MD06G1230000.v1.1

GPI-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0012959
Physical Location & Seq
Reverse (-)
35971912 .. 35974366
2455 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1230000.v1.1.491

Sequence Viewer

Length: 993 bp
ATGTCCTTCACCATCTTCTTCCTTTACATTCTCTTCCCTGCCTTCCCGGGTCTACCCAACCCGGACCCGGCTTCCATCCAGTCATTCCACCCGGCCCGAACTCCCTCCCCATCCGCTACAATCCCTGCATTCCCAGAACAATCCGACTTAGCCGGGTGCCCGCTGGACCTCCCCAATGAGCTCTTCCACGGCATAAAGTCTGCCTGCTCCGCCACCAAAGGTGACTCCTCTGACCACCTACAGCGAAGCAGGTGCTGCCCTGCGCTCGCGGCGTGGCTCTACTCTGCCTACTCTGCCACCGCTCTCGGCAGAGTAGGCAGGGTGAGCCCAGCAGTGTCGGGTCAGCCTTATGATCTGCCACTGCTGCCAGACGACTCGGAAACTTGCGTCGAGGACTTGGGACAGGCGCTGAAAACAAGAGGAATTGAGCTAATCAAGCCGAATGAAACCTGTGATGTTGTGTATTGCTATTGCGGGATAAGATTGCACCCCTTGAGCTGTCCCGAGGCCTTTTGGGAGAACGAAAGGGGGAAGCTTGTTGGGGGTGCGAAGGTGAAGAAGTTGGAGAGAGATTGCTTGAGCGGCGGCGGCAATGGGTTTCCGGGTCTTGGCGGCTGCTCCAAATGCTTGCACAGTCTCTACAATCTTAACAAGAAGAAAGCTTCGGATTCAAGCAAATTAGAGGACAGGACCACCAAAATGCACAGCAAAGACTGTCAGTTGATGGGTCTCACTTGGCTCCTTGCCAAGAATCGGACGGCTTACATCCACACGGTGACATCTGTCTTCCGGGCTTTCATGGTAAGCGCCGACGGGTCTGATCCTCGGTCGTGCACGCTCAACAGCGATGGAATGCCTCTTGCCGTCGATTCTTCTCAAATTTCTGATCAGTCCTCATCCAACATCCTTGCAGCTTCCGTCTTTTTCTACCTTGTAATGTCACTTTCTTTGTTGTTTATGTTGCAGCTTACCCTATCCTCCATCCAAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

331

Amino Acids

35.68

Weight (kDa)

6.64

Isoelectric Point (pI)

