RchiOBHm_Chr7g0178651

GPI-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0012959
Physical Location & Seq
Forward (+)
1248550 .. 1250611
2062 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ15921

Sequence Viewer

Length: 1017 bp
ATGTCCTCAAACCCACCACCACTCTTCACCTTCCTTTGCTTCCTCTTCACTTTGTTACCGTCCTCACTGGGTCTATCGGACCCGAAGCCGGCCTTGGTGCAGTCATTCCTTCCCAAATCTTCTCCCCCAGCTACAATCCCTGCATTCCCAGAGCAATCCAACATAGCCGGCTGCCCACTTCAGCTCCCTGATGAGCTCTTCCACGGCGTAAAGGAAGCCTGCTCCGCCAAAAAAGGCGACCCGACTGGCCATTTACAGCACAGCCGCTGCTGCCCAGTGCTGGCGGCGTGGCTATACTCGGCCTACTCCGCCACTGCACTGGGCAGAAGCGGCCAAGTAGGCCCTGCAGTGGCGGGTCACGGTAGTACTACTACTACTACCGCTTATGACCCGCCACTGCTTCCTGATGACTCAGAAACTTGTGTTCAGGACTTGGGAAAGGCACTGAACGCAAAAGGAATAGAGCTGATGAAGCCAAATGAGACCTGTGACATGGTCTATTGCTACTGTGGGATCAGATTGCACCCTTTGACCTGCCCTGAGGCATTTTCAGTGAACCAAAAAGGGAAACTTGTTGGGGATGAGAGTGTCAAGAAGTTGGAGAAGAATTGCTTGAGCAGCAGTACCAATGTCAATAAGTTCCCTGGTCTTGGTGGCTGCTCCAAATGCCTGAACAGTCTCTACCTTCTTAACAAGAAGAAGACTTCCAATTCAAGCAAAATGGAGGACAGGACCACCAAAATGCACAACAAAGACTGTCAGCTGATGGGTCTCACATGGCTTCTTGCCAAGAATCGGACGGCCTACATGCACACCGTTACATCCGTCTTCCGGGCTATCATGGTAAGCAATGACGGCTCTGATCCTCAGTCGTGCACTCTCAACAGTGACGGAATGCCTCTAGCCGTCGATTCGTCAGAAATCTCAGATCAATCCTCTTCCAGCATCCTTGCAGTTTCAATCACATTCTGCACAGTGTCACTTACTTTGTTGTATGTGCAGCTTACCCTGTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

338

Amino Acids

36.21

Weight (kDa)

7.43

Isoelectric Point (pI)

