACGAG 1 cut(s) 274
Bbv12I GWGCWC 2 cut(s) 186, 845
BbvI GCAGC 7 cut(s) 153, 256, 261, 354, 597, 611, 932
BccI CCATC 2 cut(s) 727, 998
BceAI ACGGC 2 cut(s) 783, 838
BciT130I CCWGG 1 cut(s) 612
BclI TGATCA 1 cut(s) 895
BcnI CCSGG 4 cut(s) 50, 51, 65, 66
BcoDI GTCTC 4 cut(s) 443, 650, 743, 934
BfaI CTAG 1 cut(s) 1000
BfoI RGCGCY 2 cut(s) 413, 594
BfuAI ACCTGC 1 cut(s) 243
BisI GCNGC 9 cut(s) 72, 167, 245, 250, 368, 586, 589, 625, 921
BlpI GCTNAGC 1 cut(s) 331
BlsI GCNGC 9 cut(s) 73, 168, 246, 251, 369, 587, 590, 626, 922
Bme1390I CCNGG 5 cut(s) 50, 51, 65, 66, 612
Bme18I GGWCC 1 cut(s) 699
BmeT110I CYCGRG 3 cut(s) 49, 64, 506
BmgT120I GGNCC 1 cut(s) 699
BmiI GGNNCC 5 cut(s) 75, 161, 592, 749, 826
BmrFI CCNGG 5 cut(s) 50, 51, 65, 66, 612
BmrI ACTGGG 1 cut(s) 76
BmsI GCATC 2 cut(s) 217, 921
BmuI ACTGGG 1 cut(s) 76
Bpu1102I GCTNAGC 1 cut(s) 331
BpuEI CTTGAG 2 cut(s) 517, 601
BpuMI CCSGG 4 cut(s) 50, 51, 65, 66
BsaHI GRCGYC 1 cut(s) 591
BsaI GGTCTC 2 cut(s) 443, 743
BsaJI CCNNGG 5 cut(s) 49, 64, 190, 507, 833
BsaWI WCCGGW 1 cut(s) 99
Bsc4I CCNNNNNNNGG 6 cut(s) 65, 70, 617, 762, 798, 834
Bse118I RCCGGY 1 cut(s) 341
Bse1I ACTGG 1 cut(s) 82
Bse21I CCTNAGG 1 cut(s) 232
BseBI CCWGG 1 cut(s) 612
BseDI CCNNGG 5 cut(s) 49, 64, 190, 507, 833
BseGI GGATG 5 cut(s) 553, 774, 788, 912, 990
BseLI CCNNNNNNNGG 6 cut(s) 65, 70, 617, 762, 798, 834
BseMII CTCAG 2 cut(s) 246, 345
BseNI ACTGG 1 cut(s) 82
BseSI GKGCMC 2 cut(s) 164, 845
BseXI GCAGC 7 cut(s) 153, 256, 261, 354, 597, 611, 932
BsgI GTGCAG 1 cut(s) 152
Bsh1285I CGRYCG 1 cut(s) 839
BshFI GGCC 2 cut(s) 272, 341
BshNI GGYRCC 2 cut(s) 159, 590
BsiEI CGRYCG 1 cut(s) 839
BsiHKAI GWGCWC 2 cut(s) 186, 845
BsiHKCI CYCGRG 3 cut(s) 49, 64, 506
BsiSI CCGG 5 cut(s) 50, 65, 100, 342, 371
BslFI GGGAC 2 cut(s) 489, 707
BslI CCNNNNNNNGG 6 cut(s) 65, 70, 617, 762, 798, 834
BsmAI GTCTC 4 cut(s) 443, 650, 743, 934
BsmBI CGTCTC 1 cut(s) 934
BsmFI GGGAC 2 cut(s) 489, 707
BsmI GAATGC 3 cut(s) 30, 131, 867
BsnI GGCC 2 cut(s) 272, 341
Bso31I GGTCTC 2 cut(s) 443, 743
BsoBI CYCGRG 3 cut(s) 49, 64, 506
Bsp1286I GDGCHC 4 cut(s) 164, 186, 332, 845
Bsp143I GATC 2 cut(s) 829, 895
Bsp1720I GCTNAGC 1 cut(s) 331
Bsp19I CCATGG 1 cut(s) 190
BspACI CCGC 7 cut(s) 71, 164, 204, 213, 303, 477, 588
BspANI GGCC 2 cut(s) 272, 341
BspCNI CTCAG 2 cut(s) 245, 344
BspLI GGNNCC 5 cut(s) 75, 161, 592, 749, 826
BspMI ACCTGC 1 cut(s) 243
BspPI GGATC 1 cut(s) 824
