Rmu_sc0002320.1_g000008

GPI-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002320.1
OG0012959
Physical Location & Seq
Forward (+)
41453 .. 43182
1730 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002320.1_g000008.1.cds

Sequence Viewer

Length: 1014 bp
atgtcctcaaacccaccaccactcttcaccttcatttgcttcctcttcactttgttaccctcctcactgggtctatcggacccgaagccggccttggtgcagtcattccttcccgaatcttctcccccagctacaatccctgcattcccagagcaatccgacatagccggctgcccactccagctccctgatgagctcttccacggcgtaaaggaagcctgctccgccaaaaaaggcgacccgactggccatttacagcacagccgctgctgcccagtgctggcggcgtggctatactcggcctactccgccactgcactgggcagaagcggccaagcaggccctgcattggcgggtcacggtagtactactactaccgcttatgacccgccactgctgcctgatgactcagaaacttgtgttcaggacttgggaaaggcactgaacgcaaaaggaatagagctgatgaaaccaaatgagacctgtgacatggtctattgctactgtgggatcagattgcaccctttgacctgccctgaggcattttcagtgaaccaaaaagggaaacttgttggggatgagagtgtcaagaagttggagaagaattgcttgagcagcagtaccaatgtcaataagttccctggtcttggtggctgctccaaatgcctgaacagtctctaccttcttaacaagaagaagacttccaattcaagcaaaatggaggacaggaccaccaaaatgcacaacaaagactgtcagctgatgggtctcacatggctccttgccaagaatcggacggcctacatgcacaccgttacatccgtcttccgggctatcatgataagcaatgacggctctgatcctcagtcgtgcactctcaacagtgacggaatgcctctagccgtcgattcgtcggaaatctcagatcaatcctcttccagcatccttgcagtttcaatcacgttctgcacagtgtcacttactttgttgtatgtgcagcttaccctgtgctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

337

Amino Acids

36.11

Weight (kDa)

6.37

Isoelectric Point (pI)

