FvH4_5g17140

GPI-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0012959
Physical Location & Seq
Reverse (-)
9783107 .. 9785438
2332 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g17140.t2

Sequence Viewer

Length: 1014 bp
ATGTCCCCAAACCCACCTCCCCTCTTCCCCTTCCTTTGCCTCCTCTTCACTCTCTTACCCTCCTCACTGGCACTAACCAATCCGGACCCGGCTGCAGTGCAGCCATTCCTTCCTAAATCTTCTCCCCCAGCTACAATCCCTGCATTCCCAGAGCAATCCGACATAGCCGGCTGCCCACTCCAGCTCCCTGATGAGCTCTTCCACGGCGTAAAGGCCGCCTGCTCAGCCAAAAAAGGCGACCCGGCTGGCCATTTACAGCACAGCCGCTGCTGCCCTGTGCTCGCGGCGTGGCTCTACTCGGCCTACTCTGCCACCGCATTGGGCAGAAGCGGCCGAGTAGGGCCTGCAGTAACGGGTCACGGTAGTAATACTACTACCGCGTATGACCCGCCACTGCTGCCTGATGACTCGGAAACTTGTGTTCAGGACTTGGGAAAAGCGCTGAGTGCAAAGGGAATAGAGCTGATGAGACCAAACGAGACCTGTGACATGGTGTACTGCAACTGTGGGATCAGATTGCATCCTTTGAGCTGCCCTGAGGCATTTTCAGTGAACCAAAATGGGAAACTTATTGGGGATAAGAGTGTCAAGAAGTTAGAGAAGAACTGCTTGAGCAGCAGCAACAATGTCAACAATTTCCCTGGTCTTGGTGGCTGCTCCAAATGCCTCAACAGTCTCAAACTTCTTAACAAGAAGAAGACTTCCAATTCAAGCAAATTGGAGGACAGGACCACCAAAATGCACAACAAAGATTGTCAGCTGATGGGTCTCACATGGCTCCTTGCCAAGAATCGGACGGCCTACATGCACACCGTTACATCCGTCTTCCGGGCTATCATGGTAAGCAACGACGGCTCCGATCCTCAGTCGTGCACCCTCAACAGTGACGGAATGCCTCTAGCTGTCGATTCGTCGGAAATCTCTGATCAGTCCCCCTCCAACATCCTTGCAGTTTCATTCACATTCTGCGTAGTGTCACTTTCTCTGTTGTATGTGCAGCTTACCATGAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

338

Amino Acids

35.98

Weight (kDa)

7.84

Isoelectric Point (pI)