BspQI GCTCTTC 1 cut(s) 191
BspT107I GGYRCC 2 cut(s) 159, 590
BspTNI GGTCTC 2 cut(s) 443, 743
BsrBI CCGCTC 1 cut(s) 305
BsrFI RCCGGY 1 cut(s) 341
BsrI ACTGG 1 cut(s) 82
BssAI RCCGGY 1 cut(s) 341
BssECI CCNNGG 5 cut(s) 49, 64, 190, 507, 833
BssMI GATC 2 cut(s) 829, 895
BssNI GRCGYC 1 cut(s) 591
BssSI CACGAG 1 cut(s) 274
BssT1I CCWWGG 1 cut(s) 190
Bst2BI CACGAG 1 cut(s) 274
Bst2UI CCWGG 1 cut(s) 612
Bst4CI ACNGT 3 cut(s) 644, 784, 854
Bst6I CTCTTC 2 cut(s) 41, 191
BstACI GRCGYC 1 cut(s) 591
BstAPI GCANNNNNTGC 1 cut(s) 255
BstC8I GCNNGC 6 cut(s) 164, 208, 270, 638, 845, 978
BstDEI CTNAG 3 cut(s) 151, 232, 331
BstDSI CCRYGG 1 cut(s) 190
BstF5I GGATG 5 cut(s) 553, 774, 788, 912, 990
BstH2I RGCGCY 2 cut(s) 413, 594
BstHHI GCGC 4 cut(s) 249, 268, 412, 593
BstKTI GATC 2 cut(s) 832, 898
BstMAI GTCTC 4 cut(s) 443, 650, 743, 934
BstMBI GATC 2 cut(s) 829, 895
BstMCI CGRYCG 1 cut(s) 839
BstNI CCWGG 1 cut(s) 612
BstNSI RCATGY 1 cut(s) 210
BstSCI CCNGG 5 cut(s) 48, 49, 63, 64, 610
BstSLI GKGCMC 2 cut(s) 164, 845
BstV1I GCAGC 7 cut(s) 153, 256, 261, 354, 597, 611, 932
Bsu36I CCTNAGG 1 cut(s) 232
BsuRI GGCC 2 cut(s) 272, 341
BtgI CCRYGG 1 cut(s) 190
BtgZI GCGATG 1 cut(s) 240
BtsCI GGATG 5 cut(s) 553, 774, 788, 912, 990
BtsI GCAGTG 2 cut(s) 342, 362
BtsIMutI CAGTG 3 cut(s) 342, 362, 859
BveI ACCTGC 1 cut(s) 243
Cac8I GCNNGC 6 cut(s) 164, 208, 270, 638, 845, 978
CaiI CAGNNNCTG 1 cut(s) 258
CfoI GCGC 4 cut(s) 249, 268, 412, 593
Cfr10I RCCGGY 1 cut(s) 341
Cfr13I GGNCC 1 cut(s) 699
Cfr9I CCCGGG 2 cut(s) 49, 64
Csp6I GTAC 1 cut(s) 466
CviAII CATG 3 cut(s) 191, 207, 808
CviQI GTAC 1 cut(s) 466
DdeI CTNAG 3 cut(s) 151, 232, 331
DinI GGCGCC 1 cut(s) 592
DpnI GATC 2 cut(s) 831, 897
DpnII GATC 2 cut(s) 829, 895
EaeI YGGCCR 1 cut(s) 339
Eam1104I CTCTTC 2 cut(s) 41, 191
EarI CTCTTC 2 cut(s) 41, 191
EciI GGCGGA 1 cut(s) 202
Ecl136II GAGCTC 1 cut(s) 184
Eco130I CCWWGG 1 cut(s) 190
Eco24I GRGCYC 2 cut(s) 186, 332
Eco31I GGTCTC 2 cut(s) 443, 743
Eco47I GGWCC 1 cut(s) 699
Eco53kI GAGCTC 1 cut(s) 184
Eco81I CCTNAGG 1 cut(s) 232
Eco88I CYCGRG 3 cut(s) 49, 64, 506
EcoICRI GAGCTC 1 cut(s) 184
EcoRII CCWGG 1 cut(s) 610
EcoT14I CCWWGG 1 cut(s) 190
EcoT38I GRGCYC 2 cut(s) 186, 332
EgeI GGCGCC 1 cut(s) 592
EheI GGCGCC 1 cut(s) 592
ErhI CCWWGG 1 cut(s) 190
Esp3I CGTCTC 1 cut(s) 934
FaeI CATG 3 cut(s) 194, 210, 811
FaiI YATR 7 cut(s) 192, 197, 208, 354, 809, 968, 985