59.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012959)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18050
fragaria_vesca FvH4_5g17140 FvH4_5g17140
malus_domestica MD06G1230000.v1.1 MD14G1234700.v1.1
prunus_persica Prupe.5G231000_v2.0.a1
pyrus_communis pycom06g20120 pycom14g19570
rosa_chinensis RchiOBHm_Chr7g0178651
rosa_laevigata RLG00000005426
rosa_multiflora Rmu_sc0002320.1_g000008
rosa_roxburghii Rroxscaffold_3G00274740
rosa_rugosa Rorug06G0418700
rosa_samantha Rh7AG017500 Rh7BG017100 Rh7CG017700 Rh7DG017700
rosa_wichuraiana Rw7G001420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 539
AciI CCGC 8 cut(s) 225, 265, 284, 309, 330, 353, 378, 389
AclWI GGATC 2 cut(s) 518, 854
AcoI YGGCCR 2 cut(s) 247, 331
AfaI GTAC 2 cut(s) 367, 622
AfiI CCNNNNNNNGG 6 cut(s) 88, 280, 349, 647, 792, 828
AgsI TTSAA 2 cut(s) 711, 957
AjnI CCWGG 1 cut(s) 640
AjuI GAANNNNNNNTTGG 2 cut(s) 77, 109
AluBI AGCT 6 cut(s) 131, 184, 196, 463, 760, 1000
AluI AGCT 6 cut(s) 131, 184, 196, 463, 760, 1000
Alw21I GWGCWC 2 cut(s) 198, 875
Alw26I GTCTC 3 cut(s) 473, 680, 773
Alw44I GTGCAC 1 cut(s) 871
AlwI GGATC 2 cut(s) 518, 854
AlwNI CAGNNNCTG 2 cut(s) 267, 344
AoxI GGCC 6 cut(s) 90, 247, 300, 331, 340, 798
ApaLI GTGCAC 1 cut(s) 871
ApeKI GCWGC 7 cut(s) 171, 267, 270, 397, 615, 654, 997
AspS9I GGNCC 3 cut(s) 79, 341, 729
AsuC2I CCSGG 1 cut(s) 830
AsuHPI GGTGA 1 cut(s) 19
AvaII GGWCC 2 cut(s) 79, 729
AxyI CCTNAGG 1 cut(s) 537
BaeGI GKGCMC 1 cut(s) 875
BalI TGGCCA 1 cut(s) 249
BanII GRGCYC 1 cut(s) 198
BbsI GAAGAC 2 cut(s) 704, 817
Bbv12I GWGCWC 2 cut(s) 198, 875
BbvI GCAGC 7 cut(s) 158, 254, 257, 384, 627, 641, 1009
BccI CCATC 1 cut(s) 757
BceAI ACGGC 4 cut(s) 220, 813, 868, 887
BciT130I CCWGG 1 cut(s) 642
BcnI CCSGG 1 cut(s) 830
BcoDI GTCTC 3 cut(s) 473, 680, 773
BfaI CTAG 1 cut(s) 899
BfuAI ACCTGC 1 cut(s) 539
BglI GCCNNNNNGGC 1 cut(s) 339
BmcAI AGTACT 1 cut(s) 367
Bme1390I CCNGG 2 cut(s) 642, 830
Bme18I GGWCC 2 cut(s) 79, 729
BmgT120I GGNCC 3 cut(s) 79, 341, 729
BmiI GGNNCC 2 cut(s) 81, 779
BmrFI CCNGG 2 cut(s) 642, 830
BmrI ACTGGG 3 cut(s) 77, 269, 329
BmsI GCATC 1 cut(s) 951
BmuI ACTGGG 3 cut(s) 77, 269, 329
BpiI GAAGAC 2 cut(s) 704, 817
BpmI CTGGAG 1 cut(s) 164
BpuEI CTTGAG 1 cut(s) 631
BpuMI CCSGG 1 cut(s) 830
BsaI GGTCTC 2 cut(s) 473, 773
BsaJI CCNNGG 3 cut(s) 93, 202, 640
BsaXI ACNNNNNCTCC 2 cut(s) 168, 198
Bsc4I CCNNNNNNNGG 6 cut(s) 88, 280, 349, 647, 792, 828
Bse118I RCCGGY 2 cut(s) 88, 167
Bse1I ACTGG 4 cut(s) 72, 250, 275, 324
Bse21I CCTNAGG 1 cut(s) 537