59.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPARK PF19160 56 - 231 9e-08 SPARK
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012959)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18050
fragaria_vesca FvH4_5g17140 FvH4_5g17140
malus_domestica MD06G1230000.v1.1 MD14G1234700.v1.1
prunus_persica Prupe.5G231000_v2.0.a1
pyrus_communis pycom06g20120 pycom14g19570
rosa_chinensis RchiOBHm_Chr7g0178651
rosa_laevigata RLG00000005426
rosa_multiflora Rmu_sc0002320.1_g000008
rosa_roxburghii Rroxscaffold_3G00274740
rosa_rugosa Rorug06G0418700
rosa_samantha Rh7AG017500 Rh7BG017100 Rh7CG017700 Rh7DG017700
rosa_wichuraiana Rw7G001420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 284, 380
AccIII TCCGGA 1 cut(s) 82
AciI CCGC 7 cut(s) 216, 265, 284, 315, 330, 378, 389
AclWI GGATC 2 cut(s) 518, 854
AcoI YGGCCR 2 cut(s) 247, 331
AfaI GTAC 1 cut(s) 497
AfeI AGCGCT 1 cut(s) 441
AfiI CCNNNNNNNGG 3 cut(s) 647, 792, 828
AgsI TTSAA 1 cut(s) 711
AjnI CCWGG 1 cut(s) 640
AluBI AGCT 9 cut(s) 131, 184, 196, 463, 531, 760, 902, 1000, 1011
AluI AGCT 9 cut(s) 131, 184, 196, 463, 531, 760, 902, 1000, 1011
Alw21I GWGCWC 3 cut(s) 198, 282, 875
Alw26I GTCTC 4 cut(s) 463, 473, 680, 773
Alw44I GTGCAC 1 cut(s) 871
AlwI GGATC 2 cut(s) 518, 854
AlwNI CAGNNNCTG 1 cut(s) 267
Aor13HI TCCGGA 1 cut(s) 82
Aor51HI AGCGCT 1 cut(s) 441
AoxI GGCC 6 cut(s) 213, 247, 300, 331, 341, 798
ApaLI GTGCAC 1 cut(s) 871
AspLEI GCGC 1 cut(s) 442
AspS9I GGNCC 3 cut(s) 85, 341, 729
AsuC2I CCSGG 3 cut(s) 89, 242, 830
AvaII GGWCC 2 cut(s) 85, 729
AxyI CCTNAGG 1 cut(s) 537
BaeGI GKGCMC 1 cut(s) 875
BalI TGGCCA 1 cut(s) 249
BanII GRGCYC 1 cut(s) 198
BbsI GAAGAC 2 cut(s) 704, 817
Bbv12I GWGCWC 3 cut(s) 198, 282, 875
BccI CCATC 1 cut(s) 757
BceAI ACGGC 3 cut(s) 220, 813, 868
BciT130I CCWGG 1 cut(s) 642
BclI TGATCA 1 cut(s) 925
BcnI CCSGG 3 cut(s) 89, 242, 830
BcoDI GTCTC 4 cut(s) 463, 473, 680, 773
BfaI CTAG 1 cut(s) 899
BfmI CTRYAG 2 cut(s) 93, 345
BfoI RGCGCY 1 cut(s) 443
BlpI GCTNAGC 1 cut(s) 223
Bme1390I CCNGG 4 cut(s) 89, 242, 642, 830
Bme18I GGWCC 2 cut(s) 85, 729
BmgT120I GGNCC 3 cut(s) 85, 341, 729
BmiI GGNNCC 3 cut(s) 87, 779, 856
BmrFI CCNGG 4 cut(s) 89, 242, 642, 830
BmsI GCATC 1 cut(s) 529
BpiI GAAGAC 2 cut(s) 704, 817
BpmI CTGGAG 1 cut(s) 164
Bpu1102I GCTNAGC 1 cut(s) 223
BpuEI CTTGAG 1 cut(s) 631
BpuMI CCSGG 3 cut(s) 89, 242, 830
BsaI GGTCTC 3 cut(s) 463, 473, 773
BsaJI CCNNGG 2 cut(s) 202, 640
BsaWI WCCGGW 1 cut(s) 82
BsaXI ACNNNNNCTCC 4 cut(s) 168, 198, 839, 869
Bsc4I CCNNNNNNNGG 3 cut(s) 647, 792, 828
Bse118I RCCGGY 1 cut(s) 167
Bse1I ACTGG 1 cut(s) 72