FaqI GGGAC 2 cut(s) 489, 707
FatI CATG 3 cut(s) 190, 206, 807
FauI CCCGC 2 cut(s) 171, 470
FbaI TGATCA 1 cut(s) 895
FblI GTMKAC 1 cut(s) 55
Fnu4HI GCNGC 9 cut(s) 72, 167, 245, 250, 368, 586, 589, 625, 921
FokI GGATG 5 cut(s) 560, 761, 775, 899, 977
FriOI GRGCYC 2 cut(s) 186, 332
Fsp4HI GCNGC 9 cut(s) 72, 167, 245, 250, 368, 586, 589, 625, 921
FspBI CTAG 1 cut(s) 1000
GlaI GCGC 4 cut(s) 248, 267, 411, 592
GluI GCNGC 9 cut(s) 72, 167, 245, 250, 368, 586, 589, 625, 921
HaeII RGCGCY 2 cut(s) 413, 594
HaeIII GGCC 2 cut(s) 272, 341
HapII CCGG 5 cut(s) 50, 65, 100, 342, 371
HhaI GCGC 4 cut(s) 249, 268, 412, 593
Hin1I GRCGYC 1 cut(s) 591
Hin1II CATG 3 cut(s) 194, 210, 811
Hin6I GCGC 4 cut(s) 247, 266, 410, 591
HinP1I GCGC 4 cut(s) 247, 266, 410, 591
HindIII AAGCTT 1 cut(s) 536
HinfI GANTC 3 cut(s) 103, 760, 878
HpaII CCGG 5 cut(s) 50, 65, 100, 342, 371
HphI GGTGA 1 cut(s) 568
Hpy166II GTNNAC 3 cut(s) 56, 466, 843
Hpy188I TCNGA 7 cut(s) 148, 298, 520, 765, 829, 886, 895
Hpy188III TCNNGA 2 cut(s) 395, 506
Hpy8I GTNNAC 3 cut(s) 56, 466, 843
Hpy99I CGWCG 1 cut(s) 824
HpyAV CCTTC 2 cut(s) 22, 55
HpyCH4III ACNGT 3 cut(s) 644, 784, 854
HpyCH4V TGCA 8 cut(s) 30, 131, 169, 244, 490, 640, 712, 843
HpyF3I CTNAG 3 cut(s) 151, 232, 331
Hsp92I GRCGYC 1 cut(s) 591
Hsp92II CATG 3 cut(s) 194, 210, 811
HspAI GCGC 4 cut(s) 247, 266, 410, 591
KasI GGCGCC 1 cut(s) 590
Ksp22I TGATCA 1 cut(s) 895
Kzo9I GATC 2 cut(s) 829, 895
LguI GCTCTTC 1 cut(s) 191
LmnI GCTCC 6 cut(s) 79, 215, 632, 753, 830, 992
Lsp1109I GCAGC 7 cut(s) 153, 256, 261, 354, 597, 611, 932
LweI GCATC 2 cut(s) 217, 921
MaeI CTAG 1 cut(s) 1000
MaeIII GTNAC 4 cut(s) 236, 784, 854, 948
MalI GATC 2 cut(s) 831, 897
MbiI CCGCTC 1 cut(s) 305
MboI GATC 2 cut(s) 829, 895
MboII GAAGA 8 cut(s) 7, 10, 13, 28, 178, 571, 676, 873
MfeI CAATTG 1 cut(s) 601
MhlI GDGCHC 4 cut(s) 164, 186, 332, 845
MluCI AATT 4 cut(s) 601, 676, 686, 888
Mly113I GGCGCC 1 cut(s) 591
MlyI GAGTC 1 cut(s) 97
MmeI TCCRAC 2 cut(s) 171, 546
MseI TTAA 2 cut(s) 657, 812
MslI CAYNNNNRTG 2 cut(s) 707, 944
MspA1I CMGCKG 3 cut(s) 166, 588, 730
MspI CCGG 5 cut(s) 50, 65, 100, 342, 371
MspR9I CCNGG 5 cut(s) 50, 51, 65, 66, 612
MunI CAATTG 1 cut(s) 601
Mva1269I GAATGC 3 cut(s) 30, 131, 867
MvaI CCWGG 1 cut(s) 612
NarI GGCGCC 1 cut(s) 591
NciI CCSGG 4 cut(s) 50, 51, 65, 66
NcoI CCATGG 1 cut(s) 190
NdeII GATC 2 cut(s) 829, 895
NlaIII CATG 3 cut(s) 194, 210, 811