Bse3DI GCAATG 1 cut(s) 853
BseBI CCWGG 1 cut(s) 642
BseDI CCNNGG 3 cut(s) 93, 202, 640
BseGI GGATG 3 cut(s) 583, 818, 942
BseLI CCNNNNNNNGG 6 cut(s) 88, 280, 349, 647, 792, 828
BseMI GCAATG 1 cut(s) 853
BseMII CTCAG 4 cut(s) 423, 528, 878, 936
BseNI ACTGG 4 cut(s) 72, 250, 275, 324
BseRI GAGGAG 1 cut(s) 52
BseSI GKGCMC 1 cut(s) 875
BseXI GCAGC 7 cut(s) 158, 254, 257, 384, 627, 641, 1009
BseYI CCCAGC 1 cut(s) 127
BsgI GTGCAG 3 cut(s) 119, 300, 952
BshFI GGCC 6 cut(s) 92, 249, 302, 333, 342, 800
BsiHKAI GWGCWC 2 cut(s) 198, 875
BsiSI CCGG 3 cut(s) 89, 168, 829
BslI CCNNNNNNNGG 6 cut(s) 88, 280, 349, 647, 792, 828
BsmAI GTCTC 3 cut(s) 473, 680, 773
BsmI GAATGC 2 cut(s) 143, 897
BsnI GGCC 6 cut(s) 92, 249, 302, 333, 342, 800
Bso31I GGTCTC 2 cut(s) 473, 773
Bsp1286I GDGCHC 2 cut(s) 198, 875
Bsp143I GATC 3 cut(s) 510, 859, 925
BspACI CCGC 8 cut(s) 225, 265, 284, 309, 330, 353, 378, 389
BspANI GGCC 6 cut(s) 92, 249, 302, 333, 342, 800
BspCNI CTCAG 4 cut(s) 422, 529, 877, 935
BspHI TCATGA 1 cut(s) 837
BspLI GGNNCC 2 cut(s) 81, 779
BspMI ACCTGC 1 cut(s) 539
BspPI GGATC 2 cut(s) 518, 854
BspQI GCTCTTC 1 cut(s) 203
BspTNI GGTCTC 2 cut(s) 473, 773
BsrDI GCAATG 1 cut(s) 853
BsrFI RCCGGY 2 cut(s) 88, 167
BsrI ACTGG 4 cut(s) 72, 250, 275, 324
BssAI RCCGGY 2 cut(s) 88, 167
BssECI CCNNGG 3 cut(s) 93, 202, 640
BssMI GATC 3 cut(s) 510, 859, 925
BssT1I CCWWGG 1 cut(s) 93
Bst2UI CCWGG 1 cut(s) 642
Bst4CI ACNGT 7 cut(s) 362, 506, 674, 755, 814, 884, 973
Bst6I CTCTTC 4 cut(s) 29, 50, 203, 940
BstAPI GCANNNNNTGC 1 cut(s) 344
BstC8I GCNNGC 5 cut(s) 90, 169, 220, 282, 340
BstDEI CTNAG 4 cut(s) 409, 537, 864, 922
BstDSI CCRYGG 1 cut(s) 202
BstF5I GGATG 3 cut(s) 583, 818, 942
BstKTI GATC 3 cut(s) 513, 862, 928
BstMAI GTCTC 3 cut(s) 473, 680, 773
BstMBI GATC 3 cut(s) 510, 859, 925
BstNI CCWGG 1 cut(s) 642
BstNSI RCATGY 1 cut(s) 808
BstSCI CCNGG 2 cut(s) 640, 828
BstSLI GKGCMC 1 cut(s) 875
BstV1I GCAGC 7 cut(s) 158, 254, 257, 384, 627, 641, 1009
BstV2I GAAGAC 2 cut(s) 704, 817
BstXI CCANNNNNNTGG 1 cut(s) 319
Bsu36I CCTNAGG 1 cut(s) 537
BsuRI GGCC 6 cut(s) 92, 249, 302, 333, 342, 800
BtgI CCRYGG 1 cut(s) 202
BtsCI GGATG 3 cut(s) 583, 818, 942
BtsI GCAGTG 2 cut(s) 312, 392
BtsIMutI CAGTG 9 cut(s) 65, 282, 312, 317, 392, 440, 555, 889, 978
BveI ACCTGC 1 cut(s) 539
Cac8I GCNNGC 5 cut(s) 90, 169, 220, 282, 340
CaiI CAGNNNCTG 2 cut(s) 267, 344
CciI TCATGA 1 cut(s) 837
Cfr10I RCCGGY 2 cut(s) 88, 167
Cfr13I GGNCC 3 cut(s) 79, 341, 729
Csp6I GTAC 2 cut(s) 366, 621