Bse21I CCTNAGG 1 cut(s) 537
BseAI TCCGGA 1 cut(s) 82
BseBI CCWGG 1 cut(s) 642
BseDI CCNNGG 2 cut(s) 202, 640
BseGI GGATG 3 cut(s) 520, 818, 942
BseLI CCNNNNNNNGG 3 cut(s) 647, 792, 828
BseMII CTCAG 4 cut(s) 237, 434, 528, 878
BseNI ACTGG 1 cut(s) 72
BseRI GAGGAG 2 cut(s) 32, 52
BseSI GKGCMC 1 cut(s) 875
BseX3I CGGCCG 1 cut(s) 331
BseYI CCCAGC 1 cut(s) 127
BsgI GTGCAG 1 cut(s) 119
Bsh1236I CGCG 2 cut(s) 284, 380
Bsh1285I CGRYCG 1 cut(s) 334
BshFI GGCC 6 cut(s) 215, 249, 302, 333, 343, 800
BsiEI CGRYCG 1 cut(s) 334
BsiHKAI GWGCWC 3 cut(s) 198, 282, 875
BsiSI CCGG 5 cut(s) 83, 89, 168, 242, 829
BslFI GGGAC 1 cut(s) 916
BslI CCNNNNNNNGG 3 cut(s) 647, 792, 828
BsmAI GTCTC 4 cut(s) 463, 473, 680, 773
BsmFI GGGAC 1 cut(s) 916
BsmI GAATGC 2 cut(s) 143, 897
BsnI GGCC 6 cut(s) 215, 249, 302, 333, 343, 800
Bso31I GGTCTC 3 cut(s) 463, 473, 773
Bsp1286I GDGCHC 3 cut(s) 198, 282, 875
Bsp13I TCCGGA 1 cut(s) 82
Bsp143I GATC 3 cut(s) 510, 859, 925
Bsp1720I GCTNAGC 1 cut(s) 223
BspACI CCGC 7 cut(s) 216, 265, 284, 315, 330, 378, 389
BspANI GGCC 6 cut(s) 215, 249, 302, 333, 343, 800
BspCNI CTCAG 4 cut(s) 236, 435, 529, 877
BspEI TCCGGA 1 cut(s) 82
BspFNI CGCG 2 cut(s) 284, 380
BspLI GGNNCC 3 cut(s) 87, 779, 856
BspMAI CTGCAG 2 cut(s) 97, 349
BspPI GGATC 2 cut(s) 518, 854
BspQI GCTCTTC 1 cut(s) 203
BspTNI GGTCTC 3 cut(s) 463, 473, 773
BsrFI RCCGGY 1 cut(s) 167
BsrI ACTGG 1 cut(s) 72
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 2 cut(s) 202, 640
BssMI GATC 3 cut(s) 510, 859, 925
Bst2UI CCWGG 1 cut(s) 642
Bst4CI ACNGT 5 cut(s) 362, 506, 674, 814, 884
Bst6I CTCTTC 3 cut(s) 29, 50, 203
BstC8I GCNNGC 5 cut(s) 169, 220, 247, 282, 345
BstDEI CTNAG 4 cut(s) 223, 443, 537, 864
BstDSI CCRYGG 1 cut(s) 202
BstF5I GGATG 3 cut(s) 520, 818, 942
BstFNI CGCG 2 cut(s) 284, 380
BstH2I RGCGCY 1 cut(s) 443
BstHHI GCGC 1 cut(s) 442
BstKTI GATC 3 cut(s) 513, 862, 928
BstMAI GTCTC 4 cut(s) 463, 473, 680, 773
BstMBI GATC 3 cut(s) 510, 859, 925
BstMCI CGRYCG 1 cut(s) 334
BstMWI GCNNNNNNNGC 9 cut(s) 224, 270, 308, 330, 397, 446, 615, 663, 852
BstNI CCWGG 1 cut(s) 642
BstNSI RCATGY 1 cut(s) 808
BstSCI CCNGG 4 cut(s) 87, 240, 640, 828
BstSFI CTRYAG 2 cut(s) 93, 345
BstSLI GKGCMC 1 cut(s) 875
BstUI CGCG 2 cut(s) 284, 380
BstV2I GAAGAC 2 cut(s) 704, 817
BstXI CCANNNNNNTGG 1 cut(s) 319
BstZI CGGCCG 1 cut(s) 331
Bsu36I CCTNAGG 1 cut(s) 537
BsuRI GGCC 6 cut(s) 215, 249, 302, 333, 343, 800
BtgI CCRYGG 1 cut(s) 202
BtsCI GGATG 3 cut(s) 520, 818, 942
BtsI GCAGTG 2 cut(s) 102, 392
BtsIMutI CAGTG 5 cut(s) 65, 102, 392, 555, 889
Cac8I GCNNGC 5 cut(s) 169, 220, 247, 282, 345