55.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPARK PF19160 51 - 239 2.5e-08 SPARK
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012959)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18050
fragaria_vesca FvH4_5g17140 FvH4_5g17140
malus_domestica MD06G1230000.v1.1 MD14G1234700.v1.1
prunus_persica Prupe.5G231000_v2.0.a1
pyrus_communis pycom06g20120 pycom14g19570
rosa_chinensis RchiOBHm_Chr7g0178651
rosa_laevigata RLG00000005426
rosa_multiflora Rmu_sc0002320.1_g000008
rosa_roxburghii Rroxscaffold_3G00274740
rosa_rugosa Rorug06G0418700
rosa_samantha Rh7AG017500 Rh7BG017100 Rh7CG017700 Rh7DG017700
rosa_wichuraiana Rw7G001420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 240
Acc36I ACCTGC 1 cut(s) 240
AccB1I GGYRCC 1 cut(s) 156
AccBSI CCGCTC 2 cut(s) 302, 582
AccI GTMKAC 1 cut(s) 52
AccII CGCG 1 cut(s) 269
AclWI GGATC 1 cut(s) 815
AcsI RAATTY 1 cut(s) 879
AdeI CACNNNGTG 1 cut(s) 775
AfiI CCNNNNNNNGG 3 cut(s) 67, 608, 753
AgsI TTSAA 1 cut(s) 672
AjuI GAANNNNNNNTTGG 2 cut(s) 167, 199
AluBI AGCT 7 cut(s) 181, 430, 498, 535, 662, 914, 967
AluI AGCT 7 cut(s) 181, 430, 498, 535, 662, 914, 967
Alw21I GWGCWC 2 cut(s) 183, 836
Alw26I GTCTC 2 cut(s) 641, 734
Alw44I GTGCAC 1 cut(s) 832
AlwI GGATC 1 cut(s) 815
AlwNI CAGNNNCTG 2 cut(s) 255, 409
Ama87I CYCGRG 2 cut(s) 46, 503
AoxI GGCC 2 cut(s) 93, 507
ApaLI GTGCAC 1 cut(s) 832
ApeKI GCWGC 5 cut(s) 255, 364, 615, 911, 964
ApoI RAATTY 1 cut(s) 879
AspLEI GCGC 3 cut(s) 265, 409, 809
AspS9I GGNCC 4 cut(s) 64, 94, 166, 690
AsuC2I CCSGG 8 cut(s) 47, 48, 62, 68, 92, 154, 603, 791
AsuHPI GGTGA 4 cut(s) 233, 334, 565, 787
AvaI CYCGRG 2 cut(s) 46, 503
AvaII GGWCC 3 cut(s) 64, 166, 690
BaeGI GKGCMC 2 cut(s) 161, 836
BanI GGYRCC 1 cut(s) 156
BanII GRGCYC 2 cut(s) 183, 329
BbsI GAAGAC 1 cut(s) 778
Bbv12I GWGCWC 2 cut(s) 183, 836
BbvI GCAGC 5 cut(s) 242, 351, 602, 923, 976
BccI CCATC 6 cut(s) 20, 83, 118, 718, 842, 989
BceAI ACGGC 3 cut(s) 205, 774, 848
BcgI CGANNNNNNTGC 2 cut(s) 234, 268
BclI TGATCA 1 cut(s) 886
BcnI CCSGG 8 cut(s) 47, 48, 62, 68, 92, 154, 603, 791
BcoDI GTCTC 2 cut(s) 641, 734
BfaI CTAG 1 cut(s) 991
BfmI CTRYAG 1 cut(s) 239
BfoI RGCGCY 2 cut(s) 410, 810
BfuAI ACCTGC 1 cut(s) 240
Bme1390I CCNGG 8 cut(s) 47, 48, 62, 68, 92, 154, 603, 791
Bme18I GGWCC 3 cut(s) 64, 166, 690
BmeT110I CYCGRG 2 cut(s) 46, 503
BmgT120I GGNCC 4 cut(s) 64, 94, 166, 690
BmiI GGNNCC 3 cut(s) 66, 158, 740