CviAII CATG 4 cut(s) 490, 774, 805, 838
CviQI GTAC 2 cut(s) 366, 621
DdeI CTNAG 4 cut(s) 409, 537, 864, 922
DpnI GATC 3 cut(s) 512, 861, 927
DpnII GATC 3 cut(s) 510, 859, 925
EaeI YGGCCR 2 cut(s) 247, 331
Eam1104I CTCTTC 4 cut(s) 29, 50, 203, 940
EarI CTCTTC 4 cut(s) 29, 50, 203, 940
EciI GGCGGA 2 cut(s) 214, 298
Ecl136II GAGCTC 1 cut(s) 196
Eco130I CCWWGG 1 cut(s) 93
Eco24I GRGCYC 1 cut(s) 198
Eco31I GGTCTC 2 cut(s) 473, 773
Eco47I GGWCC 2 cut(s) 79, 729
Eco53kI GAGCTC 1 cut(s) 196
Eco81I CCTNAGG 1 cut(s) 537
EcoICRI GAGCTC 1 cut(s) 196
EcoO109I RGGNCCY 1 cut(s) 341
EcoRII CCWGG 1 cut(s) 640
EcoT14I CCWWGG 1 cut(s) 93
EcoT38I GRGCYC 1 cut(s) 198
ErhI CCWWGG 1 cut(s) 93
FaeI CATG 4 cut(s) 493, 777, 808, 841
FaiI YATR 8 cut(s) 164, 295, 384, 491, 775, 806, 839, 993
FalI AAGNNNNNCTT 6 cut(s) 77, 109, 552, 584, 593, 625
FatI CATG 4 cut(s) 489, 773, 804, 837
FauI CCCGC 2 cut(s) 346, 396
FokI GGATG 3 cut(s) 590, 805, 929
FriOI GRGCYC 1 cut(s) 198
FspBI CTAG 1 cut(s) 899
GsaI CCCAGC 1 cut(s) 131
GsuI CTGGAG 1 cut(s) 164
HaeIII GGCC 6 cut(s) 92, 249, 302, 333, 342, 800
HapII CCGG 3 cut(s) 89, 168, 829
Hin1II CATG 4 cut(s) 493, 777, 808, 841
HinfI GANTC 4 cut(s) 116, 407, 790, 908
HpaII CCGG 3 cut(s) 89, 168, 829
HphI GGTGA 1 cut(s) 19
Hpy166II GTNNAC 2 cut(s) 553, 873
Hpy188I TCNGA 8 cut(s) 79, 160, 412, 515, 795, 859, 916, 925
Hpy188III TCNNGA 4 cut(s) 113, 425, 589, 838
Hpy8I GTNNAC 2 cut(s) 553, 873
Hpy99I CGWCG 2 cut(s) 908, 916
HpyAV CCTTC 3 cut(s) 40, 119, 692
HpyCH4III ACNGT 7 cut(s) 362, 506, 674, 755, 814, 884, 973
HpyCH4IV ACGT 1 cut(s) 962
HpyF3I CTNAG 4 cut(s) 409, 537, 864, 922
HpySE526I ACGT 1 cut(s) 962
Hsp92II CATG 4 cut(s) 493, 777, 808, 841
KroI GCCGGC 2 cut(s) 88, 167
KroNI GCCGGC 2 cut(s) 90, 169
Kzo9I GATC 3 cut(s) 510, 859, 925
LguI GCTCTTC 1 cut(s) 203
LmnI GCTCC 4 cut(s) 189, 227, 662, 783
Lsp1109I GCAGC 7 cut(s) 158, 254, 257, 384, 627, 641, 1009
LweI GCATC 1 cut(s) 951
MaeI CTAG 1 cut(s) 899
MaeII ACGT 1 cut(s) 962
MaeIII GTNAC 6 cut(s) 54, 356, 485, 814, 884, 975
MalI GATC 3 cut(s) 512, 861, 927
MboI GATC 3 cut(s) 510, 859, 925
MboII GAAGA 9 cut(s) 16, 37, 111, 190, 613, 706, 709, 817, 927
MhlI GDGCHC 2 cut(s) 198, 875
MlsI TGGCCA 1 cut(s) 249
MluCI AATT 2 cut(s) 604, 706
MluNI TGGCCA 1 cut(s) 249
MlyI GAGTC 1 cut(s) 401
MmeI TCCRAC 3 cut(s) 183, 576, 894
MnlI CCTC 9 cut(s) 16, 53, 70, 73, 532, 715, 873, 906, 943
Mox20I TGGCCA 1 cut(s) 249
MroNI GCCGGC 2 cut(s) 88, 167
MscI TGGCCA 1 cut(s) 249
MseI TTAA 1 cut(s) 687
MslI CAYNNNNRTG 1 cut(s) 737