CaiI CAGNNNCTG 1 cut(s) 267
CfoI GCGC 1 cut(s) 442
Cfr10I RCCGGY 1 cut(s) 167
Cfr13I GGNCC 3 cut(s) 85, 341, 729
Csp6I GTAC 1 cut(s) 496
CviAII CATG 5 cut(s) 490, 774, 805, 838, 1006
CviQI GTAC 1 cut(s) 496
DdeI CTNAG 4 cut(s) 223, 443, 537, 864
DpnI GATC 3 cut(s) 512, 861, 927
DpnII GATC 3 cut(s) 510, 859, 925
EaeI YGGCCR 2 cut(s) 247, 331
EagI CGGCCG 1 cut(s) 331
Eam1104I CTCTTC 3 cut(s) 29, 50, 203
EarI CTCTTC 3 cut(s) 29, 50, 203
Ecl136II GAGCTC 1 cut(s) 196
EclXI CGGCCG 1 cut(s) 331
Eco24I GRGCYC 1 cut(s) 198
Eco31I GGTCTC 3 cut(s) 463, 473, 773
Eco47I GGWCC 2 cut(s) 85, 729
Eco47III AGCGCT 1 cut(s) 441
Eco52I CGGCCG 1 cut(s) 331
Eco53kI GAGCTC 1 cut(s) 196
Eco81I CCTNAGG 1 cut(s) 537
EcoICRI GAGCTC 1 cut(s) 196
EcoO109I RGGNCCY 1 cut(s) 341
EcoRII CCWGG 1 cut(s) 640
EcoT38I GRGCYC 1 cut(s) 198
FaeI CATG 5 cut(s) 493, 777, 808, 841, 1009
FaiI YATR 8 cut(s) 164, 384, 491, 775, 806, 839, 993, 1007
FalI AAGNNNNNCTT 2 cut(s) 593, 625
FaqI GGGAC 1 cut(s) 916
FatI CATG 5 cut(s) 489, 773, 804, 837, 1005
FauI CCCGC 1 cut(s) 396
FbaI TGATCA 1 cut(s) 925
FokI GGATG 3 cut(s) 507, 805, 929
FriOI GRGCYC 1 cut(s) 198
FspBI CTAG 1 cut(s) 899
GlaI GCGC 1 cut(s) 441
GsaI CCCAGC 1 cut(s) 131
GsuI CTGGAG 1 cut(s) 164
HaeII RGCGCY 1 cut(s) 443
HaeIII GGCC 6 cut(s) 215, 249, 302, 333, 343, 800
HapII CCGG 5 cut(s) 83, 89, 168, 242, 829
HhaI GCGC 1 cut(s) 442
Hin1II CATG 5 cut(s) 493, 777, 808, 841, 1009
Hin6I GCGC 1 cut(s) 440
HinP1I GCGC 1 cut(s) 440
HincII GTYRAC 1 cut(s) 631
HindII GTYRAC 1 cut(s) 631
HinfI GANTC 3 cut(s) 407, 790, 908
HpaII CCGG 5 cut(s) 83, 89, 168, 242, 829
Hpy166II GTNNAC 4 cut(s) 496, 553, 631, 873
Hpy188I TCNGA 7 cut(s) 160, 412, 515, 795, 859, 916, 925
Hpy188III TCNNGA 3 cut(s) 83, 425, 589
Hpy8I GTNNAC 4 cut(s) 496, 553, 631, 873
Hpy99I CGWCG 2 cut(s) 854, 916
HpyAV CCTTC 2 cut(s) 40, 119
HpyCH4III ACNGT 5 cut(s) 362, 506, 674, 814, 884
HpyF10VI GCNNNNNNNGC 9 cut(s) 224, 270, 308, 330, 397, 446, 615, 663, 852
HpyF3I CTNAG 4 cut(s) 223, 443, 537, 864
Hsp92II CATG 5 cut(s) 493, 777, 808, 841, 1009
HspAI GCGC 1 cut(s) 440
Kpn2I TCCGGA 1 cut(s) 82
KroI GCCGGC 1 cut(s) 167
KroNI GCCGGC 1 cut(s) 169
Ksp22I TGATCA 1 cut(s) 925
Kzo9I GATC 3 cut(s) 510, 859, 925
LguI GCTCTTC 1 cut(s) 203
LmnI GCTCC 4 cut(s) 189, 662, 783, 860
LweI GCATC 1 cut(s) 529
MaeI CTAG 1 cut(s) 899
MaeIII GTNAC 6 cut(s) 349, 356, 485, 814, 884, 975
MalI GATC 3 cut(s) 512, 861, 927
MboI GATC 3 cut(s) 510, 859, 925
MboII GAAGA 8 cut(s) 16, 37, 111, 190, 613, 706, 709, 817
MhlI GDGCHC 3 cut(s) 198, 282, 875
MlsI TGGCCA 1 cut(s) 249
MluCI AATT 3 cut(s) 634, 706, 716