58.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012959)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18050
fragaria_vesca FvH4_5g17140 FvH4_5g17140
malus_domestica MD06G1230000.v1.1 MD14G1234700.v1.1
prunus_persica Prupe.5G231000_v2.0.a1
pyrus_communis pycom06g20120 pycom14g19570
rosa_chinensis RchiOBHm_Chr7g0178651
rosa_laevigata RLG00000005426
rosa_multiflora Rmu_sc0002320.1_g000008
rosa_roxburghii Rroxscaffold_3G00274740
rosa_rugosa Rorug06G0418700
rosa_samantha Rh7AG017500 Rh7BG017100 Rh7CG017700 Rh7DG017700
rosa_wichuraiana Rw7G001420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 542
AciI CCGC 8 cut(s) 225, 265, 284, 309, 330, 353, 381, 392
AclWI GGATC 2 cut(s) 521, 857
AcoI YGGCCR 2 cut(s) 247, 331
AcuI CTGAAG 1 cut(s) 164
AfaI GTAC 2 cut(s) 367, 625
AfiI CCNNNNNNNGG 6 cut(s) 88, 280, 349, 650, 795, 831
AgsI TTSAA 2 cut(s) 714, 960
AjnI CCWGG 1 cut(s) 643
AjuI GAANNNNNNNTTGG 2 cut(s) 77, 109
AluBI AGCT 6 cut(s) 131, 184, 196, 466, 763, 1003
AluI AGCT 6 cut(s) 131, 184, 196, 466, 763, 1003
Alw21I GWGCWC 2 cut(s) 198, 878
Alw26I GTCTC 3 cut(s) 476, 683, 776
Alw44I GTGCAC 1 cut(s) 874
AlwI GGATC 2 cut(s) 521, 857
AlwNI CAGNNNCTG 1 cut(s) 267
AoxI GGCC 6 cut(s) 90, 247, 300, 331, 340, 801
ApaLI GTGCAC 1 cut(s) 874
ApeKI GCWGC 6 cut(s) 171, 267, 270, 618, 657, 1000
AspS9I GGNCC 3 cut(s) 79, 341, 732
AsuC2I CCSGG 1 cut(s) 833
AsuHPI GGTGA 1 cut(s) 19
AvaII GGWCC 2 cut(s) 79, 732
AxyI CCTNAGG 1 cut(s) 540
BaeGI GKGCMC 1 cut(s) 878
BalI TGGCCA 1 cut(s) 249
BanII GRGCYC 1 cut(s) 198
BbsI GAAGAC 2 cut(s) 707, 820
Bbv12I GWGCWC 2 cut(s) 198, 878
BbvI GCAGC 6 cut(s) 158, 254, 257, 630, 644, 1012
BccI CCATC 1 cut(s) 760
BceAI ACGGC 4 cut(s) 220, 816, 871, 890
BciT130I CCWGG 1 cut(s) 645
BcnI CCSGG 1 cut(s) 833
BcoDI GTCTC 3 cut(s) 476, 683, 776
BfaI CTAG 1 cut(s) 902
BfmI CTRYAG 1 cut(s) 345
BfuAI ACCTGC 1 cut(s) 542
BglI GCCNNNNNGGC 1 cut(s) 339
BisI GCNGC 9 cut(s) 172, 265, 268, 271, 285, 331, 619, 658, 1001
BlsI GCNGC 9 cut(s) 173, 266, 269, 272, 286, 332, 620, 659, 1002
BmcAI AGTACT 1 cut(s) 367
Bme1390I CCNGG 2 cut(s) 645, 833
Bme18I GGWCC 2 cut(s) 79, 732
BmgT120I GGNCC 3 cut(s) 79, 341, 732
BmiI GGNNCC 1 cut(s) 81
BmrFI CCNGG 2 cut(s) 645, 833
BmrI ACTGGG 3 cut(s) 77, 269, 329
BmsI GCATC 1 cut(s) 954
BmuI ACTGGG 3 cut(s) 77, 269, 329
BpiI GAAGAC 2 cut(s) 707, 820
BpuEI CTTGAG 1 cut(s) 634
BpuMI CCSGG 1 cut(s) 833
BsaI GGTCTC 2 cut(s) 476, 776
BsaJI CCNNGG 3 cut(s) 93, 202, 643
BsaXI ACNNNNNCTCC 2 cut(s) 168, 198