Msp20I TGGCCA 1 cut(s) 249
MspA1I CMGCKG 2 cut(s) 267, 760
MspI CCGG 3 cut(s) 89, 168, 829
MspR9I CCNGG 2 cut(s) 642, 830
Mva1269I GAATGC 2 cut(s) 143, 897
MvaI CCWGG 1 cut(s) 642
NaeI GCCGGC 2 cut(s) 90, 169
NciI CCSGG 1 cut(s) 830
NdeII GATC 3 cut(s) 510, 859, 925
NgoMIV GCCGGC 2 cut(s) 88, 167
NlaIII CATG 4 cut(s) 493, 777, 808, 841
NlaIV GGNNCC 2 cut(s) 81, 779
NmeAIII GCCGAG 1 cut(s) 278
NmuCI GTSAC 4 cut(s) 356, 485, 884, 975
NspI RCATGY 1 cut(s) 808
PagI TCATGA 1 cut(s) 837
PciSI GCTCTTC 1 cut(s) 203
PctI GAATGC 2 cut(s) 143, 897
PdiI GCCGGC 2 cut(s) 90, 169
PfeI GAWTC 3 cut(s) 116, 790, 908
PflFI GACNNNGTC 1 cut(s) 491
PleI GAGTC 1 cut(s) 401
PpsI GAGTC 1 cut(s) 401
Psp124BI GAGCTC 1 cut(s) 198
Psp6I CCWGG 1 cut(s) 640
PspFI CCCAGC 1 cut(s) 127
PspGI CCWGG 1 cut(s) 640
PspN4I GGNNCC 2 cut(s) 81, 779
PspPI GGNCC 3 cut(s) 79, 341, 729
PsrI GAACNNNNNNTAC 2 cut(s) 662, 694
PstNI CAGNNNCTG 2 cut(s) 267, 344
PsyI GACNNNGTC 1 cut(s) 491
PvuII CAGCTG 1 cut(s) 760
RsaI GTAC 2 cut(s) 367, 622
RsaNI GTAC 2 cut(s) 366, 621
RseI CAYNNNNRTG 1 cut(s) 737
SacI GAGCTC 1 cut(s) 198
SapI GCTCTTC 1 cut(s) 203
SaqAI TTAA 1 cut(s) 687
Sau3AI GATC 3 cut(s) 510, 859, 925
Sau96I GGNCC 3 cut(s) 79, 341, 729
ScaI AGTACT 1 cut(s) 367
SchI GAGTC 1 cut(s) 401
ScrFI CCNGG 2 cut(s) 642, 830
SduI GDGCHC 2 cut(s) 198, 875
SfaNI GCATC 1 cut(s) 951
SfiI GGCCNNNNNGGCC 1 cut(s) 339
SinI GGWCC 2 cut(s) 79, 729
SmiMI CAYNNNNRTG 1 cut(s) 737
SmlI CTYRAG 1 cut(s) 610
SmoI CTYRAG 1 cut(s) 610
Sse9I AATT 2 cut(s) 604, 706
SsiI CCGC 8 cut(s) 225, 265, 284, 309, 330, 353, 378, 389
SspMI CTAG 1 cut(s) 899
SstI GAGCTC 1 cut(s) 198
StyD4I CCNGG 2 cut(s) 640, 828
StyI CCWWGG 1 cut(s) 93
TaaI ACNGT 7 cut(s) 362, 506, 674, 755, 814, 884, 973
TaiI ACGT 1 cut(s) 965
TaqI TCGA 1 cut(s) 906
TasI AATT 2 cut(s) 604, 706
TatI WGTACW 1 cut(s) 365
TauI GCSGC 3 cut(s) 267, 287, 333
TfiI GAWTC 3 cut(s) 116, 790, 908
Tru1I TTAA 1 cut(s) 687
Tru9I TTAA 1 cut(s) 687
TscAI CASTG 9 cut(s) 72, 282, 319, 324, 399, 447, 555, 889, 978
TseFI GTSAC 4 cut(s) 356, 485, 884, 975
TseI GCWGC 7 cut(s) 171, 267, 270, 397, 615, 654, 997
Tsp45I GTSAC 4 cut(s) 356, 485, 884, 975
TspDTI ATGAA 2 cut(s) 22, 482
TspGWI ACGGA 2 cut(s) 811, 903
TspRI CASTG 9 cut(s) 72, 282, 319, 324, 399, 447, 555, 889, 978
Tth111I GACNNNGTC 1 cut(s) 491
VneI GTGCAC 1 cut(s) 871
VpaK11BI GGWCC 2 cut(s) 79, 729
XceI RCATGY 1 cut(s) 808
XspI CTAG 1 cut(s) 899
ZrmI AGTACT 1 cut(s) 367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.