NlaIV GGNNCC 5 cut(s) 75, 161, 592, 749, 826
NmeAIII GCCGAG 1 cut(s) 288
NmuCI GTSAC 3 cut(s) 236, 854, 948
NspI RCATGY 1 cut(s) 210
PaeI GCATGC 1 cut(s) 210
PaqCI CACCTGC 1 cut(s) 243
PciSI GCTCTTC 1 cut(s) 191
PcsI WCGNNNNNNNCGW 1 cut(s) 924
PctI GAATGC 3 cut(s) 30, 131, 867
PfeI GAWTC 2 cut(s) 760, 878
PflFI GACNNNGTC 1 cut(s) 152
PkrI GCNGC 9 cut(s) 73, 168, 246, 251, 369, 587, 590, 626, 922
PleI GAGTC 1 cut(s) 97
PluTI GGCGCC 1 cut(s) 594
PpsI GAGTC 1 cut(s) 97
Psp124BI GAGCTC 1 cut(s) 186
Psp6I CCWGG 1 cut(s) 610
PspGI CCWGG 1 cut(s) 610
PspN4I GGNNCC 5 cut(s) 75, 161, 592, 749, 826
PspPI GGNCC 1 cut(s) 699
PstNI CAGNNNCTG 1 cut(s) 258
PsyI GACNNNGTC 1 cut(s) 152
PvuII CAGCTG 1 cut(s) 730
RsaI GTAC 1 cut(s) 467
RsaNI GTAC 1 cut(s) 466
RseI CAYNNNNRTG 2 cut(s) 707, 944
SacI GAGCTC 1 cut(s) 186
SapI GCTCTTC 1 cut(s) 191
SaqAI TTAA 2 cut(s) 657, 812
SatI GCNGC 9 cut(s) 72, 167, 245, 250, 368, 586, 589, 625, 921
Sau3AI GATC 2 cut(s) 829, 895
Sau96I GGNCC 1 cut(s) 699
SchI GAGTC 1 cut(s) 97
ScrFI CCNGG 5 cut(s) 50, 51, 65, 66, 612
SduI GDGCHC 4 cut(s) 164, 186, 332, 845
SfaNI GCATC 2 cut(s) 217, 921
SfoI GGCGCC 1 cut(s) 592
SinI GGWCC 1 cut(s) 699
SmaI CCCGGG 2 cut(s) 51, 66
SmiMI CAYNNNNRTG 2 cut(s) 707, 944
SmlI CTYRAG 2 cut(s) 496, 580
SmoI CTYRAG 2 cut(s) 496, 580
SphI GCATGC 1 cut(s) 210
Sse9I AATT 4 cut(s) 601, 676, 686, 888
SsiI CCGC 7 cut(s) 71, 164, 204, 213, 303, 477, 588
SspDI GGCGCC 1 cut(s) 590
SspMI CTAG 1 cut(s) 1000
SstI GAGCTC 1 cut(s) 186
StyD4I CCNGG 5 cut(s) 48, 49, 63, 64, 610
StyI CCWWGG 1 cut(s) 190
TaaI ACNGT 3 cut(s) 644, 784, 854
TaqI TCGA 2 cut(s) 429, 876
TaqII GACCGA 1 cut(s) 825
TasI AATT 4 cut(s) 601, 676, 686, 888
TatI WGTACW 1 cut(s) 465
TauI GCSGC 2 cut(s) 74, 591
TfiI GAWTC 2 cut(s) 760, 878
Tru1I TTAA 2 cut(s) 657, 812
Tru9I TTAA 2 cut(s) 657, 812
TscAI CASTG 3 cut(s) 342, 369, 859
TseFI GTSAC 3 cut(s) 236, 854, 948
TseI GCWGC 7 cut(s) 166, 244, 249, 367, 585, 624, 920
Tsp45I GTSAC 3 cut(s) 236, 854, 948
TspDTI ATGAA 1 cut(s) 564
TspGWI ACGGA 4 cut(s) 395, 781, 873, 916
TspMI CCCGGG 2 cut(s) 49, 64
TspRI CASTG 3 cut(s) 342, 369, 859
Tth111I GACNNNGTC 1 cut(s) 152
VneI GTGCAC 1 cut(s) 841
VpaK11BI GGWCC 1 cut(s) 699
XapI RAATTY 1 cut(s) 888
XceI RCATGY 1 cut(s) 210
XmaI CCCGGG 2 cut(s) 49, 64
XmiI GTMKAC 1 cut(s) 55
XspI CTAG 1 cut(s) 1000
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.