Bsc4I CCNNNNNNNGG 6 cut(s) 88, 280, 349, 650, 795, 831
Bse118I RCCGGY 2 cut(s) 88, 167
Bse1I ACTGG 4 cut(s) 72, 250, 275, 324
Bse21I CCTNAGG 1 cut(s) 540
Bse3DI GCAATG 1 cut(s) 856
BseBI CCWGG 1 cut(s) 645
BseDI CCNNGG 3 cut(s) 93, 202, 643
BseGI GGATG 3 cut(s) 586, 821, 945
BseLI CCNNNNNNNGG 6 cut(s) 88, 280, 349, 650, 795, 831
BseMI GCAATG 1 cut(s) 856
BseMII CTCAG 4 cut(s) 426, 531, 881, 939
BseNI ACTGG 4 cut(s) 72, 250, 275, 324
BseSI GKGCMC 1 cut(s) 878
BseXI GCAGC 6 cut(s) 158, 254, 257, 630, 644, 1012
BseYI CCCAGC 1 cut(s) 127
BsgI GTGCAG 3 cut(s) 119, 300, 955
BshFI GGCC 6 cut(s) 92, 249, 302, 333, 342, 803
BsiHKAI GWGCWC 2 cut(s) 198, 878
BsiSI CCGG 3 cut(s) 89, 168, 832
BslI CCNNNNNNNGG 6 cut(s) 88, 280, 349, 650, 795, 831
BsmAI GTCTC 3 cut(s) 476, 683, 776
BsmI GAATGC 2 cut(s) 143, 900
BsnI GGCC 6 cut(s) 92, 249, 302, 333, 342, 803
Bso31I GGTCTC 2 cut(s) 476, 776
Bsp1286I GDGCHC 2 cut(s) 198, 878
Bsp143I GATC 3 cut(s) 513, 862, 928
BspACI CCGC 8 cut(s) 225, 265, 284, 309, 330, 353, 381, 392
BspANI GGCC 6 cut(s) 92, 249, 302, 333, 342, 803
BspCNI CTCAG 4 cut(s) 425, 532, 880, 938
BspLI GGNNCC 1 cut(s) 81
BspMAI CTGCAG 1 cut(s) 349
BspMI ACCTGC 1 cut(s) 542
BspPI GGATC 2 cut(s) 521, 857
BspQI GCTCTTC 1 cut(s) 203
BspTNI GGTCTC 2 cut(s) 476, 776
BsrDI GCAATG 1 cut(s) 856
BsrFI RCCGGY 2 cut(s) 88, 167
BsrI ACTGG 4 cut(s) 72, 250, 275, 324
BssAI RCCGGY 2 cut(s) 88, 167
BssECI CCNNGG 3 cut(s) 93, 202, 643
BssMI GATC 3 cut(s) 513, 862, 928
BssT1I CCWWGG 1 cut(s) 93
Bst2UI CCWGG 1 cut(s) 645
Bst4CI ACNGT 8 cut(s) 60, 362, 509, 677, 758, 817, 887, 976
Bst6I CTCTTC 4 cut(s) 29, 50, 203, 943
BstC8I GCNNGC 4 cut(s) 90, 169, 220, 282
BstDEI CTNAG 4 cut(s) 412, 540, 867, 925
BstDSI CCRYGG 1 cut(s) 202
BstF5I GGATG 3 cut(s) 586, 821, 945
BstKTI GATC 3 cut(s) 516, 865, 931
BstMAI GTCTC 3 cut(s) 476, 683, 776
BstMBI GATC 3 cut(s) 513, 862, 928
BstNI CCWGG 1 cut(s) 645
BstNSI RCATGY 1 cut(s) 811
BstSCI CCNGG 2 cut(s) 643, 831
BstSFI CTRYAG 1 cut(s) 345
BstSLI GKGCMC 1 cut(s) 878
BstV1I GCAGC 6 cut(s) 158, 254, 257, 630, 644, 1012
BstV2I GAAGAC 2 cut(s) 707, 820
BstXI CCANNNNNNTGG 1 cut(s) 319
Bsu36I CCTNAGG 1 cut(s) 540
BsuRI GGCC 6 cut(s) 92, 249, 302, 333, 342, 803
BtgI CCRYGG 1 cut(s) 202
BtsCI GGATG 3 cut(s) 586, 821, 945
BtsI GCAGTG 3 cut(s) 312, 354, 395
BveI ACCTGC 1 cut(s) 542
Cac8I GCNNGC 4 cut(s) 90, 169, 220, 282
CaiI CAGNNNCTG 1 cut(s) 267
Cfr10I RCCGGY 2 cut(s) 88, 167
Cfr13I GGNCC 3 cut(s) 79, 341, 732
Csp6I GTAC 2 cut(s) 366, 624
CviAII CATG 4 cut(s) 493, 777, 808, 841