BmrFI CCNGG 8 cut(s) 47, 48, 62, 68, 92, 154, 603, 791
BoxI GACNNNNGTC 1 cut(s) 782
BpiI GAAGAC 1 cut(s) 778
BpuEI CTTGAG 2 cut(s) 514, 598
BpuMI CCSGG 8 cut(s) 47, 48, 62, 68, 92, 154, 603, 791
BsaI GGTCTC 1 cut(s) 734
BsaJI CCNNGG 4 cut(s) 46, 187, 504, 824
Bsc4I CCNNNNNNNGG 3 cut(s) 67, 608, 753
Bse1I ACTGG 1 cut(s) 79
Bse3DI GCAATG 1 cut(s) 598
BseDI CCNNGG 4 cut(s) 46, 187, 504, 824
BseGI GGATG 6 cut(s) 75, 110, 765, 896, 903, 981
BseLI CCNNNNNNNGG 3 cut(s) 67, 608, 753
BseMI GCAATG 1 cut(s) 598
BseNI ACTGG 1 cut(s) 79
BseRI GAGGAG 1 cut(s) 217
BseSI GKGCMC 2 cut(s) 161, 836
BseXI GCAGC 5 cut(s) 242, 351, 602, 923, 976
BseYI CCCAGC 1 cut(s) 328
Bsh1236I CGCG 1 cut(s) 269
Bsh1285I CGRYCG 1 cut(s) 830
BshFI GGCC 2 cut(s) 95, 509
BshNI GGYRCC 1 cut(s) 156
BsiEI CGRYCG 1 cut(s) 830
BsiHKAI GWGCWC 2 cut(s) 183, 836
BsiHKCI CYCGRG 2 cut(s) 46, 503
BsiSI CCGG 7 cut(s) 47, 62, 68, 92, 153, 602, 790
BslFI GGGAC 2 cut(s) 414, 486
BslI CCNNNNNNNGG 3 cut(s) 67, 608, 753
BsmAI GTCTC 2 cut(s) 641, 734
BsmFI GGGAC 2 cut(s) 414, 486
BsmI GAATGC 2 cut(s) 128, 858
BsnI GGCC 2 cut(s) 95, 509
Bso31I GGTCTC 1 cut(s) 734
BsoBI CYCGRG 2 cut(s) 46, 503
Bsp1286I GDGCHC 4 cut(s) 161, 183, 329, 836
Bsp143I GATC 3 cut(s) 352, 820, 886
BspANI GGCC 2 cut(s) 95, 509
BspFNI CGCG 1 cut(s) 269
BspLI GGNNCC 3 cut(s) 66, 158, 740
BspMI ACCTGC 1 cut(s) 240
BspPI GGATC 1 cut(s) 815
BspQI GCTCTTC 1 cut(s) 188
BspT107I GGYRCC 1 cut(s) 156
BspTNI GGTCTC 1 cut(s) 734
BsrBI CCGCTC 2 cut(s) 302, 582
BsrDI GCAATG 1 cut(s) 598
BsrI ACTGG 1 cut(s) 79
BssECI CCNNGG 4 cut(s) 46, 187, 504, 824
BssMI GATC 3 cut(s) 352, 820, 886
Bst4CI ACNGT 3 cut(s) 635, 716, 775
Bst6I CTCTTC 2 cut(s) 38, 188
BstAPI GCANNNNNTGC 1 cut(s) 255
BstC8I GCNNGC 5 cut(s) 161, 205, 267, 629, 836
BstDEI CTNAG 1 cut(s) 148
BstDSI CCRYGG 1 cut(s) 187
BstF5I GGATG 6 cut(s) 75, 110, 765, 896, 903, 981
BstFNI CGCG 1 cut(s) 269
BstH2I RGCGCY 2 cut(s) 410, 810
BstHHI GCGC 3 cut(s) 265, 409, 809
BstKTI GATC 3 cut(s) 355, 823, 889
BstMAI GTCTC 2 cut(s) 641, 734
BstMBI GATC 3 cut(s) 352, 820, 886
BstMCI CGRYCG 1 cut(s) 830
BstPAI GACNNNNGTC 1 cut(s) 782
BstSCI CCNGG 8 cut(s) 45, 46, 60, 66, 90, 152, 601, 789
BstSFI CTRYAG 1 cut(s) 239
BstSLI GKGCMC 2 cut(s) 161, 836
BstUI CGCG 1 cut(s) 269
BstV1I GCAGC 5 cut(s) 242, 351, 602, 923, 976
BstV2I GAAGAC 1 cut(s) 778
BsuRI GGCC 2 cut(s) 95, 509
BtgI CCRYGG 1 cut(s) 187
BtgZI GCGATG 1 cut(s) 861