MluNI TGGCCA 1 cut(s) 249
MlyI GAGTC 1 cut(s) 401
MmeI TCCRAC 3 cut(s) 183, 894, 963
Mox20I TGGCCA 1 cut(s) 249
MroI TCCGGA 1 cut(s) 82
MroNI GCCGGC 1 cut(s) 167
MscI TGGCCA 1 cut(s) 249
MseI TTAA 1 cut(s) 687
MslI CAYNNNNRTG 1 cut(s) 737
Msp20I TGGCCA 1 cut(s) 249
MspA1I CMGCKG 2 cut(s) 267, 760
MspI CCGG 5 cut(s) 83, 89, 168, 242, 829
MspR9I CCNGG 4 cut(s) 89, 242, 642, 830
Mva1269I GAATGC 2 cut(s) 143, 897
MvaI CCWGG 1 cut(s) 642
MvnI CGCG 2 cut(s) 284, 380
MwoI GCNNNNNNNGC 9 cut(s) 224, 270, 308, 330, 397, 446, 615, 663, 852
NaeI GCCGGC 1 cut(s) 169
NciI CCSGG 3 cut(s) 89, 242, 830
NdeII GATC 3 cut(s) 510, 859, 925
NgoMIV GCCGGC 1 cut(s) 167
NlaIII CATG 5 cut(s) 493, 777, 808, 841, 1009
NlaIV GGNNCC 3 cut(s) 87, 779, 856
NmeAIII GCCGAG 2 cut(s) 278, 359
NmuCI GTSAC 4 cut(s) 356, 485, 884, 975
NspI RCATGY 1 cut(s) 808
PciSI GCTCTTC 1 cut(s) 203
PcsI WCGNNNNNNNCGW 1 cut(s) 855
PctI GAATGC 2 cut(s) 143, 897
PdiI GCCGGC 1 cut(s) 169
PfeI GAWTC 2 cut(s) 790, 908
PleI GAGTC 1 cut(s) 401
PpsI GAGTC 1 cut(s) 401
Psp124BI GAGCTC 1 cut(s) 198
Psp6I CCWGG 1 cut(s) 640
PspFI CCCAGC 1 cut(s) 127
PspGI CCWGG 1 cut(s) 640
PspN4I GGNNCC 3 cut(s) 87, 779, 856
PspPI GGNCC 3 cut(s) 85, 341, 729
PstI CTGCAG 2 cut(s) 97, 349
PstNI CAGNNNCTG 1 cut(s) 267
PvuII CAGCTG 1 cut(s) 760
RsaI GTAC 1 cut(s) 497
RsaNI GTAC 1 cut(s) 496
RseI CAYNNNNRTG 1 cut(s) 737
SacI GAGCTC 1 cut(s) 198
SapI GCTCTTC 1 cut(s) 203
SaqAI TTAA 1 cut(s) 687
Sau3AI GATC 3 cut(s) 510, 859, 925
Sau96I GGNCC 3 cut(s) 85, 341, 729
SchI GAGTC 1 cut(s) 401
ScrFI CCNGG 4 cut(s) 89, 242, 642, 830
SduI GDGCHC 3 cut(s) 198, 282, 875
SfaNI GCATC 1 cut(s) 529
SfcI CTRYAG 2 cut(s) 93, 345
SinI GGWCC 2 cut(s) 85, 729
SmiMI CAYNNNNRTG 1 cut(s) 737
SmlI CTYRAG 1 cut(s) 610
SmoI CTYRAG 1 cut(s) 610
Sse9I AATT 3 cut(s) 634, 706, 716
SsiI CCGC 7 cut(s) 216, 265, 284, 315, 330, 378, 389
SspMI CTAG 1 cut(s) 899
SstI GAGCTC 1 cut(s) 198
StyD4I CCNGG 4 cut(s) 87, 240, 640, 828
TaaI ACNGT 5 cut(s) 362, 506, 674, 814, 884
TaqI TCGA 1 cut(s) 906
TasI AATT 3 cut(s) 634, 706, 716
TatI WGTACW 1 cut(s) 495
TauI GCSGC 4 cut(s) 218, 267, 287, 333
TfiI GAWTC 2 cut(s) 790, 908
Tru1I TTAA 1 cut(s) 687
Tru9I TTAA 1 cut(s) 687
TscAI CASTG 5 cut(s) 72, 102, 399, 555, 889
TseFI GTSAC 4 cut(s) 356, 485, 884, 975
Tsp45I GTSAC 4 cut(s) 356, 485, 884, 975
TspDTI ATGAA 1 cut(s) 945
TspGWI ACGGA 2 cut(s) 811, 903
TspRI CASTG 5 cut(s) 72, 102, 399, 555, 889
VneI GTGCAC 1 cut(s) 871
VpaK11BI GGWCC 2 cut(s) 85, 729
XceI RCATGY 1 cut(s) 808
XspI CTAG 1 cut(s) 899
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.