CviQI GTAC 2 cut(s) 366, 624
DdeI CTNAG 4 cut(s) 412, 540, 867, 925
DpnI GATC 3 cut(s) 515, 864, 930
DpnII GATC 3 cut(s) 513, 862, 928
EaeI YGGCCR 2 cut(s) 247, 331
Eam1104I CTCTTC 4 cut(s) 29, 50, 203, 943
EarI CTCTTC 4 cut(s) 29, 50, 203, 943
EciI GGCGGA 2 cut(s) 214, 298
Ecl136II GAGCTC 1 cut(s) 196
Eco130I CCWWGG 1 cut(s) 93
Eco24I GRGCYC 1 cut(s) 198
Eco31I GGTCTC 2 cut(s) 476, 776
Eco47I GGWCC 2 cut(s) 79, 732
Eco53kI GAGCTC 1 cut(s) 196
Eco57I CTGAAG 1 cut(s) 164
Eco81I CCTNAGG 1 cut(s) 540
EcoICRI GAGCTC 1 cut(s) 196
EcoO109I RGGNCCY 1 cut(s) 341
EcoRII CCWGG 1 cut(s) 643
EcoT14I CCWWGG 1 cut(s) 93
EcoT38I GRGCYC 1 cut(s) 198
ErhI CCWWGG 1 cut(s) 93
FaeI CATG 4 cut(s) 496, 780, 811, 844
FaiI YATR 8 cut(s) 164, 295, 387, 494, 778, 809, 842, 996
FalI AAGNNNNNCTT 6 cut(s) 77, 109, 555, 587, 596, 628
FatI CATG 4 cut(s) 492, 776, 807, 840
FauI CCCGC 2 cut(s) 346, 399
Fnu4HI GCNGC 9 cut(s) 172, 265, 268, 271, 285, 331, 619, 658, 1001
FokI GGATG 3 cut(s) 593, 808, 932
FriOI GRGCYC 1 cut(s) 198
Fsp4HI GCNGC 9 cut(s) 172, 265, 268, 271, 285, 331, 619, 658, 1001
FspBI CTAG 1 cut(s) 902
GluI GCNGC 9 cut(s) 172, 265, 268, 271, 285, 331, 619, 658, 1001
GsaI CCCAGC 1 cut(s) 131
HaeIII GGCC 6 cut(s) 92, 249, 302, 333, 342, 803
HapII CCGG 3 cut(s) 89, 168, 832
Hin1II CATG 4 cut(s) 496, 780, 811, 844
HinfI GANTC 3 cut(s) 410, 793, 911
HpaII CCGG 3 cut(s) 89, 168, 832
HphI GGTGA 1 cut(s) 19
Hpy166II GTNNAC 2 cut(s) 556, 876
Hpy188I TCNGA 7 cut(s) 79, 415, 518, 798, 862, 919, 928
Hpy188III TCNNGA 3 cut(s) 404, 428, 592
Hpy8I GTNNAC 2 cut(s) 556, 876
Hpy99I CGWCG 1 cut(s) 911
HpyAV CCTTC 3 cut(s) 40, 119, 695
HpyCH4III ACNGT 8 cut(s) 60, 362, 509, 677, 758, 817, 887, 976
HpyF3I CTNAG 4 cut(s) 412, 540, 867, 925
Hsp92II CATG 4 cut(s) 496, 780, 811, 844
KroI GCCGGC 2 cut(s) 88, 167
KroNI GCCGGC 2 cut(s) 90, 169
Kzo9I GATC 3 cut(s) 513, 862, 928
LguI GCTCTTC 1 cut(s) 203
LmnI GCTCC 3 cut(s) 189, 227, 665
Lsp1109I GCAGC 6 cut(s) 158, 254, 257, 630, 644, 1012
LweI GCATC 1 cut(s) 954
MaeI CTAG 1 cut(s) 902
MaeIII GTNAC 6 cut(s) 54, 356, 488, 817, 887, 978
MalI GATC 3 cut(s) 515, 864, 930
MboI GATC 3 cut(s) 513, 862, 928
MboII GAAGA 9 cut(s) 16, 37, 111, 190, 616, 709, 712, 820, 930
MhlI GDGCHC 2 cut(s) 198, 878
MlsI TGGCCA 1 cut(s) 249
MluCI AATT 2 cut(s) 607, 709
MluNI TGGCCA 1 cut(s) 249
MlyI GAGTC 1 cut(s) 404
MmeI TCCRAC 2 cut(s) 183, 579
MnlI CCTC 8 cut(s) 16, 53, 73, 535, 718, 876, 909, 946
Mox20I TGGCCA 1 cut(s) 249
MroNI GCCGGC 2 cut(s) 88, 167
MscI TGGCCA 1 cut(s) 249