BtsCI GGATG 6 cut(s) 75, 110, 765, 896, 903, 981
BtsI GCAGTG 2 cut(s) 339, 359
BtsIMutI CAGTG 2 cut(s) 339, 359
BveI ACCTGC 1 cut(s) 240
Cac8I GCNNGC 5 cut(s) 161, 205, 267, 629, 836
CaiI CAGNNNCTG 2 cut(s) 255, 409
CfoI GCGC 3 cut(s) 265, 409, 809
Cfr13I GGNCC 4 cut(s) 64, 94, 166, 690
Cfr9I CCCGGG 1 cut(s) 46
CseI GACGC 1 cut(s) 376
CviAII CATG 1 cut(s) 799
DdeI CTNAG 1 cut(s) 148
DpnI GATC 3 cut(s) 354, 822, 888
DpnII GATC 3 cut(s) 352, 820, 886
DraIII CACNNNGTG 1 cut(s) 775
Eam1104I CTCTTC 2 cut(s) 38, 188
EarI CTCTTC 2 cut(s) 38, 188
EciI GGCGGA 1 cut(s) 199
Ecl136II GAGCTC 1 cut(s) 181
Eco147I AGGCCT 1 cut(s) 509
Eco24I GRGCYC 2 cut(s) 183, 329
Eco31I GGTCTC 1 cut(s) 734
Eco47I GGWCC 3 cut(s) 64, 166, 690
Eco53kI GAGCTC 1 cut(s) 181
Eco88I CYCGRG 2 cut(s) 46, 503
EcoICRI GAGCTC 1 cut(s) 181
EcoT38I GRGCYC 2 cut(s) 183, 329
FaeI CATG 1 cut(s) 802
FaiI YATR 4 cut(s) 194, 351, 800, 959
FaqI GGGAC 2 cut(s) 414, 486
FatI CATG 1 cut(s) 798
FauI CCCGC 2 cut(s) 168, 467
FbaI TGATCA 1 cut(s) 886
FblI GTMKAC 1 cut(s) 52
FokI GGATG 6 cut(s) 62, 97, 752, 883, 890, 968
FriOI GRGCYC 2 cut(s) 183, 329
FspBI CTAG 1 cut(s) 991
GlaI GCGC 3 cut(s) 264, 408, 808
GsaI CCCAGC 1 cut(s) 332
HaeII RGCGCY 2 cut(s) 410, 810
HaeIII GGCC 2 cut(s) 95, 509
HapII CCGG 7 cut(s) 47, 62, 68, 92, 153, 602, 790
HgaI GACGC 1 cut(s) 376
HhaI GCGC 3 cut(s) 265, 409, 809
Hin1II CATG 1 cut(s) 802
Hin6I GCGC 3 cut(s) 263, 407, 807
HinP1I GCGC 3 cut(s) 263, 407, 807
HindIII AAGCTT 2 cut(s) 533, 660
HinfI GANTC 5 cut(s) 224, 374, 668, 751, 869
HpaII CCGG 7 cut(s) 47, 62, 68, 92, 153, 602, 790
HphI GGTGA 4 cut(s) 233, 334, 565, 787
Hpy166II GTNNAC 2 cut(s) 53, 834
Hpy188I TCNGA 7 cut(s) 145, 232, 379, 667, 756, 820, 886
Hpy188III TCNNGA 1 cut(s) 503
Hpy8I GTNNAC 2 cut(s) 53, 834
Hpy99I CGWCG 3 cut(s) 392, 815, 869
HpyAV CCTTC 3 cut(s) 16, 52, 544
HpyCH4III ACNGT 3 cut(s) 635, 716, 775
HpyCH4V TGCA 7 cut(s) 128, 487, 631, 703, 834, 911, 964
HpyF3I CTNAG 1 cut(s) 148
Hsp92II CATG 1 cut(s) 802
HspAI GCGC 3 cut(s) 263, 407, 807
Ksp22I TGATCA 1 cut(s) 886
Kzo9I GATC 3 cut(s) 352, 820, 886
LguI GCTCTTC 1 cut(s) 188
LmnI GCTCC 3 cut(s) 212, 623, 744
Lsp1109I GCAGC 5 cut(s) 242, 351, 602, 923, 976
MaeI CTAG 1 cut(s) 991
MaeIII GTNAC 3 cut(s) 221, 775, 939
MalI GATC 3 cut(s) 354, 822, 888
MbiI CCGCTC 2 cut(s) 302, 582
MboI GATC 3 cut(s) 352, 820, 886
MboII GAAGA 8 cut(s) 7, 10, 25, 175, 568, 667, 778, 864