MseI TTAA 1 cut(s) 690
MslI CAYNNNNRTG 1 cut(s) 740
Msp20I TGGCCA 1 cut(s) 249
MspA1I CMGCKG 2 cut(s) 267, 763
MspI CCGG 3 cut(s) 89, 168, 832
MspR9I CCNGG 2 cut(s) 645, 833
Mva1269I GAATGC 2 cut(s) 143, 900
MvaI CCWGG 1 cut(s) 645
NaeI GCCGGC 2 cut(s) 90, 169
NciI CCSGG 1 cut(s) 833
NdeII GATC 3 cut(s) 513, 862, 928
NgoMIV GCCGGC 2 cut(s) 88, 167
NlaIII CATG 4 cut(s) 496, 780, 811, 844
NlaIV GGNNCC 1 cut(s) 81
NmeAIII GCCGAG 1 cut(s) 278
NmuCI GTSAC 4 cut(s) 356, 488, 887, 978
NspI RCATGY 1 cut(s) 811
PciSI GCTCTTC 1 cut(s) 203
PctI GAATGC 2 cut(s) 143, 900
PdiI GCCGGC 2 cut(s) 90, 169
PfeI GAWTC 2 cut(s) 793, 911
PflFI GACNNNGTC 1 cut(s) 494
PkrI GCNGC 9 cut(s) 173, 266, 269, 272, 286, 332, 620, 659, 1002
PleI GAGTC 1 cut(s) 404
PpsI GAGTC 1 cut(s) 404
Psp124BI GAGCTC 1 cut(s) 198
Psp6I CCWGG 1 cut(s) 643
PspFI CCCAGC 1 cut(s) 127
PspGI CCWGG 1 cut(s) 643
PspN4I GGNNCC 1 cut(s) 81
PspPI GGNCC 3 cut(s) 79, 341, 732
PsrI GAACNNNNNNTAC 2 cut(s) 665, 697
PstI CTGCAG 1 cut(s) 349
PstNI CAGNNNCTG 1 cut(s) 267
PsyI GACNNNGTC 1 cut(s) 494
PvuII CAGCTG 1 cut(s) 763
RsaI GTAC 2 cut(s) 367, 625
RsaNI GTAC 2 cut(s) 366, 624
RseI CAYNNNNRTG 1 cut(s) 740
SacI GAGCTC 1 cut(s) 198
SapI GCTCTTC 1 cut(s) 203
SaqAI TTAA 1 cut(s) 690
SatI GCNGC 9 cut(s) 172, 265, 268, 271, 285, 331, 619, 658, 1001
Sau3AI GATC 3 cut(s) 513, 862, 928
Sau96I GGNCC 3 cut(s) 79, 341, 732
ScaI AGTACT 1 cut(s) 367
SchI GAGTC 1 cut(s) 404
ScrFI CCNGG 2 cut(s) 645, 833
SduI GDGCHC 2 cut(s) 198, 878
SfaNI GCATC 1 cut(s) 954
SfcI CTRYAG 1 cut(s) 345
SfiI GGCCNNNNNGGCC 1 cut(s) 339
SinI GGWCC 2 cut(s) 79, 732
SmiMI CAYNNNNRTG 1 cut(s) 740
SmlI CTYRAG 1 cut(s) 613
SmoI CTYRAG 1 cut(s) 613
Sse9I AATT 2 cut(s) 607, 709
SsiI CCGC 8 cut(s) 225, 265, 284, 309, 330, 353, 381, 392
SspMI CTAG 1 cut(s) 902
SstI GAGCTC 1 cut(s) 198
StyD4I CCNGG 2 cut(s) 643, 831
StyI CCWWGG 1 cut(s) 93
TaaI ACNGT 8 cut(s) 60, 362, 509, 677, 758, 817, 887, 976
TaqI TCGA 1 cut(s) 909
TasI AATT 2 cut(s) 607, 709
TatI WGTACW 1 cut(s) 365
TauI GCSGC 3 cut(s) 267, 287, 333
TfiI GAWTC 2 cut(s) 793, 911
Tru1I TTAA 1 cut(s) 690
Tru9I TTAA 1 cut(s) 690
TseFI GTSAC 4 cut(s) 356, 488, 887, 978
TseI GCWGC 6 cut(s) 171, 267, 270, 618, 657, 1000
Tsp45I GTSAC 4 cut(s) 356, 488, 887, 978
TspDTI ATGAA 1 cut(s) 485
TspGWI ACGGA 2 cut(s) 814, 906
Tth111I GACNNNGTC 1 cut(s) 494
VneI GTGCAC 1 cut(s) 874
VpaK11BI GGWCC 2 cut(s) 79, 732
XceI RCATGY 1 cut(s) 811
XspI CTAG 1 cut(s) 902
ZrmI AGTACT 1 cut(s) 367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.