MhlI GDGCHC 4 cut(s) 161, 183, 329, 836
MluCI AATT 3 cut(s) 423, 677, 879
MlyI GAGTC 2 cut(s) 218, 368
MmeI TCCRAC 3 cut(s) 168, 543, 924
MseI TTAA 1 cut(s) 648
MslI CAYNNNNRTG 1 cut(s) 698
MspA1I CMGCKG 1 cut(s) 163
MspI CCGG 7 cut(s) 47, 62, 68, 92, 153, 602, 790
MspR9I CCNGG 8 cut(s) 47, 48, 62, 68, 92, 154, 603, 791
Mva1269I GAATGC 2 cut(s) 128, 858
MvnI CGCG 1 cut(s) 269
NciI CCSGG 8 cut(s) 47, 48, 62, 68, 92, 154, 603, 791
NdeII GATC 3 cut(s) 352, 820, 886
NlaIII CATG 1 cut(s) 802
NlaIV GGNNCC 3 cut(s) 66, 158, 740
NmeAIII GCCGAG 1 cut(s) 285
NmuCI GTSAC 3 cut(s) 221, 775, 939
PaqCI CACCTGC 1 cut(s) 240
PceI AGGCCT 1 cut(s) 509
PciSI GCTCTTC 1 cut(s) 188
PctI GAATGC 2 cut(s) 128, 858
PfeI GAWTC 3 cut(s) 668, 751, 869
PleI GAGTC 2 cut(s) 218, 368
PpsI GAGTC 2 cut(s) 218, 368
PshAI GACNNNNGTC 1 cut(s) 782
Psp124BI GAGCTC 1 cut(s) 183
PspFI CCCAGC 1 cut(s) 328
PspN4I GGNNCC 3 cut(s) 66, 158, 740
PspPI GGNCC 4 cut(s) 64, 94, 166, 690
PstNI CAGNNNCTG 2 cut(s) 255, 409
RseI CAYNNNNRTG 1 cut(s) 698
SacI GAGCTC 1 cut(s) 183
SapI GCTCTTC 1 cut(s) 188
SaqAI TTAA 1 cut(s) 648
Sau3AI GATC 3 cut(s) 352, 820, 886
Sau96I GGNCC 4 cut(s) 64, 94, 166, 690
SchI GAGTC 2 cut(s) 218, 368
ScrFI CCNGG 8 cut(s) 47, 48, 62, 68, 92, 154, 603, 791
SduI GDGCHC 4 cut(s) 161, 183, 329, 836
SfcI CTRYAG 1 cut(s) 239
SinI GGWCC 3 cut(s) 64, 166, 690
SmaI CCCGGG 1 cut(s) 48
SmiMI CAYNNNNRTG 1 cut(s) 698
SmlI CTYRAG 2 cut(s) 493, 577
SmoI CTYRAG 2 cut(s) 493, 577
Sse9I AATT 3 cut(s) 423, 677, 879
SseBI AGGCCT 1 cut(s) 509
SspMI CTAG 1 cut(s) 991
SstI GAGCTC 1 cut(s) 183
StuI AGGCCT 1 cut(s) 509
StyD4I CCNGG 8 cut(s) 45, 46, 60, 66, 90, 152, 601, 789
TaaI ACNGT 3 cut(s) 635, 716, 775
TaqI TCGA 2 cut(s) 390, 867
TaqII GACCGA 1 cut(s) 816
TasI AATT 3 cut(s) 423, 677, 879
TauI GCSGC 5 cut(s) 272, 585, 588, 591, 615
TfiI GAWTC 3 cut(s) 668, 751, 869
Tru1I TTAA 1 cut(s) 648
Tru9I TTAA 1 cut(s) 648
TscAI CASTG 2 cut(s) 339, 366
TseFI GTSAC 3 cut(s) 221, 775, 939
TseI GCWGC 5 cut(s) 255, 364, 615, 911, 964
Tsp45I GTSAC 3 cut(s) 221, 775, 939
TspDTI ATGAA 2 cut(s) 459, 787
TspGWI ACGGA 1 cut(s) 907
TspMI CCCGGG 1 cut(s) 46
TspRI CASTG 2 cut(s) 339, 366
VneI GTGCAC 1 cut(s) 832
VpaK11BI GGWCC 3 cut(s) 64, 166, 690
XapI RAATTY 1 cut(s) 879
XmaI CCCGGG 1 cut(s) 46
XmiI GTMKAC 1 cut(s) 52
XspI CTAG 1 cut(s) 991
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.